Starting /dee2/code/volunteer_pipeline.sh ERR11006581 current disk space = 1550531444736 free memory = 1401283596 ERR11006581 SRAfilesize 969a0aba7a8e4f8b30bb0c661eaa4052 ERR11006581.sra ERR11006581.sra file validated ERR11006581 is paired end ERR11006581 is conventional basespace ERR11006581 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006581_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.352 37.0 37.0 37.0 37.0 37.0 2 36.213 37.0 37.0 37.0 37.0 37.0 3 36.2875 37.0 37.0 37.0 37.0 37.0 4 36.381 37.0 37.0 37.0 37.0 37.0 5 36.378 37.0 37.0 37.0 37.0 37.0 6 36.38 37.0 37.0 37.0 37.0 37.0 7 36.3415 37.0 37.0 37.0 37.0 37.0 8 36.256 37.0 37.0 37.0 37.0 37.0 9 36.403 37.0 37.0 37.0 37.0 37.0 10-14 36.4045 37.0 37.0 37.0 37.0 37.0 15-19 36.395500000000006 37.0 37.0 37.0 37.0 37.0 20-24 36.339099999999995 37.0 37.0 37.0 37.0 37.0 25-29 36.2592 37.0 37.0 37.0 37.0 37.0 30-34 36.2307 37.0 37.0 37.0 37.0 37.0 35-39 36.182500000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.2141 37.0 37.0 37.0 37.0 37.0 45-49 36.153200000000005 37.0 37.0 37.0 37.0 37.0 50-54 36.11149999999999 37.0 37.0 37.0 37.0 37.0 55-59 36.0918 37.0 37.0 37.0 37.0 37.0 60-64 36.041399999999996 37.0 37.0 37.0 37.0 37.0 65-69 36.019 37.0 37.0 37.0 37.0 37.0 70-74 36.029399999999995 37.0 37.0 37.0 37.0 37.0 75-79 36.0032 37.0 37.0 37.0 37.0 37.0 80-84 35.9828 37.0 37.0 37.0 37.0 37.0 85-89 35.8976 37.0 37.0 37.0 37.0 37.0 90-94 35.8451 37.0 37.0 37.0 37.0 37.0 95-99 35.9033 37.0 37.0 37.0 37.0 37.0 100-104 35.8727 37.0 37.0 37.0 37.0 37.0 105-109 35.7153 37.0 37.0 37.0 37.0 37.0 110-114 35.6284 37.0 37.0 37.0 37.0 37.0 115-119 35.6376 37.0 37.0 37.0 37.0 37.0 120-124 35.662699999999994 37.0 37.0 37.0 37.0 37.0 125-129 35.515 37.0 37.0 37.0 37.0 37.0 130-134 35.4017 37.0 37.0 37.0 37.0 37.0 135-139 35.348 37.0 37.0 37.0 34.6 37.0 140-144 35.1086 37.0 37.0 37.0 25.0 37.0 145-149 35.2874 37.0 37.0 37.0 34.6 37.0 150 35.2595 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 5.0 24 2.0 25 6.0 26 17.0 27 21.0 28 18.0 29 41.0 30 46.0 31 58.0 32 86.0 33 113.0 34 180.0 35 343.0 36 2760.0 37 304.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.35 12.55 16.025 34.075 2 30.025000000000002 10.424999999999999 25.55 34.0 3 23.65 13.325000000000001 28.125 34.9 4 28.725 15.125 24.8 31.35 5 30.0 19.125 25.974999999999998 24.9 6 24.975 29.375 19.825 25.825 7 17.424999999999997 32.225 31.674999999999997 18.675 8 18.0 30.275000000000002 32.975 18.75 9 18.099999999999998 27.6 34.625 19.675 10-14 20.215 32.515 26.765 20.505000000000003 15-19 20.705000000000002 34.58 24.095 20.62 20-24 19.55 28.615000000000002 28.1 23.735 25-29 22.74 31.865 24.62 20.775 30-34 24.035 30.380000000000003 24.715 20.87 35-39 22.585 31.919999999999998 25.06 20.435 40-44 20.7 31.005 24.75 23.544999999999998 45-49 20.215 29.459999999999997 27.04 23.285 50-54 21.315 32.16 25.345000000000002 21.18 55-59 22.384999999999998 30.055 23.34 24.22 60-64 19.900000000000002 31.825 25.03 23.244999999999997 65-69 20.665 30.56 24.62 24.154999999999998 70-74 23.875 30.055 21.36 24.709999999999997 75-79 23.07 30.159999999999997 24.68 22.09 80-84 22.865 29.825000000000003 24.245 23.064999999999998 85-89 24.32 28.694999999999997 25.165 21.82 90-94 21.41 29.24 26.974999999999998 22.375 95-99 25.035 28.999999999999996 22.875 23.09 100-104 22.365 30.48 23.565 23.59 105-109 22.3 28.7 26.02 22.98 110-114 23.615 28.449999999999996 24.715 23.22 115-119 20.375 30.869999999999997 25.509999999999998 23.244999999999997 120-124 19.255 30.85 24.77 25.124999999999996 125-129 21.135 32.355000000000004 21.47 25.040000000000003 130-134 23.189999999999998 30.43 24.665 21.715 135-139 23.98 29.744999999999997 22.625 23.65 140-144 24.665 28.78 25.650000000000002 20.905 145-149 23.115 30.2 24.54 22.145 150 22.95 27.150000000000002 28.125 21.775 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 1.0 19 1.0 20 1.0 21 1.5 22 1.5 23 0.5 24 1.5 25 4.5 26 4.5 27 6.5 28 10.0 29 12.5 30 20.0 31 22.5 32 25.5 33 30.0 34 35.0 35 52.0 36 81.5 37 150.5 38 288.0 39 304.5 40 250.0 41 305.5 42 291.5 43 244.0 44 223.5 45 205.0 46 200.0 47 164.5 48 106.5 49 66.5 50 65.5 51 55.5 52 39.0 53 35.5 54 33.5 55 34.0 56 30.5 57 22.5 58 21.0 59 24.0 60 29.5 61 28.0 62 21.5 63 27.0 64 62.5 65 87.0 66 51.5 67 19.5 68 24.5 69 21.0 70 17.0 71 24.0 72 22.0 73 18.0 74 15.5 75 14.0 76 13.5 77 8.5 78 5.5 79 4.0 80 3.5 81 3.5 82 1.