Starting /dee2/code/volunteer_pipeline.sh ERR11006597 current disk space = 1550008745984 free memory = 1330953660 ERR11006597 SRAfilesize a4aea26311b47dcbe575f03526b59b28 ERR11006597.sra ERR11006597.sra file validated ERR11006597 is paired end ERR11006597 is conventional basespace ERR11006597 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006597_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5875 37.0 37.0 37.0 37.0 37.0 2 36.3655 37.0 37.0 37.0 37.0 37.0 3 36.464 37.0 37.0 37.0 37.0 37.0 4 36.6385 37.0 37.0 37.0 37.0 37.0 5 36.5835 37.0 37.0 37.0 37.0 37.0 6 36.6365 37.0 37.0 37.0 37.0 37.0 7 36.5405 37.0 37.0 37.0 37.0 37.0 8 36.494 37.0 37.0 37.0 37.0 37.0 9 36.518 37.0 37.0 37.0 37.0 37.0 10-14 36.4812 37.0 37.0 37.0 37.0 37.0 15-19 36.5601 37.0 37.0 37.0 37.0 37.0 20-24 36.468900000000005 37.0 37.0 37.0 37.0 37.0 25-29 36.432399999999994 37.0 37.0 37.0 37.0 37.0 30-34 36.3556 37.0 37.0 37.0 37.0 37.0 35-39 36.383 37.0 37.0 37.0 37.0 37.0 40-44 36.3737 37.0 37.0 37.0 37.0 37.0 45-49 36.2869 37.0 37.0 37.0 37.0 37.0 50-54 36.2611 37.0 37.0 37.0 37.0 37.0 55-59 36.2839 37.0 37.0 37.0 37.0 37.0 60-64 36.17829999999999 37.0 37.0 37.0 37.0 37.0 65-69 36.1673 37.0 37.0 37.0 37.0 37.0 70-74 36.1287 37.0 37.0 37.0 37.0 37.0 75-79 36.154900000000005 37.0 37.0 37.0 37.0 37.0 80-84 36.1283 37.0 37.0 37.0 37.0 37.0 85-89 35.96640000000001 37.0 37.0 37.0 37.0 37.0 90-94 36.00079999999999 37.0 37.0 37.0 37.0 37.0 95-99 35.8969 37.0 37.0 37.0 37.0 37.0 100-104 35.9614 37.0 37.0 37.0 37.0 37.0 105-109 35.8586 37.0 37.0 37.0 37.0 37.0 110-114 35.8409 37.0 37.0 37.0 37.0 37.0 115-119 35.853899999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.75279999999999 37.0 37.0 37.0 37.0 37.0 125-129 35.665000000000006 37.0 37.0 37.0 37.0 37.0 130-134 35.6151 37.0 37.0 37.0 37.0 37.0 135-139 35.4431 37.0 37.0 37.0 37.0 37.0 140-144 35.361399999999996 37.0 37.0 37.0 34.6 37.0 145-149 35.431200000000004 37.0 37.0 37.0 37.0 37.0 150 35.3075 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 4.0 23 3.0 24 5.0 25 0.0 26 6.0 27 10.0 28 16.0 29 28.0 30 36.0 31 61.0 32 75.0 33 91.0 34 118.0 35 327.0 36 2943.0 37 277.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.175 13.425 15.85 35.55 2 25.575 11.1 30.225 33.1 3 22.825 13.925 27.875 35.375 4 25.6 18.175 25.124999999999996 31.1 5 28.075 23.175 23.925 24.825 6 23.525 30.25 22.125 24.099999999999998 7 17.575 28.7 35.575 18.15 8 17.075000000000003 29.275000000000002 32.925 20.724999999999998 9 18.325 25.974999999999998 34.525 21.175 10-14 20.395 31.330000000000002 26.525 21.75 15-19 20.65 31.385 25.415 22.55 20-24 20.19 28.765 27.800000000000004 23.244999999999997 25-29 21.895 29.715000000000003 26.384999999999998 22.005 30-34 22.935 30.12 25.2 21.745 35-39 22.125 30.580000000000002 24.945 22.35 40-44 20.29 29.880000000000003 26.619999999999997 23.21 45-49 20.96 29.01 26.935 23.095 50-54 20.885 30.635 26.150000000000002 22.33 55-59 22.21 29.395 24.779999999999998 23.615 60-64 21.09 30.375000000000004 25.174999999999997 23.36 65-69 21.59 29.48 26.06 22.869999999999997 70-74 22.18 29.794999999999998 24.005000000000003 24.02 75-79 21.990000000000002 29.375 25.5 23.135 80-84 22.735 29.015 25.095 23.155 85-89 23.365 28.525 24.9 23.21 90-94 21.81 30.009999999999998 25.27 22.91 95-99 22.650000000000002 28.735 24.765 23.849999999999998 100-104 21.85 30.0 24.465 23.685000000000002 105-109 22.445 28.615000000000002 25.35 23.59 110-114 22.994999999999997 28.294999999999998 25.224999999999998 23.485 115-119 21.235 30.59 24.87 23.305 120-124 21.395 29.659999999999997 24.415 