Starting /dee2/code/volunteer_pipeline.sh ERR11006590 current disk space = 1550141227008 free memory = 1347151260 ERR11006590 SRAfilesize 4bcc9b4f5fca913576305b27ff25ff2a ERR11006590.sra ERR11006590.sra file validated ERR11006590 is paired end ERR11006590 is conventional basespace ERR11006590 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006590_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.358 37.0 37.0 37.0 37.0 37.0 2 36.224 37.0 37.0 37.0 37.0 37.0 3 36.314 37.0 37.0 37.0 37.0 37.0 4 36.3275 37.0 37.0 37.0 37.0 37.0 5 36.448 37.0 37.0 37.0 37.0 37.0 6 36.437 37.0 37.0 37.0 37.0 37.0 7 36.436 37.0 37.0 37.0 37.0 37.0 8 36.372 37.0 37.0 37.0 37.0 37.0 9 36.381 37.0 37.0 37.0 37.0 37.0 10-14 36.4365 37.0 37.0 37.0 37.0 37.0 15-19 36.414699999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.38590000000001 37.0 37.0 37.0 37.0 37.0 25-29 36.3532 37.0 37.0 37.0 37.0 37.0 30-34 36.2799 37.0 37.0 37.0 37.0 37.0 35-39 36.285900000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.233000000000004 37.0 37.0 37.0 37.0 37.0 45-49 36.2113 37.0 37.0 37.0 37.0 37.0 50-54 36.2432 37.0 37.0 37.0 37.0 37.0 55-59 36.159299999999995 37.0 37.0 37.0 37.0 37.0 60-64 36.112700000000004 37.0 37.0 37.0 37.0 37.0 65-69 36.134499999999996 37.0 37.0 37.0 37.0 37.0 70-74 36.103 37.0 37.0 37.0 37.0 37.0 75-79 36.0475 37.0 37.0 37.0 37.0 37.0 80-84 35.996300000000005 37.0 37.0 37.0 37.0 37.0 85-89 35.9535 37.0 37.0 37.0 37.0 37.0 90-94 35.8831 37.0 37.0 37.0 37.0 37.0 95-99 35.9736 37.0 37.0 37.0 37.0 37.0 100-104 35.870599999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.81529999999999 37.0 37.0 37.0 37.0 37.0 110-114 35.7111 37.0 37.0 37.0 37.0 37.0 115-119 35.7174 37.0 37.0 37.0 37.0 37.0 120-124 35.7277 37.0 37.0 37.0 37.0 37.0 125-129 35.5807 37.0 37.0 37.0 37.0 37.0 130-134 35.46470000000001 37.0 37.0 37.0 37.0 37.0 135-139 35.437400000000004 37.0 37.0 37.0 37.0 37.0 140-144 35.194599999999994 37.0 37.0 37.0 27.4 37.0 145-149 35.2 37.0 37.0 37.0 32.2 37.0 150 35.23 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 1.0 21 1.0 22 0.0 23 1.0 24 2.0 25 7.0 26 5.0 27 12.0 28 30.0 29 41.0 30 51.0 31 60.0 32 68.0 33 111.0 34 180.0 35 331.0 36 2749.0 37 349.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.4 13.850000000000001 16.55 33.2 2 30.55 10.725 26.075 32.65 3 24.65 13.900000000000002 25.275 36.175000000000004 4 28.225 15.875 23.175 32.725 5 30.0 20.9 23.775 25.324999999999996 6 27.0 29.125 19.05 24.825 7 19.375 27.625 33.475 19.525000000000002 8 17.275 29.225 31.874999999999996 21.625 9 20.125 27.150000000000002 32.925 19.8 10-14 21.685 30.98 25.779999999999998 21.555 15-19 22.375 29.03 25.419999999999998 23.175 20-24 22.455 27.67 25.474999999999998 24.4 25-29 22.91 29.45 24.51 23.13 30-34 23.419999999999998 29.770000000000003 23.34 23.47 35-39 23.26 29.604999999999997 24.81 22.325 40-44 22.42 29.304999999999996 25.124999999999996 23.150000000000002 45-49 22.855 28.095 25.82 23.23 50-54 22.49 29.299999999999997 25.615 22.595000000000002 55-59 22.155 27.925 24.759999999999998 25.16 60-64 20.805 29.345 26.31 23.54 65-69 22.495 27.839999999999996 25.81 23.855 70-74 22.770000000000003 28.825 23.755000000000003 24.65 75-79 23.27 28.244999999999997 25.264999999999997 23.22 80-84 23.990000000000002 27.584999999999997 24.485 23.94 85-89 22.545 27.894999999999996 25.28 24.279999999999998 90-94 21.725 28.565 25.165 24.545 95-99 22.61 28.115000000000002 24.279999999999998 