Starting /dee2/code/volunteer_pipeline.sh ERR11006589 current disk space = 1550532997120 free memory = 1599362424 ERR11006589 SRAfilesize 28bb4e25f0c23c19b3b1891ed892dfd2 ERR11006589.sra ERR11006589.sra file validated ERR11006589 is paired end ERR11006589 is conventional basespace ERR11006589 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006589_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.436 37.0 37.0 37.0 37.0 37.0 2 36.171 37.0 37.0 37.0 37.0 37.0 3 36.4545 37.0 37.0 37.0 37.0 37.0 4 36.56 37.0 37.0 37.0 37.0 37.0 5 36.5325 37.0 37.0 37.0 37.0 37.0 6 36.567 37.0 37.0 37.0 37.0 37.0 7 36.4835 37.0 37.0 37.0 37.0 37.0 8 36.4855 37.0 37.0 37.0 37.0 37.0 9 36.572 37.0 37.0 37.0 37.0 37.0 10-14 36.45399999999999 37.0 37.0 37.0 37.0 37.0 15-19 36.4655 37.0 37.0 37.0 37.0 37.0 20-24 36.4185 37.0 37.0 37.0 37.0 37.0 25-29 36.3836 37.0 37.0 37.0 37.0 37.0 30-34 36.359899999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.2929 37.0 37.0 37.0 37.0 37.0 40-44 36.2602 37.0 37.0 37.0 37.0 37.0 45-49 36.1965 37.0 37.0 37.0 37.0 37.0 50-54 36.1967 37.0 37.0 37.0 37.0 37.0 55-59 36.1687 37.0 37.0 37.0 37.0 37.0 60-64 36.112399999999994 37.0 37.0 37.0 37.0 37.0 65-69 36.1274 37.0 37.0 37.0 37.0 37.0 70-74 36.064499999999995 37.0 37.0 37.0 37.0 37.0 75-79 36.0105 37.0 37.0 37.0 37.0 37.0 80-84 36.03869999999999 37.0 37.0 37.0 37.0 37.0 85-89 35.9276 37.0 37.0 37.0 37.0 37.0 90-94 35.9266 37.0 37.0 37.0 37.0 37.0 95-99 35.80309999999999 37.0 37.0 37.0 37.0 37.0 100-104 35.827000000000005 37.0 37.0 37.0 37.0 37.0 105-109 35.72430000000001 37.0 37.0 37.0 37.0 37.0 110-114 35.7536 37.0 37.0 37.0 37.0 37.0 115-119 35.6753 37.0 37.0 37.0 37.0 37.0 120-124 35.6245 37.0 37.0 37.0 37.0 37.0 125-129 35.524800000000006 37.0 37.0 37.0 37.0 37.0 130-134 35.4413 37.0 37.0 37.0 37.0 37.0 135-139 35.4726 37.0 37.0 37.0 37.0 37.0 140-144 35.429899999999996 37.0 37.0 37.0 37.0 37.0 145-149 35.2456 37.0 37.0 37.0 32.2 37.0 150 35.1475 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 0.0 23 2.0 24 4.0 25 9.0 26 8.0 27 14.0 28 23.0 29 29.0 30 43.0 31 61.0 32 87.0 33 121.0 34 161.0 35 297.0 36 2798.0 37 342.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.85 14.674999999999999 13.675 33.800000000000004 2 28.790160642570285 13.980923694779115 27.660642570281123 29.56827309236948 3 23.674999999999997 17.05 26.3 32.975 4 30.125 18.125 22.45 29.299999999999997 5 28.749999999999996 24.2 23.849999999999998 23.200000000000003 6 26.3 31.0 20.25 22.45 7 17.349999999999998 28.849999999999998 35.0 18.8 8 17.549999999999997 27.55 31.724999999999998 23.175 9 19.15 25.95 33.425 21.475 10-14 21.2 29.93 26.015 22.855 15-19 21.66 28.48 26.41 23.45 20-24 22.2 27.97 26.265 23.565 25-29 22.81 27.79 25.990000000000002 23.41 30-34 22.71 28.49 26.365 22.435 35-39 22.939999999999998 28.854999999999997 25.345000000000002 22.86 40-44 21.64 28.405 26.075 23.880000000000003 45-49 22.3 28.585 25.724999999999998 23.39 50-54 22.235 29.189999999999998 25.575 23.0 55-59 22.585 27.425 25.319999999999997 24.67 60-64 22.11 28.854999999999997 25.779999999999998 23.255 65-69 22.29 28.810000000000002 25.605 23.294999999999998 70-74 