Starting /dee2/code/volunteer_pipeline.sh ERR11006594 current disk space = 1550199119872 free memory = 1599724684 ERR11006594 SRAfilesize f51d2e5a93d1433cc05587c8823d4dfb ERR11006594.sra ERR11006594.sra file validated ERR11006594 is paired end ERR11006594 is conventional basespace ERR11006594 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006594_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.50825 37.0 37.0 37.0 37.0 37.0 2 36.4285 37.0 37.0 37.0 37.0 37.0 3 36.483 37.0 37.0 37.0 37.0 37.0 4 36.58 37.0 37.0 37.0 37.0 37.0 5 36.555 37.0 37.0 37.0 37.0 37.0 6 36.5375 37.0 37.0 37.0 37.0 37.0 7 36.492 37.0 37.0 37.0 37.0 37.0 8 36.449 37.0 37.0 37.0 37.0 37.0 9 36.495 37.0 37.0 37.0 37.0 37.0 10-14 36.443 37.0 37.0 37.0 37.0 37.0 15-19 36.521499999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.46 37.0 37.0 37.0 37.0 37.0 25-29 36.394 37.0 37.0 37.0 37.0 37.0 30-34 36.400999999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.3184 37.0 37.0 37.0 37.0 37.0 40-44 36.3617 37.0 37.0 37.0 37.0 37.0 45-49 36.2794 37.0 37.0 37.0 37.0 37.0 50-54 36.2487 37.0 37.0 37.0 37.0 37.0 55-59 36.2458 37.0 37.0 37.0 37.0 37.0 60-64 36.2543 37.0 37.0 37.0 37.0 37.0 65-69 36.1811 37.0 37.0 37.0 37.0 37.0 70-74 36.07299999999999 37.0 37.0 37.0 37.0 37.0 75-79 36.08540000000001 37.0 37.0 37.0 37.0 37.0 80-84 36.1113 37.0 37.0 37.0 37.0 37.0 85-89 35.968500000000006 37.0 37.0 37.0 37.0 37.0 90-94 35.9741 37.0 37.0 37.0 37.0 37.0 95-99 35.9185 37.0 37.0 37.0 37.0 37.0 100-104 35.991 37.0 37.0 37.0 37.0 37.0 105-109 35.8366 37.0 37.0 37.0 37.0 37.0 110-114 35.84140000000001 37.0 37.0 37.0 37.0 37.0 115-119 35.7944 37.0 37.0 37.0 37.0 37.0 120-124 35.6841 37.0 37.0 37.0 37.0 37.0 125-129 35.6272 37.0 37.0 37.0 37.0 37.0 130-134 35.670700000000004 37.0 37.0 37.0 37.0 37.0 135-139 35.5213 37.0 37.0 37.0 37.0 37.0 140-144 35.390299999999996 37.0 37.0 37.0 34.6 37.0 145-149 35.474900000000005 37.0 37.0 37.0 34.6 37.0 150 35.084 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 0.0 18 0.0 19 1.0 20 0.0 21 1.0 22 2.0 23 2.0 24 1.0 25 5.0 26 8.0 27 11.0 28 23.0 29 19.0 30 40.0 31 47.0 32 64.0 33 97.0 34 142.0 35 355.0 36 2912.0 37 269.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.40910227556889 14.553638409602401 13.87846961740435 35.15878969742436 2 28.625 13.625000000000002 28.525 29.225 3 25.35 16.275000000000002 25.6 32.775 4 28.9 18.25 23.075000000000003 29.775000000000002 5 29.625 23.7 21.825 24.85 6 26.125 31.35 19.650000000000002 22.875 7 16.150000000000002 30.325000000000003 34.475 19.05 8 16.425 29.175 33.025 21.375 9 18.65 27.625 32.175 21.55 10-14 20.32 31.355 26.14 22.185 15-19 21.224999999999998 31.130000000000003 24.935 22.71 20-24 20.77 29.465000000000003 25.96 23.805 25-29 21.475 30.314999999999998 26.05 22.16 30-34 22.835 30.205 24.875 22.085 35-39 22.16 29.880000000000003 25.385 22.575 40-44 21.17 29.17 26.015 23.645 45-49 21.060000000000002 29.69 26.08 23.169999999999998 50-54 21.19 31.785000000000004 24.79 22.235 55-59 22.509999999999998 28.465 24.755 24.27 60-64 20.565 29.9 25.724999999999998 23.810000000000002 65-69 21.505 30.665 24.86 22.97 70-74 21.884999999999998 29.880000000000003 23.825 24.41 75-79 22.384999999999998 29.525000000000002 25.569999999999997 22.52 80-84 21.9 29.18 25.06 23.86 85-89 22.415 29.78 24.505 23.3 90-94 21.55 29.825000000000003 25.330000000000002 23.294999999999998 95-99 22.345000000000002 29.535 24.52 23.599999999999998 100-104 21.525 30.095 24.815 23.565 105-109 22.009999999999998 29.18 25.285000000000004 23.525 110-114 21.375 29.39 24.69 24.545 115-119 20.919999999999998 30.919999999999998 24.79 23.369999999999997 120-124 21.099999999999998 29.759999999999998 24.695 24.445 125-129 21.395 29.549999999999997 