Starting /dee2/code/volunteer_pipeline.sh ERR11006578 current disk space = 1550711652352 free memory = 1596438564 ERR11006578 SRAfilesize 2646033214c36c6e8c7800c88d45fb73 ERR11006578.sra ERR11006578.sra file validated ERR11006578 is paired end ERR11006578 is conventional basespace ERR11006578 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006578_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.4035 37.0 37.0 37.0 37.0 37.0 2 36.301 37.0 37.0 37.0 37.0 37.0 3 36.3785 37.0 37.0 37.0 37.0 37.0 4 36.5285 37.0 37.0 37.0 37.0 37.0 5 36.481 37.0 37.0 37.0 37.0 37.0 6 36.4555 37.0 37.0 37.0 37.0 37.0 7 36.4295 37.0 37.0 37.0 37.0 37.0 8 36.4095 37.0 37.0 37.0 37.0 37.0 9 36.5535 37.0 37.0 37.0 37.0 37.0 10-14 36.4852 37.0 37.0 37.0 37.0 37.0 15-19 36.5392 37.0 37.0 37.0 37.0 37.0 20-24 36.4808 37.0 37.0 37.0 37.0 37.0 25-29 36.4486 37.0 37.0 37.0 37.0 37.0 30-34 36.388099999999994 37.0 37.0 37.0 37.0 37.0 35-39 36.3226 37.0 37.0 37.0 37.0 37.0 40-44 36.3146 37.0 37.0 37.0 37.0 37.0 45-49 36.299 37.0 37.0 37.0 37.0 37.0 50-54 36.3125 37.0 37.0 37.0 37.0 37.0 55-59 36.2225 37.0 37.0 37.0 37.0 37.0 60-64 36.1155 37.0 37.0 37.0 37.0 37.0 65-69 36.1851 37.0 37.0 37.0 37.0 37.0 70-74 36.2062 37.0 37.0 37.0 37.0 37.0 75-79 36.1662 37.0 37.0 37.0 37.0 37.0 80-84 36.143899999999995 37.0 37.0 37.0 37.0 37.0 85-89 36.0892 37.0 37.0 37.0 37.0 37.0 90-94 35.9375 37.0 37.0 37.0 37.0 37.0 95-99 35.9972 37.0 37.0 37.0 37.0 37.0 100-104 35.93489999999999 37.0 37.0 37.0 37.0 37.0 105-109 35.9799 37.0 37.0 37.0 37.0 37.0 110-114 35.842600000000004 37.0 37.0 37.0 37.0 37.0 115-119 35.9209 37.0 37.0 37.0 37.0 37.0 120-124 35.7658 37.0 37.0 37.0 37.0 37.0 125-129 35.7836 37.0 37.0 37.0 37.0 37.0 130-134 35.7798 37.0 37.0 37.0 37.0 37.0 135-139 35.5052 37.0 37.0 37.0 37.0 37.0 140-144 35.5925 37.0 37.0 37.0 37.0 37.0 145-149 35.3312 37.0 37.0 37.0 34.6 37.0 150 35.4735 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 1.0 22 0.0 23 1.0 24 3.0 25 5.0 26 3.0 27 7.0 28 18.0 29 21.0 30 29.0 31 36.0 32 66.0 33 82.0 34 141.0 35 400.0 36 3028.0 37 158.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.925 12.0 13.100000000000001 35.975 2 26.988494247123562 9.204602301150576 27.188594297148573 36.61830915457729 3 21.7 11.924999999999999 29.25 37.125 4 28.775000000000002 14.75 25.874999999999996 30.599999999999998 5 27.675 18.45 27.500000000000004 26.375 6 24.175 28.275 22.975 24.575 7 17.675 28.349999999999998 34.825 19.15 8 16.35 27.975 35.699999999999996 19.975 9 16.950000000000003 25.025 37.6 20.424999999999997 10-14 19.875 31.415 27.175 21.535 15-19 19.555 34.405 25.195 20.845 20-24 18.855 29.24 28.48 23.425 25-29 21.759999999999998 31.655 25.740000000000002 20.845 30-34 23.98 30.475 25.305 20.24 35-39 22.509999999999998 31.419999999999998 25.905 20.165 40-44 20.155 29.970000000000002 25.929999999999996 23.945 45-49 20.025000000000002 28.585 27.950000000000003 23.44 50-54 20.635 32.065 25.955000000000002 21.345 55-59 22.33 29.535 23.155 24.98 60-64 19.755 31.879999999999995 25.169999999999998 23.195 65-69 21.13 29.785 25.335 23.75 70-74 22.945 29.86 21.645 25.55 75-79 22.505 29.544999999999998 25.169999999999998 22.78 80-84 22.545 29.675 24.545 23.235 85-89 24.345 28.88 25.14 21.634999999999998 90-94 21.525 30.130000000000003 27.0 21.345 95-99 23.849999999999998 29.735 22.66 23.755000000000003 100-104 21.82 31.005 24.165 23.01 105-109 21.695 28.465 26.66 23.18 110-114 24.215 27.625 24.965 23.195 115-119 19.82 30.659999999999997 25.5 24.02 120-124 20.225 29.935000000000002 24.935 24.905 125-129 20.885 31.78 22.195 25.14 130-134 23.06 30.445 24.955 21.54 135-139 23.400000000000002 29.705 23.21 23.685000000000002 140-144 24.63 28.405 26.25 20.715 145-149 23.11 30.209999999999997 25.419999999999998 21.26 150 24.85 26.575 26.924999999999997 21.65 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 0.0 20 1.5 21 2.5 22 1.5 23 1.5 24 3.5 25 5.5 26 4.5 27 6.5 28 11.5 29 15.5 30 21.0 31 20.5 32 22.5 33 34.0 34 44.0 35 52.5 36 78.5 37 150.0 38 267.0 39 299.0 40 263.0 41 279.5 42 280.5 43 264.0 44 233.5 45 210.0 46 206.5 47 181.0 48 124.5 49 84.5 50 75.5 51 48.5 52 44.0 53 42.5 54 28.5 55 27.0 56 28.0 57 27.5 58 25.5 59 22.0 60 19.5 61 23.0 62 28.5 63 28.5 64 45.0 65 61.0 66 43.0 67 24.5 68 27.0 69 26.5 70 20.0 71 19.5 72 20.0 73 15.0 74 8.0 75 9.5 76 8.5 77 6.0 78 5.5 79 4.5 80 4.0 81 2.5 82 2.5 83 2.5 84 0.5 85 0.