Starting /dee2/code/volunteer_pipeline.sh ERR11006588 current disk space = 1550516948992 free memory = 1342037352 ERR11006588 SRAfilesize 7c22c18510a401fb34aee32830e00b73 ERR11006588.sra ERR11006588.sra file validated ERR11006588 is paired end ERR11006588 is conventional basespace ERR11006588 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006588_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.572 37.0 37.0 37.0 37.0 37.0 2 36.259 37.0 37.0 37.0 37.0 37.0 3 36.431 37.0 37.0 37.0 37.0 37.0 4 36.5435 37.0 37.0 37.0 37.0 37.0 5 36.59 37.0 37.0 37.0 37.0 37.0 6 36.539 37.0 37.0 37.0 37.0 37.0 7 36.4575 37.0 37.0 37.0 37.0 37.0 8 36.515 37.0 37.0 37.0 37.0 37.0 9 36.4915 37.0 37.0 37.0 37.0 37.0 10-14 36.4743 37.0 37.0 37.0 37.0 37.0 15-19 36.5006 37.0 37.0 37.0 37.0 37.0 20-24 36.415000000000006 37.0 37.0 37.0 37.0 37.0 25-29 36.37519999999999 37.0 37.0 37.0 37.0 37.0 30-34 36.341899999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.3144 37.0 37.0 37.0 37.0 37.0 40-44 36.2377 37.0 37.0 37.0 37.0 37.0 45-49 36.175799999999995 37.0 37.0 37.0 37.0 37.0 50-54 36.182100000000005 37.0 37.0 37.0 37.0 37.0 55-59 36.2012 37.0 37.0 37.0 37.0 37.0 60-64 36.16459999999999 37.0 37.0 37.0 37.0 37.0 65-69 36.1468 37.0 37.0 37.0 37.0 37.0 70-74 36.122699999999995 37.0 37.0 37.0 37.0 37.0 75-79 36.0974 37.0 37.0 37.0 37.0 37.0 80-84 35.997699999999995 37.0 37.0 37.0 37.0 37.0 85-89 36.014300000000006 37.0 37.0 37.0 37.0 37.0 90-94 35.976 37.0 37.0 37.0 37.0 37.0 95-99 35.926300000000005 37.0 37.0 37.0 37.0 37.0 100-104 35.9852 37.0 37.0 37.0 37.0 37.0 105-109 35.8295 37.0 37.0 37.0 37.0 37.0 110-114 35.8091 37.0 37.0 37.0 37.0 37.0 115-119 35.8003 37.0 37.0 37.0 37.0 37.0 120-124 35.725 37.0 37.0 37.0 37.0 37.0 125-129 35.5769 37.0 37.0 37.0 37.0 37.0 130-134 35.588499999999996 37.0 37.0 37.0 37.0 37.0 135-139 35.56949999999999 37.0 37.0 37.0 37.0 37.0 140-144 35.5432 37.0 37.0 37.0 37.0 37.0 145-149 35.3585 37.0 37.0 37.0 34.6 37.0 150 35.2655 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 0.0 23 0.0 24 4.0 25 8.0 26 10.0 27 21.0 28 13.0 29 38.0 30 39.0 31 56.0 32 73.0 33 105.0 34 136.0 35 317.0 36 2841.0 37 338.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.175 15.1 14.649999999999999 35.075 2 29.26278836509529 13.214643931795386 28.159478435305918 29.36308926780341 3 23.5 15.6 26.75 34.150000000000006 4 29.299999999999997 17.7 22.75 30.25 5 30.65 21.8 22.85 24.7 6 26.474999999999998 29.799999999999997 21.6 22.125 7 14.374999999999998 30.55 35.825 19.25 8 15.55 30.599999999999998 31.4 22.45 9 16.875 28.249999999999996 33.275 21.6 10-14 20.015 31.465 26.634999999999998 21.884999999999998 15-19 21.325 30.490000000000002 25.96 22.225 20-24 21.029999999999998 29.065 27.015 22.89 25-29 21.69 29.815 25.785000000000004 22.71 30-34 22.27 30.330000000000002 25.415 21.985 35-39 22.31 30.035 24.965 22.689999999999998 40-44 20.59 30.245 26.634999999999998 22.53 45-49 21.275 29.685 26.119999999999997 22.919999999999998 50-54 20.49 31.11 25.785000000000004 22.615 55-59 21.825 29.64 25.0 23.535 60-64 20.7 30.69 25.995 22.615 65-69 21.435000000000002 29.79 25.34 23.435 70-74 21.959999999999997 29.13 24.635 24.275 75-79 22.12 29.59 25.629999999999995 22.66 80-84 22.15 29.520000000000003 24.795 23.535 85-89 22.67 27.589999999999996 26.07 23.669999999999998 90-94 21.075 29.665000000000003 25.915 23.345 95-99 21.955 28.83 25.34 23.875 100-104 21.845 29.744999999999997 25.509999999999998 22.900000000000002 105-109 21.515 29.220000000000002 25.729999999999997 23.535 110-114 22.085 