Starting /dee2/code/volunteer_pipeline.sh ERR11006577 current disk space = 1550711394304 free memory = 1596434756 ERR11006577 SRAfilesize 9a6bb8faf6ce059958fa72a65c8d8c8b ERR11006577.sra ERR11006577.sra file validated ERR11006577 is paired end ERR11006577 is conventional basespace ERR11006577 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006577_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.4645 37.0 37.0 37.0 37.0 37.0 2 36.33275 37.0 37.0 37.0 37.0 37.0 3 36.472 37.0 37.0 37.0 37.0 37.0 4 36.488 37.0 37.0 37.0 37.0 37.0 5 36.4845 37.0 37.0 37.0 37.0 37.0 6 36.5455 37.0 37.0 37.0 37.0 37.0 7 36.4 37.0 37.0 37.0 37.0 37.0 8 36.463 37.0 37.0 37.0 37.0 37.0 9 36.54 37.0 37.0 37.0 37.0 37.0 10-14 36.5019 37.0 37.0 37.0 37.0 37.0 15-19 36.5082 37.0 37.0 37.0 37.0 37.0 20-24 36.527699999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.4137 37.0 37.0 37.0 37.0 37.0 30-34 36.3845 37.0 37.0 37.0 37.0 37.0 35-39 36.3361 37.0 37.0 37.0 37.0 37.0 40-44 36.3374 37.0 37.0 37.0 37.0 37.0 45-49 36.34009999999999 37.0 37.0 37.0 37.0 37.0 50-54 36.2705 37.0 37.0 37.0 37.0 37.0 55-59 36.2382 37.0 37.0 37.0 37.0 37.0 60-64 36.1541 37.0 37.0 37.0 37.0 37.0 65-69 36.2 37.0 37.0 37.0 37.0 37.0 70-74 36.162499999999994 37.0 37.0 37.0 37.0 37.0 75-79 36.1811 37.0 37.0 37.0 37.0 37.0 80-84 36.17359999999999 37.0 37.0 37.0 37.0 37.0 85-89 36.1223 37.0 37.0 37.0 37.0 37.0 90-94 35.9347 37.0 37.0 37.0 37.0 37.0 95-99 35.9989 37.0 37.0 37.0 37.0 37.0 100-104 35.9968 37.0 37.0 37.0 37.0 37.0 105-109 35.933800000000005 37.0 37.0 37.0 37.0 37.0 110-114 35.8636 37.0 37.0 37.0 37.0 37.0 115-119 35.9478 37.0 37.0 37.0 37.0 37.0 120-124 35.7555 37.0 37.0 37.0 37.0 37.0 125-129 35.821200000000005 37.0 37.0 37.0 37.0 37.0 130-134 35.6826 37.0 37.0 37.0 37.0 37.0 135-139 35.5595 37.0 37.0 37.0 37.0 37.0 140-144 35.5677 37.0 37.0 37.0 37.0 37.0 145-149 35.371399999999994 37.0 37.0 37.0 34.6 37.0 150 35.5895 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 1.0 23 0.0 24 0.0 25 6.0 26 7.0 27 10.0 28 10.0 29 24.0 30 31.0 31 31.0 32 71.0 33 89.0 34 147.0 35 355.0 36 3070.0 37 147.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.4 11.600000000000001 13.275 35.725 2 28.907226806701676 9.552388097024256 26.331582895723933 35.20880220055014 3 22.15 11.774999999999999 28.725 37.35 4 28.499999999999996 14.7 25.6 31.2 5 29.25 16.975 27.250000000000004 26.525 6 22.975 29.25 22.425 25.35 7 17.2 29.349999999999998 33.525 19.925 8 16.35 27.500000000000004 35.425000000000004 20.724999999999998 9 18.625 27.325 36.3 17.75 10-14 20.405 31.630000000000003 26.724999999999998 21.240000000000002 15-19 20.005 35.075 23.905 21.015 20-24 18.66 29.599999999999998 28.675 23.064999999999998 25-29 21.745 32.65 25.275 20.330000000000002 30-34 24.125 31.165 25.1 19.61 35-39 22.785 31.805 24.965 20.445 40-44 19.66 31.259999999999998 25.05 24.03 45-49 19.11 29.409999999999997 28.095 23.385 50-54 20.65 33.165 25.324999999999996 20.86 55-59 22.275 30.470000000000002 22.715 24.54 60-64 19.345000000000002 32.245000000000005 24.605 23.805 65-69 20.555 30.320000000000004 24.955 24.169999999999998 70-74 23.205000000000002 30.145 21.175 25.474999999999998 75-79 22.715 29.53 24.81 22.945 80-84 22.61 29.025000000000002 24.58 23.785 85-89 25.259999999999998 28.95 24.455 21.335 90-94 21.634999999999998 29.354999999999997 27.29 21.72 95-99 24.990000000000002 29.82 22.23 22.96 100-104 21.52 30.764999999999997 24.779999999999998 22.935 105-109 21.63 28.475 26.825 23.07 110-114 24.725 27.76 24.490000000000002 23.025000000000002 115-119 19.8 30.84 25.905 23.455000000000002 120-124 20.085 30.214999999999996 24.884999999999998 24.815 125-129 20.185 32.79 21.745 25.28 130-134 23.185 30.135 25.569999999999997 21.11 135-139 24.21 29.65 22.225 23.915 140-144 25.31 28.425 26.334999999999997 19.93 145-149 23.49 30.409999999999997 25.0 21.099999999999998 150 24.175 25.074999999999996 29.2 21.55 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 1.0 21 1.0 22 1.0 23 0.5 24 1.0 25 2.5 26 4.0 27 6.5 28 10.5 29 11.0 30 9.5 31 14.5 32 18.5 33 20.0 34 31.0 35 48.5 36 74.5 37 147.5 38 300.0 39 313.5 40 286.0 41 337.0 42 292.0 43 240.0 44 204.5 45 207.5 46 210.0 47 165.0 48 115.0 49 76.0 50 65.5 51 59.5 52 47.0 53 35.0 54 35.0 55 32.5 56 26.5 57 27.5 58 29.0 59 24.0 60 26.0 61 30.5 62 24.5 63 22.5 64 53.5 65 82.5 66 52.0 67 25.5 68 24.0 69 15.0 70 16.0 71 22.0 72 16.0 73 9.5 74 8.0 75 10.0 76 8.5 77 5.5 78 5.0 79 3.0 80 2.0 81 1.0 82 1.0 83 1.