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.05 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.324999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 80.64649813510152 48.65 2 12.101118939079983 14.6 3 3.0667219229175298 5.55 4 0.8288437629506838 2.0 5 0.6216328222130129 1.875 6 0.4144218814753419 1.5 7 0.33153750518027353 1.4000000000000001 8 0.24865312888520513 1.2 9 0.20721094073767096 1.125 >10 1.4504765851636967 17.349999999999998 >50 0.04144218814753419 1.275 >100 0.04144218814753419 3.4750000000000005 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 139 3.4750000000000005 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 51 1.275 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 40 1.0 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 40 1.0 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 40 1.0 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 33 0.8250000000000001 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 33 0.8250000000000001 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 31 0.775 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 31 0.775 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 27 0.675 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 24 0.6 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 23 0.575 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 22 0.5499999999999999 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 22 0.5499999999999999 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 22 0.5499999999999999 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 21 0.525 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 20 0.5 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 19 0.475 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 17 0.42500000000000004 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 16 0.4 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 16 0.4 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 15 0.375 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 14 0.35000000000000003 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 14 0.35000000000000003 No Hit TGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCT 14 0.35000000000000003 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 14 0.35000000000000003 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 13 0.325 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 13 0.325 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 12 0.3 No Hit ACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 12 0.3 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 12 0.3 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 11 0.27499999999999997 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 11 0.27499999999999997 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 11 0.27499999999999997 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 11 0.27499999999999997 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 10 0.25 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 10 0.25 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 9 0.22499999999999998 No Hit GCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCC 9 0.22499999999999998 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 9 0.22499999999999998 No Hit ATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATA 9 0.22499999999999998 No Hit TAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGA 9 0.22499999999999998 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 8 