Starting /dee2/code/volunteer_pipeline.sh ERR11006587 current disk space = 1550503657472 free memory = 1601798736 ERR11006587 SRAfilesize 8ea6400cd2ab02e337c8ce4adbf6d7d8 ERR11006587.sra ERR11006587.sra file validated ERR11006587 is paired end ERR11006587 is conventional basespace ERR11006587 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006587_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.32 37.0 37.0 37.0 37.0 37.0 2 36.37 37.0 37.0 37.0 37.0 37.0 3 36.3815 37.0 37.0 37.0 37.0 37.0 4 36.3815 37.0 37.0 37.0 37.0 37.0 5 36.521 37.0 37.0 37.0 37.0 37.0 6 36.5425 37.0 37.0 37.0 37.0 37.0 7 36.3145 37.0 37.0 37.0 37.0 37.0 8 36.33975 37.0 37.0 37.0 37.0 37.0 9 36.461 37.0 37.0 37.0 37.0 37.0 10-14 36.4539 37.0 37.0 37.0 37.0 37.0 15-19 36.439 37.0 37.0 37.0 37.0 37.0 20-24 36.377599999999994 37.0 37.0 37.0 37.0 37.0 25-29 36.322199999999995 37.0 37.0 37.0 37.0 37.0 30-34 36.3217 37.0 37.0 37.0 37.0 37.0 35-39 36.259100000000004 37.0 37.0 37.0 37.0 37.0 40-44 36.1872 37.0 37.0 37.0 37.0 37.0 45-49 36.1909 37.0 37.0 37.0 37.0 37.0 50-54 36.1273 37.0 37.0 37.0 37.0 37.0 55-59 36.1604 37.0 37.0 37.0 37.0 37.0 60-64 36.0904 37.0 37.0 37.0 37.0 37.0 65-69 36.056999999999995 37.0 37.0 37.0 37.0 37.0 70-74 36.0633 37.0 37.0 37.0 37.0 37.0 75-79 35.971599999999995 37.0 37.0 37.0 37.0 37.0 80-84 35.9777 37.0 37.0 37.0 37.0 37.0 85-89 35.963800000000006 37.0 37.0 37.0 37.0 37.0 90-94 35.873599999999996 37.0 37.0 37.0 37.0 37.0 95-99 35.875699999999995 37.0 37.0 37.0 37.0 37.0 100-104 35.8574 37.0 37.0 37.0 37.0 37.0 105-109 35.7471 37.0 37.0 37.0 37.0 37.0 110-114 35.737700000000004 37.0 37.0 37.0 37.0 37.0 115-119 35.8186 37.0 37.0 37.0 37.0 37.0 120-124 35.5198 37.0 37.0 37.0 37.0 37.0 125-129 35.4544 37.0 37.0 37.0 37.0 37.0 130-134 35.4744 37.0 37.0 37.0 37.0 37.0 135-139 35.4288 37.0 37.0 37.0 37.0 37.0 140-144 35.287800000000004 37.0 37.0 37.0 32.2 37.0 145-149 35.2166 37.0 37.0 37.0 29.8 37.0 150 35.329 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 1.0 20 0.0 21 1.0 22 0.0 23 1.0 24 3.0 25 4.0 26 5.0 27 14.0 28 18.0 29 32.0 30 51.0 31 70.0 32 79.0 33 121.0 34 165.0 35 368.0 36 2816.0 37 250.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.15 13.825000000000001 14.725 34.300000000000004 2 28.299999999999997 12.475 27.3 31.924999999999997 3 23.625 17.599999999999998 24.4 34.375 4 27.175 19.5 22.35 30.975 5 28.425 23.7 22.575 25.3 6 26.200000000000003 30.15 21.05 22.6 7 16.725 31.674999999999997 33.45 18.15 8 17.221526908635795 30.112640801001252 30.738423028785984 21.927409261576972 9 17.424999999999997 27.575 33.425 21.575 10-14 20.36 32.09 25.924999999999997 21.625 15-19 20.86 30.904999999999998 25.6 22.634999999999998 20-24 20.21 29.685 25.97 24.135 25-29 21.73 30.075000000000003 26.119999999999997 22.075 30-34 22.095000000000002 30.214999999999996 26.0 21.69 35-39 21.525 30.680000000000003 25.895000000000003 21.9 40-44 21.36 29.79 25.990000000000002 22.86 45-49 20.895 29.53 26.810000000000002 22.765 50-54 22.075 30.64 25.85 21.435000000000002 55-59 21.745 28.955 25.374999999999996 23.925 60-64 20.745 30.385 26.340000000000003 22.53 65-69 21.38 30.990000000000002 25.564999999999998 22.065 70-74 21.654999999999998 30.575000000000003 24.745 23.025000000000002 75-79 21.325 29.9 25.94 22.835 80-84 21.560000000000002 30.009999999999998 25.0 23.43 85-89 21.545 29.225 26.51 22.720000000000002 90-94 20.145 30.37 25.985000000000003 23.5 95-99 21.044999999999998 29.835 25.814999999999998 23.305 