Starting /dee2/code/volunteer_pipeline.sh ERR11006575 current disk space = 1550743482368 free memory = 1345326924 ERR11006575 SRAfilesize 80133d1ead92b9a9cc4e2012257e2e12 ERR11006575.sra ERR11006575.sra file validated ERR11006575 is paired end ERR11006575 is conventional basespace ERR11006575 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006575_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.11025 37.0 37.0 37.0 37.0 37.0 2 36.27675 37.0 37.0 37.0 37.0 37.0 3 36.379 37.0 37.0 37.0 37.0 37.0 4 36.334 37.0 37.0 37.0 37.0 37.0 5 36.3925 37.0 37.0 37.0 37.0 37.0 6 36.4825 37.0 37.0 37.0 37.0 37.0 7 36.4455 37.0 37.0 37.0 37.0 37.0 8 36.4625 37.0 37.0 37.0 37.0 37.0 9 36.3725 37.0 37.0 37.0 37.0 37.0 10-14 36.4382 37.0 37.0 37.0 37.0 37.0 15-19 36.3679 37.0 37.0 37.0 37.0 37.0 20-24 36.364599999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.327999999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.23309999999999 37.0 37.0 37.0 37.0 37.0 35-39 36.1887 37.0 37.0 37.0 37.0 37.0 40-44 36.161150000000006 37.0 37.0 37.0 37.0 37.0 45-49 36.1601 37.0 37.0 37.0 37.0 37.0 50-54 36.1324 37.0 37.0 37.0 37.0 37.0 55-59 36.1581 37.0 37.0 37.0 37.0 37.0 60-64 36.0068 37.0 37.0 37.0 37.0 37.0 65-69 36.099599999999995 37.0 37.0 37.0 37.0 37.0 70-74 36.082300000000004 37.0 37.0 37.0 37.0 37.0 75-79 36.143899999999995 37.0 37.0 37.0 37.0 37.0 80-84 36.033 37.0 37.0 37.0 37.0 37.0 85-89 35.972500000000004 37.0 37.0 37.0 37.0 37.0 90-94 35.87949999999999 37.0 37.0 37.0 37.0 37.0 95-99 35.9191 37.0 37.0 37.0 37.0 37.0 100-104 35.925799999999995 37.0 37.0 37.0 37.0 37.0 105-109 35.853899999999996 37.0 37.0 37.0 37.0 37.0 110-114 35.7261 37.0 37.0 37.0 37.0 37.0 115-119 35.798700000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.6312 37.0 37.0 37.0 37.0 37.0 125-129 35.7505 37.0 37.0 37.0 37.0 37.0 130-134 35.5755 37.0 37.0 37.0 37.0 37.0 135-139 35.458600000000004 37.0 37.0 37.0 34.6 37.0 140-144 35.6628 37.0 37.0 37.0 37.0 37.0 145-149 35.3976 37.0 37.0 37.0 34.6 37.0 150 35.4615 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 1.0 24 1.0 25 3.0 26 12.0 27 14.0 28 20.0 29 27.0 30 42.0 31 41.0 32 67.0 33 86.0 34 135.0 35 389.0 36 3062.0 37 100.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.53445251816587 11.851666249060386 14.282134803307441 36.3317464294663 2 26.806701675418854 10.202550637659416 26.6816704176044 36.30907726931733 3 22.325 12.1 28.849999999999998 36.725 4 27.400000000000002 14.274999999999999 25.124999999999996 33.2 5 29.049999999999997 17.65 27.525 25.775 6 24.474999999999998 27.35 23.35 24.825 7 19.025 26.575 34.65 19.75 8 15.950000000000001 28.625 34.2 21.224999999999998 9 17.2 26.025 35.875 20.9 10-14 19.875 31.55 27.495000000000005 21.08 15-19 19.735 33.535 25.25 21.48 20-24 18.45 30.099999999999998 28.585 22.865 25-29 21.08 31.655 26.179999999999996 21.085 30-34 23.505000000000003 30.54 25.155 20.8 35-39 22.185 31.6 25.775 20.44 40-44 19.784623090408214 30.15276734284999 26.321061858251944 23.741547708489858 45-49 19.73 29.360000000000003 27.72 23.189999999999998 50-54 20.665 31.619999999999997 26.075 21.64 55-59 22.115000000000002 29.985 23.66 24.240000000000002 60-64 19.830000000000002 31.355 25.31 23.505000000000003 65-69 20.25 30.36 25.7 23.69 70-74 22.35 29.385 23.275000000000002 24.990000000000002 75-79 21.985 28.775000000000002 26.265 22.975 80-84 21.67 29.525000000000002 25.405 23.400000000000002 85-89 24.474999999999998 28.615000000000002 24.9 22.009999999999998 90-94 21.705 30.39 26.540000000000003 21.365000000000002 95-99 23.630000000000003 29.895 23.355 23.119999999999997 100-104 22.015 30.7 24.32 22.965 105-109 21.36 28.62 26.985 23.035 110-114 23.810000000000002 28.29 24.865000000000002 23.035 115-119 20.455000000000002 29.985 25.365 24.195 120-124 20.885 29.415000000000003 24.92 24.779999999999998 125-129 21.04 