Starting /dee2/code/volunteer_pipeline.sh ERR11006598 current disk space = 1550037663744 free memory = 1346050588 ERR11006598 SRAfilesize ccdd81c3ab5bc04c7dd0089606cc8f28 ERR11006598.sra ERR11006598.sra file validated ERR11006598 is paired end ERR11006598 is conventional basespace ERR11006598 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006598_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.247 37.0 37.0 37.0 37.0 37.0 2 36.2015 37.0 37.0 37.0 37.0 37.0 3 36.325 37.0 37.0 37.0 37.0 37.0 4 36.4145 37.0 37.0 37.0 37.0 37.0 5 36.4265 37.0 37.0 37.0 37.0 37.0 6 36.4095 37.0 37.0 37.0 37.0 37.0 7 36.4355 37.0 37.0 37.0 37.0 37.0 8 36.3445 37.0 37.0 37.0 37.0 37.0 9 36.396 37.0 37.0 37.0 37.0 37.0 10-14 36.426300000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.42289999999999 37.0 37.0 37.0 37.0 37.0 20-24 36.3937 37.0 37.0 37.0 37.0 37.0 25-29 36.33030000000001 37.0 37.0 37.0 37.0 37.0 30-34 36.3178 37.0 37.0 37.0 37.0 37.0 35-39 36.2443 37.0 37.0 37.0 37.0 37.0 40-44 36.2142 37.0 37.0 37.0 37.0 37.0 45-49 36.2279 37.0 37.0 37.0 37.0 37.0 50-54 36.212599999999995 37.0 37.0 37.0 37.0 37.0 55-59 36.1986 37.0 37.0 37.0 37.0 37.0 60-64 36.1154 37.0 37.0 37.0 37.0 37.0 65-69 36.1541 37.0 37.0 37.0 37.0 37.0 70-74 36.1156 37.0 37.0 37.0 37.0 37.0 75-79 35.9781 37.0 37.0 37.0 37.0 37.0 80-84 36.0604 37.0 37.0 37.0 37.0 37.0 85-89 36.0072 37.0 37.0 37.0 37.0 37.0 90-94 35.9485 37.0 37.0 37.0 37.0 37.0 95-99 36.0184 37.0 37.0 37.0 37.0 37.0 100-104 35.998599999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.85080000000001 37.0 37.0 37.0 37.0 37.0 110-114 35.7596 37.0 37.0 37.0 37.0 37.0 115-119 35.7228 37.0 37.0 37.0 37.0 37.0 120-124 35.737700000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.661199999999994 37.0 37.0 37.0 37.0 37.0 130-134 35.5882 37.0 37.0 37.0 37.0 37.0 135-139 35.503299999999996 37.0 37.0 37.0 37.0 37.0 140-144 35.3488 37.0 37.0 37.0 37.0 37.0 145-149 35.493100000000005 37.0 37.0 37.0 37.0 37.0 150 35.5545 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 1.0 19 0.0 20 1.0 21 1.0 22 0.0 23 2.0 24 1.0 25 6.0 26 10.0 27 14.0 28 17.0 29 28.0 30 51.0 31 57.0 32 78.0 33 113.0 34 165.0 35 296.0 36 2789.0 37 369.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.375 12.7 16.75 35.175 2 29.375 11.825 26.825 31.974999999999998 3 22.3 13.600000000000001 25.8 38.3 4 27.700000000000003 15.950000000000001 22.85 33.5 5 30.45 20.075000000000003 24.099999999999998 25.374999999999996 6 26.424999999999997 27.224999999999998 20.8 25.55 7 18.099999999999998 29.9 32.25 19.75 8 20.325 28.475 30.575000000000003 20.625 9 18.95 27.125 34.025 19.900000000000002 10-14 21.745 30.975 25.745 21.535 15-19 21.955 29.455 24.895 23.695 20-24 21.2 28.060000000000002 25.96 24.779999999999998 25-29 22.965 29.115000000000002 24.37 23.549999999999997 30-34 23.544999999999998 29.020000000000003 24.275 23.16 35-39 23.77 29.330000000000002 23.805 23.095 40-44 22.545 28.935 24.8 23.72 45-49 22.895 27.834999999999997 25.14 24.13 50-54 22.28 29.455 25.085 23.18 55-59 22.905 27.625 23.685000000000002 25.785000000000004 60-64 21.3 28.92 25.535000000000004 24.245 65-69 22.785 28.199999999999996 24.585 24.43 70-74 23.93 27.915 23.02 25.135 75-79 23.04 28.46 24.575 23.925 80-84 24.240000000000002 27.735 23.755000000000003 24.27 85-89 23.580000000000002 27.0 24.67 24.75 90-94 22.145 27.725 24.965 25.165 95-99 