Starting /dee2/code/volunteer_pipeline.sh ERR11006595 current disk space = 1550175748096 free memory = 1598839276 ERR11006595 SRAfilesize 2faedb24d11d4d1e5b5cc307f5f9e170 ERR11006595.sra ERR11006595.sra file validated ERR11006595 is paired end ERR11006595 is conventional basespace ERR11006595 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006595_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5085 37.0 37.0 37.0 37.0 37.0 2 36.4275 37.0 37.0 37.0 37.0 37.0 3 36.584 37.0 37.0 37.0 37.0 37.0 4 36.617 37.0 37.0 37.0 37.0 37.0 5 36.548 37.0 37.0 37.0 37.0 37.0 6 36.515 37.0 37.0 37.0 37.0 37.0 7 36.59 37.0 37.0 37.0 37.0 37.0 8 36.4975 37.0 37.0 37.0 37.0 37.0 9 36.448 37.0 37.0 37.0 37.0 37.0 10-14 36.495 37.0 37.0 37.0 37.0 37.0 15-19 36.562 37.0 37.0 37.0 37.0 37.0 20-24 36.490700000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.4328 37.0 37.0 37.0 37.0 37.0 30-34 36.3986 37.0 37.0 37.0 37.0 37.0 35-39 36.3923 37.0 37.0 37.0 37.0 37.0 40-44 36.37779999999999 37.0 37.0 37.0 37.0 37.0 45-49 36.3235 37.0 37.0 37.0 37.0 37.0 50-54 36.331599999999995 37.0 37.0 37.0 37.0 37.0 55-59 36.341899999999995 37.0 37.0 37.0 37.0 37.0 60-64 36.2707 37.0 37.0 37.0 37.0 37.0 65-69 36.2604 37.0 37.0 37.0 37.0 37.0 70-74 36.1931 37.0 37.0 37.0 37.0 37.0 75-79 36.1631 37.0 37.0 37.0 37.0 37.0 80-84 36.1332 37.0 37.0 37.0 37.0 37.0 85-89 36.0904 37.0 37.0 37.0 37.0 37.0 90-94 36.0739 37.0 37.0 37.0 37.0 37.0 95-99 36.0298 37.0 37.0 37.0 37.0 37.0 100-104 36.0715 37.0 37.0 37.0 37.0 37.0 105-109 36.0433 37.0 37.0 37.0 37.0 37.0 110-114 35.887299999999996 37.0 37.0 37.0 37.0 37.0 115-119 35.9446 37.0 37.0 37.0 37.0 37.0 120-124 35.8313 37.0 37.0 37.0 37.0 37.0 125-129 35.7633 37.0 37.0 37.0 37.0 37.0 130-134 35.737399999999994 37.0 37.0 37.0 37.0 37.0 135-139 35.5783 37.0 37.0 37.0 37.0 37.0 140-144 35.495799999999996 37.0 37.0 37.0 37.0 37.0 145-149 35.544399999999996 37.0 37.0 37.0 37.0 37.0 150 35.3225 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 0.0 24 2.0 25 7.0 26 11.0 27 12.0 28 11.0 29 14.0 30 30.0 31 43.0 32 61.0 33 80.0 34 152.0 35 325.0 36 3014.0 37 237.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.91795897948975 15.307653826913455 15.43271635817909 33.34167083541771 2 29.9 12.5 26.625 30.975 3 23.724999999999998 14.825 26.775 34.675 4 27.775 19.5 23.325000000000003 29.4 5 27.3 23.974999999999998 23.025000000000002 25.7 6 25.7 31.874999999999996 20.275000000000002 22.15 7 14.625 31.724999999999998 35.0 18.65 8 15.475 31.4 33.525 19.6 9 17.5 27.975 35.15 19.375 10-14 19.225 32.235 27.075 21.465 15-19 19.375 33.395 25.44 21.790000000000003 20-24 18.81 30.564999999999998 27.625 23.0 25-29 21.57 31.845000000000002 25.795 20.79 30-34 21.975 31.130000000000003 25.96 20.935000000000002 35-39 22.14 32.035000000000004 24.555 21.27 40-44 19.645000000000003 31.175000000000004 26.005 23.175 45-49 20.225 29.95 27.515 22.31 50-54 19.994999999999997 33.54 24.935 21.529999999999998 55-59 21.865000000000002 30.955 23.895 23.285 60-64 20.205000000000002 31.045 26.174999999999997 22.575 65-69 20.330000000000002 30.7 25.86 23.11 70-74 21.01 30.635 24.490000000000002 23.865 75-79 21.815 29.875 25.895000000000003 22.415 80-84 20.905 30.264999999999997 25.71 23.119999999999997 85-89 22.74 29.925 25.665 21.67 90-94 21.2 31.115 26.135 21.55 95-99 22.475 30.930000000000003 24.115000000000002 22.48 100-104 20.875 31.490000000000002 24.990000000000002 22.645 105-109 21.605 29.970000000000002 26.340000000000003 22.085 110-114 21.785 29.75 25.575 22.89 115-119 19.74 31.595000000000002 25.85 22.814999999999998 120-124 20.415 30.53 24.765 24.29 125-129 20.57 31.36 23.415 24.654999999999998 130-134 21.915000000000003 31.825 24.68 21.58 135-139 22.355 30.915 23.135 23.595 140-144 22.855 30.78 25.040000000000003 21.325 145-149 22.085 31.525 24.425 21.965 150 22.85 28.7 25.5 22.95 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.5 21 2.0 22 2.0 23 3.0 24 5.0 25 5.5 26 12.5 27 18.5 28 18.5 29 23.0 30 20.5 31 24.0 32 29.0 33 30.0 34 42.5 35 63.0 36 110.0 37 200.0 38 290.0 39 281.0 40 260.0 41 292.5 42 281.5 43 257.5 44 220.0 45 215.0 46 202.5 47 140.5 48 121.5 49 113.5 50 91.0 51 65.5 52 42.5 53 32.0 54 38.5 55 33.5 56 25.0 57 27.5 58 21.5 59 20.0 60 25.5 61 28.0 62 24.5 63 23.0 64 27.5 65 27.5 66 19.5 67 12.0 68 12.5 69 13.0 70 13.0 71 17.0 72 16.5 73 11.0 74 9.5 75 11.5 76 9.5 77 4.5 78 4.5 79 3.5 80 1.0 81 0.5 82 1.