Starting /dee2/code/volunteer_pipeline.sh ERR11006586 current disk space = 1550498549760 free memory = 1600496924 ERR11006586 SRAfilesize 8668850f54341b29fb9ee809e6143196 ERR11006586.sra ERR11006586.sra file validated ERR11006586 is paired end ERR11006586 is conventional basespace ERR11006586 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006586_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.3925 37.0 37.0 37.0 37.0 37.0 2 36.195 37.0 37.0 37.0 37.0 37.0 3 36.401 37.0 37.0 37.0 37.0 37.0 4 36.348 37.0 37.0 37.0 37.0 37.0 5 36.4585 37.0 37.0 37.0 37.0 37.0 6 36.436 37.0 37.0 37.0 37.0 37.0 7 36.3645 37.0 37.0 37.0 37.0 37.0 8 36.4035 37.0 37.0 37.0 37.0 37.0 9 36.4115 37.0 37.0 37.0 37.0 37.0 10-14 36.461 37.0 37.0 37.0 37.0 37.0 15-19 36.4283 37.0 37.0 37.0 37.0 37.0 20-24 36.39020000000001 37.0 37.0 37.0 37.0 37.0 25-29 36.255700000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.242200000000004 37.0 37.0 37.0 37.0 37.0 35-39 36.241200000000006 37.0 37.0 37.0 37.0 37.0 40-44 36.175 37.0 37.0 37.0 37.0 37.0 45-49 36.123 37.0 37.0 37.0 37.0 37.0 50-54 36.144600000000004 37.0 37.0 37.0 37.0 37.0 55-59 36.1843 37.0 37.0 37.0 37.0 37.0 60-64 36.088300000000004 37.0 37.0 37.0 37.0 37.0 65-69 36.131299999999996 37.0 37.0 37.0 37.0 37.0 70-74 36.1222 37.0 37.0 37.0 37.0 37.0 75-79 36.024699999999996 37.0 37.0 37.0 37.0 37.0 80-84 36.039300000000004 37.0 37.0 37.0 37.0 37.0 85-89 35.977199999999996 37.0 37.0 37.0 37.0 37.0 90-94 35.9114 37.0 37.0 37.0 37.0 37.0 95-99 35.9478 37.0 37.0 37.0 37.0 37.0 100-104 35.863 37.0 37.0 37.0 37.0 37.0 105-109 35.782999999999994 37.0 37.0 37.0 37.0 37.0 110-114 35.6469 37.0 37.0 37.0 37.0 37.0 115-119 35.6762 37.0 37.0 37.0 37.0 37.0 120-124 35.644 37.0 37.0 37.0 37.0 37.0 125-129 35.5226 37.0 37.0 37.0 37.0 37.0 130-134 35.420300000000005 37.0 37.0 37.0 37.0 37.0 135-139 35.3772 37.0 37.0 37.0 34.6 37.0 140-144 35.1472 37.0 37.0 37.0 27.4 37.0 145-149 35.294999999999995 37.0 37.0 37.0 32.2 37.0 150 35.1045 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 1.0 21 0.0 22 1.0 23 2.0 24 2.0 25 7.0 26 6.0 27 20.0 28 26.0 29 34.0 30 47.0 31 67.0 32 75.0 33 101.0 34 173.0 35 348.0 36 2810.0 37 279.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.275 13.15 14.875 33.7 2 31.1 11.0 25.474999999999998 32.425 3 23.325000000000003 13.55 25.825 37.3 4 29.025000000000002 14.725 24.25 32.0 5 30.025000000000002 18.875 24.474999999999998 26.625 6 26.900000000000002 25.900000000000002 20.849999999999998 26.35 7 19.8 28.549999999999997 33.35 18.3 8 18.725 28.375 32.65 20.25 9 20.0 27.150000000000002 34.0 18.85 10-14 21.465 30.735 26.605 21.195 15-19 22.8 30.654999999999998 24.8 21.745 20-24 21.44 28.24 26.66 23.66 25-29 22.470000000000002 30.675 25.124999999999996 21.73 30-34 22.91 29.654999999999998 25.2 22.235 35-39 23.005 30.104999999999997 25.264999999999997 21.625 40-44 21.64 28.794999999999998 26.169999999999998 23.395 45-49 21.805 28.470000000000002 26.215 23.51 50-54 20.794999999999998 30.675 25.83 22.7 55-59 21.685 29.17 24.88 24.265 60-64 20.325 29.959999999999997 26.3 23.415 65-69 21.55 29.770000000000003 25.624999999999996 23.055 70-74 23.04 29.445 23.425 24.09 75-79 22.93 28.68 25.525 22.865 80-84 23.45 28.044999999999998 25.485000000000003 23.02 85-89 23.22 27.705000000000002 26.305 22.770000000000003 90-94 20.9 28.92 27.015 23.165 95-99 23.465 28.17 24.39 23.974999999999998 100-104 22.06 30.09 24.51 23.34 105-109 22.225 28.115000000000002 26.11 23.549999999999997 110-114 22.945 27.42 25.6 24.035 115-119 20.9 29.299999999999997 25.130000000000003 24.67 120-124 20.599999999999998 29.95 25.06 