Starting /dee2/code/volunteer_pipeline.sh ERR11006583 current disk space = 1550693552128 free memory = 1369621816 ERR11006583 SRAfilesize dd7f77de89935c28a2cc638503ebde1a ERR11006583.sra ERR11006583.sra file validated ERR11006583 is paired end ERR11006583 is conventional basespace ERR11006583 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006583_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.3745 37.0 37.0 37.0 37.0 37.0 2 36.3065 37.0 37.0 37.0 37.0 37.0 3 36.2875 37.0 37.0 37.0 37.0 37.0 4 36.529 37.0 37.0 37.0 37.0 37.0 5 36.321 37.0 37.0 37.0 37.0 37.0 6 36.452 37.0 37.0 37.0 37.0 37.0 7 36.302 37.0 37.0 37.0 37.0 37.0 8 36.34 37.0 37.0 37.0 37.0 37.0 9 36.3015 37.0 37.0 37.0 37.0 37.0 10-14 36.424099999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.426 37.0 37.0 37.0 37.0 37.0 20-24 36.3786 37.0 37.0 37.0 37.0 37.0 25-29 36.2315 37.0 37.0 37.0 37.0 37.0 30-34 36.3163 37.0 37.0 37.0 37.0 37.0 35-39 36.213 37.0 37.0 37.0 37.0 37.0 40-44 36.2345 37.0 37.0 37.0 37.0 37.0 45-49 36.2115 37.0 37.0 37.0 37.0 37.0 50-54 36.17209999999999 37.0 37.0 37.0 37.0 37.0 55-59 36.154900000000005 37.0 37.0 37.0 37.0 37.0 60-64 36.1203 37.0 37.0 37.0 37.0 37.0 65-69 36.1244 37.0 37.0 37.0 37.0 37.0 70-74 36.0604 37.0 37.0 37.0 37.0 37.0 75-79 36.02310000000001 37.0 37.0 37.0 37.0 37.0 80-84 35.9987 37.0 37.0 37.0 37.0 37.0 85-89 35.9266 37.0 37.0 37.0 37.0 37.0 90-94 35.898 37.0 37.0 37.0 37.0 37.0 95-99 35.9964 37.0 37.0 37.0 37.0 37.0 100-104 35.8661 37.0 37.0 37.0 37.0 37.0 105-109 35.7899 37.0 37.0 37.0 37.0 37.0 110-114 35.66669999999999 37.0 37.0 37.0 37.0 37.0 115-119 35.7044 37.0 37.0 37.0 37.0 37.0 120-124 35.6888 37.0 37.0 37.0 37.0 37.0 125-129 35.63459999999999 37.0 37.0 37.0 37.0 37.0 130-134 35.4864 37.0 37.0 37.0 37.0 37.0 135-139 35.43050000000001 37.0 37.0 37.0 37.0 37.0 140-144 35.2228 37.0 37.0 37.0 32.2 37.0 145-149 35.35940000000001 37.0 37.0 37.0 34.6 37.0 150 35.2605 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 3.0 23 1.0 24 3.0 25 4.0 26 7.0 27 15.0 28 26.0 29 29.0 30 55.0 31 68.0 32 96.0 33 111.0 34 160.0 35 291.0 36 2805.0 37 325.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.775 13.850000000000001 15.35 35.025 2 28.925 11.525 27.425 32.125 3 23.65 13.575000000000001 27.55 35.225 4 26.85 17.7 25.6 29.849999999999998 5 28.050000000000004 18.875 27.224999999999998 25.85 6 25.3 29.75 18.8 26.150000000000002 7 15.975 31.525 34.0 18.5 8 17.75 28.749999999999996 32.9 20.599999999999998 9 17.1 26.950000000000003 35.125 20.825 10-14 20.555 32.225 26.405 20.815 15-19 20.51 34.31 23.61 21.57 20-24 19.985 28.754999999999995 27.794999999999998 23.465 25-29 22.42 32.25 24.815 20.515 30-34 22.96 31.259999999999998 24.474999999999998 21.305 35-39 23.515 31.6 24.38 20.505000000000003 40-44 20.535 30.61 24.995 23.86 45-49 20.575 29.325000000000003 27.169999999999998 22.93 50-54 21.505 32.05 25.5 20.945 55-59 22.49 30.23 22.97 24.310000000000002 60-64 20.105 30.94 25.445 23.51 65-69 21.125 30.275000000000002 24.635 23.965 70-74 23.505000000000003 29.970000000000002 22.02 24.505 75-79 22.945 30.235 24.205 22.615 80-84 22.495 29.054999999999996 25.145 23.305 85-89 24.585 29.515 24.48 21.42 90-94 22.025 29.659999999999997 25.485000000000003 22.830000000000002 95-99 24.51 29.2 22.915 23.375 100-104 21.505 30.154999999999998 24.555 23.785 105-109 22.105 28.595 25.615 23.685000000000002 110-114 23.75 28.275 24.415 23.56 115-119 20.29 30.845 25.4 23.465 120-124 19.72 30.635 24.3 25.345000000000002 125-129 21.255 32.07 21.45 25.224999999999998 130-134 23.369999999999997 30.37 23.880000000000003 22.38 135-139 24.04 29.825000000000003 22.465 23.669999999999998 140-144 24.495 29.64 25.385 20.48 145-149 23.3 30.44 23.810000000000002 22.45 150 24.175 27.05 27.200000000000003 21.575 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 2.0 21 1.5 22 2.0 23 3.5 24 2.5 25 2.0 26 3.0 27 5.0 28 10.5 29 17.0 30 17.5 31 17.5 32 25.5 33 36.5 34 41.5 35 49.5 36 78.0 37 164.0 38 295.0 39 290.0 40 248.5 41 291.5 42 263.5 43 233.5 44 232.0 45 221.0 46 177.0 47 124.5 48 120.0 49 112.5 50 84.0 51 53.0 52 46.0 53 46.5 54 37.0 55 29.5 56 34.5 57 32.5 58 25.0 59 29.0 60 23.5 61 21.0 62 26.0 63 32.5 64 56.0 65 73.0 66 51.0 67 25.0 68 22.5 69 25.0 70 24.0 71 20.5 72 19.0 73 15.5 74 10.0 75 7.5 76 7.5 77 6.0 78 6.5 79 7.5 80 5.5 81 3.5 82 2.