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.525000000000006 #Duplication Level Percentage of deduplicated Percentage of total 1 80.05402971634399 44.45 2 12.246735704637551 13.600000000000001 3 2.746510580819451 4.575 4 0.8554705087798289 1.9 5 0.585321927059883 1.625 6 0.6303466906798739 2.1 7 0.36019810895992793 1.4000000000000001 8 0.27014858171994593 1.2 9 0.27014858171994593 1.35 >10 1.8009905447996397 19.35 >50 0.13507429085997297 4.5 >100 0.04502476361999099 3.95 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 158 3.95 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 68 1.7000000000000002 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 60 1.5 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 52 1.3 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 44 1.0999999999999999 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 44 1.0999999999999999 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 44 1.0999999999999999 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 41 1.0250000000000001 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 35 0.8750000000000001 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 34 0.8500000000000001 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 28 0.7000000000000001 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 28 0.7000000000000001 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 26 0.65 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 24 0.6 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 21 0.525 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 21 0.525 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 20 0.5 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 18 0.44999999999999996 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 18 0.44999999999999996 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 17 0.42500000000000004 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 16 0.4 No Hit GCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCC 16 0.4 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 16 0.4 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 16 0.4 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 16 0.4 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 15 0.375 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 15 0.375 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 15 0.375 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 15 0.375 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 14 0.35000000000000003 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 13 0.325 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 13 0.325 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 13 0.325 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 12 0.3 No Hit ATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATA 12 0.3 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 11 0.27499999999999997 No Hit ACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 11 0.27499999999999997 No Hit CCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAA 11 0.27499999999999997 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 11 0.27499999999999997 No Hit GGGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACA 10 0.25 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 10 0.25 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 10 0.25 No Hit TCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTT 10 0.25 No Hit TAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGA 10 0.25 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 9 0.22499999999999998 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 9 0.22499999999999998 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 9 0.22499999999999998 No Hit CACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCG 9 0.22499999999999998 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 9 0.22499999999999998 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 9 0.22499999999999998 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 8 0.2 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 8 0.2 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 8 0.2 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 