Starting /dee2/code/volunteer_pipeline.sh ERR11006585 current disk space = 1550496473088 free memory = 1600173584 ERR11006585 SRAfilesize 9a06e81427492b5f7175398b0386a990 ERR11006585.sra ERR11006585.sra file validated ERR11006585 is paired end ERR11006585 is conventional basespace ERR11006585 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006585_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.3055 37.0 37.0 37.0 37.0 37.0 2 36.158 37.0 37.0 37.0 37.0 37.0 3 36.2685 37.0 37.0 37.0 37.0 37.0 4 36.312 37.0 37.0 37.0 37.0 37.0 5 36.289 37.0 37.0 37.0 37.0 37.0 6 36.4305 37.0 37.0 37.0 37.0 37.0 7 36.31 37.0 37.0 37.0 37.0 37.0 8 36.3325 37.0 37.0 37.0 37.0 37.0 9 36.401 37.0 37.0 37.0 37.0 37.0 10-14 36.3788 37.0 37.0 37.0 37.0 37.0 15-19 36.3935 37.0 37.0 37.0 37.0 37.0 20-24 36.3442 37.0 37.0 37.0 37.0 37.0 25-29 36.299699999999994 37.0 37.0 37.0 37.0 37.0 30-34 36.2116 37.0 37.0 37.0 37.0 37.0 35-39 36.2125 37.0 37.0 37.0 37.0 37.0 40-44 36.179700000000004 37.0 37.0 37.0 37.0 37.0 45-49 36.166 37.0 37.0 37.0 37.0 37.0 50-54 36.1452 37.0 37.0 37.0 37.0 37.0 55-59 36.0995 37.0 37.0 37.0 37.0 37.0 60-64 36.0637 37.0 37.0 37.0 37.0 37.0 65-69 36.0379 37.0 37.0 37.0 37.0 37.0 70-74 36.0619 37.0 37.0 37.0 37.0 37.0 75-79 36.0069 37.0 37.0 37.0 37.0 37.0 80-84 35.965199999999996 37.0 37.0 37.0 37.0 37.0 85-89 35.950900000000004 37.0 37.0 37.0 37.0 37.0 90-94 35.825900000000004 37.0 37.0 37.0 37.0 37.0 95-99 35.9588 37.0 37.0 37.0 37.0 37.0 100-104 35.8811 37.0 37.0 37.0 37.0 37.0 105-109 35.8084 37.0 37.0 37.0 37.0 37.0 110-114 35.652699999999996 37.0 37.0 37.0 37.0 37.0 115-119 35.6558 37.0 37.0 37.0 37.0 37.0 120-124 35.718999999999994 37.0 37.0 37.0 37.0 37.0 125-129 35.5721 37.0 37.0 37.0 37.0 37.0 130-134 35.4994 37.0 37.0 37.0 37.0 37.0 135-139 35.342 37.0 37.0 37.0 34.6 37.0 140-144 35.1879 37.0 37.0 37.0 27.4 37.0 145-149 35.327099999999994 37.0 37.0 37.0 34.6 37.0 150 35.058 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 1.0 18 0.0 19 1.0 20 0.0 21 0.0 22 2.0 23 1.0 24 2.0 25 3.0 26 10.0 27 15.0 28 17.0 29 38.0 30 36.0 31 82.0 32 75.0 33 113.0 34 168.0 35 380.0 36 2819.0 37 236.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.95 13.325000000000001 16.075 32.65 2 29.475 10.95 26.025 33.550000000000004 3 23.400000000000002 14.424999999999999 26.85 35.325 4 27.700000000000003 15.825 26.025 30.45 5 28.95 18.35 26.974999999999998 25.724999999999998 6 24.75 27.3 21.425 26.525 7 16.175 30.125 34.275 19.425 8 17.275 29.875 33.125 19.725 9 18.175 26.875 35.699999999999996 19.25 10-14 19.919999999999998 31.59 26.590000000000003 21.9 15-19 20.885 34.005 24.26 20.849999999999998 20-24 19.325 29.15 28.205000000000002 23.32 25-29 22.384999999999998 31.41 24.795 21.41 30-34 23.275000000000002 30.580000000000002 24.490000000000002 21.654999999999998 35-39 23.385 31.795 24.115000000000002 20.705000000000002 40-44 21.279999999999998 30.769999999999996 24.615000000000002 23.335 45-49 20.685000000000002 29.56 26.39 23.365 50-54 21.21 32.57 25.324999999999996 20.895 55-59 21.73 29.82 23.685000000000002 24.765 60-64 20.68 30.875000000000004 25.245 23.200000000000003 65-69 21.560000000000002 29.849999999999998 24.635 23.955000000000002 70-74 24.05 29.13 22.11 24.709999999999997 75-79 22.98 29.975 25.224999999999998 21.82 80-84 22.97 29.17 24.104999999999997 23.755000000000003 85-89 24.32 28.685 24.93 22.065 90-94 22.185 29.604999999999997 25.779999999999998 22.43 95-99 24.15 28.955 23.585 23.31 100-104 21.625 31.05 23.185 24.14 105-109 21.95 28.494999999999997 25.44 24.115000000000002 110-114 23.07 28.060000000000002 25.39 23.48 115-119 20.75 29.92 25.624999999999996 23.705000000000002 120-124 19.475 30.264999999999997 24.64 25.619999999999997 125-129 20.185 32.015 22.275 25.525 130-134 22.745 30.285 24.95 22.02 135-139 23.87 29.705 22.16 24.265 140-144 24.095 29.080000000000002 25.919999999999998 20.905 145-149 23.34 30.104999999999997 24.47 22.085 150 24.4 27.150000000000002 26.875 21.575 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.5 20 1.5 21 1.5 22 2.0 23 2.0 24 3.0 25 4.0 26 3.5 27 4.5 28 6.5 29 15.0 30 19.0 31 20.0 32 28.5 33 31.5 34 38.0 35 48.0 36 75.0 37 161.5 38 294.5 39 291.0 40 245.0 41 282.0 42 252.0 43 223.5 44 224.5 45 221.0 46 196.5 47 152.0 48 123.0 49 94.0 50 73.5 51 60.0 52 57.0 53 46.5 54 35.5 55 33.0 56 29.5 57 26.5 58 22.5 59 23.5 60 25.5 61 27.0 62 25.0 63 25.5 64 64.5 65 101.5 66 58.0 67 19.5 68 22.0 69 19.0 70 18.5 71 18.0 72 16.0 73 15.5 74 15.5 75 12.5 76 8.0 77 7.5 78 10.5 79 6.5 80 2.0 81 1.5 82 2.5 83 3.