Starting /dee2/code/volunteer_pipeline.sh ERR11006574 current disk space = 1550655873024 free memory = 1600429064 ERR11006574 SRAfilesize b0046cedd9d95b6593b13a13b9331baa ERR11006574.sra ERR11006574.sra file validated ERR11006574 is paired end ERR11006574 is conventional basespace ERR11006574 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006574_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.444 37.0 37.0 37.0 37.0 37.0 2 36.44575 37.0 37.0 37.0 37.0 37.0 3 36.47 37.0 37.0 37.0 37.0 37.0 4 36.6135 37.0 37.0 37.0 37.0 37.0 5 36.5095 37.0 37.0 37.0 37.0 37.0 6 36.5025 37.0 37.0 37.0 37.0 37.0 7 36.4595 37.0 37.0 37.0 37.0 37.0 8 36.4825 37.0 37.0 37.0 37.0 37.0 9 36.468 37.0 37.0 37.0 37.0 37.0 10-14 36.492900000000006 37.0 37.0 37.0 37.0 37.0 15-19 36.534 37.0 37.0 37.0 37.0 37.0 20-24 36.4905 37.0 37.0 37.0 37.0 37.0 25-29 36.3942 37.0 37.0 37.0 37.0 37.0 30-34 36.3785 37.0 37.0 37.0 37.0 37.0 35-39 36.3523 37.0 37.0 37.0 37.0 37.0 40-44 36.363200000000006 37.0 37.0 37.0 37.0 37.0 45-49 36.3749 37.0 37.0 37.0 37.0 37.0 50-54 36.3292 37.0 37.0 37.0 37.0 37.0 55-59 36.2355 37.0 37.0 37.0 37.0 37.0 60-64 36.1909 37.0 37.0 37.0 37.0 37.0 65-69 36.143299999999996 37.0 37.0 37.0 37.0 37.0 70-74 36.154399999999995 37.0 37.0 37.0 37.0 37.0 75-79 36.1126 37.0 37.0 37.0 37.0 37.0 80-84 36.1323 37.0 37.0 37.0 37.0 37.0 85-89 36.1257 37.0 37.0 37.0 37.0 37.0 90-94 36.0235 37.0 37.0 37.0 37.0 37.0 95-99 36.0141 37.0 37.0 37.0 37.0 37.0 100-104 35.972500000000004 37.0 37.0 37.0 37.0 37.0 105-109 35.9499 37.0 37.0 37.0 37.0 37.0 110-114 35.918699999999994 37.0 37.0 37.0 37.0 37.0 115-119 35.9961 37.0 37.0 37.0 37.0 37.0 120-124 35.7936 37.0 37.0 37.0 37.0 37.0 125-129 35.75430000000001 37.0 37.0 37.0 37.0 37.0 130-134 35.7869 37.0 37.0 37.0 37.0 37.0 135-139 35.6252 37.0 37.0 37.0 37.0 37.0 140-144 35.5692 37.0 37.0 37.0 37.0 37.0 145-149 35.3321 37.0 37.0 37.0 29.8 37.0 150 35.4195 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 1.0 24 7.0 25 5.0 26 1.0 27 9.0 28 11.0 29 20.0 30 27.0 31 42.0 32 56.0 33 77.0 34 145.0 35 383.0 36 3050.0 37 165.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.525 11.275 14.575 37.625 2 26.006501625406354 9.352338084521131 26.731682920730183 37.90947736934234 3 21.349999999999998 11.799999999999999 28.175 38.675 4 27.675 14.424999999999999 24.95 32.95 5 26.775 18.525 27.700000000000003 27.0 6 23.549999999999997 27.375 23.400000000000002 25.674999999999997 7 18.85 25.85 34.65 20.65 8 16.45 26.724999999999998 35.15 21.675 9 17.25 26.025 37.5 19.225 10-14 19.97 31.605 27.315 21.11 15-19 20.54 32.690000000000005 25.7 21.07 20-24 19.045 29.830000000000002 28.16 22.965 25-29 21.32 31.19 26.0 21.490000000000002 30-34 23.185 29.455 26.085 21.275 35-39 21.81 31.0 25.965 21.224999999999998 40-44 19.875 29.595 26.015 24.515 45-49 20.349999999999998 28.83 27.415 23.405 50-54 20.59 30.9 26.435 22.075 55-59 21.884999999999998 29.189999999999998 24.08 24.845 60-64 20.445 30.56 25.915 23.080000000000002 65-69 20.115 30.075000000000003 25.590000000000003 24.22 70-74 22.33 29.07 23.965 24.635 75-79 21.92 28.925 25.39 23.765 80-84 22.634999999999998 29.18 24.64 23.544999999999998 85-89 24.015 28.27 24.69 23.025000000000002 90-94 21.415 29.065 27.05 22.470000000000002 95-99 23.94 28.749999999999996 23.91 23.400000000000002 100-104 21.584999999999997 30.464999999999996 24.645 23.305 105-109 22.55 28.084999999999997 25.919999999999998 23.445 110-114 23.915 27.62 24.58 23.885 115-119 20.195 29.21 25.735000000000003 24.86 120-124 