Starting /dee2/code/volunteer_pipeline.sh ERR11006584 current disk space = 1550659960832 free memory = 1598832588 ERR11006584 SRAfilesize 00321773bfdae15631fb44644f2c60df ERR11006584.sra ERR11006584.sra file validated ERR11006584 is paired end ERR11006584 is conventional basespace ERR11006584 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006584_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2755 37.0 37.0 37.0 37.0 37.0 2 36.198 37.0 37.0 37.0 37.0 37.0 3 36.352 37.0 37.0 37.0 37.0 37.0 4 36.448 37.0 37.0 37.0 37.0 37.0 5 36.3715 37.0 37.0 37.0 37.0 37.0 6 36.48 37.0 37.0 37.0 37.0 37.0 7 36.377 37.0 37.0 37.0 37.0 37.0 8 36.395 37.0 37.0 37.0 37.0 37.0 9 36.347 37.0 37.0 37.0 37.0 37.0 10-14 36.4113 37.0 37.0 37.0 37.0 37.0 15-19 36.4454 37.0 37.0 37.0 37.0 37.0 20-24 36.3863 37.0 37.0 37.0 37.0 37.0 25-29 36.35549999999999 37.0 37.0 37.0 37.0 37.0 30-34 36.3112 37.0 37.0 37.0 37.0 37.0 35-39 36.3216 37.0 37.0 37.0 37.0 37.0 40-44 36.3134 37.0 37.0 37.0 37.0 37.0 45-49 36.239399999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.2778 37.0 37.0 37.0 37.0 37.0 55-59 36.209900000000005 37.0 37.0 37.0 37.0 37.0 60-64 36.178 37.0 37.0 37.0 37.0 37.0 65-69 36.1814 37.0 37.0 37.0 37.0 37.0 70-74 36.149100000000004 37.0 37.0 37.0 37.0 37.0 75-79 36.0378 37.0 37.0 37.0 37.0 37.0 80-84 36.056200000000004 37.0 37.0 37.0 37.0 37.0 85-89 36.016 37.0 37.0 37.0 37.0 37.0 90-94 35.943599999999996 37.0 37.0 37.0 37.0 37.0 95-99 36.0304 37.0 37.0 37.0 37.0 37.0 100-104 36.005199999999995 37.0 37.0 37.0 37.0 37.0 105-109 35.877700000000004 37.0 37.0 37.0 37.0 37.0 110-114 35.8273 37.0 37.0 37.0 37.0 37.0 115-119 35.7958 37.0 37.0 37.0 37.0 37.0 120-124 35.856 37.0 37.0 37.0 37.0 37.0 125-129 35.6524 37.0 37.0 37.0 37.0 37.0 130-134 35.653600000000004 37.0 37.0 37.0 37.0 37.0 135-139 35.548500000000004 37.0 37.0 37.0 37.0 37.0 140-144 35.3223 37.0 37.0 37.0 32.2 37.0 145-149 35.4367 37.0 37.0 37.0 37.0 37.0 150 35.399 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 0.0 23 1.0 24 2.0 25 4.0 26 8.0 27 21.0 28 14.0 29 30.0 30 59.0 31 59.0 32 75.0 33 90.0 34 139.0 35 319.0 36 2855.0 37 323.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.625 13.025 15.2 34.150000000000006 2 30.2 10.424999999999999 27.375 32.0 3 24.025 13.075000000000001 27.825 35.075 4 27.525 16.375 25.2 30.9 5 29.95 19.25 24.05 26.75 6 26.200000000000003 28.499999999999996 19.375 25.924999999999997 7 18.7 29.5 33.650000000000006 18.15 8 19.025 28.075 33.15 19.75 9 18.275 27.400000000000002 34.375 19.950000000000003 10-14 20.82 31.05 26.685 21.445 15-19 21.665 32.684999999999995 23.830000000000002 21.82 20-24 19.75 28.335 27.544999999999998 24.37 25-29 22.509999999999998 30.97 24.535 21.985 30-34 23.54 30.705 23.615 22.14 35-39 24.265 30.535 23.61 21.59 40-44 21.310000000000002 30.175 24.625 23.89 45-49 21.65 28.955 25.995 23.400000000000002 50-54 20.93 31.715 25.674999999999997 21.68 55-59 21.91 29.195 23.474999999999998 25.419999999999998 60-64 20.79 30.845 24.34 24.025 65-69 21.915000000000003 29.439999999999998 24.62 24.025 70-74 24.695 29.080000000000002 21.735 24.490000000000002 75-79 22.91 29.854999999999997 24.33 22.905 80-84 23.215 28.425 24.55 23.810000000000002 85-89 23.505000000000003 28.610000000000003 25.145 22.74 90-94 22.75 29.335 25.11 22.805 95-99 24.54 28.470000000000002 23.005 23.985 100-104 21.73 30.354999999999997 23.849999999999998 24.065 105-109 22.615 27.860000000000003 25.674999999999997 23.849999999999998 110-114 23.735 27.48 24.59 24.195 115-119 21.44 30.225 24.88 23.455000000000002 120-124 20.31 29.970000000000002 24.685000000000002 25.035 125-129 21.34 31.055 22.25 25.355 130-134 23.59 29.915000000000003 24.05 22.445 135-139 24.11 28.810000000000002 22.470000000000002 24.610000000000003 140-144 24.54 28.799999999999997 24.875 21.785 145-149 23.69 29.255 24.39 22.665 150 23.575 27.224999999999998 27.650000000000002 21.55 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 0.0 22 2.0 23 2.5 24 1.0 25 2.0 26 2.5 27 3.5 28 6.5 29 10.5 30 16.0 31 19.0 32 21.0 33 32.0 34 36.5 35 41.5 36 65.5 37 155.5 38 282.0 39 261.0 40 215.0 41 258.0 42 262.5 43 240.0 44 221.0 45 231.5 46 218.5 47 154.5 48 105.5 49 89.0 50 77.0 51 55.0 52 49.5 53 51.5 54 41.0 55 33.0 56 32.5 57 30.0 58 30.5 59 33.5 60 37.0 61 28.0 62 21.0 63 30.5 64 71.0 65 113.0 66 76.5 67 28.0 68 26.0 69 26.5 70 22.5 71 21.0 72 19.5 73 15.5 74 12.5 75 10.5 76 10.5 77 11.0 78 8.0 79 5.0 80 4.5 81 3.0 82 2.5 83 2.0 84 0.0 85 0.