100-104 20.39 31.240000000000002 24.725 23.645 105-109 21.61 29.189999999999998 25.935000000000002 23.265 110-114 21.240000000000002 29.310000000000002 25.865 23.585 115-119 21.195 29.665000000000003 26.115 23.025000000000002 120-124 20.51 29.615000000000002 25.66 24.215 125-129 21.044999999999998 29.755 24.86 24.34 130-134 21.6 30.195 25.025 23.18 135-139 21.84 29.645 25.36 23.155 140-144 22.435 29.735 25.945 21.884999999999998 145-149 21.78 30.025000000000002 25.095 23.1 150 22.275 27.775 25.3 24.65 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.0 5 1.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.0 18 0.5 19 0.5 20 1.0 21 2.0 22 2.0 23 5.0 24 8.0 25 12.0 26 17.5 27 15.0 28 16.5 29 21.5 30 29.0 31 46.0 32 52.5 33 57.0 34 67.5 35 86.5 36 114.5 37 172.0 38 220.0 39 204.0 40 215.5 41 255.5 42 237.0 43 218.5 44 225.0 45 210.0 46 174.5 47 140.5 48 131.0 49 117.5 50 91.0 51 71.0 52 58.0 53 51.5 54 47.5 55 40.0 56 37.5 57 35.0 58 35.0 59 35.0 60 30.0 61 27.0 62 25.5 63 24.0 64 30.0 65 43.5 66 33.5 67 24.5 68 29.0 69 21.0 70 18.0 71 16.5 72 14.5 73 13.0 74 10.0 75 13.5 76 14.5 77 8.5 78 6.5 79 6.0 80 2.5 81 2.0 82 2.0 83 2.0 84 1.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.125 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.5 #Duplication Level Percentage of deduplicated Percentage of total 1 84.93548387096774 65.825 2 10.064516129032258 15.6 3 2.3870967741935485 5.55 4 1.1935483870967742 3.6999999999999997 5 0.3548387096774194 1.375 6 0.3225806451612903 1.5 7 0.16129032258064516 0.8750000000000001 8 0.0967741935483871 0.6 9 0.03225806451612903 0.22499999999999998 >10 0.4516129032258065 4.75 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 25 0.625 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 16 0.4 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 15 0.375 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 14 0.35000000000000003 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 14 0.35000000000000003 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 14 0.35000000000000003 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 13 0.325 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 13 0.325 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 12 0.3 No Hit CAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAA 11 0.27499999999999997 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 11 0.27499999999999997 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 11 0.27499999999999997 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 11 0.27499999999999997 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 10 0.25 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 9 0.22499999999999998 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 8 0.2 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 8 0.2 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 8 0.2 No Hit GCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCA 7 0.17500000000000002 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 7 0.17500000000000002 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 7 0.17500000000000002 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 7 0.17500000000000002 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 7 0.17500000000000002 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 6 0.15 No Hit GCAGTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCG 6 0.15 No Hit GGGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGC 6 0.15 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 6 0.15 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 6 0.15 No Hit ACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACT 6 0.15 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 6 0.15 No Hit CCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTT 6 0.15 No Hit TCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGT 6 0.15 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 6 0.15 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 5 0.125 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 5 0.125 No Hit GTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCA 5 0.125 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 5 0.125 No Hit GAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAAC 5 0.125 No Hit CCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAG 5 0.125 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 5 0.125 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 5 0.125 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 5 0.125 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 5 0.125 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.037500000000000006 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.0625 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.075 0.0 0.0 0.0 0.0 86-87 0.1 0.0 0.0 0.0 0.0 88-89 0.15 0.0 0.0 0.0 0.0 90-91 0.15 0.0 0.0 0.0 0.0 92-93 0.2 0.0 0.0 0.0 0.0 94-95 0.275 0.0 0.0 0.0 0.0 96-97 0.3 0.0 0.0 0.0 0.0 98-99 0.3875 0.0 0.0 0.0 0.0 100-101 0.4375 0.0 0.0 0.0 0.0 102-103 0.5249999999999999 0.0 0.0 0.0 0.0 104-105 0.6625000000000001 0.0 0.0 0.0 0.0 106-107 0.775 0.0 0.0 0.0 0.0 108-109 0.825 0.0 0.0 0.0 0.0 110-111 0.9375 0.0 0.0 0.0 0.0 112-113 1.0750000000000002 0.0 0.0 0.0 0.0 114-115 1.1875 0.0 0.0 0.0 0.0 116-117 1.45 0.0 0.0 0.0 0.0 118-119 1.6124999999999998 0.0 0.0 0.0 0.0 120-121 1.775 0.0 0.0 0.0 0.0 122-123 1.9375 0.0 0.0 0.0 0.0 124-125 2.2249999999999996 0.0 0.0 0.0 0.0 126-127 2.4749999999999996 0.0 0.0 0.0 0.0 128-129 2.7375 0.0 0.0 0.0 0.0 130-131 2.9875 0.0 0.0 0.0 0.0 132-133 3.3 0.0 0.0 0.0 0.0 134-135 3.675 0.0 0.0 0.0 0.0 136-137 4.1375 0.0 0.0 0.0 0.0 138 4.475 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAACCA 10 0.006973645 144.0 5 TCTTTTC 10 0.006973645 144.0 5 CTCAACC 10 0.006973645 144.0 4 TGATGAT 35 0.0034045284 61.714283 8 GTTGATG 35 0.0034045284 61.714283 6 GGTTGAT 35 0.0034045284 61.714283 5 GATGATA 35 0.0034045284 61.714283 9 GCACGGT 35 0.0034045284 61.714283 1 CGGTTGA 35 0.0034045284 61.714283 4 ACGGTTG 35 0.0034045284 61.714283 3 CACGGTT 35 0.0034045284 61.714283 2 TTGATGA 40 0.005777437 54.0 7 >>END_MODULE ERR11006587 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006587_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0105 37.0 37.0 37.0 37.0 37.0 2 36.1895 37.0 37.0 37.0 37.0 37.0 3 36.1235 37.0 37.0 37.0 37.0 37.0 4 36.235 37.0 37.0 37.0 37.0 37.0 5 36.2435 37.0 37.0 37.0 37.0 37.0 6 36.268 37.0 37.0 37.0 37.0 37.0 7 36.224 37.0 37.0 37.0 37.0 37.0 8 36.188 37.0 37.0 37.0 37.0 37.0 9 36.1735 37.0 37.0 37.0 37.0 37.0 10-14 36.220299999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.19799999999999 37.0 37.0 37.0 37.0 37.0 20-24 36.2008 37.0 37.0 37.0 37.0 37.0 25-29 36.2017 37.0 37.0 37.0 37.0 37.0 30-34 36.1769 37.0 37.0 37.0 37.0 37.0 35-39 36.123000000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.0644 37.0 37.0 37.0 37.0 37.0 45-49 36.009699999999995 37.0 37.0 37.0 