Starting /dee2/code/volunteer_pipeline.sh ERR11006592 current disk space = 1550162657280 free memory = 1294517732 ERR11006592 SRAfilesize 17dcb0e62ba3be9d3d8d2c23da925f89 ERR11006592.sra ERR11006592.sra file validated ERR11006592 is paired end ERR11006592 is conventional basespace ERR11006592 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006592_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.3455 37.0 37.0 37.0 37.0 37.0 2 36.0885 37.0 37.0 37.0 37.0 37.0 3 36.2185 37.0 37.0 37.0 37.0 37.0 4 36.3755 37.0 37.0 37.0 37.0 37.0 5 36.3805 37.0 37.0 37.0 37.0 37.0 6 36.4135 37.0 37.0 37.0 37.0 37.0 7 36.2865 37.0 37.0 37.0 37.0 37.0 8 36.324 37.0 37.0 37.0 37.0 37.0 9 36.385 37.0 37.0 37.0 37.0 37.0 10-14 36.423500000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.4003 37.0 37.0 37.0 37.0 37.0 20-24 36.34570000000001 37.0 37.0 37.0 37.0 37.0 25-29 36.310700000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.2807 37.0 37.0 37.0 37.0 37.0 35-39 36.2187 37.0 37.0 37.0 37.0 37.0 40-44 36.1885 37.0 37.0 37.0 37.0 37.0 45-49 36.199 37.0 37.0 37.0 37.0 37.0 50-54 36.231300000000005 37.0 37.0 37.0 37.0 37.0 55-59 36.1814 37.0 37.0 37.0 37.0 37.0 60-64 36.1217 37.0 37.0 37.0 37.0 37.0 65-69 36.1074 37.0 37.0 37.0 37.0 37.0 70-74 36.0615 37.0 37.0 37.0 37.0 37.0 75-79 36.046800000000005 37.0 37.0 37.0 37.0 37.0 80-84 36.008500000000005 37.0 37.0 37.0 37.0 37.0 85-89 35.9819 37.0 37.0 37.0 37.0 37.0 90-94 35.892900000000004 37.0 37.0 37.0 37.0 37.0 95-99 35.9364 37.0 37.0 37.0 37.0 37.0 100-104 35.8572 37.0 37.0 37.0 37.0 37.0 105-109 35.8454 37.0 37.0 37.0 37.0 37.0 110-114 35.7155 37.0 37.0 37.0 37.0 37.0 115-119 35.7904 37.0 37.0 37.0 37.0 37.0 120-124 35.6457 37.0 37.0 37.0 37.0 37.0 125-129 35.591699999999996 37.0 37.0 37.0 37.0 37.0 130-134 35.468399999999995 37.0 37.0 37.0 37.0 37.0 135-139 35.456 37.0 37.0 37.0 34.6 37.0 140-144 35.183 37.0 37.0 37.0 29.8 37.0 145-149 35.2969 37.0 37.0 37.0 34.6 37.0 150 35.1875 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 0.0 22 0.0 23 0.0 24 1.0 25 5.0 26 9.0 27 19.0 28 21.0 29 36.0 30 50.0 31 66.0 32 85.0 33 111.0 34 154.0 35 364.0 36 2733.0 37 344.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.825 13.450000000000001 16.3 33.425 2 27.650000000000002 11.4 27.250000000000004 33.7 3 22.45 14.299999999999999 26.5 36.75 4 26.900000000000002 15.975 24.925 32.2 5 28.849999999999998 20.9 25.1 25.15 6 25.724999999999998 27.700000000000003 22.35 24.224999999999998 7 19.05 31.474999999999998 31.75 17.724999999999998 8 16.475 29.5 31.874999999999996 22.15 9 17.1 28.4 34.275 20.225 10-14 20.419999999999998 31.655 26.25 21.675 15-19 22.02 30.375000000000004 25.535000000000004 22.07 20-24 20.94 28.89 26.284999999999997 23.885 25-29 23.02 29.975 25.014999999999997 21.990000000000002 30-34 23.724999999999998 30.385 23.849999999999998 22.040000000000003 35-39 22.775000000000002 30.17 25.105 21.95 40-44 22.02 30.745 24.69 22.545 45-49 22.040000000000003 28.994999999999997 25.7 23.265 50-54 21.535 30.84 25.215 22.41 55-59 22.115000000000002 28.99 23.98 24.915000000000003 60-64 21.0 29.875 26.090000000000003 23.035 65-69 21.925 29.54 25.505 23.03 70-74 23.035 29.34 23.47 24.154999999999998 75-79 23.169999999999998 29.2 24.545 23.085 80-84 23.455000000000002 28.645 24.185000000000002 23.715 85-89 22.585 28.435 25.515 23.465 90-94 21.29 28.375 26.235000000000003 24.099999999999998 95-99 22.55 29.385 24.03 24.035 100-104 22.015 30.705 23.665 23.615 105-109 22.35 29.015 24.740000000000002 23.895 110-114 23.125 27.715 25.11 24.05 115-119 21.5 29.580000000000002 24.529999999999998 24.39 120-124 20.27 29.24 25.27 25.22 125-129 21.285 30.165 23.715 24.834999999999997 130-134 22.46 29.845 24.23 23.465 135-139 23.200000000000003 29.34 23.355 24.104999999999997 140-144 23.355 28.455000000000002 25.330000000000002 22.86 145-149 22.605 29.630000000000003 24.705 23.06 150 23.200000000000003 26.35 27.650000000000002 22.8 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.0 20 0.5 21 0.5 22 1.0 23 1.0 24 2.0 25 5.0 26 7.5 27 7.0 28 10.5 29 18.0 30 26.5 31 29.0 32 30.5 33 43.0 34 56.5 35 65.0 36 84.5 37 151.0 38 232.0 39 240.5 40 221.0 41 249.0 42 248.5 43 227.5 44 212.0 45 195.0 46 185.5 47 157.5 48 134.5 49 109.0 50 83.0 51 59.5 52 48.0 53 46.0 54 37.5 55 35.5 56 33.0 57 33.0 58 31.5 59 33.5 60 40.5 61 41.0 62 34.0 63 30.5 64 62.5 65 96.5 66 69.5 67 29.5 68 26.0 69 29.0 70 27.5 71 27.0 72 21.0 73 14.5 74 12.5 75 11.5 76 11.0 77 6.0 78 3.0 79 3.0 80 2.0 81 2.5 82 2.0 83 1.0 84 1.