23.995 25.06 130-134 22.015 29.765000000000004 24.25 23.97 135-139 23.064999999999998 28.99 23.775 24.169999999999998 140-144 23.125 29.970000000000002 25.040000000000003 21.865000000000002 145-149 22.495 30.54 24.305 22.66 150 23.25 28.875 24.925 22.95 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.0 4 1.5 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 0.5 20 0.5 21 1.0 22 3.5 23 5.5 24 5.5 25 5.5 26 6.5 27 10.0 28 15.0 29 15.0 30 21.5 31 31.5 32 30.5 33 32.5 34 44.5 35 59.5 36 89.0 37 173.0 38 236.5 39 231.0 40 237.0 41 286.0 42 274.0 43 213.0 44 197.5 45 199.0 46 192.5 47 172.5 48 138.5 49 104.0 50 84.0 51 64.5 52 57.0 53 49.0 54 46.5 55 44.0 56 32.0 57 33.0 58 38.5 59 39.5 60 34.0 61 25.5 62 19.5 63 21.0 64 36.0 65 54.0 66 42.5 67 24.5 68 22.0 69 22.0 70 23.0 71 23.5 72 23.0 73 19.5 74 18.0 75 16.0 76 10.0 77 10.0 78 11.0 79 5.5 80 7.5 81 7.0 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.1 #Duplication Level Percentage of deduplicated Percentage of total 1 83.60323886639675 61.95 2 10.323886639676113 15.299999999999999 3 2.8677462887989202 6.375 4 0.9109311740890688 2.7 5 0.5735492577597842 2.125 6 0.6747638326585695 3.0 7 0.20242914979757085 1.05 8 0.2699055330634278 1.6 9 0.20242914979757085 1.35 >10 0.33738191632928477 3.25 >50 0.033738191632928474 1.3 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 52 1.3 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 21 0.525 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 16 0.4 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 13 0.325 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 13 0.325 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 13 0.325 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 12 0.3 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 11 0.27499999999999997 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 11 0.27499999999999997 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 10 0.25 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 10 0.25 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 9 0.22499999999999998 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 9 0.22499999999999998 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 9 0.22499999999999998 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 9 0.22499999999999998 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 9 0.22499999999999998 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 9 0.22499999999999998 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 8 0.2 No Hit GAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAAC 8 0.2 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 8 0.2 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 8 0.2 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 8 0.2 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 8 0.2 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 8 0.2 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 8 0.2 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 7 0.17500000000000002 No Hit GCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGT 7 0.17500000000000002 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 7 0.17500000000000002 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 7 0.17500000000000002 No Hit AGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATAT 7 0.17500000000000002 No Hit GGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGG 7 0.17500000000000002 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 6 0.15 No Hit CCGCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGAT 6 0.15 No Hit AGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAG 6 0.15 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 