24.39 125-129 21.265 30.104999999999997 23.87 24.759999999999998 130-134 22.994999999999997 29.095 24.5 23.41 135-139 23.919999999999998 29.07 23.195 23.815 140-144 23.66 28.98 25.369999999999997 21.990000000000002 145-149 22.400000000000002 29.215000000000003 25.36 23.025000000000002 150 22.575 27.800000000000004 26.575 23.05 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 1.5 21 3.0 22 4.5 23 4.5 24 5.5 25 6.0 26 9.5 27 18.5 28 18.5 29 19.5 30 29.5 31 34.0 32 34.5 33 43.0 34 50.0 35 59.0 36 92.0 37 168.5 38 235.0 39 237.0 40 218.0 41 232.5 42 241.5 43 217.5 44 211.0 45 217.5 46 186.5 47 148.0 48 112.0 49 76.5 50 75.0 51 66.0 52 48.5 53 47.0 54 47.5 55 36.5 56 31.5 57 29.0 58 26.5 59 25.5 60 32.0 61 35.5 62 27.0 63 29.5 64 75.5 65 111.0 66 68.0 67 26.5 68 23.5 69 21.5 70 19.5 71 29.0 72 33.5 73 22.5 74 15.0 75 11.5 76 9.0 77 10.5 78 8.0 79 5.5 80 5.5 81 5.5 82 4.0 83 1.5 84 0.5 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.05 #Duplication Level Percentage of deduplicated Percentage of total 1 82.19129193433263 57.574999999999996 2 11.45610278372591 16.05 3 3.033547466095646 6.375 4 1.1420413990007139 3.2 5 0.4282655246252677 1.5 6 0.32119914346895073 1.35 7 0.24982155603140616 1.225 8 0.17844396859386152 1.0 9 0.21413276231263384 1.35 >10 0.7494646680942184 8.825 >50 0.03568879371877231 1.55 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 62 1.55 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 40 1.0 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 29 0.7250000000000001 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 23 0.575 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 23 0.575 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 21 0.525 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 20 0.5 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 18 0.44999999999999996 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 18 0.44999999999999996 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 16 0.4 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 15 0.375 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 15 0.375 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 14 0.35000000000000003 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 14 0.35000000000000003 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 13 0.325 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 12 0.3 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 11 0.27499999999999997 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 11 0.27499999999999997 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 10 0.25 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 10 0.25 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 10 0.25 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 10 0.25 No Hit TCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCG 9 0.22499999999999998 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 9 0.22499999999999998 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 9 0.22499999999999998 No Hit CCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTT 9 0.22499999999999998 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 9 0.22499999999999998 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 9 0.22499999999999998 No Hit AGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 8 0.2 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 8 0.2 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 8 0.2 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 8 0.2 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 8 0.2 