24.529999999999998 125-129 21.72 30.214999999999996 23.275000000000002 24.79 130-134 22.814999999999998 30.205 24.52 22.46 135-139 23.275000000000002 28.860000000000003 24.37 23.494999999999997 140-144 23.419999999999998 29.7 25.15 21.73 145-149 22.86 29.93 24.4 22.81 150 21.0 27.55 28.1 23.35 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 0.0 20 0.0 21 1.0 22 2.5 23 2.5 24 3.0 25 4.5 26 4.5 27 8.5 28 17.5 29 20.5 30 22.0 31 26.5 32 36.5 33 49.0 34 59.0 35 68.0 36 95.5 37 164.5 38 239.5 39 239.0 40 246.0 41 275.5 42 236.5 43 213.5 44 207.0 45 199.5 46 196.0 47 170.0 48 141.0 49 109.5 50 86.0 51 68.5 52 55.5 53 44.5 54 36.0 55 39.5 56 34.0 57 27.0 58 28.5 59 32.0 60 32.0 61 31.5 62 35.5 63 38.0 64 44.0 65 48.0 66 34.0 67 24.0 68 26.5 69 25.0 70 23.5 71 24.5 72 21.5 73 16.5 74 16.0 75 14.5 76 9.5 77 5.5 78 3.5 79 3.5 80 2.5 81 1.0 82 1.0 83 0.5 84 0.5 85 1.5 86 1.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.275 #Duplication Level Percentage of deduplicated Percentage of total 1 83.7428475260855 62.2 2 10.972736452372938 16.3 3 2.1204981487714574 4.725 4 1.0097610232245036 3.0 5 0.6395153147088523 2.375 6 0.43756311006395154 1.95 7 0.26926960619320095 1.4000000000000001 8 0.23561090541905083 1.4000000000000001 9 0.03365870077415012 0.22499999999999998 >10 0.5048805116122518 4.775 >50 0.03365870077415012 1.6500000000000001 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 66 1.6500000000000001 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 18 0.44999999999999996 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 18 0.44999999999999996 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 18 0.44999999999999996 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 14 0.35000000000000003 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 13 0.325 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 13 0.325 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 13 0.325 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 12 0.3 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 12 0.3 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 10 0.25 No Hit CTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAG 10 0.25 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 10 0.25 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 10 0.25 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 10 0.25 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 10 0.25 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 9 0.22499999999999998 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 8 0.2 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 8 0.2 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 8 0.2 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 8 0.2 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 8 0.2 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 8 0.2 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 8 0.2 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 7 0.17500000000000002 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 7 0.17500000000000002 No Hit TCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGT 7 0.17500000000000002 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 7 0.17500000000000002 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 7 0.17500000000000002 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 7 0.17500000000000002 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 7 0.17500000000000002 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 7 0.17500000000000002 