28.425 26.025 23.465 115-119 20.285 30.395 25.585 23.735 120-124 21.07 28.910000000000004 25.115 24.905 125-129 21.255 29.26 25.014999999999997 24.47 130-134 22.09 29.849999999999998 25.025 23.035 135-139 22.71 29.849999999999998 24.125 23.315 140-144 22.97 29.175 25.335 22.52 145-149 22.735 29.585 24.645 23.035 150 24.349999999999998 28.475 25.45 21.725 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.5 16 0.5 17 0.0 18 1.0 19 1.5 20 2.5 21 4.0 22 3.0 23 3.0 24 4.0 25 3.0 26 4.5 27 10.0 28 18.0 29 22.5 30 28.0 31 35.5 32 35.0 33 39.5 34 55.0 35 64.0 36 100.5 37 176.5 38 229.5 39 223.5 40 221.5 41 256.0 42 235.0 43 214.5 44 226.5 45 219.5 46 186.0 47 151.0 48 140.5 49 124.5 50 102.5 51 76.0 52 61.0 53 54.0 54 49.0 55 44.5 56 38.0 57 35.0 58 37.5 59 35.5 60 31.5 61 34.0 62 32.5 63 26.0 64 27.0 65 41.5 66 38.0 67 22.5 68 13.5 69 15.0 70 20.0 71 15.5 72 16.5 73 18.0 74 13.5 75 7.0 76 7.0 77 11.0 78 9.5 79 7.0 80 6.0 81 3.0 82 1.5 83 2.0 84 3.0 85 2.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.3 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.2 #Duplication Level Percentage of deduplicated Percentage of total 1 82.28058510638297 61.875 2 12.632978723404257 19.0 3 2.4268617021276593 5.475 4 0.7646276595744681 2.3 5 0.5651595744680852 2.125 6 0.36569148936170215 1.6500000000000001 7 0.36569148936170215 1.925 8 0.2327127659574468 1.4000000000000001 9 0.06648936170212766 0.44999999999999996 >10 0.2992021276595745 3.8 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 46 1.15 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 24 0.6 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 16 0.4 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 13 0.325 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 12 0.3 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 11 0.27499999999999997 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 10 0.25 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 10 0.25 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 10 0.25 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 9 0.22499999999999998 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 9 0.22499999999999998 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 8 0.2 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 8 0.2 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 8 0.2 No Hit CCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTT 8 0.2 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 8 0.2 No Hit CACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCA 8 0.2 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 8 0.2 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 7 0.17500000000000002 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 7 0.17500000000000002 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 7 0.17500000000000002 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 7 0.17500000000000002 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 7 0.17500000000000002 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 7 0.17500000000000002 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 7 0.17500000000000002 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 7 0.17500000000000002 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 7 0.17500000000000002 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 7 0.17500000000000002 No Hit CAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGA 7 0.17500000000000002 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 6 0.15 No