Starting /dee2/code/volunteer_pipeline.sh ERR11006582 current disk space = 1550565056512 free memory = 1598637196 ERR11006582 SRAfilesize 985ae068fc0dce266809eed11067717c ERR11006582.sra ERR11006582.sra file validated ERR11006582 is paired end ERR11006582 is conventional basespace ERR11006582 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006582_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.292 37.0 37.0 37.0 37.0 37.0 2 36.2485 37.0 37.0 37.0 37.0 37.0 3 36.3185 37.0 37.0 37.0 37.0 37.0 4 36.4355 37.0 37.0 37.0 37.0 37.0 5 36.387 37.0 37.0 37.0 37.0 37.0 6 36.436 37.0 37.0 37.0 37.0 37.0 7 36.33 37.0 37.0 37.0 37.0 37.0 8 36.3695 37.0 37.0 37.0 37.0 37.0 9 36.271 37.0 37.0 37.0 37.0 37.0 10-14 36.393499999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.427699999999994 37.0 37.0 37.0 37.0 37.0 20-24 36.297000000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.2787 37.0 37.0 37.0 37.0 37.0 30-34 36.2071 37.0 37.0 37.0 37.0 37.0 35-39 36.2178 37.0 37.0 37.0 37.0 37.0 40-44 36.2033 37.0 37.0 37.0 37.0 37.0 45-49 36.153800000000004 37.0 37.0 37.0 37.0 37.0 50-54 36.1659 37.0 37.0 37.0 37.0 37.0 55-59 36.13440000000001 37.0 37.0 37.0 37.0 37.0 60-64 36.0552 37.0 37.0 37.0 37.0 37.0 65-69 36.0665 37.0 37.0 37.0 37.0 37.0 70-74 36.0758 37.0 37.0 37.0 37.0 37.0 75-79 35.9822 37.0 37.0 37.0 37.0 37.0 80-84 35.962 37.0 37.0 37.0 37.0 37.0 85-89 35.950199999999995 37.0 37.0 37.0 37.0 37.0 90-94 35.91709999999999 37.0 37.0 37.0 37.0 37.0 95-99 35.945299999999996 37.0 37.0 37.0 37.0 37.0 100-104 35.9484 37.0 37.0 37.0 37.0 37.0 105-109 35.7597 37.0 37.0 37.0 37.0 37.0 110-114 35.7382 37.0 37.0 37.0 37.0 37.0 115-119 35.715999999999994 37.0 37.0 37.0 37.0 37.0 120-124 35.7384 37.0 37.0 37.0 37.0 37.0 125-129 35.5938 37.0 37.0 37.0 37.0 37.0 130-134 35.501099999999994 37.0 37.0 37.0 37.0 37.0 135-139 35.4105 37.0 37.0 37.0 37.0 37.0 140-144 35.25129999999999 37.0 37.0 37.0 32.2 37.0 145-149 35.376400000000004 37.0 37.0 37.0 34.6 37.0 150 35.493 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 1.0 23 5.0 24 3.0 25 4.0 26 6.0 27 18.0 28 32.0 29 35.0 30 57.0 31 53.0 32 87.0 33 101.0 34 146.0 35 325.0 36 2844.0 37 282.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.875 13.825000000000001 16.175 33.125 2 29.4 10.674999999999999 26.8 33.125 3 21.55 13.100000000000001 28.499999999999996 36.85 4 26.424999999999997 15.7 25.1 32.775 5 29.25 19.75 25.224999999999998 25.775 6 24.825 29.475 19.7 26.0 7 17.875 29.95 33.650000000000006 18.525 8 16.85 30.325000000000003 33.25 19.575 9 16.675 27.675 36.225 19.425 10-14 19.845 32.18 26.705000000000002 21.27 15-19 19.919999999999998 34.675 24.505 20.9 20-24 19.400000000000002 29.2 28.115000000000002 23.285 25-29 21.98 31.785000000000004 25.15 21.085 30-34 23.93 31.03 24.759999999999998 20.28 35-39 22.55 31.369999999999997 25.055 21.025 40-44 19.5 31.635 25.505 23.36 45-49 20.27 29.395 27.55 22.785 50-54 20.68 33.31 25.045 20.965 55-59 22.05 30.12 22.994999999999997 24.834999999999997 60-64 20.105 31.895 25.009999999999998 22.99 65-69 20.599999999999998 30.2 25.124999999999996 24.075 70-74 23.11 30.064999999999998 21.78 25.045 75-79 22.305 30.85 24.595 22.25 80-84 22.88 29.285 24.935 22.900000000000002 85-89 23.974999999999998 29.395 24.86 21.77 90-94 21.3 30.04 26.26 22.400000000000002 95-99 24.795 29.17 23.61 22.425 100-104 21.66 30.575000000000003 24.38 23.385 105-109 21.825 29.020000000000003 25.72 23.435 110-114 23.380000000000003 27.650000000000002 25.915 23.055 115-119 19.86 31.095 25.82 23.225 120-124 19.905 30.764999999999997 24.73 24.6 125-129 20.23 31.8 22.615 25.355 130-134 23.05 30.464999999999996 24.44 22.045 135-139 23.64 29.525000000000002 23.189999999999998 23.645 140-144 24.605 29.235 25.635 20.525 145-149 23.14 30.45 24.560000000000002 21.85 150 23.400000000000002 26.825 27.6 22.175 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 1.5 21 1.5 22 2.0 23 2.5 24 2.5 25 4.0 26 5.0 27 6.5 28 11.0 29 17.0 30 20.5 31 23.0 32 23.5 33 27.0 34 44.0 35 60.5 36 88.5 37 173.5 38 301.0 39 291.0 40 255.0 41 303.0 42 267.5 43 231.5 44 222.5 45 232.5 46 215.0 47 156.5 48 112.0 49 74.5 50 64.0 51 53.0 52 40.0 53 32.5 54 32.0 55 30.5 56 26.0 57 26.5 58 24.0 59 22.5 60 25.0 61 27.0 62 29.0 63 31.5 64 62.5 65 77.0 66 40.0 67 22.0 68 21.5 69 17.0 70 18.0 71 13.0 72 10.0 73 11.5 74 12.0 75 10.5 76 8.0 77 10.5 78 7.5 79 5.0 80 6.0 81 2.0 82 1.0 83 2.0 84 1.