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.324999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 80.43212392988178 49.325 2 11.82225845902976 14.499999999999998 3 2.6498165511618423 4.875 4 1.5898899306971057 3.9 5 0.6522625356706074 2.0 6 0.5707297187117816 2.1 7 0.40766408479412963 1.7500000000000002 8 0.16306563391765186 0.8 9 0.16306563391765186 0.8999999999999999 >10 1.5083571137382796 16.5 >50 0.0 0.0 >100 0.040766408479412965 3.35 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 134 3.35 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 39 0.975 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 34 0.8500000000000001 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 34 0.8500000000000001 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 30 0.75 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 30 0.75 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 27 0.675 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 24 0.6 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 23 0.575 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 23 0.575 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 22 0.5499999999999999 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 21 0.525 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 20 0.5 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 19 0.475 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 18 0.44999999999999996 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 18 0.44999999999999996 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 17 0.42500000000000004 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 16 0.4 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 15 0.375 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 15 0.375 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 15 0.375 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 14 0.35000000000000003 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 14 0.35000000000000003 No Hit TAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGA 14 0.35000000000000003 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 13 0.325 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 13 0.325 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 12 0.3 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 12 0.3 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 12 0.3 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 11 0.27499999999999997 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 11 0.27499999999999997 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 11 0.27499999999999997 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 11 0.27499999999999997 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 11 0.27499999999999997 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 11 0.27499999999999997 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 10 0.25 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 10 0.25 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 10 0.25 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 9 0.22499999999999998 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 9 0.22499999999999998 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 9 0.22499999999999998 No Hit GGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGG 9 0.22499999999999998 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 8 0.2 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 8 0.2 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 8 0.2 No Hit GTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCG 8 0.2 No Hit TGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 7 0.17500000000000002 No Hit TGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGA 7 0.17500000000000002 No Hit ATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATA 7 