Hit CAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAA 6 0.15 No Hit GCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTG 6 0.15 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 6 0.15 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 6 0.15 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 6 0.15 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 6 0.15 No Hit GCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAGCTGCAA 6 0.15 No Hit CAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAA 6 0.15 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 6 0.15 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 6 0.15 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 5 0.125 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 5 0.125 No Hit CGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 5 0.125 No Hit CCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAA 5 0.125 No Hit CAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGG 5 0.125 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 5 0.125 No Hit AGGGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAAC 5 0.125 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 5 0.125 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 5 0.125 No Hit TCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGT 5 0.125 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 5 0.125 No Hit ACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGA 5 0.125 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 5 0.125 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 5 0.125 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 5 0.125 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 5 0.125 No Hit CCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAGG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0125 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0125 80-81 0.05 0.0 0.0 0.0 0.025 82-83 0.05 0.0 0.0 0.0 0.025 84-85 0.05 0.0 0.0 0.0 0.025 86-87 0.0875 0.0 0.0 0.0 0.025 88-89 0.125 0.0 0.0 0.0 0.025 90-91 0.15 0.0 0.0 0.0 0.025 92-93 0.1875 0.0 0.0 0.0 0.025 94-95 0.225 0.0 0.0 0.0 0.025 96-97 0.225 0.0 0.0 0.0 0.025 98-99 0.35 0.0 0.0 0.0 0.025 100-101 0.4125 0.0 0.0 0.0 0.025 102-103 0.45 0.0 0.0 0.0 0.025 104-105 0.5625 0.0 0.0 0.0 0.025 106-107 0.625 0.0 0.0 0.0 0.025 108-109 0.675 0.0 0.0 0.0 0.025 110-111 0.825 0.0 0.0 0.0 0.025 112-113 0.8875 0.0 0.0 0.0 0.025 114-115 0.9375 0.0 0.0 0.0 0.025 116-117 1.075 0.0 0.0 0.0 0.025 118-119 1.2125 0.0 0.0 0.0 0.025 120-121 1.3250000000000002 0.0 0.0 0.0 0.025 122-123 1.4625 0.0 0.0 0.0 0.025 124-125 1.8125 0.0 0.0 0.0 0.025 126-127 1.9749999999999999 0.0 0.0 0.0 0.025 128-129 2.3625 0.0 0.0 0.0 0.025 130-131 2.5250000000000004 0.0 0.0 0.0 0.025 132-133 2.7125000000000004 0.0 0.0 0.0 0.025 134-135 2.9000000000000004 0.0 0.0 0.0 0.025 136-137 3.375 0.0 0.0 0.0 0.025 138 3.725 0.0 0.0 0.0 0.025 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCTTAG 10 0.006973645 144.0 7 CCTGAAT 10 0.006973645 144.0 1 CTTAGAA 10 0.006973645 144.0 9 ATCCTTA 10 0.006973645 144.0 6 >>END_MODULE ERR11006588 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006588_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.994 37.0 37.0 37.0 37.0 37.0 2 35.757 37.0 37.0 37.0 37.0 37.0 3 36.079 37.0 37.0 37.0 37.0 37.0 4 36.109 37.0 37.0 37.0 37.0 37.0 5 36.063 37.0 37.0 37.0 37.0 37.0 6 36.2225 37.0 37.0 37.0 37.0 37.0 7 35.899 37.0 37.0 37.0 37.0 37.0 8 36.168 37.0 37.0 37.0 37.0 37.0 9 36.12 37.0 37.0 37.0 37.0 37.0 10-14 