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.324999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 78.57438872772482 47.4 2 13.302942395358475 16.05 3 3.398259428097804 6.15 4 1.0360547036883547 2.5 5 0.7045171985080813 2.125 6 0.3729796933278077 1.35 7 0.20721094073767096 0.8750000000000001 8 0.5801906340654787 2.8000000000000003 9 0.16576875259013676 0.8999999999999999 >10 1.6162453377538335 16.45 >50 0.0 0.0 >100 0.04144218814753419 3.4000000000000004 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 136 3.4000000000000004 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 41 1.0250000000000001 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 39 0.975 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 38 0.95 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 37 0.9249999999999999 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 32 0.8 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 30 0.75 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 29 0.7250000000000001 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 27 0.675 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 19 0.475 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 17 0.42500000000000004 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 17 0.42500000000000004 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 16 0.4 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 15 0.375 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 15 0.375 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 14 0.35000000000000003 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 13 0.325 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 13 0.325 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 13 0.325 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 13 0.325 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 13 0.325 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 13 0.325 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 13 0.325 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 13 0.325 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 12 0.3 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 12 0.3 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 12 0.3 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 11 0.27499999999999997 No Hit CACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCG 11 0.27499999999999997 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 10 0.25 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 10 0.25 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 10 0.25 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 10 0.25 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 10 0.25 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 10 0.25 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 10 0.25 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 10 0.25 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 10 0.25 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 10 0.25 No Hit TAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGA 10 0.25 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 9 0.22499999999999998 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 9 0.22499999999999998 No Hit ACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 9 0.22499999999999998 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 9 0.22499999999999998 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 8 0.2 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 8 0.2 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 8 0.2 No Hit TGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATA 8 0.2 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 8 0.2 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 8 0.2 No Hit GGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAG 8 0.2 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 8 0.2 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 8 0.2 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 8 0.2 No Hit CTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 8 0.2 No Hit GGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGG 8 0.2 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 8 0.2 No Hit AGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGG 8 0.2 No Hit TGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 7 0.17500000000000002 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 7 0.17500000000000002 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 7 0.17500000000000002 No Hit ATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAG 7 0.17500000000000002 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 7 0.17500000000000002 No Hit TCTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTA 6 0.15 No Hit GGGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACA 6 0.15 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 6 0.15 No Hit GCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCC 6 0.15 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 6 0.15 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 6 0.15 No Hit TGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCT 6 0.15 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 6 0.15 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 6 0.15 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 5 0.125 No Hit CCCTGGCCCGACTCCCCAACCTAGGGTAGCTAAGTGTGGAAGTAAAATCA 5 0.125 No Hit GACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGG 5 0.125 No Hit GGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 5 0.125 No Hit GAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATG 5 0.125 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 5 0.125 No Hit GCTGCTGCACCAGGTGCATTTCCCCAAGGATGTCCTAAAGTTCCTCCACC 5 0.125 No Hit TGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTG 5 0.125 No Hit GGCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGAT 5 0.125 No Hit AGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 5 0.125 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 5 0.125 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 5 0.125 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 5 0.125 No Hit GTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCC 5 0.125 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 5 0.125 No Hit GAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATG 5 0.125 No Hit CGCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0125 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.025 0.0 0.0 0.0 0.0 92-93 0.025 0.0 0.0 0.0 0.0 94-95 0.025 0.0 0.0 0.0 0.0 96-97 0.037500000000000006 0.0 0.0 0.0 0.0 98-99 0.0625 0.0 0.0 0.0 0.0 100-101 0.075 0.0 0.0 0.0 0.0 102-103 0.075 0.0 0.0 0.0 0.0 104-105 0.075 0.0 0.0 0.0 0.0 106-107 0.075 0.0 0.0 0.0 0.0 108-109 0.075 0.0 0.0 0.0 0.0 110-111 0.16249999999999998 0.0 0.0 0.0 0.0 112-113 0.175 0.0 0.0 0.0 0.0 114-115 0.1875 0.0 0.0 0.0 0.0 116-117 0.2 0.0 0.0 0.0 0.0 118-119 0.2 0.0 0.0 0.0 0.0 120-121 0.2 0.0 0.0 0.0 0.0 122-123 0.21250000000000002 0.0 0.0 0.0 0.0 124-125 0.2375 0.0 0.0 0.0 0.0 126-127 0.3 0.0 0.0 0.0 0.0 128-129 0.3 0.0 0.0 0.0 0.0 130-131 0.3125 0.0 0.0 0.0 0.0 132-133 0.325 0.0 0.0 0.0 0.0 134-135 0.35 0.0 0.0 0.0 0.0 136-137 0.3625 0.0 0.0 0.0 0.0 138 0.4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTTCT 20 3.687869E-4 108.0 1 TCTTTTC 35 3.1411873E-5 82.28571 5 TTCTTTT 30 0.0018473949 72.0 4 TTTCTTT 35 0.0034045284 61.714283 3 CTTTCTT 35 0.0034045284 61.714283 2 TTCTTCA 45 0.009205684 48.0 9 TATGTTA 35 0.0036813593 20.571428 8 CTTCAAA 40 0.007966741 18.0 4 AAAATTC 40 0.007966741 18.0 9 >>END_MODULE ERR11006582 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006582_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.582 37.0 37.0 37.0 37.0 37.0 2 36.2435 37.0 37.0 37.0 37.0 37.0 3 36.2865 37.0 37.0 37.0 37.0 37.0 4 36.2045 37.0 37.0 37.0 37.0 37.0 5 36.279 37.0 37.0 