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 7 0.17500000000000002 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 7 0.17500000000000002 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 7 0.17500000000000002 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 7 0.17500000000000002 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 7 0.17500000000000002 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 7 0.17500000000000002 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 7 0.17500000000000002 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 6 0.15 No Hit GCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCA 6 0.15 No Hit TCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGT 6 0.15 No Hit TGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAG 6 0.15 No Hit GTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCG 6 0.15 No Hit ATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATA 6 0.15 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 6 0.15 No Hit TGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGA 6 0.15 No Hit TCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTT 6 0.15 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 5 0.125 No Hit GAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAAC 5 0.125 No Hit CTCTCGCAAAAACAGCTCTCTTCATCATTTCTTCACATGTACCCGCAGTT 5 0.125 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 5 0.125 No Hit CGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAAC 5 0.125 No Hit CTCTAATTCAAAACCGAACATGAAATTTTCATTTCATTCGGCTCCTTTAT 5 0.125 No Hit GCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAGCTGCAA 5 0.125 No Hit CACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCA 5 0.125 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 5 0.125 No Hit CAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTG 5 0.125 No Hit CACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCG 5 0.125 No Hit AGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTAT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.025 0.0 0.0 0.0 0.0 92-93 0.025 0.0 0.0 0.0 0.0 94-95 0.025 0.0 0.0 0.0 0.0 96-97 0.05 0.0 0.0 0.0 0.0 98-99 0.05 0.0 0.0 0.0 0.0 100-101 0.05 0.0 0.0 0.0 0.0 102-103 0.05 0.0 0.0 0.0 0.0 104-105 0.05 0.0 0.0 0.0 0.0 106-107 0.05 0.0 0.0 0.0 0.0 108-109 0.0625 0.0 0.0 0.0 0.0 110-111 0.125 0.0 0.0 0.0 0.0 112-113 0.15 0.0 0.0 0.0 0.0 114-115 0.25 0.0 0.0 0.0 0.0 116-117 0.3125 0.0 0.0 0.0 0.0 118-119 0.375 0.0 0.0 0.0 0.0 120-121 0.3875 0.0 0.0 0.0 0.0 122-123 0.4 0.0 0.0 0.0 0.0 124-125 0.4 0.0 0.0 0.0 0.0 126-127 0.4375 0.0 0.0 0.0 0.0 128-129 0.5125 0.0 0.0 0.0 0.0 130-131 0.5375000000000001 0.0 0.0 0.0 0.0 132-133 0.5625 0.0 0.0 0.0 0.0 134-135 0.575 0.0 0.0 0.0 0.0 136-137 0.5874999999999999 0.0 0.0 0.0 0.0 138 0.625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAGTT 10 0.006973645 144.0 3 TCTCGGC 10 0.006973645 144.0 9 TCGTAGT 10 0.006973645 144.0 2 CATCAAT 30 0.0015031899 23.999998 10-14 >>END_MODULE ERR11006586 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006586_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.63425 37.0 37.0 37.0 37.0 37.0 2 36.2395 37.0 37.0 37.0 37.0 37.0 3 36.2625 37.0 37.0 37.0 37.0 37.0 4 36.259 37.0 37.0 37.0 37.0 37.0 5 36.1675 37.0 37.0 37.0 37.0 37.0 6 36.37 37.0 37.0 37.0 37.0 37.0 7 36.343 37.0 37.0 37.0 37.0 37.0 8 36.321 37.0 37.0 37.0 37.0 37.0 9 36.284 37.0 37.0 37.0 37.0 37.0 10-14 36.2992 37.0 37.0 37.0 37.0 37.0 15-19 36.320899999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.2278 37.0 37.0 37.0 37.0 37.0 25-29 36.1918 37.0 37.0 37.0 37.0 37.0 30-34 36.21560000000001 37.0 37.0 37.0 37.0 37.0 35-39 