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.225 #Duplication Level Percentage of deduplicated Percentage of total 1 80.80965356169716 51.9 2 12.105877773452706 15.55 3 2.958349552355002 5.7 4 0.973141300116777 2.5 5 0.7785130400934216 2.5 6 0.3503308680420397 1.35 7 0.42818217205138187 1.925 8 0.2724795640326975 1.4000000000000001 9 0.1946282600233554 1.125 >10 1.08991825613079 12.55 >50 0.0 0.0 >100 0.038925652004671074 3.5000000000000004 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 140 3.5000000000000004 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 36 0.8999999999999999 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 35 0.8750000000000001 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 31 0.775 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 30 0.75 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 27 0.675 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 26 0.65 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 23 0.575 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 23 0.575 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 23 0.575 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 22 0.5499999999999999 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 21 0.525 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 18 0.44999999999999996 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 15 0.375 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 15 0.375 No Hit CACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAAT 15 0.375 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 13 0.325 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 12 0.3 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 12 0.3 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 12 0.3 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 11 0.27499999999999997 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 11 0.27499999999999997 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 11 0.27499999999999997 No Hit TCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCG 10 0.25 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 10 0.25 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 10 0.25 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 10 0.25 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 10 0.25 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 10 0.25 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 9 0.22499999999999998 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 9 0.22499999999999998 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 9 0.22499999999999998 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 9 0.22499999999999998 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 9 0.22499999999999998 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 8 0.2 No Hit GCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCC 8 0.2 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 8 0.2 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 8 0.2 No Hit GGCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGAT 8 0.2 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 8 0.2 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 8 0.2 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 7 0.17500000000000002 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 7 0.17500000000000002 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 7 0.17500000000000002 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 7 0.17500000000000002 No Hit TAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTA 7 0.17500000000000002 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 7 0.17500000000000002 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 7 0.17500000000000002 No Hit GTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCG 7 0.17500000000000002 No Hit TAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGA 7 0.17500000000000002 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 7 0.17500000000000002 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 7 0.17500000000000002 No Hit TGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 6 0.15 No Hit ACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 6 0.15 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 6 0.15 No Hit CTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAG 6 0.15 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 6 0.15 No Hit TCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATC 6 0.15 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 6 0.15 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 6 0.15 No Hit GGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGG 6 0.15 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 5 0.125 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 5 0.125 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 5 0.125 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 5 0.125 No Hit TCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGT 5 0.125 No Hit CCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGC 5 0.125 No Hit CCATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGT 5 0.125 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 5 0.125 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 5 0.125 No Hit ATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGA 5 0.125 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 5 0.125 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 5 0.125 No Hit GGTGGTTCTTTGGAGTAGGATATGAGACCCAAGCGGGCCGGGAATGCAGC 5 0.125 No Hit GCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAGCTGCAA 5 0.125 No Hit AACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAG 5 0.125 No Hit GTTCCCACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGA 5 0.125 No Hit ACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAG 5 0.125 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 5 0.125 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 5 0.125 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0125 12-13 0.0 0.0 0.0 0.0 0.025 14-15 0.0 0.0 0.0 0.0 0.025 16-17 0.0 0.0 0.0 0.0 0.025 18-19 0.0 0.0 0.0 0.0 0.025 20-21 0.0 0.0 0.0 0.0 0.025 22-23 0.0 0.0 0.0 0.0 0.025 24-25 0.0 0.0 0.0 0.0 0.025 26-27 0.0 0.0 0.0 0.0 0.025 28-29 0.0 0.0 0.0 0.0 0.025 30-31 0.0 0.0 0.0 0.0 0.025 32-33 0.0 0.0 0.0 0.0 0.025 34-35 0.0 0.0 0.0 0.0 0.025 36-37 0.0 0.0 0.0 0.0 0.025 38-39 0.0 0.0 0.0 0.0 0.025 40-41 0.0 0.0 0.0 0.0 0.025 42-43 0.0 0.0 0.0 0.0 0.025 44-45 0.0 0.0 0.0 0.0 0.025 46-47 0.0 0.0 0.0 0.0 0.025 48-49 0.0 0.0 0.0 0.0 0.025 50-51 0.0 0.0 0.0 0.0 0.025 52-53 0.0 0.0 0.0 0.0 0.025 54-55 0.0 0.0 0.0 0.0 0.025 56-57 0.0 0.0 0.0 0.0 0.025 58-59 0.0 0.0 0.0 0.0 0.025 60-61 0.0 0.0 0.0 0.0 0.025 62-63 0.0 0.0 0.0 0.0 0.025 64-65 0.0 0.0 0.0 0.0 0.025 66-67 0.0 0.0 0.0 0.0 0.025 68-69 0.0 0.0 0.0 0.0 0.025 70-71 0.0 0.0 0.0 0.0 0.025 72-73 0.0 0.0 0.0 0.0 0.025 74-75 0.0 0.0 0.0 0.0 0.025 76-77 0.0 0.0 0.0 0.0 0.025 78-79 0.0 0.0 0.0 0.0 0.025 80-81 0.0 0.0 0.0 0.0 0.025 82-83 0.0 0.0 0.0 0.0 0.025 84-85 0.0 0.0 0.0 0.0 0.025 86-87 0.0 0.0 0.0 0.0 0.025 88-89 0.0 0.0 0.0 0.0 0.025 90-91 0.0 0.0 0.0 0.0 0.025 92-93 0.0 0.0 0.0 0.0 0.025 94-95 0.0 0.0 0.0 0.0 0.025 96-97 0.0 0.0 0.0 0.0 0.025 98-99 0.0 0.0 0.0 0.0 0.025 100-101 0.0125 0.0 0.0 0.0 0.025 102-103 0.05 0.0 0.0 0.0 0.025 104-105 0.05 0.0 0.0 0.0 0.025 106-107 0.075 0.0 0.0 0.0 0.025 108-109 0.1 0.0 0.0 0.0 0.025 110-111 0.1125 0.0 0.0 0.0 0.025 112-113 0.125 0.0 0.0 0.0 0.025 114-115 0.125 0.0 0.0 0.0 0.025 116-117 0.125 0.0 0.0 0.0 0.025 118-119 0.16249999999999998 0.0 0.0 0.0 0.025 120-121 0.175 0.0 0.0 0.0 