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.95 #Duplication Level Percentage of deduplicated Percentage of total 1 82.41353936718176 56.00000000000001 2 10.559234731420162 14.35 3 2.6490066225165565 5.4 4 1.030169242089772 2.8000000000000003 5 1.0669610007358352 3.6249999999999996 6 0.4782928623988227 1.95 7 0.33112582781456956 1.575 8 0.40470934510669615 2.1999999999999997 9 0.11037527593818984 0.675 >10 0.919793966151582 9.1 >50 0.03679175864606328 2.325 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 93 2.325 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 27 0.675 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 25 0.625 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 24 0.6 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 23 0.575 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 21 0.525 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 19 0.475 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 16 0.4 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 14 0.35000000000000003 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 14 0.35000000000000003 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 14 0.35000000000000003 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 13 0.325 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 13 0.325 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 12 0.3 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 12 0.3 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 12 0.3 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 12 0.3 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 11 0.27499999999999997 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 11 0.27499999999999997 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 11 0.27499999999999997 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 10 0.25 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 10 0.25 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 10 0.25 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 10 0.25 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 10 0.25 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 10 0.25 No Hit TGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 9 0.22499999999999998 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 9 0.22499999999999998 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 9 0.22499999999999998 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 8 0.2 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 8 0.2 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 8 0.2 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 8 0.2 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 8 0.2 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 8 0.2 No Hit CACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCA 8 0.2 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 8 0.2 No Hit TAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGA 8 0.2 No Hit GGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGG 8 0.2 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 8 0.2 No Hit ACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTG 7 0.17500000000000002 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 7 0.17500000000000002 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 7 0.17500000000000002 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 7 0.17500000000000002 No Hit GCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCC 7 0.17500000000000002 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 7 0.17500000000000002 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 7 0.17500000000000002 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 7 0.17500000000000002 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 7 0.17500000000000002 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 6 0.15 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 