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.675000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 79.85865724381625 50.849999999999994 2 12.21044365920691 15.55 3 3.455045151158225 6.6000000000000005 4 1.2956419316843346 3.3000000000000003 5 0.7852375343541421 2.5 6 0.5104043973301924 1.95 7 0.3140950137416569 1.4000000000000001 8 0.23557126030624262 1.2 9 0.23557126030624262 1.35 >10 1.0600706713780919 11.799999999999999 >50 0.0 0.0 >100 0.03926187671770711 3.5000000000000004 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 140 3.5000000000000004 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 43 1.075 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 37 0.9249999999999999 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 34 0.8500000000000001 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 27 0.675 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 24 0.6 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 22 0.5499999999999999 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 19 0.475 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 19 0.475 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 17 0.42500000000000004 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 17 0.42500000000000004 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 16 0.4 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 16 0.4 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 16 0.4 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 15 0.375 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 15 0.375 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 13 0.325 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 12 0.3 No Hit TGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATA 12 0.3 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 12 0.3 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 11 0.27499999999999997 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 11 0.27499999999999997 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 11 0.27499999999999997 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 11 0.27499999999999997 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 11 0.27499999999999997 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 11 0.27499999999999997 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 10 0.25 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 10 0.25 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 9 0.22499999999999998 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 9 0.22499999999999998 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 9 0.22499999999999998 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 9 0.22499999999999998 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 9 0.22499999999999998 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 9 0.22499999999999998 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 8 0.2 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 8 0.2 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 8 0.2 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 8 0.2 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 8 0.2 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 8 0.2 No Hit CCGCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGAT 7 0.17500000000000002 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 7 0.17500000000000002 No Hit AGGGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAAC 7 0.17500000000000002 No Hit CTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAG 7 0.17500000000000002 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 7 0.17500000000000002 No Hit TCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGT 7 0.17500000000000002 No Hit TCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGT 7 0.17500000000000002 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 