0.025 122-123 0.175 0.0 0.0 0.0 0.025 124-125 0.2 0.0 0.0 0.0 0.025 126-127 0.2 0.0 0.0 0.0 0.025 128-129 0.2 0.0 0.0 0.0 0.025 130-131 0.2 0.0 0.0 0.0 0.025 132-133 0.225 0.0 0.0 0.0 0.025 134-135 0.25 0.0 0.0 0.0 0.025 136-137 0.25 0.0 0.0 0.0 0.025 138 0.25 0.0 0.0 0.0 0.025 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGGTGG 10 0.006973645 144.0 1 GCTTTCT 20 3.687869E-4 108.0 1 TCTTTTC 30 0.0018473949 72.0 5 TTTCTTT 35 0.0034045284 61.714283 3 TTTCTTC 35 0.0034045284 61.714283 8 TTTTCTT 35 0.0034045284 61.714283 7 TTCTTTT 35 0.0034045284 61.714283 4 TTCTTCA 35 0.0034045284 61.714283 9 CTTTCTT 35 0.0034045284 61.714283 2 CTTTTCT 40 0.005777437 54.0 6 TAGCGGA 30 4.3922482E-5 28.8 30-34 TGCATTA 30 4.3922482E-5 28.8 110-114 TCAAGAG 30 4.3922482E-5 28.8 65-69 TATGTTA 30 4.3922482E-5 28.8 25-29 AGGGAAG 30 4.3922482E-5 28.8 85-89 ATTACGT 30 4.3922482E-5 28.8 100-104 AGCGGGA 25 5.183459E-4 28.8 55-59 AGTTGTG 30 4.3922482E-5 28.8 90-94 ACCTTAT 30 4.3922482E-5 28.8 40-44 CTAGATC 30 4.3922482E-5 28.8 75-79 >>END_MODULE ERR11006585 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006585_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.7375 37.0 37.0 37.0 37.0 37.0 2 36.146 37.0 37.0 37.0 37.0 37.0 3 36.186 37.0 37.0 37.0 37.0 37.0 4 36.2425 37.0 37.0 37.0 37.0 37.0 5 36.3025 37.0 37.0 37.0 37.0 37.0 6 36.194 37.0 37.0 37.0 37.0 37.0 7 36.2035 37.0 37.0 37.0 37.0 37.0 8 36.3675 37.0 37.0 37.0 37.0 37.0 9 36.152 37.0 37.0 37.0 37.0 37.0 10-14 36.2211 37.0 37.0 37.0 37.0 37.0 15-19 36.2922 37.0 37.0 37.0 37.0 37.0 20-24 36.167899999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.1596 37.0 37.0 37.0 37.0 37.0 30-34 36.1084 37.0 37.0 37.0 37.0 37.0 35-39 36.145 37.0 37.0 37.0 37.0 37.0 40-44 36.1149 37.0 37.0 37.0 37.0 37.0 45-49 36.0194 37.0 37.0 37.0 37.0 37.0 50-54 36.050599999999996 37.0 37.0 37.0 37.0 37.0 55-59 36.0038 37.0 37.0 37.0 37.0 37.0 60-64 35.9061 37.0 37.0 37.0 37.0 37.0 65-69 35.915299999999995 37.0 37.0 37.0 37.0 37.0 70-74 35.8241 37.0 37.0 37.0 37.0 37.0 75-79 35.830200000000005 37.0 37.0 37.0 37.0 37.0 80-84 35.8428 37.0 37.0 37.0 37.0 37.0 85-89 35.7161 37.0 37.0 37.0 37.0 37.0 90-94 35.773 37.0 37.0 37.0 37.0 37.0 95-99 35.56079999999999 37.0 37.0 37.0 37.0 37.0 100-104 35.5653 37.0 37.0 37.0 37.0 37.0 105-109 35.4282 37.0 37.0 37.0 37.0 37.0 110-114 35.3512 37.0 37.0 37.0 37.0 37.0 115-119 35.362199999999994 37.0 37.0 37.0 34.6 37.0 120-124 35.2002 37.0 37.0 37.0 27.4 37.0 125-129 35.235400000000006 37.0 37.0 37.0 27.4 37.0 130-134 35.1143 37.0 37.0 37.0 25.0 37.0 135-139 35.0453 37.0 37.0 37.0 25.0 37.0 140-144 34.984300000000005 37.0 37.0 37.0 25.0 37.0 145-149 35.075599999999994 37.0 37.0 37.0 25.0 37.0 150 34.6905 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 0.0 17 0.0 18 2.0 19 1.0 20 0.0 21 0.0 22 1.0 23 8.0 24 6.0 25 6.0 26 8.0 27 17.0 28 22.0 29 28.0 30 44.0 31 59.0 32 84.0 33 128.0 34 221.0 35 635.0 36 2584.0 37 145.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.498731608320647 25.596144089294775 18.949771689497716 27.95535261288686 2 25.650000000000002 24.775 31.775 17.8 3 20.825 26.125 33.650000000000006 19.400000000000002 4 24.099999999999998 27.625 28.275 20.0 5 23.875 28.849999999999998 28.199999999999996 19.075 6 20.200000000000003 31.874999999999996 28.349999999999998 19.575 7 20.424999999999997 20.849999999999998 40.65 18.075 8 23.75 21.825 30.9 23.525 9 22.375 20.150000000000002 33.975 23.5 10-14 23.605 26.22 28.835 21.34 15-19 24.825 24.035 30.455 20.685000000000002 20-24 25.0 23.605 30.635 20.76 25-29 25.319999999999997 23.674999999999997 29.970000000000002 21.035 30-34 24.990000000000002 24.39 29.99 20.630000000000003 35-39 24.07 24.005000000000003 30.214999999999996 21.709999999999997 40-44 23.025000000000002 24.875 29.86 22.24 45-49 23.599999999999998 25.755 29.48 21.165 50-54 23.75 25.455 28.98 21.815 55-59 23.674999999999997 24.86 28.265 23.200000000000003 60-64 23.72 24.33 29.425 22.525000000000002 65-69 24.595 24.29 29.015 22.1 70-74 24.335 24.67 29.775000000000002 21.22 75-79 24.455 24.044999999999998 