22.84 28.185 25.2 23.775 75-79 23.075000000000003 27.725 25.814999999999998 23.385 80-84 22.43 27.250000000000004 25.4 24.92 85-89 22.89 27.295 25.15 24.665 90-94 22.17 27.725 26.490000000000002 23.615 95-99 22.455 27.639999999999997 24.62 25.285000000000004 100-104 22.384999999999998 28.15 25.34 24.125 105-109 22.255 28.044999999999998 25.405 24.295 110-114 23.064999999999998 26.724999999999998 25.509999999999998 24.7 115-119 22.035 28.275 25.674999999999997 24.015 120-124 21.01 27.935 26.035000000000004 25.019999999999996 125-129 21.81 28.050000000000004 25.15 24.990000000000002 130-134 22.48 28.265 25.185000000000002 24.07 135-139 22.61 28.405 24.425 24.560000000000002 140-144 23.369999999999997 27.435 24.82 24.375 145-149 22.759999999999998 28.410000000000004 24.88 23.95 150 22.25 28.749999999999996 25.424999999999997 23.575 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.0 21 1.5 22 0.5 23 2.5 24 4.5 25 5.0 26 12.0 27 15.5 28 17.0 29 23.5 30 29.0 31 32.5 32 40.0 33 50.0 34 58.5 35 75.0 36 102.5 37 154.0 38 209.0 39 202.5 40 193.0 41 217.5 42 204.5 43 187.5 44 196.5 45 191.0 46 171.5 47 148.5 48 124.0 49 103.5 50 87.0 51 75.0 52 62.5 53 59.0 54 50.0 55 51.5 56 57.0 57 44.0 58 45.5 59 57.5 60 52.5 61 40.0 62 38.5 63 43.0 64 52.5 65 54.5 66 45.5 67 39.5 68 43.5 69 36.5 70 24.0 71 26.5 72 26.0 73 19.5 74 15.5 75 13.5 76 10.5 77 9.0 78 11.5 79 10.0 80 6.5 81 5.0 82 3.0 83 2.5 84 3.0 85 2.5 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.95 #Duplication Level Percentage of deduplicated Percentage of total 1 84.99073502161828 68.8 2 11.395923409512045 18.45 3 1.8529956763434219 4.5 4 0.7720815318097591 2.5 5 0.49413218035824585 2.0 6 0.12353304508956146 0.6 7 0.12353304508956146 0.7000000000000001 8 0.030883261272390366 0.2 9 0.06176652254478073 0.44999999999999996 >10 0.1544163063619518 1.7999999999999998 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 31 0.775 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 11 0.27499999999999997 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 10 0.25 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 10 0.25 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 10 0.25 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 9 0.22499999999999998 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 9 0.22499999999999998 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 8 0.2 No Hit CGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACC 7 0.17500000000000002 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 7 0.17500000000000002 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 7 0.17500000000000002 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 7 0.17500000000000002 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 6 0.15 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 6 0.15 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 6 0.15 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 6 0.15 No Hit CAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAA 5 0.125 No Hit GCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCA 5 0.125 No Hit GAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATG 