Starting /dee2/code/volunteer_pipeline.sh ERR11006593 current disk space = 1550198333440 free memory = 1600030484 ERR11006593 SRAfilesize 663549d9aba0a68e24e3e3bf90095e0a ERR11006593.sra ERR11006593.sra file validated ERR11006593 is paired end ERR11006593 is conventional basespace ERR11006593 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006593_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.3315 37.0 37.0 37.0 37.0 37.0 2 36.191 37.0 37.0 37.0 37.0 37.0 3 36.3075 37.0 37.0 37.0 37.0 37.0 4 36.421 37.0 37.0 37.0 37.0 37.0 5 36.4275 37.0 37.0 37.0 37.0 37.0 6 36.474 37.0 37.0 37.0 37.0 37.0 7 36.3405 37.0 37.0 37.0 37.0 37.0 8 36.362 37.0 37.0 37.0 37.0 37.0 9 36.478 37.0 37.0 37.0 37.0 37.0 10-14 36.409800000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.4322 37.0 37.0 37.0 37.0 37.0 20-24 36.3815 37.0 37.0 37.0 37.0 37.0 25-29 36.37089999999999 37.0 37.0 37.0 37.0 37.0 30-34 36.2783 37.0 37.0 37.0 37.0 37.0 35-39 36.263200000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.1934 37.0 37.0 37.0 37.0 37.0 45-49 36.152 37.0 37.0 37.0 37.0 37.0 50-54 36.1364 37.0 37.0 37.0 37.0 37.0 55-59 36.1915 37.0 37.0 37.0 37.0 37.0 60-64 36.0988 37.0 37.0 37.0 37.0 37.0 65-69 36.107000000000006 37.0 37.0 37.0 37.0 37.0 70-74 36.134100000000004 37.0 37.0 37.0 37.0 37.0 75-79 36.0255 37.0 37.0 37.0 37.0 37.0 80-84 35.995099999999994 37.0 37.0 37.0 37.0 37.0 85-89 35.953700000000005 37.0 37.0 37.0 37.0 37.0 90-94 35.9637 37.0 37.0 37.0 37.0 37.0 95-99 35.897299999999994 37.0 37.0 37.0 37.0 37.0 100-104 35.8493 37.0 37.0 37.0 37.0 37.0 105-109 35.7768 37.0 37.0 37.0 37.0 37.0 110-114 35.7813 37.0 37.0 37.0 37.0 37.0 115-119 35.7975 37.0 37.0 37.0 37.0 37.0 120-124 35.562599999999996 37.0 37.0 37.0 37.0 37.0 125-129 35.5424 37.0 37.0 37.0 37.0 37.0 130-134 35.4701 37.0 37.0 37.0 37.0 37.0 135-139 35.411100000000005 37.0 37.0 37.0 37.0 37.0 140-144 35.3197 37.0 37.0 37.0 34.6 37.0 145-149 35.2796 37.0 37.0 37.0 34.6 37.0 150 35.3105 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 0.0 21 0.0 22 1.0 23 4.0 24 2.0 25 6.0 26 6.0 27 13.0 28 18.0 29 28.0 30 37.0 31 56.0 32 93.0 33 106.0 34 188.0 35 376.0 36 2807.0 37 258.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.075 14.625 15.0 34.300000000000004 2 29.25 13.750000000000002 27.400000000000002 29.599999999999998 3 25.074999999999996 16.375 25.775 32.775 4 27.200000000000003 18.35 23.474999999999998 30.975 5 28.075 22.650000000000002 24.099999999999998 25.174999999999997 6 26.375 30.375000000000004 21.05 22.2 7 15.1 31.674999999999997 34.449999999999996 18.775 8 16.024036054081122 29.24386579869805 33.50025037556335 21.231847771657485 9 17.625 27.575 33.625 21.175 10-14 19.919999999999998 32.46 26.584999999999997 21.035 15-19 20.935000000000002 32.08 25.5 21.485000000000003 20-24 19.869999999999997 30.385 26.865 22.88 25-29 22.055 30.95 25.71 21.285 30-34 22.685 30.490000000000002 25.419999999999998 21.404999999999998 35-39 21.305 31.235000000000003 25.94 21.52 40-44 20.085 30.975 26.634999999999998 22.305 45-49 20.605 30.819999999999997 25.990000000000002 22.585 50-54 21.395 31.275 25.840000000000003 21.490000000000002 55-59 21.17 30.37 24.735 23.724999999999998 60-64 20.45 31.474999999999998 25.96 22.115000000000002 65-69 21.085 30.459999999999997 25.185000000000002 23.27 70-74 22.03 30.470000000000002 24.42 23.080000000000002 75-79 21.9 30.64 25.629999999999995 21.83 80-84 22.035 29.64 25.36 22.965 85-89 21.959999999999997 29.87 25.75 22.42 90-94 21.09 30.070000000000004 26.36 22.48 95-99 21.615000000000002 29.709999999999997 25.86 22.814999999999998 100-104 21.08 31.005 24.740000000000002 23.175 105-109 20.605 29.45 26.72 23.225 110-114 21.33 29.445 26.07 23.155 115-119 20.945 31.155 24.959999999999997 22.939999999999998 120-124 20.119999999999997 29.439999999999998 26.1 24.34 125-129 21.005 30.080000000000002 24.740000000000002 24.175 130-134 21.605 30.665 25.040000000000003 22.689999999999998 135-139 21.925 29.29 24.990000000000002 