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.72500000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 82.25521385648639 58.175 2 11.770943796394485 16.650000000000002 3 2.332979851537646 4.95 4 1.025097207493814 2.9000000000000004 5 0.671615411806292 2.375 6 0.4241781548250266 1.7999999999999998 7 0.38882997525627433 1.925 8 0.07069635913750442 0.4 9 0.3181336161187699 2.025 >10 0.7069635913750442 7.324999999999999 >50 0.03534817956875221 1.4749999999999999 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 59 1.4749999999999999 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 27 0.675 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 24 0.6 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 23 0.575 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 20 0.5 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 17 0.42500000000000004 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 15 0.375 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 15 0.375 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 15 0.375 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 14 0.35000000000000003 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 12 0.3 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 12 0.3 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 12 0.3 No Hit TCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGT 12 0.3 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 12 0.3 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 11 0.27499999999999997 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 11 0.27499999999999997 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 11 0.27499999999999997 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 10 0.25 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 10 0.25 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 10 0.25 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 9 0.22499999999999998 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 9 0.22499999999999998 No Hit CAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAA 9 0.22499999999999998 No Hit CCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTT 9 0.22499999999999998 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 9 0.22499999999999998 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 9 0.22499999999999998 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 9 0.22499999999999998 No Hit TAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGA 9 0.22499999999999998 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 9 0.22499999999999998 No Hit GCAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAA 8 0.2 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 8 0.2 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 7 0.17500000000000002 No Hit GCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTG 7 0.17500000000000002 No Hit GCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCA 7 0.17500000000000002 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 7 0.17500000000000002 No Hit CCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAA 7 0.17500000000000002 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 7 0.17500000000000002 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 7 0.17500000000000002 No Hit CACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCA 7 0.17500000000000002 