8 0.2 No Hit TGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCT 8 0.2 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 8 0.2 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 7 0.17500000000000002 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 7 0.17500000000000002 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 7 0.17500000000000002 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 7 0.17500000000000002 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 7 0.17500000000000002 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 7 0.17500000000000002 No Hit CACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAAT 7 0.17500000000000002 No Hit GGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGG 7 0.17500000000000002 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 6 0.15 No Hit CACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGC 6 0.15 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 6 0.15 No Hit TGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGA 6 0.15 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 6 0.15 No Hit GGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAG 6 0.15 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 6 0.15 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 6 0.15 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 6 0.15 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 6 0.15 No Hit AACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAG 6 0.15 No Hit GTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAA 6 0.15 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 6 0.15 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 6 0.15 No Hit CTCCTATTCAATTACTTCAACCATTTCCGAGCACCTCGTATCACTTCCAA 5 0.125 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 5 0.125 No Hit CGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCT 5 0.125 No Hit ATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCT 5 0.125 No Hit TAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTA 5 0.125 No Hit CTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAG 5 0.125 No Hit GGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCT 5 0.125 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 5 0.125 No Hit CAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGA 5 0.125 No Hit TTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATATCT 5 0.125 No Hit GGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 5 0.125 No Hit CGCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATG 5 0.125 No Hit AGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.025 0.0 0.0 0.0 0.0 92-93 0.025 0.0 0.0 0.0 0.0 94-95 0.025 0.0 0.0 0.0 0.0 96-97 0.05 0.0 0.0 0.0 0.0 98-99 0.075 0.0 0.0 0.0 0.0 100-101 0.075 0.0 0.0 0.0 0.0 102-103 0.075 0.0 0.0 0.0 0.0 104-105 0.075 0.0 0.0 0.0 0.0 106-107 0.075 0.0 0.0 0.0 0.0 108-109 0.075 0.0 0.0 0.0 0.0 110-111 0.075 0.0 0.0 0.0 0.0 112-113 0.075 0.0 0.0 0.0 0.0 114-115 0.1 0.0 0.0 0.0 0.0 116-117 0.1 0.0 0.0 0.0 0.0 118-119 0.125 0.0 0.0 0.0 0.0 120-121 0.125 0.0 0.0 0.0 0.0 122-123 0.125 0.0 0.0 0.0 0.0 124-125 0.125 0.0 0.0 0.0 0.0 126-127 0.125 0.0 0.0 0.0 0.0 128-129 0.125 0.0 0.0 0.0 0.0 130-131 0.175 0.0 0.0 0.0 0.0 132-133 0.175 0.0 0.0 0.0 0.0 134-135 0.175 0.0 0.0 0.0 0.0 136-137 0.175 0.0 0.0 0.0 0.0 138 0.175 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGGTAG 10 0.006973645 144.0 3 ATCATAT 10 0.006973645 144.0 6 GCTTTCT 30 1.46121765E-5 96.0 1 TTCTTCA 35 3.1411873E-5 82.28571 9 CTTTTCT 40 6.0911432E-5 72.0 6 TTTCTTC 40 6.0911432E-5 72.0 8 TTTTCTT 40 6.0911432E-5 72.0 7 TTCTTTT 40 6.0911432E-5 72.0 4 TCTTTTC 40 6.0911432E-5 72.0 5 CTTTCTT 45 1.0917089E-4 64.0 2 TTTCTTT 50 1.838823E-4 57.6 3 AATTCTT 35 0.0036813593 20.571428 15-19 TATGTTA 35 0.0036813593 20.571428 25-29 TTATATG 35 0.0036813593 20.571428 20-24 TGTTAGC 35 0.0036813593 20.571428 25-29 TTCTTAT 35 0.0036813593 20.571428 20-24 CTTATAT 35 0.0036813593 20.571428 20-24 ATATGTT 35 0.0036813593 20.571428 25-29 ATGTTAG 35 0.0036813593 20.571428 25-29 TCTTCAA 35 0.0036813593 20.571428 6 >>END_MODULE ERR11006577 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006577_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.006 37.0 37.0 37.0 37.0 37.0 2 35.801 37.0 37.0 37.0 37.0 37.0 3 35.9175 37.0 37.0 37.0 37.0 37.0 4 35.846 37.0 37.0 