7 0.17500000000000002 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 6 0.15 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 6 0.15 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 6 0.15 No Hit TCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCG 6 0.15 No Hit GCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTG 6 0.15 No Hit TGGGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAAC 6 0.15 No Hit CTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAGCTGCAAC 6 0.15 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 6 0.15 No Hit ATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATA 6 0.15 No Hit CACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCG 6 0.15 No Hit TCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTT 6 0.15 No Hit TAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGA 6 0.15 No Hit GGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGG 6 0.15 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 5 0.125 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 5 0.125 No Hit GGCAGGGGTAGAAACTGCTGCGGTTAAGAAATTACAACCTTCCAAATAGG 5 0.125 No Hit GGGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGC 5 0.125 No Hit ACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 5 0.125 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 5 0.125 No Hit ATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCT 5 0.125 No Hit GTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCG 5 0.125 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 5 0.125 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 5 0.125 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 5 0.125 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 5 0.125 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 5 0.125 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 5 0.125 No Hit GAGCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTA 5 0.125 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 5 0.125 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 5 0.125 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 5 0.125 No Hit CCAGCCTTCTCCCCCAAAAGGGGATTTTAGTAAATAACCAAATATAACAC 5 0.125 No Hit AGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.05 0.0 0.0 0.0 0.0 86-87 0.05 0.0 0.0 0.0 0.0 88-89 0.05 0.0 0.0 0.0 0.0 90-91 0.05 0.0 0.0 0.0 0.0 92-93 0.05 0.0 0.0 0.0 0.0 94-95 0.05 0.0 0.0 0.0 0.0 96-97 0.05 0.0 0.0 0.0 0.0 98-99 0.05 0.0 0.0 0.0 0.0 100-101 0.075 0.0 0.0 0.0 0.0 102-103 0.1 0.0 0.0 0.0 0.0 104-105 0.1 0.0 0.0 0.0 0.0 106-107 0.1 0.0 0.0 0.0 0.0 108-109 0.1 0.0 0.0 0.0 0.0 110-111 0.1 0.0 0.0 0.0 0.0 112-113 0.1125 0.0 0.0 0.0 0.0 114-115 0.125 0.0 0.0 0.0 0.0 116-117 0.15 0.0 0.0 0.0 0.0 118-119 0.175 0.0 0.0 0.0 0.0 120-121 0.175 0.0 0.0 0.0 0.0 122-123 0.2 0.0 0.0 0.0 0.0 124-125 0.225 0.0 0.0 0.0 0.0 126-127 0.2375 0.0 0.0 0.0 0.0 128-129 0.30000000000000004 0.0 0.0 0.0 0.0 130-131 0.375 0.0 0.0 0.0 0.0 132-133 0.4875 0.0 0.0 0.0 0.0 134-135 0.5 0.0 0.0 0.0 0.0 136-137 0.525 0.0 0.0 0.0 0.0 138 0.55 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGTTCG 10 0.006973645 144.0 3 AACTTTA 10 0.006973645 144.0 4 GGTGGTT 10 0.006973645 144.0 1 GTGGTTC 10 0.006973645 144.0 2 TATAACA 20 3.687869E-4 108.0 5 CCCATAT 20 3.687869E-4 108.0 1 CCATATA 20 3.687869E-4 108.0 2 TCTTTTC 30 1.46121765E-5 96.0 5 GCTTTCT 30 1.46121765E-5 96.0 1 CTTTCTT 30 1.46121765E-5 96.0 2 ATAACAA 25 8.956223E-4 86.399994 6 CATATAA 25 8.956223E-4 86.399994 3 ATATAAC 25 8.956223E-4 86.399994 4 CTTTTCT 40 6.0911432E-5 72.0 6 TTTCTTT 40 6.0911432E-5 72.0 3 TTTCTTC 40 6.0911432E-5 72.0 8 TTTTCTT 40 6.0911432E-5 72.0 7 TTCTTCA 40 6.0911432E-5 72.0 9 TAACAAG 30 0.0018473949 72.0 7 ACAAGCT 30 0.0018473949 72.0 9 >>END_MODULE ERR11006583 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006583_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.7845 37.0 37.0 37.0 37.0 37.0 2 36.076 37.0 37.0 37.0 37.0 37.0 3 36.3915 37.0 37.0 37.0 37.0 37.0 4 36.25 37.0 37.0 37.0 37.0 37.0 5 36.371 37.0 37.0 37.0 37.0 37.0 6 36.2635 37.0 37.0 37.0 37.0 37.0 7 36.345 37.0 37.0 37.0 37.0 37.0 