0.17500000000000002 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 7 0.17500000000000002 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 7 0.17500000000000002 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 7 0.17500000000000002 No Hit CATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAA 7 0.17500000000000002 No Hit GGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 7 0.17500000000000002 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 7 0.17500000000000002 No Hit CACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAAT 7 0.17500000000000002 No Hit TCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCG 6 0.15 No Hit CGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 6 0.15 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 6 0.15 No Hit GAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAG 6 0.15 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 6 0.15 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 6 0.15 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 6 0.15 No Hit GGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAG 6 0.15 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 6 0.15 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 6 0.15 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 6 0.15 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 6 0.15 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 6 0.15 No Hit CACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCG 6 0.15 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 5 0.125 No Hit GCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCC 5 0.125 No Hit TCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGT 5 0.125 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 5 0.125 No Hit TTCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCT 5 0.125 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 5 0.125 No Hit CAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGA 5 0.125 No Hit TGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCA 5 0.125 No Hit GAGCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTA 5 0.125 No Hit CGGCACACGTCCCTGACTCCCGTCTCCGTCATCCGCTTCACGAAGGCGGC 5 0.125 No Hit AACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAG 5 0.125 No Hit ACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAG 5 0.125 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 5 0.125 No Hit CGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTC 5 0.125 No Hit AGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGG 5 0.125 No Hit CTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0125 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.05 0.0 0.0 0.0 0.0 92-93 0.05 0.0 0.0 0.0 0.0 94-95 0.05 0.0 0.0 0.0 0.0 96-97 0.075 0.0 0.0 0.0 0.0 98-99 0.125 0.0 0.0 0.0 0.0 100-101 0.125 0.0 0.0 0.0 0.0 102-103 0.125 0.0 0.0 0.0 0.0 104-105 0.125 0.0 0.0 0.0 0.0 106-107 0.125 0.0 0.0 0.0 0.0 108-109 0.125 0.0 0.0 0.0 0.0 110-111 0.125 0.0 0.0 0.0 0.0 112-113 0.125 0.0 0.0 0.0 0.0 114-115 0.125 0.0 0.0 0.0 0.0 116-117 0.125 0.0 0.0 0.0 0.0 118-119 0.1875 0.0 0.0 0.0 0.0 120-121 0.2375 0.0 0.0 0.0 0.0 122-123 0.2625 0.0 0.0 0.0 0.0 124-125 0.275 0.0 0.0 0.0 0.0 126-127 0.3625 0.0 0.0 0.0 0.0 128-129 0.3875 0.0 0.0 0.0 0.0 130-131 0.4625 0.0 0.0 0.0 0.0 132-133 0.5 0.0 0.0 0.0 0.0 134-135 0.5375000000000001 0.0 0.0 0.0 0.0 136-137 0.5874999999999999 0.0 0.0 0.0 0.0 138 0.6 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCTTCA 30 9.508585E-8 120.0 9 CTTTTCT 35 2.3845314E-7 102.85714 6 TTTCTTC 35 2.3845314E-7 102.85714 8 GCTTTCT 35 2.3845314E-7 102.85714 1 CTTTCTT 35 2.3845314E-7 102.85714 2 TCTTTTC 40 5.2840005E-7 90.0 5 TTTCTTT 45 1.0653384E-6 80.0 3 TTCTTTT 45 1.0653384E-6 80.0 4 TTTTCTT 50 1.9936197E-6 72.0 7 TATATGT 30 4.3922482E-5 28.8 20-24 AATTCTT 30 4.3922482E-5 28.8 15-19 TGTTAGC 30 4.3922482E-5 28.8 25-29 CTTATAT 30 4.3922482E-5 28.8 20-24 GTTAGCG 30 4.3922482E-5 28.8 25-29 CTTCAAA 35 1.2542517E-4 24.685713 10-14 AAATTCT 35 1.2542517E-4 24.685713 15-19 TTCAAAA 35 1.2542517E-4 24.685713 10-14 TATGTTA 35 1.2542517E-4 24.685713 25-29 TTATATG 35 1.2542517E-4 24.685713 