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 6 0.15 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 6 0.15 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 6 0.15 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 6 0.15 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 6 0.15 No Hit TAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCA 6 0.15 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 6 0.15 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 6 0.15 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 6 0.15 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 6 0.15 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 6 0.15 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 6 0.15 No Hit GGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGG 6 0.15 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 5 0.125 No Hit CTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAAT 5 0.125 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 5 0.125 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 5 0.125 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 5 0.125 No Hit GTCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 5 0.125 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 5 0.125 No Hit ACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAATA 5 0.125 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 5 0.125 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 5 0.125 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 5 0.125 No Hit CCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAA 5 0.125 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 5 0.125 No Hit TCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGT 5 0.125 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 5 0.125 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 5 0.125 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 5 0.125 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 5 0.125 No Hit TAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.1 0.0 0.0 0.0 0.0 82-83 0.1 0.0 0.0 0.0 0.0 84-85 0.1 0.0 0.0 0.0 0.0 86-87 0.1 0.0 0.0 0.0 0.0 88-89 0.1 0.0 0.0 0.0 0.0 90-91 0.1 0.0 0.0 0.0 0.0 92-93 0.1 0.0 0.0 0.0 0.0 94-95 0.1 0.0 0.0 0.0 0.0 96-97 0.1 0.0 0.0 0.0 0.0 98-99 0.125 0.0 0.0 0.0 0.0 100-101 0.175 0.0 0.0 0.0 0.0 102-103 0.1875 0.0 0.0 0.0 0.0 104-105 0.2 0.0 0.0 0.0 0.0 106-107 0.275 0.0 0.0 0.0 0.0 108-109 0.3125 0.0 0.0 0.0 0.0 110-111 0.3625 0.0 0.0 0.0 0.0 112-113 0.4 0.0 0.0 0.0 0.0 114-115 0.44999999999999996 0.0 0.0 0.0 0.0 116-117 0.5625 0.0 0.0 0.0 0.0 118-119 0.6875 0.0 0.0 0.0 0.0 120-121 0.8 0.0 0.0 0.0 0.0 122-123 0.8625 0.0 0.0 0.0 0.0 124-125 0.975 0.0 0.0 0.0 0.0 126-127 1.1 0.0 0.0 0.0 0.0 128-129 1.2125 0.0 0.0 0.0 0.0 130-131 1.2999999999999998 0.0 0.0 0.0 0.0 132-133 1.3875 0.0 0.0 0.0 0.0 134-135 1.5750000000000002 0.0 0.0 0.0 0.0 136-137 1.6625 0.0 0.0 0.0 0.0 138 1.775 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE ERR11006597 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006597_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.037 37.0 37.0 37.0 37.0 37.0 2 36.151 37.0 37.0 37.0 37.0 37.0 3 36.3555 37.0 37.0 37.0 37.0 37.0 4 36.344 37.0 37.0 37.0 37.0 37.0 5 36.364 37.0 37.0 37.0 37.0 37.0 6 36.282 37.0 37.0 37.0 37.0 37.0 7 36.3 37.0 37.0 37.0 37.0 37.0 8 36.406 37.0 37.0 37.0 37.0 37.0 9 36.2625 37.0 37.0 37.0 37.0 37.0 10-14 36.360699999999994 37.0 37.0 37.0 37.0 37.0 15-19 36.290499999999994 37.0 37.0 37.0 37.0 37.0 20-24 36.3284 37.0 37.0 37.0 37.0 37.0 25-29 36.21990000000001 37.0 37.0 37.0 37.0 