24.995 100-104 22.555 29.354999999999997 24.060000000000002 24.03 105-109 22.095000000000002 28.23 25.335 24.34 110-114 22.814999999999998 27.1 25.319999999999997 24.765 115-119 22.23 28.46 24.545 24.765 120-124 20.945 27.975 24.755 26.325 125-129 21.575 29.459999999999997 24.13 24.834999999999997 130-134 22.900000000000002 28.845 23.93 24.325 135-139 23.925 28.815 23.185 24.075 140-144 23.735 27.735 25.1 23.43 145-149 22.62 28.825 25.040000000000003 23.515 150 23.575 26.025 27.250000000000004 23.150000000000002 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 0.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 1.5 21 2.0 22 2.5 23 3.0 24 3.5 25 4.0 26 5.5 27 5.0 28 11.0 29 15.5 30 24.0 31 31.5 32 28.5 33 33.5 34 39.0 35 63.5 36 93.0 37 140.0 38 203.5 39 203.5 40 197.0 41 248.0 42 255.5 43 224.5 44 208.5 45 202.5 46 187.0 47 153.0 48 122.0 49 97.5 50 83.5 51 69.0 52 62.5 53 50.0 54 37.5 55 41.0 56 34.5 57 26.5 58 32.0 59 35.0 60 34.5 61 40.5 62 45.0 63 50.0 64 72.5 65 95.5 66 73.0 67 39.0 68 33.0 69 29.0 70 25.5 71 24.5 72 23.0 73 22.0 74 21.0 75 19.0 76 16.5 77 14.0 78 12.0 79 9.5 80 6.5 81 3.5 82 2.0 83 1.0 84 0.0 85 1.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.05000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 82.34301147873059 60.975 2 12.727886563133017 18.85 3 2.363268062120189 5.25 4 0.7427413909520594 2.1999999999999997 5 0.5064145847400405 1.875 6 0.1350438892640108 0.6 7 0.337609723160027 1.7500000000000002 8 0.1688048615800135 1.0 9 0.1350438892640108 0.8999999999999999 >10 0.5401755570560433 6.6000000000000005 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 34 0.8500000000000001 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 25 0.625 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 25 0.625 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 20 0.5 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 19 0.475 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 19 0.475 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 15 0.375 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 14 0.35000000000000003 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 13 0.325 No Hit TCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGT 13 0.325 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 13 0.325 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 12 0.3 No Hit GCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCA 11 0.27499999999999997 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 11 0.27499999999999997 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 10 0.25 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 10 0.25 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 9 0.22499999999999998 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 9 0.22499999999999998 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 9 0.22499999999999998 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 9 0.22499999999999998 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 8 0.2 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 8 0.2 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 8 0.2 No Hit TGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGA 8 0.2 No Hit CAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTG 8 0.2 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 7 0.17500000000000002 No Hit TCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCG 7 