37.0 37.0 37.0 6 36.302 37.0 37.0 37.0 37.0 37.0 7 36.2625 37.0 37.0 37.0 37.0 37.0 8 36.331 37.0 37.0 37.0 37.0 37.0 9 36.2375 37.0 37.0 37.0 37.0 37.0 10-14 36.299899999999994 37.0 37.0 37.0 37.0 37.0 15-19 36.264300000000006 37.0 37.0 37.0 37.0 37.0 20-24 36.270799999999994 37.0 37.0 37.0 37.0 37.0 25-29 36.2112 37.0 37.0 37.0 37.0 37.0 30-34 36.1706 37.0 37.0 37.0 37.0 37.0 35-39 36.1387 37.0 37.0 37.0 37.0 37.0 40-44 36.1477 37.0 37.0 37.0 37.0 37.0 45-49 36.1397 37.0 37.0 37.0 37.0 37.0 50-54 36.076499999999996 37.0 37.0 37.0 37.0 37.0 55-59 35.9923 37.0 37.0 37.0 37.0 37.0 60-64 35.948899999999995 37.0 37.0 37.0 37.0 37.0 65-69 35.9529 37.0 37.0 37.0 37.0 37.0 70-74 35.889 37.0 37.0 37.0 37.0 37.0 75-79 35.8699 37.0 37.0 37.0 37.0 37.0 80-84 35.80400000000001 37.0 37.0 37.0 37.0 37.0 85-89 35.7385 37.0 37.0 37.0 37.0 37.0 90-94 35.787 37.0 37.0 37.0 37.0 37.0 95-99 35.604 37.0 37.0 37.0 37.0 37.0 100-104 35.5729 37.0 37.0 37.0 37.0 37.0 105-109 35.5343 37.0 37.0 37.0 37.0 37.0 110-114 35.481100000000005 37.0 37.0 37.0 37.0 37.0 115-119 35.3513 37.0 37.0 37.0 34.6 37.0 120-124 35.220299999999995 37.0 37.0 37.0 29.8 37.0 125-129 35.3375 37.0 37.0 37.0 37.0 37.0 130-134 35.1168 37.0 37.0 37.0 27.4 37.0 135-139 35.139599999999994 37.0 37.0 37.0 25.0 37.0 140-144 34.990899999999996 37.0 37.0 37.0 25.0 37.0 145-149 35.0695 37.0 37.0 37.0 25.0 37.0 150 34.6385 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 0.0 15 0.0 16 1.0 17 2.0 18 1.0 19 1.0 20 1.0 21 1.0 22 4.0 23 4.0 24 10.0 25 11.0 26 15.0 27 27.0 28 18.0 29 30.0 30 33.0 31 51.0 32 74.0 33 109.0 34 179.0 35 575.0 36 2665.0 37 187.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.933673469387756 25.66326530612245 19.158163265306122 27.24489795918367 2 25.2 24.775 33.5 16.525000000000002 3 19.6 27.3 33.2 19.900000000000002 4 24.474999999999998 27.224999999999998 28.925 19.375 5 24.075 27.400000000000002 30.675 17.849999999999998 6 20.925 30.099999999999998 29.225 19.75 7 20.424999999999997 19.775000000000002 40.75 19.05 8 22.075 22.400000000000002 31.35 24.175 9 22.525000000000002 20.65 35.699999999999996 21.125 10-14 23.855 26.155 29.294999999999998 20.695 15-19 23.855 25.019999999999996 30.435000000000002 20.69 20-24 24.795 23.205000000000002 31.8 20.200000000000003 25-29 24.65 23.765 30.53 21.055 30-34 24.87 24.075 30.04 21.015 35-39 23.87 24.37 30.080000000000002 21.68 40-44 22.505 25.39 30.53 21.575 45-49 22.695 26.875 29.160000000000004 21.27 50-54 22.525000000000002 26.025 29.015 22.435 55-59 22.425 25.4 29.310000000000002 22.865 60-64 22.48 24.62 30.3 22.6 65-69 23.025000000000002 24.490000000000002 29.835 22.650000000000002 70-74 23.345 24.9 30.5 21.255 75-79 23.830000000000002 25.045 30.37 20.755000000000003 80-84 24.83 25.205 29.32 20.645 85-89 24.27 25.014999999999997 28.17 22.545 90-94 23.52 25.130000000000003 29.145 22.205 95-99 23.44 24.740000000000002 29.225 22.595000000000002 100-104 24.21 23.665 30.735 21.39 105-109 25.945 22.645 29.93 21.48 110-114 23.72 23.51 30.44 22.33 115-119 23.400000000000002 25.064999999999998 30.055 21.48 120-124 24.154999999999998 24.845 28.57 22.43 125-129 22.73 24.97 30.195 22.105 130-134 22.615 26.200000000000003 28.865000000000002 22.32 135-139 23.11 25.380000000000003 30.325000000000003 21.185000000000002 140-144 22.994999999999997 25.615 30.56 20.830000000000002 145-149 21.97 24.615000000000002 31.785000000000004 21.63 150 23.375 22.775000000000002 29.549999999999997 24.3 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0 17 1.0 18 0.0 19 1.0 20 1.0 21 0.5 22 0.5 23 2.5 24 5.0 25 8.0 26 14.0 27 16.5 28 17.0 29 21.5 30 24.5 31 23.5 32 24.5 33 34.5 34 58.5 35 85.0 36 128.0 37 223.0 38 253.5 39 217.5 40 233.0 41 253.5 42 229.0 43 235.0 44 269.5 45 230.5 46 174.0 47 123.0 48 108.0 49 92.0 50 54.5 51 50.5 52 43.5 53 39.5 54 35.0 55 22.0 56 18.0 57 28.0 58 31.0 59 26.5 60 30.0 61 31.0 62 27.5 63 51.0 64 60.5 65 49.5 66 38.0 67 24.0 68 30.5 69 32.0 70 22.5 71 20.5 72 22.5 73 22.0 74 16.5 75 12.5 76 10.0 77 4.0 78 5.5 79 9.5 80 8.0 81 5.0 82 3.0 83 3.0 84 2.