Starting /dee2/code/volunteer_pipeline.sh ERR11006599 current disk space = 1550101360640 free memory = 1601859216 ERR11006599 SRAfilesize f325bd265988b1741aa19fbc3ccdff48 ERR11006599.sra ERR11006599.sra file validated ERR11006599 is paired end ERR11006599 is conventional basespace ERR11006599 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006599_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2995 37.0 37.0 37.0 37.0 37.0 2 36.1625 37.0 37.0 37.0 37.0 37.0 3 36.16 37.0 37.0 37.0 37.0 37.0 4 36.302 37.0 37.0 37.0 37.0 37.0 5 36.4065 37.0 37.0 37.0 37.0 37.0 6 36.397 37.0 37.0 37.0 37.0 37.0 7 36.2465 37.0 37.0 37.0 37.0 37.0 8 36.3225 37.0 37.0 37.0 37.0 37.0 9 36.4565 37.0 37.0 37.0 37.0 37.0 10-14 36.374700000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.3775 37.0 37.0 37.0 37.0 37.0 20-24 36.3309 37.0 37.0 37.0 37.0 37.0 25-29 36.2947 37.0 37.0 37.0 37.0 37.0 30-34 36.2324 37.0 37.0 37.0 37.0 37.0 35-39 36.2613 37.0 37.0 37.0 37.0 37.0 40-44 36.184900000000006 37.0 37.0 37.0 37.0 37.0 45-49 36.1267 37.0 37.0 37.0 37.0 37.0 50-54 36.1481 37.0 37.0 37.0 37.0 37.0 55-59 36.132999999999996 37.0 37.0 37.0 37.0 37.0 60-64 36.0788 37.0 37.0 37.0 37.0 37.0 65-69 36.1178 37.0 37.0 37.0 37.0 37.0 70-74 35.9896 37.0 37.0 37.0 37.0 37.0 75-79 35.93820000000001 37.0 37.0 37.0 37.0 37.0 80-84 35.93730000000001 37.0 37.0 37.0 37.0 37.0 85-89 35.932100000000005 37.0 37.0 37.0 37.0 37.0 90-94 35.836 37.0 37.0 37.0 37.0 37.0 95-99 35.8529 37.0 37.0 37.0 37.0 37.0 100-104 35.826800000000006 37.0 37.0 37.0 37.0 37.0 105-109 35.8214 37.0 37.0 37.0 37.0 37.0 110-114 35.666 37.0 37.0 37.0 37.0 37.0 115-119 35.6675 37.0 37.0 37.0 37.0 37.0 120-124 35.6575 37.0 37.0 37.0 37.0 37.0 125-129 35.608999999999995 37.0 37.0 37.0 37.0 37.0 130-134 35.4422 37.0 37.0 37.0 34.6 37.0 135-139 35.4092 37.0 37.0 37.0 37.0 37.0 140-144 35.2031 37.0 37.0 37.0 29.8 37.0 145-149 35.3738 37.0 37.0 37.0 37.0 37.0 150 35.192 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 0.0 21 1.0 22 0.0 23 7.0 24 4.0 25 4.0 26 7.0 27 24.0 28 24.0 29 43.0 30 57.0 31 64.0 32 80.0 33 115.0 34 141.0 35 300.0 36 2795.0 37 333.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.85 14.825 14.725 34.599999999999994 2 29.7 10.375 27.875 32.05 3 23.0 13.725000000000001 26.525 36.75 4 27.500000000000004 15.525 23.525 33.45 5 28.575 20.4 24.625 26.400000000000002 6 25.900000000000002 27.975 21.8 24.325 7 16.650000000000002 29.675 34.65 19.025 8 17.1 29.2 31.45 22.25 9 18.099999999999998 28.525 31.85 21.525 10-14 20.935000000000002 31.41 26.33 21.325 15-19 21.34 31.06 24.62 22.98 20-24 21.44 28.884999999999998 26.58 23.095 25-29 22.46 30.25 24.935 22.355 30-34 23.044999999999998 30.42 24.465 22.07 35-39 22.915 29.285 25.419999999999998 22.38 40-44 21.97 29.049999999999997 25.490000000000002 23.49 45-49 21.65 28.78 26.27 23.3 50-54 21.275 31.19 25.119999999999997 22.415 55-59 21.945 28.92 24.7 24.435000000000002 60-64 21.23 29.244999999999997 25.919999999999998 23.605 65-69 21.695 29.37 24.89 24.044999999999998 70-74 22.175 28.685 24.035 25.105 75-79 22.835 29.375 24.9 22.89 80-84 22.555 28.26 24.84 24.345 85-89 22.965 28.449999999999996 25.52 23.064999999999998 90-94 22.09 28.82 25.119999999999997 23.97 95-99 23.119999999999997 28.735 24.13 24.015 100-104 23.145 28.860000000000003 24.57 23.425 105-109 22.3 28.115000000000002 25.619999999999997 23.965 110-114 22.54 28.494999999999997 24.685000000000002 