20-24 ATGTTAG 35 1.2542517E-4 24.685713 25-29 >>END_MODULE ERR11006581 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006581_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.04625 37.0 37.0 37.0 37.0 37.0 2 35.378 37.0 37.0 37.0 37.0 37.0 3 35.6465 37.0 37.0 37.0 37.0 37.0 4 35.609 37.0 37.0 37.0 37.0 37.0 5 35.87 37.0 37.0 37.0 37.0 37.0 6 35.8825 37.0 37.0 37.0 37.0 37.0 7 35.825 37.0 37.0 37.0 37.0 37.0 8 35.8735 37.0 37.0 37.0 37.0 37.0 9 35.971 37.0 37.0 37.0 37.0 37.0 10-14 35.939299999999996 37.0 37.0 37.0 37.0 37.0 15-19 35.8889 37.0 37.0 37.0 37.0 37.0 20-24 35.81439999999999 37.0 37.0 37.0 37.0 37.0 25-29 35.8371 37.0 37.0 37.0 37.0 37.0 30-34 35.8237 37.0 37.0 37.0 37.0 37.0 35-39 35.715199999999996 37.0 37.0 37.0 37.0 37.0 40-44 35.7781 37.0 37.0 37.0 37.0 37.0 45-49 35.7313 37.0 37.0 37.0 37.0 37.0 50-54 35.705400000000004 37.0 37.0 37.0 37.0 37.0 55-59 35.5818 37.0 37.0 37.0 37.0 37.0 60-64 35.446200000000005 37.0 37.0 37.0 37.0 37.0 65-69 35.4987 37.0 37.0 37.0 37.0 37.0 70-74 35.434099999999994 37.0 37.0 37.0 37.0 37.0 75-79 35.4097 37.0 37.0 37.0 37.0 37.0 80-84 35.325199999999995 37.0 37.0 37.0 37.0 37.0 85-89 35.3093 37.0 37.0 37.0 32.2 37.0 90-94 35.3012 37.0 37.0 37.0 32.2 37.0 95-99 35.0144 37.0 37.0 37.0 27.4 37.0 100-104 35.0218 37.0 37.0 37.0 25.0 37.0 105-109 34.9323 37.0 37.0 37.0 25.0 37.0 110-114 34.8765 37.0 37.0 37.0 25.0 37.0 115-119 34.8229 37.0 37.0 37.0 25.0 37.0 120-124 34.6238 37.0 37.0 37.0 25.0 37.0 125-129 34.7122 37.0 37.0 37.0 25.0 37.0 130-134 34.5594 37.0 37.0 37.0 25.0 37.0 135-139 34.427200000000006 37.0 37.0 37.0 25.0 37.0 140-144 34.328 37.0 37.0 37.0 25.0 37.0 145-149 34.4593 37.0 37.0 37.0 25.0 37.0 150 33.9365 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 1.0 19 0.0 20 0.0 21 2.0 22 2.0 23 7.0 24 16.0 25 17.0 26 25.0 27 25.0 28 34.0 29 44.0 30 55.0 31 68.0 32 110.0 33 175.0 34 345.0 35 997.0 36 2015.0 37 61.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.089053329931104 25.184996172492983 18.09134983414136 27.63460066343455 2 26.450000000000003 22.825 34.225 16.5 3 20.925 26.075 34.175 18.825 4 22.625 27.3 29.125 20.95 5 22.875 28.599999999999998 30.075000000000003 18.45 6 21.85 31.574999999999996 28.775000000000002 17.8 7 20.150000000000002 21.125 40.525 18.2 8 22.625 22.05 31.075000000000003 24.25 9 22.95 19.35 35.65 22.05 10-14 23.455000000000002 26.295 28.74 21.51 15-19 24.245 24.385 30.404999999999998 20.965 20-24 24.785 23.86 30.505 20.849999999999998 25-29 23.57 23.935000000000002 31.319999999999997 21.175 30-34 25.365 24.055 30.455 20.125 35-39 24.560000000000002 23.98 29.970000000000002 21.490000000000002 40-44 22.585 25.795 29.93 21.69 45-49 23.0 26.295 29.160000000000004 21.545 50-54 23.265 26.05 28.525 22.16 55-59 23.380000000000003 24.560000000000002 28.860000000000003 23.200000000000003 60-64 22.235 25.3 29.635 22.830000000000002 65-69 24.085 24.775 28.694999999999997 22.445 70-74 24.485 24.154999999999998 30.490000000000002 20.87 75-79 24.09 24.455 30.145 21.310000000000002 80-84 24.965 24.375 29.635 21.025 85-89 24.69 25.22 27.675 22.415 90-94 23.28 24.455 29.439999999999998 22.825 95-99 23.535 24.26 29.715000000000003 22.49 100-104 24.605 23.599999999999998 30.245 21.55 105-109 25.505 23.26 30.159999999999997 21.075 110-114 24.169999999999998 23.395 29.735 22.7 115-119 23.86 25.055 29.189999999999998 21.895 120-124 23.735 24.825 28.95 22.49 125-129 22.595000000000002 25.36 29.995 22.05 130-134 22.775000000000002 26.369999999999997 28.57 22.285 135-139 23.974999999999998 25.230000000000004 29.830000000000002 20.965 140-144 23.345 25.365 29.685 21.605 145-149 22.43 24.51 30.735 22.325 150 22.900000000000002 22.5 30.45 24.15 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 1.5 18 1.5 19 0.5 20 0.5 21 0.5 22 0.5 23 1.0 24 4.0 25 8.0 26 9.0 27 15.0 28 18.5 29 18.0 30 30.0 31 36.5 32 35.0 33 40.5 34 68.5 35 94.5 36 124.5 37 189.5 38 220.5 39 208.0 40 217.5 41 252.5 42 240.5 43 226.0 44 233.0 45 209.0 46 180.0 47 147.0 48 115.0 49 83.5 50 57.5 51 49.0 52 41.5 53 36.5 54 40.0 55 35.5 56 26.0 57 28.5 58 34.5 59 32.0 60 32.5 61 34.0 62 34.5 63 55.0 64 75.5 65 67.5 66 40.5 67 20.0 68 20.0 69 25.5 70 32.0 71 29.5 72 23.0 73 24.0 74 20.5 75 13.0 76 7.5 77 6.0 78 5.5 79 4.5 80 5.0 81 4.5 82 3.5 83 2.5 84 2.