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.1 #Duplication Level Percentage of deduplicated Percentage of total 1 76.4820592823713 49.025 2 13.72854914196568 17.599999999999998 3 3.9001560062402496 7.5 4 1.9110764430577223 4.9 5 1.5600624024960998 5.0 6 0.858034321372855 3.3000000000000003 7 0.27301092043681746 1.225 8 0.3510140405616225 1.7999999999999998 9 0.19500780031201248 1.125 >10 0.702028081123245 6.7250000000000005 >50 0.0390015600624025 1.7999999999999998 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 72 1.7999999999999998 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 24 0.6 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 24 0.6 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 23 0.575 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 21 0.525 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 17 0.42500000000000004 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 17 0.42500000000000004 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 14 0.35000000000000003 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 14 0.35000000000000003 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 14 0.35000000000000003 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 13 0.325 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 12 0.3 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 12 0.3 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 12 0.3 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 11 0.27499999999999997 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 11 0.27499999999999997 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 10 0.25 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 10 0.25 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 10 0.25 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 9 0.22499999999999998 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 9 0.22499999999999998 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 9 0.22499999999999998 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 9 0.22499999999999998 No Hit CTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTC 9 0.22499999999999998 No Hit TGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGA 8 0.2 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 8 0.2 No Hit TGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCAT 8 0.2 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 8 0.2 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 8 0.2 No Hit GTAGCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGT 8 0.2 No Hit ATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTT 8 0.2 No Hit GAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAAT 8 0.2 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 8 0.2 No Hit GATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTC 7 0.17500000000000002 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 7 0.17500000000000002 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 7 0.17500000000000002 No Hit GCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAG 7 0.17500000000000002 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 7 0.17500000000000002 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 7 0.17500000000000002 No Hit GAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGT 7 0.17500000000000002 No Hit AAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATC 6 0.15 No Hit GCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCGACTG 6 0.15 No Hit CTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTC 6 0.15 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 6 0.15 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 6 0.15 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 6 0.15 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 6 0.15 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 6 0.15 No Hit CAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCT 6 0.15 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 6 0.15 No Hit CTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTTATGA 6 0.15 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 6 0.15 No Hit TGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTC 6 0.15 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 6 0.15 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 6 0.15 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 6 0.15 No Hit TCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCA 6 0.15 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 6 0.15 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 6 0.15 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 6 0.15 No Hit ATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTG 6 0.15 No Hit AGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGC 6 0.15 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 5 0.125 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 5 0.125 No Hit GTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTC 5 0.125 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 5 0.125 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 5 0.125 No Hit GTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTAT 5 0.125 No Hit TATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATA 5 0.125 No Hit GAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAAT 5 0.125 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 5 0.125 No Hit CGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGAC 5 0.125 No Hit GCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTC 5 0.125 No Hit AATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTAT 5 0.125 No Hit ATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATAT 5 0.125 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 5 0.125 No Hit CTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCTACTTCT 5 0.125 No Hit TATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACC 5 0.125 No Hit CCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGA 5 0.125 No Hit AGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGT 5 0.125 No Hit TGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCT 5 0.125 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 5 0.125 No Hit GCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGT 5 0.125 No Hit GAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCG 5 0.125 No Hit TAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAG 5 0.125 No Hit TGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCT 5 0.125 No Hit ATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAG 5 0.125 No Hit AGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACT 5 0.125 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 5 0.125 No Hit CTATTGGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGT 5 0.125 No Hit GCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGC 5 0.125 No Hit CGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTG 5 0.125 No Hit CACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTAT 5 0.125 No Hit CGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATG 5 0.125 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 5 0.125 No Hit CAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTC 5 0.125 No Hit TAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACA 5 0.125 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 5 0.125 No Hit AGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTC 5 0.125 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 5 0.125 No Hit CTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTC 5 0.125 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0125 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.025 0.0 0.0 0.0 0.0 92-93 0.025 0.0 0.0 0.0 0.0 94-95 0.025 0.0 0.0 0.0 0.0 96-97 0.037500000000000006 0.0 0.0 0.0 0.0 98-99 0.0625 0.0 0.0 0.0 0.0 100-101 0.075 0.0 0.0 0.0 0.0 102-103 0.075 0.0 0.0 0.0 0.0 104-105 0.075 0.0 0.0 0.0 0.0 106-107 0.075 0.0 0.0 0.0 0.0 108-109 0.075 0.0 0.0 0.0 0.0 110-111 0.16249999999999998 0.0 0.0 0.0 0.0 112-113 0.175 0.0 0.0 0.0 0.0 114-115 0.1875 0.0 0.0 0.0 0.0 116-117 0.2 0.0 0.0 0.0 0.0 118-119 0.2 0.0 0.0 0.0 0.0 120-121 0.2 0.0 0.0 0.0 0.0 122-123 0.21250000000000002 0.0 0.0 0.0 0.0 124-125 0.2375 0.0 0.0 0.0 0.0 126-127 0.3 0.0 0.0 0.0 0.0 128-129 0.3 0.0 0.0 0.0 