36.1392 37.0 37.0 37.0 37.0 37.0 40-44 36.146 37.0 37.0 37.0 37.0 37.0 45-49 36.145799999999994 37.0 37.0 37.0 37.0 37.0 50-54 36.06850000000001 37.0 37.0 37.0 37.0 37.0 55-59 36.04990000000001 37.0 37.0 37.0 37.0 37.0 60-64 35.93580000000001 37.0 37.0 37.0 37.0 37.0 65-69 35.9757 37.0 37.0 37.0 37.0 37.0 70-74 35.9069 37.0 37.0 37.0 37.0 37.0 75-79 35.902899999999995 37.0 37.0 37.0 37.0 37.0 80-84 35.860699999999994 37.0 37.0 37.0 37.0 37.0 85-89 35.8046 37.0 37.0 37.0 37.0 37.0 90-94 35.7905 37.0 37.0 37.0 37.0 37.0 95-99 35.674800000000005 37.0 37.0 37.0 37.0 37.0 100-104 35.643299999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.5422 37.0 37.0 37.0 37.0 37.0 110-114 35.4958 37.0 37.0 37.0 37.0 37.0 115-119 35.3823 37.0 37.0 37.0 34.6 37.0 120-124 35.374900000000004 37.0 37.0 37.0 32.2 37.0 125-129 35.4179 37.0 37.0 37.0 34.6 37.0 130-134 35.309400000000004 37.0 37.0 37.0 32.2 37.0 135-139 35.218500000000006 37.0 37.0 37.0 25.0 37.0 140-144 35.09 37.0 37.0 37.0 25.0 37.0 145-149 35.201699999999995 37.0 37.0 37.0 27.4 37.0 150 34.7615 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 1.0 21 0.0 22 0.0 23 3.0 24 8.0 25 5.0 26 13.0 27 20.0 28 21.0 29 39.0 30 53.0 31 46.0 32 70.0 33 109.0 34 178.0 35 551.0 36 2717.0 37 165.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.891719745222932 26.063694267515924 16.94267515923567 28.101910828025478 2 26.700000000000003 25.8 29.575000000000003 17.925 3 20.925 29.775000000000002 29.925 19.375 4 24.825 28.299999999999997 26.724999999999998 20.150000000000002 5 24.45 30.15 26.575 18.825 6 21.65 32.300000000000004 27.1 18.95 7 20.625 22.45 39.050000000000004 17.875 8 23.325000000000003 22.975 29.725 23.974999999999998 9 23.200000000000003 20.200000000000003 33.575 23.025000000000002 10-14 25.085 26.075 27.389999999999997 21.45 15-19 25.174999999999997 25.095 28.865000000000002 20.865000000000002 20-24 24.985 24.555 29.165000000000003 21.295 25-29 24.545 24.8 29.494999999999997 21.16 30-34 25.2 23.925 29.520000000000003 21.355 35-39 25.2 24.279999999999998 28.715000000000003 21.805 40-44 24.26 24.815 29.86 21.065 45-49 23.775 25.974999999999998 28.53 21.72 50-54 24.455 25.2 28.499999999999996 21.845 55-59 24.385 24.975 28.27 22.37 60-64 24.15 24.975 28.744999999999997 22.13 65-69 24.310000000000002 25.64 28.24 21.81 70-74 25.1 24.72 28.925 21.255 75-79 24.585 25.235000000000003 29.065 21.115000000000002 80-84 24.485 24.515 29.494999999999997 21.505 85-89 24.45 25.695 27.575 22.28 90-94 23.880000000000003 25.355 28.27 22.495 95-99 23.575 25.259999999999998 29.189999999999998 21.975 100-104 24.69 24.47 29.709999999999997 21.13 105-109 25.4 24.695 28.444999999999997 21.46 110-114 24.12 25.47 29.195 21.215 115-119 24.525 24.8 29.15 21.525 120-124 24.115000000000002 26.035000000000004 28.244999999999997 21.605 125-129 23.665 25.765 28.425 22.145 130-134 23.22 25.669999999999998 28.599999999999998 22.509999999999998 135-139 24.3 25.56 28.935 21.205 140-144 23.875 25.15 29.235 21.740000000000002 145-149 22.755 25.005 29.53 22.71 150 22.725 23.25 29.075 24.95 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.0 16 1.0 17 0.5 18 1.5 19 2.0 20 2.5 21 4.5 22 3.0 23 1.0 24 7.0 25 13.5 26 18.5 27 19.0 28 23.5 29 31.0 30 32.0 31 39.0 32 44.5 33 49.5 34 61.0 35 78.0 36 120.0 37 171.5 38 202.5 39 201.0 40 217.5 41 219.5 42 183.5 43 189.5 44 201.5 45 201.0 46 195.5 47 147.5 48 105.0 49 89.0 50 70.5 51 60.5 52 47.0 53 39.0 54 35.5 55 39.5 56 36.5 57 23.5 58 26.5 59 34.5 60 38.5 61 34.0 62 35.0 63 68.0 64 91.5 65 88.0 66 56.5 67 32.0 68 41.5 69 34.0 70 26.5 71 26.5 72 25.5 73 22.5 74 16.5 75 16.5 76 14.5 77 10.0 78 8.0 79 6.5 80 4.5 81 4.5 82 3.5 83 1.5 84 2.