0.17500000000000002 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 7 0.17500000000000002 No Hit CCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTT 7 0.17500000000000002 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 7 0.17500000000000002 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 7 0.17500000000000002 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 7 0.17500000000000002 No Hit GCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAGCTGCAA 7 0.17500000000000002 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 7 0.17500000000000002 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 7 0.17500000000000002 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 6 0.15 No Hit GAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAAC 6 0.15 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 6 0.15 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 6 0.15 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 5 0.125 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 5 0.125 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 5 0.125 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 5 0.125 No Hit ACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 5 0.125 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 5 0.125 No Hit TCCGCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGA 5 0.125 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 5 0.125 No Hit AGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATAT 5 0.125 No Hit GGGGTAGAAACTGCTGCGGTTAAGAAATTACAACCTTCCAAATAGGAACT 5 0.125 No Hit AACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTA 5 0.125 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 5 0.125 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 5 0.125 No Hit GAGCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTA 5 0.125 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.05 0.0 0.0 0.0 0.0 92-93 0.0625 0.0 0.0 0.0 0.0 94-95 0.0875 0.0 0.0 0.0 0.0 96-97 0.1125 0.0 0.0 0.0 0.0 98-99 0.15 0.0 0.0 0.0 0.0 100-101 0.15 0.0 0.0 0.0 0.0 102-103 0.15 0.0 0.0 0.0 0.0 104-105 0.15 0.0 0.0 0.0 0.0 106-107 0.16249999999999998 0.0 0.0 0.0 0.0 108-109 0.175 0.0 0.0 0.0 0.0 110-111 0.1875 0.0 0.0 0.0 0.0 112-113 0.2 0.0 0.0 0.0 0.0 114-115 0.225 0.0 0.0 0.0 0.0 116-117 0.2375 0.0 0.0 0.0 0.0 118-119 0.275 0.0 0.0 0.0 0.0 120-121 0.3375 0.0 0.0 0.0 0.0 122-123 0.4 0.0 0.0 0.0 0.0 124-125 0.44999999999999996 0.0 0.0 0.0 0.0 126-127 0.5375000000000001 0.0 0.0 0.0 0.0 128-129 0.6 0.0 0.0 0.0 0.0 130-131 0.7 0.0 0.0 0.0 0.0 132-133 0.7124999999999999 0.0 0.0 0.0 0.0 134-135 0.725 0.0 0.0 0.0 0.0 136-137 0.8 0.0 0.0 0.0 0.0 138 0.85 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE ERR11006590 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006590_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.63075 37.0 37.0 37.0 37.0 37.0 2 36.3075 37.0 37.0 37.0 37.0 37.0 3 36.2565 37.0 37.0 37.0 37.0 37.0 4 36.314 37.0 37.0 37.0 37.0 37.0 5 36.38 37.0 37.0 37.0 37.0 37.0 6 36.3085 37.0 37.0 37.0 37.0 37.0 7 36.3325 37.0 37.0 37.0 37.0 37.0 8 36.2955 37.0 37.0 37.0 37.0 37.0 9 36.344 37.0 37.0 37.0 37.0 37.0 10-14 36.35189999999999 37.0 37.0 37.0 37.0 37.0 15-19 36.366699999999994 37.0 37.0 37.0 37.0 37.0 20-24 36.343999999999994 37.0 37.0 37.0 37.0 37.0 25-29 36.270799999999994 37.0 37.0 37.0 37.0 37.0 30-34 36.2681 37.0 37.0 37.0 37.0 37.0 35-39 36.2539 37.0 37.0 37.0 37.0 37.0 40-44 36.2376 37.0 37.0 