37.0 37.0 50-54 35.9581 37.0 37.0 37.0 37.0 37.0 55-59 35.928200000000004 37.0 37.0 37.0 37.0 37.0 60-64 35.8572 37.0 37.0 37.0 37.0 37.0 65-69 35.8319 37.0 37.0 37.0 37.0 37.0 70-74 35.7495 37.0 37.0 37.0 37.0 37.0 75-79 35.778200000000005 37.0 37.0 37.0 37.0 37.0 80-84 35.8229 37.0 37.0 37.0 37.0 37.0 85-89 35.7342 37.0 37.0 37.0 37.0 37.0 90-94 35.7019 37.0 37.0 37.0 37.0 37.0 95-99 35.571600000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.6307 37.0 37.0 37.0 37.0 37.0 105-109 35.4839 37.0 37.0 37.0 37.0 37.0 110-114 35.4371 37.0 37.0 37.0 37.0 37.0 115-119 35.370900000000006 37.0 37.0 37.0 34.6 37.0 120-124 35.3151 37.0 37.0 37.0 32.2 37.0 125-129 35.2016 37.0 37.0 37.0 25.0 37.0 130-134 35.1368 37.0 37.0 37.0 27.4 37.0 135-139 35.058400000000006 37.0 37.0 37.0 25.0 37.0 140-144 35.0029 37.0 37.0 37.0 25.0 37.0 145-149 34.934999999999995 37.0 37.0 37.0 25.0 37.0 150 34.8415 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 1.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.0 22 2.0 23 5.0 24 4.0 25 17.0 26 9.0 27 10.0 28 31.0 29 20.0 30 44.0 31 61.0 32 90.0 33 129.0 34 221.0 35 648.0 36 2571.0 37 135.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.775000000000002 25.474999999999998 17.724999999999998 28.025 2 24.975 27.6 32.0 15.425 3 21.95 28.325 31.900000000000002 17.825 4 24.375 30.525000000000002 24.825 20.275000000000002 5 23.974999999999998 31.974999999999998 26.375 17.675 6 21.224999999999998 36.425000000000004 25.05 17.299999999999997 7 19.925 21.975 38.3 19.8 8 20.825 24.9 30.8 23.474999999999998 9 23.200000000000003 22.875 32.35 21.575 10-14 23.89 27.725 27.49 20.895 15-19 24.11 26.1 29.115000000000002 20.674999999999997 20-24 23.745 26.424999999999997 28.749999999999996 21.08 25-29 23.705000000000002 26.265 29.385 20.645 30-34 23.54 26.05 30.395 20.015 35-39 23.835 25.575 29.78 20.810000000000002 40-44 23.835 26.355 29.175 20.635 45-49 23.755000000000003 26.36 29.035 20.849999999999998 50-54 24.23 25.83 29.115000000000002 20.825 55-59 23.59 26.179999999999996 29.345 20.885 60-64 22.615 25.885 29.909999999999997 21.59 65-69 23.455000000000002 25.97 29.580000000000002 20.995 70-74 23.775 26.205000000000002 28.935 21.085 75-79 23.56 26.165 29.134999999999998 21.14 80-84 24.0 25.64 29.455 20.905 85-89 23.5 26.224999999999998 29.18 21.095 90-94 22.8 26.195 29.959999999999997 21.044999999999998 95-99 23.294999999999998 25.290000000000003 30.225 21.19 100-104 23.74 24.92 30.345 20.995 105-109 24.485 25.595000000000002 29.28 20.64 110-114 23.615 26.02 29.654999999999998 20.71 115-119 23.799999999999997 25.7 29.575000000000003 20.925 120-124 23.75 26.22 28.73 21.3 125-129 23.72 26.179999999999996 28.970000000000002 21.13 130-134 23.235 26.345000000000002 29.005 21.415 135-139 23.990000000000002 26.255 29.549999999999997 20.205000000000002 140-144 24.055 26.479999999999997 29.14 20.325 145-149 23.84 26.174999999999997 29.42 20.565 150 22.725 25.650000000000002 28.849999999999998 22.775000000000002 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.5 19 1.5 20 2.5 21 2.5 22 3.5 23 5.5 24 6.5 25 8.0 26 10.0 27 20.5 28 34.0 29 36.0 30 42.5 31 54.0 32 56.0 33 56.0 34 70.0 35 92.0 36 132.5 37 199.5 38 221.0 39 201.5 40 204.0 41 222.0 42 208.5 43 213.0 44 233.5 45 217.5 46 188.0 47 138.5 48 104.0 49 87.5 50 88.0 51 76.5 52 53.5 53 42.0 54 35.5 55 35.5 56 28.5 57 31.0 58 33.0 59 33.5 60 37.0 61 40.5 62 33.5 63 31.5 64 42.0 65 33.0 66 25.0 67 27.5 68 28.0 69 24.5 70 21.5 71 24.0 72 23.5 73 18.5 74 12.5 75 9.5 76 8.0 77 6.0 78 6.0 79 4.5 80 1.5 81 1.5 82 3.0 83 2.0 84 0.0 85 1.5 86 1.