Starting /dee2/code/volunteer_pipeline.sh ERR11006580 current disk space = 1550684094464 free memory = 1602988040 ERR11006580 SRAfilesize 0f9f7cd894df6deb60b98bf424483f47 ERR11006580.sra ERR11006580.sra file validated ERR11006580 is paired end ERR11006580 is conventional basespace ERR11006580 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006580_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.315 37.0 37.0 37.0 37.0 37.0 2 36.215 37.0 37.0 37.0 37.0 37.0 3 36.2745 37.0 37.0 37.0 37.0 37.0 4 36.353 37.0 37.0 37.0 37.0 37.0 5 36.3935 37.0 37.0 37.0 37.0 37.0 6 36.426 37.0 37.0 37.0 37.0 37.0 7 36.2075 37.0 37.0 37.0 37.0 37.0 8 36.39 37.0 37.0 37.0 37.0 37.0 9 36.4135 37.0 37.0 37.0 37.0 37.0 10-14 36.398 37.0 37.0 37.0 37.0 37.0 15-19 36.422399999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.349000000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.254599999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.260999999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.1997 37.0 37.0 37.0 37.0 37.0 40-44 36.193799999999996 37.0 37.0 37.0 37.0 37.0 45-49 36.180400000000006 37.0 37.0 37.0 37.0 37.0 50-54 36.1458 37.0 37.0 37.0 37.0 37.0 55-59 36.119400000000006 37.0 37.0 37.0 37.0 37.0 60-64 36.0911 37.0 37.0 37.0 37.0 37.0 65-69 36.10109999999999 37.0 37.0 37.0 37.0 37.0 70-74 36.0064 37.0 37.0 37.0 37.0 37.0 75-79 36.0587 37.0 37.0 37.0 37.0 37.0 80-84 36.006600000000006 37.0 37.0 37.0 37.0 37.0 85-89 35.9539 37.0 37.0 37.0 37.0 37.0 90-94 35.827799999999996 37.0 37.0 37.0 37.0 37.0 95-99 35.8711 37.0 37.0 37.0 37.0 37.0 100-104 35.873000000000005 37.0 37.0 37.0 37.0 37.0 105-109 35.800799999999995 37.0 37.0 37.0 37.0 37.0 110-114 35.7377 37.0 37.0 37.0 37.0 37.0 115-119 35.706399999999995 37.0 37.0 37.0 37.0 37.0 120-124 35.7236 37.0 37.0 37.0 37.0 37.0 125-129 35.62089999999999 37.0 37.0 37.0 37.0 37.0 130-134 35.476099999999995 37.0 37.0 37.0 37.0 37.0 135-139 35.4695 37.0 37.0 37.0 37.0 37.0 140-144 35.141 37.0 37.0 37.0 29.8 37.0 145-149 35.357 37.0 37.0 37.0 34.6 37.0 150 35.411 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 2.0 22 0.0 23 4.0 24 1.0 25 6.0 26 14.0 27 13.0 28 18.0 29 50.0 30 57.0 31 51.0 32 96.0 33 107.0 34 142.0 35 316.0 36 2801.0 37 321.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.35 13.200000000000001 15.725 33.725 2 28.849999999999998 11.3 25.85 34.0 3 22.85 12.45 26.6 38.1 4 27.775 15.825 25.424999999999997 30.975 5 28.1 20.25 25.474999999999998 26.174999999999997 6 25.8 28.599999999999998 20.225 25.374999999999996 7 18.099999999999998 31.15 33.074999999999996 17.675 8 16.775000000000002 30.2 32.475 20.549999999999997 9 17.925 28.849999999999998 33.575 19.650000000000002 10-14 19.99 31.71 27.034999999999997 21.265 15-19 20.595 33.085 24.64 21.68 20-24 19.895 28.860000000000003 27.794999999999998 23.45 25-29 21.445 31.34 25.874999999999996 21.34 30-34 24.08 30.209999999999997 24.505 21.205 35-39 22.515 30.695 25.180000000000003 21.61 40-44 20.595 30.409999999999997 25.335 23.66 45-49 20.865000000000002 29.205 27.139999999999997 22.79 50-54 21.39 32.265 25.174999999999997 21.17 55-59 21.67 29.945 23.745 24.64 60-64 20.630000000000003 31.545 24.935 22.89 65-69 21.58 29.955 25.045 23.419999999999998 70-74 23.28 29.975 22.0 24.745 75-79 23.244999999999997 29.475 24.48 22.8 80-84 22.470000000000002 30.240000000000002 24.005000000000003 23.285 85-89 23.055 29.04 24.815 23.09 90-94 21.97 29.865000000000002 26.055 22.11 95-99 24.15 28.71 23.24 23.9 100-104 21.94 30.409999999999997 23.87 23.78 105-109 22.470000000000002 29.060000000000002 24.975 23.494999999999997 110-114 23.48 27.99 24.64 23.89 115-119 20.395 30.59 25.41 23.605 120-124 20.71 29.5 24.93 24.86 125-129 21.015 30.915 23.1 24.97 130-134 22.689999999999998 30.605 24.595 22.11 135-139 23.44 30.005 22.98 23.575 140-144 23.875 29.195 25.735000000000003 21.195 145-149 22.525000000000002 29.775000000000002 24.445 23.255 