37.0 37.0 37.0 45-49 36.1721 37.0 37.0 37.0 37.0 37.0 50-54 36.147 37.0 37.0 37.0 37.0 37.0 55-59 36.1198 37.0 37.0 37.0 37.0 37.0 60-64 36.043 37.0 37.0 37.0 37.0 37.0 65-69 36.0289 37.0 37.0 37.0 37.0 37.0 70-74 36.0088 37.0 37.0 37.0 37.0 37.0 75-79 35.9322 37.0 37.0 37.0 37.0 37.0 80-84 35.9144 37.0 37.0 37.0 37.0 37.0 85-89 35.867799999999995 37.0 37.0 37.0 37.0 37.0 90-94 35.9103 37.0 37.0 37.0 37.0 37.0 95-99 35.7368 37.0 37.0 37.0 37.0 37.0 100-104 35.785900000000005 37.0 37.0 37.0 37.0 37.0 105-109 35.6077 37.0 37.0 37.0 37.0 37.0 110-114 35.6669 37.0 37.0 37.0 37.0 37.0 115-119 35.5574 37.0 37.0 37.0 37.0 37.0 120-124 35.5131 37.0 37.0 37.0 37.0 37.0 125-129 35.653000000000006 37.0 37.0 37.0 37.0 37.0 130-134 35.4482 37.0 37.0 37.0 37.0 37.0 135-139 35.2746 37.0 37.0 37.0 29.8 37.0 140-144 35.3165 37.0 37.0 37.0 29.8 37.0 145-149 35.309799999999996 37.0 37.0 37.0 32.2 37.0 150 35.053 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 0.0 14 0.0 15 0.0 16 0.0 17 1.0 18 1.0 19 1.0 20 2.0 21 2.0 22 0.0 23 4.0 24 7.0 25 11.0 26 7.0 27 8.0 28 20.0 29 33.0 30 34.0 31 48.0 32 73.0 33 96.0 34 184.0 35 442.0 36 2805.0 37 220.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.708386438949784 24.87891919449401 17.206219729798626 30.206474636757584 2 28.225 25.650000000000002 28.799999999999997 17.325 3 24.0 27.750000000000004 28.125 20.125 4 25.474999999999998 27.250000000000004 23.925 23.35 5 26.025 27.275 26.174999999999997 20.525 6 22.75 31.825 24.0 21.425 7 22.325 20.4 36.85 20.424999999999997 8 23.799999999999997 23.65 28.475 24.075 9 24.125 20.5 31.025000000000002 24.349999999999998 10-14 24.33 26.424999999999997 26.674999999999997 22.57 15-19 24.65 24.63 28.4 22.32 20-24 25.259999999999998 24.240000000000002 28.144999999999996 22.355 25-29 24.905 24.59 28.34 22.165000000000003 30-34 25.424999999999997 23.925 28.215 22.435 35-39 25.455 23.87 28.235 22.439999999999998 40-44 24.865000000000002 24.825 28.215 22.095000000000002 45-49 25.095 25.224999999999998 27.639999999999997 22.040000000000003 50-54 25.009999999999998 24.759999999999998 27.565 22.665 55-59 25.19 24.235 27.42 23.155 60-64 24.224999999999998 24.68 27.97 23.125 65-69 24.57 24.19 28.720000000000002 22.52 70-74 25.540000000000003 24.11 28.084999999999997 22.264999999999997 75-79 25.645 24.005000000000003 28.52 21.83 80-84 24.55 24.759999999999998 28.7 21.990000000000002 85-89 25.305 24.68 27.35 22.665 90-94 24.445 24.825 27.389999999999997 23.34 95-99 24.09 24.845 28.244999999999997 22.82 100-104 23.925 24.41 29.12 22.545 105-109 25.074999999999996 24.72 28.360000000000003 21.845 110-114 24.89 25.365 28.17 21.575 115-119 25.474999999999998 24.94 27.700000000000003 21.884999999999998 120-124 24.385 25.115 27.965 22.535 125-129 24.03 25.685000000000002 27.534999999999997 22.75 130-134 24.72 25.915 27.384999999999998 21.98 135-139 25.080000000000002 25.285000000000004 27.925 21.709999999999997 140-144 24.58 24.895 28.525 22.0 145-149 24.175 24.805 28.139999999999997 22.88 150 24.3 23.35 29.25 23.1 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 0.0 20 1.5 21 2.0 22 1.0 23 2.0 24 3.5 25 8.5 26 11.5 27 11.5 28 10.5 29 16.5 30 24.0 31 30.0 32 40.5 33 48.0 34 65.5 35 79.5 36 88.5 37 143.0 38 163.0 39 161.5 40 191.5 41 209.5 42 206.5 43 209.5 44 231.5 45 223.0 46 191.0 47 140.5 48 107.0 49 93.0 50 69.5 51 53.5 52 56.0 53 64.0 54 52.5 55 45.5 56 45.5 57 37.5 58 40.5 59 42.5 60 35.0 61 30.5 62 39.5 63 