30.305 21.195 80-84 25.385 23.945 29.69 20.979999999999997 85-89 24.205 25.180000000000003 27.785 22.830000000000002 90-94 23.57 24.060000000000002 29.294999999999998 23.075000000000003 95-99 23.705000000000002 24.42 29.799999999999997 22.075 100-104 24.375 24.01 29.9 21.715 105-109 26.08 23.135 29.270000000000003 21.515 110-114 24.36 23.89 29.18 22.57 115-119 24.23 25.2 28.505000000000003 22.065 120-124 24.57 24.52 28.505000000000003 22.405 125-129 22.759999999999998 25.480000000000004 29.925 21.834999999999997 130-134 22.63 26.69 28.415000000000003 22.264999999999997 135-139 23.275000000000002 25.31 29.599999999999998 21.815 140-144 23.845 25.040000000000003 29.935000000000002 21.18 145-149 22.795 23.945 30.475 22.785 150 24.15 23.25 29.95 22.650000000000002 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.0 20 0.0 21 2.0 22 2.5 23 2.5 24 7.0 25 11.5 26 12.5 27 11.0 28 11.0 29 15.0 30 23.0 31 27.0 32 28.5 33 32.0 34 51.5 35 85.0 36 119.0 37 176.0 38 209.0 39 213.0 40 237.0 41 253.0 42 237.0 43 225.5 44 232.0 45 214.5 46 189.0 47 153.0 48 116.5 49 89.5 50 68.0 51 56.5 52 51.0 53 44.0 54 33.5 55 33.0 56 25.5 57 28.0 58 31.5 59 30.0 60 32.5 61 30.0 62 33.0 63 69.5 64 87.0 65 72.0 66 47.0 67 24.0 68 24.5 69 22.5 70 23.0 71 25.0 72 22.0 73 19.0 74 19.0 75 16.5 76 11.0 77 8.0 78 7.0 79 4.5 80 5.0 81 3.5 82 1.5 83 2.0 84 2.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.4500000000000002 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.95 #Duplication Level Percentage of deduplicated Percentage of total 1 79.72774098601914 54.175 2 12.10448859455482 16.45 3 3.384841795437822 6.9 4 1.8763796909492272 5.1 5 0.7358351729212657 2.5 6 0.5518763796909493 2.25 7 0.33112582781456956 1.575 8 0.33112582781456956 1.7999999999999998 9 0.22075055187637968 1.35 >10 0.7358351729212657 7.9 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 43 1.075 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 31 0.775 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 23 0.575 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 20 0.5 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 18 0.44999999999999996 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 17 0.42500000000000004 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 15 0.375 No Hit AGCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTG 13 0.325 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 13 0.325 No Hit GCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGC 13 0.325 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 13 0.325 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 12 0.3 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 11 0.27499999999999997 No Hit GCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGC 11 0.27499999999999997 No Hit AGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAG 11 0.27499999999999997 No Hit GAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCG 11 0.27499999999999997 No Hit TGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACT 11 0.27499999999999997 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 10 0.25 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 10 0.25 No Hit AATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGC 10 0.25 No Hit CTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCG 9 0.22499999999999998 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 9 0.22499999999999998 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 9 0.22499999999999998 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 9 0.22499999999999998 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 9 0.22499999999999998 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 9 0.22499999999999998 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 8 0.2 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 8 0.2 No Hit GATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTC 8 0.2 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 8 0.2 No Hit GGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTT 8 0.2 