Starting /dee2/code/volunteer_pipeline.sh ERR11006596 current disk space = 1550203654144 free memory = 1598900276 ERR11006596 SRAfilesize 2e03e72ceddcf370aae1764206462e93 ERR11006596.sra ERR11006596.sra file validated ERR11006596 is paired end ERR11006596 is conventional basespace ERR11006596 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006596_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5175 37.0 37.0 37.0 37.0 37.0 2 36.4175 37.0 37.0 37.0 37.0 37.0 3 36.5185 37.0 37.0 37.0 37.0 37.0 4 36.602 37.0 37.0 37.0 37.0 37.0 5 36.6315 37.0 37.0 37.0 37.0 37.0 6 36.5365 37.0 37.0 37.0 37.0 37.0 7 36.572 37.0 37.0 37.0 37.0 37.0 8 36.5325 37.0 37.0 37.0 37.0 37.0 9 36.58 37.0 37.0 37.0 37.0 37.0 10-14 36.4417 37.0 37.0 37.0 37.0 37.0 15-19 36.5484 37.0 37.0 37.0 37.0 37.0 20-24 36.4679 37.0 37.0 37.0 37.0 37.0 25-29 36.4026 37.0 37.0 37.0 37.0 37.0 30-34 36.38270000000001 37.0 37.0 37.0 37.0 37.0 35-39 36.296800000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.2672 37.0 37.0 37.0 37.0 37.0 45-49 36.2667 37.0 37.0 37.0 37.0 37.0 50-54 36.2132 37.0 37.0 37.0 37.0 37.0 55-59 36.245400000000004 37.0 37.0 37.0 37.0 37.0 60-64 36.174 37.0 37.0 37.0 37.0 37.0 65-69 36.1774 37.0 37.0 37.0 37.0 37.0 70-74 36.1488 37.0 37.0 37.0 37.0 37.0 75-79 36.068400000000004 37.0 37.0 37.0 37.0 37.0 80-84 36.079499999999996 37.0 37.0 37.0 37.0 37.0 85-89 35.995799999999996 37.0 37.0 37.0 37.0 37.0 90-94 35.994299999999996 37.0 37.0 37.0 37.0 37.0 95-99 35.921800000000005 37.0 37.0 37.0 37.0 37.0 100-104 35.9704 37.0 37.0 37.0 37.0 37.0 105-109 35.898900000000005 37.0 37.0 37.0 37.0 37.0 110-114 35.737300000000005 37.0 37.0 37.0 37.0 37.0 115-119 35.725 37.0 37.0 37.0 37.0 37.0 120-124 35.6041 37.0 37.0 37.0 37.0 37.0 125-129 35.624700000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.6074 37.0 37.0 37.0 37.0 37.0 135-139 35.445299999999996 37.0 37.0 37.0 37.0 37.0 140-144 35.3212 37.0 37.0 37.0 34.6 37.0 145-149 35.45309999999999 37.0 37.0 37.0 37.0 37.0 150 35.207 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 2.0 22 1.0 23 3.0 24 7.0 25 14.0 26 7.0 27 9.0 28 15.0 29 33.0 30 32.0 31 41.0 32 66.0 33 105.0 34 138.0 35 323.0 36 2944.0 37 260.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.3671835917959 13.606803401700851 15.607803901950975 36.418209104552275 2 26.6 12.049999999999999 30.975 30.375000000000004 3 21.025 14.45 29.4 35.125 4 28.325 18.8 22.8 30.075000000000003 5 28.1 23.375 24.75 23.775 6 24.349999999999998 30.325000000000003 21.525 23.799999999999997 7 16.650000000000002 29.2 34.675 19.475 8 17.724999999999998 29.4 32.324999999999996 20.549999999999997 9 18.325 26.5 34.575 20.599999999999998 10-14 20.36 31.205 26.314999999999998 22.12 15-19 20.925 31.505 25.355 22.215 20-24 20.31 29.015 27.224999999999998 23.45 25-29 22.235 30.459999999999997 25.929999999999996 21.375 30-34 22.975 29.909999999999997 25.47 21.645 35-39 21.705 30.385 25.424999999999997 22.485 40-44 21.035 30.4 25.895000000000003 22.67 45-49 21.005 29.24 26.540000000000003 23.215 50-54 21.240000000000002 30.959999999999997 25.55 22.25 55-59 21.87 29.38 24.52 24.23 60-64 21.83 30.075000000000003 25.19 22.905 65-69 21.740000000000002 29.09 25.53 23.64 70-74 22.5 29.265 24.22 24.015 75-79 22.085 29.82 24.42 23.674999999999997 80-84 22.939999999999998 28.610000000000003 25.074999999999996 23.375 85-89 23.125 29.095 24.865000000000002 22.915 90-94 21.465 29.14 25.929999999999996 23.465 95-99 22.745 29.360000000000003 24.335 23.56 100-104 21.215 30.904999999999998 24.83 23.05 105-109 21.94 28.005000000000003 25.825 24.23 110-114 22.705000000000002 28.315 25.535000000000004 23.445 115-119 20.925 29.205 25.509999999999998 24.36 120-124 21.82 29.125 24.855 24.2 125-129 21.015 30.435000000000002 23.825 24.725 130-134 22.585 30.2 24.645 22.57 135-139 23.465 28.96 24.11 23.465 140-144 23.47 28.48 25.564999999999998 22.485 145-149 22.689999999999998 29.95 24.025 23.335 150 23.400000000000002 28.749999999999996 25.424999999999997 22.425 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 2.0 6 1.