5 0.125 No Hit GAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTA 5 0.125 No Hit AGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAGGGT 5 0.125 No Hit ACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACT 5 0.125 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 5 0.125 No Hit CCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAGG 5 0.125 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 5 0.125 No Hit CCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTT 5 0.125 No Hit TCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGT 5 0.125 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 5 0.125 No Hit CACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCA 5 0.125 No Hit GGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGA 5 0.125 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 5 0.125 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.11249999999999999 0.0 0.0 0.0 0.0 84-85 0.175 0.0 0.0 0.0 0.0 86-87 0.2 0.0 0.0 0.0 0.0 88-89 0.2625 0.0 0.0 0.0 0.0 90-91 0.3 0.0 0.0 0.0 0.0 92-93 0.3 0.0 0.0 0.0 0.0 94-95 0.3875 0.0 0.0 0.0 0.0 96-97 0.4 0.0 0.0 0.0 0.0 98-99 0.42500000000000004 0.0 0.0 0.0 0.0 100-101 0.5375000000000001 0.0 0.0 0.0 0.0 102-103 0.5875 0.0 0.0 0.0 0.0 104-105 0.6875 0.0 0.0 0.0 0.0 106-107 0.7749999999999999 0.0 0.0 0.0 0.0 108-109 0.8875 0.0 0.0 0.0 0.0 110-111 0.9624999999999999 0.0 0.0 0.0 0.0 112-113 1.0875 0.0 0.0 0.0 0.0 114-115 1.25 0.0 0.0 0.0 0.0 116-117 1.3875000000000002 0.0 0.0 0.0 0.0 118-119 1.4625 0.0 0.0 0.0 0.0 120-121 1.5 0.0 0.0 0.0 0.0 122-123 1.7375 0.0 0.0 0.0 0.0 124-125 1.9 0.0 0.0 0.0 0.0 126-127 2.125 0.0 0.0 0.0 0.0 128-129 2.375 0.0 0.0 0.0 0.0 130-131 2.6625 0.0 0.0 0.0 0.0 132-133 2.875 0.0 0.0 0.0 0.0 134-135 3.1375 0.0 0.0 0.0 0.0 136-137 3.5 0.0 0.0 0.0 0.0 138 3.775 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGAAA 10 0.006973645 144.0 9 AGGATTA 10 0.006973645 144.0 4 TTAGGAA 10 0.006973645 144.0 8 >>END_MODULE ERR11006589 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006589_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.3215 37.0 37.0 37.0 37.0 37.0 2 36.2375 37.0 37.0 37.0 37.0 37.0 3 36.2825 37.0 37.0 37.0 37.0 37.0 4 36.285 37.0 37.0 37.0 37.0 37.0 5 36.377 37.0 37.0 37.0 37.0 37.0 6 36.2575 37.0 37.0 37.0 37.0 37.0 7 36.3155 37.0 37.0 37.0 37.0 37.0 8 36.4145 37.0 37.0 37.0 37.0 37.0 9 36.408 37.0 37.0 37.0 37.0 37.0 10-14 36.3861 37.0 37.0 37.0 37.0 37.0 15-19 36.372699999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.3134 37.0 37.0 37.0 37.0 37.0 25-29 36.277100000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.265600000000006 37.0 37.0 37.0 37.0 37.0 35-39 36.244 37.0 37.0 37.0 37.0 37.0 40-44 36.2019 37.0 37.0 37.0 37.0 37.0 45-49 36.251799999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.214999999999996 37.0 37.0 37.0 37.0 37.0 55-59 36.1991 37.0 37.0 37.0 37.0 37.0 60-64 36.1576 37.0 37.0 37.0 37.0 37.0 65-69 36.0766 37.0 37.0 37.0 37.0 37.0 70-74 36.0538 37.0 37.0 37.0 37.0 37.0 75-79 36.0145 37.0 37.0 37.0 37.0 37.0 80-84 35.946600000000004 37.0 37.0 37.0 37.0 37.0 85-89 35.8981 37.0 37.0 37.0 37.0 37.0 90-94 35.9036 