37.0 30-34 36.1881 37.0 37.0 37.0 37.0 37.0 35-39 36.1074 37.0 37.0 37.0 37.0 37.0 40-44 36.1795 37.0 37.0 37.0 37.0 37.0 45-49 36.1186 37.0 37.0 37.0 37.0 37.0 50-54 36.01219999999999 37.0 37.0 37.0 37.0 37.0 55-59 35.926700000000004 37.0 37.0 37.0 37.0 37.0 60-64 35.9483 37.0 37.0 37.0 37.0 37.0 65-69 35.888 37.0 37.0 37.0 37.0 37.0 70-74 35.8597 37.0 37.0 37.0 37.0 37.0 75-79 35.69969999999999 37.0 37.0 37.0 37.0 37.0 80-84 35.7423 37.0 37.0 37.0 37.0 37.0 85-89 35.751400000000004 37.0 37.0 37.0 37.0 37.0 90-94 35.7305 37.0 37.0 37.0 37.0 37.0 95-99 35.6767 37.0 37.0 37.0 37.0 37.0 100-104 35.5899 37.0 37.0 37.0 37.0 37.0 105-109 35.511900000000004 37.0 37.0 37.0 37.0 37.0 110-114 35.5712 37.0 37.0 37.0 37.0 37.0 115-119 35.3361 37.0 37.0 37.0 34.6 37.0 120-124 35.2801 37.0 37.0 37.0 27.4 37.0 125-129 35.1303 37.0 37.0 37.0 25.0 37.0 130-134 34.9956 37.0 37.0 37.0 25.0 37.0 135-139 35.0803 37.0 37.0 37.0 25.0 37.0 140-144 34.861599999999996 37.0 37.0 37.0 25.0 37.0 145-149 34.739599999999996 37.0 37.0 37.0 25.0 37.0 150 34.69 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 1.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 5.0 23 8.0 24 6.0 25 5.0 26 11.0 27 11.0 28 14.0 29 28.0 30 32.0 31 57.0 32 61.0 33 145.0 34 256.0 35 709.0 36 2515.0 37 135.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.775000000000002 24.55 17.275 29.4 2 24.675 27.025 33.4 14.899999999999999 3 21.075 29.175 30.525000000000002 19.225 4 23.45 30.975 24.625 20.95 5 24.85 33.300000000000004 24.625 17.224999999999998 6 20.875 35.975 24.55 18.6 7 18.025 19.575 41.375 21.025 8 22.0 23.775 30.55 23.674999999999997 9 23.7 20.549999999999997 32.975 22.775000000000002 10-14 23.064999999999998 26.915 28.035 21.985 15-19 23.93 25.845000000000002 28.875 21.349999999999998 20-24 24.035 25.674999999999997 28.98 21.310000000000002 25-29 23.630000000000003 25.840000000000003 29.160000000000004 21.37 30-34 23.71 25.6 29.43 21.26 35-39 24.265 25.22 28.999999999999996 21.515 40-44 23.14 26.05 29.215000000000003 21.595 45-49 23.89 26.93 27.905 21.275 50-54 24.305 25.474999999999998 28.439999999999998 21.78 55-59 23.215 25.465 28.895 22.425 60-64 22.939999999999998 25.424999999999997 29.385 22.25 65-69 24.085 25.52 28.615000000000002 21.78 70-74 23.724999999999998 25.895000000000003 29.24 21.14 75-79 23.674999999999997 25.564999999999998 29.349999999999998 21.41 80-84 23.84 24.990000000000002 29.330000000000002 21.84 85-89 24.759999999999998 25.115 28.244999999999997 21.88 90-94 23.74 25.005 29.360000000000003 21.895 95-99 23.974999999999998 24.815 29.04 22.17 100-104 23.724999999999998 24.215 30.490000000000002 21.57 105-109 25.169999999999998 24.585 28.62 21.625 110-114 23.36 25.230000000000004 29.085 22.325 115-119 23.365 25.405 28.74 22.49 120-124 24.01 25.52 28.58 21.89 125-129 23.02 25.46 29.145 22.375 130-134 23.055 26.055 29.015 21.875 135-139 24.22 25.41 29.75 20.62 140-144 23.655 25.545 29.28 21.52 145-149 23.380000000000003 25.230000000000004 29.175 22.215 150 22.85 23.35 30.225 23.575 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 1.0 2 1.