150 24.025 26.55 26.474999999999998 22.95 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.0 22 3.0 23 3.5 24 3.0 25 3.0 26 6.0 27 9.0 28 18.5 29 20.5 30 12.5 31 18.0 32 22.0 33 31.5 34 48.5 35 55.0 36 83.5 37 155.5 38 262.0 39 288.5 40 261.5 41 275.0 42 262.0 43 219.5 44 187.5 45 209.0 46 212.0 47 170.5 48 134.0 49 103.0 50 84.5 51 60.5 52 50.5 53 42.5 54 34.0 55 38.0 56 28.0 57 27.5 58 29.5 59 31.0 60 31.5 61 22.5 62 22.5 63 31.5 64 53.5 65 71.5 66 49.5 67 23.0 68 22.5 69 20.5 70 18.0 71 20.0 72 21.0 73 16.0 74 14.0 75 13.5 76 8.5 77 8.0 78 12.0 79 9.5 80 3.0 81 1.0 82 0.5 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.9 #Duplication Level Percentage of deduplicated Percentage of total 1 81.14567526555388 53.474999999999994 2 11.191198786039454 14.75 3 3.110773899848255 6.15 4 1.7450682852807284 4.6 5 0.6069802731411229 2.0 6 0.37936267071320184 1.5 7 0.4552352048558422 2.1 8 0.15174506828528073 0.8 9 0.2276176024279211 1.35 >10 0.9484066767830045 10.85 >50 0.03793626707132018 2.4250000000000003 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 97 2.4250000000000003 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 36 0.8999999999999999 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 35 0.8750000000000001 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 33 0.8250000000000001 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 29 0.7250000000000001 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 29 0.7250000000000001 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 26 0.65 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 20 0.5 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 16 0.4 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 16 0.4 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 15 0.375 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 14 0.35000000000000003 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 14 0.35000000000000003 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 13 0.325 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 13 0.325 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 12 0.3 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 12 0.3 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 12 0.3 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 12 0.3 No Hit TCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCG 11 0.27499999999999997 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 11 0.27499999999999997 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 11 0.27499999999999997 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 11 0.27499999999999997 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 11 0.27499999999999997 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 11 0.27499999999999997 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 11 0.27499999999999997 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 9 0.22499999999999998 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 9 0.22499999999999998 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 9 0.22499999999999998 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 9 0.22499999999999998 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 9 0.22499999999999998 No Hit CACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAAT 9 0.22499999999999998 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 8 0.2 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 8 0.2 No Hit GGCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGAT 8 0.2 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 8 0.2 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 7 0.17500000000000002 No Hit ACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCAT 7 0.17500000000000002 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 7 0.17500000000000002 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 7 0.17500000000000002 