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.67500000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 84.55101383971677 65.675 2 10.170582555519795 15.8 3 2.5426456388799488 5.925 4 0.9977470228516253 3.1 5 0.7080785323463148 2.75 6 0.32185387833923396 1.5 7 0.22529771483746378 1.225 8 0.0643707756678468 0.4 9 0.16092693916961698 1.125 >10 0.2574831026713872 2.5 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 19 0.475 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 14 0.35000000000000003 No Hit TGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCT 12 0.3 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 12 0.3 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 12 0.3 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 11 0.27499999999999997 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 10 0.25 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 10 0.25 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 9 0.22499999999999998 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 9 0.22499999999999998 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 9 0.22499999999999998 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 9 0.22499999999999998 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 9 0.22499999999999998 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 8 0.2 No Hit AGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGC 8 0.2 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 7 0.17500000000000002 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 7 0.17500000000000002 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 7 0.17500000000000002 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 7 0.17500000000000002 No Hit CTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTT 7 0.17500000000000002 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 7 0.17500000000000002 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 7 0.17500000000000002 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 6 0.15 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 6 0.15 No Hit AAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAG 6 0.15 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 6 0.15 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 6 0.15 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 6 0.15 No Hit TGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAAT 6 0.15 No Hit GTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTG 6 0.15 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 6 0.15 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 6 0.15 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 5 0.125 No Hit GACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCC 5 0.125 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 5 0.125 No Hit TATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACT 5 0.125 No Hit TTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGCT 5 0.125 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 5 0.125 No Hit TTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAAC 5 0.125 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 5 0.125 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 5 0.125 No Hit AAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATC 5 0.125 No Hit TTCACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCT 5 0.125 