24.349999999999998 27.925 23.5 24.224999999999998 100-104 23.494999999999997 28.51 23.025000000000002 24.97 105-109 22.89 27.794999999999998 24.13 25.185000000000002 110-114 23.465 26.85 24.365000000000002 25.319999999999997 115-119 22.13 28.555000000000003 23.95 25.365 120-124 22.32 28.000000000000004 24.57 25.11 125-129 21.83 29.049999999999997 23.655 25.465 130-134 23.849999999999998 28.48 23.205000000000002 24.465 135-139 23.94 28.58 22.884999999999998 24.595 140-144 24.265 27.605 25.009999999999998 23.119999999999997 145-149 23.535 28.194999999999997 23.86 24.41 150 24.099999999999998 26.674999999999997 25.424999999999997 23.799999999999997 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 0.5 22 0.0 23 1.0 24 1.0 25 0.5 26 1.0 27 2.0 28 3.5 29 9.0 30 11.5 31 14.5 32 22.5 33 32.5 34 46.0 35 52.5 36 77.5 37 135.0 38 192.5 39 205.5 40 212.0 41 244.0 42 232.5 43 220.5 44 209.0 45 187.5 46 185.5 47 168.5 48 146.5 49 118.5 50 90.0 51 76.0 52 67.0 53 52.0 54 42.5 55 45.5 56 50.0 57 47.0 58 37.0 59 31.5 60 44.0 61 49.5 62 43.5 63 43.0 64 71.0 65 94.5 66 68.0 67 38.5 68 34.0 69 33.5 70 31.5 71 36.0 72 33.5 73 21.5 74 14.0 75 15.0 76 14.5 77 13.0 78 10.0 79 5.0 80 4.5 81 3.5 82 1.0 83 1.5 84 1.5 85 1.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.275 #Duplication Level Percentage of deduplicated Percentage of total 1 84.22451012952507 63.4 2 11.092660245765526 16.7 3 1.8598472268349386 4.2 4 1.195616074393889 3.5999999999999996 5 0.3653271338425772 1.375 6 0.4317502490866822 1.95 7 0.09963467286615742 0.525 8 0.1328462304882099 0.8 9 0.033211557622052475 0.22499999999999998 >10 0.5313849219528396 5.875 >50 0.033211557622052475 1.35 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 54 1.35 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 28 0.7000000000000001 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 21 0.525 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 20 0.5 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 18 0.44999999999999996 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 17 0.42500000000000004 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 16 0.4 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 15 0.375 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 14 0.35000000000000003 No Hit TAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGA 13 0.325 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 11 0.27499999999999997 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 11 0.27499999999999997 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 11 0.27499999999999997 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 10 0.25 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 10 0.25 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 10 0.25 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 10 0.25 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 9 0.22499999999999998 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 8 0.2 No Hit CACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCA 8 0.2 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 8 0.2 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 8 0.2 No Hit CCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTT 7 0.17500000000000002 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 7 0.17500000000000002 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 7 