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 7 0.17500000000000002 No Hit CACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCG 7 0.17500000000000002 No Hit TCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTT 7 0.17500000000000002 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 6 0.15 No Hit GCCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 6 0.15 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 6 0.15 No Hit ATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTT 6 0.15 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 6 0.15 No Hit GGGTGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 6 0.15 No Hit ATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAG 6 0.15 No Hit TAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAA 6 0.15 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 6 0.15 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 6 0.15 No Hit AGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTAT 6 0.15 No Hit CTTCCATACCAAGATTAGCACGGTTGATGATATCAGCCCAAGTATTAATA 6 0.15 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 5 0.125 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 5 0.125 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 5 0.125 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 5 0.125 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 5 0.125 No Hit CATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAAG 5 0.125 No Hit CTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAGCTGCAAC 5 0.125 No Hit GATTGGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACC 5 0.125 No Hit GTCCACAGTATCAAATAGATCCTTTTCTTCTTTAGGAATTCTACCAAGGG 5 0.125 No Hit AGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAAGCAG 5 0.125 No Hit TCCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 5 0.125 No Hit GGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGC 5 0.125 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 5 0.125 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 5 0.125 No Hit GCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAGCTGCAA 5 0.125 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 5 0.125 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 5 0.125 No Hit ACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAG 5 0.125 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0125 0.0 0.0 0.0 0.0 92-93 0.025 0.0 0.0 0.0 0.0 94-95 0.025 0.0 0.0 0.0 0.0 96-97 0.025 0.0 0.0 0.0 0.0 98-99 0.025 0.0 0.0 0.0 0.0 100-101 0.025 0.0 0.0 0.0 0.0 102-103 0.05 0.0 0.0 0.0 0.0 104-105 0.05 0.0 0.0 0.0 0.0 106-107 0.05 0.0 0.0 0.0 0.0 108-109 0.05 0.0 0.0 0.0 0.0 110-111 0.05 0.0 0.0 0.0 0.0 112-113 0.075 0.0 0.0 0.0 0.0 114-115 0.075 0.0 0.0 0.0 0.0 116-117 0.075 0.0 0.0 0.0 0.0 118-119 0.1 0.0 0.0 0.0 0.0 120-121 0.125 0.0 0.0 0.0 0.0 122-123 0.175 0.0 0.0 0.0 0.0 124-125 0.2 0.0 0.0 0.0 0.0 126-127 0.2 0.0 0.0 0.0 0.0 128-129 0.21250000000000002 0.0 0.0 0.0 0.0 130-131 0.25 0.0 0.0 0.0 0.0 132-133 0.275 0.0 0.0 0.0 0.0 134-135 0.3125 0.0 0.0 0.0 0.0 136-137 0.3375 0.0 0.0 0.0 0.0 138 0.4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTTGT 10 0.006973645 144.0 9 CGCTGGA 10 0.006973645 144.0 8 TTCGCTG 10 0.006973645 144.0 6 TGCTTTT 10 0.006973645 144.0 7 TCGCTGG 10 0.006973645 144.0 7 GGTGGTT 10 0.006973645 144.0 1 GTGGTTC 10 0.006973645 144.0 2 GCTTTTG 10 0.006973645 144.0 8 GTTGCTT 10 0.006973645 144.0 5 TTGCTTT 10 0.006973645 144.0 6 GTTCGCT 10 0.006973645 144.0 5 CTTTCTT 25 8.956223E-4 86.399994 2 GCTTTCT 30 0.0018473949 72.0 1 CTTTTCT 35 0.0034045284 61.714283 6 TTTCTTC 35 0.0034045284 61.714283 8 TCTTTTC 35 0.0034045284 61.714283 5 TTCTTCA 40 0.005777437 54.0 9 TTTCTTT 45 0.009205684 48.0 3 TTTTCTT 45 0.009205684 48.0 7 TTCTTTT 45 0.009205684 48.0 4 >>END_MODULE ERR11006592 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006592_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.61525 37.0 37.0 37.0 37.0 37.0 2 36.0735 37.0 37.0 