Starting /dee2/code/volunteer_pipeline.sh ERR11006579 current disk space = 1550710157312 free memory = 1599183004 ERR11006579 SRAfilesize 01ff252b64cd432449ad9055c30f9fae ERR11006579.sra ERR11006579.sra file validated ERR11006579 is paired end ERR11006579 is conventional basespace ERR11006579 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006579_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.4895 37.0 37.0 37.0 37.0 37.0 2 36.5115 37.0 37.0 37.0 37.0 37.0 3 36.647 37.0 37.0 37.0 37.0 37.0 4 36.5765 37.0 37.0 37.0 37.0 37.0 5 36.595 37.0 37.0 37.0 37.0 37.0 6 36.57 37.0 37.0 37.0 37.0 37.0 7 36.616 37.0 37.0 37.0 37.0 37.0 8 36.564 37.0 37.0 37.0 37.0 37.0 9 36.54 37.0 37.0 37.0 37.0 37.0 10-14 36.611200000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.6136 37.0 37.0 37.0 37.0 37.0 20-24 36.5689 37.0 37.0 37.0 37.0 37.0 25-29 36.4934 37.0 37.0 37.0 37.0 37.0 30-34 36.4713 37.0 37.0 37.0 37.0 37.0 35-39 36.464800000000004 37.0 37.0 37.0 37.0 37.0 40-44 36.482899999999994 37.0 37.0 37.0 37.0 37.0 45-49 36.50749999999999 37.0 37.0 37.0 37.0 37.0 50-54 36.453199999999995 37.0 37.0 37.0 37.0 37.0 55-59 36.419000000000004 37.0 37.0 37.0 37.0 37.0 60-64 36.333299999999994 37.0 37.0 37.0 37.0 37.0 65-69 36.42280000000001 37.0 37.0 37.0 37.0 37.0 70-74 36.3779 37.0 37.0 37.0 37.0 37.0 75-79 36.3743 37.0 37.0 37.0 37.0 37.0 80-84 36.3625 37.0 37.0 37.0 37.0 37.0 85-89 36.3301 37.0 37.0 37.0 37.0 37.0 90-94 36.236900000000006 37.0 37.0 37.0 37.0 37.0 95-99 36.35360000000001 37.0 37.0 37.0 37.0 37.0 100-104 36.2548 37.0 37.0 37.0 37.0 37.0 105-109 36.2222 37.0 37.0 37.0 37.0 37.0 110-114 36.2165 37.0 37.0 37.0 37.0 37.0 115-119 36.256099999999996 37.0 37.0 37.0 37.0 37.0 120-124 36.1565 37.0 37.0 37.0 37.0 37.0 125-129 36.1852 37.0 37.0 37.0 37.0 37.0 130-134 36.1443 37.0 37.0 37.0 37.0 37.0 135-139 36.0263 37.0 37.0 37.0 37.0 37.0 140-144 36.07655 37.0 37.0 37.0 37.0 37.0 145-149 35.954100000000004 37.0 37.0 37.0 37.0 37.0 150 35.8125 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 0.0 24 0.0 25 4.0 26 4.0 27 7.0 28 13.0 29 8.0 30 29.0 31 38.0 32 41.0 33 63.0 34 105.0 35 220.0 36 3035.0 37 432.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.15 12.8 14.399999999999999 34.65 2 29.175 9.049999999999999 25.525 36.25 3 21.425 11.475 30.25 36.85 4 27.500000000000004 14.399999999999999 27.150000000000002 30.95 5 28.275 17.05 29.125 25.55 6 24.15 28.325 21.6 25.924999999999997 7 17.224999999999998 28.675 33.975 20.125 8 16.175 28.249999999999996 35.675000000000004 19.900000000000002 9 15.925 25.4 38.6 20.075000000000003 10-14 19.48 31.2 28.27 21.05 15-19 19.08 36.295 24.349999999999998 20.275000000000002 20-24 18.07 29.375 29.685 22.869999999999997 25-29 21.785 31.805 26.395000000000003 20.015 30-34 24.57 30.25 25.740000000000002 19.439999999999998 35-39 22.830000000000002 32.379999999999995 25.025 19.765 40-44 19.915 30.79 25.75 23.544999999999998 45-49 19.36 29.160000000000004 28.249999999999996 23.23 50-54 20.125 32.675 26.640000000000004 20.560000000000002 55-59 22.895 29.89 23.294999999999998 23.919999999999998 60-64 19.925 32.295 24.23 23.549999999999997 65-69 20.145 30.135 25.365 24.355 70-74 23.53 30.995 20.875 24.6 75-79 23.01 29.085 25.974999999999998 21.93 80-84 21.905 29.544999999999998 25.39 23.16 85-89 25.915 29.020000000000003 24.104999999999997 20.96 90-94 21.895 29.515 27.325 21.265 95-99 24.995 29.56 22.759999999999998 22.685 100-104 21.315 31.19 24.465 23.03 105-109 21.98 28.255000000000003 26.83 22.935 110-114 24.725 27.855 24.66 22.759999999999998 115-119 19.505 30.764999999999997 26.46 23.27 120-124 18.884999999999998 29.965000000000003 25.77 25.380000000000003 125-129 19.85 33.09 22.215 24.845 130-134 23.115 30.895 25.040000000000003 20.95 135-139 23.86 29.915000000000003 22.61 23.615 140-144 24.921246062303116 28.206410320516024 26.3713185659283 20.501025051252565 145-149 22.61 30.855 25.53 21.005 150 22.25 29.125 27.450000000000003 21.175 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 0.0 22 0.5 23 1.0 24 4.0 25 5.5 26 6.0 27 8.0 28 10.0 29 12.5 30 12.0 31 17.0 32 28.0 33 32.5 34 44.0 35 48.5 36 76.5 37 157.0 38 310.5 39 330.5 40 263.0 41 301.0 42 283.5 43 255.0 44 220.0 45 201.0 46 217.0 47 179.0 48 125.5 49 94.0 50 61.0 51 51.5 52 54.5 53 36.5 54 29.5 55 30.0 56 23.5 57 17.5 58 17.5 59 23.5 60 27.0 61 19.5 62 16.0 63 21.5 64 53.5 65 66.5 66 36.0 67 19.5 68 21.0 69 23.0 70 19.0 71 14.5 72 11.0 73 7.5 74 8.0 75 10.5 76 13.5 77 9.0 78 5.0 79 4.0 80 2.5 81 2.