20.9 28.610000000000003 24.93 25.56 125-129 21.39 31.330000000000002 22.875 24.404999999999998 130-134 23.27 29.720000000000002 24.75 22.259999999999998 135-139 23.7 28.634999999999998 23.54 24.125 140-144 24.34 28.050000000000004 26.265 21.345 145-149 23.105 29.65 24.955 22.29 150 23.724999999999998 27.575 26.875 21.825 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 1.0 21 2.0 22 4.5 23 5.0 24 6.0 25 6.0 26 6.5 27 8.0 28 12.0 29 16.5 30 14.0 31 19.5 32 27.0 33 29.5 34 42.0 35 60.0 36 81.0 37 147.5 38 264.5 39 295.5 40 249.5 41 253.0 42 243.0 43 228.0 44 219.5 45 215.5 46 217.0 47 174.0 48 122.0 49 92.5 50 88.5 51 81.0 52 58.0 53 48.0 54 46.5 55 41.5 56 33.0 57 28.0 58 30.0 59 32.0 60 37.0 61 37.0 62 37.0 63 30.5 64 31.5 65 49.0 66 39.5 67 25.0 68 23.5 69 16.0 70 18.5 71 18.0 72 14.0 73 15.5 74 12.5 75 11.0 76 10.0 77 6.0 78 4.5 79 4.0 80 4.0 81 2.5 82 1.5 83 1.5 84 0.5 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.95 #Duplication Level Percentage of deduplicated Percentage of total 1 81.74010455563854 54.725 2 11.538461538461538 15.45 3 2.389843166542196 4.8 4 1.6430171769977595 4.3999999999999995 5 0.597460791635549 2.0 6 0.5227781926811054 2.1 7 0.33607169529499625 1.575 8 0.33607169529499625 1.7999999999999998 9 0.0 0.0 >10 0.8588498879761016 9.950000000000001 >50 0.0 0.0 >100 0.03734129947722181 3.2 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 128 3.2 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 34 0.8500000000000001 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 33 0.8250000000000001 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 31 0.775 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 29 0.7250000000000001 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 26 0.65 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 23 0.575 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 19 0.475 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 17 0.42500000000000004 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 17 0.42500000000000004 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 15 0.375 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 15 0.375 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 15 0.375 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 14 0.35000000000000003 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 13 0.325 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 13 0.325 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 11 0.27499999999999997 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 11 0.27499999999999997 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 11 0.27499999999999997 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 11 0.27499999999999997 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 10 0.25 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 10 0.25 No Hit ATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATA 10 0.25 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 10 0.25 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 8 0.2 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 8 0.2 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 8 0.2 No Hit ACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 8 0.2 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 8 0.2 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 8 0.2 No Hit TCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGT 8 0.2 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 8 0.2 No Hit TGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCT 8 0.2 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 7 0.17500000000000002 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 7 0.17500000000000002 No Hit CCCTCTTCAAATAGATCTAATGGATAAGCTACATAACAGATCCATTGACT 7 0.17500000000000002 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 7 0.17500000000000002 No Hit GGCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGAT 7 0.17500000000000002 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 7 0.17500000000000002 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 7 0.17500000000000002 No Hit GCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAGCTGCAA 7 0.17500000000000002 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 7 0.17500000000000002 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 6 0.15 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 6 0.15 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 6 0.15 No Hit ACCCTCTTCAAATAGATCTAATGGATAAGCTACATAACAGATCCATTGAC 6 0.15 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 6 0.15 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 6 0.15 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 6 0.15 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 6 0.15 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 6 0.15 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 6 0.15 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 6 0.15 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 6 0.15 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 6 0.15 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 6 0.15 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 5 0.125 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 5 0.125 No Hit CCCCAGTTAAGTAGTCATGCATTACAATAGGAACACCTAATTCTCTCGCA 5 0.125 No Hit ATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGT 5 0.125 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 5 0.125 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 5 0.125 No Hit TCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGT 5 0.125 No Hit GCTCCAATACCTAACCAAAGAGCTACTGCAGTACCGATTAAAAAAACGGT 5 0.125 No Hit CCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAA 5 0.125 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 5 0.125 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 5 0.125 No Hit GGGAATTCGTAGATCCTCCAGACGTAGAGCACGTAGGGCTTTGAAACCAA 5 0.125 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 5 0.125 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 5 0.125 No Hit CCCAGGAACAGGCTCGATGTGATAGCATCGTCCTTTGTAACGATCAAGAC 5 0.125 No Hit ATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAGGAC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0125 0.0 0.0 0.0 0.0 134-135 0.037500000000000006 0.0 0.0 0.0 0.0 136-137 0.075 0.0 0.0 0.0 0.0 138 0.1 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTTTT 10 0.006973645 144.0 9 CCCATAT 10 0.006973645 144.0 3 ATAACAA 10 0.006973645 144.0 8 ACCCATA 10 0.006973645 144.0 2 GACCCAT 10 0.006973645 144.0 1 CTTCTCG 10 0.006973645 144.0 1 CGATCTA 10 0.006973645 144.0 4 CTTTTCT 20 3.687869E-4 108.0 6 TTTCTTC 25 8.956223E-4 86.399994 8 TTTTCTT 25 8.956223E-4 86.399994 7 TCTTTTC 30 0.0018473949 72.0 5 TTTCTTT 35 0.0034045284 61.714283 3 TTCTTTT 35 0.0034045284 61.714283 4 TTCTTCA 35 0.0034045284 61.714283 9 GCTTTCT 35 0.0034045284 61.714283 1 CTTTCTT 40 0.005777437 54.0 2 TAGCGGG 20 0.006139246 28.8 55-59 TAGCGGA 20 0.006139246 28.8 30-34 AAATTCT 20 0.006139246 28.8 15-19 