Starting /dee2/code/volunteer_pipeline.sh ERR11006600 current disk space = 1550095097856 free memory = 1596943780 ERR11006600 SRAfilesize 4ffc675b9dc548a1a3e7101c651d45c6 ERR11006600.sra ERR11006600.sra file validated ERR11006600 is paired end ERR11006600 is conventional basespace ERR11006600 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006600_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.3315 37.0 37.0 37.0 37.0 37.0 2 36.181 37.0 37.0 37.0 37.0 37.0 3 36.3345 37.0 37.0 37.0 37.0 37.0 4 36.405 37.0 37.0 37.0 37.0 37.0 5 36.43 37.0 37.0 37.0 37.0 37.0 6 36.433 37.0 37.0 37.0 37.0 37.0 7 36.368 37.0 37.0 37.0 37.0 37.0 8 36.327 37.0 37.0 37.0 37.0 37.0 9 36.4355 37.0 37.0 37.0 37.0 37.0 10-14 36.4833 37.0 37.0 37.0 37.0 37.0 15-19 36.428399999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.3954 37.0 37.0 37.0 37.0 37.0 25-29 36.3269 37.0 37.0 37.0 37.0 37.0 30-34 36.337500000000006 37.0 37.0 37.0 37.0 37.0 35-39 36.2971 37.0 37.0 37.0 37.0 37.0 40-44 36.242900000000006 37.0 37.0 37.0 37.0 37.0 45-49 36.2724 37.0 37.0 37.0 37.0 37.0 50-54 36.2155 37.0 37.0 37.0 37.0 37.0 55-59 36.196600000000004 37.0 37.0 37.0 37.0 37.0 60-64 36.1767 37.0 37.0 37.0 37.0 37.0 65-69 36.1622 37.0 37.0 37.0 37.0 37.0 70-74 36.060700000000004 37.0 37.0 37.0 37.0 37.0 75-79 36.039100000000005 37.0 37.0 37.0 37.0 37.0 80-84 36.015699999999995 37.0 37.0 37.0 37.0 37.0 85-89 35.9996 37.0 37.0 37.0 37.0 37.0 90-94 35.930600000000005 37.0 37.0 37.0 37.0 37.0 95-99 35.961 37.0 37.0 37.0 37.0 37.0 100-104 35.9063 37.0 37.0 37.0 37.0 37.0 105-109 35.923 37.0 37.0 37.0 37.0 37.0 110-114 35.706999999999994 37.0 37.0 37.0 37.0 37.0 115-119 35.8131 37.0 37.0 37.0 37.0 37.0 120-124 35.800599999999996 37.0 37.0 37.0 37.0 37.0 125-129 35.7023 37.0 37.0 37.0 37.0 37.0 130-134 35.55 37.0 37.0 37.0 37.0 37.0 135-139 35.499100000000006 37.0 37.0 37.0 37.0 37.0 140-144 35.3057 37.0 37.0 37.0 34.6 37.0 145-149 35.430400000000006 37.0 37.0 37.0 37.0 37.0 150 35.5225 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 1.0 22 0.0 23 0.0 24 2.0 25 6.0 26 12.0 27 14.0 28 22.0 29 37.0 30 41.0 31 55.0 32 79.0 33 94.0 34 161.0 35 345.0 36 2782.0 37 348.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.05 11.825 16.075 35.05 2 28.525 11.825 26.85 32.800000000000004 3 24.825 13.425 26.775 34.975 4 27.825 15.075 24.4 32.7 5 29.975 20.875 25.025 24.125 6 26.474999999999998 27.525 20.7 25.3 7 17.75 30.725 31.724999999999998 19.8 8 18.05 30.625000000000004 30.575000000000003 20.75 9 18.75 27.275 33.900000000000006 20.075000000000003 10-14 20.985 32.005 25.564999999999998 21.445 15-19 21.224999999999998 32.61 23.805 22.36 20-24 20.69 28.73 26.815 23.765 25-29 22.650000000000002 31.215 24.12 22.015 30-34 23.815 30.23 24.25 21.705 35-39 23.064999999999998 30.695 24.065 22.175 40-44 21.0 29.86 25.195 23.945 45-49 20.825 29.360000000000003 26.545 23.27 50-54 21.62 31.380000000000003 24.775 22.225 55-59 22.91 29.45 22.869999999999997 24.77 60-64 20.945 30.43 24.91 23.715 65-69 22.225 29.48 24.305 23.990000000000002 70-74 23.205000000000002 28.945 22.58 25.27 75-79 23.275000000000002 29.095 24.185000000000002 23.445 80-84 23.365 28.78 23.78 24.075 85-89 24.03 28.275 24.945 22.75 90-94 22.43 29.485 24.89 23.195 95-99 24.245 28.935 22.89 23.93 100-104 22.545 29.73 23.72 24.005000000000003 105-109 22.97 28.335 25.040000000000003 23.655 110-114 24.044999999999998 27.605 24.58 23.77 115-119 21.345 29.87 24.69 24.095 120-124 20.74 30.075000000000003 23.65 25.535000000000004 125-129 21.39 30.580000000000002 22.275 25.755 130-134 23.119999999999997 29.475 24.654999999999998 22.75 135-139 24.279999999999998 28.76 22.509999999999998 24.45 140-144 24.905 28.26 24.46 22.375 145-149 23.905 29.37 23.945 22.78 150 23.825 26.450000000000003 25.900000000000002 23.825 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.5 21 1.0 22 0.5 23 0.5 24 1.5 25 1.5 26 3.0 27 5.5 28 3.0 29 7.5 30 17.5 31 15.5 32 16.0 33 30.0 34 40.0 35 40.5 36 71.0 37 154.0 38 240.5 39 240.0 40 237.5 41 283.0 42 262.5 43 232.5 44 213.0 45 201.5 46 196.0 47 170.5 48 144.5 49 110.0 50 84.0 51 76.5 52 69.0 53 52.5 54 45.5 55 38.5 56 33.5 57 33.0 58 34.5 59 37.0 60 39.5 61 35.0 62 26.0 63 27.0 64 44.0 65 61.0 66 43.5 67 26.0 68 30.5 69 31.5 70 32.0 71 29.0 72 20.0 73 19.5 74 19.0 75 15.0 76 11.5 77 8.5 78 7.0 79 7.5 80 7.0 81 5.0 82 4.5 83 2.0 84 0.0 85 0.