31.055 22.745 25.16 130-134 23.205000000000002 30.080000000000002 24.745 21.97 135-139 23.283896181982165 28.91071249624211 23.820022046297222 23.985369275478504 140-144 24.745 28.610000000000003 26.105 20.54 145-149 22.89 30.665 25.35 21.095 150 23.05 27.575 28.299999999999997 21.075 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.5 20 1.0 21 0.5 22 2.5 23 3.5 24 7.0 25 9.0 26 7.5 27 7.0 28 9.5 29 13.0 30 18.5 31 23.0 32 30.0 33 35.0 34 40.0 35 54.0 36 91.0 37 162.5 38 269.5 39 296.0 40 263.0 41 282.0 42 274.5 43 255.5 44 234.5 45 213.0 46 198.5 47 159.0 48 115.0 49 84.5 50 87.0 51 73.5 52 46.5 53 42.0 54 32.5 55 30.5 56 33.5 57 30.5 58 24.0 59 25.5 60 30.5 61 27.0 62 20.0 63 20.0 64 37.0 65 50.5 66 35.5 67 23.5 68 22.0 69 16.5 70 16.0 71 20.5 72 18.5 73 12.0 74 10.5 75 11.0 76 9.0 77 8.5 78 7.0 79 4.5 80 5.5 81 3.5 82 1.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22499999999999998 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.17500000000000002 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.21 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.85 #Duplication Level Percentage of deduplicated Percentage of total 1 82.76385725132877 54.50000000000001 2 10.858010630220196 14.299999999999999 3 2.5056947608200453 4.95 4 1.0250569476082005 2.7 5 0.3796507213363705 1.25 6 0.683371298405467 2.7 7 0.22779043280182232 1.05 8 0.3416856492027335 1.7999999999999998 9 0.11389521640091116 0.675 >10 1.0630220197418374 13.0 >50 0.0 0.0 >100 0.037965072133637055 3.075 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 123 3.075 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 39 0.975 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 38 0.95 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 36 0.8999999999999999 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 30 0.75 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 28 0.7000000000000001 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 25 0.625 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 25 0.625 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 24 0.6 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 21 0.525 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 19 0.475 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 18 0.44999999999999996 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 16 0.4 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 16 0.4 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 15 0.375 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 15 0.375 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 14 0.35000000000000003 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 14 0.35000000000000003 No Hit AACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAG 14 0.35000000000000003 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 14 0.35000000000000003 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 13 0.325 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 12 0.3 No Hit CCCAGGAACAGGCTCGATGTGATAGCATCGTCCTTTGTAACGATCAAGAC 12 0.3 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 11 0.27499999999999997 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 11 0.27499999999999997 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 10 0.25 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 10 0.25 No Hit TGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCT 10 0.25 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 10 0.25 No Hit ACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 9 0.22499999999999998 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 9 0.22499999999999998 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 9 0.22499999999999998 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 8 0.2 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 8 0.2 