TTCAAAA 25 5.183459E-4 28.8 10-14 >>END_MODULE ERR11006574 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006574_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.184 37.0 37.0 37.0 37.0 37.0 2 36.096 37.0 37.0 37.0 37.0 37.0 3 36.2015 37.0 37.0 37.0 37.0 37.0 4 36.236 37.0 37.0 37.0 37.0 37.0 5 36.05 37.0 37.0 37.0 37.0 37.0 6 36.1855 37.0 37.0 37.0 37.0 37.0 7 36.1 37.0 37.0 37.0 37.0 37.0 8 36.279 37.0 37.0 37.0 37.0 37.0 9 36.223 37.0 37.0 37.0 37.0 37.0 10-14 36.3132 37.0 37.0 37.0 37.0 37.0 15-19 36.2606 37.0 37.0 37.0 37.0 37.0 20-24 36.257 37.0 37.0 37.0 37.0 37.0 25-29 36.214600000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.1518 37.0 37.0 37.0 37.0 37.0 35-39 36.143100000000004 37.0 37.0 37.0 37.0 37.0 40-44 36.150099999999995 37.0 37.0 37.0 37.0 37.0 45-49 36.0623 37.0 37.0 37.0 37.0 37.0 50-54 36.0433 37.0 37.0 37.0 37.0 37.0 55-59 35.9925 37.0 37.0 37.0 37.0 37.0 60-64 35.9402 37.0 37.0 37.0 37.0 37.0 65-69 35.8809 37.0 37.0 37.0 37.0 37.0 70-74 35.9127 37.0 37.0 37.0 37.0 37.0 75-79 35.8667 37.0 37.0 37.0 37.0 37.0 80-84 35.8467 37.0 37.0 37.0 37.0 37.0 85-89 35.7569 37.0 37.0 37.0 37.0 37.0 90-94 35.668 37.0 37.0 37.0 37.0 37.0 95-99 35.5795 37.0 37.0 37.0 37.0 37.0 100-104 35.5155 37.0 37.0 37.0 37.0 37.0 105-109 35.502199999999995 37.0 37.0 37.0 37.0 37.0 110-114 35.4678 37.0 37.0 37.0 37.0 37.0 115-119 35.3009 37.0 37.0 37.0 29.8 37.0 120-124 35.4557 37.0 37.0 37.0 34.6 37.0 125-129 35.350100000000005 37.0 37.0 37.0 32.2 37.0 130-134 35.2188 37.0 37.0 37.0 27.4 37.0 135-139 35.1982 37.0 37.0 37.0 25.0 37.0 140-144 34.9956 37.0 37.0 37.0 25.0 37.0 145-149 35.012600000000006 37.0 37.0 37.0 25.0 37.0 150 34.8505 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 2.0 23 4.0 24 4.0 25 7.0 26 11.0 27 9.0 28 12.0 29 18.0 30 31.0 31 45.0 32 65.0 33 93.0 34 250.0 35 944.0 36 2428.0 37 76.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.999999999999996 24.675 18.05 28.275 2 26.1 25.174999999999997 32.5 16.225 3 20.825 26.724999999999998 32.35 20.1 4 24.525 27.675 26.950000000000003 20.849999999999998 5 22.85 29.5 28.275 19.375 6 20.424999999999997 34.225 27.450000000000003 17.9 7 21.025 20.0 39.25 19.725 8 22.05 22.7 30.075000000000003 25.174999999999997 9 22.2 21.075 34.225 22.5 10-14 23.955000000000002 26.534999999999997 28.389999999999997 21.12 15-19 24.104999999999997 25.474999999999998 29.830000000000002 20.59 20-24 24.740000000000002 24.21 29.665000000000003 21.385 25-29 24.665 24.535 29.675 21.125 30-34 24.265 24.32 30.294999999999998 21.12 35-39 24.145 24.925 29.665000000000003 21.265 40-44 23.575 25.564999999999998 29.465000000000003 21.395 45-49 22.555 27.575 27.99 21.88 50-54 22.645 26.75 28.33 22.275 55-59 23.669999999999998 24.955 28.59 22.785 60-64 22.75 25.130000000000003 29.59 22.53 65-69 23.535 25.775 28.575 22.115000000000002 70-74 24.529999999999998 25.395 28.835 21.240000000000002 75-79 24.965 24.215 29.49 21.33 80-84 24.7 25.775 28.68 20.845 85-89 24.610000000000003 26.205000000000002 27.075 22.11 90-94 24.08 25.145 28.355000000000004 22.42 95-99 23.905 25.069999999999997 29.244999999999997 21.78 100-104 25.019999999999996 24.45 29.215000000000003 21.315 105-109 24.83 23.974999999999998 28.96 22.235 110-114 24.535 24.654999999999998 28.804999999999996 22.005 115-119 24.455 25.275 28.694999999999997 21.575 120-124 24.55 25.455 28.22 21.775 125-129 23.65 25.97 28.38 22.0 130-134 23.565 26.200000000000003 27.794999999999998 22.439999999999998 135-139 23.46 25.83 29.044999999999998 21.665 140-144 