37.0 37.0 37.0 37.0 37.0 95-99 35.857099999999996 37.0 37.0 37.0 37.0 37.0 100-104 35.813900000000004 37.0 37.0 37.0 37.0 37.0 105-109 35.69109999999999 37.0 37.0 37.0 37.0 37.0 110-114 35.7036 37.0 37.0 37.0 37.0 37.0 115-119 35.646699999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.568400000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.4732 37.0 37.0 37.0 37.0 37.0 130-134 35.3668 37.0 37.0 37.0 29.8 37.0 135-139 35.2689 37.0 37.0 37.0 29.8 37.0 140-144 35.312400000000004 37.0 37.0 37.0 32.2 37.0 145-149 35.344300000000004 37.0 37.0 37.0 34.6 37.0 150 35.192 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 2.0 21 0.0 22 3.0 23 4.0 24 3.0 25 10.0 26 14.0 27 8.0 28 15.0 29 18.0 30 38.0 31 51.0 32 65.0 33 89.0 34 182.0 35 482.0 36 2819.0 37 196.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 31.45 25.2 15.15 28.199999999999996 2 27.025 27.175 30.349999999999998 15.45 3 21.6 30.049999999999997 29.65 18.7 4 24.825 29.675 24.05 21.45 5 26.825 31.125000000000004 24.775 17.275 6 22.875 36.15 22.725 18.25 7 20.849999999999998 22.275 36.325 20.549999999999997 8 23.150000000000002 24.575 28.275 24.0 9 23.75 22.025 30.375000000000004 23.849999999999998 10-14 24.905 27.169999999999998 26.19 21.735 15-19 24.905 26.72 26.865 21.51 20-24 24.26 25.455 28.275 22.009999999999998 25-29 24.279999999999998 25.055 28.155 22.509999999999998 30-34 24.365000000000002 25.755 27.83 22.05 35-39 24.83 25.77 27.944999999999997 21.455 40-44 24.765 25.814999999999998 27.389999999999997 22.03 45-49 24.315 25.555 28.065 22.065 50-54 25.185000000000002 25.145 27.134999999999998 22.535 55-59 24.34 25.330000000000002 27.255000000000003 23.075000000000003 60-64 23.335 25.695 28.08 22.89 65-69 23.735 25.365 28.34 22.56 70-74 24.959999999999997 25.695 27.465 21.88 75-79 24.5 25.245 28.015 22.24 80-84 25.09 25.174999999999997 27.650000000000002 22.085 85-89 24.740000000000002 25.89 26.855 22.515 90-94 24.295 25.455 27.96 22.29 95-99 24.275 25.495 27.985 22.245 100-104 24.515 25.990000000000002 28.12 21.375 105-109 25.0 25.28 27.744999999999997 21.975 110-114 24.45 26.185000000000002 27.355 22.009999999999998 115-119 24.595 25.995 27.615000000000002 21.795 120-124 24.745 25.615 27.544999999999998 22.095000000000002 125-129 24.595 25.974999999999998 27.375 22.055 130-134 24.92 26.484999999999996 27.41 21.185000000000002 135-139 25.215 25.180000000000003 27.750000000000004 21.855 140-144 24.75 26.16 26.875 22.215 145-149 24.82 25.595000000000002 26.93 22.655 150 25.074999999999996 25.525 27.425 21.975 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 1.5 19 3.0 20 1.5 21 0.5 22 1.0 23 4.0 24 6.0 25 8.0 26 12.5 27 18.0 28 22.0 29 29.0 30 40.5 31 44.0 32 51.5 33 60.0 34 73.0 35 91.0 36 121.0 37 146.0 38 162.5 39 175.5 40 180.0 41 173.5 42 168.5 43 191.0 44 211.0 45 202.0 46 175.0 47 149.0 48 130.0 49 112.5 50 87.5 51 69.5 52 52.0 53 42.0 54 46.0 55 50.5 56 49.0 57 47.5 58 46.5 59 46.5 60 55.0 61 50.5 62 35.0 63 45.0 64 57.5 65 49.0 66 41.0 67 33.0 68 35.5 69 45.0 70 41.0 71 33.0 72 34.0 73 31.0 74 22.5 75 17.5 76 17.0 77 16.5 78 11.0 79 7.0 80 4.5 81 4.0 82 3.5 83 2.0 84 2.0 85 1.