0.0 130-131 0.3125 0.0 0.0 0.0 0.0 132-133 0.325 0.0 0.0 0.0 0.0 134-135 0.35 0.0 0.0 0.0 0.0 136-137 0.3625 0.0 0.0 0.0 0.0 138 0.4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 2534341 spots for ERR11006582.sra Written 2534341 spots for ERR11006582.sra Read 2534341 spots for ERR11006582.sra Written 2534341 spots for ERR11006582.sra Read 2534341 spots for ERR11006582.sra Written 2534341 spots for ERR11006582.sra Read 2534341 spots for ERR11006582.sra Written 2534341 spots for ERR11006582.sra Read 2534341 spots for ERR11006582.sra Written 2534341 spots for ERR11006582.sra Read 2534341 spots for ERR11006582.sra Written 2534341 spots for ERR11006582.sra Read 2534341 spots for ERR11006582.sra Written 2534341 spots for ERR11006582.sra Read 2534341 spots for ERR11006582.sra Written 2534341 spots for ERR11006582.sra Read 2534341 spots for ERR11006582.sra Written 2534341 spots for ERR11006582.sra Read 2534341 spots for ERR11006582.sra Written 2534341 spots for ERR11006582.sra Read 2534341 spots for ERR11006582.sra Written 2534341 spots for ERR11006582.sra Read 2534341 spots for ERR11006582.sra Written 2534341 spots for ERR11006582.sra Read 2534341 spots for ERR11006582.sra Written 2534341 spots for ERR11006582.sra Read 2534351 spots for ERR11006582.sra Written 2534351 spots for ERR11006582.sra Read 2534341 spots for ERR11006582.sra Written 2534341 spots for ERR11006582.sra Read 2534341 spots for ERR11006582.sra Written 2534341 spots for ERR11006582.sra Read 2534341 spots for ERR11006582.sra Written 2534341 spots for ERR11006582.sra Read 2534341 spots for ERR11006582.sra Written 2534341 spots for ERR11006582.sra Read 2534341 spots for ERR11006582.sra Written 2534341 spots for ERR11006582.sra Read 2534341 spots for ERR11006582.sra Written 2534341 spots for ERR11006582.sra SRR ids: ['ERR11006582.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_gdl0qm91 ERR11006582.sra spots: 50686830 blocks: [[1, 2534341], [2534342, 5068682], [5068683, 7603023], [7603024, 10137364], [10137365, 12671705], [12671706, 15206046], [15206047, 17740387], [17740388, 20274728], [20274729, 22809069], [22809070, 25343410], [25343411, 27877751], [27877752, 30412092], [30412093, 32946433], [32946434, 35480774], [35480775, 38015115], [38015116, 40549456], [40549457, 43083797], [43083798, 45618138], [45618139, 48152479], [48152480, 50686830]] ERR11006582 file size 18623791 ERR11006582 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006582 ERR11006582_1.fastq ERR11006582_2.fastq Input file: ERR11006582_1.fastq Paired file: ERR11006582_2.fastq trimmed: ERR11006582-trimmed-pair1.fastq, ERR11006582-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 18:00:26 2024 >> started Fri Dec 6 18:01:29 2024 >> done (62.767s) 50686830 read pairs processed; of these: 107 ( 0.00%) short read pairs filtered out after trimming by size control 557 ( 0.00%) empty read pairs filtered out after trimming by size control 50686166 (100.00%) read pairs available; of these: 386013 ( 0.76%) trimmed read pairs available after processing 50300153 (99.24%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 7 0.00% 20 5 0.00% 21 10 0.00% 22 20 0.00% 23 4 0.00% 24 2 0.00% 25 38 0.00% 26 12 0.00% 27 10 0.00% 28 16 0.00% 29 27 0.00% 30 21 0.00% 31 53 0.00% 32 7 0.00% 33 13 0.00% 34 9 0.00% 35 14 0.00% 36 15 0.00% 37 19 0.00% 38 27 0.00% 39 13 0.00% 40 30 0.00% 41 21 0.00% 42 20 0.00% 43 25 0.00% 44 25 0.00% 45 20 0.00% 46 32 0.00% 47 32 0.00% 48 26 0.00% 49 38 0.00% 50 45 0.00% 51 52 0.00% 52 53 0.00% 53 66 0.00% 54 77 0.00% 55 59 0.00% 56 82 0.00% 57 86 0.00% 58 87 0.00% 59 79 0.00% 60 97 0.00% 61 108 0.00% 62 125 0.00% 63 115 0.00% 64 137 0.00% 65 179 0.00% 66 190 0.00% 67 159 0.00% 68 178 0.00% 69 211 0.00% 70 192 0.00% 71 207 0.00% 72 274 0.00% 73 297 0.00% 74 337 0.00% 75 407 0.00% 76 367 0.00% 77 397 0.00% 78 380 0.00% 79 442 0.00% 80 428 0.00% 81 463 0.00% 82 551 0.00% 83 612 0.00% 84 663 0.00% 85 765 0.00% 86 771 0.00% 87 918 0.00% 88 948 0.00% 89 904 0.00% 90 1044 0.00% 91 1002 0.00% 92 1099 0.00% 93 1138 0.00% 94 1232 0.00% 95 1288 0.00% 96 1339 0.00% 97 1464 0.00% 98 