Starting /dee2/code/volunteer_pipeline.sh ERR11006591 current disk space = 1550141227008 free memory = 1347206284 ERR11006591 SRAfilesize da7ac8eb7ffb47396811600a0303b114 ERR11006591.sra ERR11006591.sra file validated ERR11006591 is paired end ERR11006591 is conventional basespace ERR11006591 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006591_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.258 37.0 37.0 37.0 37.0 37.0 2 36.101 37.0 37.0 37.0 37.0 37.0 3 36.3325 37.0 37.0 37.0 37.0 37.0 4 36.3865 37.0 37.0 37.0 37.0 37.0 5 36.426 37.0 37.0 37.0 37.0 37.0 6 36.399 37.0 37.0 37.0 37.0 37.0 7 36.236 37.0 37.0 37.0 37.0 37.0 8 36.374 37.0 37.0 37.0 37.0 37.0 9 36.305 37.0 37.0 37.0 37.0 37.0 10-14 36.3909 37.0 37.0 37.0 37.0 37.0 15-19 36.4322 37.0 37.0 37.0 37.0 37.0 20-24 36.329 37.0 37.0 37.0 37.0 37.0 25-29 36.263400000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.2427 37.0 37.0 37.0 37.0 37.0 35-39 36.2257 37.0 37.0 37.0 37.0 37.0 40-44 36.2251 37.0 37.0 37.0 37.0 37.0 45-49 36.1596 37.0 37.0 37.0 37.0 37.0 50-54 36.0849 37.0 37.0 37.0 37.0 37.0 55-59 36.1332 37.0 37.0 37.0 37.0 37.0 60-64 36.104200000000006 37.0 37.0 37.0 37.0 37.0 65-69 36.10510000000001 37.0 37.0 37.0 37.0 37.0 70-74 36.062599999999996 37.0 37.0 37.0 37.0 37.0 75-79 35.980599999999995 37.0 37.0 37.0 37.0 37.0 80-84 35.9701 37.0 37.0 37.0 37.0 37.0 85-89 35.9028 37.0 37.0 37.0 37.0 37.0 90-94 35.8467 37.0 37.0 37.0 37.0 37.0 95-99 35.916 37.0 37.0 37.0 37.0 37.0 100-104 35.856700000000004 37.0 37.0 37.0 37.0 37.0 105-109 35.7676 37.0 37.0 37.0 37.0 37.0 110-114 35.654399999999995 37.0 37.0 37.0 37.0 37.0 115-119 35.5669 37.0 37.0 37.0 37.0 37.0 120-124 35.607899999999994 37.0 37.0 37.0 37.0 37.0 125-129 35.5298 37.0 37.0 37.0 37.0 37.0 130-134 35.344899999999996 37.0 37.0 37.0 34.6 37.0 135-139 35.414 37.0 37.0 37.0 34.6 37.0 140-144 35.2304 37.0 37.0 37.0 29.8 37.0 145-149 35.2851 37.0 37.0 37.0 32.2 37.0 150 35.1755 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 1.0 22 1.0 23 0.0 24 5.0 25 6.0 26 5.0 27 21.0 28 31.0 29 44.0 30 62.0 31 64.0 32 83.0 33 103.0 34 154.0 35 307.0 36 2722.0 37 389.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.675 12.950000000000001 14.799999999999999 34.575 2 30.349999999999998 12.45 25.674999999999997 31.525 3 24.375 13.55 27.075 35.0 4 28.025 15.225 23.474999999999998 33.275 5 30.625000000000004 18.975 23.724999999999998 26.674999999999997 6 26.85 28.95 19.975 24.224999999999998 7 20.225 29.275000000000002 31.4 19.1 8 20.575 27.125 29.4 22.900000000000002 9 20.775 25.374999999999996 32.2 21.65 10-14 23.26 28.74 25.435000000000002 22.564999999999998 15-19 24.29 28.005000000000003 24.125 23.580000000000002 20-24 23.14 27.42 24.215 25.224999999999998 25-29 23.674999999999997 28.825 23.580000000000002 23.919999999999998 30-34 23.53 28.945 23.080000000000002 24.445 35-39 24.505 27.839999999999996 24.015 23.64 40-44 23.544999999999998 28.365000000000002 23.915 24.175 45-49 23.630000000000003 27.255000000000003 24.69 24.425 50-54 23.7 27.785 24.15 24.365000000000002 55-59 22.335 26.36 24.5 26.805 60-64 21.325 28.084999999999997 25.55 25.040000000000003 65-69 23.525 27.750000000000004 24.12 24.605 70-74 25.09 26.465 22.67 25.775 75-79 25.145 27.16 24.240000000000002 23.455000000000002 80-84 25.040000000000003 26.174999999999997 24.065 24.72 85-89 23.655 26.055 25.55 24.740000000000002 90-94 23.355 27.005000000000003 25.0 24.64 95-99 23.755000000000003 27.015 24.325 24.905 100-104 23.64 27.43 23.580000000000002 