6 0.15 No Hit CAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAA 6 0.15 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 6 0.15 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 6 0.15 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 6 0.15 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 6 0.15 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 6 0.15 No Hit GTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAG 6 0.15 No Hit TCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGT 6 0.15 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 6 0.15 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 6 0.15 No Hit CACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCA 6 0.15 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 6 0.15 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 6 0.15 No Hit ACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACT 6 0.15 No Hit TAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGA 6 0.15 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 6 0.15 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 5 0.125 No Hit ACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGA 5 0.125 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 5 0.125 No Hit GCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCA 5 0.125 No Hit GGGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGC 5 0.125 No Hit CCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAG 5 0.125 No Hit GATTGGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACC 5 0.125 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 5 0.125 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 5 0.125 No Hit AGGAGCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAAC 5 0.125 No Hit CGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAAC 5 0.125 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 5 0.125 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 5 0.125 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 5 0.125 No Hit AGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTAT 5 0.125 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 5 0.125 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.037500000000000006 0.0 0.0 0.0 0.0 88-89 0.05 0.0 0.0 0.0 0.0 90-91 0.05 0.0 0.0 0.0 0.0 92-93 0.075 0.0 0.0 0.0 0.0 94-95 0.075 0.0 0.0 0.0 0.0 96-97 0.1 0.0 0.0 0.0 0.0 98-99 0.16249999999999998 0.0 0.0 0.0 0.0 100-101 0.1875 0.0 0.0 0.0 0.0 102-103 0.25 0.0 0.0 0.0 0.0 104-105 0.3 0.0 0.0 0.0 0.0 106-107 0.36250000000000004 0.0 0.0 0.0 0.0 108-109 0.5 0.0 0.0 0.0 0.0 110-111 0.5874999999999999 0.0 0.0 0.0 0.0 112-113 0.6625 0.0 0.0 0.0 0.0 114-115 0.8125 0.0 0.0 0.0 0.0 116-117 0.85 0.0 0.0 0.0 0.0 118-119 0.925 0.0 0.0 0.0 0.0 120-121 1.125 0.0 0.0 0.0 0.0 122-123 1.1875 0.0 0.0 0.0 0.0 124-125 1.275 0.0 0.0 0.0 0.0 126-127 1.4 0.0 0.0 0.0 0.0 128-129 1.5 0.0 0.0 0.0 0.0 130-131 1.625 0.0 0.0 0.0 0.0 132-133 1.7875 0.0 0.0 0.0 0.0 134-135 2.0 0.0 0.0 0.0 0.0 136-137 2.1 0.0 0.0 0.0 0.0 138 2.1 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCGTAA 10 0.006973645 144.0 3 AACCAGT 10 0.006973645 144.0 8 TAACCAG 10 0.006973645 144.0 7 CGTAACC 15 1.1730364E-4 144.0 5 TCGTAAC 10 0.006973645 144.0 4 >>END_MODULE ERR11006594 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006594_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.107 37.0 37.0 37.0 37.0 37.0 2 36.2655 37.0 37.0 37.0 37.0 37.0 3 36.3335 37.0 37.0 37.0 37.0 37.0 4 36.3 37.0 37.0 37.0 37.0 37.0 5 36.374 37.0 37.0 37.0 37.0 37.0 6 36.377 37.0 37.0 37.0 37.0 37.0 7 36.4 37.0 37.0 37.0 37.0 37.0 8 36.4445 37.0 37.0 37.0 37.0 37.0 9 36.3845 37.0 37.0 37.0 37.0 37.0 10-14 36.3982 37.0 37.0 37.0 37.0 37.0 15-19 