37.0 37.0 37.0 5 35.9495 37.0 37.0 37.0 37.0 37.0 6 35.9855 37.0 37.0 37.0 37.0 37.0 7 35.861 37.0 37.0 37.0 37.0 37.0 8 35.9655 37.0 37.0 37.0 37.0 37.0 9 36.0575 37.0 37.0 37.0 37.0 37.0 10-14 36.0843 37.0 37.0 37.0 37.0 37.0 15-19 36.0258 37.0 37.0 37.0 37.0 37.0 20-24 36.0235 37.0 37.0 37.0 37.0 37.0 25-29 35.9621 37.0 37.0 37.0 37.0 37.0 30-34 35.9218 37.0 37.0 37.0 37.0 37.0 35-39 35.885799999999996 37.0 37.0 37.0 37.0 37.0 40-44 35.849900000000005 37.0 37.0 37.0 37.0 37.0 45-49 35.8316 37.0 37.0 37.0 37.0 37.0 50-54 35.773900000000005 37.0 37.0 37.0 37.0 37.0 55-59 35.723499999999994 37.0 37.0 37.0 37.0 37.0 60-64 35.642100000000006 37.0 37.0 37.0 37.0 37.0 65-69 35.556799999999996 37.0 37.0 37.0 37.0 37.0 70-74 35.5302 37.0 37.0 37.0 37.0 37.0 75-79 35.5577 37.0 37.0 37.0 37.0 37.0 80-84 35.5132 37.0 37.0 37.0 37.0 37.0 85-89 35.4036 37.0 37.0 37.0 34.6 37.0 90-94 35.2921 37.0 37.0 37.0 32.2 37.0 95-99 35.214299999999994 37.0 37.0 37.0 27.4 37.0 100-104 35.1637 37.0 37.0 37.0 27.4 37.0 105-109 35.0153 37.0 37.0 37.0 25.0 37.0 110-114 35.1169 37.0 37.0 37.0 25.0 37.0 115-119 34.7968 37.0 37.0 37.0 25.0 37.0 120-124 35.0871 37.0 37.0 37.0 25.0 37.0 125-129 34.807 37.0 37.0 37.0 25.0 37.0 130-134 34.7172 37.0 37.0 37.0 25.0 37.0 135-139 34.6543 37.0 37.0 37.0 25.0 37.0 140-144 34.568 37.0 37.0 37.0 25.0 37.0 145-149 34.4362 37.0 37.0 37.0 25.0 37.0 150 33.97 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 2.0 20 2.0 21 0.0 22 4.0 23 4.0 24 6.0 25 14.0 26 18.0 27 12.0 28 27.0 29 29.0 30 40.0 31 44.0 32 95.0 33 136.0 34 396.0 35 1156.0 36 1983.0 37 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.924999999999997 24.825 19.950000000000003 28.299999999999997 2 24.85 26.25 33.725 15.174999999999999 3 19.950000000000003 27.224999999999998 34.725 18.099999999999998 4 21.55 29.475 29.299999999999997 19.675 5 23.325000000000003 27.3 30.4 18.975 6 18.975 33.4 29.599999999999998 18.025 7 19.075 21.224999999999998 41.8 17.9 8 21.6 22.675 32.800000000000004 22.925 9 22.400000000000002 20.424999999999997 35.175 22.0 10-14 23.849999999999998 26.455000000000002 29.330000000000002 20.365 15-19 24.205 24.85 30.630000000000003 20.315 20-24 24.795 23.57 30.709999999999997 20.925 25-29 24.135 23.43 31.669999999999998 20.765 30-34 24.555 22.919999999999998 31.595000000000002 20.93 35-39 24.11 24.63 30.159999999999997 21.099999999999998 40-44 22.400000000000002 25.729999999999997 30.665 21.205 45-49 22.7 27.42 28.84 21.04 50-54 22.53 25.665 28.904999999999998 22.900000000000002 55-59 23.31 24.279999999999998 29.720000000000002 22.689999999999998 60-64 22.48 24.825 30.099999999999998 22.595000000000002 65-69 24.104999999999997 23.94 29.715000000000003 22.24 70-74 23.24 25.165 30.8 20.794999999999998 75-79 24.69 24.18 30.209999999999997 20.919999999999998 80-84 24.955 25.169999999999998 28.84 21.035 85-89 25.28 25.095 27.400000000000002 22.225 90-94 23.32 24.935 29.78 21.965 95-99 24.104999999999997 24.46 29.185 22.25 100-104 25.505 22.685 30.805 21.005 105-109 25.75 23.035 29.82 21.395 110-114 24.169999999999998 22.965 29.925 22.939999999999998 115-119 24.32 24.52 29.799999999999997 21.36 120-124 24.525 25.11 28.804999999999996 21.560000000000002 125-129 22.205 25.53 30.354999999999997 21.91 130-134 22.725 26.72 28.605000000000004 21.95 135-139 23.45 25.759999999999998 29.65 21.14 140-144 23.105 23.84 31.369999999999997 21.685 145-149 22.05 23.73 31.95 22.27 150 23.7 23.65 29.475 23.175 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 0.5 21 1.0 22 1.5 23 4.0 24 7.5 25 7.5 26 8.5 27 9.5 28 15.0 29 19.0 30 20.5 31 20.0 32 27.5 33 44.5 34 62.0 35 83.5 36 122.0 37 218.5 38 264.0 39 213.5 40 218.0 41 274.5 42 269.5 43 246.0 44 242.5 45 203.0 46 176.5 47 141.5 48 97.0 49 76.5 50 53.0 51 43.5 52 38.5 53 35.5 54 33.0 55 29.0 56 32.0 57 35.5 58 33.0 59 28.0 60 27.5 61 27.0 62 33.5 63 57.5 64 76.0 65 64.5 66 36.0 67 23.0 68 25.5 69 25.5 70 24.5 71 25.0 72 18.0 73 10.0 74 10.5 75 11.5 76 11.5 77 11.0 78 6.0 79 2.5 80 3.0 81 3.0 82 2.5 83 2.0 84 0.5 85 0.0 86 0.0 87 1.0 88 1.