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.5 #Duplication Level Percentage of deduplicated Percentage of total 1 80.15503875968992 51.7 2 12.635658914728682 16.3 3 3.0232558139534884 5.8500000000000005 4 1.0465116279069768 2.7 5 0.7364341085271318 2.375 6 0.46511627906976744 1.7999999999999998 7 0.5038759689922481 2.275 8 0.23255813953488372 1.2 9 0.23255813953488372 1.35 >10 0.9302325581395349 11.55 >50 0.0 0.0 >100 0.03875968992248062 2.9000000000000004 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 116 2.9000000000000004 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 42 1.05 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 31 0.775 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 30 0.75 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 30 0.75 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 29 0.7250000000000001 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 27 0.675 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 23 0.575 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 22 0.5499999999999999 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 21 0.525 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 20 0.5 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 18 0.44999999999999996 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 18 0.44999999999999996 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 16 0.4 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 15 0.375 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 15 0.375 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 13 0.325 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 13 0.325 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 13 0.325 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 13 0.325 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 11 0.27499999999999997 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 11 0.27499999999999997 No Hit CGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAAC 11 0.27499999999999997 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 10 0.25 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 10 0.25 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 9 0.22499999999999998 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 9 0.22499999999999998 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 9 0.22499999999999998 No Hit TGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAG 9 0.22499999999999998 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 9 0.22499999999999998 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 9 0.22499999999999998 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 8 0.2 No Hit GTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGC 8 0.2 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 8 0.2 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 8 0.2 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 8 0.2 No Hit CAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTG 8 0.2 No Hit GGGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACA 7 0.17500000000000002 No Hit TGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATA 7 0.17500000000000002 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 7 0.17500000000000002 No Hit TCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGT 7 0.17500000000000002 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 7 0.17500000000000002 No Hit GGCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGAT 7 0.17500000000000002 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 