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.005 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.574999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 75.61853351327036 42.025 2 15.294646873594242 17.0 3 4.048582995951417 6.75 4 1.0796221322537112 2.4 5 0.7197480881691408 2.0 6 0.4048582995951417 1.35 7 0.7647323436797121 2.9749999999999996 8 0.22492127755285649 1.0 9 0.3598740440845704 1.7999999999999998 >10 1.3945119208277104 16.35 >50 0.0449842555105713 1.4749999999999999 >100 0.0449842555105713 4.875 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 195 4.875 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 59 1.4749999999999999 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 50 1.25 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 49 1.225 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 39 0.975 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 35 0.8750000000000001 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 30 0.75 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 30 0.75 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 29 0.7250000000000001 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 27 0.675 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 23 0.575 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 22 0.5499999999999999 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 22 0.5499999999999999 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 22 0.5499999999999999 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 20 0.5 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 20 0.5 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 19 0.475 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 19 0.475 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 18 0.44999999999999996 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 18 0.44999999999999996 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 18 0.44999999999999996 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 17 0.42500000000000004 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 16 0.4 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 13 0.325 No Hit ATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATA 13 0.325 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 12 0.3 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 11 0.27499999999999997 No Hit ACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 11 0.27499999999999997 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 11 0.27499999999999997 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 10 0.25 No Hit TCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGT 10 0.25 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 10 0.25 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 10 0.25 No Hit ACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCAT 9 0.22499999999999998 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 9 0.22499999999999998 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 9 0.22499999999999998 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 9 0.22499999999999998 No Hit CTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAG 9 0.22499999999999998 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 9 0.22499999999999998 No Hit TGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCT 9 0.22499999999999998 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 9 0.22499999999999998 No Hit TGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATT 8 0.2 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 8 0.2 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 8 0.2 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 8 0.2 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 8 0.2 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 7 0.17500000000000002 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 7 0.17500000000000002 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 7 