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 1.0 19 2.0 20 1.5 21 0.5 22 0.5 23 1.0 24 2.0 25 3.5 26 6.5 27 7.5 28 13.5 29 14.5 30 16.0 31 26.5 32 35.0 33 44.0 34 56.0 35 68.5 36 88.5 37 168.5 38 248.5 39 244.5 40 234.5 41 249.5 42 233.0 43 216.0 44 215.5 45 214.0 46 212.0 47 162.0 48 109.0 49 112.5 50 103.5 51 73.5 52 68.5 53 57.0 54 42.0 55 43.0 56 40.0 57 33.0 58 27.0 59 31.5 60 32.0 61 23.5 62 27.0 63 31.0 64 42.5 65 61.0 66 45.0 67 21.0 68 21.0 69 22.0 70 19.5 71 20.5 72 21.5 73 15.5 74 14.0 75 14.5 76 9.0 77 7.5 78 7.0 79 4.0 80 4.5 81 3.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.125 #Duplication Level Percentage of deduplicated Percentage of total 1 84.45923460898503 63.449999999999996 2 10.615640599001663 15.950000000000001 3 2.0632279534109816 4.65 4 1.0316139767054908 3.1 5 0.5324459234608985 2.0 6 0.36605657237936773 1.6500000000000001 7 0.16638935108153077 0.8750000000000001 8 0.09983361064891848 0.6 9 0.09983361064891848 0.675 >10 0.5324459234608985 5.2749999999999995 >50 0.033277870216306155 1.775 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 71 1.775 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 24 0.6 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 20 0.5 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 17 0.42500000000000004 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 14 0.35000000000000003 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 14 0.35000000000000003 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 13 0.325 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 12 0.3 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 12 0.3 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 12 0.3 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 12 0.3 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 11 0.27499999999999997 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 10 0.25 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 10 0.25 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 10 0.25 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 10 0.25 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 10 0.25 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 9 0.22499999999999998 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 9 0.22499999999999998 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 9 0.22499999999999998 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 8 0.2 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 8 0.2 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 8 0.2 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 7 0.17500000000000002 No Hit TCTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTA 7 0.17500000000000002 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 7 0.17500000000000002 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 7 0.17500000000000002 No Hit TAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGA 7 0.17500000000000002 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 6 0.15 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 6 0.15 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 6 0.15 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 