36.178200000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.2229 37.0 37.0 37.0 37.0 37.0 20-24 36.124 37.0 37.0 37.0 37.0 37.0 25-29 36.0533 37.0 37.0 37.0 37.0 37.0 30-34 36.025999999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.009100000000004 37.0 37.0 37.0 37.0 37.0 40-44 35.9819 37.0 37.0 37.0 37.0 37.0 45-49 36.003 37.0 37.0 37.0 37.0 37.0 50-54 35.891799999999996 37.0 37.0 37.0 37.0 37.0 55-59 35.9696 37.0 37.0 37.0 37.0 37.0 60-64 35.842699999999994 37.0 37.0 37.0 37.0 37.0 65-69 35.734700000000004 37.0 37.0 37.0 37.0 37.0 70-74 35.7188 37.0 37.0 37.0 37.0 37.0 75-79 35.736599999999996 37.0 37.0 37.0 37.0 37.0 80-84 35.5544 37.0 37.0 37.0 37.0 37.0 85-89 35.6059 37.0 37.0 37.0 37.0 37.0 90-94 35.5297 37.0 37.0 37.0 37.0 37.0 95-99 35.5954 37.0 37.0 37.0 37.0 37.0 100-104 35.4334 37.0 37.0 37.0 37.0 37.0 105-109 35.3664 37.0 37.0 37.0 32.2 37.0 110-114 35.3183 37.0 37.0 37.0 34.6 37.0 115-119 35.312200000000004 37.0 37.0 37.0 34.6 37.0 120-124 35.1318 37.0 37.0 37.0 27.4 37.0 125-129 35.0724 37.0 37.0 37.0 25.0 37.0 130-134 34.971000000000004 37.0 37.0 37.0 25.0 37.0 135-139 34.8592 37.0 37.0 37.0 25.0 37.0 140-144 34.8641 37.0 37.0 37.0 25.0 37.0 145-149 34.911500000000004 37.0 37.0 37.0 25.0 37.0 150 34.818 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 0.0 18 0.0 19 0.0 20 2.0 21 0.0 22 6.0 23 3.0 24 8.0 25 12.0 26 15.0 27 16.0 28 20.0 29 40.0 30 56.0 31 59.0 32 95.0 33 129.0 34 224.0 35 706.0 36 2473.0 37 135.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 30.65 24.825 16.675 27.85 2 26.6 27.875 31.525 14.000000000000002 3 21.575 29.65 30.625000000000004 18.15 4 24.925 30.175 24.875 20.025000000000002 5 24.95 32.550000000000004 26.125 16.375 6 20.5 36.8 24.05 18.65 7 20.175 22.225 38.85 18.75 8 23.3 23.525 29.5 23.674999999999997 9 23.474999999999998 20.25 33.575 22.7 10-14 23.925 26.955000000000002 27.935 21.185000000000002 15-19 23.205000000000002 26.185000000000002 29.2 21.41 20-24 23.335 25.66 29.815 21.19 25-29 23.674999999999997 25.06 29.599999999999998 21.665 30-34 24.22 25.515 29.365000000000002 20.9 35-39 24.425 25.465 28.79 21.32 40-44 24.154999999999998 25.814999999999998 28.825 21.205 45-49 24.11 25.480000000000004 29.29 21.12 50-54 23.474999999999998 25.679999999999996 29.475 21.37 55-59 23.765 25.31 28.585 22.34 60-64 23.419999999999998 25.69 29.325000000000003 21.565 65-69 24.08 25.874999999999996 28.560000000000002 21.485000000000003 70-74 24.115000000000002 25.215 29.265 21.404999999999998 75-79 24.05 25.28 29.025000000000002 21.645 80-84 24.075 25.145 29.225 21.555 85-89 24.18 25.77 28.349999999999998 21.7 90-94 23.32 25.335 29.630000000000003 21.715 95-99 23.755000000000003 25.36 29.425 21.46 100-104 23.93 25.019999999999996 29.604999999999997 21.445 105-109 23.990000000000002 25.77 28.77 21.47 110-114 23.655 25.324999999999996 28.65 22.37 115-119 24.205 25.765 28.845 21.185000000000002 120-124 23.885 25.650000000000002 28.92 21.545 125-129 23.01 25.590000000000003 29.65 21.75 130-134 23.400000000000002 26.155 29.099999999999998 21.345 135-139 24.905 25.72 28.585 20.79 140-144 24.295 25.53 29.62 20.555 145-149 24.18 26.19 28.794999999999998 20.835 150 23.65 25.0 30.525000000000002 20.825 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 2.0 21 3.0 22 2.5 23 1.5 24 3.5 25 7.5 26 13.0 27 19.0 28 22.5 29 31.0 30 42.5 31 42.5 32 39.0 33 42.0 34 56.0 35 81.5 36 122.0 37 179.0 38 209.5 39 201.5 40 200.0 41 216.0 42 214.0 43 203.5 44 226.5 45 220.5 46 191.5 47 163.0 48 137.0 49 114.0 50 94.5 51 78.5 52 50.5 53 40.5 54 40.5 