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 8 0.2 No Hit GGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAG 8 0.2 No Hit CGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAAC 8 0.2 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 8 0.2 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 8 0.2 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 8 0.2 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 8 0.2 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 7 0.17500000000000002 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 7 0.17500000000000002 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 7 0.17500000000000002 No Hit CCCCAGGAACAGGCTCGATGTGATAGCATCGTCCTTTGTAACGATCAAGA 7 0.17500000000000002 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 7 0.17500000000000002 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 7 0.17500000000000002 No Hit CCCTCTTCAAATAGATCTAATGGATAAGCTACATAACAGATCCATTGACT 6 0.15 No Hit GCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTAAG 6 0.15 No Hit TGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATT 6 0.15 No Hit CCCCAGTTAAGTAGTCATGCATTACAATAGGAACACCTAATTCTCTCGCA 6 0.15 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 6 0.15 No Hit ATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCT 6 0.15 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 6 0.15 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 6 0.15 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 6 0.15 No Hit GGCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGAT 6 0.15 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 6 0.15 No Hit CAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGA 6 0.15 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 6 0.15 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 6 0.15 No Hit CACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAAT 6 0.15 No Hit GCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTC 6 0.15 No Hit AGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGG 6 0.15 No Hit CCCCCAGTTAAGTAGTCATGCATTACAATAGGAACACCTAATTCTCTCGC 6 0.15 No Hit GGGGGAATTCGTAGATCCTCCAGACGTAGAGCACGTAGGGCTTTGAAACC 5 0.125 No Hit TAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTA 5 0.125 No Hit TCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGT 5 0.125 No Hit GCCCTTCGTTACGAGCTTGTACACAGGCTTCTAAAGCCACTCGATTAGCT 5 0.125 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 5 0.125 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 5 0.125 No Hit GAGCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTA 5 0.125 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 5 0.125 No Hit GGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 5 0.125 No Hit GGGGAATTCGTAGATCCTCCAGACGTAGAGCACGTAGGGCTTTGAAACCA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.025 0.0 0.0 0.0 0.0 102-103 0.025 0.0 0.0 0.0 0.0 104-105 0.025 0.0 0.0 0.0 0.0 106-107 0.025 0.0 0.0 0.0 0.0 108-109 0.025 0.0 0.0 0.0 0.0 110-111 0.025 0.0 0.0 0.0 0.0 112-113 0.025 0.0 0.0 0.0 0.0 114-115 0.025 0.0 0.0 0.0 0.0 116-117 0.025 0.0 0.0 0.0 0.0 118-119 0.05 0.0 0.0 0.0 0.0 120-121 0.05 0.0 0.0 0.0 0.0 122-123 0.05 0.0 0.0 0.0 0.0 124-125 0.05 0.0 0.0 0.0 0.0 126-127 0.05 0.0 0.0 0.0 0.0 128-129 0.0875 0.0 0.0 0.0 0.0 130-131 0.125 0.0 0.0 0.0 0.0 132-133 0.15 0.0 0.0 0.0 0.0 134-135 0.15 0.0 0.0 0.0 0.0 136-137 0.175 0.0 0.0 0.0 0.0 138 0.2 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTTCT 30 9.508585E-8 120.0 1 CTTTTCT 35 2.3845314E-7 102.85714 6 TTTCTTT 40 5.2840005E-7 90.0 3 TTTTCTT 40 5.2840005E-7 90.0 7 CTTTCTT 40 5.2840005E-7 90.0 2 TTCTTTT 45 1.0653384E-6 80.0 4 TCTTTTC 45 1.0653384E-6 80.0 5 TTGTGCT 30 0.0018473949 72.0 2 GTTGTGC 30 0.0018473949 72.0 1 TTTCTTC 55 3.5124358E-6 65.454544 8 TTCTTCA 55 3.5124358E-6 65.454544 9 CTCTGCC 35 0.0034045284 61.714283 7 TCTGCCT 