23.995 24.59 30.0 21.415 145-149 23.395 24.395 30.064999999999998 22.145 150 24.15 23.974999999999998 27.474999999999998 24.4 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 1.5 21 2.0 22 3.5 23 4.0 24 4.5 25 10.5 26 12.5 27 14.0 28 25.0 29 29.5 30 29.5 31 33.0 32 31.5 33 38.0 34 66.5 35 98.5 36 127.0 37 182.0 38 220.5 39 218.5 40 221.0 41 236.0 42 224.5 43 215.0 44 207.5 45 178.5 46 167.5 47 150.0 48 119.0 49 90.5 50 79.0 51 64.0 52 53.0 53 50.0 54 40.0 55 35.0 56 34.0 57 31.0 58 26.0 59 33.0 60 43.0 61 42.5 62 37.0 63 49.0 64 54.0 65 49.0 66 43.0 67 28.0 68 30.0 69 31.0 70 28.0 71 24.5 72 19.0 73 19.0 74 22.5 75 19.0 76 16.0 77 15.0 78 7.0 79 4.0 80 3.0 81 3.0 82 3.0 83 1.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.125 #Duplication Level Percentage of deduplicated Percentage of total 1 81.67521367521367 59.724999999999994 2 12.478632478632479 18.25 3 2.8034188034188037 6.15 4 0.9230769230769231 2.7 5 0.6837606837606838 2.5 6 0.37606837606837606 1.6500000000000001 7 0.3076923076923077 1.575 8 0.27350427350427353 1.6 9 0.06837606837606838 0.44999999999999996 >10 0.37606837606837606 3.975 >50 0.03418803418803419 1.425 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 57 1.425 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 24 0.6 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 19 0.475 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 17 0.42500000000000004 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 16 0.4 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 14 0.35000000000000003 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 13 0.325 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 13 0.325 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 12 0.3 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 11 0.27499999999999997 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 10 0.25 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 10 0.25 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 9 0.22499999999999998 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 9 0.22499999999999998 No Hit ATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGC 8 0.2 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 8 0.2 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 8 0.2 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 8 0.2 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 8 0.2 No Hit CAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTA 8 0.2 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 8 0.2 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 8 0.2 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 7 0.17500000000000002 No Hit ATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCAC 7 0.17500000000000002 No Hit TATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACC 7 0.17500000000000002 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 7 0.17500000000000002 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 7 0.17500000000000002 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 7 0.17500000000000002 No Hit TGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACT 7 0.17500000000000002 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 7 0.17500000000000002 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 7 0.17500000000000002 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 6 0.15 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 6 0.15 No Hit CAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGT 6 0.15 