6 0.15 No Hit TGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 6 0.15 No Hit CCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAA 6 0.15 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 6 0.15 No Hit TGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCT 6 0.15 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 6 0.15 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 6 0.15 No Hit GAGCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTA 6 0.15 No Hit CACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCG 6 0.15 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 6 0.15 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 6 0.15 No Hit GGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGCA 6 0.15 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 5 0.125 No Hit ACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGT 5 0.125 No Hit CCGCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGAT 5 0.125 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 5 0.125 No Hit AGGGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAAC 5 0.125 No Hit CAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAA 5 0.125 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 5 0.125 No Hit ACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATT 5 0.125 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 5 0.125 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 5 0.125 No Hit GGGTAAATCAAGAAAACAGCAGTCGCAGCTGCAACAGGAGCTGAATATGC 5 0.125 No Hit CCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTT 5 0.125 No Hit TATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 5 0.125 No Hit ATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCT 5 0.125 No Hit ATTGGGTAAAAGTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACC 5 0.125 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 5 0.125 No Hit CGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCT 5 0.125 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 5 0.125 No Hit GGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAG 5 0.125 No Hit GTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCA 5 0.125 No Hit ATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATA 5 0.125 No Hit CGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAAC 5 0.125 No Hit GCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAGCTGCAA 5 0.125 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 5 0.125 No Hit GGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGA 5 0.125 No Hit AGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCT 5 0.125 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 5 0.125 No Hit AGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTAT 5 0.125 No Hit CGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0125 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.0625 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.075 0.0 0.0 0.0 0.0 86-87 0.075 0.0 0.0 0.0 0.0 88-89 0.1 0.0 0.0 0.0 0.0 90-91 0.125 0.0 0.0 0.0 0.0 92-93 0.15 0.0 0.0 0.0 0.0 94-95 0.15 0.0 0.0 0.0 0.0 96-97 0.15 0.0 0.0 0.0 0.0 98-99 0.225 0.0 0.0 0.0 0.0 100-101 0.25 0.0 0.0 0.0 0.0 102-103 0.2875 0.0 0.0 0.0 0.0 104-105 0.3 0.0 0.0 0.0 0.0 106-107 0.35 0.0 0.0 0.0 0.0 108-109 0.4 0.0 0.0 0.0 0.0 110-111 0.425 0.0 0.0 0.0 0.0 112-113 0.4375 0.0 0.0 0.0 0.0 114-115 0.525 0.0 0.0 0.0 0.0 116-117 0.5875 0.0 0.0 0.0 0.0 118-119 0.6625 0.0 0.0 0.0 0.0 120-121 0.7749999999999999 0.0 0.0 0.0 0.0 122-123 0.8625 0.0 0.0 0.0 0.0 124-125 0.975 0.0 0.0 0.0 0.0 126-127 1.0875 0.0 0.0 0.0 0.0 128-129 1.1375 0.0 0.0 0.0 0.0 130-131 1.2375 0.0 0.0 0.0 0.0 132-133 1.3 0.0 0.0 0.0 0.0 134-135 1.4249999999999998 0.0 0.0 0.0 0.0 136-137 1.6 0.0 0.0 0.0 0.0 138 1.725 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCCTT 10 0.006973645 144.0 2 CTTTTCT 20 3.687869E-4 108.0 6 TTTTCTT 20 3.687869E-4 108.0 7 TTCTTTT 20 3.687869E-4 108.0 4 TCTTTTC 20 3.687869E-4 108.0 5 GCTTTCT 20 3.687869E-4 108.0 1 TTTCTTT 25 8.956223E-4 86.399994 3 CTTTCTT 25 8.956223E-4 86.399994 2 TTCTTCA 30 0.0018473949 72.0 9 TTTCTTC 35 0.0034045284 61.714283 8 ATATGTT 20 0.006139246 28.8 25-29 AAAATTC 20 0.006139246 28.8 15-19 >>END_MODULE ERR11006595 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006595_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2475 37.0 37.0 37.0 37.0 37.0 2 36.2905 37.0 37.0 37.0 37.0 37.0 3 36.547 37.0 37.0 37.0 37.0 37.0 4 36.32 37.0 37.0 37.0 37.0 37.0 5 36.5325 37.0 37.0 37.0 37.0 37.0 6 36.46 37.0 37.0 37.0 37.0 37.0 7 36.4285 37.0 37.0 37.0 37.0 37.0 8 36.522 37.0 37.0 37.0 37.0 37.0 9 36.54 37.0 37.0 37.0 37.0 37.0 10-14 36.4755 37.0 37.0 37.0 37.0 37.0 15-19 36.4625 37.0 37.0 37.0 37.0 37.0 20-24 36.4345 37.0 37.0 37.0 37.0 37.0 25-29 36.339999999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.295300000000005 37.0 37.0 37.0 37.0 37.0 35-39 36.27479999999999 37.0 37.0 37.0 37.0 37.0 40-44 36.287400000000005 37.0 37.0 37.0 37.0 37.0 45-49 36.2463 37.0 37.0 37.0 37.0 37.0 50-54 36.145799999999994 37.0 37.0 37.0 37.0 37.0 55-59 36.0719 37.0 37.0 37.0 37.0 37.0 60-64 36.0587 37.0 37.0 37.0 37.0 37.0 65-69 35.9932 37.0 37.0 37.0 37.0 37.0 70-74 35.903999999999996 37.0 37.0 37.0 37.0 37.0 75-79 35.8904 37.0 37.0 37.0 37.0 37.0 80-84 35.86579999999999 37.0 37.0 37.0 37.0 37.0 85-89 35.888 37.0 37.0 37.0 37.0 37.0 90-94 35.8798 37.0 37.0 37.0 37.0 37.0 95-99 35.77759999999999 37.0 37.0 37.0 37.0 37.0 100-104 35.7277 37.0 37.0 37.0 37.0 37.0 105-109 35.6443 37.0 37.0 37.0 37.0 37.0 110-114 35.65400000000001 37.0 37.0 37.0 37.0 37.0 115-119 35.6186 37.0 37.0 37.0 37.0 37.0 120-124 35.5817 37.0 37.0 37.0 37.0 37.0 125-129 35.3067 37.0 37.0 37.0 37.0 37.0 130-134 35.2483 37.0 37.0 37.0 29.8 37.0 135-139 35.2978 37.0 37.0 37.0 32.2 37.0 140-144 35.1014 37.0 37.0 37.0 25.0 37.0 145-149 34.988299999999995 37.0 37.0 37.0 32.2 37.0 150 35.0495 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 2.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.0 22 0.0 23 3.0 24 3.0 25 8.0 26 7.0 27 9.0 28 20.0 29 26.0 30 27.0 31 46.0 32 69.0 33 103.0 34 211.0 35 585.0 36 2696.0 37 183.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.499999999999996 25.324999999999996 18.65 27.525 2 25.6 26.3 33.85 14.249999999999998 3 21.224999999999998 28.725 32.35 17.7 4 23.724999999999998 31.3 25.7 19.275000000000002 5 24.7 32.25 26.125 16.925 6 20.125 35.099999999999994 27.825 16.950000000000003 7 18.525 20.175 40.725 20.575 8 20.25 22.775000000000002 32.5 24.474999999999998 9 22.7 20.125 34.475 22.7 10-14 23.31 26.784999999999997 29.115000000000002 20.79 15-19 23.48 25.590000000000003 30.37 20.560000000000002 20-24 23.41 25.395 30.56 20.635 25-29 22.66 25.215 31.105 21.02 30-34 23.799999999999997 25.14 31.485000000000003 19.575 35-39 23.14 25.05 30.675 21.135 40-44 22.99 26.009999999999998 30.29 20.71 45-49 22.91 27.46 29.654999999999998 19.975 50-54 22.57 26.38 29.630000000000003 21.42 55-59 22.400000000000002 26.235000000000003 30.34 21.025 60-64 22.215 25.380000000000003 30.245 22.16 65-69 22.27 26.284999999999997 30.520000000000003 20.925 70-74 23.65 25.05 30.94 20.36 75-79 23.125 25.240000000000002 31.069999999999997 20.565 80-84 23.330000000000002 26.240000000000002 30.36 20.07 85-89 23.745 25.445 29.275000000000002 21.535 90-94 22.994999999999997 24.905 31.195 20.905 95-99 23.375 24.47 30.654999999999998 21.5 100-104 23.285 24.845 31.385 20.485 105-109 24.240000000000002 24.834999999999997 30.18 20.745 110-114 23.1 24.88 30.470000000000002 21.55 115-119 23.62 25.345000000000002 30.380000000000003 20.655 120-124 22.905 25.7 29.2 22.195 125-129 22.285 25.585 30.115 22.015 130-134 22.6 25.52 30.345 21.535 135-139 23.155 25.515 31.490000000000002 19.84 140-144 23.395 26.150000000000002 29.755 20.7 145-149 23.115 25.255 30.37 21.26 150 22.45 25.25 30.55 21.75 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 3.0 21 4.5 22 2.5 23 3.0 24 7.0 25 14.5 26 17.5 27 14.5 28 21.0 29 30.0 30 39.5 31 45.5 32 38.0 33 45.0 34 63.0 35 79.5 36 124.5 37 196.0 38 232.0 39 225.0 40 264.0 41 276.5 42 233.5 43 243.5 44 258.0 45 236.5 46 189.0 47 141.5 48 114.5 49 85.5 50 64.5 51 59.0 52 48.5 53 41.0 54 33.5 55 26.5 56 28.5 57 31.0 58 30.0 59 24.5 60 28.0 61 33.0 62 29.0 63 22.5 64 27.0 65 31.0 66 24.5 67 20.0 68 19.5 69 18.0 70 17.5 71 13.0 72 9.5 73 10.5 74 10.0 75 11.0 76 9.5 77 7.0 78 4.5 79 3.0 80 2.5 81 3.0 82 2.5 83 2.5 84 2.