Starting /dee2/code/volunteer_pipeline.sh ERR11006576 current disk space = 1550724759552 free memory = 1338785240 ERR11006576 SRAfilesize d2b8c7a27535d6c88c243050c989f5ac ERR11006576.sra ERR11006576.sra file validated ERR11006576 is paired end ERR11006576 is conventional basespace ERR11006576 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006576_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.3725 37.0 37.0 37.0 37.0 37.0 2 36.2805 37.0 37.0 37.0 37.0 37.0 3 36.3565 37.0 37.0 37.0 37.0 37.0 4 36.5415 37.0 37.0 37.0 37.0 37.0 5 36.448 37.0 37.0 37.0 37.0 37.0 6 36.554 37.0 37.0 37.0 37.0 37.0 7 36.4695 37.0 37.0 37.0 37.0 37.0 8 36.3575 37.0 37.0 37.0 37.0 37.0 9 36.459 37.0 37.0 37.0 37.0 37.0 10-14 36.4756 37.0 37.0 37.0 37.0 37.0 15-19 36.5188 37.0 37.0 37.0 37.0 37.0 20-24 36.4736 37.0 37.0 37.0 37.0 37.0 25-29 36.367200000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.3698 37.0 37.0 37.0 37.0 37.0 35-39 36.3184 37.0 37.0 37.0 37.0 37.0 40-44 36.3175 37.0 37.0 37.0 37.0 37.0 45-49 36.303000000000004 37.0 37.0 37.0 37.0 37.0 50-54 36.2494 37.0 37.0 37.0 37.0 37.0 55-59 36.2402 37.0 37.0 37.0 37.0 37.0 60-64 36.1184 37.0 37.0 37.0 37.0 37.0 65-69 36.1593 37.0 37.0 37.0 37.0 37.0 70-74 36.1304 37.0 37.0 37.0 37.0 37.0 75-79 36.1191 37.0 37.0 37.0 37.0 37.0 80-84 36.120400000000004 37.0 37.0 37.0 37.0 37.0 85-89 36.09160000000001 37.0 37.0 37.0 37.0 37.0 90-94 35.897299999999994 37.0 37.0 37.0 37.0 37.0 95-99 35.9679 37.0 37.0 37.0 37.0 37.0 100-104 35.9888 37.0 37.0 37.0 37.0 37.0 105-109 35.9161 37.0 37.0 37.0 37.0 37.0 110-114 35.863600000000005 37.0 37.0 37.0 37.0 37.0 115-119 35.9502 37.0 37.0 37.0 37.0 37.0 120-124 35.7328 37.0 37.0 37.0 37.0 37.0 125-129 35.715500000000006 37.0 37.0 37.0 37.0 37.0 130-134 35.7154 37.0 37.0 37.0 37.0 37.0 135-139 35.585699999999996 37.0 37.0 37.0 37.0 37.0 140-144 35.591499999999996 37.0 37.0 37.0 37.0 37.0 145-149 35.3198 37.0 37.0 37.0 34.6 37.0 150 35.5125 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.0 22 2.0 23 1.0 24 2.0 25 4.0 26 4.0 27 12.0 28 13.0 29 26.0 30 37.0 31 38.0 32 63.0 33 87.0 34 125.0 35 389.0 36 3036.0 37 159.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.15 11.200000000000001 13.875000000000002 35.775 2 27.87787787787788 10.46046046046046 26.151151151151154 35.510510510510514 3 22.375 11.975 29.375 36.275 4 26.674999999999997 14.325 26.3 32.7 5 27.35 18.25 27.950000000000003 26.450000000000003 6 25.575 26.075 21.95 26.400000000000002 7 16.825000000000003 27.125 35.5 20.549999999999997 8 16.975 26.700000000000003 36.4 19.925 9 16.650000000000002 27.450000000000003 36.55 19.35 10-14 19.835 30.875000000000004 27.755000000000003 21.535 15-19 19.555 33.775 25.474999999999998 21.195 20-24 18.945 29.215000000000003 28.595 23.244999999999997 25-29 21.349999999999998 31.535000000000004 26.305 20.810000000000002 30-34 23.735 29.785 25.679999999999996 20.8 35-39 22.725 31.369999999999997 26.13 19.775000000000002 40-44 19.52 30.45 25.88 24.15 45-49 20.05 28.685 28.07 23.195 50-54 20.02 31.840000000000003 26.445 21.695 55-59 21.58 29.57 23.765 25.085 60-64 19.875 30.915 25.480000000000004 23.73 65-69 20.49 30.175 25.230000000000004 24.104999999999997 70-74 22.165000000000003 30.159999999999997 22.82 24.855 75-79 22.645 28.82 25.330000000000002 23.205000000000002 80-84 22.305 28.51 25.314999999999998 23.87 85-89 24.855 28.24 24.884999999999998 22.02 90-94 21.310000000000002 29.29 27.295 22.105 95-99 23.86 29.49 23.400000000000002 23.25 100-104 21.41 30.459999999999997 24.505 23.625 105-109 21.535 29.065 25.77 23.630000000000003 110-114 24.025 27.665 24.855 23.455000000000002 115-119 20.23 29.84 26.16 23.77 120-124 21.12 28.48 24.83 25.569999999999997 125-129 20.765 30.904999999999998 22.805 25.525 130-134 23.419999999999998 30.185000000000002 24.82 21.575 135-139 23.305 29.354999999999997 23.11 24.23 140-144 24.32 28.355000000000004 26.125 21.2 145-149 22.97 30.035 24.875 22.12 150 21.55 28.725 27.675 22.05 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 0.5 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 1.0 19 1.5 20 1.0 21 1.5 22 3.5 23 5.5 24 8.0 25 9.0 26 8.5 27 7.0 28 6.5 29 12.0 30 19.0 31 24.5 32 29.5 33 35.5 34 41.5 35 40.0 36 70.0 37 147.0 38 277.0 39 290.5 40 261.5 41 309.0 42 278.0 43 236.0 44 211.0 45 233.0 46 215.0 47 137.0 48 117.0 49 95.0 50 71.5 51 63.5 52 56.0 53 48.0 54 34.0 55 33.5 56 34.5 57 25.5 58 24.5 59 24.5 60 26.0 61 28.0 62 26.5 63 24.5 64 42.0 65 59.0 66 41.0 67 28.5 68 21.5 69 16.0 70 17.5 71 19.0 72 21.0 73 17.0 74 11.0 75 9.5 76 8.5 77 7.5 78 6.5 79 6.0 80 5.0 81 2.5 82 2.0 83 1.0 84 1.