7 0.17500000000000002 No Hit GCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAGCTGCAA 7 0.17500000000000002 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 7 0.17500000000000002 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 7 0.17500000000000002 No Hit AGGCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGA 7 0.17500000000000002 No Hit GGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGG 7 0.17500000000000002 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 7 0.17500000000000002 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 6 0.15 No Hit CTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAAT 6 0.15 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 6 0.15 No Hit TGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGG 6 0.15 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 6 0.15 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 6 0.15 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 6 0.15 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 6 0.15 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 6 0.15 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 6 0.15 No Hit CACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAAT 6 0.15 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 6 0.15 No Hit TCTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTA 5 0.125 No Hit ACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTG 5 0.125 No Hit GCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCC 5 0.125 No Hit GCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCA 5 0.125 No Hit TCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGT 5 0.125 No Hit CGCTGCTCTGTGAAGCCAGCCTCACGCTGTGCCTGCCAACATTATGGGCC 5 0.125 No Hit AGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 5 0.125 No Hit ATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATA 5 0.125 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 5 0.125 No Hit CCACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAA 5 0.125 No Hit CAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGA 5 0.125 No Hit TCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATC 5 0.125 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 5 0.125 No Hit AGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTAT 5 0.125 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 5 0.125 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 5 0.125 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 5 0.125 No Hit CTTCCATACCAAGATTAGCACGGTTGATGATATCAGCCCAAGTATTAATA 5 0.125 No Hit GCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.05 0.0 0.0 0.0 0.0 84-85 0.05 0.0 0.0 0.0 0.0 86-87 0.05 0.0 0.0 0.0 0.0 88-89 0.05 0.0 0.0 0.0 0.0 90-91 0.075 0.0 0.0 0.0 0.0 92-93 0.075 0.0 0.0 0.0 0.0 94-95 0.075 0.0 0.0 0.0 0.0 96-97 0.075 0.0 0.0 0.0 0.0 98-99 0.075 0.0 0.0 0.0 0.0 100-101 0.1 0.0 0.0 0.0 0.0 102-103 0.1 0.0 0.0 0.0 0.0 104-105 0.1 0.0 0.0 0.0 0.0 106-107 0.125 0.0 0.0 0.0 0.0 108-109 0.125 0.0 0.0 0.0 0.0 110-111 0.125 0.0 0.0 0.0 0.0 112-113 0.125 0.0 0.0 0.0 0.0 114-115 0.125 0.0 0.0 0.0 0.0 116-117 0.15 0.0 0.0 0.0 0.0 118-119 0.15 0.0 0.0 0.0 0.0 120-121 0.15 0.0 0.0 0.0 0.0 122-123 0.175 0.0 0.0 0.0 0.0 124-125 0.2125 0.0 0.0 0.0 0.0 126-127 0.275 0.0 0.0 0.0 0.0 128-129 0.2875 0.0 0.0 0.0 0.0 130-131 0.3 0.0 0.0 0.0 0.0 132-133 0.3375 0.0 0.0 0.0 0.0 134-135 0.375 0.0 0.0 0.0 0.0 136-137 0.525 0.0 0.0 0.0 0.0 138 0.55 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTTCT 25 8.956223E-4 86.399994 6 TCTTTTC 25 8.956223E-4 86.399994 5 GCTTTCT 25 8.956223E-4 86.399994 1 TTTCTTT 30 0.0018473949 72.0 3 TTTCTTC 30 0.0018473949 72.0 8 TTTTCTT 30 0.0018473949 72.0 7 TTCTTTT 30 0.0018473949 72.0 4 TTCTTCA 35 0.0034045284 61.714283 9 CTTTCTT 40 0.005777437 54.0 2 AAAATTC 30 0.0015031899 23.999998 15-19 AAAAATT 30 0.0015031899 23.999998 15-19 >>END_MODULE ERR11006584 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006584_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.5435 37.0 37.0 37.0 37.0 37.0 2 