24.279999999999998 115-119 21.279999999999998 29.580000000000002 25.0 24.14 120-124 20.974999999999998 29.765000000000004 24.135 25.124999999999996 125-129 21.46 30.145 23.555 24.84 130-134 22.675 29.715000000000003 23.82 23.79 135-139 23.330000000000002 29.2 23.43 24.04 140-144 24.115000000000002 28.810000000000002 24.905 22.17 145-149 23.35 28.535 24.755 23.36 150 23.325000000000003 26.450000000000003 26.525 23.7 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.0 19 0.0 20 0.0 21 0.0 22 1.0 23 2.5 24 4.5 25 5.5 26 6.5 27 8.5 28 10.5 29 14.0 30 19.5 31 28.5 32 30.0 33 32.5 34 39.5 35 50.5 36 85.5 37 149.5 38 235.0 39 235.5 40 205.5 41 241.5 42 260.5 43 235.5 44 209.5 45 212.0 46 199.5 47 160.0 48 134.0 49 114.0 50 95.5 51 74.0 52 65.0 53 59.5 54 44.5 55 44.0 56 38.5 57 39.0 58 36.0 59 28.0 60 30.0 61 26.0 62 28.0 63 38.5 64 53.0 65 61.0 66 42.0 67 29.5 68 34.0 69 34.0 70 26.5 71 22.0 72 21.5 73 16.5 74 12.0 75 11.0 76 12.5 77 12.5 78 9.5 79 6.5 80 5.0 81 4.0 82 3.0 83 2.5 84 1.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.9 #Duplication Level Percentage of deduplicated Percentage of total 1 83.22056833558862 61.5 2 11.400541271989175 16.85 3 2.469553450608931 5.475 4 1.1163734776725303 3.3000000000000003 5 0.4397834912043302 1.625 6 0.3044654939106901 1.35 7 0.20297699594046006 1.05 8 0.16914749661705006 1.0 9 0.2368064952638701 1.575 >10 0.40595399188092013 4.55 >50 0.03382949932341001 1.725 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 69 1.725 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 19 0.475 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 19 0.475 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 19 0.475 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 17 0.42500000000000004 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 17 0.42500000000000004 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 14 0.35000000000000003 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 14 0.35000000000000003 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 14 0.35000000000000003 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 14 0.35000000000000003 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 13 0.325 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 12 0.3 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 10 0.25 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 9 0.22499999999999998 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 9 0.22499999999999998 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 9 0.22499999999999998 No Hit TGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATA 9 0.22499999999999998 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 9 0.22499999999999998 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 9 0.22499999999999998 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 9 0.22499999999999998 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 8 0.2 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 8 0.2 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 8 0.2 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 8 0.2 No Hit CTTCCATACCAAGATTAGCACGGTTGATGATATCAGCCCAAGTATTAATA 8 0.2 No Hit ACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 7 0.17500000000000002 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 7 0.17500000000000002 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 7 0.17500000000000002 