0.2 No Hit CTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAG 8 0.2 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 8 0.2 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 8 0.2 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 8 0.2 No Hit TCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTT 8 0.2 No Hit GCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAA 7 0.17500000000000002 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 7 0.17500000000000002 No Hit CCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTT 7 0.17500000000000002 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 7 0.17500000000000002 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 7 0.17500000000000002 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 7 0.17500000000000002 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 7 0.17500000000000002 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 7 0.17500000000000002 No Hit CGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 6 0.15 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 6 0.15 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 6 0.15 No Hit TCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGT 6 0.15 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 6 0.15 No Hit CCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAA 6 0.15 No Hit CGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAAC 6 0.15 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 6 0.15 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 6 0.15 No Hit GGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGG 6 0.15 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 5 0.125 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 5 0.125 No Hit CAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAA 5 0.125 No Hit GGGACCAAAAGCGATTAGCAGTGACCATTGAATAAGTTTCTTCAGCTTGA 5 0.125 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 5 0.125 No Hit TGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGA 5 0.125 No Hit GGAGCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACT 5 0.125 No Hit GGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 5 0.125 No Hit GGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAG 5 0.125 No Hit CGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCATAC 5 0.125 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 5 0.125 No Hit CTCCTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGAT 5 0.125 No Hit GCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAGCTGCAA 5 0.125 No Hit GTCGTGAATAGCTCCGTGGAATAAAATAGAATTTCCTTATGCATAGAACT 5 0.125 No Hit AACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0125 0.0 0.0 0.0 0.0 98-99 0.025 0.0 0.0 0.0 0.0 100-101 0.05 0.0 0.0 0.0 0.0 102-103 0.05 0.0 0.0 0.0 0.0 104-105 0.05 0.0 0.0 0.0 0.0 106-107 0.05 0.0 0.0 0.0 0.0 108-109 0.05 0.0 0.0 0.0 0.0 110-111 0.05 0.0 0.0 0.0 0.0 112-113 0.05 0.0 0.0 0.0 0.0 114-115 0.05 0.0 0.0 0.0 0.0 116-117 0.1125 0.0 0.0 0.0 0.0 118-119 0.16249999999999998 0.0 0.0 0.0 0.0 120-121 0.175 0.0 0.0 0.0 0.0 122-123 0.175 0.0 0.0 0.0 0.0 124-125 0.225 0.0 0.0 0.0 0.0 126-127 0.225 0.0 0.0 0.0 0.0 128-129 0.225 0.0 0.0 0.0 0.0 130-131 0.225 0.0 0.0 0.0 0.0 132-133 0.225 0.0 0.0 0.0 0.0 134-135 0.225 0.0 0.0 0.0 0.0 136-137 0.2625 0.0 0.0 0.0 0.0 138 0.275 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCGCTG 10 0.006973645 144.0 2 TCGCTGG 10 0.006973645 144.0 3 GTTCGCT 10 0.006973645 144.0 1 >>END_MODULE ERR11006578 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006578_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0975 37.0 37.0 37.0 37.0 37.0 2 36.054 37.0 37.0 37.0 37.0 37.0 3 36.2325 37.0 37.0 37.0 37.0 37.0 4 36.084 37.0 37.0 37.0 37.0 37.0 5 36.1555 37.0 37.0 37.0 37.0 37.0 6 36.165 37.0 37.0 37.0 37.0 37.0 7 36.08 37.0 37.0 37.0 37.0 37.0 8 36.2585 37.0 37.0 37.0 37.0 37.0 9 36.139 37.0 37.0 37.0 37.0 37.0 10-14 36.2047 37.0 37.0 37.0 37.0 37.0 15-19 36.2143 37.0 37.0 37.0 37.0 