25.35 105-109 22.305 27.115000000000002 24.15 26.43 110-114 23.825 26.205000000000002 23.990000000000002 25.979999999999997 115-119 23.16 27.575 23.86 25.405 120-124 22.195 27.975 23.244999999999997 26.584999999999997 125-129 22.585 28.115000000000002 23.71 25.590000000000003 130-134 24.195 27.894999999999996 22.925 24.985 135-139 24.64 28.125 21.985 25.25 140-144 24.02 27.495000000000005 24.39 24.095 145-149 23.119999999999997 27.05 24.6 25.230000000000004 150 24.075 24.975 24.7 26.25 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.0 5 0.5 6 0.5 7 0.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 1.0 21 2.0 22 4.5 23 4.5 24 1.5 25 1.0 26 4.0 27 7.5 28 8.5 29 13.0 30 15.0 31 22.5 32 26.0 33 30.5 34 43.5 35 55.0 36 73.0 37 123.0 38 178.5 39 188.0 40 201.5 41 226.0 42 212.5 43 187.5 44 176.0 45 175.5 46 158.0 47 141.5 48 133.0 49 98.5 50 74.0 51 61.0 52 57.5 53 59.5 54 45.0 55 32.5 56 32.0 57 33.0 58 43.5 59 51.0 60 53.5 61 60.0 62 56.0 63 62.0 64 117.5 65 146.5 66 95.0 67 52.0 68 50.0 69 47.5 70 36.5 71 34.0 72 32.0 73 24.0 74 20.5 75 19.0 76 16.0 77 16.0 78 12.5 79 10.5 80 12.0 81 7.0 82 3.0 83 5.0 84 4.5 85 1.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.45 #Duplication Level Percentage of deduplicated Percentage of total 1 83.49217154526889 61.324999999999996 2 11.300204220558204 16.6 3 2.3485364193328797 5.175 4 0.9189925119128658 2.7 5 0.5445881552076242 2.0 6 0.17018379850238255 0.75 7 0.3403675970047651 1.7500000000000002 8 0.17018379850238255 1.0 9 0.2042205582028591 1.35 >10 0.5105513955071478 7.35 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 47 1.175 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 27 0.675 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 27 0.675 No Hit GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 25 0.625 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 21 0.525 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 20 0.5 No Hit TCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCG 17 0.42500000000000004 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 17 0.42500000000000004 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 17 0.42500000000000004 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 16 0.4 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 16 0.4 No Hit TGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAG 13 0.325 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 11 0.27499999999999997 No Hit CAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTG 10 0.25 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 10 0.25 No Hit GCGGTGGTTCTTTGGAGTAGGATATGAGACCCAAGCGGGCCGGGAATGCA 9 0.22499999999999998 No Hit GCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCA 9 0.22499999999999998 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 9 0.22499999999999998 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 9 0.22499999999999998 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 9 0.22499999999999998 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 9 0.22499999999999998 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 8 0.2 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 8 0.2 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 8 0.2 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 8 0.2 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 8 0.2 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 7 0.17500000000000002 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 