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.1 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.949999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 79.82525814138205 50.24999999999999 2 13.343923749007146 16.8 3 2.5416997617156474 4.8 4 1.0325655281969817 2.6 5 0.7942811755361397 2.5 6 0.5162827640984908 1.95 7 0.23828435266084197 1.05 8 0.3177124702144559 1.6 9 0.11914217633042098 0.675 >10 1.2311358220810167 14.274999999999999 >50 0.0 0.0 >100 0.03971405877680699 3.5000000000000004 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 140 3.5000000000000004 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 48 1.2 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 43 1.075 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 33 0.8250000000000001 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 30 0.75 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 28 0.7000000000000001 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 28 0.7000000000000001 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 24 0.6 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 24 0.6 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 21 0.525 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 21 0.525 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 21 0.525 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 18 0.44999999999999996 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 18 0.44999999999999996 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 16 0.4 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 15 0.375 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 15 0.375 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 14 0.35000000000000003 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 14 0.35000000000000003 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 12 0.3 No Hit TCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGT 12 0.3 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 12 0.3 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 12 0.3 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 11 0.27499999999999997 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 11 0.27499999999999997 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 10 0.25 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 10 0.25 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 10 0.25 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 10 0.25 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 10 0.25 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 10 0.25 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 10 0.25 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 9 0.22499999999999998 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 9 0.22499999999999998 No Hit GGGGAATTCGTAGATCCTCCAGACGTAGAGCACGTAGGGCTTTGAAACCA 9 0.22499999999999998 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 8 0.2 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 8 0.2 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 8 0.2 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 8 0.2 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 8 0.2 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 8 0.2 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 8 0.2 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 8 0.2 No Hit ACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGA 7 0.17500000000000002 No Hit GGAGCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACT 7 0.17500000000000002 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 7 0.17500000000000002 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 