37.0 37.0 37.0 3 36.2155 37.0 37.0 37.0 37.0 37.0 4 36.2065 37.0 37.0 37.0 37.0 37.0 5 36.243 37.0 37.0 37.0 37.0 37.0 6 36.2095 37.0 37.0 37.0 37.0 37.0 7 36.303 37.0 37.0 37.0 37.0 37.0 8 36.362 37.0 37.0 37.0 37.0 37.0 9 36.285 37.0 37.0 37.0 37.0 37.0 10-14 36.2047 37.0 37.0 37.0 37.0 37.0 15-19 36.1983 37.0 37.0 37.0 37.0 37.0 20-24 36.1774 37.0 37.0 37.0 37.0 37.0 25-29 36.123900000000006 37.0 37.0 37.0 37.0 37.0 30-34 36.0852 37.0 37.0 37.0 37.0 37.0 35-39 36.076499999999996 37.0 37.0 37.0 37.0 37.0 40-44 36.06699999999999 37.0 37.0 37.0 37.0 37.0 45-49 36.1072 37.0 37.0 37.0 37.0 37.0 50-54 36.0654 37.0 37.0 37.0 37.0 37.0 55-59 36.024800000000006 37.0 37.0 37.0 37.0 37.0 60-64 35.907999999999994 37.0 37.0 37.0 37.0 37.0 65-69 35.92739999999999 37.0 37.0 37.0 37.0 37.0 70-74 35.9176 37.0 37.0 37.0 37.0 37.0 75-79 35.919900000000005 37.0 37.0 37.0 37.0 37.0 80-84 35.8361 37.0 37.0 37.0 37.0 37.0 85-89 35.7758 37.0 37.0 37.0 37.0 37.0 90-94 35.737700000000004 37.0 37.0 37.0 37.0 37.0 95-99 35.610400000000006 37.0 37.0 37.0 37.0 37.0 100-104 35.6468 37.0 37.0 37.0 37.0 37.0 105-109 35.528200000000005 37.0 37.0 37.0 37.0 37.0 110-114 35.499700000000004 37.0 37.0 37.0 37.0 37.0 115-119 35.3907 37.0 37.0 37.0 34.6 37.0 120-124 35.3227 37.0 37.0 37.0 32.2 37.0 125-129 35.3812 37.0 37.0 37.0 37.0 37.0 130-134 35.320100000000004 37.0 37.0 37.0 32.2 37.0 135-139 35.2084 37.0 37.0 37.0 25.0 37.0 140-144 35.090900000000005 37.0 37.0 37.0 25.0 37.0 145-149 35.1437 37.0 37.0 37.0 27.4 37.0 150 34.849 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 0.0 14 0.0 15 0.0 16 0.0 17 1.0 18 0.0 19 0.0 20 2.0 21 0.0 22 3.0 23 10.0 24 3.0 25 8.0 26 11.0 27 16.0 28 20.0 29 39.0 30 45.0 31 55.0 32 81.0 33 116.0 34 207.0 35 552.0 36 2655.0 37 175.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.931454683929932 24.346280781924346 16.78090886011678 28.94135567402894 2 28.075 25.25 29.175 17.5 3 23.3 27.950000000000003 28.749999999999996 20.0 4 23.200000000000003 28.375 26.174999999999997 22.25 5 25.7 31.374999999999996 24.25 18.675 6 22.0 32.875 25.825 19.3 7 19.575 21.475 38.224999999999994 20.724999999999998 8 23.3 23.425 29.65 23.625 9 23.825 21.9 32.4 21.875 10-14 24.18 26.765 27.3 21.755 15-19 24.395 25.115 29.154999999999998 21.335 20-24 25.19 24.565 28.95 21.295 25-29 23.93 25.014999999999997 29.425 21.63 30-34 25.085 24.295 29.615000000000002 21.005 35-39 25.7 24.41 28.49 21.4 40-44 24.02 24.915000000000003 29.244999999999997 21.82 45-49 24.395 25.88 28.23 21.495 50-54 24.635 25.230000000000004 28.43 21.705 55-59 23.955000000000002 25.25 27.665 23.13 60-64 23.175 25.130000000000003 29.020000000000003 22.675 65-69 24.9 24.535 28.525 22.040000000000003 70-74 25.05 25.135 28.375 21.44 75-79 24.759999999999998 24.745 29.275000000000002 21.22 80-84 25.195 25.185000000000002 28.63 20.990000000000002 85-89 24.73 24.585 27.975 22.71 90-94 24.055 24.515 28.544999999999998 22.884999999999998 95-99 23.075000000000003 25.3 29.825000000000003 21.8 100-104 23.655 25.31 29.53 21.505 105-109 24.975 24.48 29.330000000000002 21.215 110-114 25.635 24.585 28.29 21.490000000000002 115-119 23.69 25.369999999999997 28.58 22.36 120-124 24.245 25.115 28.425 22.215 125-129 23.09 25.480000000000004 29.185 22.245 130-134 23.875 25.505 28.63 21.990000000000002 135-139 23.78 24.86 29.509999999999998 21.85 140-144 24.005000000000003 25.314999999999998 28.915000000000003 21.765 145-149 23.595 24.765 28.794999999999998 22.845 150 22.7 25.674999999999997 30.025000000000002 21.6 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 1.0 4 0.5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 1.5 21 2.0 22 2.0 23 4.0 24 8.0 25 11.5 26 16.5 27 19.0 28 17.5 29 24.5 30 38.0 31 38.0 32 40.5 33 45.0 34 49.0 35 80.0 36 116.0 37 163.0 38 196.5 39 183.5 40 183.0 41 205.5 42 211.5 43 218.5 44 220.5 45 212.5 46 201.0 47 149.5 48 112.0 49 96.5 50 74.5 51 63.5 52 48.0 53 37.0 54 32.5 55 35.5 56 39.5 57 35.5 58 