7 0.17500000000000002 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 7 0.17500000000000002 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 7 0.17500000000000002 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 7 0.17500000000000002 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 7 0.17500000000000002 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 7 0.17500000000000002 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 7 0.17500000000000002 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 7 0.17500000000000002 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 7 0.17500000000000002 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 6 0.15 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 6 0.15 No Hit TCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGT 6 0.15 No Hit GTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCG 6 0.15 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 6 0.15 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 5 0.125 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 5 0.125 No Hit CCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAA 5 0.125 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 5 0.125 No Hit CAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGG 5 0.125 No Hit GAACGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAG 5 0.125 No Hit CAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAA 5 0.125 No Hit GAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAAC 5 0.125 No Hit ACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 5 0.125 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 5 0.125 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 5 0.125 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 5 0.125 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 5 0.125 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 5 0.125 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 5 0.125 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.037500000000000006 0.0 0.0 0.0 0.0 88-89 0.05 0.0 0.0 0.0 0.0 90-91 0.05 0.0 0.0 0.0 0.0 92-93 0.05 0.0 0.0 0.0 0.0 94-95 0.05 0.0 0.0 0.0 0.0 96-97 0.05 0.0 0.0 0.0 0.0 98-99 0.05 0.0 0.0 0.0 0.0 100-101 0.05 0.0 0.0 0.0 0.0 102-103 0.05 0.0 0.0 0.0 0.0 104-105 0.0625 0.0 0.0 0.0 0.0 106-107 0.1 0.0 0.0 0.0 0.0 108-109 0.1375 0.0 0.0 0.0 0.0 110-111 0.15 0.0 0.0 0.0 0.0 112-113 0.16249999999999998 0.0 0.0 0.0 0.0 114-115 0.1875 0.0 0.0 0.0 0.0 116-117 0.21250000000000002 0.0 0.0 0.0 0.0 118-119 0.275 0.0 0.0 0.0 0.0 120-121 0.32499999999999996 0.0 0.0 0.0 0.0 122-123 0.3625 0.0 0.0 0.0 0.0 124-125 0.4375 0.0 0.0 0.0 0.0 126-127 0.4625 0.0 0.0 0.0 0.0 128-129 0.5125 0.0 0.0 0.0 0.0 130-131 0.575 0.0 0.0 0.0 0.0 132-133 0.65 0.0 0.0 0.0 0.0 134-135 0.7 0.0 0.0 0.0 0.0 136-137 0.8375 0.0 0.0 0.0 0.0 138 0.875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE ERR11006591 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006591_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.52925 37.0 37.0 37.0 37.0 37.0 2 36.072 37.0 37.0 37.0 37.0 37.0 3 36.2315 37.0 37.0 37.0 37.0 37.0 4 36.277 37.0 37.0 37.0 37.0 37.0 5 36.2955 37.0 37.0 37.0 37.0 37.0 6 36.2125 37.0 37.0 37.0 37.0 37.0 7 36.346 37.0 37.0 37.0 37.0 37.0 8 36.2495 37.0 37.0 37.0 37.0 37.0 9 36.324 37.0 37.0 37.0 37.0 37.0 10-14 36.271699999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.275200000000005 37.0 37.0 37.0 37.0 37.0 20-24 36.20060000000001 37.0 37.0 37.0 37.0 37.0 25-29 