0.17500000000000002 No Hit GCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCA 7 0.17500000000000002 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 7 0.17500000000000002 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 7 0.17500000000000002 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 7 0.17500000000000002 No Hit CCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAA 7 0.17500000000000002 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 7 0.17500000000000002 No Hit GGCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGAT 7 0.17500000000000002 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 7 0.17500000000000002 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 7 0.17500000000000002 No Hit CATGTTGATGATTCATACAACTAAGTATATTTATCCCATTCTTCCTTACA 7 0.17500000000000002 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 7 0.17500000000000002 No Hit GTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCC 7 0.17500000000000002 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 7 0.17500000000000002 No Hit TAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGA 7 0.17500000000000002 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 6 0.15 No Hit GGTCGAACTACCAGAATGTCTAGAAATGTAGAGCTTAAACTAGAAAGGCT 6 0.15 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 6 0.15 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 6 0.15 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 6 0.15 No Hit GCGGTTAAGAAATTACAACCTTCCAAATAGGAACTAGCCAATCCATGGGT 6 0.15 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 6 0.15 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 6 0.15 No Hit GGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGG 6 0.15 No Hit TCTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTA 5 0.125 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 5 0.125 No Hit GAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAG 5 0.125 No Hit GCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCC 5 0.125 No Hit GGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 5 0.125 No Hit AGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCAT 5 0.125 No Hit CCCACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACA 5 0.125 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 5 0.125 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 5 0.125 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 5 0.125 No Hit CAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGA 5 0.125 No Hit GAGCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTA 5 0.125 No Hit GGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 5 0.125 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 5 0.125 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 5 0.125 No Hit GGGGAATTCGTAGATCCTCCAGACGTAGAGCACGTAGGGCTTTGAAACCA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0125 0.0 0.0 0.0 0.0 100-101 0.025 0.0 0.0 0.0 0.0 102-103 0.025 0.0 0.0 0.0 0.0 104-105 0.025 0.0 0.0 0.0 0.0 106-107 0.025 0.0 0.0 0.0 0.0 108-109 0.025 0.0 0.0 0.0 0.0 110-111 0.025 0.0 0.0 0.0 0.0 112-113 0.025 0.0 0.0 0.0 0.0 114-115 0.025 0.0 0.0 0.0 0.0 116-117 0.025 0.0 0.0 0.0 0.0 118-119 0.025 0.0 0.0 0.0 0.0 120-121 0.025 0.0 0.0 0.0 0.0 122-123 0.025 0.0 0.0 0.0 0.0 124-125 0.025 0.0 0.0 0.0 0.0 126-127 0.025 0.0 0.0 0.0 0.0 128-129 0.025 0.0 0.0 0.0 0.0 130-131 0.05 0.0 0.0 0.0 0.0 132-133 0.05 0.0 0.0 0.0 0.0 134-135 0.05 0.0 0.0 0.0 0.0 136-137 0.05 0.0 0.0 0.0 0.0 138 0.075 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCAGA 30 0.0018473949 72.0 8 AGCAGAT 30 0.0018473949 72.0 9 CATTAGC 30 0.0018473949 72.0 5 CCTCATT 30 0.0018473949 72.0 2 TCATTAG 30 0.0018473949 72.0 4 CCCTCAT 30 0.0018473949 72.0 1 TTAGCAG 30 0.0018473949 72.0 7 CTCATTA 35 0.0034045284 61.714283 3 ATATCAG 50 2.2297208E-6 23.039999 90-94 CGCGACC 45 2.6244928E-5 22.4 110-114 AACGCGA 45 2.6244928E-5 22.4 110-114 ATTGGTT 45 2.6244928E-5 22.4 135-139 GTATTAA 45 2.6244928E-5 22.4 100-104 AGTATTA 45 2.6244928E-5 22.4 100-104 ATCAGCC 45 2.6244928E-5 22.4 90-94 CCCAAGT 45 2.6244928E-5 22.4 95-99 TATCAGC 45 2.6244928E-5 22.4 90-94 TTGGTTG 45 2.6244928E-5 22.4 135-139 TAATAAC 45 2.6244928E-5 22.4 105-109 TTGAAAT 45 2.6244928E-5 22.4 140-144 >>END_MODULE ERR11006579 