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.25 #Duplication Level Percentage of deduplicated Percentage of total 1 84.86153846153847 68.95 2 11.661538461538463 18.95 3 1.6923076923076923 4.125 4 0.7076923076923077 2.3 5 0.5538461538461538 2.25 6 0.15384615384615385 0.75 7 0.12307692307692308 0.7000000000000001 8 0.0923076923076923 0.6 9 0.03076923076923077 0.22499999999999998 >10 0.12307692307692308 1.15 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 13 0.325 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 12 0.3 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 11 0.27499999999999997 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 10 0.25 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 9 0.22499999999999998 No Hit CTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGA 8 0.2 No Hit AACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGG 8 0.2 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 8 0.2 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 7 0.17500000000000002 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 7 0.17500000000000002 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 7 0.17500000000000002 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 7 0.17500000000000002 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 6 0.15 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 6 0.15 No Hit GGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGGCCTGGTCTG 6 0.15 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 6 0.15 No Hit GAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTT 6 0.15 No Hit GTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTC 5 0.125 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 5 0.125 No Hit TCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGA 5 0.125 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 5 0.125 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 5 0.125 No Hit CCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGA 5 0.125 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 5 0.125 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 5 0.125 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 5 0.125 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 5 0.125 No Hit CTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGT 5 0.125 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 5 0.125 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 5 0.125 No Hit CATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGA 5 0.125 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 5 0.125 No Hit AATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTA 5 0.125 No Hit TGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACT 5 0.125 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.1 0.0 0.0 0.0 0.0 84-85 0.175 0.0 0.0 0.0 0.0 86-87 0.2 0.0 0.0 0.0 0.0 88-89 0.2625 0.0 0.0 0.0 0.0 90-91 0.3 0.0 0.0 0.0 0.0 92-93 0.3 0.0 0.0 0.0 0.0 94-95 0.3875 0.0 0.0 0.0 0.0 96-97 0.4 0.0 0.0 0.0 0.0 98-99 0.42500000000000004 0.0 0.0 0.0 0.0 100-101 0.5375000000000001 0.0 0.0 0.0 0.0 102-103 0.5875 0.0 0.0 0.0 0.0 104-105 0.6875 0.0 0.0 0.0 0.0 106-107 0.7749999999999999 