7 0.17500000000000002 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 7 0.17500000000000002 No Hit TAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGA 7 0.17500000000000002 No Hit GCAGTCAATTAGAAGAATAAAGAAGAATTACTGCATTTCGTAACTTATTT 6 0.15 No Hit CCCTCTTCAAATAGATCTAATGGATAAGCTACATAACAGATCCATTGACT 6 0.15 No Hit ACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 6 0.15 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 6 0.15 No Hit TAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTA 6 0.15 No Hit TGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGA 6 0.15 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 6 0.15 No Hit TGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCT 6 0.15 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 6 0.15 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 6 0.15 No Hit CACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAAT 6 0.15 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 6 0.15 No Hit GCTCTCTTCATCATTTCTTCACATGTACCCGCAGTTGCATTCAAGTAATG 6 0.15 No Hit ACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTG 5 0.125 No Hit TAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCA 5 0.125 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 5 0.125 No Hit TGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATT 5 0.125 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 5 0.125 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 5 0.125 No Hit ACCCTCTTCAAATAGATCTAATGGATAAGCTACATAACAGATCCATTGAC 5 0.125 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 5 0.125 No Hit TCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGT 5 0.125 No Hit CCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAA 5 0.125 No Hit AGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCAT 5 0.125 No Hit CCCACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACA 5 0.125 No Hit TCCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 5 0.125 No Hit GGCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGAT 5 0.125 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 5 0.125 No Hit GTCATATATTCCGCTGAACGTCGTTCTTGCCAAGGTTGTATGTCTTTTTT 5 0.125 No Hit CCTCCACCAAATTGTAATACAGAATCATCCCCAAAGATTTCGGTCAGAGC 5 0.125 No Hit CACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCA 5 0.125 No Hit CCTAAAGTTAAGGATTTATCAATGGGTAATGTTGCTCCAATACCTAACCA 5 0.125 No Hit AACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.025 16-17 0.0 0.0 0.0 0.0 0.025 18-19 0.0 0.0 0.0 0.0 0.025 20-21 0.0 0.0 0.0 0.0 0.025 22-23 0.0 0.0 0.0 0.0 0.025 24-25 0.0 0.0 0.0 0.0 0.025 26-27 0.0 0.0 0.0 0.0 0.025 28-29 0.0 0.0 0.0 0.0 0.025 30-31 0.0 0.0 0.0 0.0 0.025 32-33 0.0 0.0 0.0 0.0 0.025 34-35 0.0 0.0 0.0 0.0 0.025 36-37 0.0 0.0 0.0 0.0 0.025 38-39 0.0 0.0 0.0 0.0 0.025 40-41 0.0 0.0 0.0 0.0 0.025 42-43 0.0 0.0 0.0 0.0 0.025 44-45 0.0 0.0 0.0 0.0 0.025 46-47 0.0 0.0 0.0 0.0 0.025 48-49 0.0 0.0 0.0 0.0 0.025 50-51 0.0 0.0 0.0 0.0 0.025 52-53 0.0 0.0 0.0 0.0 0.025 54-55 0.0 0.0 0.0 0.0 0.025 56-57 0.0 0.0 0.0 0.0 0.025 58-59 0.0 0.0 0.0 0.0 0.025 60-61 0.0 0.0 0.0 0.0 0.025 62-63 0.0 0.0 0.0 0.0 0.025 64-65 0.0 0.0 0.0 0.0 0.025 66-67 0.0 0.0 0.0 0.0 0.025 68-69 0.0 0.0 0.0 0.0 0.025 70-71 0.0 0.0 0.0 0.0 0.025 72-73 0.0 0.0 0.0 0.0 0.025 74-75 0.0 0.0 0.0 0.0 0.025 76-77 0.0 0.0 0.0 0.0 0.025 78-79 0.0 0.0 0.0 0.0 0.025 80-81 0.0 0.0 0.0 0.0 0.025 82-83 0.0 0.0 0.0 0.0 0.025 84-85 0.0 0.0 0.0 0.0 0.025 86-87 0.0 0.0 0.0 0.0 0.025 88-89 0.0 0.0 0.0 0.0 0.025 90-91 0.0 0.0 0.0 0.0 0.025 92-93 0.0 0.0 0.0 0.0 0.025 94-95 0.0 0.0 0.0 0.0 0.025 96-97 0.0 0.0 0.0 0.0 0.025 98-99 0.0 0.0 0.0 0.0 0.025 100-101 0.0 0.0 0.0 0.0 0.025 102-103 0.0 0.0 0.0 0.0 0.025 104-105 0.025 0.0 0.0 0.0 0.025 106-107 0.025 0.0 0.0 0.0 0.025 108-109 0.025 0.0 0.0 0.0 0.025 110-111 0.025 0.0 0.0 0.0 0.025 112-113 0.025 0.0 0.0 0.0 0.025 114-115 0.025 0.0 0.0 0.0 0.025 116-117 0.025 0.0 0.0 0.0 0.025 118-119 0.025 0.0 0.0 0.0 0.025 120-121 0.025 0.0 0.0 0.0 0.025 122-123 0.025 0.0 0.0 0.0 0.025 124-125 0.025 0.0 0.0 0.0 0.025 126-127 0.037500000000000006 0.0 0.0 0.0 0.025 128-129 0.05 0.0 0.0 0.0 0.025 130-131 0.05 0.0 0.0 0.0 