6 0.15 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 6 0.15 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 6 0.15 No Hit TCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGT 6 0.15 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 6 0.15 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 6 0.15 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 6 0.15 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 6 0.15 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 5 0.125 No Hit TCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCG 5 0.125 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 5 0.125 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 5 0.125 No Hit CAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGG 5 0.125 No Hit GCCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 5 0.125 No Hit TGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGA 5 0.125 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 5 0.125 No Hit TGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCT 5 0.125 No Hit ATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATA 5 0.125 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 5 0.125 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 5 0.125 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 5 0.125 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 5 0.125 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 5 0.125 No Hit CTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.025 0.0 48-49 0.0 0.0 0.0 0.025 0.0 50-51 0.0 0.0 0.0 0.025 0.0 52-53 0.0 0.0 0.0 0.025 0.0 54-55 0.0125 0.0 0.0 0.025 0.0 56-57 0.025 0.0 0.0 0.025 0.0 58-59 0.025 0.0 0.0 0.025 0.0 60-61 0.025 0.0 0.0 0.025 0.0 62-63 0.025 0.0 0.0 0.025 0.0 64-65 0.025 0.0 0.0 0.025 0.0 66-67 0.025 0.0 0.0 0.025 0.0 68-69 0.025 0.0 0.0 0.025 0.0 70-71 0.025 0.0 0.0 0.025 0.0 72-73 0.025 0.0 0.0 0.025 0.0 74-75 0.025 0.0 0.0 0.025 0.0 76-77 0.025 0.0 0.0 0.025 0.0 78-79 0.037500000000000006 0.0 0.0 0.025 0.0 80-81 0.05 0.0 0.0 0.025 0.0 82-83 0.075 0.0 0.0 0.025 0.0 84-85 0.075 0.0 0.0 0.025 0.0 86-87 0.075 0.0 0.0 0.025 0.0 88-89 0.075 0.0 0.0 0.025 0.0 90-91 0.0875 0.0 0.0 0.025 0.0 92-93 0.1 0.0 0.0 0.025 0.0 94-95 0.125 0.0 0.0 0.025 0.0 96-97 0.125 0.0 0.0 0.025 0.0 98-99 0.125 0.0 0.0 0.025 0.0 100-101 0.15 0.0 0.0 0.025 0.0 102-103 0.15 0.0 0.0 0.025 0.0 104-105 0.15 0.0 0.0 0.025 0.0 106-107 0.15 0.0 0.0 0.025 0.0 108-109 0.16249999999999998 0.0 0.0 0.025 0.0 110-111 0.1875 0.0 0.0 0.025 0.0 112-113 0.21250000000000002 0.0 0.0 0.025 0.0 114-115 0.275 0.0 0.0 0.025 0.0 116-117 0.275 0.0 0.0 0.025 0.0 118-119 0.3375 0.0 0.0 0.025 0.0 120-121 0.4125 0.0 0.0 0.025 0.0 122-123 0.5 0.0 0.0 0.025 0.0 124-125 0.55 0.0 0.0 0.025 0.0 126-127 0.6625000000000001 0.0 0.0 0.025 0.0 128-129 0.75 0.0 0.0 0.025 0.0 130-131 0.8125 0.0 0.0 0.025 0.0 132-133 0.85 0.0 0.0 0.025 0.0 134-135 0.9625 0.0 0.0 0.025 0.0 136-137 1.1 0.0 0.0 0.025 0.0 138 1.125 0.0 0.0 0.025 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGAAGT 10 0.006973645 144.0 2 >>END_MODULE ERR11006596 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006596_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.161 37.0 37.0 37.0 37.0 37.0 2 36.1635 37.0 37.0 37.0 37.0 37.0 3 36.3915 37.0 37.0 37.0 37.0 37.0 4 36.328 37.0 37.0 37.0 37.0 37.0 5 36.4985 37.0 37.0 37.0 37.0 37.0 6 36.391 37.0 37.0 37.0 37.0 37.0 7 36.489 37.0 37.0 37.0 37.0 37.0 8 36.532 37.0 37.0 37.0 37.0 37.0 9 36.362 37.0 37.0 37.0 37.0 37.0 10-14 36.4162 37.0 37.0 37.0 37.0 37.0 15-19 36.4277 37.0 37.0 37.0 37.0 37.0 20-24 