35 0.0034045284 61.714283 8 TGCTCTG 35 0.0034045284 61.714283 5 GCTCTGC 35 0.0034045284 61.714283 6 TGTGCTC 35 0.0034045284 61.714283 3 GTGCTCT 35 0.0034045284 61.714283 4 CTGCCTG 40 0.005777437 54.0 9 TAGCGGA 35 3.7333357E-6 28.8 30-34 GCGGGAA 35 3.7333357E-6 28.8 55-59 >>END_MODULE ERR11006575 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006575_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.76025 37.0 37.0 37.0 25.0 37.0 2 35.7485 37.0 37.0 37.0 37.0 37.0 3 35.798 37.0 37.0 37.0 37.0 37.0 4 35.8915 37.0 37.0 37.0 37.0 37.0 5 35.8445 37.0 37.0 37.0 37.0 37.0 6 35.8215 37.0 37.0 37.0 37.0 37.0 7 35.9255 37.0 37.0 37.0 37.0 37.0 8 36.0505 37.0 37.0 37.0 37.0 37.0 9 35.9485 37.0 37.0 37.0 37.0 37.0 10-14 35.9797 37.0 37.0 37.0 37.0 37.0 15-19 35.925999999999995 37.0 37.0 37.0 37.0 37.0 20-24 35.866600000000005 37.0 37.0 37.0 37.0 37.0 25-29 35.8095 37.0 37.0 37.0 37.0 37.0 30-34 35.8082 37.0 37.0 37.0 37.0 37.0 35-39 35.7293 37.0 37.0 37.0 37.0 37.0 40-44 35.754 37.0 37.0 37.0 37.0 37.0 45-49 35.738200000000006 37.0 37.0 37.0 37.0 37.0 50-54 35.7362 37.0 37.0 37.0 37.0 37.0 55-59 35.6096 37.0 37.0 37.0 37.0 37.0 60-64 35.55839999999999 37.0 37.0 37.0 34.6 37.0 65-69 35.5193 37.0 37.0 37.0 37.0 37.0 70-74 35.474599999999995 37.0 37.0 37.0 37.0 37.0 75-79 35.5535 37.0 37.0 37.0 37.0 37.0 80-84 35.4601 37.0 37.0 37.0 34.6 37.0 85-89 35.428399999999996 37.0 37.0 37.0 37.0 37.0 90-94 35.31160000000001 37.0 37.0 37.0 32.2 37.0 95-99 35.2903 37.0 37.0 37.0 32.2 37.0 100-104 35.0871 37.0 37.0 37.0 25.0 37.0 105-109 35.117000000000004 37.0 37.0 37.0 27.4 37.0 110-114 34.97 37.0 37.0 37.0 25.0 37.0 115-119 34.9237 37.0 37.0 37.0 25.0 37.0 120-124 35.0322 37.0 37.0 37.0 25.0 37.0 125-129 34.9209 37.0 37.0 37.0 25.0 37.0 130-134 34.7822 37.0 37.0 37.0 25.0 37.0 135-139 34.650999999999996 37.0 37.0 37.0 25.0 37.0 140-144 34.634100000000004 37.0 37.0 37.0 25.0 37.0 145-149 34.4288 37.0 37.0 37.0 25.0 37.0 150 34.2795 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 0.0 17 1.0 18 0.0 19 0.0 20 0.0 21 2.0 22 7.0 23 5.0 24 7.0 25 17.0 26 21.0 27 26.0 28 28.0 29 26.0 30 36.0 31 41.0 32 121.0 33 145.0 34 347.0 35 1138.0 36 1978.0 37 53.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.637890927888343 26.053243732230552 17.653140346342724 27.65572499353838 2 25.6 24.6 33.925 15.875 3 19.925 27.700000000000003 34.449999999999996 17.925 4 22.45 30.049999999999997 28.449999999999996 19.05 5 23.474999999999998 29.075 28.849999999999998 18.6 6 20.45 33.525 27.875 18.15 7 19.45 22.15 40.425 17.974999999999998 8 20.674999999999997 23.275000000000002 30.9 25.15 9 24.0 19.950000000000003 34.375 21.675 10-14 23.97 27.3 28.549999999999997 20.18 15-19 23.66 26.240000000000002 29.92 20.18 20-24 24.395 24.575 29.99 21.04 25-29 24.18 24.57 30.165 21.085 30-34 24.245 24.735 30.259999999999998 20.76 35-39 23.54 25.165 30.325000000000003 20.97 40-44 23.169999999999998 26.31 29.38 21.14 45-49 22.68 26.855 29.335 21.13 50-54 21.845 26.35 29.12 22.685 55-59 22.725 25.55 28.92 22.805 60-64 22.46 25.979999999999997 29.110000000000003 22.45 65-69 22.975 25.455 29.849999999999998 21.72 70-74 22.919999999999998 25.535000000000004 29.945 21.6 75-79 24.085 25.145 29.56 21.21 80-84 24.485 24.895 29.335 21.285 85-89 24.195 25.674999999999997 28.02 22.11 90-94 23.56 25.47 29.17 21.8 95-99 23.46 25.005 29.580000000000002 21.955 100-104 24.3 24.275 30.240000000000002 21.185000000000002 105-109 25.0 23.119999999999997 30.075000000000003 21.805 110-114 24.03 23.765 30.42 21.785 115-119 23.915 24.42 29.73 21.935 120-124 23.885 24.86 28.375 22.88 125-129 23.135 25.795 28.955 22.115000000000002 130-134 23.345 26.0 28.939999999999998 21.715 135-139 23.465 26.445 29.115000000000002 20.974999999999998 140-144 23.75 25.305 30.345 20.599999999999998 145-149 22.745 24.26 31.185000000000002 21.81 150 23.5 