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 7 0.17500000000000002 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 7 0.17500000000000002 No Hit CACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAAT 7 0.17500000000000002 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 6 0.15 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 6 0.15 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 6 0.15 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 6 0.15 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 6 0.15 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 6 0.15 No Hit TAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGA 6 0.15 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 6 0.15 No Hit AGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGG 6 0.15 No Hit TCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCG 5 0.125 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 5 0.125 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 5 0.125 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 5 0.125 No Hit TGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 5 0.125 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 5 0.125 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 5 0.125 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 5 0.125 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 5 0.125 No Hit ATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATA 5 0.125 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 5 0.125 No Hit CACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCG 5 0.125 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.025 0.0 0.0 0.0 0.0 92-93 0.025 0.0 0.0 0.0 0.0 94-95 0.025 0.0 0.0 0.0 0.0 96-97 0.025 0.0 0.0 0.0 0.0 98-99 0.025 0.0 0.0 0.0 0.0 100-101 0.025 0.0 0.0 0.0 0.0 102-103 0.025 0.0 0.0 0.0 0.0 104-105 0.025 0.0 0.0 0.0 0.0 106-107 0.025 0.0 0.0 0.0 0.0 108-109 0.025 0.0 0.0 0.0 0.0 110-111 0.05 0.0 0.0 0.0 0.0 112-113 0.05 0.0 0.0 0.0 0.0 114-115 0.0625 0.0 0.0 0.0 0.0 116-117 0.1 0.0 0.0 0.0 0.0 118-119 0.1 0.0 0.0 0.0 0.0 120-121 0.1 0.0 0.0 0.0 0.0 122-123 0.125 0.0 0.0 0.0 0.0 124-125 0.125 0.0 0.0 0.0 0.0 126-127 0.1375 0.0 0.0 0.0 0.0 128-129 0.15 0.0 0.0 0.0 0.0 130-131 0.15 0.0 0.0 0.0 0.0 132-133 0.21250000000000002 0.0 0.0 0.0 0.0 134-135 0.2875 0.0 0.0 0.0 0.0 136-137 0.3 0.0 0.0 0.0 0.0 138 0.3 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTTTC 30 0.0015031899 23.999998 35-39 CGATAAT 30 0.0015031899 23.999998 110-114 GCGATGA 30 0.0015031899 23.999998 100-104 AAACAGG 30 0.0015031899 23.999998 60-64 GCGATAA 30 0.0015031899 23.999998 110-114 TGCGATG 30 0.0015031899 23.999998 100-104 GCGAATA 30 0.0015031899 23.999998 70-74 GCGGTCA 30 0.0015031899 23.999998 130-134 TGTTTCC 30 0.0015031899 23.999998 35-39 TCAATAT 30 0.0015031899 23.999998 80-84 GCTCGCG 30 0.0015031899 23.999998 65-69 CTGGAGG 30 0.0015031899 23.999998 90-94 TGAAGGC 30 0.0015031899 23.999998 105-109 AAGGTAG 30 0.0015031899 23.999998 140-144 TCCATAA 30 0.0015031899 23.999998 40-44 TAATATT 30 0.0015031899 23.999998 30-34 GGGGCTG 30 0.0015031899 23.999998 95-99 AATATTG 30 0.0015031899 23.999998 30-34 AACAGGC 30 0.0015031899 23.999998 60-64 AGGTAGG 30 0.0015031899 23.999998 140-144 >>END_MODULE ERR11006599 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006599_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.4795 37.0 37.0 37.0 37.0 37.0 2 