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 5 0.125 No Hit TGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCAT 5 0.125 No Hit CATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGA 5 0.125 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 5 0.125 No Hit CTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATT 5 0.125 No Hit GTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCG 5 0.125 No Hit TCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACT 5 0.125 No Hit CTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTAC 5 0.125 No Hit AATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTA 5 0.125 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 5 0.125 No Hit GAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.037500000000000006 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.0875 0.0 0.0 0.0 0.0 78-79 0.1 0.0 0.0 0.0 0.0 80-81 0.1 0.0 0.0 0.0 0.0 82-83 0.1 0.0 0.0 0.0 0.0 84-85 0.1 0.0 0.0 0.0 0.0 86-87 0.125 0.0 0.0 0.0 0.0 88-89 0.175 0.0 0.0 0.0 0.0 90-91 0.175 0.0 0.0 0.0 0.0 92-93 0.2125 0.0 0.0 0.0 0.0 94-95 0.275 0.0 0.0 0.0 0.0 96-97 0.3 0.0 0.0 0.0 0.0 98-99 0.4375 0.0 0.0 0.0 0.0 100-101 0.4875 0.0 0.0 0.0 0.0 102-103 0.575 0.0 0.0 0.0 0.0 104-105 0.6875 0.0 0.0 0.0 0.0 106-107 0.775 0.0 0.0 0.0 0.0 108-109 0.85 0.0 0.0 0.0 0.0 110-111 0.9625 0.0 0.0 0.0 0.0 112-113 1.0750000000000002 0.0 0.0 0.0 0.0 114-115 1.1875 0.0 0.0 0.0 0.0 116-117 1.4375 0.0 0.0 0.0 0.0 118-119 1.6375000000000002 0.0 0.0 0.0 0.0 120-121 1.8 0.0 0.0 0.0 0.0 122-123 1.975 0.0 0.0 0.0 0.0 124-125 2.2625 0.0 0.0 0.0 0.0 126-127 2.5250000000000004 0.0 0.0 0.0 0.0 128-129 2.775 0.0 0.0 0.0 0.0 130-131 3.0375 0.0 0.0 0.0 0.0 132-133 3.3499999999999996 0.0 0.0 0.0 0.0 134-135 3.725 0.0 0.0 0.0 0.0 136-137 4.175 0.0 0.0 0.0 0.0 138 4.525 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1680639 spots for ERR11006587.sra Written 1680639 spots for ERR11006587.sra Read 1680639 spots for ERR11006587.sra Written 1680639 spots for ERR11006587.sra Read 1680639 spots for ERR11006587.sra Written 1680639 spots for ERR11006587.sra Read 1680639 spots for ERR11006587.sra Written 1680639 spots for ERR11006587.sra Read 1680639 spots for ERR11006587.sra Written 1680639 spots for ERR11006587.sra Read 1680639 spots for ERR11006587.sra Written 1680639 spots for ERR11006587.sra Read 1680639 spots for ERR11006587.sra Written 1680639 spots for ERR11006587.sra Read 1680639 spots for ERR11006587.sra Written 1680639 spots for ERR11006587.sra Read 1680639 spots for ERR11006587.sra Written 1680639 spots for ERR11006587.sra Read 1680639 spots for ERR11006587.sra Written 1680639 spots for ERR11006587.sra Read 1680639 spots for ERR11006587.sra Written 1680639 spots for ERR11006587.sra Read 1680655 spots for ERR11006587.sra Written 1680655 spots for ERR11006587.sra Read 1680639 spots for ERR11006587.sra Written 1680639 spots for ERR11006587.sra Read 1680639 spots for ERR11006587.sra Written 1680639 spots for ERR11006587.sra Read 1680639 spots for ERR11006587.sra Written 1680639 spots for ERR11006587.sra Read 1680639 spots for ERR11006587.sra Written 1680639 spots for ERR11006587.sra Read 1680639 spots for ERR11006587.sra Written 1680639 spots for ERR11006587.sra Read 1680639 spots for ERR11006587.sra Written 1680639 spots for ERR11006587.sra Read 1680639 spots for ERR11006587.sra Written 1680639 spots for ERR11006587.sra Read 1680639 spots for ERR11006587.sra Written 1680639 spots for ERR11006587.sra SRR ids: ['ERR11006587.