0.17500000000000002 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 6 0.15 No Hit GCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAA 6 0.15 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 6 0.15 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 6 0.15 No Hit GCAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAA 6 0.15 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 6 0.15 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 6 0.15 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 6 0.15 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 6 0.15 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 6 0.15 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 6 0.15 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 6 0.15 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 6 0.15 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 5 0.125 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 5 0.125 No Hit TCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGT 5 0.125 No Hit GTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCG 5 0.125 No Hit TTCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCT 5 0.125 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 5 0.125 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 5 0.125 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 5 0.125 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 5 0.125 No Hit CACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAAT 5 0.125 No Hit GCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.037500000000000006 0.0 0.0 0.0 0.0 90-91 0.05 0.0 0.0 0.0 0.0 92-93 0.075 0.0 0.0 0.0 0.0 94-95 0.075 0.0 0.0 0.0 0.0 96-97 0.075 0.0 0.0 0.0 0.0 98-99 0.075 0.0 0.0 0.0 0.0 100-101 0.0875 0.0 0.0 0.0 0.0 102-103 0.1 0.0 0.0 0.0 0.0 104-105 0.1125 0.0 0.0 0.0 0.0 106-107 0.16249999999999998 0.0 0.0 0.0 0.0 108-109 0.175 0.0 0.0 0.0 0.0 110-111 0.2 0.0 0.0 0.0 0.0 112-113 0.2375 0.0 0.0 0.0 0.0 114-115 0.35 0.0 0.0 0.0 0.0 116-117 0.44999999999999996 0.0 0.0 0.0 0.0 118-119 0.5375 0.0 0.0 0.0 0.0 120-121 0.6 0.0 0.0 0.0 0.0 122-123 0.6 0.0 0.0 0.0 0.0 124-125 0.625 0.0 0.0 0.0 0.0 126-127 0.6625000000000001 0.0 0.0 0.0 0.0 128-129 0.7375 0.0 0.0 0.0 0.0 130-131 0.8125 0.0 0.0 0.0 0.0 132-133 0.9 0.0 0.0 0.0 0.0 134-135 1.0125 0.0 0.0 0.0 0.0 136-137 1.0625 0.0 0.0 0.0 0.0 138 1.15 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCTAGT 10 0.006973645 144.0 4 AGTGTGA 10 0.006973645 144.0 8 CTAGTGT 10 0.006973645 144.0 6 CCTAGTG 10 0.006973645 144.0 5 GTGTGAG 10 0.006973645 144.0 9 >>END_MODULE ERR11006598 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006598_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.7115 37.0 37.0 37.0 37.0 37.0 2 36.1885 37.0 37.0 37.0 37.0 37.0 3 36.297 37.0 37.0 37.0 37.0 37.0 4 36.3215 37.0 37.0 37.0 37.0 37.0 5 36.424 37.0 37.0 37.0 37.0 37.0 6 36.3215 37.0 37.0 37.0 37.0 37.0 7 36.3125 37.0 37.0 37.0 37.0 37.0 8 36.3005 37.0 37.0 37.0 37.0 37.0 9 36.317 37.0 37.0 37.0 37.0 37.0 10-14 36.314499999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.2537 37.0 37.0 37.0 37.0 37.0 20-24 36.2614 37.0 37.0 37.0 37.0 37.0 25-29 36.2086 37.0 37.0 37.0 37.0 37.0 30-34 36.2338 37.0 37.0 37.0 37.0 37.0 35-39 36.1563 37.0 37.0 37.0 37.0 37.0 40-44 36.1808 37.0 37.0 37.0 37.0 37.0 45-49 36.1371 37.0 37.0 37.0 37.0 37.0 50-54 36.08970000000001 37.0 