23.794999999999998 140-144 22.1 29.525000000000002 26.72 21.654999999999998 145-149 21.675 30.98 25.665 21.68 150 22.125 29.549999999999997 26.025 22.3 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 1.0 3 0.5 4 0.5 5 1.0 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0 20 2.0 21 1.0 22 0.0 23 2.0 24 5.0 25 6.0 26 11.0 27 16.0 28 18.5 29 25.0 30 31.0 31 36.5 32 45.5 33 55.0 34 59.5 35 64.0 36 103.5 37 181.5 38 253.5 39 239.0 40 219.5 41 275.5 42 275.0 43 236.0 44 220.5 45 215.5 46 200.0 47 156.5 48 126.0 49 110.5 50 82.5 51 64.5 52 53.5 53 38.0 54 36.0 55 36.0 56 30.0 57 28.0 58 26.5 59 28.0 60 29.0 61 27.0 62 28.0 63 26.5 64 37.0 65 41.5 66 30.5 67 20.5 68 18.5 69 17.0 70 14.0 71 13.5 72 12.0 73 15.0 74 15.0 75 10.5 76 6.0 77 4.5 78 6.0 79 4.5 80 1.5 81 1.0 82 1.0 83 0.5 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.15 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.65 #Duplication Level Percentage of deduplicated Percentage of total 1 84.79571332886805 63.3 2 9.84594775619558 14.7 3 1.9758874748827864 4.425 4 0.971198928332217 2.9000000000000004 5 0.7367716008037508 2.75 6 0.5023442732752846 2.25 7 0.4353650368385801 2.275 8 0.16744809109176156 1.0 9 0.23442732752846618 1.575 >10 0.33489618218352313 4.825 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 48 1.2 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 26 0.65 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 25 0.625 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 16 0.4 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 16 0.4 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 15 0.375 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 13 0.325 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 12 0.3 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 12 0.3 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 10 0.25 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 9 0.22499999999999998 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 9 0.22499999999999998 No Hit CAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAA 9 0.22499999999999998 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 9 0.22499999999999998 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 9 0.22499999999999998 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 9 0.22499999999999998 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 9 0.22499999999999998 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 8 0.2 No Hit GCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCA 8 0.2 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 8 0.2 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 8 0.2 No Hit CAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTG 8 0.2 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 7 0.17500000000000002 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 7 0.17500000000000002 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 7 0.17500000000000002 No Hit AGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAG 7 0.17500000000000002 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 7 0.17500000000000002 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 7 0.17500000000000002 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 7 0.17500000000000002 No Hit CCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTA 7 0.17500000000000002 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 7 0.17500000000000002 No Hit AGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTAT 7 0.17500000000000002 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 7 0.17500000000000002 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 7 0.17500000000000002 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 7 0.17500000000000002 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 6 0.15 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 6 0.15 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 6 0.15 No Hit CTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTG 6 0.15 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 6 0.15 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 6 0.15 No Hit AATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTT 6 0.15 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 6 0.15 No Hit GTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCA 6 0.15 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 6 0.15 No Hit GCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAAGCT 6 0.15 No Hit CAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGA 6 0.15 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 6 0.15 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 6 0.15 No Hit TAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGA 6 0.15 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 5 0.125 No Hit GGGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACA 5 0.125 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 5 0.125 No Hit GCCGTTTTTCAATGGTCTCGAAGATATAAATTTTTTCATTTTTATCTATG 5 0.125 No Hit AGGGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAAC 5 0.125 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 5 0.125 No Hit GCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTAAG 5 0.125 No Hit CATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAAG 5 0.125 No Hit CCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTT 5 0.125 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 5 0.125 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 5 0.125 No Hit GCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTG 5 0.125 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 5 0.125 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 5 0.125 No Hit AACGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGG 5 0.125 No Hit TAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCA 5 0.125 No Hit GGCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGAT 5 0.125 No Hit GAGCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTA 5 0.125 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 5 0.125 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 5 0.125 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 5 0.125 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0125 16-17 0.0 0.0 0.0 0.0 0.025 18-19 0.0 0.0 0.0 0.0 0.025 20-21 0.025 0.0 0.0 0.0 0.025 22-23 0.025 0.0 0.0 0.0 0.025 24-25 0.025 0.0 0.0 0.0 0.025 26-27 0.025 0.0 0.0 0.0 0.025 28-29 0.025 0.0 0.0 0.0 0.025 30-31 0.025 0.0 0.0 0.0 0.025 32-33 0.025 0.0 0.0 0.0 0.025 34-35 0.025 0.0 0.0 0.0 0.025 36-37 0.025 0.0 0.0 0.0 0.025 38-39 0.025 0.0 0.0 0.0 0.025 40-41 0.025 0.0 0.0 0.0 0.025 42-43 0.025 0.0 0.0 0.0 0.025 44-45 0.025 0.0 0.0 0.0 0.025 46-47 0.025 0.0 0.0 0.0 0.025 48-49 0.025 0.0 0.0 0.0 0.025 50-51 0.025 0.0 0.0 0.0 0.025 52-53 0.025 0.0 0.0 0.0 0.025 54-55 0.025 0.0 0.0 0.0 0.025 56-57 0.025 0.0 0.0 0.0 0.025 58-59 0.025 0.0 0.0 0.0 0.025 60-61 0.025 0.0 0.0 0.0 0.025 62-63 0.025 0.0 0.0 0.0 0.025 64-65 0.025 0.0 0.0 0.0 0.025 66-67 0.025 0.0 0.0 0.0 0.025 68-69 0.025 0.0 0.0 0.0 0.025 70-71 0.05 0.0 0.0 0.0 0.025 72-73 0.05 0.0 0.0 0.0 0.025 74-75 0.07500000000000001 0.0 0.0 0.0 0.025 76-77 0.1375 0.0 0.0 0.0 0.025 78-79 0.16249999999999998 0.0 0.0 0.0 0.025 80-81 0.175 0.0 0.0 0.0 0.025 82-83 0.175 0.0 0.0 0.0 0.025 84-85 0.175 0.0 0.0 0.0 0.025 86-87 0.175 0.0 0.0 0.0 0.025 88-89 0.21250000000000002 0.0 0.0 0.0 0.025 90-91 0.25 0.0 0.0 0.0 0.025 92-93 0.3 0.0 0.0 0.0 0.025 94-95 0.3375 0.0 0.0 0.0 0.025 96-97 0.4 0.0 0.0 0.0 0.025 98-99 0.425 0.0 0.0 0.0 0.025 100-101 0.4625 0.0 0.0 0.0 0.025 102-103 0.5 0.0 0.0 0.0 0.025 104-105 0.5625 0.0 0.0 0.0 0.025 106-107 0.6875 0.0 0.0 0.0 0.025 108-109 0.825 0.0 0.0 0.0 0.025 110-111 0.9125000000000001 0.0 0.0 0.0 0.025 112-113 1.0125 0.0 0.0 0.0 0.025 114-115 1.1 0.0 0.0 0.0 0.025 116-117 1.2374999999999998 0.0 0.0 0.0 0.025 118-119 1.4 0.0 0.0 0.0 0.025 120-121 1.525 0.0 0.0 0.0 0.025 122-123 1.6875 0.0 0.0 0.0 0.025 124-125 1.775 