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_9f3c7x3o ERR11006587.sra spots: 33612796 blocks: [[1, 1680639], [1680640, 3361278], [3361279, 5041917], [5041918, 6722556], [6722557, 8403195], [8403196, 10083834], [10083835, 11764473], [11764474, 13445112], [13445113, 15125751], [15125752, 16806390], [16806391, 18487029], [18487030, 20167668], [20167669, 21848307], [21848308, 23528946], [23528947, 25209585], [25209586, 26890224], [26890225, 28570863], [28570864, 30251502], [30251503, 31932141], [31932142, 33612796]] ERR11006587 file size 12346661 ERR11006587 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006587 ERR11006587_1.fastq ERR11006587_2.fastq Input file: ERR11006587_1.fastq Paired file: ERR11006587_2.fastq trimmed: ERR11006587-trimmed-pair1.fastq, ERR11006587-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 18:23:29 2024 >> started Fri Dec 6 18:24:05 2024 >> done (36.201s) 33612796 read pairs processed; of these: 184 ( 0.00%) short read pairs filtered out after trimming by size control 1182 ( 0.00%) empty read pairs filtered out after trimming by size control 33611430 (100.00%) read pairs available; of these: 2253839 ( 6.71%) trimmed read pairs available after processing 31357591 (93.29%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 8 0.00% 20 11 0.00% 21 15 0.00% 22 21 0.00% 23 11 0.00% 24 11 0.00% 25 1773 0.01% 26 13 0.00% 27 23 0.00% 28 20 0.00% 29 48 0.00% 30 21 0.00% 31 75 0.00% 32 17 0.00% 33 13 0.00% 34 14 0.00% 35 14 0.00% 36 11 0.00% 37 20 0.00% 38 12 0.00% 39 20 0.00% 40 15 0.00% 41 25 0.00% 42 25 0.00% 43 29 0.00% 44 40 0.00% 45 34 0.00% 46 41 0.00% 47 53 0.00% 48 58 0.00% 49 56 0.00% 50 87 0.00% 51 75 0.00% 52 104 0.00% 53 119 0.00% 54 162 0.00% 55 233 0.00% 56 153 0.00% 57 175 0.00% 58 198 0.00% 59 236 0.00% 60 275 0.00% 61 272 0.00% 62 400 0.00% 63 384 0.00% 64 484 0.00% 65 504 0.00% 66 571 0.00% 67 615 0.00% 68 657 0.00% 69 703 0.00% 70 751 0.00% 71 880 0.00% 72 1033 0.00% 73 1248 0.00% 74 1361 0.00% 75 1545 0.00% 76 1836 0.01% 77 1766 0.01% 78 2100 0.01% 79 2263 0.01% 80 2402 0.01% 81 2648 0.01% 82 2835 0.01% 83 3162 0.01% 84 3579 0.01% 85 4223 0.01% 86 4641 0.01% 87 4917 0.01% 88 5333 0.02% 89 5586 0.02% 90 5909 0.02% 91 6273 0.02% 92 6575 0.02% 93 7017 0.02% 94 7526 0.02% 95 8425 0.03% 96 9067 0.03% 97 9528 0.03% 98 9949 0.03% 99 10853 0.03% 100 11256 0.03% 101 11955 0.04% 102 12331 0.04% 103 13091 0.04% 104 13931 0.04% 105 14993 0.04% 106 16222 0.05% 107 16498 0.05% 108 17578 0.05% 109 18467 0.05% 110 19647 0.06% 111 20180 0.06% 112 21804 0.06% 113 21697 0.06% 114 23119 0.07% 115 24405 0.07% 116 26979 0.08% 117 27474 0.08% 118 28224 0.08% 119 30845 0.09% 120 31895 0.09% 121 33294 0.10% 122 34765 0.10% 123 36303 0.11% 124 38461 0.11% 125 40090 0.12% 126 39680 0.12% 127 40434 0.12% 128 42466 0.13% 129 45474 0.14% 130 48290 0.14% 131 50592 0.15% 132 50695 0.15% 133 50733 0.15% 134 52329 0.16% 135 53393 0.16% 136 55095 0.16% 137 57851 0.17% 138 61014 0.18% 139 64993 0.19% 140 67686 0.20% 141 70397 0.21% 142 74197 0.22% 143 74398 0.22% 144 75840 0.23% 145 79811 0.24% 146 84243 0.25% 147 84725 0.25% 148 89368 0.27% 149 90442 0.27% 150 31357591 93.29% 33611430 reads passed initial QC criterion=sequence-density sequence-density=0.44 sequence-density-rank=1 fanout-score=3.09 fanout-score-rank=27 prefix-density=0.52 prefix-fanout=2.6 sequence=TCCTCTTCCCCA criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=115.08 fanout-score-rank=1 