24.6 29.5 22.400000000000002 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 1.5 20 2.5 21 1.0 22 3.0 23 5.5 24 5.5 25 7.0 26 9.5 27 14.5 28 28.0 29 29.5 30 25.5 31 35.0 32 36.0 33 40.5 34 69.0 35 99.0 36 122.5 37 197.0 38 236.5 39 212.0 40 248.0 41 274.0 42 229.5 43 212.5 44 223.0 45 202.0 46 178.0 47 154.5 48 129.0 49 97.0 50 68.5 51 49.0 52 41.0 53 45.5 54 41.0 55 33.0 56 26.0 57 26.5 58 28.5 59 26.5 60 26.5 61 30.0 62 26.5 63 34.5 64 46.0 65 33.0 66 24.0 67 23.5 68 24.5 69 29.5 70 29.0 71 25.0 72 22.5 73 23.0 74 21.0 75 16.5 76 9.5 77 7.0 78 7.5 79 5.0 80 5.0 81 5.0 82 3.5 83 2.0 84 2.5 85 2.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.2750000000000004 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.275 #Duplication Level Percentage of deduplicated Percentage of total 1 83.96451722961447 61.525 2 9.518935516888433 13.950000000000001 3 2.865916069600819 6.3 4 1.3306038894575232 3.9 5 0.7505970658478335 2.75 6 0.511770726714432 2.25 7 0.2388263391334016 1.225 8 0.1364721937905152 0.8 9 0.0341180484476288 0.22499999999999998 >10 0.6482429205049471 7.074999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 45 1.125 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 22 0.5499999999999999 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 17 0.42500000000000004 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 17 0.42500000000000004 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 17 0.42500000000000004 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 15 0.375 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 14 0.35000000000000003 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 14 0.35000000000000003 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 14 0.35000000000000003 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 13 0.325 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 12 0.3 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 11 0.27499999999999997 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 11 0.27499999999999997 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 11 0.27499999999999997 No Hit ATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGC 10 0.25 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 10 0.25 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 10 0.25 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 10 0.25 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 10 0.25 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 9 0.22499999999999998 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 8 0.2 No Hit AATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTAT 8 0.2 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 8 0.2 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 8 0.2 No Hit CCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTAT 7 0.17500000000000002 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 7 0.17500000000000002 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 7 0.17500000000000002 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 7 0.17500000000000002 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 7 0.17500000000000002 No Hit TCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTG 7 0.17500000000000002 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 7 0.17500000000000002 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 6 0.15 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 6 0.15 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 6 0.15 No Hit CAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGT 6 0.15 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 6 0.15 No Hit TTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGA 6 0.15 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 6 0.15 No Hit CTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATT 6 