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006579_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.74875 37.0 37.0 37.0 37.0 37.0 2 36.2885 37.0 37.0 37.0 37.0 37.0 3 36.344 37.0 37.0 37.0 37.0 37.0 4 36.313 37.0 37.0 37.0 37.0 37.0 5 36.3215 37.0 37.0 37.0 37.0 37.0 6 36.4495 37.0 37.0 37.0 37.0 37.0 7 36.372 37.0 37.0 37.0 37.0 37.0 8 36.2695 37.0 37.0 37.0 37.0 37.0 9 36.416 37.0 37.0 37.0 37.0 37.0 10-14 36.375499999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.3992 37.0 37.0 37.0 37.0 37.0 20-24 36.3696 37.0 37.0 37.0 37.0 37.0 25-29 36.3558 37.0 37.0 37.0 37.0 37.0 30-34 36.29379999999999 37.0 37.0 37.0 37.0 37.0 35-39 36.3067 37.0 37.0 37.0 37.0 37.0 40-44 36.266 37.0 37.0 37.0 37.0 37.0 45-49 36.300799999999995 37.0 37.0 37.0 37.0 37.0 50-54 36.247 37.0 37.0 37.0 37.0 37.0 55-59 36.1705 37.0 37.0 37.0 37.0 37.0 60-64 36.1539 37.0 37.0 37.0 37.0 37.0 65-69 36.0754 37.0 37.0 37.0 37.0 37.0 70-74 36.0192 37.0 37.0 37.0 37.0 37.0 75-79 36.1259 37.0 37.0 37.0 37.0 37.0 80-84 36.05749999999999 37.0 37.0 37.0 37.0 37.0 85-89 36.0507 37.0 37.0 37.0 37.0 37.0 90-94 35.9959 37.0 37.0 37.0 37.0 37.0 95-99 35.981 37.0 37.0 37.0 37.0 37.0 100-104 35.9891 37.0 37.0 37.0 37.0 37.0 105-109 35.9153 37.0 37.0 37.0 37.0 37.0 110-114 35.869 37.0 37.0 37.0 37.0 37.0 115-119 35.8202 37.0 37.0 37.0 37.0 37.0 120-124 35.8293 37.0 37.0 37.0 37.0 37.0 125-129 35.842400000000005 37.0 37.0 37.0 37.0 37.0 130-134 35.656400000000005 37.0 37.0 37.0 37.0 37.0 135-139 35.8236 37.0 37.0 37.0 37.0 37.0 140-144 35.650999999999996 37.0 37.0 37.0 37.0 37.0 145-149 35.5279 37.0 37.0 37.0 37.0 37.0 150 35.5985 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 1.0 20 0.0 21 1.0 22 0.0 23 1.0 24 1.0 25 9.0 26 6.0 27 18.0 28 16.0 29 14.0 30 23.0 31 42.0 32 46.0 33 70.0 34 143.0 35 488.0 36 2938.0 37 182.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.285641934667005 26.589009875917952 19.01747277791846 26.107875411496583 2 25.424999999999997 25.5 34.525 14.549999999999999 3 20.200000000000003 26.75 33.525 19.525000000000002 4 21.95 30.2 27.700000000000003 20.150000000000002 5 23.05 29.45 29.9 17.599999999999998 6 19.525000000000002 35.0 27.55 17.925 7 20.275000000000002 19.525000000000002 42.275 17.925 8 19.325 26.174999999999997 32.725 21.775 9 22.55 20.825 36.15 20.474999999999998 10-14 23.78 26.415 29.64 20.165 15-19 23.36 25.415 31.225 20.0 20-24 24.755 24.349999999999998 30.020000000000003 20.875 25-29 24.18 24.21 30.73 20.880000000000003 30-34 24.57 24.36 31.11 19.96 35-39 24.154999999999998 24.169999999999998 30.264999999999997 21.41 40-44 22.24 26.200000000000003 29.685 21.875 45-49 22.475 27.665 29.085 20.775 50-54 22.06 27.1 28.92 21.92 55-59 23.04 25.180000000000003 29.21 22.57 60-64 22.33 24.675 30.099999999999998 22.895 65-69 23.01 25.06 30.455 21.475 70-74 23.705000000000002 25.81 29.459999999999997 21.025 75-79 24.11 24.4 30.7 20.79 80-84 24.145 25.745 29.5 20.61 85-89 24.72 25.61 27.66 22.009999999999998 90-94 23.18 25.064999999999998 29.89 21.865000000000002 95-99 23.215 24.42 29.82 22.545 100-104 24.415 22.830000000000002 31.180000000000003 21.575 105-109 25.624999999999996 23.169999999999998 29.09 22.115000000000002 110-114 25.074999999999996 23.265 29.654999999999998 22.005 115-119 23.69 24.685000000000002 29.830000000000002 21.795 120-124 24.535 24.765 28.68 22.02 125-129 23.36 24.84 30.56 21.240000000000002 130-134 23.095 26.605 28.325 21.975 135-139 23.46 25.405 30.39 20.745 140-144 23.035 24.47 30.959999999999997 21.535 145-149 22.975 24.19 31.255 21.58 150 23.275000000000002 22.925 30.625000000000004 23.175 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.0 22 1.5 23 1.0 24 3.0 25 6.5 26 11.0 27 17.5 28 23.5 29 35.5 30 38.0 31 33.5 32 34.5 33 40.5 34 61.0 35 91.5 36 144.0 37 238.5 38 269.5 39 222.0 40 234.0 41 271.5 42 234.5 43 220.5 44 219.5 45 184.0 46 158.0 47 126.5 48 111.0 49 92.0 50 61.5 51 42.0 52 38.0 53 35.5 54 32.0 55 31.5 56 31.0 57 33.5 58 29.0 59 25.5 60 31.0 61 31.0 62 35.5 63 52.0 64 60.5 65 55.5 66 43.0 67 29.0 68 24.0 69 20.5 70 24.5 71 25.5 72 17.5 73 15.0 74 13.5 75 7.5 76 3.5 77 5.0 78 4.0 79 2.5 80 4.5 81 3.5 82 1.0 83 1.0 84 1.0 85 0.5 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.275 