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 7 0.17500000000000002 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 7 0.17500000000000002 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 7 0.17500000000000002 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 7 0.17500000000000002 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 7 0.17500000000000002 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 7 0.17500000000000002 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 7 0.17500000000000002 No Hit AGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGG 7 0.17500000000000002 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 6 0.15 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 6 0.15 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 6 0.15 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 6 0.15 No Hit ACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAG 6 0.15 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 6 0.15 No Hit CACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCG 6 0.15 No Hit TCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTT 6 0.15 No Hit TAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGA 6 0.15 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 6 0.15 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 5 0.125 No Hit GGGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACA 5 0.125 No Hit CGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTT 5 0.125 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 5 0.125 No Hit TCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGT 5 0.125 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 5 0.125 No Hit AACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTA 5 0.125 No Hit CGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGA 5 0.125 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 5 0.125 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 5 0.125 No Hit GTCCAAACCGTTGGGCCCCCTTAGAGGTTCTAACCATGGAGCACGGAGGT 5 0.125 No Hit CAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTG 5 0.125 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 5 0.125 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 5 0.125 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 5 0.125 No Hit GCGGTTATGAGTACGACCGGGCGTGAACGGTACTCGGTCCTCCGGATTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.05 0.0 0.0 0.0 0.0 84-85 0.05 0.0 0.0 0.0 0.0 86-87 0.05 0.0 0.0 0.0 0.0 88-89 0.05 0.0 0.0 0.0 0.0 90-91 0.05 0.0 0.0 0.0 0.0 92-93 0.05 0.0 0.0 0.0 0.0 94-95 0.05 0.0 0.0 0.0 0.0 96-97 0.05 0.0 0.0 0.0 0.0 98-99 0.05 0.0 0.0 0.0 0.0 100-101 0.075 0.0 0.0 0.0 0.0 102-103 0.075 0.0 0.0 0.0 0.0 104-105 0.075 0.0 0.0 0.0 0.0 106-107 0.1 0.0 0.0 0.0 0.0 108-109 0.125 0.0 0.0 0.0 0.0 110-111 0.125 0.0 0.0 0.0 0.0 112-113 0.15 0.0 0.0 0.0 0.0 114-115 0.15 0.0 0.0 0.0 0.0 116-117 0.1875 0.0 0.0 0.0 0.0 118-119 0.2 0.0 0.0 0.0 0.0 120-121 0.2625 0.0 0.0 0.0 0.0 122-123 0.325 0.0 0.0 0.0 0.0 124-125 0.3625 0.0 0.0 0.0 0.0 126-127 0.375 0.0 0.0 0.0 0.0 128-129 0.4 0.0 0.0 0.0 0.0 130-131 0.475 0.0 0.0 0.0 0.0 132-133 0.5125 0.0 0.0 0.0 0.0 134-135 0.5625 0.0 0.0 0.0 0.0 136-137 0.5874999999999999 0.0 0.0 0.0 0.0 138 0.6 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTTCT 20 3.687869E-4 108.0 1 AACAAGC 25 8.956223E-4 86.399994 9 TATAACA 25 8.956223E-4 86.399994 6 CCCATAT 25 8.956223E-4 86.399994 2 ATAACAA 25 8.956223E-4 86.399994 7 TAACAAG 25 8.956223E-4 86.399994 8 CCATATA 25 8.956223E-4 86.399994 3 CATATAA 25 8.956223E-4 86.399994 4 ATATAAC 25 8.956223E-4 86.399994 5 CTTTCTT 35 0.0034045284 61.714283 2 TTCTTTT 40 0.005777437 54.0 4 TCTTTTC 40 0.005777437 54.0 5 CTTTTCT 45 0.009205684 48.0 6 TTTCTTC 45 0.009205684 48.0 8 TTTTCTT 45 0.009205684 48.0 7 CTTCAAA 40 0.007966741 36.0 6 TTCAAAA 40 3.1003024E-4 36.0 7 TCAAAAA 40 3.1003024E-4 36.0 8 AAATTCT 40 3.1003024E-4 21.599998 15-19 TTATATG 40 3.1003024E-4 