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.025 #Duplication Level Percentage of deduplicated Percentage of total 1 81.13002535313292 56.00000000000001 2 10.865628395508873 15.0 3 2.825063382832307 5.8500000000000005 4 1.7747193045997829 4.9 5 1.0865628395508875 3.75 6 0.6157189424121695 2.55 7 0.6157189424121695 2.9749999999999996 8 0.2897500905469033 1.6 9 0.07243752263672583 0.44999999999999996 >10 0.7243752263672582 6.925000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 24 0.6 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 22 0.5499999999999999 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 18 0.44999999999999996 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 17 0.42500000000000004 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 17 0.42500000000000004 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 16 0.4 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 15 0.375 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 14 0.35000000000000003 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 14 0.35000000000000003 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 13 0.325 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 13 0.325 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 12 0.3 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 11 0.27499999999999997 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 11 0.27499999999999997 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 10 0.25 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 10 0.25 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 10 0.25 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 10 0.25 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 10 0.25 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 10 0.25 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 9 0.22499999999999998 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 9 0.22499999999999998 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 8 0.2 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 8 0.2 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 8 0.2 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 8 0.2 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 8 0.2 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 8 0.2 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 8 0.2 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 8 0.2 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 7 0.17500000000000002 No Hit AAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATC 7 0.17500000000000002 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 7 0.17500000000000002 No Hit AAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAG 7 0.17500000000000002 No Hit GATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTC 7 0.17500000000000002 No Hit GACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCC 7 0.17500000000000002 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 7 0.17500000000000002 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 7 0.17500000000000002 No Hit AAACAATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGAT 7 0.17500000000000002 No Hit TAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCC 7 0.17500000000000002 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 7 0.17500000000000002 No Hit AATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATG 7 0.17500000000000002 No Hit ATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTG 7 0.17500000000000002 No Hit CAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTG 7 0.17500000000000002 No Hit TGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACT 7 0.17500000000000002 No Hit GTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAAT 7 0.17500000000000002 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 7 0.17500000000000002 No Hit CTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGT 6 0.15 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 6 0.15 No Hit GTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCT 6 0.15 No Hit GAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGC 6 0.15 No Hit ATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATAT 6 0.15 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 6 0.15 No Hit CTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGC 6 0.15 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 6 0.15 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 6 0.15 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 6 0.15 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 6 0.15 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 6 0.15 No Hit CTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGG 6 0.15 No Hit TGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCT 6 0.15 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 6 0.15 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 6 0.15 No Hit GAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGT 6 0.15 No Hit CAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAAC 5 0.125 No Hit GTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTC 5 0.125 No Hit ATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCT 5 0.125 No Hit GTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACC 5 0.125 No Hit ATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGG 5 0.125 No Hit CGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGAC 5 0.125 No Hit CAATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAA 5 0.125 No Hit GAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTC 5 0.125 No Hit CTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATT 5 0.125 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 5 0.125 No Hit GTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCAT 5 0.125 No Hit CTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAG 5 0.125 No Hit AATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGC 5 0.125 No Hit GTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTAT 5 0.125 No Hit AGGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACT 5 0.125 No Hit TCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGT 5 0.125 No Hit TTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTCACATGTTA 5 0.125 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 5 0.125 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 5 0.125 No Hit ATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAG 5 0.125 No Hit GGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTT 5 0.125 No Hit GAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAAT 5 0.125 No Hit AACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGG 5 0.125 No Hit TACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGG 5 0.125 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 5 0.125 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 5 0.125 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 5 0.125 No Hit AGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATG 5 0.125 No Hit AGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGC 5 0.125 No Hit TGCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0125 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.0625 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.075 0.0 0.0 0.0 0.0 86-87 0.075 0.0 0.0 0.0 0.0 88-89 0.1 0.0 0.0 0.0 0.0 90-91 0.1 0.0 0.0 0.0 0.0 92-93 0.1 0.0 0.0 0.0 0.0 94-95 0.1 0.0 0.0 0.0 0.0 96-97 0.1 0.0 0.0 0.0 0.0 98-99 0.175 0.0 0.0 0.0 0.0 100-101 0.2 0.0 0.0 0.0 0.0 102-103 0.2375 0.0 0.0 0.0 0.0 104-105 0.25 0.0 0.0 0.0 0.0 106-107 0.30000000000000004 0.0 0.0 0.0 0.0 108-109 0.35 0.0 0.0 0.0 0.0 110-111 0.375 0.0 0.0 0.0 0.0 112-113 0.4 0.0 0.0 0.0 0.0 114-115 0.5 0.0 0.0 0.0 0.0 116-117 0.5625 0.0 0.0 0.0 0.0 118-119 0.6375 0.0 0.0 0.0 0.0 120-121 0.75 0.0 0.0 0.0 0.0 122-123 0.8374999999999999 0.0 0.0 0.0 0.0 124-125 0.95 0.0 0.0 0.0 0.0 126-127 1.0625 0.0 0.0 0.0 0.0 128-129 1.1125 0.0 0.0 0.0 0.0 130-131 1.2125 0.0 0.0 0.0 0.0 132-133 1.275 0.0 0.0 0.0 0.0 134-135 1.4 0.0 0.0 0.0 0.0 136-137 1.5625 0.0 0.0 0.0 0.0 138 1.675 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 2537261 spots for ERR11006595.sra Written 2537261 spots for ERR11006595.sra Read 2537261 spots for ERR11006595.sra Written 2537261 spots for ERR11006595.sra Read 2537261 