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.875 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.52499999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 82.18293097585855 60.425 2 11.186671200272016 16.45 3 3.2301938116286975 7.124999999999999 4 1.190071404284257 3.5000000000000004 5 0.8160489629377763 3.0 6 0.40802448146888814 1.7999999999999998 7 0.27201632097925876 1.4000000000000001 8 0.13600816048962938 0.8 9 0.10200612036722204 0.675 >10 0.47602856171370284 4.825 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 31 0.775 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 19 0.475 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 15 0.375 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 15 0.375 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 14 0.35000000000000003 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 13 0.325 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 13 0.325 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 11 0.27499999999999997 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 11 0.27499999999999997 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 11 0.27499999999999997 No Hit GTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTC 10 0.25 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 10 0.25 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 10 0.25 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 10 0.25 No Hit CCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGG 9 0.22499999999999998 No Hit CGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGGCC 9 0.22499999999999998 No Hit AGCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTG 9 0.22499999999999998 No Hit GAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCG 8 0.2 No Hit GCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGC 8 0.2 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 8 0.2 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 8 0.2 No Hit TGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCT 7 0.17500000000000002 No Hit GGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGGCCTGGTCTG 7 0.17500000000000002 No Hit GGACGTTGCCGTAGCGCTGCGGGCCTGGTCTGGGTGTGCTACTGATGGCC 7 0.17500000000000002 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 7 0.17500000000000002 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 7 0.17500000000000002 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 7 0.17500000000000002 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 7 0.17500000000000002 No Hit CTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTC 7 0.17500000000000002 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 6 0.15 No Hit GCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTTATG 6 0.15 No Hit GGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATG 6 0.15 No Hit GCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGA 6 0.15 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 6 0.15 No Hit AGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAG 6 0.15 No Hit AACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGG 6 0.15 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 6 0.15 No Hit GAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTA 6 0.15 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 6 0.15 No Hit CTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGGCCTGGTCTGGGT 6 0.15 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 6 0.15 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 5 0.125 No Hit CTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCG 5 0.125 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 5 0.125 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 5 0.125 No Hit GTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTG 5 0.125 No Hit ATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCAC 5 0.125 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 5 0.125 No Hit CGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCC 5 0.125 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 5 0.125 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 5 0.125 No Hit GTAGCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGT 5 0.125 No Hit AGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGT 5 0.125 No Hit GGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTT 5 0.125 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 5 0.125 No Hit GGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGGCCTGGTC 5 0.125 No Hit GTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACT 5 0.125 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 5 0.125 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 5 0.125 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 5 0.125 No Hit TAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAG 5 0.125 No Hit TTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGA 5 0.125 No Hit CACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTAT 5 0.125 No Hit AACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGG 5 0.125 No Hit GAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.025 0.0 0.0 0.0 0.0 92-93 0.025 0.0 0.0 0.0 0.0 94-95 0.025 0.0 0.0 0.0 0.0 96-97 0.05 0.0 0.0 0.0 0.0 98-99 0.05 0.0 0.0 0.0 0.0 100-101 0.05 0.0 0.0 0.0 0.0 102-103 0.05 0.0 0.0 0.0 0.0 104-105 0.05 0.0 0.0 0.0 0.0 106-107 0.05 0.0 0.0 0.0 0.0 108-109 0.0625 0.0 0.0 0.0 0.0 110-111 0.125 0.0 0.0 0.0 0.0 112-113 0.15 0.0 0.0 0.0 0.0 114-115 0.25 0.0 0.0 0.0 0.0 116-117 0.3125 0.0 0.0 0.0 0.0 118-119 0.375 0.0 0.0 0.0 0.0 120-121 0.3875 0.0 0.0 0.0 0.0 122-123 0.4 0.0 0.0 0.0 0.0 124-125 0.4 0.0 0.0 0.0 0.0 126-127 0.4375 0.0 0.0 0.0 0.0 128-129 0.5125 0.0 0.0 0.0 0.0 130-131 0.5375000000000001 0.0 0.0 0.0 0.0 132-133 0.55 0.0 0.0 0.0 0.0 134-135 0.55 0.0 0.0 0.0 0.0 136-137 0.5625 0.0 0.0 0.0 0.0 138 0.6 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 2815209 spots for ERR11006586.sra Written 2815209 spots for ERR11006586.sra Read 2815209 spots for ERR11006586.sra Written 2815209 spots for ERR11006586.sra Read 2815209 spots for ERR11006586.sra Written 2815209 spots for ERR11006586.sra Read 2815209 spots for ERR11006586.sra Written 2815209 spots for ERR11006586.sra Read 2815209 spots for ERR11006586.sra Written 2815209 spots for ERR11006586.sra Read 2815209 spots for ERR11006586.sra Written 2815209 spots for ERR11006586.sra Read 2815209 spots for ERR11006586.sra Written 2815209 spots for ERR11006586.sra Read 2815209 spots for ERR11006586.sra Written 2815209 spots for ERR11006586.sra Read 2815209 spots for ERR11006586.sra Written 2815209 spots for ERR11006586.sra Read 2815209 spots for ERR11006586.sra Written 2815209 spots for ERR11006586.sra Read 2815209 spots for ERR11006586.sra Written 2815209 spots for ERR11006586.sra Read 2815209 spots for ERR11006586.sra Written 2815209 spots for ERR11006586.sra Read 2815209 spots for ERR11006586.sra Written 2815209 spots for ERR11006586.sra Read 2815209 spots for ERR11006586.sra Written 2815209 spots for ERR11006586.sra Read 2815209 spots for ERR11006586.sra Written 2815209 spots for ERR11006586.sra Read 2815209 spots for ERR11006586.sra Written 2815209 spots for ERR11006586.sra Read 2815209 spots for ERR11006586.sra Written 2815209 spots for ERR11006586.sra Read 2815209 spots for ERR11006586.sra Written 2815209 spots for ERR11006586.sra Read 2815209 spots for ERR11006586.sra Written 2815209 spots for ERR11006586.sra Read 2815222 spots for ERR11006586.sra Written 2815222 spots for ERR11006586.sra SRR ids: ['ERR11006586.