68.5 64 98.5 65 83.5 66 50.5 67 47.5 68 43.5 69 32.5 70 31.5 71 36.0 72 33.0 73 26.0 74 21.5 75 14.5 76 13.0 77 16.0 78 13.5 79 13.0 80 11.0 81 5.5 82 4.5 83 1.5 84 0.5 85 1.0 86 1.5 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.925 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.425 #Duplication Level Percentage of deduplicated Percentage of total 1 82.69545305855414 63.2 2 12.43048740595355 19.0 3 2.2571148184494603 5.175 4 1.1122015047432123 3.4000000000000004 5 0.49067713444553485 1.875 6 0.22898266274124962 1.05 7 0.16355904481517827 0.8750000000000001 8 0.16355904481517827 1.0 9 0.06542361792607132 0.44999999999999996 >10 0.39254170755642787 3.975 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 23 0.575 No Hit GAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCG 17 0.42500000000000004 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 16 0.4 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 14 0.35000000000000003 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 13 0.325 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 13 0.325 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 12 0.3 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 11 0.27499999999999997 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 10 0.25 No Hit GTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTC 10 0.25 No Hit AGCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTG 10 0.25 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 10 0.25 No Hit AATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTAT 9 0.22499999999999998 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 9 0.22499999999999998 No Hit TTCACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCT 8 0.2 No Hit CTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTA 8 0.2 No Hit GTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACT 8 0.2 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 8 0.2 No Hit CTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTAC 8 0.2 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 7 0.17500000000000002 No Hit ATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCAC 7 0.17500000000000002 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 7 0.17500000000000002 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 7 0.17500000000000002 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 7 0.17500000000000002 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 6 0.15 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 6 0.15 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 6 0.15 No Hit GCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGC 6 0.15 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 6 0.15 No Hit GCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGC 6 0.15 No Hit TCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACT 6 0.15 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 5 0.125 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 5 0.125 No Hit AAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAG 5 0.125 No Hit TGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCAT 5 0.125 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 5 0.125 No Hit CTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATT 5 0.125 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 5 0.125 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 5 0.125 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 