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 3.0 20 2.5 21 1.0 22 2.5 23 5.5 24 8.5 25 9.0 26 10.0 27 10.0 28 15.0 29 24.0 30 35.0 31 42.0 32 46.0 33 51.0 34 56.5 35 70.0 36 104.5 37 175.0 38 210.0 39 191.0 40 206.0 41 228.0 42 212.5 43 220.0 44 234.0 45 221.5 46 197.0 47 164.5 48 138.0 49 108.0 50 81.0 51 63.5 52 51.5 53 54.0 54 44.5 55 32.0 56 41.5 57 40.5 58 32.5 59 32.0 60 37.0 61 39.5 62 37.0 63 46.0 64 47.0 65 43.5 66 44.5 67 28.5 68 19.5 69 26.0 70 31.0 71 28.5 72 18.5 73 14.0 74 14.5 75 11.5 76 7.5 77 8.0 78 7.0 79 4.5 80 3.5 81 2.5 82 1.0 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.44999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 83.13452617627568 62.724999999999994 2 11.530815109343937 17.4 3 2.1537442014579193 4.875 4 1.2591119946984757 3.8 5 0.4970178926441352 1.875 6 0.36447978793903246 1.6500000000000001 7 0.26507620941020543 1.4000000000000001 8 0.26507620941020543 1.6 9 0.23194168323392977 1.575 >10 0.2982107355864811 3.1 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 25 0.625 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 21 0.525 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 14 0.35000000000000003 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 13 0.325 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 11 0.27499999999999997 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 10 0.25 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 10 0.25 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 10 0.25 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 10 0.25 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 9 0.22499999999999998 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 9 0.22499999999999998 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 9 0.22499999999999998 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 9 0.22499999999999998 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 9 0.22499999999999998 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 9 0.22499999999999998 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 9 0.22499999999999998 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 8 0.2 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 8 0.2 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 8 0.2 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 8 0.2 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 8 0.2 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 8 0.2 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 8 0.2 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 8 0.2 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 7 0.17500000000000002 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 7 0.17500000000000002 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 7 0.17500000000000002 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 7 0.17500000000000002 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 7 0.17500000000000002 No Hit CTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAG 7 0.17500000000000002 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 7 0.17500000000000002 No Hit GAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGT 7 0.17500000000000002 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 6 0.15 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 6 0.15 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 6 0.15 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 6 0.15 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 6 0.15 No Hit GGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTT 6 0.15 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 6 0.15 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 6 0.15 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 6 0.15 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 6 0.15 No Hit GTGGACGTTGCCGTAGCGCTGCGGGCCTGGTCTGGGTGTGCTACTGATGG 6 0.15 No Hit GGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTC 5 0.125 No Hit GTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTCA 5 0.125 