33.0 59 38.0 60 45.5 61 40.5 62 37.0 63 62.0 64 83.0 65 82.5 66 59.0 67 33.5 68 33.0 69 25.5 70 22.0 71 27.5 72 28.0 73 25.0 74 22.5 75 18.0 76 10.0 77 9.0 78 9.0 79 6.0 80 4.5 81 3.5 82 1.5 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.525 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.55 #Duplication Level Percentage of deduplicated Percentage of total 1 80.77188146106134 58.599999999999994 2 12.405237767057201 18.0 3 2.963473466574776 6.45 4 1.3783597518952446 4.0 5 0.9648518263266712 3.5000000000000004 6 0.44796691936595456 1.95 7 0.44796691936595456 2.275 8 0.10337698139214334 0.6 9 0.10337698139214334 0.675 >10 0.41350792556857335 3.95 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 19 0.475 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 18 0.44999999999999996 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 15 0.375 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 14 0.35000000000000003 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 13 0.325 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 12 0.3 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 12 0.3 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 12 0.3 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 12 0.3 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 11 0.27499999999999997 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 10 0.25 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 10 0.25 No Hit ATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCAC 9 0.22499999999999998 No Hit GAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCG 9 0.22499999999999998 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 9 0.22499999999999998 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 8 0.2 No Hit CTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTTATGA 8 0.2 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 8 0.2 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 7 0.17500000000000002 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 7 0.17500000000000002 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 7 0.17500000000000002 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 7 0.17500000000000002 No Hit TGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCAT 7 0.17500000000000002 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 7 0.17500000000000002 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 7 0.17500000000000002 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 7 0.17500000000000002 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 7 0.17500000000000002 No Hit ATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATT 7 0.17500000000000002 No Hit CTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTAC 7 0.17500000000000002 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 7 0.17500000000000002 No Hit TGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACT 7 0.17500000000000002 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 6 0.15 No Hit TGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATAC 6 0.15 No Hit ATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGC 6 0.15 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 6 0.15 No Hit AATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTAT 6 0.15 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 6 0.15 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 6 0.15 No Hit TAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAG 6 0.15 No Hit GCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGC 6 0.15 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 6 0.15 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 6 0.15 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 6 0.15 No Hit GAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGT 6 0.15 No Hit TGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAAT 5 0.125 No Hit CCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTAT 5 0.125 No Hit GGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTC 5 0.125 No Hit CTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCG 5 0.125 No Hit CCGCAACTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGAT 5 0.125 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 5 0.125 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 5 0.125 No Hit CCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGG 5 0.125 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 5 0.125 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 5 0.125 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 5 0.125 No Hit TTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGA 5 0.125 No Hit GGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGGCCTGGTCT 5 0.125 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 5 0.125 No Hit CGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCC 5 0.125 No Hit CTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTT 5 0.125 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 5 0.125 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 5 0.125 No Hit CTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAG 5 0.125 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 5 0.125 No Hit TATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTAT 5 0.125 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 5 0.125 No Hit GCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAG 5 0.125 No Hit GTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGA 5 0.125 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 5 0.125 No Hit AACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGG 5 0.125 No Hit GTGGACGTTGCCGTAGCGCTGCGGGCCTGGTCTGGGTGTGCTACTGATGG 5 0.125 No Hit AGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0125 0.0 0.0 0.0 0.0 92-93 0.025 0.0 0.0 0.0 0.0 94-95 0.025 0.0 0.0 0.0 0.0 96-97 0.025 0.0 0.0 0.0 0.0 98-99 0.025 0.0 0.0 0.0 0.0 100-101 0.025 0.0 0.0 0.0 0.0 102-103 0.05 0.0 0.0 0.0 0.0 104-105 0.05 0.0 0.0 0.0 0.0 106-107 0.05 0.0 0.0 0.0 0.0 108-109 0.05 0.0 0.0 0.0 0.0 110-111 0.05 0.0 0.0 0.0 0.0 112-113 0.075 0.0 0.0 0.0 0.0 114-115 0.075 0.0 0.0 0.0 0.0 116-117 0.075 0.0 0.0 0.0 0.0 118-119 0.1 0.0 0.0 0.0 0.0 120-121 0.125 0.0 0.0 0.0 0.0 122-123 0.175 0.0 0.0 0.0 0.0 124-125 0.2 0.0 0.0 0.0 0.0 126-127 0.2 0.0 0.0 0.0 0.0 128-129 0.21250000000000002 0.0 0.0 0.0 0.0 130-131 0.25 0.0 0.0 0.0 0.0 132-133 0.275 0.0 0.0 0.0 0.0 134-135 0.3125 0.0 0.0 0.0 0.0125 136-137 0.3625 0.0 0.0 0.0 0.025 138 0.425 0.0 0.0 0.0 0.025 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCAGG 10 0.0067147487 145.81013 1 GGGTCTG 10 0.0069754543 143.9875 6 AGGGTCT 10 0.0069754543 143.9875 5 ACATCGT 10 0.0069754543 143.9875 6 CCAGGGT 10 0.0069754543 143.9875 3 AACATCG 10 0.0069754543 143.9875 5 >>END_MODULE Read 2569760 spots for ERR11006592.sra Written 2569760 spots for ERR11006592.sra Read 2569760 spots for ERR11006592.sra Written 2569760 spots for ERR11006592.sra Read 2569760 spots for ERR11006592.sra Written 2569760 spots for ERR11006592.sra Read 2569760 spots for ERR11006592.sra Written 2569760 spots for ERR11006592.sra Read 2569760 spots for ERR11006592.sra Written 2569760 spots for ERR11006592.sra Read 2569760 spots for ERR11006592.sra Written 2569760 spots for ERR11006592.sra Read 2569760 spots for ERR11006592.sra Written 2569760 spots for ERR11006592.sra Read 2569760 spots for ERR11006592.sra Written 2569760 spots for ERR11006592.sra Read 2569760 spots for ERR11006592.sra Written 2569760 spots for ERR11006592.sra Read 2569760 spots for ERR11006592.sra Written 2569760 spots for ERR11006592.sra Read 2569760 spots for ERR11006592.sra Written 2569760 spots for ERR11006592.sra Read 2569760 spots for ERR11006592.sra Written 2569760 spots for ERR11006592.sra Read 2569760 spots for ERR11006592.sra Written 2569760 spots for ERR11006592.sra Read 2569760 spots for ERR11006592.sra Written 2569760 spots for ERR11006592.sra Read 2569760 spots for ERR11006592.sra Written 2569760 spots for ERR11006592.sra Read 2569760 spots for ERR11006592.sra Written 2569760 spots for ERR11006592.sra Read 2569773 spots for ERR11006592.sra Written 2569773 spots for ERR11006592.sra Read 2569760 spots for ERR11006592.sra Written 2569760 spots for ERR11006592.sra Read 2569760 spots for ERR11006592.sra Written 2569760 spots for ERR11006592.sra Read 2569760 spots for ERR11006592.sra Written 2569760 spots for ERR11006592.sra SRR ids: ['ERR11006592.