37.0 20-24 36.1787 37.0 37.0 37.0 37.0 37.0 25-29 36.0893 37.0 37.0 37.0 37.0 37.0 30-34 36.0752 37.0 37.0 37.0 37.0 37.0 35-39 36.045300000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.0864 37.0 37.0 37.0 37.0 37.0 45-49 36.0455 37.0 37.0 37.0 37.0 37.0 50-54 35.9719 37.0 37.0 37.0 37.0 37.0 55-59 35.842699999999994 37.0 37.0 37.0 37.0 37.0 60-64 35.922000000000004 37.0 37.0 37.0 37.0 37.0 65-69 35.7691 37.0 37.0 37.0 37.0 37.0 70-74 35.76440000000001 37.0 37.0 37.0 37.0 37.0 75-79 35.7771 37.0 37.0 37.0 37.0 37.0 80-84 35.698800000000006 37.0 37.0 37.0 37.0 37.0 85-89 35.6318 37.0 37.0 37.0 37.0 37.0 90-94 35.545 37.0 37.0 37.0 37.0 37.0 95-99 35.3817 37.0 37.0 37.0 34.6 37.0 100-104 35.394999999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.3051 37.0 37.0 37.0 27.4 37.0 110-114 35.2856 37.0 37.0 37.0 29.8 37.0 115-119 35.104 37.0 37.0 37.0 25.0 37.0 120-124 35.341300000000004 37.0 37.0 37.0 34.6 37.0 125-129 35.113099999999996 37.0 37.0 37.0 27.4 37.0 130-134 34.992200000000004 37.0 37.0 37.0 25.0 37.0 135-139 34.863800000000005 37.0 37.0 37.0 25.0 37.0 140-144 34.741099999999996 37.0 37.0 37.0 25.0 37.0 145-149 34.810199999999995 37.0 37.0 37.0 25.0 37.0 150 34.552 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 0.0 16 0.0 17 1.0 18 0.0 19 0.0 20 0.0 21 0.0 22 2.0 23 2.0 24 4.0 25 5.0 26 10.0 27 15.0 28 17.0 29 30.0 30 28.0 31 55.0 32 55.0 33 137.0 34 317.0 35 997.0 36 2283.0 37 41.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.675 26.0 18.15 28.175 2 23.474999999999998 24.175 35.949999999999996 16.400000000000002 3 19.875 27.250000000000004 33.925 18.95 4 24.625 27.474999999999998 28.000000000000004 19.900000000000002 5 23.474999999999998 29.675 29.15 17.7 6 19.575 32.9 28.475 19.05 7 18.2 21.45 41.85 18.5 8 22.75 22.5 30.975 23.775 9 22.2 19.375 35.625 22.8 10-14 23.21 26.8 29.110000000000003 20.880000000000003 15-19 23.775 25.869999999999997 30.035 20.32 20-24 25.4 23.225 30.285 21.09 25-29 23.86 23.995 30.880000000000003 21.265 30-34 24.145 24.45 30.104999999999997 21.3 35-39 23.89 24.57 30.285 21.255 40-44 22.869999999999997 26.44 30.245 20.445 45-49 22.465 27.310000000000002 28.51 21.715 50-54 22.29 26.6 28.895 22.215 55-59 22.595000000000002 25.105 29.86 22.439999999999998 60-64 22.28 25.259999999999998 28.975 23.485 65-69 23.494999999999997 24.529999999999998 29.64 22.335 70-74 23.72 24.87 30.5 20.91 75-79 23.575 24.82 30.630000000000003 20.974999999999998 80-84 24.41 25.330000000000002 28.985 21.275 85-89 24.615000000000002 25.41 27.62 22.355 90-94 23.255 25.569999999999997 28.845 22.33 95-99 22.965 24.8 29.330000000000002 22.905 100-104 24.85 23.080000000000002 30.575000000000003 21.495 105-109 25.645 22.695 29.755 21.905 110-114 23.880000000000003 23.995 30.080000000000002 22.045 115-119 23.935000000000002 24.91 29.39 21.765 120-124 24.64 25.05 28.155 22.155 125-129 23.044999999999998 25.679999999999996 29.62 21.654999999999998 130-134 23.064999999999998 26.44 28.64 21.855 135-139 23.565 25.525 30.240000000000002 20.669999999999998 140-144 22.79 24.610000000000003 30.245 22.355 145-149 22.42 23.825 31.540000000000003 22.215 150 22.575 22.900000000000002 30.475 24.05 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.5 21 3.0 22 3.0 23 3.0 24 3.0 25 9.0 26 15.0 27 15.5 28 21.5 29 28.0 30 39.0 31 39.5 32 35.0 33 45.0 34 52.5 35 76.5 36 124.5 37 214.5 38 254.0 39 223.0 40 229.5 41 245.0 42 231.0 43 219.0 44 227.0 45 206.0 46 179.0 47 146.0 48 107.0 49 77.5 50 57.5 51 55.0 52 46.0 53 37.5 54 31.5 55 26.5 56 25.5 57 23.5 58 29.0 59 40.0 60 36.0 61 30.0 62 28.5 63 51.5 64 65.5 65 54.0 66 40.0 67 26.5 68 32.5 69 32.5 70 26.0 71 24.5 72 24.5 73 21.0 74 16.5 75 12.5 76 5.5 77 4.5 78 7.0 79 7.0 80 3.0 81 0.5 82 1.0 83 0.5 84 1.0 85 1.