0.025 132-133 0.05 0.0 0.0 0.0 0.025 134-135 0.05 0.0 0.0 0.0 0.025 136-137 0.05 0.0 0.0 0.0 0.025 138 0.05 0.0 0.0 0.0 0.025 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTTTCC 20 0.006139246 28.8 35-39 CCAGAAA 20 0.006139246 28.8 55-59 GTTTCCA 20 0.006139246 28.8 35-39 AATAGCA 20 0.006139246 28.8 15-19 AGTAAAG 20 0.006139246 28.8 45-49 CTTATCC 20 0.006139246 28.8 40-44 AGAGATA 20 0.006139246 28.8 25-29 AATATTG 20 0.006139246 28.8 30-34 GAATAAT 20 0.006139246 28.8 10-14 ATATTGT 20 0.006139246 28.8 30-34 GGAATAA 20 0.006139246 28.8 10-14 GAGATAA 20 0.006139246 28.8 25-29 AGATAAT 20 0.006139246 28.8 25-29 ACCAGAG 20 0.006139246 28.8 20-24 GCGATAA 35 0.0036813593 20.571428 110-114 ACCATCA 40 0.007966741 18.0 75-79 >>END_MODULE ERR11006576 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006576_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1745 37.0 37.0 37.0 37.0 37.0 2 36.1425 37.0 37.0 37.0 37.0 37.0 3 36.2225 37.0 37.0 37.0 37.0 37.0 4 36.186 37.0 37.0 37.0 37.0 37.0 5 36.1685 37.0 37.0 37.0 37.0 37.0 6 36.1155 37.0 37.0 37.0 37.0 37.0 7 36.1535 37.0 37.0 37.0 37.0 37.0 8 36.2425 37.0 37.0 37.0 37.0 37.0 9 36.253 37.0 37.0 37.0 37.0 37.0 10-14 36.2495 37.0 37.0 37.0 37.0 37.0 15-19 36.25840000000001 37.0 37.0 37.0 37.0 37.0 20-24 36.1502 37.0 37.0 37.0 37.0 37.0 25-29 36.1294 37.0 37.0 37.0 37.0 37.0 30-34 36.13170000000001 37.0 37.0 37.0 37.0 37.0 35-39 36.0616 37.0 37.0 37.0 37.0 37.0 40-44 36.0441 37.0 37.0 37.0 37.0 37.0 45-49 35.991 37.0 37.0 37.0 37.0 37.0 50-54 36.0353 37.0 37.0 37.0 37.0 37.0 55-59 35.929500000000004 37.0 37.0 37.0 37.0 37.0 60-64 35.8596 37.0 37.0 37.0 37.0 37.0 65-69 35.780199999999994 37.0 37.0 37.0 37.0 37.0 70-74 35.8528 37.0 37.0 37.0 37.0 37.0 75-79 35.833600000000004 37.0 37.0 37.0 37.0 37.0 80-84 35.7313 37.0 37.0 37.0 37.0 37.0 85-89 35.7128 37.0 37.0 37.0 37.0 37.0 90-94 35.530100000000004 37.0 37.0 37.0 37.0 37.0 95-99 35.4439 37.0 37.0 37.0 37.0 37.0 100-104 35.5143 37.0 37.0 37.0 37.0 37.0 105-109 35.3215 37.0 37.0 37.0 32.2 37.0 110-114 35.404900000000005 37.0 37.0 37.0 32.2 37.0 115-119 35.2175 37.0 37.0 37.0 27.4 37.0 120-124 35.3823 37.0 37.0 37.0 34.6 37.0 125-129 35.190599999999996 37.0 37.0 37.0 29.8 37.0 130-134 35.1508 37.0 37.0 37.0 25.0 37.0 135-139 35.126799999999996 37.0 37.0 37.0 25.0 37.0 140-144 34.9457 37.0 37.0 37.0 25.0 37.0 145-149 34.9243 37.0 37.0 37.0 25.0 37.0 150 34.569 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 1.0 20 1.0 21 1.0 22 2.0 23 4.0 24 4.0 25 8.0 26 14.0 27 15.0 28 20.0 29 25.0 30 37.0 31 48.0 32 41.0 33 115.0 34 255.0 35 920.0 36 2420.0 37 68.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.925 26.150000000000002 17.5 28.425 2 26.200000000000003 25.374999999999996 32.25 16.175 3 19.900000000000002 27.500000000000004 33.575 19.025 4 23.400000000000002 28.025 27.950000000000003 20.625 5 23.375 29.099999999999998 28.125 19.400000000000002 6 21.725 33.45 27.55 17.275 7 20.025000000000002 21.25 39.574999999999996 19.15 8 21.275 23.599999999999998 30.8 24.325 9 23.0 21.45 34.949999999999996 20.599999999999998 10-14 23.580000000000002 26.150000000000002 28.975 21.295 15-19 23.625 25.605 30.025000000000002 20.745 20-24 24.099999999999998 24.485 29.615000000000002 21.8 25-29 23.990000000000002 24.545 30.39 21.075 30-34 23.89 23.695 30.995 21.42 35-39 23.585 24.425 30.580000000000002 21.41 40-44 22.8 25.245 30.36 21.595 45-49 22.900000000000002 26.640000000000004 28.884999999999998 21.575 50-54 22.68 26.11 28.83 22.38 55-59 22.985 24.995 28.715000000000003 23.305 60-64 23.544999999999998 24.79 29.465000000000003 22.2 65-69 24.145 24.89 28.970000000000002 21.995 70-74 24.205 25.14 29.625 21.029999999999998 75-79 23.74 24.310000000000002 30.080000000000002 21.87 80-84 24.67 25.145 29.07 21.115000000000002 85-89 25.174999999999997 24.805 27.779999999999998 22.24 90-94 23.805 24.5 29.360000000000003 22.335 95-99 24.349999999999998 25.174999999999997 29.01 21.465 100-104 24.654999999999998 24.445 29.425 21.475 105-109 25.16 23.86 28.975 22.005 110-114 23.52 24.745 29.59 22.145 115-119 23.94 25.4 28.439999999999998 22.220000000000002 120-124 24.095 25.395 28.82 