spots for ERR11006595.sra Written 2537261 spots for ERR11006595.sra Read 2537261 spots for ERR11006595.sra Written 2537261 spots for ERR11006595.sra Read 2537261 spots for ERR11006595.sra Written 2537261 spots for ERR11006595.sra Read 2537261 spots for ERR11006595.sra Written 2537261 spots for ERR11006595.sra Read 2537261 spots for ERR11006595.sra Written 2537261 spots for ERR11006595.sra Read 2537261 spots for ERR11006595.sra Written 2537261 spots for ERR11006595.sra Read 2537261 spots for ERR11006595.sra Written 2537261 spots for ERR11006595.sra Read 2537261 spots for ERR11006595.sra Written 2537261 spots for ERR11006595.sra Read 2537261 spots for ERR11006595.sra Written 2537261 spots for ERR11006595.sra Read 2537261 spots for ERR11006595.sra Written 2537261 spots for ERR11006595.sra Read 2537261 spots for ERR11006595.sra Written 2537261 spots for ERR11006595.sra Read 2537278 spots for ERR11006595.sra Written 2537278 spots for ERR11006595.sra Read 2537261 spots for ERR11006595.sra Written 2537261 spots for ERR11006595.sra Read 2537261 spots for ERR11006595.sra Written 2537261 spots for ERR11006595.sra Read 2537261 spots for ERR11006595.sra Written 2537261 spots for ERR11006595.sra Read 2537261 spots for ERR11006595.sra Written 2537261 spots for ERR11006595.sra Read 2537261 spots for ERR11006595.sra Written 2537261 spots for ERR11006595.sra Read 2537261 spots for ERR11006595.sra Written 2537261 spots for ERR11006595.sra SRR ids: ['ERR11006595.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_i9_9sieh ERR11006595.sra spots: 50745237 blocks: [[1, 2537261], [2537262, 5074522], [5074523, 7611783], [7611784, 10149044], [10149045, 12686305], [12686306, 15223566], [15223567, 17760827], [17760828, 20298088], [20298089, 22835349], [22835350, 25372610], [25372611, 27909871], [27909872, 30447132], [30447133, 32984393], [32984394, 35521654], [35521655, 38058915], [38058916, 40596176], [40596177, 43133437], [43133438, 45670698], [45670699, 48207959], [48207960, 50745237]] ERR11006595 file size 18645776 ERR11006595 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006595 ERR11006595_1.fastq ERR11006595_2.fastq Input file: ERR11006595_1.fastq Paired file: ERR11006595_2.fastq trimmed: ERR11006595-trimmed-pair1.fastq, ERR11006595-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 18:53:19 2024 >> started Fri Dec 6 18:54:17 2024 >> done (58.067s) 50745237 read pairs processed; of these: 478 ( 0.00%) short read pairs filtered out after trimming by size control 2300 ( 0.00%) empty read pairs filtered out after trimming by size control 50742459 (99.99%) read pairs available; of these: 1592944 ( 3.14%) trimmed read pairs available after processing 49149515 (96.86%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 20 0.00% 19 19 0.00% 20 27 0.00% 21 31 0.00% 22 48 0.00% 23 17 0.00% 24 34 0.00% 25 1537 0.00% 26 38 0.00% 27 37 0.00% 28 37 0.00% 29 40 0.00% 30 43 0.00% 31 63 0.00% 32 30 0.00% 33 32 0.00% 34 35 0.00% 35 46 0.00% 36 40 0.00% 37 37 0.00% 38 42 0.00% 39 46 0.00% 40 54 0.00% 41 63 0.00% 42 65 0.00% 43 74 0.00% 44 75 0.00% 45 66 0.00% 46 61 0.00% 47 93 0.00% 48 102 0.00% 49 110 0.00% 50 92 0.00% 51 108 0.00% 52 121 0.00% 53 158 0.00% 54 176 0.00% 55 255 0.00% 56 189 0.00% 57 227 0.00% 58 211 0.00% 59 233 0.00% 60 282 0.00% 61 305 0.00% 62 338 0.00% 63 400 0.00% 64 439 0.00% 65 518 0.00% 66 506 0.00% 67 564 0.00% 68 656 0.00% 69 610 0.00% 70 673 0.00% 71 706 0.00% 72 837 0.00% 73 989 0.00% 74 1131 0.00% 75 1340 0.00% 76 1365 0.00% 77 1484 0.00% 78 1601 0.00% 79 1597 0.00% 80 1767 0.00% 81 2093 0.00% 82 2147 0.00% 83 2371 0.00% 84 2518 0.00% 85 3036 0.01% 86 3285 0.01% 87 3549 0.01% 88 3778 0.01% 89 4059 0.01% 90 4361 0.01% 91 4334 0.01% 92 4517 0.01% 93 5184 0.01% 94 5257 0.01% 95 6036 0.01% 96 6335 0.01% 97 6850 0.01% 98 7212 0.01% 99 7384 0.01% 100 7940 0.02% 101 8051 0.02% 102 8552 0.02% 103 8723 0.02% 104 9519 0.02% 105 10262 0.02% 106 11254 0.02% 107 11630 0.02% 108 11953 0.02% 109 12611 0.02% 110 13619 0.03% 111 14020 0.03% 112 14937 0.03% 113 14498 0.03% 114 15700 0.03% 115 16698 0.03% 116 18903 0.04% 117 18765 0.04% 118 19754 0.04% 119 20616 0.04% 120 22291 0.04% 121 23177 