36.347300000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.3468 37.0 37.0 37.0 37.0 37.0 25-29 36.23909999999999 37.0 37.0 37.0 37.0 37.0 30-34 36.2502 37.0 37.0 37.0 37.0 37.0 35-39 36.182199999999995 37.0 37.0 37.0 37.0 37.0 40-44 36.231399999999994 37.0 37.0 37.0 37.0 37.0 45-49 36.2013 37.0 37.0 37.0 37.0 37.0 50-54 36.0837 37.0 37.0 37.0 37.0 37.0 55-59 36.0517 37.0 37.0 37.0 37.0 37.0 60-64 36.0554 37.0 37.0 37.0 37.0 37.0 65-69 35.9396 37.0 37.0 37.0 37.0 37.0 70-74 35.9286 37.0 37.0 37.0 37.0 37.0 75-79 35.8451 37.0 37.0 37.0 37.0 37.0 80-84 35.8602 37.0 37.0 37.0 37.0 37.0 85-89 35.874399999999994 37.0 37.0 37.0 37.0 37.0 90-94 35.8508 37.0 37.0 37.0 37.0 37.0 95-99 35.7611 37.0 37.0 37.0 37.0 37.0 100-104 35.6597 37.0 37.0 37.0 37.0 37.0 105-109 35.6101 37.0 37.0 37.0 37.0 37.0 110-114 35.5863 37.0 37.0 37.0 37.0 37.0 115-119 35.400099999999995 37.0 37.0 37.0 34.6 37.0 120-124 35.4477 37.0 37.0 37.0 34.6 37.0 125-129 35.376400000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.2313 37.0 37.0 37.0 29.8 37.0 135-139 35.2073 37.0 37.0 37.0 25.0 37.0 140-144 35.093900000000005 37.0 37.0 37.0 25.0 37.0 145-149 34.837599999999995 37.0 37.0 37.0 25.0 37.0 150 34.9385 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 1.0 17 0.0 18 0.0 19 0.0 20 1.0 21 0.0 22 2.0 23 2.0 24 3.0 25 8.0 26 5.0 27 11.0 28 13.0 29 23.0 30 36.0 31 54.0 32 79.0 33 126.0 34 213.0 35 679.0 36 2555.0 37 188.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.299999999999997 25.174999999999997 18.425 27.1 2 26.200000000000003 27.275 31.775 14.75 3 21.099999999999998 28.299999999999997 31.35 19.25 4 23.200000000000003 29.349999999999998 25.95 21.5 5 24.5 32.4 26.075 17.025000000000002 6 20.849999999999998 34.55 25.825 18.775 7 19.625 22.55 39.550000000000004 18.275 8 23.5 23.5 29.299999999999997 23.7 9 24.775 20.724999999999998 31.3 23.200000000000003 10-14 23.724999999999998 26.69 28.13 21.455 15-19 24.035 25.465 29.215000000000003 21.285 20-24 24.495 25.115 29.325000000000003 21.065 25-29 23.45 25.130000000000003 29.659999999999997 21.759999999999998 30-34 24.275 25.040000000000003 29.82 20.865000000000002 35-39 24.59 25.380000000000003 28.74 21.29 40-44 24.08 25.235000000000003 29.830000000000002 20.855 45-49 23.330000000000002 26.525 28.985 21.16 50-54 24.565 25.055 28.910000000000004 21.47 55-59 23.365 25.22 28.599999999999998 22.814999999999998 60-64 23.06 25.119999999999997 29.544999999999998 22.275 65-69 24.005000000000003 25.255 29.285 21.455 70-74 24.404999999999998 25.085 29.025000000000002 21.485000000000003 75-79 24.595 25.28 29.09 21.035 80-84 24.135 24.715 29.544999999999998 21.605 85-89 23.775 24.72 29.299999999999997 22.205 90-94 22.865 24.515 29.709999999999997 22.91 95-99 23.74 24.73 28.88 22.650000000000002 100-104 23.380000000000003 24.474999999999998 30.73 21.415 105-109 25.074999999999996 24.275 28.634999999999998 22.015 110-114 23.335 25.145 29.549999999999997 21.97 115-119 24.305 25.419999999999998 28.87 21.404999999999998 120-124 24.04 25.11 28.810000000000002 22.040000000000003 125-129 24.4 24.675 29.24 21.685 130-134 23.73 25.4 29.015 21.855 135-139 24.035 24.7 30.154999999999998 21.11 140-144 23.78 25.22 29.599999999999998 21.4 145-149 23.98 25.15 29.26 21.61 150 24.3 24.325 30.15 21.224999999999998 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 2.0 23 3.0 24 2.5 25 9.0 26 15.0 27 13.0 28 19.0 29 28.5 30 31.5 31 37.0 32 53.5 33 61.0 34 61.0 35 66.0 36 103.0 37 177.0 38 195.5 39 202.5 40 233.5 41 225.5 42 198.0 43 207.5 44 227.0 45 217.0 46 207.5 47 166.0 48 121.5 49 99.5 50 86.0 51 76.5 52 58.0 53 46.5 54 41.5 55 41.0 56 34.5 57 29.5 58 37.5 59 35.0 60 31.0 61 32.5 62 31.5 63 41.5 64 48.0 65 45.5 66 38.0 67 36.5 68 39.0 69 34.0 70 27.0 71 19.5 72 20.5 73 23.0 74 16.5 75 9.0 76 7.5 77 8.0 78 6.0 79 3.5 80 2.5 81 3.0 82 2.0 83 1.0 84 1.