36.369099999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.3021 37.0 37.0 37.0 37.0 37.0 30-34 36.21169999999999 37.0 37.0 37.0 37.0 37.0 35-39 36.2662 37.0 37.0 37.0 37.0 37.0 40-44 36.287400000000005 37.0 37.0 37.0 37.0 37.0 45-49 36.2639 37.0 37.0 37.0 37.0 37.0 50-54 36.1355 37.0 37.0 37.0 37.0 37.0 55-59 36.0589 37.0 37.0 37.0 37.0 37.0 60-64 36.0649 37.0 37.0 37.0 37.0 37.0 65-69 35.9865 37.0 37.0 37.0 37.0 37.0 70-74 35.9792 37.0 37.0 37.0 37.0 37.0 75-79 35.8365 37.0 37.0 37.0 37.0 37.0 80-84 35.814099999999996 37.0 37.0 37.0 37.0 37.0 85-89 35.851800000000004 37.0 37.0 37.0 37.0 37.0 90-94 35.824 37.0 37.0 37.0 37.0 37.0 95-99 35.7869 37.0 37.0 37.0 37.0 37.0 100-104 35.6676 37.0 37.0 37.0 37.0 37.0 105-109 35.663 37.0 37.0 37.0 37.0 37.0 110-114 35.6515 37.0 37.0 37.0 37.0 37.0 115-119 35.4726 37.0 37.0 37.0 34.6 37.0 120-124 35.4983 37.0 37.0 37.0 37.0 37.0 125-129 35.378 37.0 37.0 37.0 37.0 37.0 130-134 35.168400000000005 37.0 37.0 37.0 29.8 37.0 135-139 35.2065 37.0 37.0 37.0 29.8 37.0 140-144 35.0976 37.0 37.0 37.0 25.0 37.0 145-149 34.95649999999999 37.0 37.0 37.0 27.4 37.0 150 34.859 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 4.0 23 2.0 24 7.0 25 5.0 26 8.0 27 14.0 28 17.0 29 20.0 30 33.0 31 41.0 32 69.0 33 131.0 34 204.0 35 619.0 36 2629.0 37 196.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.425 23.625 17.05 30.9 2 25.525 26.325 32.625 15.525 3 20.7 28.825 31.25 19.225 4 24.25 28.975 27.025 19.75 5 24.65 31.3 25.874999999999996 18.175 6 21.05 35.275 25.25 18.425 7 21.575 20.0 37.225 21.2 8 21.775 24.7 30.8 22.725 9 23.225 21.4 32.375 23.0 10-14 24.240000000000002 26.040000000000003 27.74 21.98 15-19 24.065 25.405 29.17 21.36 20-24 23.825 25.14 29.459999999999997 21.575 25-29 23.905 25.185000000000002 29.48 21.43 30-34 24.355 25.165 29.9 20.580000000000002 35-39 24.385 24.77 29.12 21.725 40-44 23.7 25.105 29.255 21.94 45-49 23.895 26.095000000000002 27.944999999999997 22.065 50-54 23.695 25.69 28.854999999999997 21.759999999999998 55-59 23.794999999999998 24.9 29.265 22.040000000000003 60-64 23.565 25.25 29.160000000000004 22.025 65-69 23.87 25.069999999999997 28.965000000000003 22.095000000000002 70-74 24.45 24.63 29.189999999999998 21.73 75-79 24.11 24.815 29.195 21.88 80-84 23.93 24.82 29.67 21.58 85-89 24.39 25.575 27.72 22.314999999999998 90-94 24.16 24.585 29.049999999999997 22.205 95-99 23.82 25.040000000000003 28.895 22.245 100-104 23.74 24.425 29.975 21.86 105-109 24.64 24.445 29.185 21.73 110-114 23.64 25.174999999999997 28.62 22.564999999999998 115-119 24.13 25.840000000000003 28.525 21.505 120-124 24.205 25.330000000000002 27.77 22.695 125-129 23.02 25.25 29.134999999999998 22.595000000000002 130-134 23.505000000000003 25.385 29.099999999999998 22.009999999999998 135-139 24.060000000000002 25.080000000000002 29.160000000000004 21.7 140-144 24.474999999999998 25.005 29.104999999999997 21.415 145-149 23.919999999999998 25.3 28.535 22.245 150 24.325 24.025 29.5 22.15 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.5 19 1.0 20 1.5 21 3.5 22 4.0 23 2.5 24 2.0 25 5.5 26 9.0 27 11.5 28 14.5 29 21.0 30 28.0 31 37.5 32 45.5 33 49.5 34 59.0 35 71.0 36 110.0 37 165.5 38 189.0 39 200.5 40 218.0 41 232.0 42 225.0 43 223.5 44 231.0 45 204.0 46 181.5 47 160.0 48 132.5 49 110.5 50 84.5 51 70.0 52 59.5 53 55.0 54 55.5 55 46.5 56 35.5 57 31.0 58 29.0 59 31.5 60 32.0 61 38.0 62 39.5 63 45.0 64 57.5 65 49.5 66 35.0 67 31.5 68 29.0 69 24.0 70 24.0 71 22.5 72 20.0 73 18.5 74 22.5 75 16.0 76 10.0 77 10.5 78 7.0 79 5.0 80 5.0 81 3.5 82 1.0 83 0.5 84 0.