37.0 37.0 37.0 37.0 55-59 36.0384 37.0 37.0 37.0 37.0 37.0 60-64 36.0077 37.0 37.0 37.0 37.0 37.0 65-69 36.013799999999996 37.0 37.0 37.0 37.0 37.0 70-74 35.9611 37.0 37.0 37.0 37.0 37.0 75-79 35.9093 37.0 37.0 37.0 37.0 37.0 80-84 35.854099999999995 37.0 37.0 37.0 37.0 37.0 85-89 35.8399 37.0 37.0 37.0 37.0 37.0 90-94 35.861200000000004 37.0 37.0 37.0 37.0 37.0 95-99 35.705799999999996 37.0 37.0 37.0 37.0 37.0 100-104 35.7236 37.0 37.0 37.0 37.0 37.0 105-109 35.59599999999999 37.0 37.0 37.0 37.0 37.0 110-114 35.5967 37.0 37.0 37.0 37.0 37.0 115-119 35.4839 37.0 37.0 37.0 37.0 37.0 120-124 35.4771 37.0 37.0 37.0 37.0 37.0 125-129 35.5465 37.0 37.0 37.0 37.0 37.0 130-134 35.3607 37.0 37.0 37.0 34.6 37.0 135-139 35.231399999999994 37.0 37.0 37.0 27.4 37.0 140-144 35.1601 37.0 37.0 37.0 25.0 37.0 145-149 35.2373 37.0 37.0 37.0 29.8 37.0 150 35.096 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 0.0 15 0.0 16 1.0 17 1.0 18 0.0 19 1.0 20 2.0 21 3.0 22 1.0 23 4.0 24 7.0 25 7.0 26 12.0 27 17.0 28 22.0 29 21.0 30 42.0 31 63.0 32 78.0 33 90.0 34 183.0 35 469.0 36 2746.0 37 229.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 30.010147133434806 23.059360730593607 18.822932521562656 28.107559614408927 2 29.875 24.575 27.224999999999998 18.325 3 23.825 26.25 27.400000000000002 22.525000000000002 4 25.650000000000002 27.825 24.875 21.65 5 25.15 28.549999999999997 25.874999999999996 20.424999999999997 6 23.025000000000002 31.1 24.675 21.2 7 22.425 21.725 35.775 20.075000000000003 8 23.400000000000002 22.775000000000002 27.85 25.974999999999998 9 23.775 21.224999999999998 30.375000000000004 24.625 10-14 24.925 25.385 26.36 23.330000000000002 15-19 25.064999999999998 23.555 28.67 22.71 20-24 25.2 23.66 28.01 23.13 25-29 25.419999999999998 23.175 28.24 23.165 30-34 25.5 23.11 28.994999999999997 22.395 35-39 26.06 23.45 28.07 22.42 40-44 24.415 24.46 27.725 23.400000000000002 45-49 25.385 24.025 28.22 22.37 50-54 25.074999999999996 24.83 26.63 23.465 55-59 25.19 23.97 26.884999999999998 23.955000000000002 60-64 24.075 23.5 28.515 23.91 65-69 26.055 24.310000000000002 26.605 23.03 70-74 25.900000000000002 24.37 27.250000000000004 22.48 75-79 25.305 23.825 27.634999999999998 23.235 80-84 25.19 24.404999999999998 27.85 22.555 85-89 25.585 23.765 27.265 23.385 90-94 24.575 24.125 27.58 23.72 95-99 24.335 23.94 28.095 23.630000000000003 100-104 25.445 23.885 28.555000000000003 22.115000000000002 105-109 25.779999999999998 23.974999999999998 27.644999999999996 22.6 110-114 24.695 24.474999999999998 27.66 23.169999999999998 115-119 25.025 24.68 27.235 23.06 120-124 25.505 23.775 27.939999999999998 22.78 125-129 24.154999999999998 25.31 27.235 23.3 130-134 24.345 25.380000000000003 26.86 23.415 135-139 24.945 24.065 28.42 22.57 140-144 25.174999999999997 24.075 28.33 22.42 145-149 24.525 23.335 28.610000000000003 23.53 150 24.075 24.125 27.425 24.375 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 1.5 24 3.5 25 9.0 26 10.5 27 6.5 28 6.0 29 11.5 30 16.5 31 20.0 32 25.0 33 33.5 34 42.0 35 55.5 36 93.0 37 138.5 38 159.0 39 166.0 40 183.0 41 198.5 42 192.0 43 198.0 44 204.5 45 200.0 46 204.5 47 164.0 48 130.5 49 117.5 50 92.5 51 78.5 52 76.5 53 65.0 54 49.0 55 43.5 56 46.0 57 44.5 58 38.5 59 45.5 60 54.5 61 52.5 62 45.5 63 71.0 64 88.0 65 73.5 66 53.0 67 38.0 68 44.0 69 48.5 70 39.0 71 27.5 72 32.0 73 35.5 74 27.0 75 23.5 76 18.5 77 16.5 78 12.5 79 9.0 80 8.0 81 4.0 82 2.0 83 0.5 84 1.0 85 1.5 86 0.5 87 0.5 88 1.