0.0 0.0 0.0 0.0 108-109 0.8875 0.0 0.0 0.0 0.0 110-111 0.9624999999999999 0.0 0.0 0.0 0.0 112-113 1.1 0.0 0.0 0.0 0.0 114-115 1.25 0.0 0.0 0.0 0.0 116-117 1.3875000000000002 0.0 0.0 0.0 0.0 118-119 1.4875 0.0 0.0 0.0 0.0 120-121 1.525 0.0 0.0 0.0 0.0 122-123 1.7625 0.0 0.0 0.0 0.0 124-125 1.925 0.0 0.0 0.0 0.0 126-127 2.1500000000000004 0.0 0.0 0.0 0.0 128-129 2.4 0.0 0.0 0.0 0.0 130-131 2.7125 0.0 0.0 0.0 0.0 132-133 2.925 0.0 0.0 0.0 0.0 134-135 3.1875 0.0 0.0 0.0 0.0 136-137 3.5375 0.0 0.0 0.0 0.0 138 3.8 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGGAA 10 0.006973645 144.0 5 >>END_MODULE Read 2335643 spots for ERR11006589.sra Written 2335643 spots for ERR11006589.sra Read 2335643 spots for ERR11006589.sra Written 2335643 spots for ERR11006589.sra Read 2335643 spots for ERR11006589.sra Written 2335643 spots for ERR11006589.sra Read 2335643 spots for ERR11006589.sra Written 2335643 spots for ERR11006589.sra Read 2335643 spots for ERR11006589.sra Written 2335643 spots for ERR11006589.sra Read 2335643 spots for ERR11006589.sra Written 2335643 spots for ERR11006589.sra Read 2335643 spots for ERR11006589.sra Written 2335643 spots for ERR11006589.sra Read 2335643 spots for ERR11006589.sra Written 2335643 spots for ERR11006589.sra Read 2335643 spots for ERR11006589.sra Written 2335643 spots for ERR11006589.sra Read 2335643 spots for ERR11006589.sra Written 2335643 spots for ERR11006589.sra Read 2335643 spots for ERR11006589.sra Written 2335643 spots for ERR11006589.sra Read 2335643 spots for ERR11006589.sra Written 2335643 spots for ERR11006589.sra Read 2335643 spots for ERR11006589.sra Written 2335643 spots for ERR11006589.sra Read 2335643 spots for ERR11006589.sra Written 2335643 spots for ERR11006589.sra Read 2335657 spots for ERR11006589.sra Written 2335657 spots for ERR11006589.sra Read 2335643 spots for ERR11006589.sra Written 2335643 spots for ERR11006589.sra Read 2335643 spots for ERR11006589.sra Written 2335643 spots for ERR11006589.sra Read 2335643 spots for ERR11006589.sra Written 2335643 spots for ERR11006589.sra Read 2335643 spots for ERR11006589.sra Written 2335643 spots for ERR11006589.sra Read 2335643 spots for ERR11006589.sra Written 2335643 spots for ERR11006589.sra SRR ids: ['ERR11006589.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_zjtt419p ERR11006589.sra spots: 46712874 blocks: [[1, 2335643], [2335644, 4671286], [4671287, 7006929], [7006930, 9342572], [9342573, 11678215], [11678216, 14013858], [14013859, 16349501], [16349502, 18685144], [18685145, 21020787], [21020788, 23356430], [23356431, 25692073], [25692074, 28027716], [28027717, 30363359], [30363360, 32699002], [32699003, 35034645], [35034646, 37370288], [37370289, 39705931], [39705932, 42041574], [42041575, 44377217], [44377218, 46712874]] ERR11006589 file size 17162827 ERR11006589 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006589 ERR11006589_1.fastq ERR11006589_2.fastq Input file: ERR11006589_1.fastq Paired file: ERR11006589_2.fastq trimmed: ERR11006589-trimmed-pair1.fastq, ERR11006589-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 18:24:33 2024 >> started Fri Dec 6 18:25:32 