21.599998 20-24 >>END_MODULE ERR11006580 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006580_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.54375 37.0 37.0 37.0 37.0 37.0 2 36.1135 37.0 37.0 37.0 37.0 37.0 3 36.237 37.0 37.0 37.0 37.0 37.0 4 36.2125 37.0 37.0 37.0 37.0 37.0 5 36.2695 37.0 37.0 37.0 37.0 37.0 6 36.1845 37.0 37.0 37.0 37.0 37.0 7 36.208 37.0 37.0 37.0 37.0 37.0 8 36.4185 37.0 37.0 37.0 37.0 37.0 9 36.2155 37.0 37.0 37.0 37.0 37.0 10-14 36.2339 37.0 37.0 37.0 37.0 37.0 15-19 36.2384 37.0 37.0 37.0 37.0 37.0 20-24 36.2265 37.0 37.0 37.0 37.0 37.0 25-29 36.15599999999999 37.0 37.0 37.0 37.0 37.0 30-34 36.1195 37.0 37.0 37.0 37.0 37.0 35-39 36.0992 37.0 37.0 37.0 37.0 37.0 40-44 36.0783 37.0 37.0 37.0 37.0 37.0 45-49 36.0317 37.0 37.0 37.0 37.0 37.0 50-54 35.986799999999995 37.0 37.0 37.0 37.0 37.0 55-59 36.0481 37.0 37.0 37.0 37.0 37.0 60-64 35.9508 37.0 37.0 37.0 37.0 37.0 65-69 35.8935 37.0 37.0 37.0 37.0 37.0 70-74 35.8356 37.0 37.0 37.0 37.0 37.0 75-79 35.863899999999994 37.0 37.0 37.0 37.0 37.0 80-84 35.80749999999999 37.0 37.0 37.0 37.0 37.0 85-89 35.7496 37.0 37.0 37.0 37.0 37.0 90-94 35.80989999999999 37.0 37.0 37.0 37.0 37.0 95-99 35.6042 37.0 37.0 37.0 37.0 37.0 100-104 35.563900000000004 37.0 37.0 37.0 37.0 37.0 105-109 35.5573 37.0 37.0 37.0 37.0 37.0 110-114 35.4901 37.0 37.0 37.0 37.0 37.0 115-119 35.389300000000006 37.0 37.0 37.0 34.6 37.0 120-124 35.264500000000005 37.0 37.0 37.0 29.8 37.0 125-129 35.27909999999999 37.0 37.0 37.0 32.2 37.0 130-134 35.203599999999994 37.0 37.0 37.0 29.8 37.0 135-139 35.072500000000005 37.0 37.0 37.0 25.0 37.0 140-144 35.01630000000001 37.0 37.0 37.0 27.4 37.0 145-149 35.0923 37.0 37.0 37.0 29.8 37.0 150 34.883 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 0.0 17 0.0 18 1.0 19 2.0 20 0.0 21 0.0 22 2.0 23 5.0 24 10.0 25 7.0 26 12.0 27 24.0 28 20.0 29 38.0 30 49.0 31 62.0 32 78.0 33 111.0 34 183.0 35 551.0 36 2683.0 37 161.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.17197452229299 25.299363057324843 19.821656050955415 25.70700636942675 2 27.275 24.325 30.925000000000004 17.474999999999998 3 21.975 27.450000000000003 32.35 18.224999999999998 4 24.9 27.6 28.299999999999997 19.2 5 25.124999999999996 30.5 26.900000000000002 17.474999999999998 6 22.3 31.5 27.825 18.375 7 20.4 22.400000000000002 38.550000000000004 18.65 8 24.55 21.025 30.625000000000004 23.799999999999997 9 23.9 19.2 33.425 23.474999999999998 10-14 24.169999999999998 26.295 28.325 21.21 15-19 24.015 24.91 30.240000000000002 20.835 20-24 24.695 24.46 29.604999999999997 21.240000000000002 25-29 24.44 24.67 29.445 21.445 30-34 24.925 23.745 30.520000000000003 20.810000000000002 35-39 24.709999999999997 24.515 29.625 21.15 40-44 23.52 25.174999999999997 29.69 21.615000000000002 45-49 23.655 25.7 29.54 21.105 50-54 23.155 25.545 29.17 22.13 55-59 23.375 25.39 28.544999999999998 22.689999999999998 60-64 23.13 24.67 29.659999999999997 22.54 65-69 23.565 25.455 28.88 22.1 70-74 24.245 24.815 29.515 21.425 75-79 23.775 24.895 29.74 21.59 80-84 24.85 25.15 28.910000000000004 21.09 85-89 25.275 24.895 27.705000000000002 22.125 90-94 23.064999999999998 24.745 28.84 23.35 95-99 24.485 23.835 29.685 21.995 100-104 25.185000000000002 23.669999999999998 29.154999999999998 21.990000000000002 105-109 25.169999999999998 23.474999999999998 29.909999999999997 21.445 110-114 23.885 23.965 30.17 21.98 115-119 24.154999999999998 25.09 29.03 21.725 120-124 23.330000000000002 26.150000000000002 28.1 22.42 125-129 23.135 25.765 29.035 22.065 130-134 23.21 25.86 28.694999999999997 22.235 135-139 23.09 25.195 30.19 21.525 140-144 23.775 24.245 30.735 21.245 145-149 23.175 23.94 30.380000000000003 22.505 150 24.275 23.275000000000002 28.725 23.724999999999998 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.5 20 1.0 21 1.0 22 2.0 23 2.5 24 6.0 25 10.5 26 10.5 27 6.5 28 5.5 29 14.5 30 25.0 31 35.5 32 41.0 33 38.5 34 51.5 35 78.5 36 121.0 37 176.0 38 212.0 39 217.5 40 212.0 41 231.5 42 226.0 43 216.5 44 233.0 45 231.0 46 211.0 47 146.5 48 109.5 49 96.5 50 82.0 51 72.5 52 43.5 53 37.5 54 39.5 55 39.0 56 37.0 57 29.5 58 34.0 59 43.0 60 44.0 61 36.0 62 33.5 63 52.5 64 62.5 65 59.5 66 44.0 67 30.0 68 34.0 69 26.0 70 20.0 71 23.5 72 21.0 73 15.5 74 13.5 75 17.0 76 15.0 77 8.5 78 5.0 79 1.5 80 1.0 81 2.0 82 1.0 83 0.0 84 1.0 85 1.