36.117 37.0 37.0 37.0 37.0 37.0 3 36.206 37.0 37.0 37.0 37.0 37.0 4 36.0785 37.0 37.0 37.0 37.0 37.0 5 36.261 37.0 37.0 37.0 37.0 37.0 6 36.2215 37.0 37.0 37.0 37.0 37.0 7 36.191 37.0 37.0 37.0 37.0 37.0 8 36.298 37.0 37.0 37.0 37.0 37.0 9 36.218 37.0 37.0 37.0 37.0 37.0 10-14 36.230599999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.2503 37.0 37.0 37.0 37.0 37.0 20-24 36.2302 37.0 37.0 37.0 37.0 37.0 25-29 36.1922 37.0 37.0 37.0 37.0 37.0 30-34 36.1288 37.0 37.0 37.0 37.0 37.0 35-39 36.1292 37.0 37.0 37.0 37.0 37.0 40-44 36.1083 37.0 37.0 37.0 37.0 37.0 45-49 36.07790000000001 37.0 37.0 37.0 37.0 37.0 50-54 36.0482 37.0 37.0 37.0 37.0 37.0 55-59 36.0449 37.0 37.0 37.0 37.0 37.0 60-64 35.8985 37.0 37.0 37.0 37.0 37.0 65-69 35.9369 37.0 37.0 37.0 37.0 37.0 70-74 35.8817 37.0 37.0 37.0 37.0 37.0 75-79 35.8787 37.0 37.0 37.0 37.0 37.0 80-84 35.800200000000004 37.0 37.0 37.0 37.0 37.0 85-89 35.7467 37.0 37.0 37.0 37.0 37.0 90-94 35.783300000000004 37.0 37.0 37.0 37.0 37.0 95-99 35.59310000000001 37.0 37.0 37.0 37.0 37.0 100-104 35.574200000000005 37.0 37.0 37.0 37.0 37.0 105-109 35.5344 37.0 37.0 37.0 37.0 37.0 110-114 35.4221 37.0 37.0 37.0 34.6 37.0 115-119 35.3651 37.0 37.0 37.0 37.0 37.0 120-124 35.301 37.0 37.0 37.0 29.8 37.0 125-129 35.3727 37.0 37.0 37.0 37.0 37.0 130-134 35.1453 37.0 37.0 37.0 27.4 37.0 135-139 35.1322 37.0 37.0 37.0 27.4 37.0 140-144 35.104 37.0 37.0 37.0 25.0 37.0 145-149 35.0325 37.0 37.0 37.0 25.0 37.0 150 34.575 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 0.0 15 1.0 16 0.0 17 0.0 18 1.0 19 2.0 20 2.0 21 4.0 22 4.0 23 5.0 24 7.0 25 10.0 26 14.0 27 15.0 28 23.0 29 32.0 30 48.0 31 54.0 32 59.0 33 113.0 34 226.0 35 494.0 36 2708.0 37 177.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.83732789393167 25.803161652218254 17.338092809790922 28.021417644059156 2 27.275 25.424999999999997 28.999999999999996 18.3 3 21.224999999999998 28.075 30.85 19.85 4 24.375 28.499999999999996 26.075 21.05 5 26.950000000000003 27.500000000000004 26.924999999999997 18.625 6 22.8 31.65 24.95 20.599999999999998 7 21.75 21.224999999999998 37.55 19.475 8 22.650000000000002 24.05 29.45 23.849999999999998 9 23.5 19.950000000000003 33.25 23.3 10-14 24.84 26.240000000000002 27.395000000000003 21.525 15-19 24.805 24.585 28.935 21.675 20-24 24.575 24.195 28.689999999999998 22.54 25-29 24.735 24.64 28.945 21.68 30-34 24.8 24.585 28.775000000000002 21.84 35-39 24.945 24.625 28.105000000000004 22.325 40-44 24.240000000000002 25.540000000000003 27.74 22.48 45-49 23.74 25.650000000000002 28.185 22.425 50-54 23.555 25.91 27.694999999999997 22.84 55-59 24.41 24.815 28.07 22.705000000000002 60-64 23.575 24.959999999999997 28.705000000000002 22.759999999999998 65-69 24.73 24.335 27.865000000000002 23.07 70-74 24.104999999999997 25.240000000000002 28.825 21.83 75-79 24.315 24.455 29.275000000000002 21.955 80-84 24.87 24.610000000000003 28.585 21.935 85-89 24.815 25.224999999999998 27.700000000000003 22.259999999999998 90-94 23.695 24.98 28.585 22.74 95-99 24.104999999999997 24.535 28.62 22.74 100-104 24.695 24.425 29.134999999999998 21.745 105-109 24.895 24.135 28.555000000000003 22.415 110-114 24.305 24.625 27.944999999999997 23.125 115-119 25.009999999999998 24.605 28.08 22.305 120-124 23.91 24.815 28.449999999999996 22.825 125-129 23.294999999999998 24.91 29.07 22.725 130-134 23.48 25.135 28.345 23.04 135-139 23.544999999999998 25.005 29.330000000000002 