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.725 #Duplication Level Percentage of deduplicated Percentage of total 1 79.80517047583365 53.25 2 13.63806669164481 18.2 3 2.547770700636943 5.1 4 1.1240164855751216 3.0 5 0.6369426751592357 2.125 6 0.5245410266017235 2.1 7 0.3372049456725365 1.575 8 0.22480329711502436 1.2 9 0.14986886474334957 0.8999999999999999 >10 0.9741476208317721 10.45 >50 0.03746721618583739 2.1 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 84 2.1 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 36 0.8999999999999999 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 27 0.675 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 26 0.65 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 21 0.525 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 21 0.525 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 20 0.5 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 18 0.44999999999999996 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 17 0.42500000000000004 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 17 0.42500000000000004 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 17 0.42500000000000004 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 16 0.4 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 16 0.4 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 14 0.35000000000000003 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 14 0.35000000000000003 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 14 0.35000000000000003 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 13 0.325 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 13 0.325 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 12 0.3 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 12 0.3 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 12 0.3 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 11 0.27499999999999997 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 11 0.27499999999999997 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 10 0.25 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 10 0.25 No Hit GAGCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTA 10 0.25 No Hit CAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTG 10 0.25 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 9 0.22499999999999998 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 9 0.22499999999999998 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 9 0.22499999999999998 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 9 0.22499999999999998 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 8 0.2 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 8 0.2 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 8 0.2 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 8 0.2 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 8 0.2 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 8 0.2 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 7 0.17500000000000002 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 7 0.17500000000000002 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 7 0.17500000000000002 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 7 0.17500000000000002 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 7 0.17500000000000002 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 7 0.17500000000000002 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 7 0.17500000000000002 No Hit CACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCA 7 0.17500000000000002 No Hit GCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATC 7 0.17500000000000002 No Hit CACCAGCTACACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCT 6 0.15 No Hit GTTGAACGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTA 6 0.15 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 