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 8 0.2 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 8 0.2 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 8 0.2 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 8 0.2 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 7 0.17500000000000002 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 7 0.17500000000000002 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 7 0.17500000000000002 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 7 0.17500000000000002 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 7 0.17500000000000002 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 7 0.17500000000000002 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 7 0.17500000000000002 No Hit TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC 7 0.17500000000000002 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 7 0.17500000000000002 No Hit AGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTTAT 6 0.15 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 6 0.15 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 6 0.15 No Hit GTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTT 6 0.15 No Hit TGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTC 6 0.15 No Hit ATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGA 6 0.15 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 6 0.15 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 6 0.15 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 6 0.15 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 6 0.15 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 6 0.15 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 6 0.15 No Hit TCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTG 6 0.15 No Hit TGTGGACGTTGCCGTAGCGCTGCGGGCCTGGTCTGGGTGTGCTACTGATG 6 0.15 No Hit GAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGT 6 0.15 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 5 0.125 No Hit ATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGC 5 0.125 No Hit GAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTT 5 0.125 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 5 0.125 No Hit CCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTG 5 0.125 No Hit GTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACC 5 0.125 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 5 0.125 No Hit CATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGA 5 0.125 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 5 0.125 No Hit GGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCG 5 0.125 No Hit GAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTC 5 0.125 No Hit AACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGG 5 0.125 No Hit CTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAG 5 0.125 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 5 0.125 No Hit CTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTTATGA 5 0.125 No Hit TTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGG 5 0.125 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 5 0.125 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 5 0.125 No Hit CATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGGCG 5 0.125 No Hit GTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0125 0.0 0.0 0.0 0.0 102-103 0.05 0.0 0.0 0.0 0.0 104-105 0.05 0.0 0.0 0.0 0.0 106-107 0.075 0.0 0.0 0.0 0.0 108-109 0.1 0.0 0.0 0.0 0.0 110-111 0.1125 0.0 0.0 0.0 0.0 112-113 0.125 0.0 0.0 0.0 0.0 114-115 0.125 0.0 0.0 0.0 0.0 116-117 0.125 0.0 0.0 0.0 0.0 118-119 0.16249999999999998 0.0 0.0 0.0 0.0 120-121 0.175 0.0 0.0 0.0 0.0 122-123 0.175 0.0 0.0 0.0 0.0 124-125 0.2 0.0 0.0 0.0 0.0 126-127 0.2 0.0 0.0 0.0 0.0 128-129 0.2 0.0 0.0 0.0 0.0 130-131 0.2 0.0 0.0 0.0 0.0 132-133 0.225 0.0 0.0 0.0 0.0 134-135 0.225 0.0 0.0 0.0 0.0 136-137 0.225 0.0 0.0 0.0 0.0 138 0.225 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCTTTC 10 0.0069754543 143.9875 3 >>END_MODULE Read 2698922 spots for ERR11006585.sra Written 2698922 spots for ERR11006585.sra Read 2698922 