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 5 0.125 No Hit ATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTG 5 0.125 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 5 0.125 No Hit CTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAG 5 0.125 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 5 0.125 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 5 0.125 No Hit CTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGG 5 0.125 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 5 0.125 No Hit TAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAG 5 0.125 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 5 0.125 No Hit AATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTA 5 0.125 No Hit GTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAAT 5 0.125 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.1 0.0 0.0 0.0 0.0 82-83 0.1 0.0 0.0 0.0 0.0 84-85 0.1 0.0 0.0 0.0 0.0 86-87 0.1 0.0 0.0 0.0 0.0 88-89 0.1 0.0 0.0 0.0 0.0 90-91 0.1 0.0 0.0 0.0 0.0 92-93 0.1 0.0 0.0 0.0 0.0 94-95 0.1 0.0 0.0 0.0 0.0 96-97 0.1125 0.0 0.0 0.0 0.0 98-99 0.16249999999999998 0.0 0.0 0.0 0.0 100-101 0.225 0.0 0.0 0.0 0.0 102-103 0.2375 0.0 0.0 0.0 0.0 104-105 0.25 0.0 0.0 0.0 0.0 106-107 0.325 0.0 0.0 0.0 0.0 108-109 0.3875 0.0 0.0 0.0 0.0 110-111 0.4375 0.0 0.0 0.0 0.0 112-113 0.475 0.0 0.0 0.0 0.0 114-115 0.525 0.0 0.0 0.0 0.0 116-117 0.6375 0.0 0.0 0.0 0.0 118-119 0.7625 0.0 0.0 0.0 0.0 120-121 0.875 0.0 0.0 0.0 0.0 122-123 0.9375 0.0 0.0 0.0 0.0 124-125 1.075 0.0 0.0 0.0 0.0 126-127 1.1749999999999998 0.0 0.0 0.0 0.0 128-129 1.2875 0.0 0.0 0.0 0.0 130-131 1.375 0.0 0.0 0.0 0.0 132-133 1.4625 0.0 0.0 0.0 0.0 134-135 1.65 0.0 0.0 0.0 0.0 136-137 1.7374999999999998 0.0 0.0 0.0 0.0 138 1.85 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGTGT 10 0.006973645 144.0 6 ATGTGTG 10 0.006973645 144.0 7 ATATGTG 10 0.006973645 144.0 5 TAATATG 10 0.006973645 144.0 3 AATATGT 10 0.006973645 144.0 4 TTAATAT 10 0.006973645 144.0 2 TGTGTGC 10 0.006973645 144.0 8 GTTAATA 10 0.006973645 144.0 1 >>END_MODULE Read 2524321 spots for ERR11006597.sra Written 2524321 spots for ERR11006597.sra Read 2524321 spots for ERR11006597.sra Written 2524321 spots for ERR11006597.sra Read 2524321 spots for ERR11006597.sra Written 2524321 spots for ERR11006597.sra Read 2524321 spots for ERR11006597.sra Written 2524321 spots for ERR11006597.sra Read 2524321 spots for ERR11006597.sra Written 2524321 spots for ERR11006597.sra Read 2524321 spots for ERR11006597.sra Written 2524321 spots for ERR11006597.sra Read 2524321 spots for ERR11006597.sra Written 2524321 spots for ERR11006597.sra Read 2524321 spots for ERR11006597.sra Written 2524321 spots for ERR11006597.sra Read 2524321 spots for ERR11006597.sra Written 2524321 spots for ERR11006597.sra Read 2524321 spots for ERR11006597.sra Written 2524321 spots for ERR11006597.sra Read 2524321 spots for ERR11006597.sra Written 2524321 spots for ERR11006597.sra Read 2524321 spots for ERR11006597.sra Written 2524321 spots for ERR11006597.sra Read 2524321 spots for ERR11006597.sra Written 2524321 spots for ERR11006597.sra Read 2524321 spots for ERR11006597.sra Written 2524321 spots for ERR11006597.sra Read 2524321 spots for ERR11006597.sra Written 2524321 spots for ERR11006597.sra Read 2524321 spots for ERR11006597.sra Written 2524321 spots for ERR11006597.sra Read 2524321 spots for ERR11006597.sra Written 2524321 spots for ERR11006597.sra Read 2524333 spots for ERR11006597.sra Written 2524333 spots for ERR11006597.sra Read 2524321 spots for ERR11006597.sra Written 2524321 spots for ERR11006597.sra Read 2524321 spots for ERR11006597.sra Written 2524321 spots for ERR11006597.sra SRR ids: ['ERR11006597.