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.150000000000006 #Duplication Level Percentage of deduplicated Percentage of total 1 74.5374094931617 46.325 2 15.68785197103781 19.5 3 4.3845534995977475 8.175 4 1.7699115044247788 4.3999999999999995 5 1.3676588897827837 4.25 6 0.4424778761061947 1.6500000000000001 7 0.32180209171359614 1.4000000000000001 8 0.36202735317779566 1.7999999999999998 9 0.12067578439259855 0.675 >10 0.9654062751407884 10.05 >50 0.04022526146419952 1.775 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 71 1.775 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 33 0.8250000000000001 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 27 0.675 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 27 0.675 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 25 0.625 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 21 0.525 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 20 0.5 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 19 0.475 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 17 0.42500000000000004 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 17 0.42500000000000004 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 15 0.375 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 15 0.375 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 15 0.375 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 14 0.35000000000000003 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 14 0.35000000000000003 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 13 0.325 No Hit AATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTAT 13 0.325 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 13 0.325 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 13 0.325 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 13 0.325 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 12 0.3 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 12 0.3 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 12 0.3 No Hit TACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGG 12 0.3 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 10 0.25 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 9 0.22499999999999998 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 9 0.22499999999999998 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 9 0.22499999999999998 No Hit AAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATC 8 0.2 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 8 0.2 No Hit ATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCT 8 0.2 No Hit GACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCC 8 0.2 No Hit GTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACT 8 0.2 No Hit TTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGG 8 0.2 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 8 0.2 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 8 0.2 No Hit TCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTG 8 0.2 No Hit GTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTATGCC 7 0.17500000000000002 No Hit TTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGA 7 0.17500000000000002 No Hit ATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATAT 7 0.17500000000000002 No Hit CTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATT 7 0.17500000000000002 No Hit AAGATGGTTCAGCTAATTAACAACATATATAGAATATAGTTAGAAGAGAC 7 0.17500000000000002 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 7 0.17500000000000002 No Hit TGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTC 7 0.17500000000000002 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 7 0.17500000000000002 No Hit TATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACA 6 0.15 No Hit AAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAG 6 0.15 No Hit ATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCAC 6 0.15 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 6 0.15 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 6 0.15 No Hit TATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACC 6 0.15 No Hit CTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTT 6 0.15 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 6 0.15 No Hit GAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCG 6 0.15 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 6 0.15 No Hit ATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAA 6 0.15 No Hit GAAGCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCT 5 0.125 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 5 0.125 No Hit GAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGAC 5 0.125 No Hit TGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGA 5 0.125 No Hit AGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTTAT 5 0.125 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 5 0.125 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 5 0.125 No Hit GATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTC 5 0.125 No Hit GGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGGCCTGGTCT 5 0.125 No Hit GAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGC 5 0.125 No Hit GGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCG 5 0.125 No Hit CTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAG 5 0.125 No Hit GTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCAT 5 0.125 No Hit CTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTT 5 0.125 No Hit ATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGA 5 0.125 No Hit TGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCT 5 0.125 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 5 0.125 No Hit AGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCT 5 0.125 No Hit CTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGG 5 0.125 No Hit ATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTT 5 0.125 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 5 0.125 No Hit TAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAG 5 0.125 No Hit GAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATT 5 0.125 No Hit GTTTCTGGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTAT 5 0.125 No Hit TTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGA 5 0.125 No Hit CTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCC 5 0.125 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 5 0.125 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 5 0.125 No Hit GAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAAT 5 0.125 No Hit GGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACA 5 0.125 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 5 0.125 No Hit GTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGTGGGA 5 0.125 No Hit ATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTG 5 0.125 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0125 0.0 0.0 0.0 0.0 100-101 0.025 0.0 0.0 0.0 0.0 102-103 0.025 0.0 0.0 0.0 0.0 104-105 0.025 0.0 0.0 0.0 0.0 106-107 0.025 0.0 0.0 0.0 0.0 108-109 0.025 0.0 0.0 0.0 0.0 110-111 0.025 0.0 0.0 0.0 0.0 112-113 0.025 0.0 0.0 0.0 0.0 114-115 0.025 0.0 0.0 0.0 0.0 116-117 0.025 0.0 0.0 0.0 0.0 118-119 0.025 0.0 0.0 0.0 0.0 120-121 0.025 0.0 0.0 0.0 0.0 122-123 0.025 0.0 0.0 0.0 0.0 124-125 0.025 0.0 0.0 0.0 0.0 126-127 0.025 0.0 0.0 0.0 0.0 128-129 0.025 0.0 0.0 0.0 0.0 130-131 0.05 0.0 0.0 0.0 0.0 132-133 0.05 0.0 0.0 0.0 0.0 134-135 0.05 0.0 0.0 0.0 0.0 136-137 0.05 0.0 0.0 0.0 0.0 138 0.075 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGGAGT 10 0.0067147487 145.81013 1 GAGTCGG 10 0.0069754543 143.9875 4 CGGGCCA 10 0.0069754543 143.9875 8 AGTCGGG 10 0.0069754543 143.9875 5 TATTTCT 10 0.0069754543 143.9875 3 ATTTCTA 10 0.0069754543 143.9875 4 TCGGGCC 10 0.0069754543 143.9875 7 GGGCCAG 10 0.0069754543 143.9875 9 >>END_MODULE Read 2696172 spots for ERR11006579.sra Written 2696172 spots for ERR11006579.sra Read 2696172 spots for ERR11006579.sra Written 2696172 spots for ERR11006579.sra Read 2696172 spots for ERR11006579.sra Written 2696172 spots for ERR11006579.sra Read 2696172 spots for ERR11006579.sra Written 2696172 spots for ERR11006579.sra Read 2696172 spots for ERR11006579.sra Written 2696172 spots for ERR11006579.sra Read 2696172 spots for ERR11006579.sra Written 2696172 spots for ERR11006579.sra Read 2696172 spots for ERR11006579.sra Written 2696172 spots for ERR11006579.sra Read 2696172 spots for ERR11006579.sra Written 2696172 spots for ERR11006579.sra Read 2696172 spots for ERR11006579.sra Written 2696172 spots