55 47.0 56 41.5 57 38.0 58 37.0 59 33.5 60 46.5 61 42.5 62 25.0 63 31.0 64 38.5 65 41.5 66 36.5 67 27.0 68 24.5 69 20.0 70 22.0 71 20.0 72 17.0 73 16.0 74 13.5 75 14.0 76 12.5 77 11.5 78 9.5 79 5.0 80 4.0 81 4.0 82 2.0 83 1.0 84 0.0 85 1.0 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.375 #Duplication Level Percentage of deduplicated Percentage of total 1 81.93126022913258 62.575 2 13.2569558101473 20.25 3 2.1603927986906712 4.95 4 0.9492635024549919 2.9000000000000004 5 0.6219312602291326 2.375 6 0.32733224222585927 1.5 7 0.2618657937806874 1.4000000000000001 8 0.22913256955810146 1.4000000000000001 9 0.03273322422258593 0.22499999999999998 >10 0.22913256955810146 2.4250000000000003 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 18 0.44999999999999996 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 18 0.44999999999999996 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 14 0.35000000000000003 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 14 0.35000000000000003 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 13 0.325 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 10 0.25 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 10 0.25 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 9 0.22499999999999998 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 8 0.2 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 8 0.2 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 8 0.2 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 8 0.2 No Hit GAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTC 8 0.2 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 8 0.2 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 8 0.2 No Hit GAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAA 7 0.17500000000000002 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 7 0.17500000000000002 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 7 0.17500000000000002 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 7 0.17500000000000002 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 7 0.17500000000000002 No Hit TGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATT 7 0.17500000000000002 No Hit CACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTAT 7 0.17500000000000002 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 7 0.17500000000000002 No Hit CTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGT 6 0.15 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 6 0.15 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 6 0.15 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 6 0.15 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 6 0.15 No Hit TATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGT 6 0.15 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 6 0.15 No Hit AACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGG 6 0.15 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 6 0.15 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 6 0.15 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 5 0.125 No Hit CTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGA 5 0.125 No Hit AAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATC 5 0.125 No Hit TATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACA 5 0.125 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 5 0.125 No Hit GACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCC 5 0.125 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 5 0.125 No Hit ATTAGTTGATAAGTGATAGGATCCCTTTTTTGACGCCCCCATGTCCCCCC 