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_16m7l9aq ERR11006592.sra spots: 51395213 blocks: [[1, 2569760], [2569761, 5139520], [5139521, 7709280], [7709281, 10279040], [10279041, 12848800], [12848801, 15418560], [15418561, 17988320], [17988321, 20558080], [20558081, 23127840], [23127841, 25697600], [25697601, 28267360], [28267361, 30837120], [30837121, 33406880], [33406881, 35976640], [35976641, 38546400], [38546401, 41116160], [41116161, 43685920], [43685921, 46255680], [46255681, 48825440], [48825441, 51395213]] ERR11006592 file size 18884217 ERR11006592 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006592 ERR11006592_1.fastq ERR11006592_2.fastq Input file: ERR11006592_1.fastq Paired file: ERR11006592_2.fastq trimmed: ERR11006592-trimmed-pair1.fastq, ERR11006592-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 18:48:32 2024 >> started Fri Dec 6 18:49:32 2024 >> done (60.428s) 51395213 read pairs processed; of these: 197 ( 0.00%) short read pairs filtered out after trimming by size control 2689 ( 0.01%) empty read pairs filtered out after trimming by size control 51392327 (99.99%) read pairs available; of these: 505927 ( 0.98%) trimmed read pairs available after processing 50886400 (99.02%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 10 0.00% 19 9 0.00% 20 20 0.00% 21 10 0.00% 22 23 0.00% 23 19 0.00% 24 10 0.00% 25 14 0.00% 26 11 0.00% 27 18 0.00% 28 15 0.00% 29 18 0.00% 30 15 0.00% 31 87 0.00% 32 18 0.00% 33 12 0.00% 34 11 0.00% 35 20 0.00% 36 13 0.00% 37 21 0.00% 38 16 0.00% 39 23 0.00% 40 22 0.00% 41 24 0.00% 42 24 0.00% 43 19 0.00% 44 23 0.00% 45 26 0.00% 46 32 0.00% 47 38 0.00% 48 40 0.00% 49 31 0.00% 50 43 0.00% 51 67 0.00% 52 67 0.00% 53 74 0.00% 54 68 0.00% 55 66 0.00% 56 69 0.00% 57 97 0.00% 58 97 0.00% 59 103 0.00% 60 138 0.00% 61 135 0.00% 62 152 0.00% 63 167 0.00% 64 157 0.00% 65 220 0.00% 66 204 0.00% 67 199 0.00% 68 244 0.00% 69 265 0.00% 70 283 0.00% 71 301 0.00% 72 367 0.00% 73 405 0.00% 74 453 0.00% 75 427 0.00% 76 508 0.00% 77 510 0.00% 78 591 0.00% 79 564 0.00% 80 617 0.00% 81 656 0.00% 82 658 0.00% 83 782 0.00% 84 866 0.00% 85 869 0.00% 86 1024 0.00% 87 1172 0.00% 88 1132 0.00% 89 1229 0.00% 90 1324 0.00% 91 1385 0.00% 92 1457 0.00% 93 1511 0.00% 94 1584 0.00% 95 1645 0.00% 96 1774 0.00% 97 1975 0.00% 98 2060 0.00% 99 2055 0.00% 100 2279 0.00% 101 2379 0.00% 102 2366 0.00% 103 2546 0.00% 104 2665 0.01% 105 2780 0.01% 106 3102 0.01% 107 3267 0.01% 108 3380 0.01% 109 3616 0.01% 110 3888 0.01% 111 3995 0.01% 112 4201 0.01% 113 4241 0.01% 114 4511 0.01% 115 4858 0.01% 116 5356 0.01% 117 5729 0.01% 118 5631 0.01% 119 6019 0.01% 120 6333 0.01% 121 6876 0.01% 122 7036 0.01% 123 7469 0.01% 124 7713 0.02% 125 8570 0.02% 126 8362 0.02% 127 8253 0.02% 128 8809 0.02% 129 9434 0.02% 130 10153 0.02% 131 10865 0.02% 132 11363 0.02% 133 11333 0.02% 134 11518 0.02% 135 12062 0.02% 136 12564 0.02% 137 13348 0.03% 138 14017 0.03% 139 14585 0.03% 140 15504 0.03% 141 15936 0.03% 142 17139 0.03% 143 18169 0.04% 144 18447 0.04% 145 19447 0.04% 146 21201 0.04% 147 21771 0.04% 148 23031 0.04% 149 24302 0.05% 150 50886400 99.02% 51392327 reads passed initial QC criterion=sequence-density sequence-density=0.33 sequence-density-rank=1 fanout-score=1.00 fanout-score-rank=38 prefix-density=0.01 prefix-fanout=1.0 