0.0 0.0 0.0 0.025 126-127 1.8375 0.0 0.0 0.0 0.025 128-129 1.925 0.0 0.0 0.0 0.025 130-131 2.0875 0.0 0.0 0.0 0.025 132-133 2.3499999999999996 0.0 0.0 0.0 0.025 134-135 2.5375 0.0 0.0 0.0 0.025 136-137 2.6624999999999996 0.0 0.0 0.0 0.025 138 2.8 0.0 0.0 0.0 0.025 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAAATTT 10 0.006973645 144.0 9 >>END_MODULE ERR11006593 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006593_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.9265 37.0 37.0 37.0 37.0 37.0 2 35.987 37.0 37.0 37.0 37.0 37.0 3 36.1525 37.0 37.0 37.0 37.0 37.0 4 36.0795 37.0 37.0 37.0 37.0 37.0 5 36.2425 37.0 37.0 37.0 37.0 37.0 6 36.052 37.0 37.0 37.0 37.0 37.0 7 36.254 37.0 37.0 37.0 37.0 37.0 8 36.2145 37.0 37.0 37.0 37.0 37.0 9 36.1615 37.0 37.0 37.0 37.0 37.0 10-14 36.1442 37.0 37.0 37.0 37.0 37.0 15-19 36.166700000000006 37.0 37.0 37.0 37.0 37.0 20-24 36.1778 37.0 37.0 37.0 37.0 37.0 25-29 36.1361 37.0 37.0 37.0 37.0 37.0 30-34 36.078100000000006 37.0 37.0 37.0 37.0 37.0 35-39 36.0837 37.0 37.0 37.0 37.0 37.0 40-44 36.0037 37.0 37.0 37.0 37.0 37.0 45-49 35.954899999999995 37.0 37.0 37.0 37.0 37.0 50-54 35.918400000000005 37.0 37.0 37.0 37.0 37.0 55-59 35.8634 37.0 37.0 37.0 37.0 37.0 60-64 35.795100000000005 37.0 37.0 37.0 37.0 37.0 65-69 35.765 37.0 37.0 37.0 37.0 37.0 70-74 35.61 37.0 37.0 37.0 37.0 37.0 75-79 35.7418 37.0 37.0 37.0 37.0 37.0 80-84 35.6882 37.0 37.0 37.0 37.0 37.0 85-89 35.6405 37.0 37.0 37.0 37.0 37.0 90-94 35.62 37.0 37.0 37.0 37.0 37.0 95-99 35.4779 37.0 37.0 37.0 37.0 37.0 100-104 35.5599 37.0 37.0 37.0 37.0 37.0 105-109 35.363 37.0 37.0 37.0 29.8 37.0 110-114 35.3456 37.0 37.0 37.0 34.6 37.0 115-119 35.3041 37.0 37.0 37.0 29.8 37.0 120-124 35.134499999999996 37.0 37.0 37.0 27.4 37.0 125-129 35.0712 37.0 37.0 37.0 25.0 37.0 130-134 34.9298 37.0 37.0 37.0 25.0 37.0 135-139 34.8515 37.0 37.0 37.0 25.0 37.0 140-144 34.8377 37.0 37.0 37.0 25.0 37.0 145-149 34.68150000000001 37.0 37.0 37.0 25.0 37.0 150 34.6545 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 0.0 21 2.0 22 1.0 23 3.0 24 5.0 25 7.0 26 8.0 27 17.0 28 20.0 29 38.0 30 60.0 31 53.0 32 92.0 33 164.0 34 275.0 35 732.0 36 2408.0 37 114.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.325000000000003 27.35 16.125 27.200000000000003 2 24.474999999999998 28.675 32.225 14.625 3 21.4 30.2 30.75 17.65 4 23.275000000000002 30.675 26.375 19.675 5 23.0 32.525 27.224999999999998 17.25 6 19.950000000000003 35.6 27.474999999999998 16.975 7 19.5 21.9 38.975 19.625 8 20.65 25.650000000000002 30.275000000000002 23.425 9 22.575 21.45 34.275 21.7 10-14 23.419999999999998 27.41 27.875 21.295 15-19 23.630000000000003 26.365 29.42 20.585 20-24 23.855 25.619999999999997 29.23 21.295 25-29 23.13 26.235000000000003 29.69 20.945 30-34 22.759999999999998 26.0 30.435000000000002 20.805 35-39 24.04 25.405 29.630000000000003 20.925 40-44 23.22 26.195 29.445 21.14 45-49 23.59 27.315 28.32 20.775 50-54 23.195 26.185000000000002 30.175 20.445 55-59 23.25 26.200000000000003 29.335 21.215 60-64 22.955000000000002 25.95 29.880000000000003 21.215 65-69 23.275000000000002 25.885 29.29 21.55 70-74 23.165 25.569999999999997 30.049999999999997 21.215 75-79 23.98 25.4 29.555 21.065 80-84 24.01 24.959999999999997 30.365 20.665 85-89 23.36 26.634999999999998 29.07 20.935000000000002 90-94 23.125 26.040000000000003 29.255 21.58 95-99 23.385 25.564999999999998 30.020000000000003 21.029999999999998 100-104 23.580000000000002 26.029999999999998 29.885 20.505000000000003 105-109 23.189999999999998 25.840000000000003 29.48 21.490000000000002 110-114 23.36 25.569999999999997 30.225 20.845 115-119 23.13 26.779999999999998 29.609999999999996 20.48 120-124 23.61 26.135 28.74 21.515 125-129 23.07 25.45 30.064999999999998 21.415 130-134 23.44 25.840000000000003 29.775000000000002 20.945 135-139 23.635 26.135 30.275000000000002 19.955000000000002 140-144 23.755000000000003 26.38 29.21 20.655 145-149 23.294999999999998 25.900000000000002 29.54 