2024 >> done (58.162s) 46712874 read pairs processed; of these: 565 ( 0.00%) short read pairs filtered out after trimming by size control 1287 ( 0.00%) empty read pairs filtered out after trimming by size control 46711022 (100.00%) read pairs available; of these: 2741155 ( 5.87%) trimmed read pairs available after processing 43969867 (94.13%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 43 0.00% 19 28 0.00% 20 24 0.00% 21 42 0.00% 22 38 0.00% 23 23 0.00% 24 31 0.00% 25 2487 0.01% 26 35 0.00% 27 32 0.00% 28 27 0.00% 29 59 0.00% 30 37 0.00% 31 87 0.00% 32 47 0.00% 33 35 0.00% 34 44 0.00% 35 42 0.00% 36 56 0.00% 37 51 0.00% 38 31 0.00% 39 45 0.00% 40 62 0.00% 41 53 0.00% 42 53 0.00% 43 69 0.00% 44 61 0.00% 45 89 0.00% 46 70 0.00% 47 90 0.00% 48 79 0.00% 49 97 0.00% 50 110 0.00% 51 156 0.00% 52 174 0.00% 53 206 0.00% 54 200 0.00% 55 385 0.00% 56 235 0.00% 57 279 0.00% 58 331 0.00% 59 353 0.00% 60 353 0.00% 61 418 0.00% 62 474 0.00% 63 601 0.00% 64 613 0.00% 65 701 0.00% 66 762 0.00% 67 805 0.00% 68 848 0.00% 69 967 0.00% 70 1096 0.00% 71 1174 0.00% 72 1342 0.00% 73 1488 0.00% 74 1727 0.00% 75 2072 0.00% 76 2210 0.00% 77 2364 0.01% 78 2501 0.01% 79 2628 0.01% 80 3011 0.01% 81 3206 0.01% 82 3424 0.01% 83 3825 0.01% 84 4245 0.01% 85 5047 0.01% 86 5629 0.01% 87 6048 0.01% 88 6291 0.01% 89 6794 0.01% 90 6887 0.01% 91 7417 0.02% 92 7691 0.02% 93 8731 0.02% 94 8820 0.02% 95 10277 0.02% 96 10846 0.02% 97 11783 0.03% 98 12196 0.03% 99 12956 0.03% 100 13397 0.03% 101 14168 0.03% 102 14860 0.03% 103 15887 0.03% 104 16976 0.04% 105 18150 0.04% 106 19819 0.04% 107 20894 0.04% 108 21252 0.05% 109 22448 0.05% 110 24233 0.05% 111 24701 0.05% 112 26138 0.06% 113 26728 0.06% 114 28402 0.06% 115 29732 0.06% 116 32502 0.07% 117 33498 0.07% 118 34558 0.07% 119 37105 0.08% 120 39061 0.08% 121 40588 0.09% 122 42261 0.09% 123 43554 0.09% 124 45508 0.10% 125 48023 0.10% 126 48461 0.10% 127 49936 0.11% 128 52549 0.11% 129 54527 0.12% 130 58911 0.13% 131 60970 0.13% 132 61897 0.13% 133 61740 0.13% 134 64829 0.14% 135 65827 0.14% 136 68481 0.15% 137 71287 0.15% 138 74813 0.16% 139 79923 0.17% 140 81904 0.18% 141 86733 0.19% 142 89196 0.19% 143 90508 0.19% 144 92018 0.20% 145 95941 0.21% 146 99674 0.21% 147 102157 0.22% 148 107502 0.23% 149 110164 0.24% 150 43969867 94.13% 46711022 reads passed initial QC criterion=sequence-density sequence-density=0.39 sequence-density-rank=1 fanout-score=3.61 fanout-score-rank=25 prefix-density=0.49 prefix-fanout=2.9 sequence=TCCTCTTCCCCA criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=259.96 fanout-score-rank=1 prefix-density=0.36 prefix-fanout=7.9 sequence=AAAAAGAAAGGTGAAATGAAAGATACCATAACAATGTGTATCAGACTGCCTGTTTCCTTGTAGTAGGATCTCCAACTTTTTGGAATGTTCCAAACTCCACTTGAGCATCCAAATCTGGATCAATACCAGCAAAAACATCTCGGAACAAGGTTCTAGCGCCATGCCAAATGTGTCCGAAAAAGAAGAGCAAAGCAAAGGTAGCATGACCAAAAGTGAACCAACCCCTTGGACTGCTGCGAAAAACACCATCCGATTTCAAAGTAGCCCGATCTAATTCAAAAATTTCCCCTAATTGAGAACGCCTCGCATATTTTTTTACAGTAGCAGGATCAGAATAACTTACTCCATTAAGTTCGCCACCATAGAACTCTACTGTTACGCCTACTTGTTCAA criterion=sequence-density sequence-density=0.44 sequence-density-rank=1 fanout-score=4.33 fanout-score-rank=21 prefix-density=0.63 