spots for ERR11006585.sra Written 2698922 spots for ERR11006585.sra Read 2698922 spots for ERR11006585.sra Written 2698922 spots for ERR11006585.sra Read 2698922 spots for ERR11006585.sra Written 2698922 spots for ERR11006585.sra Read 2698922 spots for ERR11006585.sra Written 2698922 spots for ERR11006585.sra Read 2698922 spots for ERR11006585.sra Written 2698922 spots for ERR11006585.sra Read 2698922 spots for ERR11006585.sra Written 2698922 spots for ERR11006585.sra Read 2698922 spots for ERR11006585.sra Written 2698922 spots for ERR11006585.sra Read 2698922 spots for ERR11006585.sra Written 2698922 spots for ERR11006585.sra Read 2698922 spots for ERR11006585.sra Written 2698922 spots for ERR11006585.sra Read 2698922 spots for ERR11006585.sra Written 2698922 spots for ERR11006585.sra Read 2698922 spots for ERR11006585.sra Written 2698922 spots for ERR11006585.sra Read 2698922 spots for ERR11006585.sra Written 2698922 spots for ERR11006585.sra Read 2698926 spots for ERR11006585.sra Written 2698926 spots for ERR11006585.sra Read 2698922 spots for ERR11006585.sra Written 2698922 spots for ERR11006585.sra Read 2698922 spots for ERR11006585.sra Written 2698922 spots for ERR11006585.sra Read 2698922 spots for ERR11006585.sra Written 2698922 spots for ERR11006585.sra Read 2698922 spots for ERR11006585.sra Written 2698922 spots for ERR11006585.sra Read 2698922 spots for ERR11006585.sra Written 2698922 spots for ERR11006585.sra Read 2698922 spots for ERR11006585.sra Written 2698922 spots for ERR11006585.sra SRR ids: ['ERR11006585.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_s0tdcgfe ERR11006585.sra spots: 53978444 blocks: [[1, 2698922], [2698923, 5397844], [5397845, 8096766], [8096767, 10795688], [10795689, 13494610], [13494611, 16193532], [16193533, 18892454], [18892455, 21591376], [21591377, 24290298], [24290299, 26989220], [26989221, 29688142], [29688143, 32387064], [32387065, 35085986], [35085987, 37784908], [37784909, 40483830], [40483831, 43182752], [43182753, 45881674], [45881675, 48580596], [48580597, 51279518], [51279519, 53978444]] ERR11006585 file size 19833955 ERR11006585 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006585 ERR11006585_1.fastq ERR11006585_2.fastq Input file: ERR11006585_1.fastq Paired file: ERR11006585_2.fastq trimmed: ERR11006585-trimmed-pair1.fastq, ERR11006585-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 18:21:41 2024 >> started Fri Dec 6 18:23:27 2024 >> done (106.444s) 53978444 read pairs processed; of these: 221 ( 0.00%) short read pairs filtered out after trimming by size control 1657 ( 0.00%) empty read pairs filtered out after trimming by size control 53976566 (100.00%) read pairs available; of these: 297804 ( 0.55%) trimmed read pairs available after processing 53678762 (99.45%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 9 0.00% 19 11 0.00% 20 8 0.00% 21 10 0.00% 22 22 0.00% 23 10 0.00% 24 12 0.00% 25 34 0.00% 26 13 0.00% 27 21 0.00% 28 22 0.00% 29 19 0.00% 30 14 0.00% 31 110 0.00% 32 20 0.00% 33 20 0.00% 34 15 0.00% 35 22 0.00% 36 14 0.00% 37 28 0.00% 38 31 0.00% 39 28 0.00% 40 32 0.00% 41 26 0.00% 42 25 0.00% 43 34 0.00% 44 35 0.00% 45 29 0.00% 46 33 0.00% 47 41 0.00% 48 48 0.00% 49 46 0.00% 50 66 0.00% 51 62 0.00% 52 65 0.00% 53 65 0.00% 54 53 0.00% 55 58 0.00% 56 47 0.00% 57 89 0.00% 58 75 0.00% 59 89 0.00% 60 116 0.00% 61 102 0.00% 62 120 0.00% 63 129 0.00% 64 152 0.00% 65 182 0.00% 66 171 0.00% 67 183 0.00% 68 183 0.00% 69 175 0.00% 70 206 0.00% 71 234 0.00% 72 244 0.00% 73 290 0.00% 74 319 0.00% 75 346 0.00% 76 348 0.00% 77 369 0.00% 78 389 0.00% 79 414 0.00% 80 481 0.00% 81 440 0.00% 82 506 0.00% 83 527 0.00% 84 596 0.00% 85 631 0.00% 86 700 0.00% 87 829 0.00% 88 820 0.00% 89 857 0.00% 90 980 0.00% 91 894 0.00% 92 1006 0.00% 93 1021 0.00% 94 1080 0.00% 95 1190 0.00% 96 1222 0.00% 97 1298 0.00% 98 1395 0.00% 99 1504 0.00% 100 1446 0.00% 101 1534 0.00% 102 1599 0.00% 103 1792 0.00% 104 1777 0.00% 105 1931 0.00% 106 1978 0.00% 107 2092 0.00% 108 2235 0.00% 109 2331 0.00% 110 2583 0.00% 111 2623 0.00% 112 2675 