36.106 37.0 37.0 37.0 37.0 37.0 3 36.1215 37.0 37.0 37.0 37.0 37.0 4 36.2845 37.0 37.0 37.0 37.0 37.0 5 36.157 37.0 37.0 37.0 37.0 37.0 6 36.217 37.0 37.0 37.0 37.0 37.0 7 36.1905 37.0 37.0 37.0 37.0 37.0 8 36.3585 37.0 37.0 37.0 37.0 37.0 9 36.3295 37.0 37.0 37.0 37.0 37.0 10-14 36.3155 37.0 37.0 37.0 37.0 37.0 15-19 36.29639999999999 37.0 37.0 37.0 37.0 37.0 20-24 36.258500000000005 37.0 37.0 37.0 37.0 37.0 25-29 36.217200000000005 37.0 37.0 37.0 37.0 37.0 30-34 36.1352 37.0 37.0 37.0 37.0 37.0 35-39 36.1466 37.0 37.0 37.0 37.0 37.0 40-44 36.142199999999995 37.0 37.0 37.0 37.0 37.0 45-49 36.131 37.0 37.0 37.0 37.0 37.0 50-54 36.0713 37.0 37.0 37.0 37.0 37.0 55-59 36.053700000000006 37.0 37.0 37.0 37.0 37.0 60-64 35.9408 37.0 37.0 37.0 37.0 37.0 65-69 35.899699999999996 37.0 37.0 37.0 37.0 37.0 70-74 35.8662 37.0 37.0 37.0 37.0 37.0 75-79 35.8194 37.0 37.0 37.0 37.0 37.0 80-84 35.784299999999995 37.0 37.0 37.0 37.0 37.0 85-89 35.7265 37.0 37.0 37.0 37.0 37.0 90-94 35.7464 37.0 37.0 37.0 37.0 37.0 95-99 35.60730000000001 37.0 37.0 37.0 37.0 37.0 100-104 35.5878 37.0 37.0 37.0 37.0 37.0 105-109 35.399699999999996 37.0 37.0 37.0 37.0 37.0 110-114 35.4119 37.0 37.0 37.0 37.0 37.0 115-119 35.3595 37.0 37.0 37.0 37.0 37.0 120-124 35.255399999999995 37.0 37.0 37.0 29.8 37.0 125-129 35.2675 37.0 37.0 37.0 29.8 37.0 130-134 35.1717 37.0 37.0 37.0 27.4 37.0 135-139 35.035700000000006 37.0 37.0 37.0 25.0 37.0 140-144 34.838499999999996 37.0 37.0 37.0 25.0 37.0 145-149 35.025800000000004 37.0 37.0 37.0 25.0 37.0 150 34.732 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 0.0 17 1.0 18 1.0 19 1.0 20 1.0 21 3.0 22 3.0 23 3.0 24 9.0 25 9.0 26 10.0 27 20.0 28 31.0 29 24.0 30 43.0 31 52.0 32 84.0 33 103.0 34 202.0 35 593.0 36 2632.0 37 174.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.93370729219786 24.400815910249875 16.598674145843958 29.06680265170831 2 26.55 24.55 32.925 15.975 3 20.45 26.625 32.85 20.075000000000003 4 23.95 27.625 26.400000000000002 22.025 5 24.55 29.4 27.700000000000003 18.35 6 21.25 31.65 26.6 20.5 7 20.225 21.099999999999998 39.800000000000004 18.875 8 22.900000000000002 22.575 31.4 23.125 9 23.625 20.275000000000002 34.925 21.175 10-14 24.46 25.835 28.194999999999997 21.51 15-19 24.834999999999997 24.635 29.360000000000003 21.17 20-24 26.279999999999998 23.49 29.165000000000003 21.065 25-29 24.86 23.835 29.794999999999998 21.51 30-34 24.87 23.51 29.705 21.915000000000003 35-39 25.395 23.815 28.825 21.965 40-44 24.08 24.565 29.395 21.959999999999997 45-49 24.485 25.095 28.83 21.59 50-54 24.395 24.725 27.439999999999998 23.44 55-59 23.94 24.415 28.749999999999996 22.895 60-64 23.995 24.205 29.09 22.71 65-69 25.09 23.91 28.255000000000003 22.745 70-74 24.38 24.975 29.235 21.41 75-79 24.695 24.01 29.26 22.035 80-84 25.124999999999996 23.96 29.42 21.495 85-89 25.259999999999998 24.515 26.939999999999998 23.285 90-94 24.505 24.12 28.415000000000003 22.96 95-99 23.615 24.415 28.939999999999998 23.03 100-104 24.4 23.544999999999998 30.085 21.97 105-109 26.325 23.01 28.63 22.035 110-114 24.805 23.630000000000003 29.020000000000003 22.545 115-119 24.3 24.62 28.875 22.205 120-124 24.645 24.740000000000002 27.575 23.04 125-129 23.815 25.3 28.76 22.125 130-134 22.74 25.919999999999998 28.799999999999997 22.54 135-139 23.89 24.58 29.89 21.64 140-144 23.995 24.66 29.025000000000002 22.32 145-149 22.8 23.855 30.225 23.119999999999997 150 23.0 23.05 29.5 24.45 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 1.0 19 1.5 20 0.5 21 0.0 22 0.0 23 2.0 24 3.5 25 7.5 26 10.5 27 10.5 28 14.0 29 17.5 30 19.5 31 22.5 32 26.0 33 31.5 34 51.5 35 79.0 36 107.0 37 178.0 38 217.5 39 186.5 40 211.5 41 241.5 42 209.0 43 198.5 44 222.5 45 222.0 46 196.5 47 155.0 48 112.5 49 77.5 50 67.5 51 65.0 52 49.5 53 44.5 54 37.0 55 36.5 56 41.0 57 36.0 58 30.0 59 36.5 60 40.5 61 34.0 62 35.5 63 76.0 64 106.0 65 95.5 66 64.5 67 33.5 68 30.5 69 27.5 70 20.0 71 22.5 72 28.0 73 22.0 74 15.0 75 15.5 76 11.0 77 8.0 78 7.5 79 5.0 80 5.0 81 3.0 82 3.0 83 4.5 84 2.0 