36.203900000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.1671 37.0 37.0 37.0 37.0 37.0 35-39 36.0963 37.0 37.0 37.0 37.0 37.0 40-44 36.1329 37.0 37.0 37.0 37.0 37.0 45-49 36.0981 37.0 37.0 37.0 37.0 37.0 50-54 36.036699999999996 37.0 37.0 37.0 37.0 37.0 55-59 36.0462 37.0 37.0 37.0 37.0 37.0 60-64 35.9419 37.0 37.0 37.0 37.0 37.0 65-69 35.9381 37.0 37.0 37.0 37.0 37.0 70-74 35.9362 37.0 37.0 37.0 37.0 37.0 75-79 35.88590000000001 37.0 37.0 37.0 37.0 37.0 80-84 35.7955 37.0 37.0 37.0 37.0 37.0 85-89 35.8067 37.0 37.0 37.0 37.0 37.0 90-94 35.8008 37.0 37.0 37.0 37.0 37.0 95-99 35.6468 37.0 37.0 37.0 37.0 37.0 100-104 35.5735 37.0 37.0 37.0 37.0 37.0 105-109 35.5189 37.0 37.0 37.0 37.0 37.0 110-114 35.542699999999996 37.0 37.0 37.0 37.0 37.0 115-119 35.4801 37.0 37.0 37.0 37.0 37.0 120-124 35.3608 37.0 37.0 37.0 34.6 37.0 125-129 35.370099999999994 37.0 37.0 37.0 37.0 37.0 130-134 35.3802 37.0 37.0 37.0 34.6 37.0 135-139 35.2342 37.0 37.0 37.0 29.8 37.0 140-144 35.148399999999995 37.0 37.0 37.0 27.4 37.0 145-149 35.193 37.0 37.0 37.0 29.8 37.0 150 35.0255 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 0.0 16 0.0 17 2.0 18 0.0 19 1.0 20 2.0 21 4.0 22 3.0 23 3.0 24 11.0 25 8.0 26 14.0 27 14.0 28 21.0 29 30.0 30 44.0 31 51.0 32 84.0 33 103.0 34 176.0 35 532.0 36 2717.0 37 179.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 31.550802139037433 24.293353705118413 15.406162464985995 28.749681690858157 2 30.9 22.825 26.575 19.7 3 23.025000000000002 28.499999999999996 28.15 20.325 4 25.650000000000002 27.575 23.474999999999998 23.3 5 26.700000000000003 27.575 25.275 20.45 6 23.325000000000003 32.550000000000004 23.325000000000003 20.8 7 22.15 22.5 35.275 20.075000000000003 8 23.95 22.7 29.075 24.275 9 22.45 21.9 32.550000000000004 23.1 10-14 25.36 25.729999999999997 25.895000000000003 23.015 15-19 25.295 24.610000000000003 27.595 22.5 20-24 26.52 23.7 26.900000000000002 22.88 25-29 24.985 24.22 28.29 22.505 30-34 26.545 23.925 27.935 21.595 35-39 26.88 23.62 27.534999999999997 21.965 40-44 25.330000000000002 24.34 27.155 23.175 45-49 25.82 24.01 27.1 23.07 50-54 25.83 23.695 26.665 23.810000000000002 55-59 25.435000000000002 23.35 26.8 24.415 60-64 25.3 23.29 27.615000000000002 23.794999999999998 65-69 26.375 23.74 26.534999999999997 23.35 70-74 27.060000000000002 22.515 27.845 22.58 75-79 25.555 23.805 27.700000000000003 22.939999999999998 80-84 25.365 23.95 27.47 23.215 85-89 25.814999999999998 24.11 25.785000000000004 24.29 90-94 25.430000000000003 23.055 26.86 24.654999999999998 95-99 24.755 24.215 27.54 23.49 100-104 25.6 24.3 27.725 22.375 105-109 25.955000000000002 23.905 27.6 22.54 110-114 25.6 24.16 27.175 23.064999999999998 115-119 25.795 24.610000000000003 26.995 22.6 120-124 24.98 24.779999999999998 26.650000000000002 23.59 125-129 24.224999999999998 25.580000000000002 26.590000000000003 23.605 130-134 25.074999999999996 25.245 26.369999999999997 23.31 135-139 25.580000000000002 23.915 27.655 22.85 140-144 25.53 24.63 27.055 22.785 145-149 25.19 23.47 27.36 23.98 150 25.3 23.35 26.6 24.75 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 1.0 19 0.5 20 0.5 21 1.0 22 3.0 23 3.5 24 2.0 25 8.0 26 13.5 27 13.5 28 15.5 29 21.0 30 31.0 31 44.0 32 44.5 33 41.0 34 48.0 35 57.0 36 88.0 37 135.5 38 155.5 39 158.0 40 172.0 41 171.5 42 175.0 43 180.5 44 186.0 45 187.0 46 171.0 47 134.5 48 99.5 49 88.5 50 75.0 51 63.0 52 57.0 53 55.0 54 50.0 55 43.5 56 36.5 57 40.0 58 51.5 59 55.0 60 51.5 61 50.5 62 53.0 63 105.0 64 135.5 65 116.0 