5 0.125 No Hit TTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAAC 5 0.125 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 5 0.125 No Hit AGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAA 5 0.125 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 5 0.125 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 5 0.125 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 5 0.125 No Hit TCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACT 5 0.125 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 5 0.125 No Hit GTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACC 5 0.125 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 5 0.125 No Hit ATTTGAAGAGGGTTCCGTTACTAACATGTTTACTTCCATTGTAGGTAACG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0125 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.05 0.0 0.0 0.0 0.0 84-85 0.05 0.0 0.0 0.0 0.0 86-87 0.0875 0.0 0.0 0.0 0.0 88-89 0.125 0.0 0.0 0.0 0.0 90-91 0.15 0.0 0.0 0.0 0.0 92-93 0.1875 0.0 0.0 0.0 0.0 94-95 0.225 0.0 0.0 0.0 0.0 96-97 0.225 0.0 0.0 0.0 0.0 98-99 0.35 0.0 0.0 0.0 0.0 100-101 0.4125 0.0 0.0 0.0 0.0 102-103 0.45 0.0 0.0 0.0 0.0 104-105 0.5625 0.0 0.0 0.0 0.0 106-107 0.625 0.0 0.0 0.0 0.0 108-109 0.675 0.0 0.0 0.0 0.0 110-111 0.825 0.0 0.0 0.0 0.0 112-113 0.8875 0.0 0.0 0.0 0.0 114-115 0.9375 0.0 0.0 0.0 0.0 116-117 1.075 0.0 0.0 0.0 0.0 118-119 1.2374999999999998 0.0 0.0 0.0 0.0 120-121 1.35 0.0 0.0 0.0 0.0 122-123 1.4874999999999998 0.0 0.0 0.0 0.0 124-125 1.8375 0.0 0.0 0.0 0.0 126-127 2.0 0.0 0.0 0.0 0.0 128-129 2.3625 0.0 0.0 0.0 0.0 130-131 2.5250000000000004 0.0 0.0 0.0 0.0 132-133 2.7125000000000004 0.0 0.0 0.0 0.0 134-135 2.9125 0.0 0.0 0.0 0.0 136-137 3.375 0.0 0.0 0.0 0.0 138 3.7 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTAACT 10 0.006973645 144.0 7 GCAACTA 10 0.006973645 144.0 4 CTAACTG 10 0.006973645 144.0 8 AACTAAC 10 0.006973645 144.0 6 >>END_MODULE Read 1626674 spots for ERR11006588.sra Written 1626674 spots for ERR11006588.sra Read 1626674 spots for ERR11006588.sra Written 1626674 spots for ERR11006588.sra Read 1626674 spots for ERR11006588.sra Written 1626674 spots for ERR11006588.sra Read 1626674 spots for ERR11006588.sra Written 1626674 spots for ERR11006588.sra Read 1626674 spots for ERR11006588.sra Written 1626674 spots for ERR11006588.sra Read 1626674 spots for ERR11006588.sra Written 1626674 spots for ERR11006588.sra Read 1626674 spots for ERR11006588.sra Written 1626674 spots for ERR11006588.sra Read 1626674 spots for ERR11006588.sra Written 1626674 spots for ERR11006588.sra Read 1626687 spots for ERR11006588.sra Written 1626687 spots for ERR11006588.sra Read 1626674 spots for ERR11006588.sra Written 1626674 spots for ERR11006588.sra Read 1626674 spots for ERR11006588.sra Written 1626674 spots for ERR11006588.sra Read 1626674 spots for ERR11006588.sra Written 1626674 spots for ERR11006588.sra Read 1626674 spots for ERR11006588.sra Written 1626674 spots for ERR11006588.sra Read 1626674 spots for ERR11006588.sra Written 1626674 spots for ERR11006588.sra Read 1626674 spots for ERR11006588.sra Written 1626674 spots for ERR11006588.sra Read 1626674 spots for ERR11006588.sra Written 1626674 spots for ERR11006588.sra Read 1626674 spots for ERR11006588.sra Written 1626674 spots for ERR11006588.sra Read 1626674 spots for ERR11006588.sra Written 1626674 spots for ERR11006588.sra Read 1626674 spots for ERR11006588.sra Written 1626674 spots for ERR11006588.sra Read 1626674 spots for ERR11006588.sra Written 1626674 spots for ERR11006588.sra SRR ids: ['ERR11006588.