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.85 #Duplication Level Percentage of deduplicated Percentage of total 1 78.73955960516325 51.849999999999994 2 12.794229309035687 16.85 3 3.416856492027335 6.75 4 1.518602885345482 4.0 5 1.2148823082763858 4.0 6 0.64540622627183 2.55 7 0.45558086560364464 2.1 8 0.26575550493545935 1.4000000000000001 9 0.18982536066818526 1.125 >10 0.721336370539104 7.449999999999999 >50 0.037965072133637055 1.925 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 77 1.925 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 29 0.7250000000000001 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 26 0.65 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 26 0.65 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 18 0.44999999999999996 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 18 0.44999999999999996 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 18 0.44999999999999996 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 16 0.4 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 16 0.4 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 15 0.375 No Hit TATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACC 14 0.35000000000000003 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 13 0.325 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 12 0.3 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 12 0.3 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 12 0.3 No Hit GTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCAT 12 0.3 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 11 0.27499999999999997 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 10 0.25 No Hit ATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATAT 10 0.25 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 10 0.25 No Hit AAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATC 9 0.22499999999999998 No Hit AGCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTG 9 0.22499999999999998 No Hit GAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCG 9 0.22499999999999998 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 9 0.22499999999999998 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 9 0.22499999999999998 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 8 0.2 No Hit AAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTT 8 0.2 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 8 0.2 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 8 0.2 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 8 0.2 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 8 0.2 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 8 0.2 No Hit CTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGA 7 0.17500000000000002 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 7 0.17500000000000002 No Hit GTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTAT 7 0.17500000000000002 No Hit ACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGT 7 0.17500000000000002 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 7 0.17500000000000002 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 7 0.17500000000000002 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 7 0.17500000000000002 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 7 0.17500000000000002 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 7 0.17500000000000002 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 7 0.17500000000000002 No Hit CTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAA 7 0.17500000000000002 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 7 0.17500000000000002 No Hit ACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAG 6 0.15 No Hit GTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCT 6 0.15 No Hit CAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCT 6 0.15 No Hit AATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTAT 6 0.15 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 6 0.15 No Hit ATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCT 6 0.15 No Hit CTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAG 6 0.15 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 6 