22.12 140-144 23.79 25.055 29.17 21.985 145-149 23.91 24.169999999999998 29.04 22.88 150 23.674999999999997 24.099999999999998 28.95 23.275000000000002 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 1.0 19 0.5 20 0.0 21 0.5 22 2.0 23 1.5 24 4.0 25 9.0 26 9.5 27 10.5 28 13.0 29 15.0 30 18.5 31 28.0 32 34.5 33 43.0 34 54.0 35 63.0 36 94.0 37 163.0 38 196.5 39 199.0 40 211.5 41 217.0 42 211.5 43 219.0 44 228.0 45 209.5 46 185.5 47 152.0 48 134.5 49 112.5 50 84.5 51 74.5 52 62.5 53 50.0 54 39.5 55 36.5 56 40.5 57 39.0 58 36.0 59 37.5 60 45.0 61 41.5 62 37.5 63 53.0 64 64.0 65 63.5 66 51.5 67 39.0 68 32.5 69 26.5 70 26.5 71 27.0 72 25.5 73 25.5 74 25.0 75 17.0 76 10.5 77 10.5 78 8.5 79 5.0 80 6.5 81 7.5 82 4.5 83 3.0 84 1.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.95 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.25 #Duplication Level Percentage of deduplicated Percentage of total 1 82.72131147540983 63.075 2 11.934426229508196 18.2 3 2.459016393442623 5.625 4 1.2786885245901638 3.9 5 0.5573770491803279 2.125 6 0.39344262295081966 1.7999999999999998 7 0.19672131147540983 1.05 8 0.13114754098360656 0.8 9 0.03278688524590164 0.22499999999999998 >10 0.29508196721311475 3.2 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 31 0.775 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 15 0.375 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 15 0.375 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 12 0.3 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 12 0.3 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 12 0.3 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 11 0.27499999999999997 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 10 0.25 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 10 0.25 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 9 0.22499999999999998 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 8 0.2 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 8 0.2 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 8 0.2 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 8 0.2 No Hit AAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATC 7 0.17500000000000002 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 7 0.17500000000000002 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 7 0.17500000000000002 No Hit CAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTA 7 0.17500000000000002 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 7 0.17500000000000002 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 7 0.17500000000000002 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 6 0.15 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 6 0.15 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 6 0.15 No Hit GATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTC 6 0.15 No Hit GCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGC 6 0.15 No Hit ACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGA 6 0.15 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 6 0.15 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 6 0.15 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 6 0.15 No Hit CTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTAC 6 0.15 No Hit CTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGT 6 0.15 No Hit GTGGACGTTGCCGTAGCGCTGCGGGCCTGGTCTGGGTGTGCTACTGATGG 6 0.15 No Hit GAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGA 5 0.125 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 5 0.125 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 5 0.125 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 5 0.125 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 5 0.125 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 5 0.125 No Hit GTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTC 5 0.125 No Hit AATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGC 5 0.125 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 5 0.125 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 5 0.125 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 5 0.125 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 5 0.125 No Hit TAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAG 5 0.125 No Hit GCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGT 5 0.125 No Hit GCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGC 5 0.125 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 5 0.125 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.025 0.0 0.0 0.0 0.0 92-93 0.025 0.0 0.0 0.0 0.0 94-95 0.025 0.0 0.0 0.0 0.0 96-97 0.025 0.0 0.0 0.0 0.0 98-99 0.025 0.0 0.0 0.0 0.0 100-101 0.025 0.0 0.0 0.0 0.0 102-103 0.025 0.0 0.0 0.0 0.0 104-105 0.025 0.0 0.0 0.0 0.0 106-107 0.025 0.0 0.0 0.0 0.0 108-109 0.025 0.0 0.0 0.0 0.0 110-111 0.05 0.0 0.0 0.0 0.0 112-113 0.05 0.0 0.0 0.0 0.0 114-115 0.0625 0.0 0.0 0.0 0.0 116-117 0.1 0.0 0.0 0.0 0.0 118-119 0.1 0.0 0.0 0.0 0.0 120-121 0.1 0.0 0.0 0.0 0.0 122-123 0.125 0.0 0.0 0.0 0.0 124-125 0.125 0.0 0.0 0.0 0.0 126-127 0.125 0.0 0.0 0.0 0.0 128-129 0.125 0.0 0.0 0.0 0.0 130-131 0.125 0.0 0.0 0.0 0.0 132-133 0.1875 0.0 0.0 0.0 0.0 134-135 0.2625 0.0 0.0 0.0 0.0 136-137 0.275 0.0 0.0 0.0 0.0 138 0.275 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 2574379 spots for ERR11006599.sra Written 2574379 spots for ERR11006599.sra Read 2574379 spots for ERR11006599.sra Written 2574379 spots for ERR11006599.sra Read 2574379 spots for ERR11006599.sra Written 2574379 spots for ERR11006599.sra Read 2574379 spots for ERR11006599.sra Written 2574379 spots for ERR11006599.sra Read 2574379 spots for ERR11006599.sra Written 2574379 spots for ERR11006599.sra Read 2574379 spots for ERR11006599.sra Written 2574379 spots for ERR11006599.sra Read 2574388 spots for ERR11006599.sra Written 2574388 spots for ERR11006599.sra Read 2574379 spots for ERR11006599.sra Written 2574379 spots for ERR11006599.sra Read 2574379 spots for ERR11006599.sra Written 2574379 spots for ERR11006599.sra Read 2574379 spots for ERR11006599.sra Written 2574379 spots for ERR11006599.sra Read 2574379 spots for ERR11006599.sra Written 2574379 spots for ERR11006599.sra Read 2574379 spots for ERR11006599.sra Written 2574379 spots for ERR11006599.sra Read 2574379 spots for ERR11006599.sra Written 2574379 spots for ERR11006599.sra Read 2574379 spots for ERR11006599.sra Written 2574379 spots for ERR11006599.sra Read 2574379 spots for ERR11006599.sra Written 2574379 spots for ERR11006599.sra Read 2574379 spots for ERR11006599.sra Written 2574379 spots for ERR11006599.sra Read 2574379 spots for ERR11006599.sra Written 2574379 spots for ERR11006599.sra Read 2574379 spots for ERR11006599.sra Written 2574379 spots for ERR11006599.sra Read 2574379 spots for ERR11006599.sra Written 2574379 spots for ERR11006599.sra Read 2574379 spots for ERR11006599.sra Written 2574379 spots for ERR11006599.sra SRR ids: ['ERR11006599.