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.95 #Duplication Level Percentage of deduplicated Percentage of total 1 79.6057619408643 52.5 2 11.940864291129644 15.75 3 3.222137983320698 6.375 4 1.6679302501895377 4.3999999999999995 5 0.9476876421531463 3.125 6 0.7202426080363912 2.85 7 0.4169825625473844 1.925 8 0.37907505686125853 2.0 9 0.22744503411675512 1.35 >10 0.8718726307808946 9.725 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 50 1.25 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 26 0.65 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 26 0.65 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 22 0.5499999999999999 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 21 0.525 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 18 0.44999999999999996 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 18 0.44999999999999996 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 17 0.42500000000000004 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 16 0.4 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 16 0.4 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 16 0.4 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 16 0.4 No Hit GAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCG 15 0.375 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 14 0.35000000000000003 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 14 0.35000000000000003 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 12 0.3 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 12 0.3 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 10 0.25 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 10 0.25 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 10 0.25 No Hit AATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGC 10 0.25 No Hit GCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGC 10 0.25 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 10 0.25 No Hit TGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGA 9 0.22499999999999998 No Hit GATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTC 9 0.22499999999999998 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 9 0.22499999999999998 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 9 0.22499999999999998 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 9 0.22499999999999998 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 9 0.22499999999999998 No Hit GGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTC 8 0.2 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 8 0.2 No Hit GAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAAT 8 0.2 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 8 0.2 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 8 0.2 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 8 0.2 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 8 0.2 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 8 0.2 No Hit TCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTG 8 0.2 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 8 0.2 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 7 0.17500000000000002 No Hit AGCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTG 7 0.17500000000000002 No Hit CTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAG 7 0.17500000000000002 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 7 0.17500000000000002 No Hit CTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTG 7 0.17500000000000002 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 7 0.17500000000000002 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 7 0.17500000000000002 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 7 0.17500000000000002 No Hit TGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACT 7 0.17500000000000002 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 7 0.17500000000000002 No Hit ATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTG 7 0.17500000000000002 No Hit CTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGA 6 0.15 No Hit CTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCG 6 0.15 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 6 0.15 No Hit TTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATAC 6 0.15 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 6 0.15 No Hit TTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGA 6 0.15 No Hit ATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCAC 6 0.15 No Hit AATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTAT 6 0.15 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 6 0.15 No Hit CGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCC 6 0.15 No Hit CTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAG 6 0.15 No Hit ATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGA 6 0.15 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 6 0.15 No Hit TTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGG 6 0.15 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 6 0.15 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 6 0.15 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 6 0.15 No Hit CTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAA 6 0.15 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 6 0.15 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 5 0.125 No Hit ACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAG 5 0.125 No Hit ATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCT 5 0.125 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 5 0.125 No Hit GTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTT 5 0.125 No Hit GGGTGGTGTGGACGTTGCCGTAGCGCTGCGGGCCTGGTCTGGGTGTGCTA 5 0.125 No Hit CGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGT 5 0.125 No Hit AAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAG 5 0.125 No Hit CGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGAC 5 0.125 No Hit TGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATA 5 0.125 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 5 0.125 No Hit TGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCA 5 0.125 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 5 0.125 No Hit ATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGATGGTATTC 5 0.125 No Hit ATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGT 5 0.125 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 5 0.125 No Hit TTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGG 5 0.125 No Hit CCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCAG 5 0.125 No Hit GTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGA 5 0.125 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 5 0.125 No Hit AATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATG 5 0.125 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 5 0.125 No Hit GAGAACCTGGTGCAGCAGGTGGCCAACGCGGCGAAGGAGGCCGGCATCGG 5 0.125 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 5 0.125 No Hit CTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0125 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.05 0.0 0.0 0.0 0.0 92-93 0.05 0.0 0.0 0.0 0.0 94-95 0.05 0.0 0.0 0.0 0.0 96-97 0.075 0.0 0.0 0.0 0.0 98-99 0.125 0.0 0.0 0.0 0.0 100-101 0.125 0.0 0.0 0.0 0.0 102-103 0.125 0.0 0.0 0.0 0.0 104-105 0.125 0.0 0.0 0.0 0.0 106-107 0.125 0.0 0.0 0.0 0.0 108-109 0.125 0.0 0.0 0.0 0.0 110-111 0.125 0.0 0.0 0.0 0.0 112-113 0.125 0.0 0.0 0.0 0.0 114-115 0.125 0.0 0.0 0.0 0.0 116-117 0.125 0.0 0.0 0.0 0.0 118-119 0.1875 0.0 0.0 0.0 0.0 120-121 0.2375 0.0 0.0 0.0 0.0 122-123 0.2625 0.0 0.0 0.0 0.0 124-125 0.275 0.0 0.0 0.0 0.0 126-127 0.3625 0.0 0.0 0.0 0.0 128-129 0.3875 0.0 0.0 0.0 0.0 130-131 0.4625 0.0 0.0 0.0 0.0 132-133 0.5 0.0 0.0 0.0 0.0 134-135 0.5375000000000001 0.0 0.0 0.0 0.0 136-137 0.65 0.0 0.0 0.0 0.0 138 0.675 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 2573138 spots for ERR11006581.sra Written 2573138 spots for ERR11006581.sra Read 2573138 spots for ERR11006581.sra Written 2573138 spots for ERR11006581.sra Read 2573155 spots for ERR11006581.sra Written 2573155 spots for ERR11006581.sra Read 2573138 spots for ERR11006581.sra Written 2573138 