0.15 No Hit CTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTT 6 0.15 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 6 0.15 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 6 0.15 No Hit TGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATA 6 0.15 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 6 0.15 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 6 0.15 No Hit CTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTAC 6 0.15 No Hit AGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATG 6 0.15 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 6 0.15 No Hit GCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAA 5 0.125 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 5 0.125 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 5 0.125 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 5 0.125 No Hit ATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCT 5 0.125 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 5 0.125 No Hit GGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTC 5 0.125 No Hit GATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTC 5 0.125 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 5 0.125 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 5 0.125 No Hit CTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATT 5 0.125 No Hit CAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCT 5 0.125 No Hit GTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGT 5 0.125 No Hit GGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTA 5 0.125 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 5 0.125 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 5 0.125 No Hit AGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCT 5 0.125 No Hit ATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATT 5 0.125 No Hit TGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATT 5 0.125 No Hit TCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGA 5 0.125 No Hit CTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCT 5 0.125 No Hit TCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACT 5 0.125 No Hit TATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTA 5 0.125 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 5 0.125 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 5 0.125 No Hit AGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTC 5 0.125 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 5 0.125 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 5 0.125 No Hit TCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTG 5 0.125 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 5 0.125 No Hit GTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGG 5 0.125 No Hit GCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACAC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0125 0.0 0.0 0.0 0.0 98-99 0.025 0.0 0.0 0.0 0.0 100-101 0.05 0.0 0.0 0.0 0.0 102-103 0.05 0.0 0.0 0.0 0.0 104-105 0.05 0.0 0.0 0.0 0.0 106-107 0.05 0.0 0.0 0.0 0.0 108-109 0.05 0.0 0.0 0.0 0.0 110-111 0.05 0.0 0.0 0.0 0.0 112-113 0.05 0.0 0.0 0.0 0.0 114-115 0.05 0.0 0.0 0.0 0.0 116-117 0.1125 0.0 0.0 0.0 0.0 118-119 0.16249999999999998 0.0 0.0 0.0 0.0 120-121 0.175 0.0 0.0 0.0 0.0 122-123 0.175 0.0 0.0 0.0 0.0 124-125 0.225 0.0 0.0 0.0 0.0 126-127 0.225 0.0 0.0 0.0 0.0 128-129 0.225 0.0 0.0 0.0 0.0 130-131 0.225 0.0 0.0 0.0 0.0 132-133 0.225 0.0 0.0 0.0 0.0 134-135 0.225 0.0 0.0 0.0 0.0 136-137 0.25 0.0 0.0 0.0 0.0 138 0.25 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATTTGA 30 0.0015031899 23.999998 40-44 >>END_MODULE Read 2469325 spots for ERR11006578.sra Written 2469325 spots for ERR11006578.sra Read 2469325 spots for ERR11006578.sra Written 2469325 spots for ERR11006578.sra Read 2469325 spots for ERR11006578.sra Written 2469325 spots for ERR11006578.sra Read 2469325 spots for ERR11006578.sra Written 2469325 spots for ERR11006578.sra Read 2469325 spots for ERR11006578.sra Written 