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 6 0.15 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 6 0.15 No Hit GAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTC 6 0.15 No Hit GTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTC 6 0.15 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 6 0.15 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 6 0.15 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 6 0.15 No Hit GTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGG 6 0.15 No Hit GTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTC 5 0.125 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 5 0.125 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 5 0.125 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 5 0.125 No Hit ATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCT 5 0.125 No Hit TGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACT 5 0.125 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 5 0.125 No Hit CTTAATTGTTGAGAGGGTTGCCCAAACGCGGTATATAAGGCATACCCAGT 5 0.125 No Hit AATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTAT 5 0.125 No Hit GCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGC 5 0.125 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 5 0.125 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 5 0.125 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 5 0.125 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 5 0.125 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 5 0.125 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 5 0.125 No Hit CTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTAC 5 0.125 No Hit TACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGG 5 0.125 No Hit CTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATG 5 0.125 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0125 0.0 0.0 0.0 0.0 134-135 0.037500000000000006 0.0 0.0 0.0 0.0 136-137 0.075 0.0 0.0 0.0 0.0 138 0.1 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 3380732 spots for ERR11006574.sra Written 3380732 spots for ERR11006574.sra Read 3380732 spots for ERR11006574.sra Written 3380732 spots for ERR11006574.sra Read 3380732 spots for ERR11006574.sra Written 3380732 spots for ERR11006574.sra Read 3380733 spots for ERR11006574.sra Written 3380733 spots for ERR11006574.sra Read 3380732 spots for ERR11006574.sra Written 3380732 spots for ERR11006574.sra Read 3380732 spots for ERR11006574.sra Written 3380732 spots for ERR11006574.sra Read 3380732 spots for ERR11006574.sra Written 3380732 spots for ERR11006574.sra Read 3380732 spots for ERR11006574.sra Written 3380732 spots for ERR11006574.sra Read 3380732 spots for ERR11006574.sra Written 3380732 spots for ERR11006574.sra Read 3380732 spots for ERR11006574.sra Written 3380732 spots for ERR11006574.sra Read 3380732 spots for ERR11006574.sra Written 3380732 spots for ERR11006574.sra Read 3380732 spots for ERR11006574.sra Written 3380732 spots for ERR11006574.sra Read 3380732 spots for ERR11006574.sra Written 3380732 spots for ERR11006574.sra Read 3380732 spots for ERR11006574.sra Written 3380732 spots for ERR11006574.sra Read 3380732 spots for ERR11006574.sra Written 3380732 spots for ERR11006574.sra Read 3380732 spots for ERR11006574.sra Written 3380732 spots for ERR11006574.sra Read 3380732 spots for ERR11006574.sra Written 3380732 spots for ERR11006574.sra Read 3380732 spots for ERR11006574.sra Written 3380732 spots for ERR11006574.sra Read 3380732 spots for ERR11006574.sra Written 3380732 spots for ERR11006574.sra Read 3380732 spots for ERR11006574.sra Written 3380732 spots for ERR11006574.sra SRR ids: ['ERR11006574.