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.4 #Duplication Level Percentage of deduplicated Percentage of total 1 83.37696335078533 63.7 2 11.12565445026178 17.0 3 2.5523560209424083 5.8500000000000005 4 1.0143979057591623 3.1 5 0.68717277486911 2.625 6 0.42539267015706805 1.95 7 0.1963350785340314 1.05 8 0.2617801047120419 1.6 9 0.0981675392670157 0.675 >10 0.2617801047120419 2.45 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 15 0.375 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 14 0.35000000000000003 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 12 0.3 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 12 0.3 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 12 0.3 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 12 0.3 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 11 0.27499999999999997 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 10 0.25 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 9 0.22499999999999998 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 9 0.22499999999999998 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 9 0.22499999999999998 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 8 0.2 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 8 0.2 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 8 0.2 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 8 0.2 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 8 0.2 No Hit CTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAG 8 0.2 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 8 0.2 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 8 0.2 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 7 0.17500000000000002 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 7 0.17500000000000002 No Hit CCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGA 7 0.17500000000000002 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 7 0.17500000000000002 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 7 0.17500000000000002 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 7 0.17500000000000002 No Hit GGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTC 6 0.15 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 6 0.15 No Hit ATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCT 6 0.15 No Hit GAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAAT 6 0.15 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 6 0.15 No Hit ATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCC 6 0.15 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 6 0.15 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 6 0.15 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 6 0.15 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 6 0.15 No Hit TTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGG 6 0.15 No Hit TATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTA 6 0.15 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 6 0.15 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 5 0.125 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 5 0.125 No Hit CTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGT 5 0.125 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 5 0.125 No Hit TATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACA 5 0.125 No Hit GTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTG 5 0.125 No Hit AATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTAT 5 0.125 No Hit ATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATAT 5 0.125 No Hit CTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAG 5 0.125 No Hit TCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGA 5 0.125 No Hit CTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGC 5 0.125 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 5 0.125 No Hit CTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTT 5 0.125 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 5 0.125 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 5 0.125 No Hit TTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGG 5 0.125 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 5 0.125 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 5 0.125 No Hit TCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTG 5 0.125 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 5 0.125 No Hit GTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0125 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.037500000000000006 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.075 0.0 0.0 0.0 0.0 86-87 0.075 0.0 0.0 0.0 0.0 88-89 0.075 0.0 0.0 0.0 0.0 90-91 0.0875 0.0 0.0 0.0 0.0 92-93 0.1 0.0 0.0 0.0 0.0 94-95 0.125 0.0 0.0 0.0 0.0 96-97 0.125 0.0 0.0 0.0 0.0 98-99 0.125 0.0 0.0 0.0 0.0 100-101 0.15 0.0 0.0 0.0 0.0 102-103 0.15 0.0 0.0 0.0 0.0 104-105 0.15 0.0 0.0 0.0 0.0 106-107 0.15 0.0 0.0 0.0 0.0 108-109 0.16249999999999998 0.0 0.0 0.0 0.0 110-111 0.2 0.0 0.0 0.0 0.0 112-113 0.2375 0.0 0.0 0.0 0.0 114-115 0.3 0.0 0.0 0.0 0.0 116-117 0.3 0.0 0.0 0.0 0.0 118-119 0.375 0.0 0.0 0.0 0.0 120-121 0.4625 0.0 0.0 0.0 0.0 122-123 0.55 0.0 0.0 0.0 0.0 124-125 0.6 0.0 0.0 0.0 0.0 126-127 0.7124999999999999 0.0 0.0 0.0 0.0 128-129 0.8 0.0 0.0 0.0 0.0 130-131 0.8625 0.0 0.0 0.0 0.0 132-133 0.9 0.0 0.0 0.0 0.0 134-135 1.0125 0.0 0.0 0.0 0.0 136-137 1.15 0.0 0.0 0.0 0.0 138 1.175 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 3055546 spots for ERR11006596.sra Written 3055546 spots for ERR11006596.sra Read 3055545 spots for ERR11006596.sra Written 3055545 spots for ERR11006596.sra Read 3055545 spots for ERR11006596.sra Written 3055545 spots for ERR11006596.sra Read 3055545 spots for ERR11006596.sra Written 3055545 spots for ERR11006596.sra Read 3055545 spots for ERR11006596.sra Written 3055545 spots for ERR11006596.sra Read 3055545 spots for ERR11006596.sra Written 3055545 spots for ERR11006596.sra Read 3055545 spots for ERR11006596.sra Written 3055545 spots for ERR11006596.sra Read 3055545 spots for ERR11006596.sra Written 3055545 spots for ERR11006596.sra Read 3055545 spots for ERR11006596.sra Written 3055545 spots for ERR11006596.sra Read 3055545 spots for ERR11006596.sra Written 3055545 spots for ERR11006596.sra Read 3055545 spots for ERR11006596.sra Written 3055545 spots for ERR11006596.sra Read 3055545 spots for ERR11006596.sra Written 3055545 spots for ERR11006596.sra Read 3055545 spots for ERR11006596.sra Written 3055545 spots for ERR11006596.sra Read 3055545 spots for ERR11006596.sra Written 3055545 spots for ERR11006596.sra Read 3055545 spots for ERR11006596.sra Written 3055545 spots for ERR11006596.sra Read 3055545 spots for ERR11006596.sra Written 3055545 spots for ERR11006596.sra Read 3055545 spots for ERR11006596.sra Written 3055545 spots for ERR11006596.sra Read 3055545 spots for ERR11006596.sra Written 3055545 spots for ERR11006596.sra Read 3055545 spots for ERR11006596.sra Written 3055545 spots for ERR11006596.sra Read 3055545 spots for ERR11006596.sra Written 3055545 spots for ERR11006596.sra SRR ids: ['ERR11006596.