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.875 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.125 #Duplication Level Percentage of deduplicated Percentage of total 1 79.16817359855335 54.725 2 13.056057866184448 18.05 3 3.580470162748644 7.425 4 1.5551537070524413 4.3 5 0.5063291139240507 1.7500000000000002 6 0.650994575045208 2.7 7 0.325497287522604 1.575 8 0.325497287522604 1.7999999999999998 9 0.25316455696202533 1.575 >10 0.5786618444846293 6.1 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 45 1.125 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 19 0.475 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 17 0.42500000000000004 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 15 0.375 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 15 0.375 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 14 0.35000000000000003 No Hit TGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATT 13 0.325 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 13 0.325 No Hit CTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTC 13 0.325 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 12 0.3 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 12 0.3 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 12 0.3 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 12 0.3 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 11 0.27499999999999997 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 11 0.27499999999999997 No Hit TGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACT 10 0.25 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 9 0.22499999999999998 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 9 0.22499999999999998 No Hit AGCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTG 9 0.22499999999999998 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 9 0.22499999999999998 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 9 0.22499999999999998 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 9 0.22499999999999998 No Hit GTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAAT 9 0.22499999999999998 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 8 0.2 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 8 0.2 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 8 0.2 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 8 0.2 No Hit AATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGC 8 0.2 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 8 0.2 No Hit TTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGA 8 0.2 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 8 0.2 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 8 0.2 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 7 0.17500000000000002 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 7 0.17500000000000002 No Hit GCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGC 7 0.17500000000000002 No Hit GACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCC 7 0.17500000000000002 No Hit AATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTAT 7 0.17500000000000002 No Hit AGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGG 7 0.17500000000000002 No Hit GCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGC 7 0.17500000000000002 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 7 0.17500000000000002 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 7 0.17500000000000002 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 6 