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.324999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 78.53991175290814 48.949999999999996 2 12.073806658644203 15.049999999999999 3 3.6101083032490973 6.75 4 1.4039310068190936 3.5000000000000004 5 1.4841556357801846 4.625 6 0.8022462896109106 3.0 7 0.5615724027276374 2.45 8 0.24067388688327318 1.2 9 0.1604492579221821 0.8999999999999999 >10 1.083032490974729 11.1 >50 0.04011231448054552 2.475 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 99 2.475 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 39 0.975 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 37 0.9249999999999999 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 36 0.8999999999999999 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 21 0.525 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 19 0.475 No Hit TATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACC 18 0.44999999999999996 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 17 0.42500000000000004 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 17 0.42500000000000004 No Hit GCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGC 16 0.4 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 16 0.4 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 16 0.4 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 16 0.4 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 15 0.375 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 15 0.375 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 14 0.35000000000000003 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 14 0.35000000000000003 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 12 0.3 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 12 0.3 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 12 0.3 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 11 0.27499999999999997 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 11 0.27499999999999997 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 10 0.25 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 10 0.25 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 10 0.25 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 10 0.25 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 10 0.25 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 10 0.25 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 9 0.22499999999999998 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 9 0.22499999999999998 No Hit AATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTAT 9 0.22499999999999998 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 9 0.22499999999999998 No Hit TGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGA 8 0.2 No Hit AAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAG 8 0.2 No Hit GATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTC 8 0.2 No Hit ATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATAT 8 0.2 No Hit TATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCAT 8 0.2 No Hit TGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACT 8 0.2 No Hit GGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGGCCTGGTCT 7 0.17500000000000002 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 7 0.17500000000000002 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 7 0.17500000000000002 No Hit TATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTTTT 7 0.17500000000000002 No Hit ATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGA 7 0.17500000000000002 No Hit CTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTA 7 0.17500000000000002 No Hit CTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAG 7 0.17500000000000002 No Hit AATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGC 7 0.17500000000000002 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 7 0.17500000000000002 No Hit TTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGG 7 0.17500000000000002 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 7 0.17500000000000002 No Hit CTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAA 7 0.17500000000000002 No Hit TACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGG 7 0.17500000000000002 No Hit GAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTT 7 0.17500000000000002 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 6 0.15 No Hit AAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATC 6 0.15 No Hit ATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGC 6 0.15 No Hit CAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGT 6 0.15 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 6 0.15 