8 36.385 37.0 37.0 37.0 37.0 37.0 9 36.351 37.0 37.0 37.0 37.0 37.0 10-14 36.3011 37.0 37.0 37.0 37.0 37.0 15-19 36.2867 37.0 37.0 37.0 37.0 37.0 20-24 36.2392 37.0 37.0 37.0 37.0 37.0 25-29 36.2117 37.0 37.0 37.0 37.0 37.0 30-34 36.1437 37.0 37.0 37.0 37.0 37.0 35-39 36.1645 37.0 37.0 37.0 37.0 37.0 40-44 36.100199999999994 37.0 37.0 37.0 37.0 37.0 45-49 36.176 37.0 37.0 37.0 37.0 37.0 50-54 36.065 37.0 37.0 37.0 37.0 37.0 55-59 36.0374 37.0 37.0 37.0 37.0 37.0 60-64 35.940099999999994 37.0 37.0 37.0 37.0 37.0 65-69 35.9878 37.0 37.0 37.0 37.0 37.0 70-74 35.9461 37.0 37.0 37.0 37.0 37.0 75-79 35.88879999999999 37.0 37.0 37.0 37.0 37.0 80-84 35.8279 37.0 37.0 37.0 37.0 37.0 85-89 35.7074 37.0 37.0 37.0 37.0 37.0 90-94 35.7628 37.0 37.0 37.0 37.0 37.0 95-99 35.5939 37.0 37.0 37.0 37.0 37.0 100-104 35.610699999999994 37.0 37.0 37.0 37.0 37.0 105-109 35.4772 37.0 37.0 37.0 37.0 37.0 110-114 35.5002 37.0 37.0 37.0 37.0 37.0 115-119 35.3617 37.0 37.0 37.0 37.0 37.0 120-124 35.2057 37.0 37.0 37.0 32.2 37.0 125-129 35.3621 37.0 37.0 37.0 37.0 37.0 130-134 35.211999999999996 37.0 37.0 37.0 29.8 37.0 135-139 35.080200000000005 37.0 37.0 37.0 25.0 37.0 140-144 35.02570000000001 37.0 37.0 37.0 25.0 37.0 145-149 35.0774 37.0 37.0 37.0 27.4 37.0 150 34.826 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 0.0 16 0.0 17 0.0 18 0.0 19 2.0 20 1.0 21 1.0 22 2.0 23 6.0 24 5.0 25 10.0 26 15.0 27 12.0 28 26.0 29 31.0 30 34.0 31 60.0 32 86.0 33 118.0 34 208.0 35 524.0 36 2669.0 37 189.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.372210953346855 24.163286004056793 19.624746450304258 27.83975659229209 2 26.224999999999998 24.0 33.900000000000006 15.875 3 21.175 26.55 33.275 19.0 4 23.9 27.3 27.625 21.175 5 23.25 29.075 28.275 19.400000000000002 6 21.725 31.624999999999996 28.1 18.55 7 19.85 20.575 40.075 19.5 8 21.975 22.925 31.424999999999997 23.674999999999997 9 23.325000000000003 20.375 34.575 21.725 10-14 24.34 25.56 29.085 21.015 15-19 24.21 24.265 30.409999999999997 21.115000000000002 20-24 24.610000000000003 24.25 30.005 21.135 25-29 24.474999999999998 24.15 30.070000000000004 21.305 30-34 24.41 24.205 30.19 21.195 35-39 23.825 24.355 30.220000000000002 21.6 40-44 22.91 25.655 29.975 21.46 45-49 22.770000000000003 26.125 29.395 21.709999999999997 50-54 23.200000000000003 25.535000000000004 28.875 22.39 55-59 23.544999999999998 24.45 28.985 23.02 60-64 22.63 24.89 29.695 22.785 65-69 24.125 24.555 29.054999999999996 22.264999999999997 70-74 23.849999999999998 24.55 30.03 21.57 75-79 24.91 24.275 30.044999999999998 20.77 80-84 24.884999999999998 25.055 28.585 21.475 85-89 24.69 24.495 28.54 22.275 90-94 23.615 24.775 28.98 22.63 95-99 24.05 23.585 29.720000000000002 22.645 100-104 24.295 23.075000000000003 30.56 22.07 105-109 25.474999999999998 22.665 30.09 21.77 110-114 23.665 23.705000000000002 30.34 22.29 115-119 24.29 25.105 28.615000000000002 21.990000000000002 120-124 24.355 24.855 28.634999999999998 22.155 125-129 22.755 25.169999999999998 29.42 22.655 130-134 22.61 25.495 29.025000000000002 22.869999999999997 135-139 23.755000000000003 24.995 29.86 21.39 140-144 23.565 25.135 29.725 21.575 145-149 22.97 24.25 30.814999999999998 21.965 150 24.3 23.7 28.625 23.375 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.5 21 2.0 22 1.5 23 1.5 24 3.5 25 9.5 26 10.5 27 11.0 28 10.5 29 18.0 30 28.5 31 26.0 32 24.0 33 27.5 34 45.0 35 75.5 36 104.0 37 181.5 38 242.5 39 234.5 40 239.5 41 247.5 42 235.5 43 228.0 44 247.0 45 230.5 46 189.0 47 142.5 48 104.0 49 88.0 50 73.5 51 59.5 52 45.0 53 38.5 54 41.0 55 39.5 56 31.0 57 32.0 58 29.0 59 29.5 60 39.5 61 38.0 62 34.5 63 47.0 64 61.0 65 59.5 66 43.0 67 30.0 68 28.0 69 28.0 70 26.5 71 19.0 72 18.0 73 23.5 74 16.0 75 10.5 76 11.5 77 11.0 78 7.0 79 2.0 80 2.5 81 4.0 82 2.0 83 1.5 84 2.0 85 2.0 86 1.5 87 0.5 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.4000000000000001 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.25 #Duplication Level Percentage of deduplicated Percentage of total 1 79.73977695167285 53.625 2 11.933085501858736 16.05 3 3.2713754646840147 