6 0.15 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 6 0.15 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 6 0.15 No Hit ACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 6 0.15 No Hit TGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGA 6 0.15 No Hit ATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATA 6 0.15 No Hit CGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAAC 6 0.15 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 6 0.15 No Hit ACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACT 6 0.15 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 6 0.15 No Hit TAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGA 6 0.15 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 6 0.15 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 5 0.125 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 5 0.125 No Hit GGGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACA 5 0.125 No Hit CGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 5 0.125 No Hit GTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGC 5 0.125 No Hit TGGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAA 5 0.125 No Hit GCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCC 5 0.125 No Hit GCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGAT 5 0.125 No Hit GCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATT 5 0.125 No Hit GAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATG 5 0.125 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 5 0.125 No Hit CCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAA 5 0.125 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 5 0.125 No Hit AGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 5 0.125 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 5 0.125 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 5 0.125 No Hit GGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.025 0.0 0.0 0.0 0.0 108-109 0.037500000000000006 0.0 0.0 0.0 0.0 110-111 0.05 0.0 0.0 0.0 0.0 112-113 0.05 0.0 0.0 0.0 0.0 114-115 0.05 0.0 0.0 0.0 0.0 116-117 0.0625 0.0 0.0 0.0 0.0 118-119 0.1 0.0 0.0 0.0 0.0 120-121 0.1 0.0 0.0 0.0 0.0 122-123 0.125 0.0 0.0 0.0 0.0 124-125 0.125 0.0 0.0 0.0 0.0 126-127 0.175 0.0 0.0 0.0 0.0 128-129 0.175 0.0 0.0 0.0 0.0 130-131 0.225 0.0 0.0 0.0 0.0 132-133 0.25 0.0 0.0 0.0 0.0 134-135 0.275 0.0 0.0 0.0 0.0 136-137 0.275 0.0 0.0 0.0 0.0 138 0.3 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTCTAG 10 0.006973645 144.0 8 TCTTTTC 30 0.0018473949 72.0 5 GCTTTCT 30 0.0018473949 72.0 1 CTTTTCT 35 0.0034045284 61.714283 6 TTTCTTC 35 0.0034045284 61.714283 8 TTTCTTT 40 0.005777437 54.0 3 TTCTTTT 40 0.005777437 54.0 4 TTCTTCA 40 0.005777437 54.0 9 AAAATAA 40 0.005777437 54.0 6 GCCAAAA 40 0.005777437 54.0 3 CAAAATA 40 0.005777437 54.0 5 TTTTCTT 45 0.009205684 48.0 7 CTTTCTT 45 0.009205684 48.0 2 TAGCGGA 30 0.0015031899 23.999998 30-34 TATGTTA 30 0.0015031899 23.999998 25-29 TGTTAGC 30 0.0015031899 23.999998 25-29 AGCGGAA 30 0.0015031899 23.999998 30-34 ATGTTAG 30 0.0015031899 23.999998 25-29 AAAATTC 30 0.0015031899 23.999998 15-19 TTAGCGG 30 0.0015031899 23.999998 30-34 >>END_MODULE ERR11006600 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006600_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.37775 37.0 37.0 37.0 37.0 37.0 2 36.25 37.0 37.0 37.0 37.0 37.0 3 36.258 37.0 37.0 37.0 37.0 37.0 4 36.3425 37.0 37.0 37.0 37.0 37.0 5 36.399 37.0 37.0 37.0 37.0 37.0 6 36.235 37.0 37.0 37.0 37.0 37.0 7 36.2815 37.0 37.0 37.0 37.0 37.0 8 36.3425 37.0 37.0 37.0 37.0 37.0 9 36.376 37.0 37.0 37.0 37.0 37.0 10-14 36.3021 37.0 37.0 37.0 37.0 37.0 15-19 36.319100000000006 37.0 37.0 37.0 37.0 37.0 20-24 36.2326 37.0 37.0 37.0 37.0 37.0 25-29 36.24209999999999 37.0 37.0 37.0 37.0 37.0 30-34 36.1799 37.0 37.0 37.0 37.0 37.0 35-39 36.1168 37.0 37.0 37.0 37.0 37.0 40-44 36.1152 37.0 37.0 37.0 37.0 37.0 45-49 36.12220000000001 37.0 37.0 37.0 37.0 37.0 50-54 36.0949 37.0 37.0 37.0 37.0 37.0 55-59 36.0142 37.0 37.0 37.0 37.0 37.0 60-64 35.960899999999995 37.0 37.0 37.0 37.0 37.0 65-69 35.999 37.0 37.0 37.0 37.0 37.0 70-74 