66 81.5 67 49.0 68 45.5 69 41.0 70 41.5 71 39.0 72 34.5 73 28.5 74 25.5 75 27.0 76 21.0 77 21.0 78 19.0 79 9.5 80 7.5 81 6.5 82 3.5 83 2.5 84 3.0 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.825 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.875 #Duplication Level Percentage of deduplicated Percentage of total 1 82.97161936560936 62.125 2 10.918196994991652 16.35 3 3.2387312186978297 7.2749999999999995 4 1.2353923205342237 3.6999999999999997 5 0.5008347245409015 1.875 6 0.1335559265442404 0.6 7 0.23372287145242068 1.225 8 0.20033388981636058 1.2 9 0.0667779632721202 0.44999999999999996 >10 0.5008347245409015 5.2 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 33 0.8250000000000001 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 16 0.4 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 16 0.4 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 15 0.375 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 13 0.325 No Hit GAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCG 13 0.325 No Hit GCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGC 13 0.325 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 13 0.325 No Hit CTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCG 12 0.3 No Hit GCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGC 12 0.3 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 11 0.27499999999999997 No Hit GTGGACGTTGCCGTAGCGCTGCGGGCCTGGTCTGGGTGTGCTACTGATGG 11 0.27499999999999997 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 10 0.25 No Hit AGCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTG 10 0.25 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 10 0.25 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 9 0.22499999999999998 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 9 0.22499999999999998 No Hit CGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGGCC 8 0.2 No Hit CGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCC 8 0.2 No Hit GGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGGCCTGGTC 8 0.2 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 8 0.2 No Hit AACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGG 8 0.2 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 8 0.2 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 7 0.17500000000000002 No Hit CGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTT 7 0.17500000000000002 No Hit CCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGG 7 0.17500000000000002 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 7 0.17500000000000002 No Hit AAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAG 7 0.17500000000000002 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 7 0.17500000000000002 No Hit AGTAGCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACG 7 0.17500000000000002 No Hit GTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTG 6 0.15 No Hit GTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACT 6 0.15 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 6 0.15 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 6 0.15 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 5 0.125 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 5 0.125 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 5 0.125 No Hit GTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCT 5 0.125 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 5 0.125 No Hit CGTTGCCGTAGCGCTGCGGGCCTGGTCTGGGTGTGCTACTGATGGCCCGC 5 0.125 No Hit ATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGA 5 0.125 No Hit AGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAG 5 0.125 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 5 0.125 No Hit AATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGT 5 0.125 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 5 0.125 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 5 0.125 No Hit GCGAGAGCTTGTAACCCGAGTGGGGGCATTTAAGGTGGTGTGGACTCTGG 5 0.125 No Hit TGTGGACGTTGCCGTAGCGCTGCGGGCCTGGTCTGGGTGTGCTACTGATG 5 0.125 No Hit AGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.037500000000000006 0.0 0.0 0.0 0.0 88-89 0.05 0.0 0.0 0.0 0.0 90-91 0.05 0.0 0.0 0.0 0.0 92-93 0.05 0.0 0.0 0.0 0.0 94-95 0.05 0.0 0.0 0.0 0.0 96-97 0.05 0.0 0.0 0.0 0.0 98-99 0.05 0.0 0.0 0.0 0.0 100-101 0.05 0.0 0.0 0.0 0.0 102-103 0.05 0.0 0.0 0.0 0.0 104-105 0.0625 0.0 0.0 0.0 0.0 106-107 0.1 0.0 0.0 0.0 0.0 108-109 0.1375 0.0 0.0 0.0 0.0 110-111 0.15 0.0 0.0 0.0 0.0 112-113 0.16249999999999998 0.0 0.0 0.0 0.0 114-115 0.1875 0.0 0.0 0.0 0.0 116-117 0.21250000000000002 0.0 0.0 0.0 0.0 118-119 0.275 0.0 0.0 0.0 0.0 120-121 0.32499999999999996 0.0 0.0 0.0 0.0 122-123 0.3625 0.0 0.0 0.0 0.0 124-125 0.4375 0.0 0.0 0.0 0.0 126-127 0.4625 0.0 0.0 0.0 0.0 128-129 0.525 0.0 0.0 0.0 0.0 130-131 0.6125 0.0 0.0 0.0 0.0 132-133 0.7 0.0 0.0 0.0 0.0 134-135 0.75 0.0 0.0 0.0 0.0 136-137 0.8875 0.0 0.0 0.0 0.0 138 0.925 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCTGTT 10 0.0069754543 143.9875 7 >>END_MODULE Read 2773238 spots for ERR11006591.sra Written 2773238 spots for ERR11006591.sra Read 2773238 spots for ERR11006591.sra Written 2773238 spots for ERR11006591.sra Read 2773238 spots for ERR11006591.sra Written 2773238 spots for ERR11006591.sra Read 2773238 spots for ERR11006591.sra Written 2773238 spots for ERR11006591.sra Read 2773238 spots for ERR11006591.sra Written 2773238 spots for ERR11006591.sra Read 2773238 spots for ERR11006591.sra Written 2773238 spots for ERR11006591.sra Read 2773238 spots for ERR11006591.sra Written 2773238 spots for ERR11006591.sra Read 2773238 spots for ERR11006591.sra Written 2773238 spots for ERR11006591.sra Read 2773238 spots for ERR11006591.sra Written 2773238 spots for ERR11006591.sra Read 2773238 spots for ERR11006591.sra Written 2773238 spots for ERR11006591.sra Read 2773238 spots for ERR11006591.sra Written 2773238 spots for ERR11006591.sra Read 2773238 spots for ERR11006591.sra Written 2773238 spots for ERR11006591.sra Read 2773238 spots for ERR11006591.sra Written 2773238 spots for ERR11006591.sra Read 2773238 spots for ERR11006591.sra Written 2773238 spots for ERR11006591.sra Read 2773238 spots for ERR11006591.sra Written 2773238 spots for ERR11006591.sra Read 2773238 spots for ERR11006591.sra Written 2773238 spots for ERR11006591.sra Read 2773238 spots for ERR11006591.sra Written 2773238 spots for ERR11006591.sra Read 2773246 spots for ERR11006591.sra Written 2773246 spots for ERR11006591.sra Read 2773238 spots for ERR11006591.sra Written 2773238 spots for ERR11006591.sra Read 2773238 spots for ERR11006591.sra Written 2773238 spots for ERR11006591.sra SRR ids: ['ERR11006591.