0.05% 122 24689 0.05% 123 24804 0.05% 124 26628 0.05% 125 28228 0.06% 126 26999 0.05% 127 27046 0.05% 128 29457 0.06% 129 31047 0.06% 130 34024 0.07% 131 36455 0.07% 132 36711 0.07% 133 35125 0.07% 134 37087 0.07% 135 36775 0.07% 136 38518 0.08% 137 40226 0.08% 138 42471 0.08% 139 45277 0.09% 140 46800 0.09% 141 50442 0.10% 142 51598 0.10% 143 52408 0.10% 144 53085 0.10% 145 57291 0.11% 146 63301 0.12% 147 62301 0.12% 148 65631 0.13% 149 67801 0.13% 150 49149515 96.86% 50742459 reads passed initial QC criterion=sequence-density sequence-density=0.23 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=30 prefix-density=0.22 prefix-fanout=2.0 sequence=TCTAATTCAAAA criterion=fanout-score sequence-density=0.11 sequence-density-rank=12 fanout-score=70.60 fanout-score-rank=1 prefix-density=7.21 prefix-fanout=1.0 sequence=GCGATGAAGCTGATGCACTGCACCTGCC criterion=sequence-density sequence-density=0.22 sequence-density-rank=1 fanout-score=1.00 fanout-score-rank=41 prefix-density=0.02 prefix-fanout=1.0 sequence=GGGGGGGGGGATGTAAGAAAAATAACCAACTTGACCCTTAGCCCCGGTGTTATATTTGGTTATTTACTAAAATCCCCTTTTGGGGGAGAAGGCTGGATTGTTAGTGTGGATGATTTAGAAGATATAATTGGTGGACATGTATGGTTGGGTTTCATTTGTGTATTTGGCGGAATTTGGCATATCTTAACCAAACCTTTCGCATGGGCTCGCCGTGCATTTGTATGGTCTGGAGAAGCTTACTTGTCTTATAGTTTAGCTGCTTTATCTGTCTTTGGTTTTATCGCTTGTTGTTTTGTCTGGTTCAATAATACGGCTTATCCGAGTGAGTTTTATGGACCCACCGGCCCAGAAGCTTCTCAAGCTCAAGCATTTACTTTTCTAGTTAGAGACCAGCGTCTTGGAGCTAATGTGGGATCTGCTCAAGGACCCACAGGTTTAGGTAAATATCTAATGCGTTCCCCAACGGGAGAGGTTATCTTTGGAGGGGAAACTATGCGTTTTTGGGACCTCC criterion=fanout-score sequence-density=0.09 sequence-density-rank=20 fanout-score=85.31 fanout-score-rank=1 prefix-density=7.68 prefix-fanout=1.0 sequence=ATAACTAGCACAGAAAATCGTCT ERR11006595 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 18:55:00 Started mapping on | Dec 06 18:55:00 Finished on | Dec 06 19:00:51 Mapping speed, Million of reads per hour | 520.44 Number of input reads | 50742459 Average input read length | 298 UNIQUE READS: Uniquely mapped reads number | 36262720 Uniquely mapped reads % | 71.46% Average mapped length | 297.71 Number of splices: Total | 13075881 Number of splices: Annotated (sjdb) | 12260724 Number of splices: GT/AG | 12771072 Number of splices: GC/AG | 150029 Number of splices: AT/AC | 24969 Number of splices: Non-canonical | 129811 Mismatch rate per base, % | 0.29% Deletion rate per base | 0.01% Deletion average length | 1.67 Insertion rate per base | 0.01% Insertion average length | 2.05 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 12911781 % of reads mapped to multiple loci | 25.45% Number of reads mapped to too many loci | 7139 % of reads mapped to too many loci | 0.01% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.71% % of reads unmapped: other | 0.36% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1567958 1567958 1567958 N_multimapping 12911781 12911781 12911781 N_noFeature 11297749 34013891 12735239 N_ambiguous 1386769 33999 578039 UnstrandedReadsAssigned:23578202 PositiveStrandReadsAssigned:2214830 NegativeStrandReadsAssigned:22949442 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006595 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006595-trimmed-pair1.fastq ERR11006595-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 50,742,459 reads, 30,673,908 reads pseudoaligned [quant] estimated average fragment length: 237.575 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,111 rounds 52973 ERR11006595.ke.tsv 35125 ERR11006595.se.tsv 88098 total ==> ERR11006595.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 699.727 1.96144 0.0948426 PNS24247 1044 807.425 19.9852 0.837456 PNS24249 1928 1691.42 56.0302 1.1208 PNS24246 1044 807.425 19.9852 0.837456 PNS24248 1044 807.425 19.9852 0.837456 PNS24244 1471 1234.42 58.0529 1.59117 PNS24243 293 70.3463 1 0.480967 KQK14069 1603 1366.42 827.88 20.4993 KQK14071 474 239.288 17.7871 2.51502 ==> ERR11006595.se.tsv <== BRADI_1g14170v3 939 BRADI_1g53295v3 1175 BRADI_1g59795v3 310 BRADI_1g07683v3 0 BRADI_1g00485v3 2 BRADI_1g20270v3 158 BRADI_1g74790v3 192 BRADI_1g09890v3 0 BRADI_1g77505v3 115 BRADI_1g48960v3 0 ERR11006595 completed mapping pipeline successfully