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_61h6p44z ERR11006596.sra spots: 61110901 blocks: [[1, 3055545], [3055546, 6111090], [6111091, 9166635], [9166636, 12222180], [12222181, 15277725], [15277726, 18333270], [18333271, 21388815], [21388816, 24444360], [24444361, 27499905], [27499906, 30555450], [30555451, 33610995], [33610996, 36666540], [36666541, 39722085], [39722086, 42777630], [42777631, 45833175], [45833176, 48888720], [48888721, 51944265], [51944266, 54999810], [54999811, 58055355], [58055356, 61110901]] ERR11006596 file size 22456813 ERR11006596 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006596 ERR11006596_1.fastq ERR11006596_2.fastq Input file: ERR11006596_1.fastq Paired file: ERR11006596_2.fastq trimmed: ERR11006596-trimmed-pair1.fastq, ERR11006596-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 18:52:40 2024 >> started Fri Dec 6 18:54:42 2024 >> done (122.102s) 61110901 read pairs processed; of these: 679 ( 0.00%) short read pairs filtered out after trimming by size control 2079 ( 0.00%) empty read pairs filtered out after trimming by size control 61108143 (100.00%) read pairs available; of these: 1296589 ( 2.12%) trimmed read pairs available after processing 59811554 (97.88%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 48 0.00% 19 29 0.00% 20 33 0.00% 21 34 0.00% 22 42 0.00% 23 44 0.00% 24 48 0.00% 25 1668 0.00% 26 38 0.00% 27 34 0.00% 28 53 0.00% 29 53 0.00% 30 50 0.00% 31 110 0.00% 32 87 0.00% 33 51 0.00% 34 48 0.00% 35 68 0.00% 36 65 0.00% 37 55 0.00% 38 65 0.00% 39 75 0.00% 40 67 0.00% 41 85 0.00% 42 78 0.00% 43 99 0.00% 44 99 0.00% 45 92 0.00% 46 103 0.00% 47 121 0.00% 48 99 0.00% 49 112 0.00% 50 133 0.00% 51 150 0.00% 52 175 0.00% 53 195 0.00% 54 193 0.00% 55 282 0.00% 56 204 0.00% 57 214 0.00% 58 280 0.00% 59 251 0.00% 60 272 0.00% 61 307 0.00% 62 365 0.00% 63 390 0.00% 64 423 0.00% 65 448 0.00% 66 490 0.00% 67 503 0.00% 68 560 0.00% 69 573 0.00% 70 608 0.00% 71 684 0.00% 72 718 0.00% 73 874 0.00% 74 1006 0.00% 75 1040 0.00% 76 1183 0.00% 77 1232 0.00% 78 1321 0.00% 79 1345 0.00% 80 1491 0.00% 81 1534 0.00% 82 1732 0.00% 83 1927 0.00% 84 2118 0.00% 85 2364 0.00% 86 2653 0.00% 87 2821 0.00% 88 2858 0.00% 89 3105 0.01% 90 3266 0.01% 91 3433 0.01% 92 3404 0.01% 93 3757 0.01% 94 3960 0.01% 95 4370 0.01% 96 4745 0.01% 97 5147 0.01% 98 5566 0.01% 99 5699 0.01% 100 5874 0.01% 101 6293 0.01% 102 6540 0.01% 103 6827 0.01% 104 7217 0.01% 105 7805 0.01% 106 8674 0.01% 107 8962 0.01% 108 9191 0.02% 109 9842 0.02% 110 10349 0.02% 111 10709 0.02% 112 11771 0.02% 113 11820 0.02% 114 12334 0.02% 115 12895 0.02% 116 14612 0.02% 117 14651 0.02% 118 15432 0.03% 119 16826 0.03% 120 17399 0.03% 121 18877 0.03% 122 19236 0.03% 123 19727 0.03% 124 20964 0.03% 125 22413 0.04% 126 21693 0.04% 127 21823 0.04% 128 23296 0.04% 129 24933 0.04% 130 27282 0.04% 131 28480 0.05% 132 29292 0.05% 133 29162 0.05% 134 30140 0.05% 135 30895 0.05% 136 32212 0.05% 137 33130 0.05% 138 35157 0.06% 139 37342 0.06% 140 38849 0.06% 141 41332 0.07% 142 42459 0.07% 143 44012 0.07% 144 44513 0.07% 145 48225 0.08% 146 54099 0.09% 147 52340 0.09% 148 55187 0.09% 149 57374 0.09% 150 59811554 97.88% 61108143 reads passed initial QC criterion=sequence-density sequence-density=0.18 sequence-density-rank=1 fanout-score=1.00 fanout-score-rank=31 prefix-density=0.01 prefix-fanout=1.0 sequence=GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTTATCCTTCCACCGTTGGAAGCGGGCAGTTGTCGCTGCTCTGTGAAGCCAGCCTCACGCTGTGCCTGCCAACATTATGGGCCGCGAAGCCTAGCTTTCGCTTAAGCTCCAACGGCCCACTACGCAACTTGGAACGGGCGGGCCATCAGTAGCACACCCAGACCAGGCCCGCAGCGCTACGGCAACGTCCACACCACCCTTAAAGCCCCCAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=192.45 fanout-score-rank=1 prefix-density=0.14 prefix-fanout=10.9 