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_w6au6km1 ERR11006574.sra spots: 67614641 blocks: [[1, 3380732], [3380733, 6761464], [6761465, 10142196], [10142197, 13522928], [13522929, 16903660], [16903661, 20284392], [20284393, 23665124], [23665125, 27045856], [27045857, 30426588], [30426589, 33807320], [33807321, 37188052], [37188053, 40568784], [40568785, 43949516], [43949517, 47330248], [47330249, 50710980], [50710981, 54091712], [54091713, 57472444], [57472445, 60853176], [60853177, 64233908], [64233909, 67614641]] ERR11006574 file size 24848130 ERR11006574 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006574 ERR11006574_1.fastq ERR11006574_2.fastq Input file: ERR11006574_1.fastq Paired file: ERR11006574_2.fastq trimmed: ERR11006574-trimmed-pair1.fastq, ERR11006574-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 17:48:00 2024 >> started Fri Dec 6 17:49:20 2024 >> done (79.233s) 67614641 read pairs processed; of these: 283 ( 0.00%) short read pairs filtered out after trimming by size control 829 ( 0.00%) empty read pairs filtered out after trimming by size control 67613529 (100.00%) read pairs available; of these: 253084 ( 0.37%) trimmed read pairs available after processing 67360445 (99.63%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 16 0.00% 19 13 0.00% 20 12 0.00% 21 31 0.00% 22 44 0.00% 23 22 0.00% 24 15 0.00% 25 26 0.00% 26 20 0.00% 27 14 0.00% 28 29 0.00% 29 27 0.00% 30 17 0.00% 31 65 0.00% 32 32 0.00% 33 35 0.00% 34 34 0.00% 35 39 0.00% 36 29 0.00% 37 34 0.00% 38 49 0.00% 39 57 0.00% 40 62 0.00% 41 40 0.00% 42 46 0.00% 43 55 0.00% 44 59 0.00% 45 52 0.00% 46 66 0.00% 47 39 0.00% 48 63 0.00% 49 64 0.00% 50 71 0.00% 51 62 0.00% 52 69 0.00% 53 68 0.00% 54 82 0.00% 55 107 0.00% 56 96 0.00% 57 116 0.00% 58 102 0.00% 59 107 0.00% 60 106 0.00% 61 145 0.00% 62 122 0.00% 63 145 0.00% 64 130 0.00% 65 149 0.00% 66 170 0.00% 67 162 0.00% 68 162 0.00% 69 193 0.00% 70 161 0.00% 71 191 0.00% 72 226 0.00% 73 208 0.00% 74 247 0.00% 75 240 0.00% 76 308 0.00% 77 258 0.00% 78 317 0.00% 79 332 0.00% 80 342 0.00% 81 350 0.00% 82 405 0.00% 83 447 0.00% 84 454 0.00% 85 490 0.00% 86 517 0.00% 87 572 0.00% 88 550 0.00% 89 648 0.00% 90 661 0.00% 91 681 0.00% 92 699 0.00% 93 781 0.00% 94 720 0.00% 95 884 0.00% 96 886 0.00% 97 1053 0.00% 98 1087 0.00% 99 1073 0.00% 100 1201 0.00% 101 1122 0.00% 102 1209 0.00% 103 1318 0.00% 104 1421 0.00% 105 1480 0.00% 106 1640 0.00% 107 1667 0.00% 108 1723 0.00% 109 1790 0.00% 110 2099 0.00% 111 2091 0.00% 112 2296 0.00% 113 2270 0.00% 114 2375 0.00% 115 2431 0.00% 116 2800 0.00% 117 2857 0.00% 118 2759 0.00% 119 2976 0.00% 120 3278 0.00% 121 3433 0.01% 122 3787 0.01% 123 3944 0.01% 124 3991 0.01% 125 4569 0.01% 126 4278 0.01% 127 4249 0.01% 128 4594 0.01% 129 5023 0.01% 130 5253 0.01% 131 5596 0.01% 132 5821 0.01% 133 5399 0.01% 134 5715 0.01% 135 5780 0.01% 136 6326 0.01% 137 6219 0.01% 138 6384 0.01% 139 6995 0.01% 140 7234 0.01% 141 7613 0.01% 142 8233 0.01% 143 8686 0.01% 144 9035 0.01% 145 9293 0.01% 146 11114 0.02% 147 10518 0.02% 148 10539 0.02% 149 11302 0.02% 150 67360445 99.63% 67613529 reads passed initial QC criterion=sequence-density sequence-density=0.36 sequence-density-rank=1 fanout-score=1.97 fanout-score-rank=32 prefix-density=0.33 prefix-fanout=2.0 sequence=TCTAATTCAAAA criterion=fanout-score sequence-density=0.01 sequence-density-rank=42 fanout-score=105.56 