2469325 spots for ERR11006578.sra Read 2469325 spots for ERR11006578.sra Written 2469325 spots for ERR11006578.sra Read 2469325 spots for ERR11006578.sra Written 2469325 spots for ERR11006578.sra Read 2469325 spots for ERR11006578.sra Written 2469325 spots for ERR11006578.sra Read 2469325 spots for ERR11006578.sra Written 2469325 spots for ERR11006578.sra Read 2469325 spots for ERR11006578.sra Written 2469325 spots for ERR11006578.sra Read 2469325 spots for ERR11006578.sra Written 2469325 spots for ERR11006578.sra Read 2469325 spots for ERR11006578.sra Written 2469325 spots for ERR11006578.sra Read 2469325 spots for ERR11006578.sra Written 2469325 spots for ERR11006578.sra Read 2469325 spots for ERR11006578.sra Written 2469325 spots for ERR11006578.sra Read 2469325 spots for ERR11006578.sra Written 2469325 spots for ERR11006578.sra Read 2469325 spots for ERR11006578.sra Written 2469325 spots for ERR11006578.sra Read 2469325 spots for ERR11006578.sra Written 2469325 spots for ERR11006578.sra Read 2469325 spots for ERR11006578.sra Written 2469325 spots for ERR11006578.sra Read 2469325 spots for ERR11006578.sra Written 2469325 spots for ERR11006578.sra Read 2469339 spots for ERR11006578.sra Written 2469339 spots for ERR11006578.sra SRR ids: ['ERR11006578.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_6gtfgki7 ERR11006578.sra spots: 49386514 blocks: [[1, 2469325], [2469326, 4938650], [4938651, 7407975], [7407976, 9877300], [9877301, 12346625], [12346626, 14815950], [14815951, 17285275], [17285276, 19754600], [19754601, 22223925], [22223926, 24693250], [24693251, 27162575], [27162576, 29631900], [29631901, 32101225], [32101226, 34570550], [34570551, 37039875], [37039876, 39509200], [39509201, 41978525], [41978526, 44447850], [44447851, 46917175], [46917176, 49386514]] ERR11006578 file size 18146470 ERR11006578 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006578 ERR11006578_1.fastq ERR11006578_2.fastq Input file: ERR11006578_1.fastq Paired file: ERR11006578_2.fastq trimmed: ERR11006578-trimmed-pair1.fastq, ERR11006578-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 17:55:02 2024 >> started Fri Dec 6 17:57:08 2024 >> done (126.253s) 49386514 read pairs processed; of these: 245 ( 0.00%) short read pairs filtered out after trimming by size control 451 ( 0.00%) empty read pairs filtered out after trimming by size control 49385818 (100.00%) read pairs available; of these: 109341 ( 0.22%) trimmed read pairs available after processing 49276477 (99.78%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 9 0.00% 19 11 0.00% 20 17 0.00% 21 25 0.00% 22 50 0.00% 23 18 0.00% 24 16 0.00% 25 32 0.00% 26 25 0.00% 27 15 0.00% 28 25 0.00% 29 29 0.00% 30 20 0.00% 31 40 0.00% 32 31 0.00% 33 34 0.00% 34 28 0.00% 35 41 0.00% 36 29 0.00% 37 42 0.00% 38 38 0.00% 39 44 0.00% 40 39 0.00% 41 47 0.00% 42 49 0.00% 43 58 0.00% 44 46 0.00% 45 46 0.00% 46 45 0.00% 47 63 0.00% 48 66 0.00% 49 51 0.00% 50 60 0.00% 51 65 0.00% 52 71 0.00% 53 79 0.00% 54 60 0.00% 55 68 0.00% 56 92 0.00% 57 71 0.00% 58 94 0.00% 59 76 0.00% 60 91 0.00% 61 101 0.00% 62 118 0.00% 63 93 0.00% 64 105 0.00% 65 129 0.00% 66 109 0.00% 67 133 0.00% 68 139 0.00% 69 150 0.00% 70 133 0.00% 71 151 0.00% 72 159 0.00% 73 161 0.00% 74 172 0.00% 75 184 0.00% 76 207 0.00% 77 178 0.00% 78 211 0.00% 79 189 0.00% 80 213 0.00% 81 238 0.00% 82 273 0.00% 83 244 0.00% 84 280 0.00% 85 296 0.00% 86 275 0.00% 87 321 0.00% 88 329 0.00% 89 373 0.00% 90 377 0.00% 91 416 0.00% 92 372 0.00% 93 410 0.00% 94 417 0.00% 95 478 0.00% 96 459 0.00% 97 542 0.00% 98 566 0.00% 99 532 0.00% 100 566 0.00% 101 611 0.00% 102 635 0.00% 103 685 0.00% 104 661 0.00% 105 768 0.00% 106 824 0.00% 107 795 0.00% 108 799 0.00% 109 828 0.00% 110 887 0.00% 111 977 0.00% 112 1064 0.00% 113 971 0.00% 114 1009 0.00% 115 1178 0.00% 116 1240 0.00% 117 1257 0.00% 118 1219 0.00% 119 1268 0.00% 120 1460 0.00% 121 1552 0.00% 122 1625 0.00% 123 1653 0.00% 124 1702 0.00% 125 1932 0.00% 126 