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.4500000000000002 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.14999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 83.8950097213221 64.725 2 10.920285158781594 16.85 3 2.4951393389500973 5.775 4 1.1017498379779649 3.4000000000000004 5 0.6156837329876863 2.375 6 0.22683084899546338 1.05 7 0.16202203499675957 0.8750000000000001 8 0.12961762799740764 0.8 9 0.09721322099805574 0.675 >10 0.356448476992871 3.4750000000000005 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 27 0.675 No Hit ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 15 0.375 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 12 0.3 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 12 0.3 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 12 0.3 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 11 0.27499999999999997 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 10 0.25 No Hit TCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTG 10 0.25 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 10 0.25 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 10 0.25 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 10 0.25 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 9 0.22499999999999998 No Hit CAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTC 9 0.22499999999999998 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 9 0.22499999999999998 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 8 0.2 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 8 0.2 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 8 0.2 No Hit AGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGC 8 0.2 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 7 0.17500000000000002 No Hit CTATTGGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGT 7 0.17500000000000002 No Hit AACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGG 7 0.17500000000000002 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 7 0.17500000000000002 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 7 0.17500000000000002 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 6 0.15 No Hit TAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCC 6 0.15 No Hit AGTAGCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACG 6 0.15 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 6 0.15 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 6 0.15 No Hit GTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAG 6 0.15 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 6 0.15 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 5 0.125 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 5 0.125 No Hit GACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCC 5 0.125 No Hit AATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGC 5 0.125 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 5 0.125 No Hit CTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTG 5 0.125 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 5 0.125 No Hit AGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACT 5 0.125 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 5 0.125 No Hit AAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATC 5 0.125 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 