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 6 0.15 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 6 0.15 No Hit CAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCT 6 0.15 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 6 0.15 No Hit AATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTAT 6 0.15 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 6 0.15 No Hit GTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACT 6 0.15 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 6 0.15 No Hit TGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTC 6 0.15 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 6 0.15 No Hit GGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATC 6 0.15 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 6 0.15 No Hit CTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTAC 6 0.15 No Hit GGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACA 6 0.15 No Hit AGCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAA 6 0.15 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 5 0.125 No Hit CTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGT 5 0.125 No Hit GAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGAC 5 0.125 No Hit GCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAA 5 0.125 No Hit ATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATA 5 0.125 No Hit CTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCT 5 0.125 No Hit TGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCAT 5 0.125 No Hit TGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATA 5 0.125 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 5 0.125 No Hit GAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGC 5 0.125 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 5 0.125 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 5 0.125 No Hit TGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTC 5 0.125 No Hit CTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATT 5 0.125 No Hit AGCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTG 5 0.125 No Hit ACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGA 5 0.125 No Hit AGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAG 5 0.125 No Hit GGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTT 5 0.125 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 5 0.125 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 5 0.125 No Hit TGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTG 5 0.125 No Hit GGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTA 5 0.125 No Hit GGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGT 5 0.125 No Hit AACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAAT 5 0.125 No Hit CTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGC 5 0.125 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 5 0.125 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 5 0.125 No Hit TCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGA 5 0.125 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 5 0.125 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 5 0.125 No Hit TATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTA 5 0.125 No Hit AATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTA 5 0.125 No Hit CAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTC 5 0.125 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 5 0.125 No Hit AACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGG 5 0.125 No Hit GTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATATTATC 5 0.125 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.025 0.0 0.0 0.0 0.0 92-93 0.025 0.0 0.0 0.0 0.0 94-95 0.025 0.0 0.0 0.0 0.0 96-97 0.05 0.0 0.0 0.0 0.0 98-99 0.075 0.0 0.0 0.0 0.0 100-101 0.075 0.0 0.0 0.0 0.0 102-103 0.075 0.0 0.0 0.0 0.0 104-105 0.075 0.0 0.0 0.0 0.0 106-107 0.075 0.0 0.0 0.0 0.0 108-109 0.075 0.0 0.0 0.0 0.0 110-111 0.075 0.0 0.0 0.0 0.0 112-113 0.075 0.0 0.0 0.0 0.0 114-115 0.1 0.0 0.0 0.0 0.0 116-117 0.1 0.0 0.0 0.0 0.0 118-119 0.125 0.0 0.0 0.0 0.0 120-121 0.125 0.0 0.0 0.0 0.0 122-123 0.125 0.0 0.0 0.0 0.0 124-125 0.125 0.0 0.0 0.0 0.0 126-127 0.125 0.0 0.0 0.0 0.0 128-129 0.125 0.0 0.0 0.0 0.0 130-131 0.175 0.0 0.0 0.0 0.0 132-133 0.175 0.0 0.0 0.0 0.0 134-135 0.175 0.0 0.0 0.0 0.0 136-137 0.175 0.0 0.0 0.0 0.0 138 0.175 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGAGCT 10 0.006973645 144.0 6 CGAGAGC 10 0.006973645 144.0 5 >>END_MODULE Read 2792597 spots for ERR11006577.sra Written 2792597 spots for ERR11006577.sra Read 2792597 spots for ERR11006577.sra Written 2792597 spots for ERR11006577.sra Read 2792597 spots for ERR11006577.sra Written 2792597 spots for ERR11006577.sra Read 2792597 spots for ERR11006577.sra Written 2792597 spots for ERR11006577.sra Read 2792597 spots for ERR11006577.sra Written 2792597 spots for ERR11006577.sra Read 2792597 spots for ERR11006577.sra Written 2792597 spots for ERR11006577.sra Read 2792597 spots for ERR11006577.sra Written 2792597 spots for ERR11006577.sra Read 2792615 spots for ERR11006577.sra Written 2792615 spots for ERR11006577.sra Read 2792597 spots for ERR11006577.sra Written 2792597 spots for ERR11006577.sra Read 2792597 spots for ERR11006577.sra Written 2792597 spots for ERR11006577.sra Read 2792597 spots for ERR11006577.sra Written 2792597 spots for ERR11006577.sra Read 2792597 spots for ERR11006577.sra Written 2792597 spots for ERR11006577.sra Read 2792597 spots for ERR11006577.sra Written 2792597 spots for ERR11006577.sra Read 2792597 spots for ERR11006577.sra Written 2792597 spots for ERR11006577.sra Read 2792597 spots for ERR11006577.sra Written 2792597 spots for ERR11006577.sra Read 2792597 spots for ERR11006577.sra Written 2792597 spots for ERR11006577.sra Read 2792597 spots for ERR11006577.sra Written 2792597 spots for ERR11006577.sra Read 2792597 spots for ERR11006577.sra Written 2792597 spots for ERR11006577.sra Read 2792597 spots for ERR11006577.sra Written 2792597 spots for ERR11006577.sra Read 2792597 spots for ERR11006577.sra Written 2792597 spots for ERR11006577.sra SRR ids: ['ERR11006577.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_i4pukbwx ERR11006577.sra spots: 55851958 blocks: [[1, 2792597], [2792598, 5585194], [5585195, 8377791], [8377792, 11170388], [11170389, 13962985], [13962986, 16755582], [16755583, 19548179], [19548180, 22340776], [22340777, 25133373], [25133374, 27925970], [27925971, 30718567], [30718568, 33511164], [33511165, 36303761], [36303762, 39096358], [39096359, 41888955], [41888956, 44681552], [44681553, 47474149], [47474150, 50266746], [50266747, 53059343], [53059344, 55851958]] ERR11006577 file size 20523461 ERR11006577 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006577 ERR11006577_1.fastq ERR11006577_2.fastq Input file: ERR11006577_1.fastq Paired file: ERR11006577_2.fastq trimmed: ERR11006577-trimmed-pair1.fastq, ERR11006577-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 17:55:05 2024 >> started Fri Dec 6 17:58:10 2024 >> done (184.425s) 55851958 read pairs processed; of these: 131 ( 0.00%) short read pairs filtered out after trimming by size control 535 ( 0.00%) empty read pairs filtered out after trimming by size control 55851292 (100.00%) read pairs available; of these: 110951 ( 0.20%) trimmed read pairs available after processing 55740341 (99.80%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 14 0.00% 19 12 0.00% 20 13 0.00% 21 20 0.00% 22 41 0.00% 23 17 0.00% 24 15 0.00% 25 19 0.00% 26 13 0.00% 27 14 0.00% 28 29 0.00% 29 24 0.00% 30 43 0.00% 31 37 0.00% 32 22 0.00% 33 29 0.00% 34 25 0.00% 35 35 0.00% 36 13 0.00% 37 26 0.00% 38 31 0.00% 39 28 0.00% 40 31 0.00% 41 36 0.00% 42 49 0.00% 43 45 0.00% 44 33 0.00% 45 43 0.00% 46 41 0.00% 47 37 0.00% 48 52 0.00% 49 64 0.00% 50 66 0.00% 51 63 0.00% 52 78 0.00% 53 86 0.00% 54 80 0.00% 55 72 0.00% 56 64 0.00% 57 91 0.00% 58 86 0.00% 59 102 0.00% 60 100 0.00% 61 83 0.00% 62 97 0.00% 63 99 0.00% 64 104 0.00% 65 113 0.00% 66 122 0.00% 67 133 0.00% 68 112 0.00% 69 116 0.00% 70 130 0.00% 71 131 0.00% 72 152 0.00% 73 172 0.00% 74 179 0.00% 75 166 0.00% 76 162 0.00% 77 189 0.00% 78 202 0.00% 79 198 0.00% 80 213 0.00% 81 243 0.00% 82 207 0.00% 83 260 0.00% 84 265 0.00% 85 279 0.00% 86 268 0.00% 87 313 0.00% 88 299 0.00% 89 334 0.00% 90 366 0.00% 91 400 0.00% 92 409 0.00% 93 435 0.00% 94 402 0.00% 95 440 0.00% 96 461 0.00% 97 466 0.00% 98 553 0.00% 99 511 0.00% 100 606 0.00% 101 543 0.00% 102 611 0.00% 103 607 0.00% 104 716 0.00% 105 694 0.00% 106 750 0.00% 107 748 0.00% 108 797 0.00% 109 826 0.00% 110 837 0.00% 111 894 0.00% 112 1007 0.00% 113 1070 0.00% 114 1084 0.00% 115 1099 0.00% 116 1252 0.00% 117 1262 0.00% 118 1301 0.00% 119 1378 0.00% 120 1412 