sequence=GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTTATCCTTCCACCGTTGGAAGCGGGCAGTTGTCGCTGCTCTGTGAAGCCAGCCTCACGCTGTGCCTGCCAACATTATGGGCCGCGAAGCCTAGCTTTCGCTTAAGCTCCAACGGCCCACTACGCAACTTGGAACGGGCGGGCCATCAGTAGCACACCCAGACCAGGCCCGCAGCGCTACGGCAACGTCCACACCACCCTTAAAGCCCCCACT criterion=fanout-score sequence-density=0.02 sequence-density-rank=36 fanout-score=50.58 fanout-score-rank=1 prefix-density=0.18 prefix-fanout=5.1 sequence=ATAAAAAAAAGGGGGGGTAAGGACCCGCTAAGCTCCTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGATGAACCCAATCCAGAATATGAACCATAAAAGAAAACACCTACTAAACCAATCACAAGAATACCAGTTACCGTACCTATCAGCCAAAGAGGAATTCTTCCAGTAGTATCGGCCATTTCCCCTACTTTCCTCCACATTTTATCAAGTGGTCATGCTAGAGACAAAAACAGTCATGGATAGTTATGTTATAAGGATGGTATCCTTCCAAATGGGATAAGAGAGTTCTTACTACTCTCTTCTTTTCTCTCAATT criterion=sequence-density sequence-density=0.30 sequence-density-rank=1 fanout-score=12.17 fanout-score-rank=6 prefix-density=3.65 prefix-fanout=1.0 sequence=TTGCGTAGTGGATCTGCTGGGGCCTATGCGAAAGCTGGGCCTCACGAATTCTATAGTGGCAGGCACCGCGTTAGGCTGGCTTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=94.46 fanout-score-rank=1 prefix-density=0.08 prefix-fanout=10.7 sequence=TTTTTTTTTATGAGATTTTTGCTAAAGTTTCATTTACGCCTAATTCACATCGAGTAGACCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAACTAAAGCAAGTGTTGGATTTAAAGCTGGTGTTAAAGATTATAGATTGACTTACTACACCCCGGAGTATGAAACCAAGGATACTGATATCTTGGCAGCATTCCGAGTATCTCCTCAACCTGGGGTTCCGCCCGAAGAAGCAGGGGCTGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTTTGGACTGATGGACTTACTAGTCTTGATCGTTACAAAGGACGATGCTATCACATCGAGCCTGTTCCTGGGGAAGACAGTCAATGGATCTGTTATGTAGCTTATCCATTAGATCTATTTGAAGAGGGTTCCGTTACTAACATGTTTACTTCCATTGTAGGTAACGTATTTGGTTTCAAAGCCCTACGTGCTCTACGTCTG ERR11006592 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 18:51:00 Started mapping on | Dec 06 18:51:01 Finished on | Dec 06 18:56:20 Mapping speed, Million of reads per hour | 579.98 Number of input reads | 51392327 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 38422713 Uniquely mapped reads % | 74.76% Average mapped length | 298.32 Number of splices: Total | 14901886 Number of splices: Annotated (sjdb) | 13966388 Number of splices: GT/AG | 14589763 Number of splices: GC/AG | 165439 Number of splices: AT/AC | 27920 Number of splices: Non-canonical | 118764 Mismatch rate per base, % | 0.30% Deletion rate per base | 0.02% Deletion average length | 1.64 Insertion rate per base | 0.01% Insertion average length | 2.00 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 11280337 % of reads mapped to multiple loci | 21.95% Number of reads mapped to too many loci | 13877 % of reads mapped to too many loci | 0.03% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.73% % of reads unmapped: other | 0.53% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1689277 1689277 1689277 N_multimapping 11280337 11280337 11280337 N_noFeature 9609941 34804883 12280755 N_ambiguous 1571561 44955 620076 UnstrandedReadsAssigned:27241211 PositiveStrandReadsAssigned:3572875 NegativeStrandReadsAssigned:25521882 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006592 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006592-trimmed-pair1.fastq ERR11006592-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 51,392,327 reads, 29,959,251 reads pseudoaligned [quant] estimated average fragment length: 259.598 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,106 rounds 52973 ERR11006592.ke.tsv 35125 ERR11006592.se.tsv 88098 total ==> ERR11006592.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 677.754 0 0 PNS24247 1044 785.402 14.0572 0.594574 PNS24249 1928 1669.4 129.106 2.56913 PNS24246 1044 785.402 14.0572 0.594574 PNS24248 1044 785.402 14.0572 0.594574 PNS24244 1471 1212.4 63.7225 1.74601 PNS24243 293 55.5475 0 0 KQK14069 1603 1344.4 485.559 11.9981 KQK14071 474 217.122 15.3503 2.34862 ==> ERR11006592.se.tsv <== BRADI_1g14170v3 575 BRADI_1g53295v3 2222 BRADI_1g59795v3 388 BRADI_1g07683v3 0 BRADI_1g00485v3 6 BRADI_1g20270v3 129 BRADI_1g74790v3 245 BRADI_1g09890v3 3 BRADI_1g77505v3 69 BRADI_1g48960v3 0 ERR11006592 completed mapping pipeline successfully