5 0.125 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 5 0.125 No Hit CACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTT 5 0.125 No Hit GGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTA 5 0.125 No Hit CTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTA 5 0.125 No Hit CTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGG 5 0.125 No Hit GAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCG 5 0.125 No Hit GCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGC 5 0.125 No Hit TGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.037500000000000006 0.0 0.0 0.0 0.0 90-91 0.05 0.0 0.0 0.0 0.0 92-93 0.075 0.0 0.0 0.0 0.0 94-95 0.075 0.0 0.0 0.0 0.0 96-97 0.075 0.0 0.0 0.0 0.0 98-99 0.075 0.0 0.0 0.0 0.0 100-101 0.0875 0.0 0.0 0.0 0.0 102-103 0.1 0.0 0.0 0.0 0.0 104-105 0.1125 0.0 0.0 0.0 0.0 106-107 0.16249999999999998 0.0 0.0 0.0 0.0 108-109 0.175 0.0 0.0 0.0 0.0 110-111 0.2 0.0 0.0 0.0 0.0 112-113 0.2375 0.0 0.0 0.0 0.0 114-115 0.35 0.0 0.0 0.0 0.0 116-117 0.44999999999999996 0.0 0.0 0.0 0.0 118-119 0.5375 0.0 0.0 0.0 0.0 120-121 0.6 0.0 0.0 0.0 0.0 122-123 0.625 0.0 0.0 0.0 0.0 124-125 0.65 0.0 0.0 0.0 0.0 126-127 0.6875 0.0 0.0 0.0 0.0 128-129 0.7625 0.0 0.0 0.0 0.0 130-131 0.8374999999999999 0.0 0.0 0.0 0.0 132-133 0.9125000000000001 0.0 0.0 0.0 0.0 134-135 1.0125 0.0 0.0 0.0 0.0 136-137 1.0625 0.0 0.0 0.0 0.0 138 1.15 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAATAAT 10 0.0067147487 145.81013 1 TCATGGC 10 0.0069754543 143.9875 9 TATCATG 10 0.0069754543 143.9875 7 TAATATC 10 0.0069754543 143.9875 4 ATCATGG 10 0.0069754543 143.9875 8 AATAATA 10 0.0069754543 143.9875 2 >>END_MODULE Read 2588631 spots for ERR11006598.sra Written 2588631 spots for ERR11006598.sra Read 2588631 spots for ERR11006598.sra Written 2588631 spots for ERR11006598.sra Read 2588631 spots for ERR11006598.sra Written 2588631 spots for ERR11006598.sra Read 2588631 spots for ERR11006598.sra Written 2588631 spots for ERR11006598.sra Read 2588631 spots for ERR11006598.sra Written 2588631 spots for ERR11006598.sra Read 2588631 spots for ERR11006598.sra Written 2588631 spots for ERR11006598.sra Read 2588631 spots for ERR11006598.sra Written 2588631 spots for ERR11006598.sra Read 2588631 spots for ERR11006598.sra Written 2588631 spots for ERR11006598.sra Read 2588631 spots for ERR11006598.sra Written 2588631 spots for ERR11006598.sra Read 2588631 spots for ERR11006598.sra Written 2588631 spots for ERR11006598.sra Read 2588631 spots for ERR11006598.sra Written 2588631 spots for ERR11006598.sra Read 2588631 spots for ERR11006598.sra Written 2588631 spots for ERR11006598.sra Read 2588631 spots for ERR11006598.sra Written 2588631 spots for ERR11006598.sra Read 2588631 spots for ERR11006598.sra Written 2588631 spots for ERR11006598.sra Read 2588631 spots for ERR11006598.sra Written 2588631 spots for ERR11006598.sra Read 2588631 spots for ERR11006598.sra Written 2588631 spots for ERR11006598.sra Read 2588631 spots for ERR11006598.sra Written 2588631 spots for ERR11006598.sra Read 2588631 spots for ERR11006598.sra Written 2588631 spots for ERR11006598.sra Read 2588631 spots for ERR11006598.sra Written 2588631 spots for ERR11006598.sra Read 2588631 spots for ERR11006598.sra Written 2588631 spots for ERR11006598.sra SRR ids: ['ERR11006598.