0.15 No Hit ATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATG 6 0.15 No Hit GGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTC 6 0.15 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 6 0.15 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 6 0.15 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 6 0.15 No Hit TATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACC 6 0.15 No Hit GTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACT 6 0.15 No Hit GGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTA 6 0.15 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 6 0.15 No Hit GCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAG 6 0.15 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 6 0.15 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 6 0.15 No Hit TAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAG 6 0.15 No Hit ATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTG 6 0.15 No Hit CCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATA 6 0.15 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 6 0.15 No Hit GAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTA 6 0.15 No Hit TGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGA 5 0.125 No Hit AGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAA 5 0.125 No Hit ATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCT 5 0.125 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 5 0.125 No Hit GTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCT 5 0.125 No Hit TGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCA 5 0.125 No Hit CAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGC 5 0.125 No Hit CTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAG 5 0.125 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 5 0.125 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 5 0.125 No Hit CAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTA 5 0.125 No Hit TCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGG 5 0.125 No Hit GATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAGGAA 5 0.125 No Hit ATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.05 0.0 0.0 0.0 0.0 84-85 0.05 0.0 0.0 0.0 0.0 86-87 0.05 0.0 0.0 0.0 0.0 88-89 0.05 0.0 0.0 0.0 0.0 90-91 0.05 0.0 0.0 0.0 0.0 92-93 0.05 0.0 0.0 0.0 0.0 94-95 0.05 0.0 0.0 0.0 0.0 96-97 0.05 0.0 0.0 0.0 0.0 98-99 0.05 0.0 0.0 0.0 0.0 100-101 0.075 0.0 0.0 0.0 0.0 102-103 0.075 0.0 0.0 0.0 0.0 104-105 0.075 0.0 0.0 0.0 0.0 106-107 0.1 0.0 0.0 0.0 0.0 108-109 0.125 0.0 0.0 0.0 0.0 110-111 0.125 0.0 0.0 0.0 0.0 112-113 0.15 0.0 0.0 0.0 0.0 114-115 0.15 0.0 0.0 0.0 0.0 116-117 0.1875 0.0 0.0 0.0 0.0 118-119 0.2 0.0 0.0 0.0 0.0 120-121 0.2625 0.0 0.0 0.0 0.0 122-123 0.325 0.0 0.0 0.0 0.0 124-125 0.3625 0.0 0.0 0.0 0.0 126-127 0.375 0.0 0.0 0.0 0.0 128-129 0.4125 0.0 0.0 0.0 0.0 130-131 0.5 0.0 0.0 0.0 0.0 132-133 0.5375000000000001 0.0 0.0 0.0 0.0 134-135 0.5874999999999999 0.0 0.0 0.0 0.0 136-137 0.6125 0.0 0.0 0.0 0.0 138 0.625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 2833680 spots for ERR11006580.sra Written 2833680 spots for ERR11006580.sra Read 2833680 spots for ERR11006580.sra Written 2833680 spots for ERR11006580.sra Read 2833680 spots for ERR11006580.sra Written 2833680 spots for ERR11006580.sra Read 2833680 spots for ERR11006580.sra Written 2833680 spots for ERR11006580.sra Read 2833680 spots for ERR11006580.sra Written 2833680 spots for ERR11006580.sra Read 2833680 spots for ERR11006580.sra Written 2833680 spots for ERR11006580.sra Read 2833680 spots for ERR11006580.sra Written 2833680 spots for ERR11006580.sra Read 2833680 spots for ERR11006580.sra Written 2833680 spots for ERR11006580.sra Read 2833680 spots for ERR11006580.sra Written 2833680 spots for ERR11006580.sra Read 2833680 spots for ERR11006580.sra Written 2833680 spots for ERR11006580.sra Read 2833680 spots for ERR11006580.sra Written 2833680 spots for