0.00% 113 2938 0.01% 114 2998 0.01% 115 3147 0.01% 116 3345 0.01% 117 3511 0.01% 118 3538 0.01% 119 3712 0.01% 120 4018 0.01% 121 4179 0.01% 122 4535 0.01% 123 4629 0.01% 124 4652 0.01% 125 5341 0.01% 126 4955 0.01% 127 4726 0.01% 128 5225 0.01% 129 5540 0.01% 130 6093 0.01% 131 6387 0.01% 132 6582 0.01% 133 6401 0.01% 134 6627 0.01% 135 6700 0.01% 136 7105 0.01% 137 7225 0.01% 138 7609 0.01% 139 8127 0.02% 140 8256 0.02% 141 8824 0.02% 142 9171 0.02% 143 9611 0.02% 144 9729 0.02% 145 10784 0.02% 146 12824 0.02% 147 12178 0.02% 148 12362 0.02% 149 13464 0.02% 150 53678762 99.45% 53976566 reads passed initial QC criterion=sequence-density sequence-density=0.41 sequence-density-rank=1 fanout-score=0.00 fanout-score-rank=28 prefix-density=0.00 prefix-fanout=1.0 sequence=GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTTATCCTTCCACCGTTGGAAGCGGGCAGTTGTCGCTGCTCTGTGAAGCCAGCCTCACGCTGTGCCTGCCAACATTATGGGCCGCGAAGCCTAGCTTTCGCTTAAGCTCCAACGGCCCACTACGCAACTTGGAACGGGCGGGCCATCAGTAGCACACCCAGACCAGGCCCGCAGCGCTACGGCAACGTCCACACCACCCTTAAAGCCCCCAC criterion=fanout-score sequence-density=0.08 sequence-density-rank=17 fanout-score=55.82 fanout-score-rank=1 prefix-density=4.21 prefix-fanout=1.0 sequence=TGCTCTGTGAAACCAGCCTCACG criterion=sequence-density sequence-density=0.30 sequence-density-rank=1 fanout-score=13.96 fanout-score-rank=11 prefix-density=4.18 prefix-fanout=1.0 sequence=TTGCGTAGTGGATCTGCTGGGGCCTATGCGAAAGCTGGGCCTCACGAATTCTATAGTGGCAGGCACCGCGTTAGGCTGGCTTC criterion=fanout-score sequence-density=0.07 sequence-density-rank=19 fanout-score=145.74 fanout-score-rank=1 prefix-density=10.06 prefix-fanout=1.1 sequence=AGCACTGAAAAACGTCTTTACAT ERR11006585 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 18:25:19 Started mapping on | Dec 06 18:25:21 Finished on | Dec 06 18:31:30 Mapping speed, Million of reads per hour | 526.60 Number of input reads | 53976566 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 42931455 Uniquely mapped reads % | 79.54% Average mapped length | 298.49 Number of splices: Total | 15499203 Number of splices: Annotated (sjdb) | 14523854 Number of splices: GT/AG | 15183862 Number of splices: GC/AG | 177616 Number of splices: AT/AC | 20039 Number of splices: Non-canonical | 117686 Mismatch rate per base, % | 0.33% Deletion rate per base | 0.02% Deletion average length | 1.67 Insertion rate per base | 0.01% Insertion average length | 2.07 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 9235430 % of reads mapped to multiple loci | 17.11% Number of reads mapped to too many loci | 8800 % of reads mapped to too many loci | 0.02% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.94% % of reads unmapped: other | 0.40% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1809681 1809681 1809681 N_multimapping 9235430 9235430 9235430 N_noFeature 10483984 39629256 12946065 N_ambiguous 1323490 34295 485327 UnstrandedReadsAssigned:31123981 PositiveStrandReadsAssigned:3267904 NegativeStrandReadsAssigned:29500063 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006585 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006585-trimmed-pair1.fastq ERR11006585-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 53,976,566 reads, 30,546,932 reads pseudoaligned [quant] estimated average fragment length: 284.563 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,137 rounds 52973 ERR11006585.ke.tsv 35125 ERR11006585.se.tsv 88098 total ==> ERR11006585.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 652.983 0 0 PNS24247 1044 760.437 31.4274 1.32035 PNS24249 1928 1644.44 163.407 3.17465 PNS24246 1044 760.437 31.4274 1.32035 PNS24248 1044 760.437 31.4274 1.32035 PNS24244 1471 1187.44 66.3112 1.7841 PNS24243 293 47.4303 4 2.69431 KQK14069 1603 1319.44 848.307 20.5403 KQK14071 474 193.848 19.7043 3.24744 ==> ERR11006585.se.tsv <== BRADI_1g14170v3 961 BRADI_1g53295v3 148 BRADI_1g59795v3 269 BRADI_1g07683v3 0 BRADI_1g00485v3 9 BRADI_1g20270v3 795 BRADI_1g74790v3 380 BRADI_1g09890v3 0 BRADI_1g77505v3 240 BRADI_1g48960v3 2 ERR11006585 completed mapping pipeline successfully