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.95 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.025 #Duplication Level Percentage of deduplicated Percentage of total 1 79.14957105557627 53.05 2 12.607236105930623 16.900000000000002 3 3.282357329354718 6.6000000000000005 4 1.6038791495710556 4.3 5 0.969787392763894 3.25 6 0.5221932114882507 2.1 7 0.5967922417008579 2.8000000000000003 8 0.37299515106303616 2.0 9 0.26109660574412535 1.575 >10 0.5967922417008579 6.125 >50 0.03729951510630362 1.3 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 52 1.3 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 31 0.775 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 21 0.525 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 21 0.525 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 20 0.5 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 18 0.44999999999999996 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 15 0.375 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 15 0.375 No Hit GCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGC 14 0.35000000000000003 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 12 0.3 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 12 0.3 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 12 0.3 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 12 0.3 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 11 0.27499999999999997 No Hit GAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCG 11 0.27499999999999997 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 10 0.25 No Hit AGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGC 10 0.25 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 9 0.22499999999999998 No Hit GATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTC 9 0.22499999999999998 No Hit CTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAG 9 0.22499999999999998 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 9 0.22499999999999998 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 9 0.22499999999999998 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 9 0.22499999999999998 No Hit GTGGACGTTGCCGTAGCGCTGCGGGCCTGGTCTGGGTGTGCTACTGATGG 9 0.22499999999999998 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 8 0.2 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 8 0.2 No Hit GTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTG 8 0.2 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 8 0.2 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 8 0.2 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 8 0.2 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 8 0.2 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 8 0.2 No Hit TACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGG 8 0.2 No Hit ATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTG 8 0.2 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 7 0.17500000000000002 No Hit CTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCG 7 0.17500000000000002 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 7 0.17500000000000002 No Hit CTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTC 7 0.17500000000000002 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 7 0.17500000000000002 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 7 0.17500000000000002 No Hit CGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCC 7 0.17500000000000002 No Hit GACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCC 7 0.17500000000000002 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 7 0.17500000000000002 No Hit CGTTGCCGTAGCGCTGCGGGCCTGGTCTGGGTGTGCTACTGATGGCCCGC 7 0.17500000000000002 No Hit ATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGATGGTATTC 7 0.17500000000000002 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 7 0.17500000000000002 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 7 0.17500000000000002 No Hit CTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTAC 7 0.17500000000000002 