prefix-density=0.47 prefix-fanout=3.3 sequence=AGAAAAAAACAAGTTTGCATCTTCAGGAGAATCTATATTTTCGCGAAATGGATCATAATAAAATGGATTTTAGGTATCTAGGGAAAATTCACTTCGAAGTAACTATTTCCTAGATACCTATGCACGGTACTTCACGGTTGAATGAATCAACCTGAAAAATACCTAAAAAAGGCCTAAAGTTAAGGATTTATCAATGGGTAATGTTGCTCCAATACCTAACCAAAGAGCTACTGCAGTACCGATTAAAAAAACGGTCGTAGCTACCGGGCGACGAAATGGATTTTGGAATTTGTTGACATTCTCTAGAAAAGGTACTGTCAATAAGCCTGTTGGCACAGAAACCATTAAGAGAACGCCCAATAACTTATTGGGTACCGTACGGAGTATTTGAAACACGGGAAAGAAGTACCACTCGGGTAATATTTCCAAAGGAGTTGCAAACGGATCCGCGGGTTCACCAATCATTGATGGCTCGAGAACAGCTAAACCTACATTACATGCAATAGTACCT criterion=sequence-density sequence-density=0.53 sequence-density-rank=1 fanout-score=4.76 fanout-score-rank=18 prefix-density=0.85 prefix-fanout=3.0 sequence=TGGGGAAGAGGA criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=141.58 fanout-score-rank=1 prefix-density=0.22 prefix-fanout=5.9 sequence=AAAAAAAGATAAGAAGCAAAGTTTCCCCCTTGAGTTTGAGTGAAAAAACGAAAATGCGAGAAAAATTGCAATCCCTACCACGTAATAGTGCACCTACACGCCTTCATCGACGTTGTTTTTTGACTGGAAGACCTAGAGCTAACTATCGAGATTTTGGGCTATCCGGGCACGTACTTCGAGAAATGGTTTATGAATGTTTGTT ERR11006587 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 18:25:23 Started mapping on | Dec 06 18:25:23 Finished on | Dec 06 18:29:44 Mapping speed, Million of reads per hour | 463.61 Number of input reads | 33611430 Average input read length | 297 UNIQUE READS: Uniquely mapped reads number | 21837870 Uniquely mapped reads % | 64.97% Average mapped length | 296.25 Number of splices: Total | 9247390 Number of splices: Annotated (sjdb) | 8640012 Number of splices: GT/AG | 9003250 Number of splices: GC/AG | 117808 Number of splices: AT/AC | 35685 Number of splices: Non-canonical | 90647 Mismatch rate per base, % | 0.31% Deletion rate per base | 0.02% Deletion average length | 1.69 Insertion rate per base | 0.02% Insertion average length | 1.86 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 10338204 % of reads mapped to multiple loci | 30.76% Number of reads mapped to too many loci | 10528 % of reads mapped to too many loci | 0.03% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.34% % of reads unmapped: other | 0.90% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1435356 1435356 1435356 N_multimapping 10338204 10338204 10338204 N_noFeature 5938457 20170922 7099258 N_ambiguous 1015100 29719 515738 UnstrandedReadsAssigned:14884313 PositiveStrandReadsAssigned:1637229 NegativeStrandReadsAssigned:14222874 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006587 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006587-trimmed-pair1.fastq ERR11006587-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 33,611,430 reads, 20,746,154 reads pseudoaligned [quant] estimated average fragment length: 214.567 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,217 rounds 52973 ERR11006587.ke.tsv 35125 ERR11006587.se.tsv 88098 total ==> ERR11006587.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 723.004 0 0 PNS24247 1044 830.433 17.8369 1.1261 PNS24249 1928 1714.43 39.0388 1.19383 PNS24246 1044 830.433 17.8369 1.1261 PNS24248 1044 830.433 17.8369 1.1261 PNS24244 1471 1257.43 56.4506 2.35369 PNS24243 293 85.8141 0 0 KQK14069 1603 1389.43 838.7 31.6471 KQK14071 474 261.923 151.814 30.388 ==> ERR11006587.se.tsv <== BRADI_1g14170v3 1131 BRADI_1g53295v3 122 BRADI_1g59795v3 191 BRADI_1g07683v3 0 BRADI_1g00485v3 3 BRADI_1g20270v3 125 BRADI_1g74790v3 117 BRADI_1g09890v3 0 BRADI_1g77505v3 151 BRADI_1g48960v3 0 ERR11006587 completed mapping pipeline successfully