6.6000000000000005 4 1.8959107806691449 5.1 5 0.7434944237918215 2.5 6 0.5947955390334573 2.4 7 0.5947955390334573 2.8000000000000003 8 0.18587360594795538 1.0 9 0.26022304832713755 1.575 >10 0.7434944237918215 6.9 >50 0.03717472118959108 1.4500000000000002 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 58 1.4500000000000002 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 28 0.7000000000000001 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 25 0.625 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 17 0.42500000000000004 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 14 0.35000000000000003 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 14 0.35000000000000003 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 14 0.35000000000000003 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 14 0.35000000000000003 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 13 0.325 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 13 0.325 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 12 0.3 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 12 0.3 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 12 0.3 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 12 0.3 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 12 0.3 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 12 0.3 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 11 0.27499999999999997 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 11 0.27499999999999997 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 10 0.25 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 10 0.25 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 10 0.25 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 9 0.22499999999999998 No Hit AGCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTG 9 0.22499999999999998 No Hit TATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACC 9 0.22499999999999998 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 9 0.22499999999999998 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 9 0.22499999999999998 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 9 0.22499999999999998 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 9 0.22499999999999998 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 8 0.2 No Hit AACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGG 8 0.2 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 8 0.2 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 8 0.2 No Hit GAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTA 8 0.2 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 7 0.17500000000000002 No Hit CCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGG 7 0.17500000000000002 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 7 0.17500000000000002 No Hit AATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTAT 7 0.17500000000000002 No Hit TCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGA 7 0.17500000000000002 No Hit ATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGT 7 0.17500000000000002 No Hit AATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGC 7 0.17500000000000002 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 7 0.17500000000000002 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 7 0.17500000000000002 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 7 0.17500000000000002 No Hit CTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTTATGA 7 0.17500000000000002 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 7 0.17500000000000002 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 7 0.17500000000000002 No Hit CTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTAC 7 0.17500000000000002 No Hit TCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTG 7 0.17500000000000002 No Hit AGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGC 7 0.17500000000000002 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 6 0.15 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 6 0.15 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 6 0.15 No Hit CTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACC 6 0.15 No Hit ATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATAT 6 0.15 No Hit GTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCAT 6 0.15 No Hit CTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTA 6 0.15 No Hit AGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCT 6 0.15 No Hit TGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATC 6 0.15 No Hit ATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAG 6 0.15 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 6 0.15 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 6 0.15 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 6 0.15 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 6 0.15 No Hit CTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTC 6 0.15 No Hit CTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTC 6 0.15 No Hit GGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTT 5 0.125 No Hit TTCACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCT 5 0.125 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 5 0.125 No Hit ATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCT 5 0.125 No Hit CAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGT 5 0.125 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 5 0.125 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 5 0.125 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 5 0.125 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 5 0.125 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 5 0.125 No Hit CGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTT 5 0.125 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 5 0.125 No Hit GCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTAT 5 0.125 No Hit AATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGT 5 0.125 No Hit GTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACT 5 0.125 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 5 0.125 No Hit CTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGAT 5 0.125 No Hit GGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACA 5 0.125 No Hit ATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTG 5 0.125 No Hit GTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.05 0.0 0.0 0.0 0.0 86-87 0.05 0.0 0.0 0.0 0.0 88-89 0.05 0.0 0.0 0.0 0.0 90-91 0.05 0.0 0.0 0.0 0.0 92-93 0.05 0.0 0.0 0.0 0.0 94-95 0.05 0.0 0.0 0.0 0.0 96-97 0.05 0.0 0.0 0.0 0.0 98-99 0.05 0.0 0.0 0.0 0.0 100-101 0.075 0.0 0.0 0.0 0.0 102-103 0.1 0.0 0.0 0.0 0.0 104-105 0.1 0.0 0.0 0.0 0.0 106-107 0.1 0.0 0.0 0.0 0.0 108-109 0.1 0.0 0.0 0.0 0.0 110-111 0.1 0.0 0.0 0.0 0.0 112-113 0.1125 0.0 0.0 0.0 0.0 114-115 0.125 0.0 0.0 0.0 0.0 116-117 0.1375 0.0 0.0 0.0 0.0 118-119 0.15 0.0 0.0 0.0 0.0 120-121 0.15 0.0 0.0 0.0 0.0 122-123 0.175 0.0 0.0 0.0 0.0 124-125 0.2 0.0 0.0 0.0 0.0 126-127 0.21250000000000002 0.0 0.0 0.0 0.0 128-129 0.275 0.0 0.0 0.0 0.0 130-131 0.35 0.0 0.0 0.0 0.0 132-133 0.4625 0.0 0.0 0.0 0.0 134-135 0.475 0.0 0.0 0.0 0.0 136-137 0.5 0.0 0.0 0.0 0.0 138 0.525 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTATTT 15 1.1150019E-4 145.81013 1 TATTTCT 20 3.689142E-4 107.99063 3 TTCTAGT 25 8.959314E-4 86.392494 6 ATTTCTA 25 8.959314E-4 86.392494 4 TTTCTAG 30 0.0018480307 71.99375 5 TTATTTC 30 0.0018480307 71.99375 2 TAGTTAA 35 0.0034056995 61.708927 9 TCTAGTT 40 0.0057794205 53.995316 7 CTAGTTA 40 0.0057794205 53.995316 8 TAATATG 30 0.0015039494 23.997917 6 CATGACT 35 0.0036832115 20.569643 60-64 ATGACTG 35 0.0036832115 20.569643 60-64 AATTTGA 35 0.0036832115 20.569643 35-39 TTTTAGA 35 0.0036832115 20.569643 70-74 CTTGGGA 35 0.0036832115 20.569643 20-24 AACCAAG 35 0.0036832115 20.569643 45-49 CTTACCA 35 0.0036832115 20.569643 55-59 AGAGAGA 35 0.0036832115 20.569643 75-79 GCTTGGG 35 0.0036832115 20.569643 20-24 GAGAGAC 35 0.0036832115 20.569643 75-79 >>END_MODULE Read 2540773 spots for ERR11006583.sra Written 2540773 spots for ERR11006583.sra Read 2540773 spots for ERR11006583.sra Written 2540773 spots for ERR11006583.sra Read 2540773 spots for ERR11006583.sra Written 2540773 spots for ERR11006583.sra Read 