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_aakn7ane ERR11006588.sra spots: 32533493 blocks: [[1, 1626674], [1626675, 3253348], [3253349, 4880022], [4880023, 6506696], [6506697, 8133370], [8133371, 9760044], [9760045, 11386718], [11386719, 13013392], [13013393, 14640066], [14640067, 16266740], [16266741, 17893414], [17893415, 19520088], [19520089, 21146762], [21146763, 22773436], [22773437, 24400110], [24400111, 26026784], [26026785, 27653458], [27653459, 29280132], [29280133, 30906806], [30906807, 32533493]] ERR11006588 file size 11949869 ERR11006588 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006588 ERR11006588_1.fastq ERR11006588_2.fastq Input file: ERR11006588_1.fastq Paired file: ERR11006588_2.fastq trimmed: ERR11006588-trimmed-pair1.fastq, ERR11006588-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 18:21:38 2024 >> started Fri Dec 6 18:22:25 2024 >> done (46.286s) 32533493 read pairs processed; of these: 305 ( 0.00%) short read pairs filtered out after trimming by size control 1229 ( 0.00%) empty read pairs filtered out after trimming by size control 32531959 (100.00%) read pairs available; of these: 1929121 ( 5.93%) trimmed read pairs available after processing 30602838 (94.07%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 11 0.00% 19 9 0.00% 20 16 0.00% 21 23 0.00% 22 16 0.00% 23 13 0.00% 24 18 0.00% 25 1640 0.01% 26 21 0.00% 27 15 0.00% 28 13 0.00% 29 28 0.00% 30 27 0.00% 31 54 0.00% 32 27 0.00% 33 14 0.00% 34 16 0.00% 35 24 0.00% 36 18 0.00% 37 28 0.00% 38 30 0.00% 39 25 0.00% 40 36 0.00% 41 35 0.00% 42 35 0.00% 43 28 0.00% 44 41 0.00% 45 55 0.00% 46 40 0.00% 47 49 0.00% 48 60 0.00% 49 52 0.00% 50 75 0.00% 51 86 0.00% 52 112 0.00% 53 134 0.00% 54 122 0.00% 55 255 0.00% 56 135 0.00% 57 200 0.00% 58 192 0.00% 59 229 0.00% 60 285 0.00% 61 337 0.00% 62 343 0.00% 63 408 0.00% 64 457 0.00% 65 485 0.00% 66 546 0.00% 67 550 0.00% 68 644 0.00% 69 661 0.00% 70 726 0.00% 71 851 0.00% 72 954 0.00% 73 1123 0.00% 74 1391 0.00% 75 1517 0.00% 76 1648 0.01% 77 1721 0.01% 78 1999 0.01% 79 2027 0.01% 80 2131 0.01% 81 2441 0.01% 82 2578 0.01% 83 2904 0.01% 84 3342 0.01% 85 3866 0.01% 86 4299 0.01% 87 4475 0.01% 88 4836 0.01% 89 5209 0.02% 90 5216 0.02% 91 5429 0.02% 92 5824 0.02% 93 6405 0.02% 94 6659 0.02% 95 7396 0.02% 96 8254 0.03% 97 8863 0.03% 98 9228 0.03% 99 9589 0.03% 100 10083 0.03% 101 10487 0.03% 102 11057 0.03% 103 11605 0.04% 104 12087 0.04% 105 13242 0.04% 106 14215 0.04% 107 15029 0.05% 108 15774 0.05% 109 16600 0.05% 110 17305 0.05% 111 17886 0.05% 112 19411 0.06% 113 18965 0.06% 114 20362 0.06% 115 21337 0.07% 116 23961 0.07% 117 24157 0.07% 118 24735 0.08% 119 26502 0.08% 120 28099 0.09% 121 28795 0.09% 122 29830 0.09% 123 31409 0.10% 124 32735 0.10% 125 34519 0.11% 126 34388 0.11% 127 34647 0.11% 128 36626 0.11% 129 38684 0.12% 130 41479 0.13% 131 43675 0.13% 132 43098 0.13% 133 42901 0.13% 134 44890 0.14% 135 45671 0.14% 136 47091 0.14% 137 48596 0.15% 138 51001 0.16% 139 54722 0.17% 140 56582 0.17% 141 59457 0.18% 142 61071 0.19% 143 62258 0.19% 144 63132 0.19% 145 66739 0.21% 146 69848 0.21% 147 70627 0.22% 148 74182 0.23% 149 75895 0.23% 150 30602838 94.07% 32531959 reads passed initial QC criterion=sequence-density sequence-density=0.52 sequence-density-rank=1 fanout-score=2.78 fanout-score-rank=26 prefix-density=0.59 prefix-fanout=2.5 sequence=TCCTCTTCCCCA criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=89.61 fanout-score-rank=1 prefix-density=0.55 prefix-fanout=1.1 