0.15 No Hit AATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGC 6 0.15 No Hit TGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTG 6 0.15 No Hit CTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGG 6 0.15 No Hit TCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGA 6 0.15 No Hit CTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTAC 6 0.15 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 6 0.15 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 6 0.15 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 5 0.125 No Hit TCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAAC 5 0.125 No Hit GTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTC 5 0.125 No Hit ATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATAT 5 0.125 No Hit GGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCG 5 0.125 No Hit CTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTT 5 0.125 No Hit TATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACC 5 0.125 No Hit AATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGT 5 0.125 No Hit GGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTT 5 0.125 No Hit TGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTC 5 0.125 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 5 0.125 No Hit GGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTA 5 0.125 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 5 0.125 No Hit CTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTTATGA 5 0.125 No Hit CAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTA 5 0.125 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 5 0.125 No Hit TAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGA 5 0.125 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 5 0.125 No Hit GAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTT 5 0.125 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 5 0.125 No Hit AGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTAC 5 0.125 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.025 0.0 0.0 0.0 0.0 102-103 0.025 0.0 0.0 0.0 0.0 104-105 0.025 0.0 0.0 0.0 0.0 106-107 0.025 0.0 0.0 0.0 0.0 108-109 0.025 0.0 0.0 0.0 0.0 110-111 0.025 0.0 0.0 0.0 0.0 112-113 0.025 0.0 0.0 0.0 0.0 114-115 0.037500000000000006 0.0 0.0 0.0 0.0 116-117 0.05 0.0 0.0 0.0 0.0 118-119 0.075 0.0 0.0 0.0 0.0 120-121 0.075 0.0 0.0 0.0 0.0 122-123 0.075 0.0 0.0 0.0 0.0 124-125 0.075 0.0 0.0 0.0 0.0 126-127 0.075 0.0 0.0 0.0 0.0 128-129 0.11249999999999999 0.0 0.0 0.0 0.0 130-131 0.15 0.0 0.0 0.0 0.0 132-133 0.175 0.0 0.0 0.0 0.0 134-135 0.175 0.0 0.0 0.0 0.0 136-137 0.2 0.0 0.0 0.0 0.0 138 0.225 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTCCA 10 0.0069772652 143.975 4 ACTTTCC 10 0.0069772652 143.975 3 >>END_MODULE Read 2596434 spots for ERR11006575.sra Written 2596434 spots for ERR11006575.sra Read 2596434 spots for ERR11006575.sra Written 2596434 spots for ERR11006575.sra Read 2596434 spots for ERR11006575.sra Written 2596434 spots for ERR11006575.sra Read 2596434 spots for ERR11006575.sra Written 2596434 spots for ERR11006575.sra Read 2596434 spots for ERR11006575.sra Written 2596434 spots for ERR11006575.sra Read 2596434 spots for ERR11006575.sra Written 2596434 spots for ERR11006575.sra Read 2596434 spots for ERR11006575.sra Written 2596434 spots for ERR11006575.sra Read 2596434 spots for ERR11006575.sra Written 2596434 spots for ERR11006575.sra Read 2596434 spots for ERR11006575.sra Written 2596434 spots for ERR11006575.sra Read 2596450 spots for ERR11006575.sra Written 2596450 spots for ERR11006575.sra Read 2596434 spots for ERR11006575.sra Written 2596434 spots for ERR11006575.sra Read 2596434 spots for ERR11006575.sra Written 2596434 spots for ERR11006575.sra Read 2596434 spots for ERR11006575.sra Written 2596434 spots for ERR11006575.sra Read 2596434 spots for ERR11006575.sra Written 2596434 spots for ERR11006575.sra Read 2596434 spots for ERR11006575.sra Written 2596434 spots for ERR11006575.sra Read 2596434 spots for ERR11006575.sra Written 2596434 spots for ERR11006575.sra Read 2596434 spots for ERR11006575.sra Written 2596434 spots for ERR11006575.sra Read 2596434 spots for ERR11006575.sra Written 2596434 spots for ERR11006575.sra Read 2596434 spots for ERR11006575.sra Written 2596434 spots for ERR11006575.sra Read 2596434 spots for ERR11006575.sra Written 2596434 spots for ERR11006575.sra SRR ids: ['ERR11006575.