5 0.125 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 5 0.125 No Hit TGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATT 5 0.125 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 5 0.125 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 5 0.125 No Hit ATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTG 5 0.125 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.05 0.0 0.0 0.0 0.0 92-93 0.0625 0.0 0.0 0.0 0.0 94-95 0.075 0.0 0.0 0.0 0.0 96-97 0.0875 0.0 0.0 0.0 0.0 98-99 0.125 0.0 0.0 0.0 0.0 100-101 0.125 0.0 0.0 0.0 0.0 102-103 0.125 0.0 0.0 0.0 0.0 104-105 0.125 0.0 0.0 0.0 0.0 106-107 0.1375 0.0 0.0 0.0 0.0 108-109 0.15 0.0 0.0 0.0 0.0 110-111 0.16249999999999998 0.0 0.0 0.0 0.0 112-113 0.175 0.0 0.0 0.0 0.0 114-115 0.2 0.0 0.0 0.0 0.0 116-117 0.21250000000000002 0.0 0.0 0.0 0.0 118-119 0.25 0.0 0.0 0.0 0.0 120-121 0.3125 0.0 0.0 0.0 0.0 122-123 0.375 0.0 0.0 0.0 0.0 124-125 0.42500000000000004 0.0 0.0 0.0 0.0 126-127 0.5125 0.0 0.0 0.0 0.0 128-129 0.5875 0.0 0.0 0.0 0.0 130-131 0.675 0.0 0.0 0.0 0.0 132-133 0.6875 0.0 0.0 0.0 0.0 134-135 0.7 0.0 0.0 0.0 0.0 136-137 0.775 0.0 0.0 0.0 0.0 138 0.825 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGGCTG 10 0.0069754543 143.9875 8 GAAGGCT 10 0.0069754543 143.9875 7 >>END_MODULE Read 2695007 spots for ERR11006590.sra Written 2695007 spots for ERR11006590.sra Read 2695007 spots for ERR11006590.sra Written 2695007 spots for ERR11006590.sra Read 2695007 spots for ERR11006590.sra Written 2695007 spots for ERR11006590.sra Read 2695007 spots for ERR11006590.sra Written 2695007 spots for ERR11006590.sra Read 2695007 spots for ERR11006590.sra Written 2695007 spots for ERR11006590.sra Read 2695007 spots for ERR11006590.sra Written 2695007 spots for ERR11006590.sra Read 2695007 spots for ERR11006590.sra Written 2695007 spots for ERR11006590.sra Read 2695007 spots for ERR11006590.sra Written 2695007 spots for ERR11006590.sra Read 2695007 spots for ERR11006590.sra Written 2695007 spots for ERR11006590.sra Read 2695007 spots for ERR11006590.sra Written 2695007 spots for ERR11006590.sra Read 2695007 spots for ERR11006590.sra Written 2695007 spots for ERR11006590.sra Read 2695007 spots for ERR11006590.sra Written 2695007 spots for ERR11006590.sra Read 2695007 spots for ERR11006590.sra Written 2695007 spots for ERR11006590.sra Read 2695023 spots for ERR11006590.sra Written 2695023 spots for ERR11006590.sra Read 2695007 spots for ERR11006590.sra Written 2695007 spots for ERR11006590.sra Read 2695007 spots for ERR11006590.sra Written 2695007 spots for ERR11006590.sra Read 2695007 spots for ERR11006590.sra Written 2695007 spots for ERR11006590.sra Read 2695007 spots for ERR11006590.sra Written 2695007 spots for ERR11006590.sra Read 2695007 spots for ERR11006590.sra Written 2695007 spots for ERR11006590.sra Read 2695007 spots for ERR11006590.sra Written 2695007 spots for ERR11006590.sra SRR ids: ['ERR11006590.