2540773 spots for ERR11006583.sra Written 2540773 spots for ERR11006583.sra Read 2540773 spots for ERR11006583.sra Written 2540773 spots for ERR11006583.sra Read 2540773 spots for ERR11006583.sra Written 2540773 spots for ERR11006583.sra Read 2540773 spots for ERR11006583.sra Written 2540773 spots for ERR11006583.sra Read 2540773 spots for ERR11006583.sra Written 2540773 spots for ERR11006583.sra Read 2540773 spots for ERR11006583.sra Written 2540773 spots for ERR11006583.sra Read 2540773 spots for ERR11006583.sra Written 2540773 spots for ERR11006583.sra Read 2540773 spots for ERR11006583.sra Written 2540773 spots for ERR11006583.sra Read 2540773 spots for ERR11006583.sra Written 2540773 spots for ERR11006583.sra Read 2540773 spots for ERR11006583.sra Written 2540773 spots for ERR11006583.sra Read 2540773 spots for ERR11006583.sra Written 2540773 spots for ERR11006583.sra Read 2540773 spots for ERR11006583.sra Written 2540773 spots for ERR11006583.sra Read 2540773 spots for ERR11006583.sra Written 2540773 spots for ERR11006583.sra Read 2540773 spots for ERR11006583.sra Written 2540773 spots for ERR11006583.sra Read 2540773 spots for ERR11006583.sra Written 2540773 spots for ERR11006583.sra Read 2540784 spots for ERR11006583.sra Written 2540784 spots for ERR11006583.sra Read 2540773 spots for ERR11006583.sra Written 2540773 spots for ERR11006583.sra SRR ids: ['ERR11006583.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_wagnui8o ERR11006583.sra spots: 50815471 blocks: [[1, 2540773], [2540774, 5081546], [5081547, 7622319], [7622320, 10163092], [10163093, 12703865], [12703866, 15244638], [15244639, 17785411], [17785412, 20326184], [20326185, 22866957], [22866958, 25407730], [25407731, 27948503], [27948504, 30489276], [30489277, 33030049], [33030050, 35570822], [35570823, 38111595], [38111596, 40652368], [40652369, 43193141], [43193142, 45733914], [45733915, 48274687], [48274688, 50815471]] ERR11006583 file size 18671094 ERR11006583 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006583 ERR11006583_1.fastq ERR11006583_2.fastq Input file: ERR11006583_1.fastq Paired file: ERR11006583_2.fastq trimmed: ERR11006583-trimmed-pair1.fastq, ERR11006583-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 18:18:48 2024 >> started Fri Dec 6 18:19:53 2024 >> done (65.077s) 50815471 read pairs processed; of these: 101 ( 0.00%) short read pairs filtered out after trimming by size control 600 ( 0.00%) empty read pairs filtered out after trimming by size control 50814770 (100.00%) read pairs available; of these: 498554 ( 0.98%) trimmed read pairs available after processing 50316216 (99.02%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 1 0.00% 20 12 0.00% 21 5 0.00% 22 17 0.00% 23 10 0.00% 24 3 0.00% 25 61 0.00% 26 13 0.00% 27 12 0.00% 28 11 0.00% 29 21 0.00% 30 11 0.00% 31 36 0.00% 32 13 0.00% 33 14 0.00% 34 15 0.00% 35 14 0.00% 36 14 0.00% 37 20 0.00% 38 19 0.00% 39 17 0.00% 40 26 0.00% 41 22 0.00% 42 20 0.00% 43 21 0.00% 44 20 0.00% 45 35 0.00% 46 24 0.00% 47 37 0.00% 48 32 0.00% 49 44 0.00% 50 37 0.00% 51 46 0.00% 52 59 0.00% 53 64 0.00% 54 54 0.00% 55 84 0.00% 56 78 0.00% 57 81 0.00% 58 72 0.00% 59 90 0.00% 60 99 0.00% 61 106 0.00% 62 130 0.00% 63 149 0.00% 64 171 0.00% 65 153 0.00% 66 187 0.00% 67 211 0.00% 68 212 0.00% 69 235 0.00% 70 238 0.00% 71 261 0.00% 72 286 0.00% 73 315 0.00% 74 360 0.00% 75 403 0.00% 76 405 0.00% 77 436 0.00% 78 444 0.00% 79 508 0.00% 80 511 0.00% 81 581 0.00% 82 643 0.00% 83 711 0.00% 84 772 0.00% 85 911 0.00% 86 932 0.00% 87 960 0.00% 88 1072 0.00% 89 1115 0.00% 90 1224 0.00% 91 1208 0.00% 92 1329 0.00% 93 1375 0.00% 94 1555 0.00% 95 1702 0.00% 96 1741 0.00% 97 1849 0.00% 98 2119 0.00% 99 2054 0.00% 100 2208 0.00% 101 2357 0.00% 102 2475 0.00% 103 2689 0.01% 104 2774 0.01% 105 3046 0.01% 106 3225 0.01% 107 3298 0.01% 108 3440 0.01% 109 3660 0.01% 110 4004 0.01% 111 3998 0.01% 112 4553 0.01% 113 4496 0.01% 114 4641 0.01% 115 4978 0.01% 116 5515 0.01% 117 5707 0.01% 118 5684 0.01% 119 6149 0.01% 120 6821 0.01% 121 7101 0.01% 122 7687 0.02% 123 7665 0.02% 124 8069 0.02% 125 9143 0.02% 126 