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.8 #Duplication Level Percentage of deduplicated Percentage of total 1 83.0548128342246 62.125 2 10.929144385026738 16.35 3 2.6737967914438503 6.0 4 1.2700534759358288 3.8 5 0.7018716577540107 2.625 6 0.2339572192513369 1.05 7 0.4344919786096257 2.275 8 0.16711229946524064 1.0 9 0.16711229946524064 1.125 >10 0.3676470588235294 3.65 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 20 0.5 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 15 0.375 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 15 0.375 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 14 0.35000000000000003 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 14 0.35000000000000003 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 13 0.325 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 12 0.3 No Hit TTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGA 11 0.27499999999999997 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 11 0.27499999999999997 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 11 0.27499999999999997 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 10 0.25 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 9 0.22499999999999998 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 9 0.22499999999999998 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 9 0.22499999999999998 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 9 0.22499999999999998 No Hit CAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTC 9 0.22499999999999998 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 8 0.2 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 8 0.2 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 8 0.2 No Hit TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC 8 0.2 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 8 0.2 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 7 0.17500000000000002 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 7 0.17500000000000002 No Hit CTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATT 7 0.17500000000000002 No Hit ACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTC 7 0.17500000000000002 No Hit GGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTT 7 0.17500000000000002 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 7 0.17500000000000002 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 7 0.17500000000000002 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 7 0.17500000000000002 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 7 0.17500000000000002 No Hit CTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTAC 7 0.17500000000000002 No Hit GAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTT 7 0.17500000000000002 No Hit AGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATG 7 0.17500000000000002 No Hit AGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGC 7 0.17500000000000002 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 6 0.15 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 6 0.15 No Hit GCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATG 6 0.15 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 6 0.15 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 6 0.15 No Hit GAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAAT 6 0.15 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 6 0.15 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 5 0.125 No Hit GAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGA 5 0.125 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 5 0.125 No Hit ATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCA 5 0.125 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 5 0.125 No Hit ATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATAT 5 0.125 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 5 0.125 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 5 0.125 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 5 0.125 No Hit TCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGA 5 0.125 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 5 0.125 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 5 0.125 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 5 0.125 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 5 0.125 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 5 0.125 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 5 0.125 No Hit TGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCT 5 0.125 No Hit TCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACT 5 0.125 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 5 0.125 No Hit GAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTT 5 0.125 No Hit GAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.037500000000000006 0.0 0.0 0.0 0.0 88-89 0.05 0.0 0.0 0.0 0.0 90-91 0.05 0.0 0.0 0.0 0.0 92-93 0.075 0.0 0.0 0.0 0.0 94-95 0.075 0.0 0.0 0.0 0.0 96-97 0.1 0.0 0.0 0.0 0.0 98-99 0.16249999999999998 0.0 0.0 0.0 0.0 100-101 0.1875 0.0 0.0 0.0 0.0 102-103 0.25 0.0 0.0 0.0 0.0 104-105 0.3 0.0 0.0 0.0 0.0 106-107 0.36250000000000004 0.0 0.0 0.0 0.0 108-109 0.5 0.0 0.0 0.0 0.0 110-111 0.5874999999999999 0.0 0.0 0.0 0.0 112-113 0.6625 0.0 0.0 0.0 0.0 114-115 0.7875000000000001 0.0 0.0 0.0 0.0 116-117 0.825 0.0 0.0 0.0 0.0 118-119 0.9 0.0 0.0 0.0 0.0 120-121 1.1 0.0 0.0 0.0 0.0 122-123 1.15 0.0 0.0 0.0 0.0 124-125 1.225 0.0 0.0 0.0 0.0 126-127 1.35 0.0 0.0 0.0 0.0 128-129 1.4500000000000002 0.0 0.0 0.0 0.0 130-131 1.575 0.0 0.0 0.0 0.0 132-133 1.7374999999999998 0.0 0.0 0.0 0.0 134-135 1.95 0.0 0.0 0.0 0.0 136-137 2.05 0.0 0.0 0.0 0.0 138 2.05 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTA 10 0.006973645 144.0 9 ACGTTGC 10 0.006973645 144.0 5 TGGACGT 10 0.006973645 144.0 2 CGTTGCC 10 0.006973645 144.0 6 GTTGCCG 10 0.006973645 144.0 7 TTGCCGT 10 0.006973645 144.0 8 >>END_MODULE Read 2552264 spots for ERR11006594.sra Written 2552264 spots for ERR11006594.sra Read 2552264 spots for ERR11006594.sra Written 2552264 spots for ERR11006594.sra Read 2552264 spots for ERR11006594.sra Written 2552264 spots for ERR11006594.sra Read 2552264 spots for ERR11006594.sra Written 2552264 spots for ERR11006594.sra Read 2552264 spots for ERR11006594.sra Written 2552264 spots for ERR11006594.sra Read 2552264 spots for ERR11006594.sra Written 2552264 spots for ERR11006594.sra Read 2552264 spots for ERR11006594.sra Written 2552264 spots for ERR11006594.sra Read 2552264 spots for ERR11006594.sra Written 2552264 spots for ERR11006594.sra Read 2552264 spots for ERR11006594.sra Written 2552264 spots for ERR11006594.sra Read 2552264 spots for ERR11006594.sra Written 2552264 spots for ERR11006594.sra Read 2552264 spots for ERR11006594.sra Written 2552264 spots for ERR11006594.sra Read 2552264 spots for ERR11006594.sra Written 2552264 spots for ERR11006594.sra Read 2552264 spots for ERR11006594.sra Written 2552264 spots for ERR11006594.sra Read 2552264 spots for ERR11006594.sra Written 2552264 spots for ERR11006594.sra Read 2552264 spots for ERR11006594.sra Written 2552264 spots for ERR11006594.sra Read 2552264 spots for ERR11006594.sra Written 2552264 spots for ERR11006594.sra Read 2552264 spots for ERR11006594.sra Written 2552264 spots for ERR11006594.sra Read 2552272 spots for ERR11006594.sra Written 2552272 spots for ERR11006594.sra Read 2552264 spots for ERR11006594.sra Written 2552264 spots for ERR11006594.sra Read 2552264 spots for ERR11006594.sra Written 2552264 spots for ERR11006594.sra SRR ids: ['ERR11006594.