sequence=AAAAAAAAGTATGTTTGCATCAATGATAATCCCAAAGTAGGAAGTGTGTACGTAGTAGCAAGTGTTATACATACATAAAATTAAGCGATGCGTTTCGATCAAGTACTTGGCATCATCGATCACATACGTACATTTCACAGCAGGTAGCTACGTACATTACAGGCATACGTACATGCAGAGAGATACCCGGCCCTGTGTTGTGTTTGCAATTGCATAGATGAGAATGAGATGACTTGATTCTTAATTAGCCGGCGAGAGCGCTGGTGTTGTAGACGAGGAGGGCGCCGCCGCCGAGGAGGCCGGCCAGGGTGACGGCCCAGATCAGGAGCCCCGTCGTCCCACCGGCGTAGCGGTCGCCAGATTCGGACCATACCTCCGGCGTGTAGATCGGGCTGTAGCCGTCGACGTTGGCGCCGTACTTGTCAACAAACTGGTACACACCCTTTCCCGTGCCCTTCCTGCCGTTGGCGTCGATGTCCACCGTCAAGCCACCTCCGATCCCGAGGGGCTT criterion=sequence-density sequence-density=0.20 sequence-density-rank=1 fanout-score=1.00 fanout-score-rank=38 prefix-density=0.02 prefix-fanout=1.0 sequence=GGGGGGGGGGATGTAAGAAAAATAACCAACTTGACCCTTAGCCCCGGTGTTATATTTGGTTATTTACTAAAATCCCCTTTTGGGGGAGAAGGCTGGATTGTTAGTGTGGATGATTTAGAAGATATAATTGGTGGACATGTATGGTTGGGTTTCATTTGTGTATTTGGCGGAATTTGGCATATCTTAACCAAACCTTTCGCATGGGCTCGCCGTGCATTTGTATGGTCTGGAGAAGCTTACTTGTCTTATAGTTTAGCTGCTTTATCTGTCTTTGGTTTTATCGCTTGTTGTTTTGTCTGGTTCAATAATACGGCTTATCCGAGTGAGTTTTATGGACCCACCGGCCCAGAAGCTTCTCAAGCTCAAGCATTTACTTTTCTAGTTAGAGACCAGCGTCTTGGAGCTAATGTGGGATCTGCTCAAGGACCCACAGGTTTAGGTAAATATCTAATGCGTTCCCCAACGGGAGAGGTTATCTTTGGAGGGGAAACTATGCGTTTTTGGGACCTCC criterion=fanout-score sequence-density=0.07 sequence-density-rank=23 fanout-score=82.33 fanout-score-rank=1 prefix-density=5.47 prefix-fanout=1.0 sequence=CTGGATAACTATCACTGAAAATC ERR11006596 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 18:55:21 Started mapping on | Dec 06 18:55:21 Finished on | Dec 06 19:02:40 Mapping speed, Million of reads per hour | 501.11 Number of input reads | 61108143 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 49382915 Uniquely mapped reads % | 80.81% Average mapped length | 297.88 Number of splices: Total | 23415613 Number of splices: Annotated (sjdb) | 21988787 Number of splices: GT/AG | 23044546 Number of splices: GC/AG | 259850 Number of splices: AT/AC | 12709 Number of splices: Non-canonical | 98508 Mismatch rate per base, % | 0.30% Deletion rate per base | 0.02% Deletion average length | 1.84 Insertion rate per base | 0.01% Insertion average length | 2.08 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 9263845 % of reads mapped to multiple loci | 15.16% Number of reads mapped to too many loci | 14133 % of reads mapped to too many loci | 0.02% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.95% % of reads unmapped: other | 1.05% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2461383 2461383 2461383 N_multimapping 9263845 9263845 9263845 N_noFeature 11841882 45518039 14696998 N_ambiguous 1487059 70548 436391 UnstrandedReadsAssigned:36053974 PositiveStrandReadsAssigned:3794328 NegativeStrandReadsAssigned:34249526 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006596 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006596-trimmed-pair1.fastq ERR11006596-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 61,108,143 reads, 38,337,253 reads pseudoaligned [quant] estimated average fragment length: 249.31 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,193 rounds 52973 ERR11006596.ke.tsv 35125 ERR11006596.se.tsv 88098 total ==> ERR11006596.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 687.938 0 0 PNS24247 1044 795.69 59.1873 2.25804 PNS24249 1928 1679.69 239.378 4.32615 PNS24246 1044 795.69 59.1873 2.25804 PNS24248 1044 795.69 59.1873 2.25804 PNS24244 1471 1222.69 181.06 4.49525 PNS24243 293 63.4117 0 0 KQK14069 1603 1354.69 678.409 15.2019 KQK14071 474 227.674 29.3456 3.9127 ==> ERR11006596.se.tsv <== BRADI_1g14170v3 863 BRADI_1g53295v3 1689 BRADI_1g59795v3 552 BRADI_1g07683v3 0 BRADI_1g00485v3 14 BRADI_1g20270v3 960 BRADI_1g74790v3 825 BRADI_1g09890v3 1 BRADI_1g77505v3 217 BRADI_1g48960v3 0 ERR11006596 completed mapping pipeline successfully