21.265 150 23.599999999999998 25.324999999999996 30.049999999999997 21.025 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.5 18 0.5 19 0.0 20 2.0 21 3.5 22 2.5 23 3.5 24 4.5 25 11.0 26 17.5 27 20.5 28 25.5 29 30.5 30 39.5 31 44.0 32 49.5 33 55.5 34 62.5 35 78.5 36 104.0 37 162.5 38 212.5 39 212.0 40 230.0 41 246.5 42 222.5 43 228.0 44 266.0 45 244.0 46 196.0 47 160.5 48 125.0 49 98.5 50 69.0 51 57.0 52 50.5 53 44.5 54 38.5 55 30.5 56 29.0 57 33.5 58 37.0 59 37.5 60 37.5 61 35.5 62 37.0 63 37.0 64 35.5 65 37.0 66 24.5 67 14.5 68 22.0 69 24.5 70 20.0 71 16.5 72 13.0 73 10.0 74 12.0 75 11.5 76 6.0 77 3.5 78 1.5 79 1.0 80 3.0 81 3.0 82 1.5 83 1.5 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.97500000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 83.8762750904903 63.725 2 10.661401776900297 16.2 3 2.3692003948667324 5.4 4 0.9213557091148403 2.8000000000000003 5 0.6910167818361304 2.625 6 0.42777229351760443 1.95 7 0.2632444883185258 1.4000000000000001 8 0.2632444883185258 1.6 9 0.19743336623889435 1.35 >10 0.3290556103981573 2.9499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 14 0.35000000000000003 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 14 0.35000000000000003 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 12 0.3 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 12 0.3 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 12 0.3 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 11 0.27499999999999997 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 11 0.27499999999999997 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 11 0.27499999999999997 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 11 0.27499999999999997 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 10 0.25 No Hit ATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCAC 9 0.22499999999999998 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 9 0.22499999999999998 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 9 0.22499999999999998 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 9 0.22499999999999998 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 9 0.22499999999999998 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 9 0.22499999999999998 No Hit CTATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGA 8 0.2 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 8 0.2 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 8 0.2 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 8 0.2 No Hit AATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTAT 8 0.2 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 8 0.2 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 8 0.2 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 8 0.2 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 7 0.17500000000000002 No Hit GTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACT 7 0.17500000000000002 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 7 0.17500000000000002 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 7 0.17500000000000002 No Hit GCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAG 7 0.17500000000000002 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 7 0.17500000000000002 No Hit GTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAAT 7 0.17500000000000002 No Hit ATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAA 7 0.17500000000000002 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 6 0.15 No Hit ATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGG 6 0.15 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 6 0.15 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 6 0.15 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 6 0.15 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 6 0.15 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 6 0.15 No Hit CTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGC 6 0.15 No Hit AATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGT 6 