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_od_1vak_ ERR11006591.sra spots: 55464768 blocks: [[1, 2773238], [2773239, 5546476], [5546477, 8319714], [8319715, 11092952], [11092953, 13866190], [13866191, 16639428], [16639429, 19412666], [19412667, 22185904], [22185905, 24959142], [24959143, 27732380], [27732381, 30505618], [30505619, 33278856], [33278857, 36052094], [36052095, 38825332], [38825333, 41598570], [41598571, 44371808], [44371809, 47145046], [47145047, 49918284], [49918285, 52691522], [52691523, 55464768]] ERR11006591 file size 20380371 ERR11006591 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006591 ERR11006591_1.fastq ERR11006591_2.fastq Input file: ERR11006591_1.fastq Paired file: ERR11006591_2.fastq trimmed: ERR11006591-trimmed-pair1.fastq, ERR11006591-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 18:38:13 2024 >> started Fri Dec 6 18:39:36 2024 >> done (83.887s) 55464768 read pairs processed; of these: 249 ( 0.00%) short read pairs filtered out after trimming by size control 2855 ( 0.01%) empty read pairs filtered out after trimming by size control 55461664 (99.99%) read pairs available; of these: 755608 ( 1.36%) trimmed read pairs available after processing 54706056 (98.64%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 19 0.00% 19 11 0.00% 20 13 0.00% 21 5 0.00% 22 26 0.00% 23 16 0.00% 24 13 0.00% 25 43 0.00% 26 23 0.00% 27 26 0.00% 28 30 0.00% 29 44 0.00% 30 25 0.00% 31 183 0.00% 32 27 0.00% 33 48 0.00% 34 31 0.00% 35 39 0.00% 36 32 0.00% 37 34 0.00% 38 35 0.00% 39 23 0.00% 40 34 0.00% 41 46 0.00% 42 39 0.00% 43 50 0.00% 44 79 0.00% 45 57 0.00% 46 71 0.00% 47 64 0.00% 48 49 0.00% 49 90 0.00% 50 95 0.00% 51 95 0.00% 52 115 0.00% 53 130 0.00% 54 108 0.00% 55 152 0.00% 56 126 0.00% 57 169 0.00% 58 149 0.00% 59 156 0.00% 60 192 0.00% 61 213 0.00% 62 249 0.00% 63 261 0.00% 64 308 0.00% 65 369 0.00% 66 343 0.00% 67 361 0.00% 68 435 0.00% 69 388 0.00% 70 451 0.00% 71 501 0.00% 72 548 0.00% 73 598 0.00% 74 671 0.00% 75 726 0.00% 76 763 0.00% 77 756 0.00% 78 928 0.00% 79 937 0.00% 80 989 0.00% 81 1020 0.00% 82 1081 0.00% 83 1249 0.00% 84 1291 0.00% 85 1608 0.00% 86 1680 0.00% 87 1804 0.00% 88 1881 0.00% 89 2082 0.00% 90 2017 0.00% 91 2183 0.00% 92 2326 0.00% 93 2385 0.00% 94 2600 0.00% 95 2676 0.00% 96 2959 0.01% 97 2985 0.01% 98 3250 0.01% 99 3461 0.01% 100 3584 0.01% 101 3874 0.01% 102 3994 0.01% 103 3999 0.01% 104 4509 0.01% 105 4736 0.01% 106 4856 0.01% 107 5094 0.01% 108 5351 0.01% 109 5607 0.01% 110 6279 0.01% 111 6235 0.01% 112 6647 0.01% 113 7075 0.01% 114 7658 0.01% 115 7806 0.01% 116 8798 0.02% 117 9178 0.02% 118 9211 0.02% 119 9750 0.02% 120 10649 0.02% 121 10771 0.02% 122 11155 0.02% 123 11990 0.02% 124 12327 0.02% 125 12809 0.02% 126 13086 0.02% 127 12704 0.02% 128 13439 0.02% 129 13938 0.03% 130 15472 0.03% 131 15990 0.03% 132 16646 0.03% 133 16878 0.03% 134 17058 0.03% 135 17929 0.03% 136 18720 0.03% 137 19618 0.04% 138 20581 0.04% 139 21722 0.04% 140 22132 0.04% 141 23692 0.04% 142 24536 0.04% 143 25132 0.05% 144 26554 0.05% 145 26859 0.05% 146 28642 0.05% 147 29866 0.05% 148 31846 0.06% 149 34481 0.06% 150 54706056 98.64% 55461664 reads passed initial QC criterion=sequence-density sequence-density=0.64 sequence-density-rank=1 fanout-score=1.00 fanout-score-rank=39 prefix-density=0.01 prefix-fanout=1.0 sequence=GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTTATCCTTCCACCGTTGGAAGCGGGCAGTTGTCGCTGCTCTGTGAAGCCAGCCTCACGCTGTGCCTGCCAACATTATGGGCCGCGAAGCCTAGCTTTCGCTTAAGCTCCAACGGCCCACTACGCAACTTGGAACGGGCGGGCCATCAGTAGCACACCCAGACCAGGCCCGCAGCGCTACGGCAACGTCCACACCACCCTTAAAGCCCCCACTCG criterion=fanout-score sequence-density=0.01 sequence-density-rank=44 fanout-score=117.66 fanout-score-rank=1 prefix-density=0.50 prefix-fanout=1.2 