21.69 125-129 23.395 25.555 29.555 21.495 130-134 23.75 25.75 28.515 21.985 135-139 24.175 24.91 29.53 21.385 140-144 23.375 24.58 30.575000000000003 21.47 145-149 23.66 23.985 30.525000000000002 21.83 150 24.224999999999998 23.849999999999998 28.925 23.0 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 1.5 21 2.5 22 1.5 23 2.0 24 6.0 25 10.0 26 11.0 27 10.5 28 13.0 29 15.5 30 21.0 31 36.0 32 43.5 33 44.0 34 58.0 35 76.0 36 115.0 37 191.0 38 231.5 39 205.0 40 229.0 41 263.5 42 243.0 43 243.5 44 233.5 45 207.5 46 181.0 47 145.0 48 115.0 49 87.5 50 73.5 51 63.0 52 43.5 53 30.0 54 34.5 55 38.5 56 36.5 57 35.0 58 28.5 59 31.5 60 32.0 61 29.5 62 30.5 63 38.0 64 51.0 65 44.0 66 32.0 67 32.5 68 32.0 69 31.0 70 32.5 71 24.0 72 20.0 73 23.0 74 18.0 75 19.0 76 14.5 77 7.5 78 8.0 79 7.5 80 5.0 81 3.0 82 2.5 83 1.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.39999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 80.36743515850145 55.775 2 11.995677233429394 16.650000000000002 3 3.314121037463977 6.9 4 1.2247838616714697 3.4000000000000004 5 0.9365994236311239 3.25 6 0.6844380403458213 2.85 7 0.43227665706051877 2.1 8 0.18011527377521613 1.0 9 0.3242074927953891 2.025 >10 0.5403458213256485 6.05 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 48 1.2 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 32 0.8 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 18 0.44999999999999996 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 15 0.375 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 14 0.35000000000000003 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 13 0.325 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 13 0.325 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 12 0.3 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 12 0.3 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 12 0.3 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 11 0.27499999999999997 No Hit AGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCT 11 0.27499999999999997 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 11 0.27499999999999997 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 10 0.25 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 10 0.25 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 9 0.22499999999999998 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 9 0.22499999999999998 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 9 0.22499999999999998 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 9 0.22499999999999998 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 9 0.22499999999999998 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 9 0.22499999999999998 No Hit AATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTA 9 0.22499999999999998 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 9 0.22499999999999998 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 9 0.22499999999999998 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 8 0.2 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 8 0.2 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 8 0.2 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 8 0.2 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 8 0.2 No Hit CCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGCTGC 7 0.17500000000000002 No Hit TGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGA 7 0.17500000000000002 No Hit CGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGT 7 0.17500000000000002 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 7 0.17500000000000002 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 7 0.17500000000000002 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 7 0.17500000000000002 No Hit GATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTC 7 0.17500000000000002 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 7 0.17500000000000002 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 7 0.17500000000000002 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 7 0.17500000000000002 No Hit GCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGC 7 0.17500000000000002 