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_1cpfk44c ERR11006597.sra spots: 50486432 blocks: [[1, 2524321], [2524322, 5048642], [5048643, 7572963], [7572964, 10097284], [10097285, 12621605], [12621606, 15145926], [15145927, 17670247], [17670248, 20194568], [20194569, 22718889], [22718890, 25243210], [25243211, 27767531], [27767532, 30291852], [30291853, 32816173], [32816174, 35340494], [35340495, 37864815], [37864816, 40389136], [40389137, 42913457], [42913458, 45437778], [45437779, 47962099], [47962100, 50486432]] ERR11006597 file size 18550665 ERR11006597 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006597 ERR11006597_1.fastq ERR11006597_2.fastq Input file: ERR11006597_1.fastq Paired file: ERR11006597_2.fastq trimmed: ERR11006597-trimmed-pair1.fastq, ERR11006597-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 19:04:49 2024 >> started Fri Dec 6 19:05:49 2024 >> done (59.979s) 50486432 read pairs processed; of these: 913 ( 0.00%) short read pairs filtered out after trimming by size control 2169 ( 0.00%) empty read pairs filtered out after trimming by size control 50483350 (99.99%) read pairs available; of these: 1328512 ( 2.63%) trimmed read pairs available after processing 49154838 (97.37%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 39 0.00% 19 40 0.00% 20 34 0.00% 21 54 0.00% 22 64 0.00% 23 42 0.00% 24 29 0.00% 25 1224 0.00% 26 21 0.00% 27 38 0.00% 28 41 0.00% 29 49 0.00% 30 40 0.00% 31 94 0.00% 32 51 0.00% 33 37 0.00% 34 35 0.00% 35 44 0.00% 36 35 0.00% 37 55 0.00% 38 51 0.00% 39 64 0.00% 40 48 0.00% 41 62 0.00% 42 68 0.00% 43 82 0.00% 44 75 0.00% 45 71 0.00% 46 88 0.00% 47 83 0.00% 48 86 0.00% 49 129 0.00% 50 106 0.00% 51 108 0.00% 52 139 0.00% 53 144 0.00% 54 174 0.00% 55 213 0.00% 56 183 0.00% 57 198 0.00% 58 245 0.00% 59 237 0.00% 60 295 0.00% 61 271 0.00% 62 335 0.00% 63 397 0.00% 64 380 0.00% 65 471 0.00% 66 509 0.00% 67 508 0.00% 68 616 0.00% 69 609 0.00% 70 604 0.00% 71 707 0.00% 72 807 0.00% 73 890 0.00% 74 973 0.00% 75 1157 0.00% 76 1253 0.00% 77 1345 0.00% 78 1413 0.00% 79 1439 0.00% 80 1631 0.00% 81 1698 0.00% 82 1962 0.00% 83 2114 0.00% 84 2230 0.00% 85 2635 0.01% 86 2978 0.01% 87 3074 0.01% 88 3416 0.01% 89 3533 0.01% 90 3655 0.01% 91 3926 0.01% 92 3980 0.01% 93 4248 0.01% 94 4539 0.01% 95 4982 0.01% 96 5410 0.01% 97 5967 0.01% 98 6280 0.01% 99 6369 0.01% 100 6711 0.01% 101 6991 0.01% 102 7204 0.01% 103 7406 0.01% 104 7950 0.02% 105 8678 0.02% 106 9274 0.02% 107 9918 0.02% 108 10282 0.02% 109 10807 0.02% 110 11328 0.02% 111 11766 0.02% 112 12613 0.02% 113 12637 0.03% 114 13287 0.03% 115 14139 0.03% 116 15691 0.03% 117 16025 0.03% 118 16433 0.03% 119 18030 0.04% 120 18789 0.04% 121 19738 0.04% 122 20594 0.04% 123 20816 0.04% 124 22272 0.04% 125 23478 0.05% 126 22614 0.04% 127 23013 0.05% 128 24905 0.05% 129 26498 0.05% 130 28439 0.06% 131 29456 0.06% 132 30425 0.06% 133 29424 0.06% 134 30831 0.06% 135 30560 0.06% 136 32473 0.06% 137 33042 0.07% 138 35347 0.07% 139 37279 0.07% 140 38713 0.08% 141 41007 0.08% 142 42101 0.08% 143 42773 0.08% 144 43858 0.09% 145 46946 0.09% 146 51777 0.10% 147 50978 0.10% 148 53411 0.11% 149 55459 0.11% 150 49154838 97.37% 50483350 reads passed initial QC criterion=sequence-density sequence-density=0.21 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=21 prefix-density=0.19 prefix-fanout=2.0 sequence=TCTAATTCAAAA criterion=fanout-score sequence-density=0.01 sequence-density-rank=29 fanout-score=30.95 