fanout-score-rank=1 prefix-density=0.31 prefix-fanout=2.1 sequence=AAAAACAGTAGAAGTAGAACAGGTATAAATAAGAAAATCTTAGTTAAGAGGGTTCATGTAAAGAACAGGTTCTAAATCACGATCGATTCCCTTTTCAAAACCTGCTGCAGCAGCTCGGGCTCTTCCTGCATGCCACAAATGGCCCACAAAAAAGAAGAATCCTAGAACAAAATGAGAAGTCGATAACCAACTTCTAGGAGAGACATAATTAACTGCATTGATCTCGGTAGCTACGCCACCCACGGAATTTAAAGAGCCTAAAGGAGCATGGGTCATATATTCCGCTGAACGTCGTTCTTGCCAAGGTTGTATGTCTTTTTTCAACCTACTCAAGTCCAAACCGTTGGGCCCCCTTAGAGGTTCTAACCATGGAGCACGGAGGTCCCAAAAACGCATAGTTTCCCCTCCAAAGATAACCTCTCCCGTTGGGGAACGCATTAGATATTTACCTAAACCTGTGGGTCCTTGAGCAGATCCCACATTAGCTCCAAGACGCTGGTCTCTAACTAGA criterion=sequence-density sequence-density=0.60 sequence-density-rank=1 fanout-score=4.80 fanout-score-rank=20 prefix-density=0.97 prefix-fanout=3.0 sequence=TGGGGAAGAGGA criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=41.19 fanout-score-rank=1 prefix-density=0.15 prefix-fanout=2.2 sequence=ATTGATTTTTGGATTTCGATCGTATCTAAGATTCTTTTTTATCTGGGTTGCTAACTCAATGGTAGAGTACTCGGCTTTTAAGTGCGACTATGATCTTTTACACATTTGGATGAAGCAACAAATTCGTCCAGACTCTTGGTAGAGTCTAGAAGACCACGACTGATCCTCAAGGGTAATGAATGGAAAAAAAAGCATGTCGTAATAAAATTCATTTCTTTT ERR11006574 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 17:49:55 Started mapping on | Dec 06 17:49:55 Finished on | Dec 06 17:56:51 Mapping speed, Million of reads per hour | 585.12 Number of input reads | 67613529 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 49484181 Uniquely mapped reads % | 73.19% Average mapped length | 298.61 Number of splices: Total | 19220751 Number of splices: Annotated (sjdb) | 17902273 Number of splices: GT/AG | 18740474 Number of splices: GC/AG | 233487 Number of splices: AT/AC | 24180 Number of splices: Non-canonical | 222610 Mismatch rate per base, % | 0.30% Deletion rate per base | 0.01% Deletion average length | 1.83 Insertion rate per base | 0.01% Insertion average length | 1.97 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 16033141 % of reads mapped to multiple loci | 23.71% Number of reads mapped to too many loci | 9143 % of reads mapped to too many loci | 0.01% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.89% % of reads unmapped: other | 0.20% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2096207 2096207 2096207 N_multimapping 16033141 16033141 16033141 N_noFeature 11356662 44772943 14553886 N_ambiguous 2706379 86080 1232018 UnstrandedReadsAssigned:35421140 PositiveStrandReadsAssigned:4625158 NegativeStrandReadsAssigned:33698277 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006574 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006574-trimmed-pair1.fastq ERR11006574-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 67,613,529 reads, 41,132,574 reads pseudoaligned [quant] estimated average fragment length: 308.271 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,116 rounds 52973 ERR11006574.ke.tsv 35125 ERR11006574.se.tsv 88098 total ==> ERR11006574.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 629.123 0 0 PNS24247 1044 736.729 23.9466 0.749418 PNS24249 1928 1620.73 132.379 1.88321 PNS24246 1044 736.729 23.9466 0.749418 PNS24248 1044 736.729 23.9466 0.749418 PNS24244 1471 1163.73 39.7808 0.788152 PNS24243 293 43.7762 0 0 KQK14069 1603 1295.73 3772.94 67.1358 KQK14071 474 171.562 37.1867 4.99753 ==> ERR11006574.se.tsv <== BRADI_1g14170v3 4086 BRADI_1g53295v3 93 BRADI_1g59795v3 247 BRADI_1g07683v3 0 BRADI_1g00485v3 8 BRADI_1g20270v3 582 BRADI_1g74790v3 242 BRADI_1g09890v3 1 BRADI_1g77505v3 244 BRADI_1g48960v3 0 ERR11006574 completed mapping pipeline successfully