for ERR11006579.sra Read 2696172 spots for ERR11006579.sra Written 2696172 spots for ERR11006579.sra Read 2696172 spots for ERR11006579.sra Written 2696172 spots for ERR11006579.sra Read 2696172 spots for ERR11006579.sra Written 2696172 spots for ERR11006579.sra Read 2696172 spots for ERR11006579.sra Written 2696172 spots for ERR11006579.sra Read 2696178 spots for ERR11006579.sra Written 2696178 spots for ERR11006579.sra Read 2696172 spots for ERR11006579.sra Written 2696172 spots for ERR11006579.sra Read 2696172 spots for ERR11006579.sra Written 2696172 spots for ERR11006579.sra Read 2696172 spots for ERR11006579.sra Written 2696172 spots for ERR11006579.sra Read 2696172 spots for ERR11006579.sra Written 2696172 spots for ERR11006579.sra Read 2696172 spots for ERR11006579.sra Written 2696172 spots for ERR11006579.sra Read 2696172 spots for ERR11006579.sra Written 2696172 spots for ERR11006579.sra SRR ids: ['ERR11006579.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_nc5e3r4e ERR11006579.sra spots: 53923446 blocks: [[1, 2696172], [2696173, 5392344], [5392345, 8088516], [8088517, 10784688], [10784689, 13480860], [13480861, 16177032], [16177033, 18873204], [18873205, 21569376], [21569377, 24265548], [24265549, 26961720], [26961721, 29657892], [29657893, 32354064], [32354065, 35050236], [35050237, 37746408], [37746409, 40442580], [40442581, 43138752], [43138753, 45834924], [45834925, 48531096], [48531097, 51227268], [51227269, 53923446]] ERR11006579 file size 19814110 ERR11006579 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006579 ERR11006579_1.fastq ERR11006579_2.fastq Input file: ERR11006579_1.fastq Paired file: ERR11006579_2.fastq trimmed: ERR11006579-trimmed-pair1.fastq, ERR11006579-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 17:53:54 2024 >> started Fri Dec 6 17:55:16 2024 >> done (81.617s) 53923446 read pairs processed; of these: 144 ( 0.00%) short read pairs filtered out after trimming by size control 451 ( 0.00%) empty read pairs filtered out after trimming by size control 53922851 (100.00%) read pairs available; of these: 97000 ( 0.18%) trimmed read pairs available after processing 53825851 (99.82%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 3 0.00% 20 9 0.00% 21 18 0.00% 22 25 0.00% 23 8 0.00% 24 13 0.00% 25 12 0.00% 26 15 0.00% 27 17 0.00% 28 19 0.00% 29 13 0.00% 30 19 0.00% 31 41 0.00% 32 24 0.00% 33 20 0.00% 34 23 0.00% 35 28 0.00% 36 37 0.00% 37 27 0.00% 38 23 0.00% 39 25 0.00% 40 24 0.00% 41 24 0.00% 42 30 0.00% 43 28 0.00% 44 37 0.00% 45 34 0.00% 46 37 0.00% 47 38 0.00% 48 40 0.00% 49 44 0.00% 50 37 0.00% 51 39 0.00% 52 39 0.00% 53 69 0.00% 54 43 0.00% 55 61 0.00% 56 60 0.00% 57 58 0.00% 58 56 0.00% 59 57 0.00% 60 57 0.00% 61 50 0.00% 62 84 0.00% 63 73 0.00% 64 90 0.00% 65 81 0.00% 66 75 0.00% 67 98 0.00% 68 98 0.00% 69 84 0.00% 70 95 0.00% 71 92 0.00% 72 118 0.00% 73 116 0.00% 74 117 0.00% 75 132 0.00% 76 133 0.00% 77 146 0.00% 78 157 0.00% 79 180 0.00% 80 161 0.00% 81 158 0.00% 82 177 0.00% 83 214 0.00% 84 234 0.00% 85 190 0.00% 86 260 0.00% 87 283 0.00% 88 268 0.00% 89 254 0.00% 90 310 0.00% 91 329 0.00% 92 320 0.00% 93 313 0.00% 94 343 0.00% 95 378 0.00% 96 379 0.00% 97 409 0.00% 98 402 0.00% 99 449 0.00% 100 485 0.00% 101 411 0.00% 102 532 0.00% 103 540 0.00% 104 589 0.00% 105 605 0.00% 106 607 0.00% 107 669 0.00% 108 622 0.00% 109 702 0.00% 110 759 0.00% 111 865 0.00% 112 921 0.00% 113 852 0.00% 114 932 0.00% 115 951 0.00% 116 1148 0.00% 117 1089 0.00% 118 1115 0.00% 119 1116 0.00% 120 1335 0.00% 121 1337 0.00% 122 1525 0.00% 123 1474 0.00% 124 1563 0.00% 125 1747 0.00% 126 1535 0.00% 127 1456 0.00% 128 1580 0.00% 129 1722 0.00% 130 1908 0.00% 131 1970 0.00% 132 2141 0.00% 133 1994 0.00% 134 2095 0.00% 135 2063 0.00% 136 2151 0.00% 137 2220 0.00% 138 2296 0.00% 139 2479 0.00% 140 2529 0.00% 141 2775 0.01% 142 3015 0.01% 143 3220 0.01% 144 3196 0.01% 145 3427 0.01% 146 4228 0.01% 147 4003 0.01% 148 4125 0.01% 149 5197 0.01% 150 53825851 99.82% 53922851 reads passed initial QC criterion=sequence-density sequence-density=0.38 sequence-density-rank=1 fanout-score=0.00 fanout-score-rank=34 prefix-density=0.00 prefix-fanout=1.0 sequence=TGCGGGAACTTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=33 