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_eav5bkq4 ERR11006586.sra spots: 56304193 blocks: [[1, 2815209], [2815210, 5630418], [5630419, 8445627], [8445628, 11260836], [11260837, 14076045], [14076046, 16891254], [16891255, 19706463], [19706464, 22521672], [22521673, 25336881], [25336882, 28152090], [28152091, 30967299], [30967300, 33782508], [33782509, 36597717], [36597718, 39412926], [39412927, 42228135], [42228136, 45043344], [45043345, 47858553], [47858554, 50673762], [50673763, 53488971], [53488972, 56304193]] ERR11006586 file size 20688971 ERR11006586 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006586 ERR11006586_1.fastq ERR11006586_2.fastq Input file: ERR11006586_1.fastq Paired file: ERR11006586_2.fastq trimmed: ERR11006586-trimmed-pair1.fastq, ERR11006586-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 18:21:41 2024 >> started Fri Dec 6 18:24:41 2024 >> done (179.806s) 56304193 read pairs processed; of these: 61 ( 0.00%) short read pairs filtered out after trimming by size control 654 ( 0.00%) empty read pairs filtered out after trimming by size control 56303478 (100.00%) read pairs available; of these: 498498 ( 0.89%) trimmed read pairs available after processing 55804980 (99.11%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 3 0.00% 20 0 0.00% 21 2 0.00% 22 13 0.00% 23 6 0.00% 24 10 0.00% 25 22 0.00% 26 8 0.00% 27 25 0.00% 28 24 0.00% 29 22 0.00% 30 12 0.00% 31 62 0.00% 32 12 0.00% 33 8 0.00% 34 21 0.00% 35 20 0.00% 36 17 0.00% 37 12 0.00% 38 19 0.00% 39 13 0.00% 40 27 0.00% 41 23 0.00% 42 26 0.00% 43 23 0.00% 44 29 0.00% 45 33 0.00% 46 28 0.00% 47 37 0.00% 48 38 0.00% 49 33 0.00% 50 38 0.00% 51 44 0.00% 52 56 0.00% 53 73 0.00% 54 46 0.00% 55 52 0.00% 56 80 0.00% 57 82 0.00% 58 87 0.00% 59 83 0.00% 60 92 0.00% 61 109 0.00% 62 137 0.00% 63 144 0.00% 64 138 0.00% 65 170 0.00% 66 207 0.00% 67 188 0.00% 68 205 0.00% 69 224 0.00% 70 268 0.00% 71 256 0.00% 72 311 0.00% 73 313 0.00% 74 372 0.00% 75 429 0.00% 76 402 0.00% 77 527 0.00% 78 459 0.00% 79 491 0.00% 80 511 0.00% 81 562 0.00% 82 647 0.00% 83 687 0.00% 84 776 0.00% 85 904 0.00% 86 884 0.00% 87 1073 0.00% 88 1111 0.00% 89 1105 0.00% 90 1231 0.00% 91 1327 0.00% 92 1393 0.00% 93 1405 0.00% 94 1471 0.00% 95 1574 0.00% 96 1768 0.00% 97 1914 0.00% 98 2038 0.00% 99 2157 0.00% 100 2251 0.00% 101 2314 0.00% 102 2384 0.00% 103 2535 0.00% 104 2655 0.00% 105 2799 0.00% 106 3006 0.01% 107 3356 0.01% 108 3326 0.01% 109 3603 0.01% 110 3923 0.01% 111 4011 0.01% 112 4272 0.01% 113 4236 0.01% 114 4468 0.01% 115 4812 0.01% 116 5262 0.01% 117 5419 0.01% 118 5625 0.01% 119 6009 0.01% 120 6427 0.01% 121 7010 0.01% 122 7173 0.01% 123 7662 0.01% 124 7953 0.01% 125 8332 0.01% 126 8212 0.01% 127 8394 0.01% 128 8869 0.02% 129 9511 0.02% 130 10332 0.02% 131 10868 0.02% 132 11193 0.02% 133 11114 0.02% 134 11457 0.02% 135 11771 0.02% 136 12299 0.02% 137 12991 0.02% 138 13491 0.02% 139 14550 0.03% 140 15081 0.03% 141 16084 0.03% 142 16993 0.03% 143 17810 0.03% 144 18142 0.03% 145 19314 0.03% 146 21021 0.04% 147 21094 0.04% 148 22368 0.04% 149 23463 0.04% 150 55804980 99.11% 56303478 reads passed initial QC criterion=sequence-density sequence-density=0.57 sequence-density-rank=1 fanout-score=2.54 fanout-score-rank=26 prefix-density=0.62 prefix-fanout=2.3 