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_1vrkugiz ERR11006594.sra spots: 51045288 blocks: [[1, 2552264], [2552265, 5104528], [5104529, 7656792], [7656793, 10209056], [10209057, 12761320], [12761321, 15313584], [15313585, 17865848], [17865849, 20418112], [20418113, 22970376], [22970377, 25522640], [25522641, 28074904], [28074905, 30627168], [30627169, 33179432], [33179433, 35731696], [35731697, 38283960], [38283961, 40836224], [40836225, 43388488], [43388489, 45940752], [45940753, 48493016], [48493017, 51045288]] ERR11006594 file size 18756159 ERR11006594 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006594 ERR11006594_1.fastq ERR11006594_2.fastq Input file: ERR11006594_1.fastq Paired file: ERR11006594_2.fastq trimmed: ERR11006594-trimmed-pair1.fastq, ERR11006594-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 19:28:58 2024 >> started Fri Dec 6 19:30:23 2024 >> done (84.959s) 51045288 read pairs processed; of these: 367 ( 0.00%) short read pairs filtered out after trimming by size control 1273 ( 0.00%) empty read pairs filtered out after trimming by size control 51043648 (100.00%) read pairs available; of these: 1765594 ( 3.46%) trimmed read pairs available after processing 49278054 (96.54%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 20 0.00% 19 18 0.00% 20 19 0.00% 21 24 0.00% 22 35 0.00% 23 22 0.00% 24 34 0.00% 25 1780 0.00% 26 24 0.00% 27 25 0.00% 28 25 0.00% 29 28 0.00% 30 25 0.00% 31 73 0.00% 32 34 0.00% 33 39 0.00% 34 25 0.00% 35 30 0.00% 36 32 0.00% 37 37 0.00% 38 39 0.00% 39 46 0.00% 40 39 0.00% 41 45 0.00% 42 49 0.00% 43 58 0.00% 44 55 0.00% 45 62 0.00% 46 55 0.00% 47 72 0.00% 48 56 0.00% 49 103 0.00% 50 83 0.00% 51 119 0.00% 52 123 0.00% 53 138 0.00% 54 129 0.00% 55 240 0.00% 56 155 0.00% 57 204 0.00% 58 209 0.00% 59 240 0.00% 60 258 0.00% 61 282 0.00% 62 355 0.00% 63 359 0.00% 64 379 0.00% 65 492 0.00% 66 493 0.00% 67 547 0.00% 68 551 0.00% 69 606 0.00% 70 684 0.00% 71 697 0.00% 72 836 0.00% 73 1006 0.00% 74 1143 0.00% 75 1324 0.00% 76 1328 0.00% 77 1469 0.00% 78 1553 0.00% 79 1680 0.00% 80 1781 0.00% 81 1932 0.00% 82 2177 0.00% 83 2333 0.00% 84 2553 0.01% 85 3051 0.01% 86 3410 0.01% 87 3683 0.01% 88 3932 0.01% 89 4146 0.01% 90 4172 0.01% 91 4462 0.01% 92 4491 0.01% 93 5219 0.01% 94 5296 0.01% 95 6080 0.01% 96 6394 0.01% 97 7068 0.01% 98 7493 0.01% 99 7856 0.02% 100 8128 0.02% 101 8740 0.02% 102 8864 0.02% 103 9118 0.02% 104 10049 0.02% 105 10553 0.02% 106 11898 0.02% 107 12446 0.02% 108 12929 0.03% 109 13819 0.03% 110 14270 0.03% 111 15038 0.03% 112 15922 0.03% 113 16084 0.03% 114 17076 0.03% 115 18066 0.04% 116 20540 0.04% 117 20925 0.04% 118 21185 0.04% 119 22917 0.04% 120 24312 0.05% 121 25128 0.05% 122 26463 0.05% 123 27009 0.05% 124 29091 0.06% 125 30720 0.06% 126 30314 0.06% 127 29991 0.06% 128 32975 0.06% 129 34167 0.07% 130 37583 0.07% 131 39594 0.08% 132 39456 0.08% 133 39848 0.08% 134 41824 0.08% 135 42145 0.08% 136 43810 0.09% 137 45444 0.09% 138 48298 0.09% 139 52368 0.10% 140 53878 0.11% 141 56824 0.11% 142 59803 0.12% 143 60084 0.12% 144 60986 0.12% 145 64763 0.13% 146 69421 0.14% 147 70821 0.14% 148 74587 0.15% 149 77081 0.15% 150 49278054 96.54% 51043648 reads passed initial QC criterion=sequence-density sequence-density=0.26 sequence-density-rank=1 fanout-score=2.54 fanout-score-rank=29 prefix-density=0.29 prefix-fanout=2.3 sequence=TACTTGTTCAAC criterion=fanout-score sequence-density=0.02 sequence-density-rank=33 fanout-score=31.08 fanout-score-rank=1 prefix-density=0.54 prefix-fanout=1.4 