spots for ERR11006581.sra Read 2573138 spots for ERR11006581.sra Written 2573138 spots for ERR11006581.sra Read 2573138 spots for ERR11006581.sra Written 2573138 spots for ERR11006581.sra Read 2573138 spots for ERR11006581.sra Written 2573138 spots for ERR11006581.sra Read 2573138 spots for ERR11006581.sra Written 2573138 spots for ERR11006581.sra Read 2573138 spots for ERR11006581.sra Written 2573138 spots for ERR11006581.sra Read 2573138 spots for ERR11006581.sra Written 2573138 spots for ERR11006581.sra Read 2573138 spots for ERR11006581.sra Written 2573138 spots for ERR11006581.sra Read 2573138 spots for ERR11006581.sra Written 2573138 spots for ERR11006581.sra Read 2573138 spots for ERR11006581.sra Written 2573138 spots for ERR11006581.sra Read 2573138 spots for ERR11006581.sra Written 2573138 spots for ERR11006581.sra Read 2573138 spots for ERR11006581.sra Written 2573138 spots for ERR11006581.sra Read 2573138 spots for ERR11006581.sra Written 2573138 spots for ERR11006581.sra Read 2573138 spots for ERR11006581.sra Written 2573138 spots for ERR11006581.sra Read 2573138 spots for ERR11006581.sra Written 2573138 spots for ERR11006581.sra Read 2573138 spots for ERR11006581.sra Written 2573138 spots for ERR11006581.sra Read 2573138 spots for ERR11006581.sra Written 2573138 spots for ERR11006581.sra SRR ids: ['ERR11006581.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_dogcj_04 ERR11006581.sra spots: 51462777 blocks: [[1, 2573138], [2573139, 5146276], [5146277, 7719414], [7719415, 10292552], [10292553, 12865690], [12865691, 15438828], [15438829, 18011966], [18011967, 20585104], [20585105, 23158242], [23158243, 25731380], [25731381, 28304518], [28304519, 30877656], [30877657, 33450794], [33450795, 36023932], [36023933, 38597070], [38597071, 41170208], [41170209, 43743346], [43743347, 46316484], [46316485, 48889622], [48889623, 51462777]] ERR11006581 file size 18909056 ERR11006581 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006581 ERR11006581_1.fastq ERR11006581_2.fastq Input file: ERR11006581_1.fastq Paired file: ERR11006581_2.fastq trimmed: ERR11006581-trimmed-pair1.fastq, ERR11006581-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 17:57:18 2024 >> started Fri Dec 6 17:58:31 2024 >> done (73.087s) 51462777 read pairs processed; of these: 214 ( 0.00%) short read pairs filtered out after trimming by size control 2382 ( 0.00%) empty read pairs filtered out after trimming by size control 51460181 (99.99%) read pairs available; of these: 376864 ( 0.73%) trimmed read pairs available after processing 51083317 (99.27%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 12 0.00% 19 10 0.00% 20 8 0.00% 21 13 0.00% 22 36 0.00% 23 16 0.00% 24 5 0.00% 25 30 0.00% 26 10 0.00% 27 16 0.00% 28 23 0.00% 29 26 0.00% 30 22 0.00% 31 81 0.00% 32 29 0.00% 33 14 0.00% 34 12 0.00% 35 17 0.00% 36 29 0.00% 37 17 0.00% 38 17 0.00% 39 21 0.00% 40 18 0.00% 41 22 0.00% 42 22 0.00% 43 34 0.00% 44 13 0.00% 45 43 0.00% 46 28 0.00% 47 36 0.00% 48 42 0.00% 49 40 0.00% 50 45 0.00% 51 46 0.00% 52 52 0.00% 53 46 0.00% 54 66 0.00% 55 74 0.00% 56 74 0.00% 57 78 0.00% 58 91 0.00% 59 82 0.00% 60 85 0.00% 61 119 0.00% 62 158 0.00% 63 117 0.00% 64 178 0.00% 65 156 0.00% 66 188 0.00% 67 181 0.00% 68 206 0.00% 69 196 0.00% 70 266 0.00% 71 238 0.00% 72 294 0.00% 73 318 0.00% 74 361 0.00% 75 366 0.00% 76 367 0.00% 77 404 0.00% 78 405 0.00% 79 431 0.00% 80 515 0.00% 81 507 0.00% 82 556 0.00% 83 619 0.00% 84 643 0.00% 85 757 0.00% 86 765 0.00% 87 862 0.00% 88 887 0.00% 89 909 0.00% 90 1035 0.00% 91 1063 0.00% 92 1071 0.00% 93 1184 0.00% 94 1264 0.00% 95 1307 0.00% 96 1436 0.00% 97 1542 0.00% 98 1668 0.00% 99 1651 0.00% 100 1807 0.00% 101 1921 0.00% 102 1917 0.00% 103 2083 0.00% 104 2128 0.00% 105 2279 0.00% 106 2408 0.00% 107 2601 0.01% 108 2679 0.01% 109 2838 0.01% 110 2957 0.01% 111 3222 0.01% 112 3520 0.01% 113 3397 0.01% 114 3636 0.01% 115 3870 0.01% 116 4227 0.01% 117 4410 0.01% 118 4309 0.01% 119 4748 0.01% 120 5118 0.01% 121 5363 0.01% 122 5729 0.01% 123 5857 0.01% 124 6058 0.01% 125 6918 0.01% 126 6181 0.01% 127 6197 0.01% 128 6587 0.01% 129 7227 0.01% 130 7886 