sequence=TCCTCTTCCCCA criterion=fanout-score sequence-density=0.03 sequence-density-rank=32 fanout-score=46.36 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=5.5 sequence=ATAAAAAAAAGGGGGGGTAAGGACCCGCTAAGCTCCTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGATGAACCCAATCCAGAATATGAACCATAAAAGAAAACACCTACTAAACCAATCACAAGAATACCAGTTACCGTACCTATCAGCCAAAGAGGAATTCTTCCAGTAGTATCGGCCATTTCCCCTACTTTCCTCCACATTTTATCAAGTGGTCATGCTAGAGACAAAAACAGTCATGGATAGTTATGTTATAAGGATGGTATCCTTCCAAATGGGATAAGAGAGTTCTTACTACTCTCTTCTTTTCTCTCAATTAAAGAAGTAATTGGAAAACAAAACAGCAAGTACAAAAATGAGTAATAAACCCCAGTATAGACTGGTACGATTCAATTCAACATTTTGTTCATTCGGGTTTGATTGTGTCATAGTTCTATAGTTGGAATTTAGTTTATCGTTGGATGAACTGCATTGCTGATATTGATCCCAAGAAAAAAA criterion=sequence-density sequence-density=0.75 sequence-density-rank=1 fanout-score=4.54 fanout-score-rank=20 prefix-density=1.12 prefix-fanout=3.0 sequence=TGGGGAAGAGGA criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=521.37 fanout-score-rank=1 prefix-density=3.08 prefix-fanout=1.0 sequence=ACTTCTTACTTCCATTAGTCCCCGTGTTCTTCGAATGGATCTCTTAATTGTTGAGAGGGTTGCCCAAACGCGGTATATAAGGCATACCCAGTAAAGCTTACAAGTAAACCAGATATGGAGATGGCGACTAAAGTTGCTGTTTCCATTTTTATAGAATTTAAAGATTACAATGGATCTACAAAAAGATCGT ERR11006586 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 18:25:26 Started mapping on | Dec 06 18:25:26 Finished on | Dec 06 18:31:33 Mapping speed, Million of reads per hour | 552.30 Number of input reads | 56303478 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 39612601 Uniquely mapped reads % | 70.36% Average mapped length | 298.34 Number of splices: Total | 14998179 Number of splices: Annotated (sjdb) | 14055604 Number of splices: GT/AG | 14624482 Number of splices: GC/AG | 192197 Number of splices: AT/AC | 31745 Number of splices: Non-canonical | 149755 Mismatch rate per base, % | 0.31% Deletion rate per base | 0.02% Deletion average length | 1.67 Insertion rate per base | 0.01% Insertion average length | 1.98 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 14879820 % of reads mapped to multiple loci | 26.43% Number of reads mapped to too many loci | 21760 % of reads mapped to too many loci | 0.04% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.68% % of reads unmapped: other | 0.50% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1811057 1811057 1811057 N_multimapping 14879820 14879820 14879820 N_noFeature 9424366 36079059 11899444 N_ambiguous 1999114 55283 977829 UnstrandedReadsAssigned:28189121 PositiveStrandReadsAssigned:3478259 NegativeStrandReadsAssigned:26735328 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006586 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006586-trimmed-pair1.fastq ERR11006586-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 56,303,478 reads, 32,026,484 reads pseudoaligned [quant] estimated average fragment length: 265.757 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,103 rounds 52973 ERR11006586.ke.tsv 35125 ERR11006586.se.tsv 88098 total ==> ERR11006586.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 671.487 0 0 PNS24247 1044 779.243 14.1227 0.561991 PNS24249 1928 1663.24 162.385 3.02743 PNS24246 1044 779.243 14.1227 0.561991 PNS24248 1044 779.243 14.1227 0.561991 PNS24244 1471 1206.24 31.247 0.803264 PNS24243 293 53.7107 1 0.577329 KQK14069 1603 1338.24 1738.07 40.2734 KQK14071 474 211.208 83.2007 12.2152 ==> ERR11006586.se.tsv <== BRADI_1g14170v3 1962 BRADI_1g53295v3 113 BRADI_1g59795v3 241 BRADI_1g07683v3 1 BRADI_1g00485v3 7 BRADI_1g20270v3 127 BRADI_1g74790v3 315 BRADI_1g09890v3 0 BRADI_1g77505v3 193 BRADI_1g48960v3 5 ERR11006586 completed mapping pipeline successfully