fanout-score=51.73 fanout-score-rank=1 prefix-density=0.24 prefix-fanout=2.0 sequence=AAAAACAGTAGAAGTAGAACAGGTATAAATAAGAAAATCTTAGTTAAGAGGGTTCATGTAAAGAACAGGTTCTAAATCACGATCGATTCCCTTTTCAAAACCTGCTGCAGCAGCTCGGGCTCTTCCTGCATGCCACAAATGGCCCACAAAAAAGAAGAATCCTAGAACAAAATGAGAAGTCGATAACCAACTTCTAGGAGAGACATAATTAACTGCATTGATCTCGGTAGCTACGCCACCCACGGAATTTAAAGAGCCTAAAGGAGCATGGGTCATATATTCCGCTGAACGTCGTTCTTGCCAAGGTTGTATGTCTTTTTTCAACCTACTCAAGTCCAAACCGTTGGGCCCCCTTAGAGGTTCTAACCATGGAGCACGGAGGTCCCAAAAACGCATAGTTTCCCCTCCAAAGATAACCTCTCCCGTTGGGGAACGCATTAGATATTTACCTAAACCTGTGGGTCCTTGAGCAGATCCCACATTAGCTCCAAGACGCTGGTCTCTAACTAGA criterion=sequence-density sequence-density=0.18 sequence-density-rank=1 fanout-score=3.62 fanout-score-rank=26 prefix-density=0.31 prefix-fanout=2.2 sequence=CAACTGCGGGTACATGTGAAGAAATGATGAAGAGAGCTGTTTTTGCGAGAGAATTAGGTGTTCCTATTGTAATGCATGACTACTTAACTGGGGGATTCACCGCAAATACTACTTTGGCTCATTATTGCCGCGACAATGGCTTACTTCTTCACATTCACCGTGCAATGCATGCAGTTATTGATAGACAGAAAAATCATGGTATGCATTTCCGTGTATTAGCTAAAGCATTGCGTATGTCTGGGGGAGATCATATCCACGCCGGTACAGTAGTAGGTAAGTTAGAAGGGGAACGCGAAATCACTTTAGGTTTTGTTGATTTATTGCGCGACGATTTTATTGAAAAAGATCGTGCTCGCGGTATCTTTTTCACTCAGGACTGGGTATCCATGCCAGGTGTTATACCAGTAGCTTCAGGTGGTATTCATGTTTGGCATATGCCAGCTCTGACCGAAATCTTTGGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=27 fanout-score=39.03 fanout-score-rank=1 prefix-density=0.29 prefix-fanout=1.9 sequence=TGGCGTAGCAGGCCTCCCTTTGGGAGGCCCGCGCGACGGGCTATTAGCTCAGTGGTAGAGCGCGCCCCTGATAATTGCGTCGTTGTGCCTGGGCTGTGAGGGCTCTCAGCCACATGGATAGTTCAATGTGCTCATCAGCGCCTGACCCGAAGATGTGGATCATCCAAGGCACATTAGCATGGCGTACTCCTCCTGTTTGAATCGGAGTTTGAAACCAAACAAACTTCTCCTCAGGAGGATAGATGGGGCGATTCAGGTGAGATCCCATGTAGATCTAACTTTCTATTCACTCGTGGGATCCGGGCGGTCCGGGGGGGGCCACCACAGCTCCTCTCTTCTCGAGAATCCATACACCCCTTATCAGTGTATGGAGAGCTATCTCTCGACCACAGGTTGAGGTCCGTCCTCAATGGGAAAATGGAGCACCTAACAACGCATCTTCACAGACCAAGAACTACGAGA ERR11006579 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 17:56:32 Started mapping on | Dec 06 17:56:36 Finished on | Dec 06 18:01:54 Mapping speed, Million of reads per hour | 610.45 Number of input reads | 53922851 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 42159154 Uniquely mapped reads % | 78.18% Average mapped length | 298.70 Number of splices: Total | 12949674 Number of splices: Annotated (sjdb) | 11989995 Number of splices: GT/AG | 12606405 Number of splices: GC/AG | 142228 Number of splices: AT/AC | 21307 Number of splices: Non-canonical | 179734 Mismatch rate per base, % | 0.30% Deletion rate per base | 0.01% Deletion average length | 1.76 Insertion rate per base | 0.01% Insertion average length | 2.00 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 10195436 % of reads mapped to multiple loci | 18.91% Number of reads mapped to too many loci | 6425 % of reads mapped to too many loci | 0.01% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.74% % of reads unmapped: other | 0.15% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1568261 1568261 1568261 N_multimapping 10195436 10195436 10195436 N_noFeature 10735272 38787205 13026648 N_ambiguous 1806853 61303 725594 UnstrandedReadsAssigned:29617029 PositiveStrandReadsAssigned:3310646 NegativeStrandReadsAssigned:28406912 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006579 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006579-trimmed-pair1.fastq ERR11006579-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 53,922,851 reads, 31,225,000 reads pseudoaligned [quant] estimated average fragment length: 319.363 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,144 rounds 52973 ERR11006579.ke.tsv 35125 ERR11006579.se.tsv 88098 total ==> ERR11006579.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 618.096 0 0 PNS24247 1044 725.637 20.6007 0.731903 PNS24249 1928 1609.64 117.919 1.88862 PNS24246 1044 725.637 20.6007 0.731903 PNS24248 1044 725.637 20.6007 0.731903 PNS24244 1471 1152.64 30.279 0.677235 PNS24243 293 38.8918 0 0 KQK14069 1603 1284.64 2683.3 53.8493 KQK14071 474 161.795 12.1475 1.93558 ==> ERR11006579.se.tsv <== BRADI_1g14170v3 2971 BRADI_1g53295v3 672 BRADI_1g59795v3 319 BRADI_1g07683v3 0 BRADI_1g00485v3 2 BRADI_1g20270v3 131 BRADI_1g74790v3 246 BRADI_1g09890v3 0 BRADI_1g77505v3 110 BRADI_1g48960v3 3 ERR11006579 completed mapping pipeline successfully