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_vhlt8ftd ERR11006590.sra spots: 53900156 blocks: [[1, 2695007], [2695008, 5390014], [5390015, 8085021], [8085022, 10780028], [10780029, 13475035], [13475036, 16170042], [16170043, 18865049], [18865050, 21560056], [21560057, 24255063], [24255064, 26950070], [26950071, 29645077], [29645078, 32340084], [32340085, 35035091], [35035092, 37730098], [37730099, 40425105], [40425106, 43120112], [43120113, 45815119], [45815120, 48510126], [48510127, 51205133], [51205134, 53900156]] ERR11006590 file size 19805149 ERR11006590 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006590 ERR11006590_1.fastq ERR11006590_2.fastq Input file: ERR11006590_1.fastq Paired file: ERR11006590_2.fastq trimmed: ERR11006590-trimmed-pair1.fastq, ERR11006590-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 18:36:19 2024 >> started Fri Dec 6 18:37:46 2024 >> done (87.310s) 53900156 read pairs processed; of these: 216 ( 0.00%) short read pairs filtered out after trimming by size control 3098 ( 0.01%) empty read pairs filtered out after trimming by size control 53896842 (99.99%) read pairs available; of these: 728769 ( 1.35%) trimmed read pairs available after processing 53168073 (98.65%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 9 0.00% 19 13 0.00% 20 10 0.00% 21 6 0.00% 22 20 0.00% 23 10 0.00% 24 12 0.00% 25 36 0.00% 26 17 0.00% 27 25 0.00% 28 24 0.00% 29 34 0.00% 30 26 0.00% 31 118 0.00% 32 19 0.00% 33 18 0.00% 34 19 0.00% 35 19 0.00% 36 24 0.00% 37 28 0.00% 38 25 0.00% 39 37 0.00% 40 26 0.00% 41 28 0.00% 42 41 0.00% 43 45 0.00% 44 42 0.00% 45 46 0.00% 46 51 0.00% 47 55 0.00% 48 68 0.00% 49 59 0.00% 50 88 0.00% 51 61 0.00% 52 106 0.00% 53 107 0.00% 54 117 0.00% 55 131 0.00% 56 131 0.00% 57 138 0.00% 58 166 0.00% 59 132 0.00% 60 212 0.00% 61 221 0.00% 62 234 0.00% 63 274 0.00% 64 266 0.00% 65 316 0.00% 66 318 0.00% 67 370 0.00% 68 378 0.00% 69 435 0.00% 70 475 0.00% 71 472 0.00% 72 571 0.00% 73 629 0.00% 74 683 0.00% 75 765 0.00% 76 838 0.00% 77 878 0.00% 78 929 0.00% 79 948 0.00% 80 1036 0.00% 81 1001 0.00% 82 1104 0.00% 83 1290 0.00% 84 1410 0.00% 85 1564 0.00% 86 1642 0.00% 87 1847 0.00% 88 1872 0.00% 89 1935 0.00% 90 2143 0.00% 91 2219 0.00% 92 2328 0.00% 93 2462 0.00% 94 2618 0.00% 95 2731 0.01% 96 2848 0.01% 97 3036 0.01% 98 3208 0.01% 99 3271 0.01% 100 3422 0.01% 101 3593 0.01% 102 3813 0.01% 103 3982 0.01% 104 4275 0.01% 105 4478 0.01% 106 4843 0.01% 107 5077 0.01% 108 5483 0.01% 109 5671 0.01% 110 6043 0.01% 111 6127 0.01% 112 6564 0.01% 113 6861 0.01% 114 7068 0.01% 115 7644 0.01% 116 8230 0.02% 117 8591 0.02% 118 8619 0.02% 119 9422 0.02% 120 9833 0.02% 121 10407 0.02% 122 10670 0.02% 123 11402 0.02% 124 11503 0.02% 125 12396 0.02% 126 12302 0.02% 127 12520 0.02% 128 13343 0.02% 129 13596 0.03% 130 14681 0.03% 131 15961 0.03% 132 16005 0.03% 133 16174 0.03% 134 16443 0.03% 135 16885 0.03% 136 18014 0.03% 137 18807 0.03% 138 19309 0.04% 139 20636 0.04% 140 21186 0.04% 141 22449 0.04% 142 23659 0.04% 143 24281 0.05% 144 25702 0.05% 145 26318 0.05% 146 28213 0.05% 147 28543 0.05% 148 30580 0.06% 149 33181 0.06% 150 53168073 98.65% 53896842 reads passed initial QC criterion=sequence-density sequence-density=0.39 sequence-density-rank=1 fanout-score=1.00 fanout-score-rank=36 prefix-density=0.01 prefix-fanout=1.0 sequence=GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTTATCCTTCCACCGTTGGAAGCGGGCAGTTGTCGCTGCTCTGTGAAGCCAGCCTCACGCTGTGCCTGCCAACATTATGGGCCGCGAAGCCTAGCTTTCGCTTAAGCTCCAACGGCCCACTACGCAACTTGGAACGGGCGGGCCATCAGTAGCACACCCAGACCAGGCCCGCAGCGCTACGGCAACGTCCACACCACCCTTAAAGCCCCCACTC criterion=fanout-score sequence-density=0.03 sequence-density-rank=30 fanout-score=21.30 fanout-score-rank=1 prefix-density=0.44 prefix-fanout=1.5 