sequence=TCTTGCCAATAACCACGGCCGCTGAATAAAAACATTAAACTGAAGGCCCAGACAAAATGAGCACCTAAGAAAAAAAGACCATATGCAGATAATGAAGAACCATAAGACTGAATTACTTGCGATGCCTGTGCCCACAAGAAATCTCGAAGCCACCCATTAATCGTAATGGAACTCTGTGCAAAGTTCCCCCCTGTGATATGAGTTACCACCCCTTGATCACTTATAGTACCCCAAACATCCGATTGCATTTTCCAACTGAAATGGAAAATGACTACCGAAATTGCATTGTACATCCAGAATAAACCTAAGAAAACATGATCCCAAGCAGATACTTGACATGTTCCGCCTCGCCCAGGCCCATCGCAAGGAAAGCGAAAACCGAGATTTGCTTTATCGGGTATCAAACGGGAACTGCGAGCAAATAAAACACCTTTCAAAAGTATTAATACAGTCACATGGATGGTAAATGCGTGAATGTGATGGACTAAAAAATCTGCAGTTCCTAATGGAA criterion=sequence-density sequence-density=0.24 sequence-density-rank=1 fanout-score=1.95 fanout-score-rank=34 prefix-density=0.25 prefix-fanout=1.9 sequence=GTAGTAATTCTAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=37 fanout-score=124.37 fanout-score-rank=1 prefix-density=0.08 prefix-fanout=10.5 sequence=TTTTTTTTTATGAGATTTTTGCTAAAGTTTCATTTACGCCTAATTCACATCGAGTAGACCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAACTAAAGCAAGTGTTGGATTTAAAGCTGGTGTTAAAGATTATAGATTGACTTACTACACCCCGGAGTATGAAACCAAGGATACTGATATCTTGGCAGCATTCCGAGTATCTCCTCAACCTGGGGTTCCGCCCGAAGAAGCAGGGGCTGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTTTGGACTGATGGACTTACTAGTCTTGATCGTTACAAAGGACGATGCTATCACATCGAGCCTGTTCCTGGGGAAGACAGTCAATGGATCTGTTATGTAGCTTATCCATTAGATCTATTTGAAGAGGGTTCCGTTACTAACATGTTTACTTCCATTGTAGGTAACGTATTTGGTTTCAAAGCCCTACGTGCTCTACGTCTG ERR11006594 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 19:31:06 Started mapping on | Dec 06 19:31:06 Finished on | Dec 06 19:38:19 Mapping speed, Million of reads per hour | 424.38 Number of input reads | 51043648 Average input read length | 298 UNIQUE READS: Uniquely mapped reads number | 37667886 Uniquely mapped reads % | 73.80% Average mapped length | 297.52 Number of splices: Total | 18070836 Number of splices: Annotated (sjdb) | 17018353 Number of splices: GT/AG | 17719436 Number of splices: GC/AG | 206091 Number of splices: AT/AC | 25625 Number of splices: Non-canonical | 119684 Mismatch rate per base, % | 0.30% Deletion rate per base | 0.02% Deletion average length | 1.77 Insertion rate per base | 0.01% Insertion average length | 2.04 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 11125885 % of reads mapped to multiple loci | 21.80% Number of reads mapped to too many loci | 16388 % of reads mapped to too many loci | 0.03% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.66% % of reads unmapped: other | 0.72% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2249877 2249877 2249877 N_multimapping 11125885 11125885 11125885 N_noFeature 10010667 34868819 11975654 N_ambiguous 1319634 32054 480818 UnstrandedReadsAssigned:26337585 PositiveStrandReadsAssigned:2767013 NegativeStrandReadsAssigned:25211414 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006594 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006594-trimmed-pair1.fastq ERR11006594-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 51,043,648 reads, 32,158,642 reads pseudoaligned [quant] estimated average fragment length: 234.296 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,135 rounds 52973 ERR11006594.ke.tsv 35125 ERR11006594.se.tsv 88098 total ==> ERR11006594.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 702.848 0 0 PNS24247 1044 810.704 33.3081 1.43025 PNS24249 1928 1694.7 103.898 2.13423 PNS24246 1044 810.704 33.3081 1.43025 PNS24248 1044 810.704 33.3081 1.43025 PNS24244 1471 1237.7 83.1773 2.33945 PNS24243 293 71.8272 0 0 KQK14069 1603 1369.7 862.545 21.922 KQK14071 474 242.281 39.7595 5.71278 ==> ERR11006594.se.tsv <== BRADI_1g14170v3 980 BRADI_1g53295v3 2385 BRADI_1g59795v3 451 BRADI_1g07683v3 0 BRADI_1g00485v3 3 BRADI_1g20270v3 180 BRADI_1g74790v3 284 BRADI_1g09890v3 0 BRADI_1g77505v3 130 BRADI_1g48960v3 0 ERR11006594 completed mapping pipeline successfully