0.15 No Hit CTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAG 6 0.15 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 6 0.15 No Hit CATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGGCG 6 0.15 No Hit TGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACT 6 0.15 No Hit CTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGT 5 0.125 No Hit TTCACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCT 5 0.125 No Hit ATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCA 5 0.125 No Hit TGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCAT 5 0.125 No Hit TGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATA 5 0.125 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 5 0.125 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 5 0.125 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 5 0.125 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 5 0.125 No Hit GGACAACTGTTTGGACTGATGGACTTACTAGTCTTGATCGTTACAAAGGA 5 0.125 No Hit AGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAG 5 0.125 No Hit TCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTA 5 0.125 No Hit AAGGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAAC 5 0.125 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 5 0.125 No Hit TTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGG 5 0.125 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 5 0.125 No Hit AGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACT 5 0.125 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 5 0.125 No Hit AACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGG 5 0.125 No Hit GAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTT 5 0.125 No Hit AGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.07500000000000001 0.0 0.0 0.0 0.0 76-77 0.1375 0.0 0.0 0.0 0.0 78-79 0.16249999999999998 0.0 0.0 0.0 0.0 80-81 0.175 0.0 0.0 0.0 0.0 82-83 0.175 0.0 0.0 0.0 0.0 84-85 0.175 0.0 0.0 0.0 0.0 86-87 0.175 0.0 0.0 0.0 0.0 88-89 0.21250000000000002 0.0 0.0 0.0 0.0 90-91 0.25 0.0 0.0 0.0 0.0 92-93 0.325 0.0 0.0 0.0 0.0 94-95 0.3625 0.0 0.0 0.0 0.0 96-97 0.425 0.0 0.0 0.0 0.0 98-99 0.4625 0.0 0.0 0.0 0.0 100-101 0.5125 0.0 0.0 0.0 0.0 102-103 0.55 0.0 0.0 0.0 0.0 104-105 0.625 0.0 0.0 0.0 0.0 106-107 0.7625 0.0 0.0 0.0 0.0 108-109 0.8999999999999999 0.0 0.0 0.0 0.0 110-111 0.9875 0.0 0.0 0.0 0.0 112-113 1.0625 0.0 0.0 0.0 0.0 114-115 1.15 0.0 0.0 0.0 0.0 116-117 1.2875 0.0 0.0 0.0 0.0 118-119 1.45 0.0 0.0 0.0 0.0 120-121 1.5875 0.0 0.0 0.0 0.0 122-123 1.7625000000000002 0.0 0.0 0.0 0.0 124-125 1.85 0.0 0.0 0.0 0.0 126-127 1.9375 0.0 0.0 0.0 0.0 128-129 2.0250000000000004 0.0 0.0 0.0 0.0 130-131 2.2 0.0 0.0 0.0 0.0 132-133 2.45 0.0 0.0 0.0 0.0 134-135 2.6625 0.0 0.0 0.0 0.0 136-137 2.8 0.0 0.0 0.0 0.0 138 2.95 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGTGT 10 0.006973645 144.0 7 ATGTGTG 10 0.006973645 144.0 8 ACTTCGT 10 0.006973645 144.0 5 TGTGTGC 10 0.006973645 144.0 9 GTTAATA 10 0.006973645 144.0 2 AGTTAAT 15 1.1730364E-4 144.0 1 >>END_MODULE Read 2209812 spots for ERR11006593.sra Written 2209812 spots for ERR11006593.sra Read 2209812 spots for ERR11006593.sra Written 2209812 spots for ERR11006593.sra Read 2209812 spots for ERR11006593.sra Written 2209812 spots for ERR11006593.sra Read 2209812 spots for ERR11006593.sra Written 2209812 spots for ERR11006593.sra Read 2209812 spots for ERR11006593.sra Written 2209812 spots for ERR11006593.sra Read 2209812 spots for ERR11006593.sra Written 2209812 spots for ERR11006593.sra Read 2209812 spots for ERR11006593.sra Written 2209812 spots for ERR11006593.sra Read 2209812 spots for ERR11006593.sra Written 2209812 spots for ERR11006593.sra Read 2209812 spots for ERR11006593.sra Written 2209812 spots for ERR11006593.sra Read 2209812 spots for ERR11006593.sra Written 2209812 spots for ERR11006593.sra Read 2209812 spots for ERR11006593.sra Written 2209812 spots for ERR11006593.sra Read 2209812 spots for ERR11006593.sra Written 2209812 spots for ERR11006593.sra