1637 0.00% 99 1666 0.00% 100 1819 0.00% 101 1819 0.00% 102 1969 0.00% 103 2084 0.00% 104 2088 0.00% 105 2295 0.00% 106 2552 0.01% 107 2546 0.01% 108 2725 0.01% 109 2871 0.01% 110 3115 0.01% 111 3112 0.01% 112 3559 0.01% 113 3633 0.01% 114 3651 0.01% 115 3719 0.01% 116 4191 0.01% 117 4326 0.01% 118 4506 0.01% 119 4726 0.01% 120 5304 0.01% 121 5613 0.01% 122 6038 0.01% 123 6087 0.01% 124 6186 0.01% 125 7291 0.01% 126 6472 0.01% 127 6419 0.01% 128 6974 0.01% 129 7606 0.02% 130 8129 0.02% 131 8534 0.02% 132 9085 0.02% 133 8687 0.02% 134 9071 0.02% 135 8614 0.02% 136 9414 0.02% 137 9600 0.02% 138 10085 0.02% 139 10603 0.02% 140 10970 0.02% 141 11939 0.02% 142 12179 0.02% 143 13146 0.03% 144 13135 0.03% 145 14340 0.03% 146 17370 0.03% 147 15710 0.03% 148 16696 0.03% 149 17382 0.03% 150 50300153 99.24% 50686166 reads passed initial QC criterion=sequence-density sequence-density=0.31 sequence-density-rank=1 fanout-score=0.00 fanout-score-rank=28 prefix-density=0.00 prefix-fanout=1.0 sequence=TGCGGGAACTTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=40 fanout-score=30.77 fanout-score-rank=1 prefix-density=0.17 prefix-fanout=1.1 sequence=TGGTAGAACAAGATATTGGGTATTTCTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTACCAAGGAACCATGCATAGCACTGAATAGGGAAC criterion=sequence-density sequence-density=0.22 sequence-density-rank=1 fanout-score=14.44 fanout-score-rank=12 prefix-density=3.15 prefix-fanout=1.0 sequence=TTGCGTAGTGGATCTGCTGGGGCCTATGCGAAAGCTGGGCCTCACGAATTCTATAGTGGCAGGCACCGCGTTAGGCTGGCTTC criterion=fanout-score sequence-density=0.08 sequence-density-rank=19 fanout-score=161.26 fanout-score-rank=1 prefix-density=11.96 prefix-fanout=1.0 sequence=ATAACTAGCACAGAAAATCGTCT ERR11006582 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 18:02:07 Started mapping on | Dec 06 18:02:08 Finished on | Dec 06 18:07:54 Mapping speed, Million of reads per hour | 527.37 Number of input reads | 50686166 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 39097211 Uniquely mapped reads % | 77.14% Average mapped length | 298.47 Number of splices: Total | 11283577 Number of splices: Annotated (sjdb) | 10502978 Number of splices: GT/AG | 11017326 Number of splices: GC/AG | 135478 Number of splices: AT/AC | 12524 Number of splices: Non-canonical | 118249 Mismatch rate per base, % | 0.30% Deletion rate per base | 0.01% Deletion average length | 1.66 Insertion rate per base | 0.01% Insertion average length | 2.15 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 10102902 % of reads mapped to multiple loci | 19.93% Number of reads mapped to too many loci | 9402 % of reads mapped to too many loci | 0.02% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.65% % of reads unmapped: other | 0.26% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1486053 1486053 1486053 N_multimapping 10102902 10102902 10102902 N_noFeature 10680002 36319719 12557889 N_ambiguous 1406692 46946 496764 UnstrandedReadsAssigned:27010517 PositiveStrandReadsAssigned:2730546 NegativeStrandReadsAssigned:26042558 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006582 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006582-trimmed-pair1.fastq ERR11006582-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 50,686,166 reads, 28,057,756 reads pseudoaligned [quant] estimated average fragment length: 276.082 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,232 rounds 52973 ERR11006582.ke.tsv 35125 ERR11006582.se.tsv 88098 total ==> ERR11006582.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 661.302 0 0 PNS24247 1044 768.918 22.8626 0.989948 PNS24249 1928 1652.92 141.467 2.8495 PNS24246 1044 768.918 22.8626 0.989948 PNS24248 1044 768.918 22.8626 0.989948 PNS24244 1471 1195.92 47.9454 1.33479 PNS24243 293 51.7738 1 0.643066 KQK14069 1603 1327.92 826.078 20.7117 KQK14071 474 201.549 6.08749 1.0056 ==> ERR11006582.se.tsv <== BRADI_1g14170v3 927 BRADI_1g53295v3 133 BRADI_1g59795v3 323 BRADI_1g07683v3 0 BRADI_1g00485v3 2 BRADI_1g20270v3 310 BRADI_1g74790v3 298 BRADI_1g09890v3 7 BRADI_1g77505v3 131 BRADI_1g48960v3 5 ERR11006582 completed mapping pipeline successfully