8442 0.02% 127 8133 0.02% 128 9222 0.02% 129 9959 0.02% 130 10656 0.02% 131 11046 0.02% 132 11502 0.02% 133 11166 0.02% 134 11684 0.02% 135 11649 0.02% 136 12419 0.02% 137 12941 0.03% 138 13595 0.03% 139 14289 0.03% 140 14409 0.03% 141 15645 0.03% 142 16270 0.03% 143 17154 0.03% 144 17067 0.03% 145 18943 0.04% 146 22290 0.04% 147 20457 0.04% 148 21165 0.04% 149 22106 0.04% 150 50316216 99.02% 50814770 reads passed initial QC criterion=sequence-density sequence-density=0.24 sequence-density-rank=1 fanout-score=0.00 fanout-score-rank=29 prefix-density=0.00 prefix-fanout=1.0 sequence=TGCGGGAACTTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=27 fanout-score=66.80 fanout-score-rank=1 prefix-density=0.19 prefix-fanout=4.9 sequence=ATAAAAAAAAGGGGGGGTAAGGACCCGCTAAGCTCCTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGATGAACCCAATCCAGAATATGAACCATAAAAGAAAACACCTACTAAACCAATCACAAGAATACCAGTTACCGTACCTATCAGCCAAAGAGGAATTCTTCCAGTAGTATCGGCCATTTCCCCTACTTTCCTCCACATTTTATCAAGTGGTCATGCTAGAGACAAAAACAGTCATGGATAGTTATGTTATAAGGATGGTATCCTTCCAAATGGGATAAGAGAGTTCTTACTACTCTCTTCTTTTCTCTCAATTAAAGAAGTAATTGGAAAACAAAACAGCAAGTACAAAAATGAGTAATAAACCCCAGTATAGACTGGTACGATTCAATTCAACATTTTGTTCATTCGGGTTTGATTGTGTCATAGTTCTATAGTTGGAATTTAGTTTATCGTTGGATGAACTGCATTGCTGATATTGATCCCAAGAAAAAAA criterion=sequence-density sequence-density=0.20 sequence-density-rank=1 fanout-score=13.22 fanout-score-rank=12 prefix-density=2.69 prefix-fanout=1.0 sequence=TTGCGTAGTGGATCTGCTGGGGCCTATGCGAAAGCTGGGCCTCACGAATTCTATAGTGGCAGGCACCGCGTTAGGCTGGCTTC criterion=fanout-score sequence-density=0.07 sequence-density-rank=21 fanout-score=169.38 fanout-score-rank=1 prefix-density=10.68 prefix-fanout=1.0 sequence=AGCACTGAAAAACGTCTTTACAT ERR11006583 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 18:22:44 Started mapping on | Dec 06 18:22:44 Finished on | Dec 06 18:28:35 Mapping speed, Million of reads per hour | 521.18 Number of input reads | 50814770 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 39953124 Uniquely mapped reads % | 78.63% Average mapped length | 298.44 Number of splices: Total | 13838912 Number of splices: Annotated (sjdb) | 12940686 Number of splices: GT/AG | 13534682 Number of splices: GC/AG | 168862 Number of splices: AT/AC | 16768 Number of splices: Non-canonical | 118600 Mismatch rate per base, % | 0.30% Deletion rate per base | 0.01% Deletion average length | 1.73 Insertion rate per base | 0.01% Insertion average length | 2.17 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 9280636 % of reads mapped to multiple loci | 18.26% Number of reads mapped to too many loci | 11399 % of reads mapped to too many loci | 0.02% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.70% % of reads unmapped: other | 0.38% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1581010 1581010 1581010 N_multimapping 9280636 9280636 9280636 N_noFeature 10633688 37173476 12596515 N_ambiguous 1294339 37428 475347 UnstrandedReadsAssigned:28025097 PositiveStrandReadsAssigned:2742220 NegativeStrandReadsAssigned:26881262 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006583 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006583-trimmed-pair1.fastq ERR11006583-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 50,814,770 reads, 29,125,163 reads pseudoaligned [quant] estimated average fragment length: 273.053 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,129 rounds 52973 ERR11006583.ke.tsv 35125 ERR11006583.se.tsv 88098 total ==> ERR11006583.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 664.296 0 0 PNS24247 1044 771.947 25.2656 1.13284 PNS24249 1928 1655.95 129.673 2.71038 PNS24246 1044 771.947 25.2656 1.13284 PNS24248 1044 771.947 25.2656 1.13284 PNS24244 1471 1198.95 46.5297 1.34325 PNS24243 293 53.7045 3 1.93346 KQK14069 1603 1330.95 1379.94 35.886 KQK14071 474 204.637 22.7738 3.85191 ==> ERR11006583.se.tsv <== BRADI_1g14170v3 1560 BRADI_1g53295v3 152 BRADI_1g59795v3 198 BRADI_1g07683v3 0 BRADI_1g00485v3 4 BRADI_1g20270v3 487 BRADI_1g74790v3 344 BRADI_1g09890v3 5 BRADI_1g77505v3 254 BRADI_1g48960v3 6 ERR11006583 completed mapping pipeline successfully