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_hnnisarj ERR11006575.sra spots: 51928696 blocks: [[1, 2596434], [2596435, 5192868], [5192869, 7789302], [7789303, 10385736], [10385737, 12982170], [12982171, 15578604], [15578605, 18175038], [18175039, 20771472], [20771473, 23367906], [23367907, 25964340], [25964341, 28560774], [28560775, 31157208], [31157209, 33753642], [33753643, 36350076], [36350077, 38946510], [38946511, 41542944], [41542945, 44139378], [44139379, 46735812], [46735813, 49332246], [49332247, 51928696]] ERR11006575 file size 19080715 ERR11006575 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006575 ERR11006575_1.fastq ERR11006575_2.fastq Input file: ERR11006575_1.fastq Paired file: ERR11006575_2.fastq trimmed: ERR11006575-trimmed-pair1.fastq, ERR11006575-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 17:48:46 2024 >> started Fri Dec 6 17:49:53 2024 >> done (67.537s) 51928696 read pairs processed; of these: 562 ( 0.00%) short read pairs filtered out after trimming by size control 984 ( 0.00%) empty read pairs filtered out after trimming by size control 51927150 (100.00%) read pairs available; of these: 234193 ( 0.45%) trimmed read pairs available after processing 51692957 (99.55%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 31 0.00% 19 27 0.00% 20 20 0.00% 21 38 0.00% 22 57 0.00% 23 26 0.00% 24 18 0.00% 25 20 0.00% 26 37 0.00% 27 21 0.00% 28 43 0.00% 29 32 0.00% 30 31 0.00% 31 50 0.00% 32 35 0.00% 33 32 0.00% 34 34 0.00% 35 32 0.00% 36 39 0.00% 37 31 0.00% 38 37 0.00% 39 27 0.00% 40 34 0.00% 41 29 0.00% 42 33 0.00% 43 45 0.00% 44 37 0.00% 45 39 0.00% 46 48 0.00% 47 36 0.00% 48 44 0.00% 49 37 0.00% 50 57 0.00% 51 50 0.00% 52 60 0.00% 53 44 0.00% 54 53 0.00% 55 76 0.00% 56 67 0.00% 57 95 0.00% 58 60 0.00% 59 81 0.00% 60 82 0.00% 61 88 0.00% 62 99 0.00% 63 100 0.00% 64 114 0.00% 65 108 0.00% 66 122 0.00% 67 125 0.00% 68 122 0.00% 69 128 0.00% 70 146 0.00% 71 163 0.00% 72 149 0.00% 73 179 0.00% 74 201 0.00% 75 190 0.00% 76 214 0.00% 77 213 0.00% 78 222 0.00% 79 226 0.00% 80 261 0.00% 81 273 0.00% 82 274 0.00% 83 331 0.00% 84 407 0.00% 85 374 0.00% 86 418 0.00% 87 426 0.00% 88 465 0.00% 89 501 0.00% 90 550 0.00% 91 558 0.00% 92 591 0.00% 93 616 0.00% 94 651 0.00% 95 783 0.00% 96 676 0.00% 97 824 0.00% 98 896 0.00% 99 951 0.00% 100 1020 0.00% 101 988 0.00% 102 1159 0.00% 103 1154 0.00% 104 1260 0.00% 105 1267 0.00% 106 1473 0.00% 107 1519 0.00% 108 1447 0.00% 109 1650 0.00% 110 1763 0.00% 111 1810 0.00% 112 2068 0.00% 113 2000 0.00% 114 2076 0.00% 115 2097 0.00% 116 2466 0.00% 117 2484 0.00% 118 2570 0.00% 119 2746 0.01% 120 3179 0.01% 121 3284 0.01% 122 3658 0.01% 123 3585 0.01% 124 3696 0.01% 125 4369 0.01% 126 4009 0.01% 127 4100 0.01% 128 4179 0.01% 129 4735 0.01% 130 4931 0.01% 131 5220 0.01% 132 5471 0.01% 133 5246 0.01% 134 5483 0.01% 135 5517 0.01% 136 5721 0.01% 137 5874 0.01% 138 6029 0.01% 139 6698 0.01% 140 6488 0.01% 141 7254 0.01% 142 7751 0.01% 143 8296 0.02% 144 8592 0.02% 145 8623 0.02% 146 10479 0.02% 147 9936 0.02% 148 9843 0.02% 149 11370 0.02% 150 51692957 99.55% 51927150 reads passed initial QC criterion=sequence-density sequence-density=0.30 sequence-density-rank=1 fanout-score=1.97 fanout-score-rank=35 prefix-density=0.28 prefix-fanout=2.0 sequence=TCTAATTCAAAA