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_nxhkz2h2 ERR11006599.sra spots: 51487589 blocks: [[1, 2574379], [2574380, 5148758], [5148759, 7723137], [7723138, 10297516], [10297517, 12871895], [12871896, 15446274], [15446275, 18020653], [18020654, 20595032], [20595033, 23169411], [23169412, 25743790], [25743791, 28318169], [28318170, 30892548], [30892549, 33466927], [33466928, 36041306], [36041307, 38615685], [38615686, 41190064], [41190065, 43764443], [43764444, 46338822], [46338823, 48913201], [48913202, 51487589]] ERR11006599 file size 18918170 ERR11006599 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006599 ERR11006599_1.fastq ERR11006599_2.fastq Input file: ERR11006599_1.fastq Paired file: ERR11006599_2.fastq trimmed: ERR11006599-trimmed-pair1.fastq, ERR11006599-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 19:08:45 2024 >> started Fri Dec 6 19:09:58 2024 >> done (72.205s) 51487589 read pairs processed; of these: 296 ( 0.00%) short read pairs filtered out after trimming by size control 4077 ( 0.01%) empty read pairs filtered out after trimming by size control 51483216 (99.99%) read pairs available; of these: 395559 ( 0.77%) trimmed read pairs available after processing 51087657 (99.23%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 9 0.00% 19 8 0.00% 20 7 0.00% 21 12 0.00% 22 19 0.00% 23 17 0.00% 24 7 0.00% 25 23 0.00% 26 26 0.00% 27 25 0.00% 28 22 0.00% 29 20 0.00% 30 21 0.00% 31 142 0.00% 32 20 0.00% 33 30 0.00% 34 10 0.00% 35 23 0.00% 36 27 0.00% 37 22 0.00% 38 33 0.00% 39 31 0.00% 40 31 0.00% 41 36 0.00% 42 25 0.00% 43 42 0.00% 44 40 0.00% 45 38 0.00% 46 42 0.00% 47 55 0.00% 48 58 0.00% 49 60 0.00% 50 45 0.00% 51 61 0.00% 52 61 0.00% 53 81 0.00% 54 75 0.00% 55 78 0.00% 56 96 0.00% 57 85 0.00% 58 104 0.00% 59 125 0.00% 60 128 0.00% 61 137 0.00% 62 141 0.00% 63 154 0.00% 64 193 0.00% 65 166 0.00% 66 194 0.00% 67 188 0.00% 68 203 0.00% 69 273 0.00% 70 256 0.00% 71 277 0.00% 72 279 0.00% 73 306 0.00% 74 326 0.00% 75 369 0.00% 76 353 0.00% 77 424 0.00% 78 506 0.00% 79 420 0.00% 80 493 0.00% 81 512 0.00% 82 551 0.00% 83 601 0.00% 84 620 0.00% 85 726 0.00% 86 803 0.00% 87 846 0.00% 88 847 0.00% 89 942 0.00% 90 1042 0.00% 91 1007 0.00% 92 1116 0.00% 93 1072 0.00% 94 1144 0.00% 95 1287 0.00% 96 1316 0.00% 97 1485 0.00% 98 1602 0.00% 99 1681 0.00% 100 1663 0.00% 101 1895 0.00% 102 1937 0.00% 103 1948 0.00% 104 2138 0.00% 105 2315 0.00% 106 2486 0.00% 107 2596 0.01% 108 2788 0.01% 109 2787 0.01% 110 3012 0.01% 111 3119 0.01% 112 3390 0.01% 113 3372 0.01% 114 3750 0.01% 115 3747 0.01% 116 4032 0.01% 117 4383 0.01% 118 4499 0.01% 119 4729 0.01% 120 5292 0.01% 121 5284 0.01% 122 5529 0.01% 123 5798 0.01% 124 6243 0.01% 125 6778 0.01% 126 6277 0.01% 127 6553 0.01% 128 7038 0.01% 129 7559 0.01% 130 8001 0.02% 131 8533 0.02% 132 8687 0.02% 133 8922 0.02% 134 9122 0.02% 135 9232 0.02% 136 9887 0.02% 137 10443 0.02% 138 11016 0.02% 139 11479 0.02% 140 11679 0.02% 141 12660 0.02% 142 13246 0.03% 143 13791 0.03% 144 13972 0.03% 145 15240 0.03% 146 16744 0.03% 147 16633 0.03% 148 17843 0.03% 149 18714 0.04% 150 51087657 99.23% 51483216 reads passed initial QC criterion=sequence-density sequence-density=0.20 sequence-density-rank=1 fanout-score=0.00 fanout-score-rank=35 prefix-density=0.00 prefix-fanout=1.0 sequence=TGCGGGAACTTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=31 