1762 0.00% 127 1791 0.00% 128 1859 0.00% 129 2024 0.00% 130 2135 0.00% 131 2241 0.00% 132 2436 0.00% 133 2207 0.00% 134 2417 0.00% 135 2261 0.00% 136 2467 0.00% 137 2390 0.00% 138 2684 0.01% 139 2908 0.01% 140 2838 0.01% 141 3180 0.01% 142 3305 0.01% 143 3483 0.01% 144 3619 0.01% 145 3497 0.01% 146 4222 0.01% 147 4235 0.01% 148 4311 0.01% 149 4714 0.01% 150 49276477 99.78% 49385818 reads passed initial QC criterion=sequence-density sequence-density=0.38 sequence-density-rank=1 fanout-score=0.00 fanout-score-rank=32 prefix-density=0.00 prefix-fanout=1.0 sequence=TGCGGGAACTTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=67.45 fanout-score-rank=1 prefix-density=0.29 prefix-fanout=2.1 sequence=AAAAACAGTAGAAGTAGAACAGGTATAAATAAGAAAATCTTAGTTAAGAGGGTTCATGTAAAGAACAGGTTCTAAATCACGATCGATTCCCTTTTCAAAACCTGCTGCAGCAGCTCGGGCTCTTCCTGCATGCCACAAATGGCCCACAAAAAAGAAGAATCCTAGAACAAAATGAGAAGTCGATAACCAACTTCTAGGAGAGACATAATTAACTGCATTGATCTCGGTAGCTACGCCACCCACGGAATTTAAAGAGCCTAAAGGAGCATGGGTCATATATTCCGCTGAACGTCGTTCTTGCCAAGGTTGTATGTCTTTTTTCAACCTACTCAAGTCCAAACCGTTGGGCCCCCTTAGAGGTTCTAACCATGGAGCACGGAGGTCCCAAAAACGCATAGTTTCCCCTCCAAAGATAACCTCTCCCGTTGGGGAACGCATTAGATATTTACCTAAACCTGTGGGTCCTTGAGCAGATCCCACATTAGCTCCAAGACGCTGGTCTCTAACTAGA criterion=sequence-density sequence-density=0.29 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=31 prefix-density=0.28 prefix-fanout=2.0 sequence=GTTGAACAAGTA criterion=fanout-score sequence-density=0.09 sequence-density-rank=16 fanout-score=110.08 fanout-score-rank=1 prefix-density=9.93 prefix-fanout=1.0 sequence=CTGGATAACTATCACTGAAAATC ERR11006578 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 17:58:30 Started mapping on | Dec 06 17:58:30 Finished on | Dec 06 18:05:12 Mapping speed, Million of reads per hour | 442.26 Number of input reads | 49385818 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 38264559 Uniquely mapped reads % | 77.48% Average mapped length | 298.72 Number of splices: Total | 12823814 Number of splices: Annotated (sjdb) | 11895428 Number of splices: GT/AG | 12490039 Number of splices: GC/AG | 138490 Number of splices: AT/AC | 20838 Number of splices: Non-canonical | 174447 Mismatch rate per base, % | 0.31% Deletion rate per base | 0.01% Deletion average length | 1.76 Insertion rate per base | 0.01% Insertion average length | 2.04 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 9529212 % of reads mapped to multiple loci | 19.30% Number of reads mapped to too many loci | 6765 % of reads mapped to too many loci | 0.01% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.02% % of reads unmapped: other | 0.19% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1592047 1592047 1592047 N_multimapping 9529212 9529212 9529212 N_noFeature 9764710 35092422 11918630 N_ambiguous 1727169 50881 711963 UnstrandedReadsAssigned:26772680 PositiveStrandReadsAssigned:3121256 NegativeStrandReadsAssigned:25633966 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006578 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006578-trimmed-pair1.fastq ERR11006578-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 49,385,818 reads, 28,832,955 reads pseudoaligned [quant] estimated average fragment length: 318.365 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,115 rounds 52973 ERR11006578.ke.tsv 35125 ERR11006578.se.tsv 88098 total ==> ERR11006578.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 618.852 0 0 PNS24247 1044 726.635 26.842 1.04212 PNS24249 1928 1610.63 116.745 2.04484 PNS24246 1044 726.635 26.842 1.04212 PNS24248 1044 726.635 26.842 1.04212 PNS24244 1471 1153.63 33.7292 0.824817 PNS24243 293 40.3054 0 0 KQK14069 1603 1285.63 3035.86 66.6168 KQK14071 474 162.214 13.3248 2.31735 ==> ERR11006578.se.tsv <== BRADI_1g14170v3 3293 BRADI_1g53295v3 962 BRADI_1g59795v3 270 BRADI_1g07683v3 0 BRADI_1g00485v3 4 BRADI_1g20270v3 100 BRADI_1g74790v3 267 BRADI_1g09890v3 0 BRADI_1g77505v3 82 BRADI_1g48960v3 1 ERR11006578 completed mapping pipeline successfully