criterion=fanout-score sequence-density=0.01 sequence-density-rank=40 fanout-score=90.55 fanout-score-rank=1 prefix-density=0.33 prefix-fanout=2.0 sequence=AAAAACAGTAGAAGTAGAACAGGTATAAATAAGAAAATCTTAGTTAAGAGGGTTCATGTAAAGAACAGGTTCTAAATCACGATCGATTCCCTTTTCAAAACCTGCTGCAGCAGCTCGGGCTCTTCCTGCATGCCACAAATGGCCCACAAAAAAGAAGAATCCTAGAACAAAATGAGAAGTCGATAACCAACTTCTAGGAGAGACATAATTAACTGCATTGATCTCGGTAGCTACGCCACCCACGGAATTTAAAGAGCCTAAAGGAGCATGGGTCATATATTCCGCTGAACGTCGTTCTTGCCAAGGTTGTATGTCTTTTTTCAACCTACTCAAGTCCAAACCGTTGGGCCCCCTTAGAGGTTCTAACCATGGAGCACGGAGGTCCCAAAAACGCATAGTTTCCCCTCCAAAGATAACCTCTCCCGTTGGGGAACGCATTAGATATTTACCTAAACCTGTGGGTCCTTGAGCAGATCCCACATTAGCTCCAAGACGCTGGTCTCTAACTAGA criterion=sequence-density sequence-density=0.29 sequence-density-rank=1 fanout-score=3.92 fanout-score-rank=28 prefix-density=0.55 prefix-fanout=2.1 sequence=GGGGAAGAGGATCAAGTTGGCCCTTGCGAATAACTTGATGCACTATCTCCCTTCAACCCTTTGAGCGAAATGTAGCAAAAGGAAGCAAAATCCATGGACCGACCCCATTGTCTCCACCCCGTAGGAACTACGAGATCACCCC criterion=fanout-score sequence-density=0.01 sequence-density-rank=40 fanout-score=105.27 fanout-score-rank=1 prefix-density=0.28 prefix-fanout=2.5 sequence=GTTCCTTTTAGTTTCGACTTTAGGGATAATTTTTTTCGCTATCTTCTTCCGAGAACCGCCTAAGGTTCCAACTAAAAGAGTAAAATAATTTTATGGAAGTAAGAAGTCTACCCATCTGGTAGACTTCTTACTTCCATTAGTCCCCGTGTTCTTCGAATGGATCTCTTAATTGTTGAGAGGGTTGCCCAAACGCGGTATATAAGGCATACCCAGTAAAGCTTACAAGTAAACCAGATATGGAGATGGCGACTAAAGTTGCTGTTTCCATTTTTATAGAATTTAAAGATTACAATGGATCTACAAAAAGATCGT ERR11006575 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 17:51:05 Started mapping on | Dec 06 17:51:05 Finished on | Dec 06 17:56:23 Mapping speed, Million of reads per hour | 587.85 Number of input reads | 51927150 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 37382509 Uniquely mapped reads % | 71.99% Average mapped length | 298.49 Number of splices: Total | 12356991 Number of splices: Annotated (sjdb) | 11491692 Number of splices: GT/AG | 12016409 Number of splices: GC/AG | 151043 Number of splices: AT/AC | 17258 Number of splices: Non-canonical | 172281 Mismatch rate per base, % | 0.32% Deletion rate per base | 0.01% Deletion average length | 1.77 Insertion rate per base | 0.01% Insertion average length | 1.92 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 12977533 % of reads mapped to multiple loci | 24.99% Number of reads mapped to too many loci | 5219 % of reads mapped to too many loci | 0.01% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.87% % of reads unmapped: other | 0.14% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1567108 1567108 1567108 N_multimapping 12977533 12977533 12977533 N_noFeature 9534262 33924066 11835179 N_ambiguous 2069275 68401 938316 UnstrandedReadsAssigned:25778972 PositiveStrandReadsAssigned:3390042 NegativeStrandReadsAssigned:24609014 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006575 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006575-trimmed-pair1.fastq ERR11006575-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 51,927,150 reads, 30,928,442 reads pseudoaligned [quant] estimated average fragment length: 304.274 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,189 rounds 52973 ERR11006575.ke.tsv 35125 ERR11006575.se.tsv 88098 total ==> ERR11006575.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 633.085 0 0 PNS24247 1044 740.726 17.69 0.730146 PNS24249 1928 1624.73 101.606 1.91196 PNS24246 1044 740.726 17.69 0.730146 PNS24248 1044 740.726 17.69 0.730146 PNS24244 1471 1167.73 16.3242 0.427397 PNS24243 293 45.5239 0 0 KQK14069 1603 1299.73 3333.07 78.4031 KQK14071 474 174.72 34.0629 5.96043 ==> ERR11006575.se.tsv <== BRADI_1g14170v3 3611 BRADI_1g53295v3 53 BRADI_1g59795v3 175 BRADI_1g07683v3 0 BRADI_1g00485v3 1 BRADI_1g20270v3 66 BRADI_1g74790v3 146 BRADI_1g09890v3 0 BRADI_1g77505v3 185 BRADI_1g48960v3 0 ERR11006575 completed mapping pipeline successfully