fanout-score-rank=1 prefix-density=0.25 prefix-fanout=1.1 sequence=CTTTTTTCACATCTGTAACTACATTCTCACATTTCTTTTTTGATCTCATGACTTTTTATGCGATCAGAAAACGAATAAGATCAAAAAGGCCATCATTAGGGCAAACAATGCAATAGCTTCGGTGAGAGCAAAGCCCAAAATGGCATAACCAAATAATTGTTTAGCCAATGATGGATTTCGCGCCACGGAATGAATCAAAGAACTGAAAACGTTTCCAATACCGACAGCAGCTCCGG criterion=sequence-density sequence-density=0.19 sequence-density-rank=1 fanout-score=1.00 fanout-score-rank=41 prefix-density=0.02 prefix-fanout=1.0 sequence=GGGGGGGGGGATGTAAGAAAAATAACCAACTTGACCCTTAGCCCCGGTGTTATATTTGGTTATTTACTAAAATCCCCTTTTGGGGGAGAAGGCTGGATTGTTAGTGTGGATGATTTAGAAGATATAATTGGTGGACATGTATGGTTGGGTTTCATTTGTGTATTTGGCGGAATTTGGCATATCTTAACCAAACCTTTCGCATGGGCTCGCCGTGCATTTGTATGGTCTGGAGAAGCTTACTTGTCTTATAGTTTAGCTGCTTTATCTGTCTTTGGTTTTATCGCTTGTTGTTTTGTCTGGTTCAATAATACGGCTTATCCGAGTGAGTTTTATGGACCCACCGGCCCAGAAGCTTCTCAAGCTCAAGCATTTACTTTTCTAGTTAGAGACCAGCGTCTTGGAGCTAATGTGGGATCTGCTCAAGGACCCACAGGTTTAGGTAAATATCTAATGCGTTCCCCAACGGGAGAGGTTATCTTTGGAGGGGAAACTATGCGTTTTTGGGACCTCC criterion=fanout-score sequence-density=0.08 sequence-density-rank=19 fanout-score=77.54 fanout-score-rank=1 prefix-density=6.07 prefix-fanout=1.0 sequence=ATAACTAGCACAGAAAATCGTCT ERR11006597 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 19:07:22 Started mapping on | Dec 06 19:07:22 Finished on | Dec 06 19:13:11 Mapping speed, Million of reads per hour | 520.75 Number of input reads | 50483350 Average input read length | 298 UNIQUE READS: Uniquely mapped reads number | 39153154 Uniquely mapped reads % | 77.56% Average mapped length | 297.67 Number of splices: Total | 18451398 Number of splices: Annotated (sjdb) | 17350314 Number of splices: GT/AG | 18126724 Number of splices: GC/AG | 212214 Number of splices: AT/AC | 19570 Number of splices: Non-canonical | 92890 Mismatch rate per base, % | 0.31% Deletion rate per base | 0.02% Deletion average length | 1.87 Insertion rate per base | 0.01% Insertion average length | 2.09 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 9309054 % of reads mapped to multiple loci | 18.44% Number of reads mapped to too many loci | 11988 % of reads mapped to too many loci | 0.02% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.06% % of reads unmapped: other | 0.92% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2021142 2021142 2021142 N_multimapping 9309054 9309054 9309054 N_noFeature 9296163 36369367 11227124 N_ambiguous 1343834 57240 467946 UnstrandedReadsAssigned:28513157 PositiveStrandReadsAssigned:2726547 NegativeStrandReadsAssigned:27458084 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006597 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006597-trimmed-pair1.fastq ERR11006597-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 50,483,350 reads, 32,067,142 reads pseudoaligned [quant] estimated average fragment length: 246.688 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,270 rounds 52973 ERR11006597.ke.tsv 35125 ERR11006597.se.tsv 88098 total ==> ERR11006597.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 690.566 0 0 PNS24247 1044 798.312 36.2419 1.59678 PNS24249 1928 1682.31 149.882 3.13364 PNS24246 1044 798.312 36.2419 1.59678 PNS24248 1044 798.312 36.2419 1.59678 PNS24244 1471 1225.31 103.392 2.96789 PNS24243 293 65.586 0 0 KQK14069 1603 1357.31 510.642 13.2325 KQK14071 474 230.171 40.4775 6.18543 ==> ERR11006597.se.tsv <== BRADI_1g14170v3 681 BRADI_1g53295v3 1060 BRADI_1g59795v3 432 BRADI_1g07683v3 0 BRADI_1g00485v3 11 BRADI_1g20270v3 602 BRADI_1g74790v3 442 BRADI_1g09890v3 0 BRADI_1g77505v3 171 BRADI_1g48960v3 0 ERR11006597 completed mapping pipeline successfully