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 7 0.17500000000000002 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 7 0.17500000000000002 No Hit AAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATC 6 0.15 No Hit CTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGT 6 0.15 No Hit TGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGA 6 0.15 No Hit GGACGTTGCCGTAGCGCTGCGGGCCTGGTCTGGGTGTGCTACTGATGGCC 6 0.15 No Hit GTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAA 6 0.15 No Hit CGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGGCC 6 0.15 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 6 0.15 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 6 0.15 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 6 0.15 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 6 0.15 No Hit ATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTT 6 0.15 No Hit ATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATT 6 0.15 No Hit GTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGA 6 0.15 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 6 0.15 No Hit CTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGTTCG 5 0.125 No Hit CCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGG 5 0.125 No Hit TATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATA 5 0.125 No Hit ATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGG 5 0.125 No Hit GGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTAT 5 0.125 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 5 0.125 No Hit TTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGA 5 0.125 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 5 0.125 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 5 0.125 No Hit AGCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTG 5 0.125 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 5 0.125 No Hit CACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTT 5 0.125 No Hit CCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGA 5 0.125 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 5 0.125 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 5 0.125 No Hit AAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAA 5 0.125 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 5 0.125 No Hit TAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAG 5 0.125 No Hit AATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATG 5 0.125 No Hit TTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGA 5 0.125 No Hit CGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTG 5 0.125 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 5 0.125 No Hit GGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACA 5 0.125 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 5 0.125 No Hit AGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTC 5 0.125 No Hit GAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.05 0.0 0.0 0.0 0.0 84-85 0.05 0.0 0.0 0.0 0.0 86-87 0.05 0.0 0.0 0.0 0.0 88-89 0.05 0.0 0.0 0.0 0.0 90-91 0.075 0.0 0.0 0.0 0.0 92-93 0.075 0.0 0.0 0.0 0.0 94-95 0.075 0.0 0.0 0.0 0.0 96-97 0.075 0.0 0.0 0.0 0.0 98-99 0.075 0.0 0.0 0.0 0.0 100-101 0.1 0.0 0.0 0.0 0.0 102-103 0.1 0.0 0.0 0.0 0.0 104-105 0.1 0.0 0.0 0.0 0.0 106-107 0.125 0.0 0.0 0.0 0.0 108-109 0.125 0.0 0.0 0.0 0.0 110-111 0.125 0.0 0.0 0.0 0.0 112-113 0.125 0.0 0.0 0.0 0.0 114-115 0.125 0.0 0.0 0.0 0.0 116-117 0.15 0.0 0.0 0.0 0.0 118-119 0.15 0.0 0.0 0.0 0.0 120-121 0.15 0.0 0.0 0.0 0.0 122-123 0.175 0.0 0.0 0.0 0.0 124-125 0.2125 0.0 0.0 0.0 0.0 126-127 0.275 0.0 0.0 0.0 0.0 128-129 0.3125 0.0 0.0 0.0 0.0 130-131 0.325 0.0 0.0 0.0 0.0 132-133 0.3625 0.0 0.0 0.0 0.0 134-135 0.4 0.0 0.0 0.0 0.0 136-137 0.55 0.0 0.0 0.0 0.0 138 0.575 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAAACTG 10 0.0069754543 143.9875 4 GCTTGTA 10 0.0069754543 143.9875 5 >>END_MODULE Read 2873674 spots for ERR11006584.sra Written 2873674 spots for ERR11006584.sra Read 2873674 spots for ERR11006584.sra Written 2873674 spots for ERR11006584.sra Read 2873674 spots