Read 2209812 spots for ERR11006593.sra Written 2209812 spots for ERR11006593.sra Read 2209812 spots for ERR11006593.sra Written 2209812 spots for ERR11006593.sra Read 2209831 spots for ERR11006593.sra Written 2209831 spots for ERR11006593.sra Read 2209812 spots for ERR11006593.sra Written 2209812 spots for ERR11006593.sra Read 2209812 spots for ERR11006593.sra Written 2209812 spots for ERR11006593.sra Read 2209812 spots for ERR11006593.sra Written 2209812 spots for ERR11006593.sra Read 2209812 spots for ERR11006593.sra Written 2209812 spots for ERR11006593.sra Read 2209812 spots for ERR11006593.sra Written 2209812 spots for ERR11006593.sra SRR ids: ['ERR11006593.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_hp30dnlz ERR11006593.sra spots: 44196259 blocks: [[1, 2209812], [2209813, 4419624], [4419625, 6629436], [6629437, 8839248], [8839249, 11049060], [11049061, 13258872], [13258873, 15468684], [15468685, 17678496], [17678497, 19888308], [19888309, 22098120], [22098121, 24307932], [24307933, 26517744], [26517745, 28727556], [28727557, 30937368], [30937369, 33147180], [33147181, 35356992], [35356993, 37566804], [37566805, 39776616], [39776617, 41986428], [41986429, 44196259]] ERR11006593 file size 16237593 ERR11006593 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006593 ERR11006593_1.fastq ERR11006593_2.fastq Input file: ERR11006593_1.fastq Paired file: ERR11006593_2.fastq trimmed: ERR11006593-trimmed-pair1.fastq, ERR11006593-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 18:49:18 2024 >> started Fri Dec 6 18:50:33 2024 >> done (74.349s) 44196259 read pairs processed; of these: 497 ( 0.00%) short read pairs filtered out after trimming by size control 4950 ( 0.01%) empty read pairs filtered out after trimming by size control 44190812 (99.99%) read pairs available; of these: 2403206 ( 5.44%) trimmed read pairs available after processing 41787606 (94.56%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 15 0.00% 19 14 0.00% 20 28 0.00% 21 44 0.00% 22 29 0.00% 23 26 0.00% 24 41 0.00% 25 4033 0.01% 26 46 0.00% 27 27 0.00% 28 24 0.00% 29 36 0.00% 30 30 0.00% 31 109 0.00% 32 33 0.00% 33 26 0.00% 34 24 0.00% 35 26 0.00% 36 46 0.00% 37 30 0.00% 38 36 0.00% 39 28 0.00% 40 37 0.00% 41 46 0.00% 42 55 0.00% 43 57 0.00% 44 61 0.00% 45 73 0.00% 46 65 0.00% 47 84 0.00% 48 88 0.00% 49 99 0.00% 50 91 0.00% 51 130 0.00% 52 123 0.00% 53 154 0.00% 54 177 0.00% 55 464 0.00% 56 221 0.00% 57 256 0.00% 58 300 0.00% 59 329 0.00% 60 355 0.00% 61 419 0.00% 62 529 0.00% 63 550 0.00% 64 670 0.00% 65 700 0.00% 66 807 0.00% 67 819 0.00% 68 943 0.00% 69 961 0.00% 70 1099 0.00% 71 1206 0.00% 72 1390 0.00% 73 1590 0.00% 74 1915 0.00% 75 2065 0.00% 76 2335 0.01% 77 2459 0.01% 78 2724 0.01% 79 2847 0.01% 80 3097 0.01% 81 3405 0.01% 82 3716 0.01% 83 4099 0.01% 84 4464 0.01% 85 5291 0.01% 86 5847 0.01% 87 6420 0.01% 88 6749 0.02% 89 7086 0.02% 90 7415 0.02% 91 7699 0.02% 92 8226 0.02% 93 8972 0.02% 94 9302 0.02% 95 10274 0.02% 96 11074 0.03% 97 11727 0.03% 98 12562 0.03% 99 12844 0.03% 100 14009 0.03% 101 14111 0.03% 102 14795 0.03% 103 15578 0.04% 104 16699 0.04% 105 17857 0.04% 106 19357 0.04% 107 19433 0.04% 108 20373 0.05% 109 21535 0.05% 110 22598 0.05% 111 23506 0.05% 112 24990 0.06% 113 24505 0.06% 114 26087 0.06% 115 27514 0.06% 116 30396 0.07% 117 31068 0.07% 118 31639 0.07% 119 33542 0.08% 120 35787 0.08% 121 37036 0.08% 122 38782 0.09% 123 39289 0.09% 124 41480 0.09% 125 43155 0.10% 126 43067 0.10% 127 43041 0.10% 128 45660 0.10% 129 47475 0.11% 130 50895 0.12% 131 53536 0.12% 132 54063 0.12% 133 52741 0.12% 134 54515 0.12% 135 55347 0.13% 136 57105 0.13% 137 59158 0.13% 138 61868 0.14% 139 65674 0.15% 140 67440 0.15% 141 70852 0.16% 142 73990 0.17% 143 74493 0.17% 144 75238 0.17% 145 78704 0.18% 146 84383 0.19% 147 84694 0.19% 148 89252 0.20% 149 90581 0.20% 150 41787606 94.56% 44190812 reads passed initial QC criterion=sequence-density sequence-density=0.24 sequence-density-rank=1 fanout-score=3.66 