ERR11006580.sra Read 2833680 spots for ERR11006580.sra Written 2833680 spots for ERR11006580.sra Read 2833680 spots for ERR11006580.sra Written 2833680 spots for ERR11006580.sra Read 2833680 spots for ERR11006580.sra Written 2833680 spots for ERR11006580.sra Read 2833680 spots for ERR11006580.sra Written 2833680 spots for ERR11006580.sra Read 2833680 spots for ERR11006580.sra Written 2833680 spots for ERR11006580.sra Read 2833680 spots for ERR11006580.sra Written 2833680 spots for ERR11006580.sra Read 2833680 spots for ERR11006580.sra Written 2833680 spots for ERR11006580.sra Read 2833680 spots for ERR11006580.sra Written 2833680 spots for ERR11006580.sra Read 2833695 spots for ERR11006580.sra Written 2833695 spots for ERR11006580.sra SRR ids: ['ERR11006580.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_nre96o8g ERR11006580.sra spots: 56673615 blocks: [[1, 2833680], [2833681, 5667360], [5667361, 8501040], [8501041, 11334720], [11334721, 14168400], [14168401, 17002080], [17002081, 19835760], [19835761, 22669440], [22669441, 25503120], [25503121, 28336800], [28336801, 31170480], [31170481, 34004160], [34004161, 36837840], [36837841, 39671520], [39671521, 42505200], [42505201, 45338880], [45338881, 48172560], [48172561, 51006240], [51006241, 53839920], [53839921, 56673615]] ERR11006580 file size 20824781 ERR11006580 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006580 ERR11006580_1.fastq ERR11006580_2.fastq Input file: ERR11006580_1.fastq Paired file: ERR11006580_2.fastq trimmed: ERR11006580-trimmed-pair1.fastq, ERR11006580-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 17:56:37 2024 >> started Fri Dec 6 17:58:01 2024 >> done (84.431s) 56673615 read pairs processed; of these: 122 ( 0.00%) short read pairs filtered out after trimming by size control 687 ( 0.00%) empty read pairs filtered out after trimming by size control 56672806 (100.00%) read pairs available; of these: 578121 ( 1.02%) trimmed read pairs available after processing 56094685 (98.98%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 4 0.00% 20 8 0.00% 21 7 0.00% 22 24 0.00% 23 7 0.00% 24 3 0.00% 25 41 0.00% 26 9 0.00% 27 22 0.00% 28 17 0.00% 29 24 0.00% 30 15 0.00% 31 79 0.00% 32 13 0.00% 33 12 0.00% 34 11 0.00% 35 14 0.00% 36 15 0.00% 37 19 0.00% 38 22 0.00% 39 29 0.00% 40 31 0.00% 41 20 0.00% 42 29 0.00% 43 24 0.00% 44 35 0.00% 45 16 0.00% 46 29 0.00% 47 38 0.00% 48 39 0.00% 49 41 0.00% 50 40 0.00% 51 57 0.00% 52 73 0.00% 53 64 0.00% 54 86 0.00% 55 81 0.00% 56 78 0.00% 57 118 0.00% 58 101 0.00% 59 118 0.00% 60 124 0.00% 61 153 0.00% 62 165 0.00% 63 176 0.00% 64 179 0.00% 65 250 0.00% 66 221 0.00% 67 230 0.00% 68 254 0.00% 69 244 0.00% 70 288 0.00% 71 309 0.00% 72 357 0.00% 73 406 0.00% 74 414 0.00% 75 465 0.00% 76 486 0.00% 77 519 0.00% 78 588 0.00% 79 606 0.00% 80 632 0.00% 81 691 0.00% 82 772 0.00% 83 835 0.00% 84 901 0.00% 85 1047 0.00% 86 1087 0.00% 87 1197 0.00% 88 1178 0.00% 89 1275 0.00% 90 1413 0.00% 91 1475 0.00% 92 1456 0.00% 93 1600 0.00% 94 1709 0.00% 95 1834 0.00% 96 1927 0.00% 97 2031 0.00% 98 2403 0.00% 99 2387 0.00% 100 2422 0.00% 101 2679 0.00% 102 2768 0.00% 103 2983 0.01% 104 3063 0.01% 105 3225 0.01% 106 3559 0.01% 107 3740 0.01% 108 3921 0.01% 109 4063 0.01% 110 4410 0.01% 111 4557 0.01% 112 4909 0.01% 113 5043 0.01% 114 5217 0.01% 115 5520 0.01% 116 5993 0.01% 117 6429 0.01% 118 6359 0.01% 119 6748 0.01% 120 7560 0.01% 121 7848 0.01% 122 8400 0.01% 123 8727 0.02% 124 9101 0.02% 125 10328 0.02% 126 9658 0.02% 127 9358 0.02% 128 10481 0.02% 129 11103 0.02% 130 11954 0.02% 131 12736 0.02% 132 13386 0.02% 133 12985 0.02% 134 13525 0.02% 135 13468 0.02% 136 14366 0.03% 137 14878 0.03% 138 15781 0.03% 139 16695 0.03% 140 17416 0.03% 141 18542 0.03% 142 19435 0.03% 143 20216 0.04% 144 21002 0.04% 145 21964 0.04% 146 25721 0.05% 147 24789 0.04% 148 26016 0.05% 149 27271 0.05% 150 56094685 98.98% 56672806 reads passed initial QC