for ERR11006584.sra Written 2873674 spots for ERR11006584.sra Read 2873674 spots for ERR11006584.sra Written 2873674 spots for ERR11006584.sra Read 2873674 spots for ERR11006584.sra Written 2873674 spots for ERR11006584.sra Read 2873686 spots for ERR11006584.sra Written 2873686 spots for ERR11006584.sra Read 2873674 spots for ERR11006584.sra Written 2873674 spots for ERR11006584.sra Read 2873674 spots for ERR11006584.sra Written 2873674 spots for ERR11006584.sra Read 2873674 spots for ERR11006584.sra Written 2873674 spots for ERR11006584.sra Read 2873674 spots for ERR11006584.sra Written 2873674 spots for ERR11006584.sra Read 2873674 spots for ERR11006584.sra Written 2873674 spots for ERR11006584.sra Read 2873674 spots for ERR11006584.sra Read 2873674 spots for ERR11006584.sra Written 2873674 spots for ERR11006584.sra Written 2873674 spots for ERR11006584.sra Read 2873674 spots for ERR11006584.sra Written 2873674 spots for ERR11006584.sra Read 2873674 spots for ERR11006584.sra Written 2873674 spots for ERR11006584.sra Read 2873674 spots for ERR11006584.sra Written 2873674 spots for ERR11006584.sra Read 2873674 spots for ERR11006584.sra Written 2873674 spots for ERR11006584.sra Read 2873674 spots for ERR11006584.sra Written 2873674 spots for ERR11006584.sra Read 2873674 spots for ERR11006584.sra Written 2873674 spots for ERR11006584.sra Read 2873674 spots for ERR11006584.sra Written 2873674 spots for ERR11006584.sra SRR ids: ['ERR11006584.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_alktqa6n ERR11006584.sra spots: 57473492 blocks: [[1, 2873674], [2873675, 5747348], [5747349, 8621022], [8621023, 11494696], [11494697, 14368370], [14368371, 17242044], [17242045, 20115718], [20115719, 22989392], [22989393, 25863066], [25863067, 28736740], [28736741, 31610414], [31610415, 34484088], [34484089, 37357762], [37357763, 40231436], [40231437, 43105110], [43105111, 45978784], [45978785, 48852458], [48852459, 51726132], [51726133, 54599806], [54599807, 57473492]] ERR11006584 file size 21118862 ERR11006584 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006584 ERR11006584_1.fastq ERR11006584_2.fastq Input file: ERR11006584_1.fastq Paired file: ERR11006584_2.fastq trimmed: ERR11006584-trimmed-pair1.fastq, ERR11006584-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 18:21:40 2024 >> started Fri Dec 6 18:24:20 2024 >> done (160.347s) 57473492 read pairs processed; of these: 255 ( 0.00%) short read pairs filtered out after trimming by size control 3446 ( 0.01%) empty read pairs filtered out after trimming by size control 57469791 (99.99%) read pairs available; of these: 421962 ( 0.73%) trimmed read pairs available after processing 57047829 (99.27%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 12 0.00% 19 13 0.00% 20 7 0.00% 21 15 0.00% 22 27 0.00% 23 18 0.00% 24 7 0.00% 25 19 0.00% 26 17 0.00% 27 24 0.00% 28 22 0.00% 29 42 0.00% 30 19 0.00% 31 122 0.00% 32 7 0.00% 33 21 0.00% 34 21 0.00% 35 31 0.00% 36 17 0.00% 37 25 0.00% 38 34 0.00% 39 24 0.00% 40 20 0.00% 41 50 0.00% 42 31 0.00% 43 26 0.00% 44 36 0.00% 45 37 0.00% 46 37 0.00% 47 43 0.00% 48 46 0.00% 49 62 0.00% 50 45 0.00% 51 79 0.00% 52 78 0.00% 53 85 0.00% 54 74 0.00% 55 71 0.00% 56 99 0.00% 57 84 0.00% 58 98 0.00% 59 104 0.00% 60 129 0.00% 61 148 0.00% 62 155 0.00% 63 168 0.00% 64 165 0.00% 65 178 0.00% 66 177 0.00% 67 212 0.00% 68 238 0.00% 69 206 0.00% 70 202 0.00% 71 272 0.00% 72 301 0.00% 73 338 0.00% 74 308 0.00% 75 353 0.00% 76 336 0.00% 77 413 0.00% 78 402 0.00% 79 447 0.00% 80 487 0.00% 81 534 0.00% 82 574 0.00% 83 634 0.00% 84 736 0.00% 85 788 0.00% 86 745 0.00% 87 875 0.00% 88 886 0.00% 89 1005 0.00% 90 1015 0.00% 91 1065 0.00% 92 1112 0.00% 93 1256 0.00% 94 1206 0.00% 95 1367 0.00% 96 1379 0.00% 97 1616 0.00% 98 1738 0.00% 99 1703 0.00% 100 1782 0.00% 101 1964 0.00% 102 2128 0.00% 103 2216 0.00% 104 2310 0.00% 105 2485 0.00% 106 2589 0.00% 107 2705 0.00% 108 2849 0.00% 109 2974 0.01% 110 3194 0.01% 111 3400 0.01% 112 3658 0.01% 113 3685 0.01% 114 3951 0.01% 115 