prefix-fanout=3.1 sequence=TGGGGAAGAGGA criterion=fanout-score sequence-density=0.02 sequence-density-rank=31 fanout-score=189.06 fanout-score-rank=1 prefix-density=4.33 prefix-fanout=1.0 sequence=TGCATGGTTCCAGATAATCCGCTTGGTTGCAAAAAATTGTTAGAAATGCGTATGAATATACAACTTAGAGTTGTAGGACTTAGAATCCGATTCAATAGAAAATGAGAAAATACGCAAACCAAATAGAAGAAACAGATGTATATAGGATATTATATATTCCTAAGTTAGATTGATTATCTAATCCGATCTATCGAATTCCCTCTATGTAGTTCGGACAATTCACATTATCTTTTCAATTTGTACTTTTTAGTTACTTCTCCCCAATAGAGCTTAGAAGTAAGAATTTCTTGGTTGATTGTATCCTTAACCATTTCTTTTTTTTGACACGAGGAACTCACCATGAATCCATTAATTGCTGCTGCTTCTGTTATTGCTGCTGGATTGGCCGTAGGGCTTGCTTCTATTGGGCCTGGGGTTGGTCAAGGTACTGCTGCCGGACAAGCTGTAGAAGGTATTGCGAGACAGCCAGAAGCAGAAGGTAAAATACGAGGTACTTTATTGCTTAGTCTAG ERR11006589 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 18:26:20 Started mapping on | Dec 06 18:26:21 Finished on | Dec 06 18:35:13 Mapping speed, Million of reads per hour | 316.09 Number of input reads | 46711022 Average input read length | 297 UNIQUE READS: Uniquely mapped reads number | 33261640 Uniquely mapped reads % | 71.21% Average mapped length | 296.34 Number of splices: Total | 16491541 Number of splices: Annotated (sjdb) | 15527770 Number of splices: GT/AG | 16115068 Number of splices: GC/AG | 213276 Number of splices: AT/AC | 31974 Number of splices: Non-canonical | 131223 Mismatch rate per base, % | 0.31% Deletion rate per base | 0.02% Deletion average length | 1.94 Insertion rate per base | 0.02% Insertion average length | 2.06 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 10646217 % of reads mapped to multiple loci | 22.79% Number of reads mapped to too many loci | 34337 % of reads mapped to too many loci | 0.07% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.97% % of reads unmapped: other | 0.95% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2803165 2803165 2803165 N_multimapping 10646217 10646217 10646217 N_noFeature 8727791 30064121 11233353 N_ambiguous 1257838 51430 565060 UnstrandedReadsAssigned:23276011 PositiveStrandReadsAssigned:3146089 NegativeStrandReadsAssigned:21463227 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006589 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006589-trimmed-pair1.fastq ERR11006589-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 46,711,022 reads, 27,993,219 reads pseudoaligned [quant] estimated average fragment length: 219.655 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,197 rounds 52973 ERR11006589.ke.tsv 35125 ERR11006589.se.tsv 88098 total ==> ERR11006589.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 717.663 0 0 PNS24247 1044 825.345 20.8611 1.07641 PNS24249 1928 1709.35 157.365 3.92061 PNS24246 1044 825.345 20.8611 1.07641 PNS24248 1044 825.345 20.8611 1.07641 PNS24244 1471 1252.35 66.0517 2.24613 PNS24243 293 82.07 2 1.03782 KQK14069 1603 1384.35 1819.94 55.9871 KQK14071 474 256.783 366.295 60.7492 ==> ERR11006589.se.tsv <== BRADI_1g14170v3 2436 BRADI_1g53295v3 317 BRADI_1g59795v3 278 BRADI_1g07683v3 4 BRADI_1g00485v3 11 BRADI_1g20270v3 171 BRADI_1g74790v3 270 BRADI_1g09890v3 11 BRADI_1g77505v3 192 BRADI_1g48960v3 16 ERR11006589 completed mapping pipeline successfully