criterion=sequence-density sequence-density=0.29 sequence-density-rank=1 fanout-score=0.00 fanout-score-rank=33 prefix-density=0.00 prefix-fanout=1.0 sequence=GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTTATCCTTCCACCGTTGGAAGCGGGCAGTTGTCGCTGCTCTGTGAAGCCAGCCTCACGCTGTGCCTGCCAACATTATGGGCCGCGAAGCCTAGCTTTCGCTTAAGCTCCAACGGCCCACTACGCAACTTGGAACGGGCGGGCCATCAGTAGCACACCCAGACCAGGCCCGCAGCGCTACGGCAACGTCCACACCACCCTTAAAGCCCCCACT criterion=fanout-score sequence-density=0.01 sequence-density-rank=30 fanout-score=639.43 fanout-score-rank=1 prefix-density=6.28 prefix-fanout=1.0 sequence=AAGTATTAATACAGTCACATGGATGGTAAATGCGTGAATGTGATGGACTAAAAAATCTGCAGTTCCTAATGGAATAGGTAACAAAGCCACTTTGCCGCCTACTGCTACTAACTCGCCACCTCCCCACGTTAAACTGGTACTTGTTGTTGCACCAGGAGCTGTTACACCAGGCGCGTTAGCATGGATATTTTGTACCCATTGAGCAAAGATAGGTTGTAATTGTATGGCAGTATCCGAAAACATATCTTGCGGACGTCCTAAAGCACTCATGGTATCATTATGAATGTACAAACCAAAACTGTGAAAACCTAGAAATATACATACCCAGTTAAGGTGGGATATGATTGCATCACGGTGTCTAAGGACGCGATCTAATAGATCATTGTATCGAGTAGTTGGATCATAGTCTCTTACCATAAAAATTGCTGCATGTGCAGCAGCACCGACTATTAGAAATCCGCCAATCCACATGTGGTGTGTGAACAAGGAAAGTTGTGTACCATAGTCAGTA criterion=sequence-density sequence-density=0.32 sequence-density-rank=1 fanout-score=10.62 fanout-score-rank=7 prefix-density=3.40 prefix-fanout=1.0 sequence=TTGCGTAGTGGATCTGCTGGGGCCTATGCGAAAGCTGGGCCTCACGAATTCTATAGTGGCAGGCACCGCGTTAGGCTGGCTTC criterion=fanout-score sequence-density=0.08 sequence-density-rank=18 fanout-score=125.20 fanout-score-rank=1 prefix-density=9.49 prefix-fanout=1.0 sequence=CTGGATAACTATCACTGAAAATC ERR11006580 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 17:58:41 Started mapping on | Dec 06 17:58:41 Finished on | Dec 06 18:05:20 Mapping speed, Million of reads per hour | 511.33 Number of input reads | 56672806 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 44318382 Uniquely mapped reads % | 78.20% Average mapped length | 298.37 Number of splices: Total | 16928002 Number of splices: Annotated (sjdb) | 15843976 Number of splices: GT/AG | 16592171 Number of splices: GC/AG | 200121 Number of splices: AT/AC | 19144 Number of splices: Non-canonical | 116566 Mismatch rate per base, % | 0.30% Deletion rate per base | 0.02% Deletion average length | 1.72 Insertion rate per base | 0.01% Insertion average length | 2.07 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 10245065 % of reads mapped to multiple loci | 18.08% Number of reads mapped to too many loci | 11950 % of reads mapped to too many loci | 0.02% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.93% % of reads unmapped: other | 0.77% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2109359 2109359 2109359 N_multimapping 10245065 10245065 10245065 N_noFeature 10458480 41032113 12803354 N_ambiguous 1478320 43839 529044 UnstrandedReadsAssigned:32381582 PositiveStrandReadsAssigned:3242430 NegativeStrandReadsAssigned:30985984 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006580 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006580-trimmed-pair1.fastq ERR11006580-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 56,672,806 reads, 33,341,074 reads pseudoaligned [quant] estimated average fragment length: 261.703 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,254 rounds 52973 ERR11006580.ke.tsv 35125 ERR11006580.se.tsv 88098 total ==> ERR11006580.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 675.599 0 0 PNS24247 1044 783.297 29.5075 1.17054 PNS24249 1928 1667.3 194.428 3.62348 PNS24246 1044 783.297 29.5075 1.17054 PNS24248 1044 783.297 29.5075 1.17054 PNS24244 1471 1210.3 79.0498 2.0295 PNS24243 293 56.6386 0 0 KQK14069 1603 1342.3 786.145 18.1985 KQK14071 474 215.528 25.7773 3.71633 ==> ERR11006580.se.tsv <== BRADI_1g14170v3 925 BRADI_1g53295v3 427 BRADI_1g59795v3 481 BRADI_1g07683v3 0 BRADI_1g00485v3 10 BRADI_1g20270v3 445 BRADI_1g74790v3 360 BRADI_1g09890v3 0 BRADI_1g77505v3 225 BRADI_1g48960v3 0 ERR11006580 completed mapping pipeline successfully