sequence=TCTTGCCAATAACCACGGCCGCTGAATAAAAACATTAAACTGAAGGCCCAGACAAAATGAGCACCTAAGAAAAAAAGACCATATGCAGATAATGAAGAACCATAAGACTGAATTACTTGCGATGCCTGTGCCCACAAGAAATCTCGAAGCCACCCATTAATCGTAATGGAACTCTGTGCAAAGTTCCCCCCTGTGATATGAGTTACCACCCCTTGATCACTTATAGTACCCCAAACATCCGATTGCATTTTCCAACTGAAATGGAAAATGACTACCGAAATTGCATTGTACATCCAGAATAAACCTAAGAAAACATGATCCCAAGCAGATACTTGACATGTTCCGCCTCGCCCAGGCCCATCGCAAGGAAAGCGAAAACCGAGATTTGCTTTATCGGGTATCAAACGGGAACTGCGAGCAAATAAAACACCTTTCAAAAGTATTAATACAGTCACATGGATGGTAAATGCGTGAATGTGATGGACTAAAAAATCTGCAGTTCCTAATGGAA criterion=sequence-density sequence-density=0.40 sequence-density-rank=1 fanout-score=11.00 fanout-score-rank=8 prefix-density=4.38 prefix-fanout=1.0 sequence=TTGCGTAGTGGATCTGCTGGGGCCTATGCGAAAGCTGGGCCTCACGAATTCTATAGTGGCAGGCACCGCGTTAGGCTGGCTTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=37 fanout-score=49.72 fanout-score-rank=1 prefix-density=0.19 prefix-fanout=2.9 sequence=GTTCCTTTTAGTTTCGACTTTAGGGATAATTTTTTTCGCTATCTTCTTCCGAGAACCGCCTAAGGTTCCAACTAAAAGAGTAAAATAATTTTATGGAAGTAAGAAGTCTACCCATCTGGTAGACTTCTTACTTCCATTAGTCCCCGTGTTCTTCGAATGGATCTCTTAATTGTTGAGAGGGTTGCCCAAACGCGGTATATAAGGCATACCCAGTAAAGCTTACAAGTAAACCAGATATGGAGATGGCGACTAAAGTTGCTGTTTCCATTTTTATAGAATTTAAAGATTACAATGGATCTACAAAAAGATCGT ERR11006590 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 18:39:53 Started mapping on | Dec 06 18:39:53 Finished on | Dec 06 18:46:32 Mapping speed, Million of reads per hour | 486.29 Number of input reads | 53896842 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 41159085 Uniquely mapped reads % | 76.37% Average mapped length | 298.11 Number of splices: Total | 19749454 Number of splices: Annotated (sjdb) | 18603981 Number of splices: GT/AG | 19366162 Number of splices: GC/AG | 236510 Number of splices: AT/AC | 29034 Number of splices: Non-canonical | 117748 Mismatch rate per base, % | 0.30% Deletion rate per base | 0.02% Deletion average length | 1.68 Insertion rate per base | 0.01% Insertion average length | 2.05 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 10305258 % of reads mapped to multiple loci | 19.12% Number of reads mapped to too many loci | 26474 % of reads mapped to too many loci | 0.05% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.46% % of reads unmapped: other | 1.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2432499 2432499 2432499 N_multimapping 10305258 10305258 10305258 N_noFeature 8585721 36566753 12155238 N_ambiguous 1652423 52443 619262 UnstrandedReadsAssigned:30920941 PositiveStrandReadsAssigned:4539889 NegativeStrandReadsAssigned:28384585 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006590 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006590-trimmed-pair1.fastq ERR11006590-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 53,896,842 reads, 32,018,869 reads pseudoaligned [quant] estimated average fragment length: 261.544 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,105 rounds 52973 ERR11006590.ke.tsv 35125 ERR11006590.se.tsv 88098 total ==> ERR11006590.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 675.655 0 0 PNS24247 1044 783.456 29.5767 1.2427 PNS24249 1928 1667.46 204.918 4.04535 PNS24246 1044 783.456 29.5767 1.2427 PNS24248 1044 783.456 29.5767 1.2427 PNS24244 1471 1210.46 101.352 2.75621 PNS24243 293 56.2631 1 0.585069 KQK14069 1603 1342.46 1025.4 25.1433 KQK14071 474 215.234 155.274 23.7475 ==> ERR11006590.se.tsv <== BRADI_1g14170v3 1339 BRADI_1g53295v3 2085 BRADI_1g59795v3 433 BRADI_1g07683v3 1 BRADI_1g00485v3 16 BRADI_1g20270v3 243 BRADI_1g74790v3 495 BRADI_1g09890v3 0 BRADI_1g77505v3 165 BRADI_1g48960v3 1 ERR11006590 completed mapping pipeline successfully