4142 0.01% 116 4497 0.01% 117 4812 0.01% 118 4836 0.01% 119 5283 0.01% 120 5540 0.01% 121 5905 0.01% 122 6166 0.01% 123 6441 0.01% 124 6733 0.01% 125 7393 0.01% 126 6952 0.01% 127 6953 0.01% 128 7246 0.01% 129 7963 0.01% 130 8558 0.01% 131 9191 0.02% 132 9460 0.02% 133 9669 0.02% 134 9656 0.02% 135 9781 0.02% 136 10101 0.02% 137 10835 0.02% 138 11303 0.02% 139 11983 0.02% 140 12244 0.02% 141 13210 0.02% 142 14103 0.02% 143 14717 0.03% 144 14912 0.03% 145 15987 0.03% 146 19038 0.03% 147 18170 0.03% 148 18791 0.03% 149 19883 0.03% 150 57047829 99.27% 57469791 reads passed initial QC criterion=sequence-density sequence-density=0.46 sequence-density-rank=1 fanout-score=0.00 fanout-score-rank=32 prefix-density=0.00 prefix-fanout=1.0 sequence=GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTTATCCTTCCACCGTTGGAAGCGGGCAGTTGTCGCTGCTCTGTGAAGCCAGCCTCACGCTGTGCCTGCCAACATTATGGGCCGCGAAGCCTAGCTTTCGCTTAAGCTCCAACGGCCCACTACGCAACTTGGAACGGGCGGGCCATCAGTAGCACACCCAGACCAGGCCCGCAGCGCTACGGCAACGTCCACACCACCCTTAAAGCCCCCACT criterion=fanout-score sequence-density=0.07 sequence-density-rank=21 fanout-score=67.98 fanout-score-rank=1 prefix-density=4.63 prefix-fanout=1.0 sequence=AAGCGGGCAGTGGTCGCTGCTCT criterion=sequence-density sequence-density=0.36 sequence-density-rank=1 fanout-score=13.64 fanout-score-rank=14 prefix-density=4.87 prefix-fanout=1.0 sequence=TTGCGTAGTGGATCTGCTGGGGCCTATGCGAAAGCTGGGCCTCACGAATTCTATAGTGGCAGGCACCGCGTTAGGCTGGCTTC criterion=fanout-score sequence-density=0.07 sequence-density-rank=24 fanout-score=145.26 fanout-score-rank=1 prefix-density=9.53 prefix-fanout=1.0 sequence=ATAACTAGCACAGAAAATCGTCT ERR11006584 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 18:25:14 Started mapping on | Dec 06 18:25:14 Finished on | Dec 06 18:33:13 Mapping speed, Million of reads per hour | 431.92 Number of input reads | 57469791 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 46899156 Uniquely mapped reads % | 81.61% Average mapped length | 298.50 Number of splices: Total | 18749412 Number of splices: Annotated (sjdb) | 17615895 Number of splices: GT/AG | 18393199 Number of splices: GC/AG | 213584 Number of splices: AT/AC | 20492 Number of splices: Non-canonical | 122137 Mismatch rate per base, % | 0.30% Deletion rate per base | 0.02% Deletion average length | 1.74 Insertion rate per base | 0.01% Insertion average length | 2.10 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 8491266 % of reads mapped to multiple loci | 14.78% Number of reads mapped to too many loci | 13716 % of reads mapped to too many loci | 0.02% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.98% % of reads unmapped: other | 0.62% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2079369 2079369 2079369 N_multimapping 8491266 8491266 8491266 N_noFeature 10750079 43519723 13357191 N_ambiguous 1201587 32821 427014 UnstrandedReadsAssigned:34947490 PositiveStrandReadsAssigned:3346612 NegativeStrandReadsAssigned:33114951 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006584 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006584-trimmed-pair1.fastq ERR11006584-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 57,469,791 reads, 32,963,462 reads pseudoaligned [quant] estimated average fragment length: 274.512 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,156 rounds 52973 ERR11006584.ke.tsv 35125 ERR11006584.se.tsv 88098 total ==> ERR11006584.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 663.025 0 0 PNS24247 1044 770.488 45.9306 1.8834 PNS24249 1928 1654.49 138.222 2.6395 PNS24246 1044 770.488 45.9306 1.8834 PNS24248 1044 770.488 45.9306 1.8834 PNS24244 1471 1197.49 123.986 3.27121 PNS24243 293 51.2318 0 0 KQK14069 1603 1329.49 542.848 12.9004 KQK14071 474 203.837 12.2286 1.8954 ==> ERR11006584.se.tsv <== BRADI_1g14170v3 610 BRADI_1g53295v3 216 BRADI_1g59795v3 310 BRADI_1g07683v3 0 BRADI_1g00485v3 4 BRADI_1g20270v3 1118 BRADI_1g74790v3 565 BRADI_1g09890v3 0 BRADI_1g77505v3 289 BRADI_1g48960v3 1 ERR11006584 completed mapping pipeline successfully