fanout-score-rank=23 prefix-density=0.30 prefix-fanout=2.9 sequence=AAAAAACAACGT criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=68.51 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=1.8 sequence=AAAAGTATTAATACAGTCACATGGATGGTAAATGCGTGAATGTGATGGACTAAAAAATCTGCAGTTCCTAATGGAATAGGTAACAAAGCCACTTTGCCGCCTACTGCTACTAACTCGCCACCTCCCCACGTTAAACTGGTACTTGTTGTTGCACCAGGAGCTGTTACACCAGGCGCGTTAGCATGGATATTTTGTACCCATTGAGCAAAGATAGGTTGTAATTGTATGGCAGTATCCGAAAACATATCTTGCGGACGTCCTAAAGCACTCATGGTATCATTATGAATGTACAAACCAAAACTGTGAAAACCTAGAAATATACATACCCAGTTAAGGTGGGATATGATTGCATCACGGTGTCTAAGGACGCGATCTAATAGATCATTGTATCGAGTAGTTGGATCATAGTCTCTTACCATAAAAATTGCTGCATGTGCAGCAGCACCGACTATTAGAAATCCGCCAATCCACATGTGGTGTGTGAACAAGGAAAGTTGTGTACCATAGTCAG criterion=sequence-density sequence-density=0.36 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=35 prefix-density=0.37 prefix-fanout=2.0 sequence=GCGGCTCTCCTA criterion=fanout-score sequence-density=0.02 sequence-density-rank=37 fanout-score=58.45 fanout-score-rank=1 prefix-density=0.12 prefix-fanout=9.9 sequence=TTTTTTTTTATGAGATTTTTGCTAAAGTTTCATTTACGCCTAATTCACATCGAGTAGACCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAACTAAAGCAAGTGTTGGATTTAAAGCTGGTGTTAAAGATTATAGATTGACTTACTACACCCCGGAGTATGAAACCAAGGATACTGATATCTTGGCAGCATTCCGAGTATCTCCTCAACCTGGGGTTCCGCCCGAAGAAGCAGGGGCTGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTTTGGACTGATGGACTTACTAGTCTTGATCGTTACAAAGGACGATGCTATCACATCGAGCCTGTTCCTGGGGAAGACAGTCAATGGATCTGTTATGTAGCTTATCCATTAGATCTATTTGAAGAGGGTTCCGTTACTAACATGTTTACTTCCATTGTAGGTAACGTATTTGGTTTCAAAGCCCTACGTGCTCTACGTCTG ERR11006593 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 18:51:10 Started mapping on | Dec 06 18:51:10 Finished on | Dec 06 18:56:36 Mapping speed, Million of reads per hour | 488.00 Number of input reads | 44190812 Average input read length | 297 UNIQUE READS: Uniquely mapped reads number | 30391268 Uniquely mapped reads % | 68.77% Average mapped length | 296.66 Number of splices: Total | 12696814 Number of splices: Annotated (sjdb) | 11925437 Number of splices: GT/AG | 12417579 Number of splices: GC/AG | 148535 Number of splices: AT/AC | 27110 Number of splices: Non-canonical | 103590 Mismatch rate per base, % | 0.31% Deletion rate per base | 0.02% Deletion average length | 1.72 Insertion rate per base | 0.01% Insertion average length | 1.96 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 12169640 % of reads mapped to multiple loci | 27.54% Number of reads mapped to too many loci | 6707 % of reads mapped to too many loci | 0.02% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.38% % of reads unmapped: other | 0.29% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1629904 1629904 1629904 N_multimapping 12169640 12169640 12169640 N_noFeature 8549076 28192776 10072731 N_ambiguous 1231769 35113 557339 UnstrandedReadsAssigned:20610423 PositiveStrandReadsAssigned:2163379 NegativeStrandReadsAssigned:19761198 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006593 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006593-trimmed-pair1.fastq ERR11006593-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 44,190,812 reads, 27,553,412 reads pseudoaligned [quant] estimated average fragment length: 225.618 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,298 rounds 52973 ERR11006593.ke.tsv 35125 ERR11006593.se.tsv 88098 total ==> ERR11006593.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 711.69 0 0 PNS24247 1044 819.382 21.1847 1.01049 PNS24249 1928 1703.38 106.861 2.45189 PNS24246 1044 819.382 21.1847 1.01049 PNS24248 1044 819.382 21.1847 1.01049 PNS24244 1471 1246.38 52.585 1.64894 PNS24243 293 78.4354 0 0 KQK14069 1603 1378.38 756.739 21.4571 KQK14071 474 250.749 115.716 18.0363 ==> ERR11006593.se.tsv <== BRADI_1g14170v3 961 BRADI_1g53295v3 995 BRADI_1g59795v3 332 BRADI_1g07683v3 0 BRADI_1g00485v3 3 BRADI_1g20270v3 222 BRADI_1g74790v3 190 BRADI_1g09890v3 0 BRADI_1g77505v3 130 BRADI_1g48960v3 0 ERR11006593 completed mapping pipeline successfully