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_66mba5ox ERR11006598.sra spots: 51772620 blocks: [[1, 2588631], [2588632, 5177262], [5177263, 7765893], [7765894, 10354524], [10354525, 12943155], [12943156, 15531786], [15531787, 18120417], [18120418, 20709048], [20709049, 23297679], [23297680, 25886310], [25886311, 28474941], [28474942, 31063572], [31063573, 33652203], [33652204, 36240834], [36240835, 38829465], [38829466, 41418096], [41418097, 44006727], [44006728, 46595358], [46595359, 49183989], [49183990, 51772620]] ERR11006598 file size 19022948 ERR11006598 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006598 ERR11006598_1.fastq ERR11006598_2.fastq Input file: ERR11006598_1.fastq Paired file: ERR11006598_2.fastq trimmed: ERR11006598-trimmed-pair1.fastq, ERR11006598-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 19:07:16 2024 >> started Fri Dec 6 19:08:39 2024 >> done (83.611s) 51772620 read pairs processed; of these: 181 ( 0.00%) short read pairs filtered out after trimming by size control 1588 ( 0.00%) empty read pairs filtered out after trimming by size control 51770851 (100.00%) read pairs available; of these: 738777 ( 1.43%) trimmed read pairs available after processing 51032074 (98.57%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 11 0.00% 19 10 0.00% 20 9 0.00% 21 11 0.00% 22 22 0.00% 23 17 0.00% 24 15 0.00% 25 30 0.00% 26 24 0.00% 27 23 0.00% 28 18 0.00% 29 28 0.00% 30 26 0.00% 31 92 0.00% 32 31 0.00% 33 36 0.00% 34 27 0.00% 35 41 0.00% 36 29 0.00% 37 35 0.00% 38 36 0.00% 39 54 0.00% 40 41 0.00% 41 52 0.00% 42 34 0.00% 43 54 0.00% 44 52 0.00% 45 78 0.00% 46 60 0.00% 47 71 0.00% 48 83 0.00% 49 88 0.00% 50 94 0.00% 51 101 0.00% 52 114 0.00% 53 126 0.00% 54 123 0.00% 55 135 0.00% 56 141 0.00% 57 168 0.00% 58 187 0.00% 59 189 0.00% 60 195 0.00% 61 252 0.00% 62 287 0.00% 63 289 0.00% 64 335 0.00% 65 320 0.00% 66 333 0.00% 67 382 0.00% 68 394 0.00% 69 400 0.00% 70 448 0.00% 71 497 0.00% 72 563 0.00% 73 629 0.00% 74 686 0.00% 75 728 0.00% 76 741 0.00% 77 834 0.00% 78 891 0.00% 79 911 0.00% 80 1010 0.00% 81 1088 0.00% 82 1161 0.00% 83 1268 0.00% 84 1437 0.00% 85 1582 0.00% 86 1595 0.00% 87 1785 0.00% 88 1884 0.00% 89 2118 0.00% 90 2194 0.00% 91 2224 0.00% 92 2354 0.00% 93 2470 0.00% 94 2646 0.01% 95 2915 0.01% 96 3090 0.01% 97 3276 0.01% 98 3450 0.01% 99 3589 0.01% 100 3768 0.01% 101 3910 0.01% 102 4027 0.01% 103 4255 0.01% 104 4530 0.01% 105 4775 0.01% 106 5062 0.01% 107 5318 0.01% 108 5817 0.01% 109 5850 0.01% 110 6406 0.01% 111 6597 0.01% 112 6940 0.01% 113 7285 0.01% 114 7405 0.01% 115 7970 0.02% 116 8601 0.02% 117 8873 0.02% 118 9163 0.02% 119 9719 0.02% 120 10212 0.02% 121 10472 0.02% 122 11069 0.02% 123 11580 0.02% 124 12007 0.02% 125 13039 0.03% 126 12887 0.02% 127 12770 0.02% 128 13547 0.03% 129 14323 0.03% 130 15287 0.03% 131 16304 0.03% 132 16504 0.03% 133 16228 0.03% 134 17166 0.03% 135 17486 0.03% 136 18299 0.04% 137 18677 0.04% 138 19455 0.04% 139 20624 0.04% 140 21452 0.04% 141 22794 0.04% 142 23235 0.04% 143 24254 0.05% 144 24693 0.05% 145 25817 0.05% 146 28058 0.05% 147 28031 0.05% 148 29417 0.06% 149 30952 0.06% 150 51032074 98.57% 51770851 reads passed initial QC criterion=sequence-density sequence-density=0.24 sequence-density-rank=1 fanout-score=4.55 fanout-score-rank=18 prefix-density=0.33 prefix-fanout=3.3 sequence=GCAGGTGCAGCTGGTGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=37 fanout-score=65.84 fanout-score-rank=1 