0.02% 131 8379 0.02% 132 8762 0.02% 133 8494 0.02% 134 8748 0.02% 135 8545 0.02% 136 9305 0.02% 137 9205 0.02% 138 9831 0.02% 139 10596 0.02% 140 10632 0.02% 141 11564 0.02% 142 12077 0.02% 143 12627 0.02% 144 12611 0.02% 145 13828 0.03% 146 16495 0.03% 147 15285 0.03% 148 16122 0.03% 149 16989 0.03% 150 51083317 99.27% 51460181 reads passed initial QC criterion=sequence-density sequence-density=0.35 sequence-density-rank=1 fanout-score=0.00 fanout-score-rank=27 prefix-density=0.00 prefix-fanout=1.0 sequence=GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTTATCCTTCCACCGTTGGAAGCGGGCAGTTGTCGCTGCTCTGTGAAGCCAGCCTCACGCTGTGCCTGCCAACATTATGGGCCGCGAAGCCTAGCTTTCGCTTAAGCTCCAACGGCCCACTACGCAACTTGGAACGGGCGGGCCATCAGTAGCACACCCAGACCAGGCCCGCAGCGCTACGGCAACGTCCACACCACCCTTAAAGCCCCCACT criterion=fanout-score sequence-density=0.01 sequence-density-rank=45 fanout-score=462.72 fanout-score-rank=1 prefix-density=2.22 prefix-fanout=1.1 sequence=CACAGGCTTCTTCGGTAAAGAGGAATCAGACGATTCAAGTGGAGTTTTTTGTAACGTATCAATAAGATAGAGCCATGCTACGGGTTGTTTCAGGCCCTAAATAAACGCGGACGCTTAAAAAATCTGTCGGACAGGCGGATTCGCATCTCTTGCAACCCACACAATCTTCGGTTCTTGGCGCGGAAGCAATTTGCTTGGCTTTACATCCATCCCAGGGTATCATTTCTAATACATCTGTTGGACAAGCTCGTACACATTGAGTGCATCCTATACATGTATCATAAATTTTTACAGAATGTGACATTGGATCTATAAATTTTTCTTTTCAACATAAAATTTTTCCGATCTGGTAAATTAGTACTGTATGAGTCATATGTATTGTAGACACCAGACGAAGCAATGGTTTGTC criterion=sequence-density sequence-density=0.29 sequence-density-rank=1 fanout-score=41.60 fanout-score-rank=15 prefix-density=11.47 prefix-fanout=1.0 sequence=GCACTGAAAATAGTCTTTACAT criterion=fanout-score sequence-density=0.09 sequence-density-rank=24 fanout-score=118.56 fanout-score-rank=1 prefix-density=11.14 prefix-fanout=1.0 sequence=CGCTTCTGCAAATGGATAACTAG ERR11006581 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 18:00:05 Started mapping on | Dec 06 18:00:06 Finished on | Dec 06 18:06:09 Mapping speed, Million of reads per hour | 510.35 Number of input reads | 51460181 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 40403910 Uniquely mapped reads % | 78.51% Average mapped length | 298.27 Number of splices: Total | 12661112 Number of splices: Annotated (sjdb) | 11819337 Number of splices: GT/AG | 12397400 Number of splices: GC/AG | 138631 Number of splices: AT/AC | 13590 Number of splices: Non-canonical | 111491 Mismatch rate per base, % | 0.37% Deletion rate per base | 0.01% Deletion average length | 1.73 Insertion rate per base | 0.01% Insertion average length | 2.15 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 9147126 % of reads mapped to multiple loci | 17.78% Number of reads mapped to too many loci | 11846 % of reads mapped to too many loci | 0.02% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.28% % of reads unmapped: other | 0.41% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1909145 1909145 1909145 N_multimapping 9147126 9147126 9147126 N_noFeature 10303020 37659974 12269728 N_ambiguous 1191938 43101 398813 UnstrandedReadsAssigned:28908952 PositiveStrandReadsAssigned:2700835 NegativeStrandReadsAssigned:27735369 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006581 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006581-trimmed-pair1.fastq ERR11006581-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 51,460,181 reads, 28,941,894 reads pseudoaligned [quant] estimated average fragment length: 274.987 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,128 rounds 52973 ERR11006581.ke.tsv 35125 ERR11006581.se.tsv 88098 total ==> ERR11006581.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 662.295 0 0 PNS24247 1044 770.013 20.3155 0.887102 PNS24249 1928 1654.01 127.265 2.58711 PNS24246 1044 770.013 20.3155 0.887102 PNS24248 1044 770.013 20.3155 0.887102 PNS24244 1471 1197.01 69.7881 1.96031 PNS24243 293 51.4949 1 0.652949 KQK14069 1603 1329.01 855.237 21.6372 KQK14071 474 202.48 8.25619 1.37101 ==> ERR11006581.se.tsv <== BRADI_1g14170v3 987 BRADI_1g53295v3 574 BRADI_1g59795v3 293 BRADI_1g07683v3 2 BRADI_1g00485v3 1 BRADI_1g20270v3 418 BRADI_1g74790v3 395 BRADI_1g09890v3 3 BRADI_1g77505v3 88 BRADI_1g48960v3 4 ERR11006581 completed mapping pipeline successfully