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 7 0.17500000000000002 No Hit TGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCT 6 0.15 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 6 0.15 No Hit GCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTTATG 6 0.15 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 6 0.15 No Hit CCAGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTA 6 0.15 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 6 0.15 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 6 0.15 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 6 0.15 No Hit AGCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTG 6 0.15 No Hit CAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCT 6 0.15 No Hit TATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACC 6 0.15 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 6 0.15 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 6 0.15 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 6 0.15 No Hit CTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTAC 6 0.15 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 6 0.15 No Hit TGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACT 6 0.15 No Hit TCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTG 6 0.15 No Hit AGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGC 6 0.15 No Hit CGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTT 5 0.125 No Hit ATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGC 5 0.125 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 5 0.125 No Hit GCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCA 5 0.125 No Hit CAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGT 5 0.125 No Hit AATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACT 5 0.125 No Hit TGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATA 5 0.125 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 5 0.125 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 5 0.125 No Hit GACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCC 5 0.125 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 5 0.125 No Hit CTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAG 5 0.125 No Hit ATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATATT 5 0.125 No Hit TCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGGCGGT 5 0.125 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 5 0.125 No Hit TATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGT 5 0.125 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 5 0.125 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 5 0.125 No Hit TGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATT 5 0.125 No Hit TGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATT 5 0.125 No Hit CATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGGCG 5 0.125 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 5 0.125 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 5 0.125 No Hit GAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTT 5 0.125 No Hit AGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATG 5 0.125 No Hit GTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.025 0.0 0.0 0.0 0.0 106-107 0.025 0.0 0.0 0.0 0.0 108-109 0.025 0.0 0.0 0.0 0.0 110-111 0.025 0.0 0.0 0.0 0.0 112-113 0.025 0.0 0.0 0.0 0.0 114-115 0.025 0.0 0.0 0.0 0.0 116-117 0.025 0.0 0.0 0.0 0.0 118-119 0.025 0.0 0.0 0.0 0.0 120-121 0.025 0.0 0.0 0.0 0.0 122-123 0.025 0.0 0.0 0.0 0.0 124-125 0.025 0.0 0.0 0.0 0.0 126-127 0.037500000000000006 0.0 0.0 0.0 0.0 128-129 0.05 0.0 0.0 0.0 0.0 130-131 0.05 0.0 0.0 0.0 0.0 132-133 0.05 0.0 0.0 0.0 0.0 134-135 0.05 0.0 0.0 0.0 0.0 136-137 0.075 0.0 0.0 0.0 0.0 138 0.075 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCATGTA 10 0.006973645 144.0 7 >>END_MODULE Read 2977474 spots for ERR11006576.sra Written 2977474 spots for ERR11006576.sra Read 2977474 spots for ERR11006576.sra Written 2977474 spots for ERR11006576.sra Read 2977474 spots for ERR11006576.sra Written 2977474 spots for ERR11006576.sra Read 2977474 spots for ERR11006576.sra Written 2977474 spots for ERR11006576.sra Read 2977474 spots for