sequence=GGGCCGGGACCCCCGAGCCCAGTCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCCCTTGCCTACATTGTTCCATTGGCCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGAACGGTACTCGGTCCTCCGGATTTTCATGGGCCGCCGGGGGCGCACCGGACACCGCGCGACGTGCGGTGCTCTTCCGGCCACTGGACCCTACCTCCGGCTGAACCGATTCCAGGGTTGGCAGGCCGTTAAGCAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGGCGTCTCCGGACTTCCTAACGTCGCCGTCAACCGCCACATCCCGGCTCGGGAAATCTTA criterion=sequence-density sequence-density=0.71 sequence-density-rank=1 fanout-score=10.16 fanout-score-rank=9 prefix-density=7.17 prefix-fanout=1.0 sequence=TTGCGTAGTGGATCTGCTGGGGCCTATGCGAAAGCTGGGCCTCACGAATTCTATAGTGGCAGGCACCGCGTTAGGCTGGCTTC criterion=fanout-score sequence-density=0.02 sequence-density-rank=34 fanout-score=80.12 fanout-score-rank=1 prefix-density=0.19 prefix-fanout=9.8 sequence=CCGGCGCCGCTTGGGATCACGTCGACCGGGAGGACCCGCCCGCTCTCCGTGGCCTCCGCGGCGCAGGAGAACAGGGACATCTCATCCCTGGACGTCCAAGTCAGCCAGAACGGCGGCAACCAGCAGGGCAATGCCGTCCAACGCCGCCCACGCCGCGCCGGATTTGACGTCTCCCCGTTCGGGCTAGTGGACCCGATGTCCCCGATGCGGACGATGCGGCAGATGCTGGACACGATGGACCGGCTGTTCGACGACACTGTGGGGTTCCCCACGGCGCGGGGGCGATCACCGGCGGCGAGCGAGACGCGGATGCCGTGGGACATCATGGAAGACGACAAGGAGGTGAAGATGAGGTTCGACATGCCGGGGCTTTCGCGGGAGGAGGTGA ERR11006591 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 18:40:36 Started mapping on | Dec 06 18:40:36 Finished on | Dec 06 18:48:31 Mapping speed, Million of reads per hour | 420.34 Number of input reads | 55461664 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 44498266 Uniquely mapped reads % | 80.23% Average mapped length | 298.13 Number of splices: Total | 19580821 Number of splices: Annotated (sjdb) | 18417494 Number of splices: GT/AG | 19233602 Number of splices: GC/AG | 207228 Number of splices: AT/AC | 28454 Number of splices: Non-canonical | 111537 Mismatch rate per base, % | 0.30% Deletion rate per base | 0.02% Deletion average length | 1.67 Insertion rate per base | 0.01% Insertion average length | 2.07 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 8558424 % of reads mapped to multiple loci | 15.43% Number of reads mapped to too many loci | 26711 % of reads mapped to too many loci | 0.05% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.41% % of reads unmapped: other | 0.88% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2404974 2404974 2404974 N_multimapping 8558424 8558424 8558424 N_noFeature 9061708 39560112 13038442 N_ambiguous 1480021 43704 506667 UnstrandedReadsAssigned:33956537 PositiveStrandReadsAssigned:4894450 NegativeStrandReadsAssigned:30953157 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006591 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006591-trimmed-pair1.fastq ERR11006591-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 55,461,664 reads, 31,906,941 reads pseudoaligned [quant] estimated average fragment length: 263.325 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,130 rounds 52973 ERR11006591.ke.tsv 35125 ERR11006591.se.tsv 88098 total ==> ERR11006591.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 673.964 0 0 PNS24247 1044 781.675 27.589 1.16264 PNS24249 1928 1665.68 180.885 3.57724 PNS24246 1044 781.675 27.589 1.16264 PNS24248 1044 781.675 27.589 1.16264 PNS24244 1471 1208.68 85.3478 2.32605 PNS24243 293 56.3126 1 0.584965 KQK14069 1603 1340.68 696.318 17.1088 KQK14071 474 213.385 33.8429 5.22443 ==> ERR11006591.se.tsv <== BRADI_1g14170v3 804 BRADI_1g53295v3 3114 BRADI_1g59795v3 385 BRADI_1g07683v3 3 BRADI_1g00485v3 10 BRADI_1g20270v3 274 BRADI_1g74790v3 538 BRADI_1g09890v3 1 BRADI_1g77505v3 97 BRADI_1g48960v3 6 ERR11006591 completed mapping pipeline successfully