prefix-density=0.13 prefix-fanout=4.4 sequence=TAAAAAAAAGGGGGGGTAAGGACCCGCTAAGCTCCTACTTTTTCATGTTTCCAATCCGATCCCTCCGATTACTATAGAGATGAACCCAATCCAGAATATGAACCATAAAAGAAAACACCTACTAAACCAATCACAAGAATACCAGTTACCGTACCTATCAGCCAAAGAGGAATTCTTCCAGTAGTATCGGCCATTTCCCCTACTTTCCTCCACATTTTATCAAGTGGTCATGCTAGAGACAAAAACAGTCATGGATAGTTATGTTATAAGGATGGTATCCTTCCAAATGGGATAAGAGAGTTCTTACTACTCTCTTCTTTTCTCTCAATTGAAGAAGTAATTGGAAAACAAAACAGCAAGTACAAAAATGAGTAATAAACCCCAGTATAGACTGGTACGATTCAATTCAACATTTTGTTCATTCGGGTTTGATTGTGTCATAGTTCTATAGTTGGAATTTAGTTTATCGTTGGATGAACTGCATTGCTGATATTGATCCCAAGAAAAAAAC criterion=sequence-density sequence-density=0.28 sequence-density-rank=1 fanout-score=9.90 fanout-score-rank=11 prefix-density=2.81 prefix-fanout=1.0 sequence=TTGCGTAGTGGATCTGCTGGGGCCTATGCGAAAGCTGGGCCTCACGAATTCTATAGTGGCAGGCACCGCGTTAGGCTGGCTTC criterion=fanout-score sequence-density=0.06 sequence-density-rank=23 fanout-score=166.74 fanout-score-rank=1 prefix-density=0.44 prefix-fanout=23.6 sequence=CGCCGCCGCCGG ERR11006598 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 19:11:32 Started mapping on | Dec 06 19:11:32 Finished on | Dec 06 19:18:11 Mapping speed, Million of reads per hour | 467.11 Number of input reads | 51770851 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 43449661 Uniquely mapped reads % | 83.93% Average mapped length | 298.16 Number of splices: Total | 21879058 Number of splices: Annotated (sjdb) | 20502908 Number of splices: GT/AG | 21562513 Number of splices: GC/AG | 234108 Number of splices: AT/AC | 9024 Number of splices: Non-canonical | 73413 Mismatch rate per base, % | 0.30% Deletion rate per base | 0.02% Deletion average length | 1.87 Insertion rate per base | 0.01% Insertion average length | 2.20 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 6272569 % of reads mapped to multiple loci | 12.12% Number of reads mapped to too many loci | 18355 % of reads mapped to too many loci | 0.04% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.16% % of reads unmapped: other | 0.76% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2048621 2048621 2048621 N_multimapping 6272569 6272569 6272569 N_noFeature 8649224 39704740 11542073 N_ambiguous 1194172 40025 321005 UnstrandedReadsAssigned:33606265 PositiveStrandReadsAssigned:3704896 NegativeStrandReadsAssigned:31586583 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006598 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006598-trimmed-pair1.fastq ERR11006598-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 51,770,851 reads, 32,989,294 reads pseudoaligned [quant] estimated average fragment length: 269.464 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,162 rounds 52973 ERR11006598.ke.tsv 35125 ERR11006598.se.tsv 88098 total ==> ERR11006598.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 667.947 0 0 PNS24247 1044 775.536 31.4914 1.49886 PNS24249 1928 1659.54 433.355 9.63895 PNS24246 1044 775.536 31.4914 1.49886 PNS24248 1044 775.536 31.4914 1.49886 PNS24244 1471 1202.54 91.1704 2.79852 PNS24243 293 55.2348 2 1.33656 KQK14069 1603 1334.54 528.457 14.6168 KQK14071 474 208.221 12.6187 2.23699 ==> ERR11006598.se.tsv <== BRADI_1g14170v3 601 BRADI_1g53295v3 1728 BRADI_1g59795v3 354 BRADI_1g07683v3 0 BRADI_1g00485v3 13 BRADI_1g20270v3 693 BRADI_1g74790v3 1334 BRADI_1g09890v3 1 BRADI_1g77505v3 142 BRADI_1g48960v3 1 ERR11006598 completed mapping pipeline successfully