ERR11006576.sra Written 2977474 spots for ERR11006576.sra Read 2977474 spots for ERR11006576.sra Written 2977474 spots for ERR11006576.sra Read 2977474 spots for ERR11006576.sra Written 2977474 spots for ERR11006576.sra Read 2977474 spots for ERR11006576.sra Written 2977474 spots for ERR11006576.sra Read 2977474 spots for ERR11006576.sra Written 2977474 spots for ERR11006576.sra Read 2977493 spots for ERR11006576.sra Written 2977493 spots for ERR11006576.sra Read 2977474 spots for ERR11006576.sra Written 2977474 spots for ERR11006576.sra Read 2977474 spots for ERR11006576.sra Written 2977474 spots for ERR11006576.sra Read 2977474 spots for ERR11006576.sra Written 2977474 spots for ERR11006576.sra Read 2977474 spots for ERR11006576.sra Written 2977474 spots for ERR11006576.sra Read 2977474 spots for ERR11006576.sra Written 2977474 spots for ERR11006576.sra Read 2977474 spots for ERR11006576.sra Written 2977474 spots for ERR11006576.sra Read 2977474 spots for ERR11006576.sra Written 2977474 spots for ERR11006576.sra Read 2977474 spots for ERR11006576.sra Written 2977474 spots for ERR11006576.sra Read 2977474 spots for ERR11006576.sra Written 2977474 spots for ERR11006576.sra Read 2977474 spots for ERR11006576.sra Written 2977474 spots for ERR11006576.sra SRR ids: ['ERR11006576.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_7j_wz691 ERR11006576.sra spots: 59549499 blocks: [[1, 2977474], [2977475, 5954948], [5954949, 8932422], [8932423, 11909896], [11909897, 14887370], [14887371, 17864844], [17864845, 20842318], [20842319, 23819792], [23819793, 26797266], [26797267, 29774740], [29774741, 32752214], [32752215, 35729688], [35729689, 38707162], [38707163, 41684636], [41684637, 44662110], [44662111, 47639584], [47639585, 50617058], [50617059, 53594532], [53594533, 56572006], [56572007, 59549499]] ERR11006576 file size 21882883 ERR11006576 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006576 ERR11006576_1.fastq ERR11006576_2.fastq Input file: ERR11006576_1.fastq Paired file: ERR11006576_2.fastq trimmed: ERR11006576-trimmed-pair1.fastq, ERR11006576-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 17:51:23 2024 >> started Fri Dec 6 17:52:55 2024 >> done (91.591s) 59549499 read pairs processed; of these: 248 ( 0.00%) short read pairs filtered out after trimming by size control 614 ( 0.00%) empty read pairs filtered out after trimming by size control 59548637 (100.00%) read pairs available; of these: 174790 ( 0.29%) trimmed read pairs available after processing 59373847 (99.71%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 16 0.00% 19 12 0.00% 20 16 0.00% 21 20 0.00% 22 46 0.00% 23 17 0.00% 24 22 0.00% 25 13 0.00% 26 16 0.00% 27 32 0.00% 28 26 0.00% 29 21 0.00% 30 36 0.00% 31 50 0.00% 32 24 0.00% 33 36 0.00% 34 40 0.00% 35 42 0.00% 36 29 0.00% 37 29 0.00% 38 37 0.00% 39 32 0.00% 40 43 0.00% 41 29 0.00% 42 37 0.00% 43 38 0.00% 44 63 0.00% 45 48 0.00% 46 50 0.00% 47 43 0.00% 48 49 0.00% 49 49 0.00% 50 74 0.00% 51 50 0.00% 52 61 0.00% 53 67 0.00% 54 62 0.00% 55 77 0.00% 56 66 0.00% 57 78 0.00% 58 78 0.00% 59 89 0.00% 60 89 0.00% 61 82 0.00% 62 96 0.00% 63 107 0.00% 64 106 0.00% 65 116 0.00% 66 133 0.00% 67 129 0.00% 68 146 0.00% 69 139 0.00% 70 157 0.00% 71 163 0.00% 72 156 0.00% 73 151 0.00% 74 182 0.00% 75 186 0.00% 76 196 0.00% 77 207 0.00% 78 217 0.00% 79 235 0.00% 80 252 0.00% 81 238 0.00% 82 276 0.00% 83 321 0.00% 84 319 0.00% 85 346 0.00% 86 364 0.00% 87 386 0.00% 88 415 0.00% 89 455 0.00% 90 489 0.00% 91 465 0.00% 92 549 0.00% 93 522 0.00% 94 562 0.00% 95 606 0.00% 96 627 0.00% 97 654 0.00% 98 751 0.00% 99 722 0.00% 100 864 0.00% 101 886 0.00% 102 820 0.00% 103 955 0.00% 104 957 0.00% 105 1051 0.00% 106 1095 0.00% 107 1207 0.00% 108 1180 0.00% 109 1304 0.00% 110 1432 0.00% 111 1391 0.00% 112 1663 0.00% 113 1563 0.00% 114 1608 0.00% 115 1675 0.00% 116 1903 0.00% 117 2002 0.00% 118 1947 0.00% 119 2090 0.00% 120 2401 0.00% 121 2414 0.00% 122 2645 0.00% 123 2692 0.00% 124 2718 0.00% 125 3219 0.01% 126 3074 0.01% 127 2979 0.01% 128 2965 0.00% 129 3442 0.01% 130 3682 0.01% 131 3714 0.01% 132 3980 0.01% 133 3825 0.01% 134 4069 0.01% 135 3717 0.01% 