0.00% 121 1568 0.00% 122 1582 0.00% 123 1770 0.00% 124 1806 0.00% 125 2061 0.00% 126 1843 0.00% 127 1651 0.00% 128 1803 0.00% 129 2060 0.00% 130 2205 0.00% 131 2212 0.00% 132 2473 0.00% 133 2291 0.00% 134 2461 0.00% 135 2420 0.00% 136 2487 0.00% 137 2513 0.00% 138 2674 0.00% 139 2921 0.01% 140 2914 0.01% 141 3195 0.01% 142 3387 0.01% 143 3512 0.01% 144 3749 0.01% 145 3782 0.01% 146 4772 0.01% 147 4358 0.01% 148 4542 0.01% 149 4822 0.01% 150 55740341 99.80% 55851292 reads passed initial QC criterion=sequence-density sequence-density=0.26 sequence-density-rank=1 fanout-score=0.00 fanout-score-rank=33 prefix-density=0.00 prefix-fanout=1.0 sequence=GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTTATCCTTCCACCGTTGGAAGCGGGCAGTTGTCGCTGCTCTGTGAAGCCAGCCTCACGCTGTGCCTGCCAACATTATGGGCCGCGAAGCCTAGCTTTCGCTTAAGCTCCAACGGCCCACTACGCAACTTGGAACGGGCGGGCCATCAGTAGCACACCCAGACCAGGCCCGCAGCGCTACGGCAACGTCCACACCACCCTTAAAGCCCC criterion=fanout-score sequence-density=0.01 sequence-density-rank=37 fanout-score=64.15 fanout-score-rank=1 prefix-density=0.22 prefix-fanout=1.9 sequence=AAAAACAGTAGAAGTAGAACAGGTATAAATAAGAAAATCTTAGTTAAGAGGGTTCATGTAAAGAACAGGTTCTAAATCACGATCGATTCCCTTTTCAAAACCTGCTGCAGCAGCTCGGGCTCTTCCTGCATGCCACAAATGGCCCACAAAAAAGAAGAATCCTAGAACAAAATGAGAAGTCGATAACCAACTTCTAGGAGAGACATAATTAACTGCATTGATCTCGGTAGCTACGCCACCCACGGAATTTAAAGAGCCTAAAGGAGCATGGGTCATATATTCCGCTGAACGTCGTTCTTGCCAAGGTTGTATGTCTTTTTTCAACCTACTCAAGTCCAAACCGTTGGGCCCCCTTAGAGGTTCTAACCATGGAGCACGGAGGTCCCAAAAACGCATAGTTTCCCCTCCAAAGATAACCTCTCCCGTTGGGGAACGCATTAGATATTTACCTAAACCTGTGGGTCCTTGAGCAGATCCCACATTAGCTCCAAGACGCTGGTCTCTAACTAGA criterion=sequence-density sequence-density=0.30 sequence-density-rank=1 fanout-score=2.53 fanout-score-rank=27 prefix-density=0.38 prefix-fanout=2.0 sequence=TAGTCTTTACAT criterion=fanout-score sequence-density=0.12 sequence-density-rank=16 fanout-score=106.23 fanout-score-rank=1 prefix-density=12.08 prefix-fanout=1.0 sequence=CAACTGGATAAATAGCACTGAAA ERR11006577 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 17:58:47 Started mapping on | Dec 06 17:58:47 Finished on | Dec 06 18:04:50 Mapping speed, Million of reads per hour | 553.90 Number of input reads | 55851292 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 44393571 Uniquely mapped reads % | 79.49% Average mapped length | 298.69 Number of splices: Total | 12929820 Number of splices: Annotated (sjdb) | 11958929 Number of splices: GT/AG | 12599703 Number of splices: GC/AG | 132491 Number of splices: AT/AC | 21146 Number of splices: Non-canonical | 176480 Mismatch rate per base, % | 0.33% Deletion rate per base | 0.01% Deletion average length | 1.72 Insertion rate per base | 0.01% Insertion average length | 2.03 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 9851367 % of reads mapped to multiple loci | 17.64% Number of reads mapped to too many loci | 5657 % of reads mapped to too many loci | 0.01% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.74% % of reads unmapped: other | 0.12% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1606354 1606354 1606354 N_multimapping 9851367 9851367 9851367 N_noFeature 11276355 40946365 13677012 N_ambiguous 1720815 56359 667877 UnstrandedReadsAssigned:31396401 PositiveStrandReadsAssigned:3390847 NegativeStrandReadsAssigned:30048682 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006577 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006577-trimmed-pair1.fastq ERR11006577-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 55,851,292 reads, 31,591,327 reads pseudoaligned [quant] estimated average fragment length: 315.725 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,270 rounds 52973 ERR11006577.ke.tsv 35125 ERR11006577.se.tsv 88098 total ==> ERR11006577.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 621.687 0 0 PNS24247 1044 729.275 19.608 0.67021 PNS24249 1928 1613.27 147.685 2.28191 PNS24246 1044 729.275 19.608 0.67021 PNS24248 1044 729.275 19.608 0.67021 PNS24244 1471 1156.27 39.4907 0.851339 PNS24243 293 40.0518 0 0 KQK14069 1603 1288.27 3852.74 74.547 KQK14071 474 164.518 13.8799 2.10301 ==> ERR11006577.se.tsv <== BRADI_1g14170v3 4172 BRADI_1g53295v3 958 BRADI_1g59795v3 309 BRADI_1g07683v3 0 BRADI_1g00485v3 3 BRADI_1g20270v3 105 BRADI_1g74790v3 259 BRADI_1g09890v3 0 BRADI_1g77505v3 93 BRADI_1g48960v3 0 ERR11006577 completed mapping pipeline successfully