sequence=ATACAGAATGGTAAAGAGGGCTGTTCTACGATCATCAATTCAAGAAATCCATACAAATACGAAAGGGTATTTTATCCTTACCAACTGGATCTTGTTGCACCCGGTAACAAACATTCATAAACCATTTCTCGAAGTACGTGCCCGGATAGCCCAAAATCTCGATAGTTAGCTCTAGGTCTTCCAGTCAAAAAACAACGTCGATGAAGGCGTGTAGGTGCACTATTACGTGGTAGGGATTGCAATTTTTCTCGCATTTTCGTTTTTTCACTCAAACTCAAGGGGGAAACTTTGCTTCTTATC criterion=sequence-density sequence-density=0.59 sequence-density-rank=1 fanout-score=4.79 fanout-score-rank=18 prefix-density=0.96 prefix-fanout=2.9 sequence=TGGGGAAGAGGA criterion=fanout-score sequence-density=0.04 sequence-density-rank=31 fanout-score=143.19 fanout-score-rank=1 prefix-density=5.09 prefix-fanout=1.0 sequence=TGCATGGTTCCAGATAATCCGCTTGGTTGCAAAAAATTGTTAGAAATGCGTATGAATATACAACTTAGAGTTGTAGGACTTAGAATCCGATTCAATAGAAAATGAGAAAATACGCAAACCAAATAGAAGAAACAGATGTATATAGGATATTATATATTCCTAAGTTAGATTGATTATCTAATCCGATCTATCGAATTCCCTCTATGTAGTTCGGACAATTCACATTATCTTTTCAATTTGTACTTTTTAGTTACTTCTCCCCAATAGAGCTTAGAAGTAAGAATTTCTTGGTTGATTGTATCCTTAACCATTTCTTTTTTTTGACACGAGGAACTCACCATGAATCCATTAATTGCTGCTGCTTCTGTTATTGCTGCTGGATTGGCCGTAGGGCTTGCTTCTATTGGGCCTGGGGTTGGTCAAGGTACTGCTGCCGGACAAGCTGTAGAAGGTATTGCGAGACAGCCAGAAGCAGAAGGTAAAATACGAGGTACTTTATTGCTTAGTCTAG ERR11006588 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 18:23:27 Started mapping on | Dec 06 18:23:28 Finished on | Dec 06 18:28:18 Mapping speed, Million of reads per hour | 403.85 Number of input reads | 32531959 Average input read length | 297 UNIQUE READS: Uniquely mapped reads number | 21741250 Uniquely mapped reads % | 66.83% Average mapped length | 296.46 Number of splices: Total | 10413457 Number of splices: Annotated (sjdb) | 9777540 Number of splices: GT/AG | 10167155 Number of splices: GC/AG | 131959 Number of splices: AT/AC | 21997 Number of splices: Non-canonical | 92346 Mismatch rate per base, % | 0.31% Deletion rate per base | 0.01% Deletion average length | 1.84 Insertion rate per base | 0.01% Insertion average length | 2.01 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 9125069 % of reads mapped to multiple loci | 28.05% Number of reads mapped to too many loci | 12542 % of reads mapped to too many loci | 0.04% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.08% % of reads unmapped: other | 1.00% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1665640 1665640 1665640 N_multimapping 9125069 9125069 9125069 N_noFeature 5465741 20101441 6610880 N_ambiguous 917290 22695 432521 UnstrandedReadsAssigned:15358219 PositiveStrandReadsAssigned:1617114 NegativeStrandReadsAssigned:14697849 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006588 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006588-trimmed-pair1.fastq ERR11006588-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 32,531,959 reads, 20,692,572 reads pseudoaligned [quant] estimated average fragment length: 221.389 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,093 rounds 52973 ERR11006588.ke.tsv 35125 ERR11006588.se.tsv 88098 total ==> ERR11006588.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 716.043 0 0 PNS24247 1044 823.611 25.865 1.76634 PNS24249 1928 1707.61 53.7007 1.76878 PNS24246 1044 823.611 25.865 1.76634 PNS24248 1044 823.611 25.865 1.76634 PNS24244 1471 1250.61 40.7043 1.83064 PNS24243 293 81.0033 0 0 KQK14069 1603 1382.61 1172.21 47.6858 KQK14071 474 255.361 141.336 31.1304 ==> ERR11006588.se.tsv <== BRADI_1g14170v3 1507 BRADI_1g53295v3 181 BRADI_1g59795v3 176 BRADI_1g07683v3 0 BRADI_1g00485v3 4 BRADI_1g20270v3 129 BRADI_1g74790v3 168 BRADI_1g09890v3 0 BRADI_1g77505v3 154 BRADI_1g48960v3 5 ERR11006588 completed mapping pipeline successfully