136 4049 0.01% 137 4161 0.01% 138 4395 0.01% 139 4772 0.01% 140 4864 0.01% 141 5144 0.01% 142 5704 0.01% 143 5939 0.01% 144 6234 0.01% 145 6277 0.01% 146 7621 0.01% 147 7457 0.01% 148 7244 0.01% 149 7725 0.01% 150 59373847 99.71% 59548637 reads passed initial QC criterion=sequence-density sequence-density=0.28 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=33 prefix-density=0.26 prefix-fanout=2.0 sequence=TCTAATTCAAAA criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=70.17 fanout-score-rank=1 prefix-density=0.30 prefix-fanout=2.0 sequence=AAAAACAGTAGAAGTAGAACAGGTATAAATAAGAAAATCTTAGTTAAGAGGGTTCATGTAAAGAACAGGTTCTAAATCACGATCGATTCCCTTTTCAAAACCTGCTGCAGCAGCTCGGGCTCTTCCTGCATGCCACAAATGGCCCACAAAAAAGAAGAATCCTAGAACAAAATGAGAAGTCGATAACCAACTTCTAGGAGAGACATAATTAACTGCATTGATCTCGGTAGCTACGCCACCCACGGAATTTAAAGAGCCTAAAGGAGCATGGGTCATATATTCCGCTGAACGTCGTTCTTGCCAAGGTTGTATGTCTTTTTTCAACCTACTCAAGTCCAAACCGTTGGGCCCCCTTAGAGGTTCTAACCATGGAGCACGGAGGTCCCAAAAACGCATAGTTTCCCCTCCAAAGATAACCTCTCCCGTTGGGGAACGCATTAGATATTTACCTAAACCTGTGGGTCCTTGAGCAGATCCCACATTAGCTCCAAGACGCTGGTCTCTAACTAGA criterion=sequence-density sequence-density=0.32 sequence-density-rank=1 fanout-score=2.20 fanout-score-rank=29 prefix-density=0.32 prefix-fanout=2.2 sequence=GAACTACGAGATCACCCC criterion=fanout-score sequence-density=0.01 sequence-density-rank=42 fanout-score=121.84 fanout-score-rank=1 prefix-density=0.26 prefix-fanout=2.7 sequence=GTTCCTTTTAGTTTCGACTTTAGGGATAATTTTTTTCGCTATCTTCTTCCGAGAACCGCCTAAGGTTCCAACTAAAAGAGTAAAATAATTTTATGGAAGTAAGAAGTCTACCCATCTGGTAGACTTCTTACTTCCATTAGTCCCCGTGTTCTTCGAATGGATCTCTTAATTGTTGAGAGGGTTGCCCAAACGCGGTATATAAGGCATACCCAGTAAAGCTTACAAGTAAACCAGATATGGAGATGGCGACTAAAGTTGCTGTTTCCATTTTTATAGAATTTAAAGATTACAATGGATCTACAAAAAGATCGT ERR11006576 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 17:54:43 Started mapping on | Dec 06 17:54:43 Finished on | Dec 06 18:00:36 Mapping speed, Million of reads per hour | 607.29 Number of input reads | 59548637 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 44334878 Uniquely mapped reads % | 74.45% Average mapped length | 298.75 Number of splices: Total | 15051547 Number of splices: Annotated (sjdb) | 14047377 Number of splices: GT/AG | 14661802 Number of splices: GC/AG | 188917 Number of splices: AT/AC | 18869 Number of splices: Non-canonical | 181959 Mismatch rate per base, % | 0.29% Deletion rate per base | 0.01% Deletion average length | 1.78 Insertion rate per base | 0.01% Insertion average length | 1.94 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 13599412 % of reads mapped to multiple loci | 22.84% Number of reads mapped to too many loci | 7068 % of reads mapped to too many loci | 0.01% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.56% % of reads unmapped: other | 0.14% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1614347 1614347 1614347 N_multimapping 13599412 13599412 13599412 N_noFeature 11832975 39955366 14930668 N_ambiguous 2263612 81880 1007819 UnstrandedReadsAssigned:30238291 PositiveStrandReadsAssigned:4297632 NegativeStrandReadsAssigned:28396391 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006576 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006576-trimmed-pair1.fastq ERR11006576-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 59,548,637 reads, 34,203,482 reads pseudoaligned [quant] estimated average fragment length: 311.981 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,104 rounds 52973 ERR11006576.ke.tsv 35125 ERR11006576.se.tsv 88098 total ==> ERR11006576.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 625.265 0 0 PNS24247 1044 733.019 20.552 0.749806 PNS24249 1928 1617.02 131.365 2.17257 PNS24246 1044 733.019 20.552 0.749806 PNS24248 1044 733.019 20.552 0.749806 PNS24244 1471 1160.02 14.9793 0.345331 PNS24243 293 42.0654 2 1.2715 KQK14069 1603 1292.02 3893.5 80.5902 KQK14071 474 168.003 45.5016 7.24303 ==> ERR11006576.se.tsv <== BRADI_1g14170v3 4249 BRADI_1g53295v3 60 BRADI_1g59795v3 169 BRADI_1g07683v3 0 BRADI_1g00485v3 1 BRADI_1g20270v3 158 BRADI_1g74790v3 259 BRADI_1g09890v3 0 BRADI_1g77505v3 191 BRADI_1g48960v3 0 ERR11006576 completed mapping pipeline successfully