fanout-score=517.38 fanout-score-rank=1 prefix-density=5.15 prefix-fanout=1.0 sequence=AAGTATTAATACAGTCACATGGATGGTAAATGCGTGAATGTGATGGACTAAAAAATCTGCAGTTCCTAATGGAATAGGTAACAAAGCCACTTTGCCGCCTACTGCTACTAACTCGCCACCTCCCCACGTTAAACTGGTACTTGTTGTTGCACCAGGAGCTGTTACACCAGGCGCGTTAGCATGGATATTTTGTACCCATTGAGCAAAGATAGGTTGTAATTGTATGGCAGTATCCGAAAACATATCTTGCGGACGTCCTAAAGCACTCATGGTATCATTATGAATGTACAAACCAAAACTGTGAAAACCTAGAAATATACATACCCAGTTAAGGTGGGATATGATTGCATCACGGTGTCTAAGGACGCGATCTAATAGATCATTGTATCGAGTAGTTGGATCATAGTCTCTTACCATAAAAATTGCTGCATGTGCAGCAGCACCGACTATTAGAAATCCGCCAATCCACATGTGGTGTGTGAACAAGGAAAGTTGTGTACCATAGTCAGTA criterion=sequence-density sequence-density=0.18 sequence-density-rank=1 fanout-score=10.57 fanout-score-rank=6 prefix-density=1.89 prefix-fanout=1.0 sequence=TTGCGTAGTGGATCTGCTGGGGCCTATGCGAAAGCTGGGCCTCACGAATTCTATAGTGGCAGGCACCGCGTTAGGCTGGCTTC criterion=fanout-score sequence-density=0.08 sequence-density-rank=14 fanout-score=58.60 fanout-score-rank=1 prefix-density=0.43 prefix-fanout=11.4 sequence=CCGCCGCCGCCGG ERR11006599 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 19:10:37 Started mapping on | Dec 06 19:10:37 Finished on | Dec 06 19:16:32 Mapping speed, Million of reads per hour | 522.08 Number of input reads | 51483216 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 41024729 Uniquely mapped reads % | 79.69% Average mapped length | 298.37 Number of splices: Total | 19750217 Number of splices: Annotated (sjdb) | 18541416 Number of splices: GT/AG | 19425474 Number of splices: GC/AG | 223765 Number of splices: AT/AC | 17401 Number of splices: Non-canonical | 83577 Mismatch rate per base, % | 0.30% Deletion rate per base | 0.02% Deletion average length | 1.93 Insertion rate per base | 0.01% Insertion average length | 2.10 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 8364566 % of reads mapped to multiple loci | 16.25% Number of reads mapped to too many loci | 16484 % of reads mapped to too many loci | 0.03% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.02% % of reads unmapped: other | 1.02% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2093921 2093921 2093921 N_multimapping 8364566 8364566 8364566 N_noFeature 8577084 37328472 11378167 N_ambiguous 1358451 48725 453525 UnstrandedReadsAssigned:31089194 PositiveStrandReadsAssigned:3647532 NegativeStrandReadsAssigned:29193037 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006599 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006599-trimmed-pair1.fastq ERR11006599-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 51,483,216 reads, 32,100,734 reads pseudoaligned [quant] estimated average fragment length: 272.817 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,320 rounds 52973 ERR11006599.ke.tsv 35125 ERR11006599.se.tsv 88098 total ==> ERR11006599.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 664.664 0 0 PNS24247 1044 772.183 50.7706 2.28204 PNS24249 1928 1656.18 259.748 5.44346 PNS24246 1044 772.183 50.7706 2.28204 PNS24248 1044 772.183 50.7706 2.28204 PNS24244 1471 1199.18 77.9404 2.25584 PNS24243 293 51.083 3 2.03834 KQK14069 1603 1331.18 425.071 11.0829 KQK14071 474 205.086 15.0438 2.54596 ==> ERR11006599.se.tsv <== BRADI_1g14170v3 479 BRADI_1g53295v3 923 BRADI_1g59795v3 412 BRADI_1g07683v3 0 BRADI_1g00485v3 5 BRADI_1g20270v3 694 BRADI_1g74790v3 664 BRADI_1g09890v3 0 BRADI_1g77505v3 226 BRADI_1g48960v3 0 ERR11006599 completed mapping pipeline successfully