35.9251 37.0 37.0 37.0 37.0 37.0 75-79 35.939 37.0 37.0 37.0 37.0 37.0 80-84 35.8932 37.0 37.0 37.0 37.0 37.0 85-89 35.769400000000005 37.0 37.0 37.0 37.0 37.0 90-94 35.826 37.0 37.0 37.0 37.0 37.0 95-99 35.6853 37.0 37.0 37.0 37.0 37.0 100-104 35.6632 37.0 37.0 37.0 37.0 37.0 105-109 35.5514 37.0 37.0 37.0 37.0 37.0 110-114 35.4862 37.0 37.0 37.0 37.0 37.0 115-119 35.4528 37.0 37.0 37.0 37.0 37.0 120-124 35.333400000000005 37.0 37.0 37.0 32.2 37.0 125-129 35.4208 37.0 37.0 37.0 37.0 37.0 130-134 35.31849999999999 37.0 37.0 37.0 32.2 37.0 135-139 35.192 37.0 37.0 37.0 27.4 37.0 140-144 35.102 37.0 37.0 37.0 25.0 37.0 145-149 35.1455 37.0 37.0 37.0 27.4 37.0 150 34.9935 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 0.0 17 1.0 18 0.0 19 2.0 20 1.0 21 3.0 22 4.0 23 7.0 24 5.0 25 4.0 26 10.0 27 18.0 28 24.0 29 34.0 30 39.0 31 49.0 32 69.0 33 124.0 34 205.0 35 498.0 36 2667.0 37 235.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.91165172855314 24.37900128040973 17.157490396927017 29.551856594110117 2 26.875 25.124999999999996 30.9 17.1 3 21.175 27.224999999999998 31.2 20.4 4 24.4 27.6 27.125 20.875 5 24.975 27.825 27.1 20.1 6 22.225 31.324999999999996 27.025 19.425 7 21.95 20.474999999999998 38.475 19.1 8 22.650000000000002 21.325 28.625 27.400000000000002 9 22.825 20.599999999999998 34.0 22.575 10-14 23.995 25.345000000000002 27.58 23.080000000000002 15-19 24.8 24.240000000000002 29.5 21.46 20-24 25.22 23.9 28.735 22.145 25-29 24.975 24.19 28.84 21.995 30-34 25.465 24.115000000000002 28.425 21.995 35-39 25.21 23.355 28.720000000000002 22.715 40-44 23.775 25.064999999999998 28.83 22.33 45-49 24.345 25.635 28.449999999999996 21.57 50-54 23.665 25.240000000000002 27.965 23.13 55-59 24.62 24.395 27.515 23.47 60-64 23.61 24.08 28.945 23.365 65-69 24.285 24.205 28.59 22.919999999999998 70-74 24.54 23.974999999999998 29.375 22.11 75-79 24.82 24.0 29.330000000000002 21.85 80-84 25.355 24.349999999999998 28.970000000000002 21.325 85-89 25.395 24.285 27.485 22.835 90-94 24.925 24.779999999999998 27.705000000000002 22.59 95-99 24.43 24.435000000000002 28.54 22.595000000000002 100-104 24.94 23.275000000000002 29.235 22.55 105-109 26.39 22.73 28.67 22.21 110-114 24.42 23.72 28.775000000000002 23.085 115-119 25.44 24.13 28.050000000000004 22.38 120-124 24.39 24.515 28.199999999999996 22.895 125-129 23.275000000000002 24.285 29.110000000000003 23.330000000000002 130-134 23.605 25.419999999999998 28.384999999999998 22.59 135-139 24.985 24.15 28.95 21.915000000000003 140-144 23.57 24.555 29.494999999999997 22.38 145-149 23.54 23.835 30.11 22.515 150 24.625 24.3 27.474999999999998 23.599999999999998 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.5 19 1.0 20 0.5 21 1.0 22 2.5 23 3.0 24 4.0 25 11.0 26 12.5 27 11.0 28 9.0 29 9.5 30 17.5 31 19.5 32 24.0 33 26.5 34 37.0 35 59.0 36 93.0 37 156.0 38 181.0 39 172.0 40 210.5 41 244.5 42 229.5 43 230.5 44 249.0 45 233.5 46 197.0 47 150.0 48 121.5 49 103.0 50 72.0 51 64.0 52 64.5 53 58.0 54 46.5 55 45.5 56 43.5 57 38.5 58 42.5 59 39.0 60 39.0 61 36.5 62 27.0 63 37.5 64 66.0 65 66.0 66 49.0 67 44.5 68 41.5 69 35.0 70 30.0 71 30.5 72 28.0 73 21.0 74 19.0 75 21.0 76 16.5 77 13.0 78 13.5 79 9.5 80 7.0 81 6.0 82 3.5 83 2.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.375 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.825 #Duplication Level Percentage of deduplicated Percentage of total 1 79.77085571070533 55.7 2 12.85356247762263 17.95 3 3.0433225921947726 6.375 4 1.611170784103115 4.5 5 0.895094880057286 3.125 6 0.4654493376297888 1.95 7 0.2864303616183316 1.4000000000000001 8 0.322234156820623 1.7999999999999998 9 0.21482277121374865 1.35 >10 0.5370569280343717 5.8500000000000005 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 44 1.0999999999999999 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 21 0.525 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 20 0.5 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 18 0.44999999999999996 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 17 0.42500000000000004 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 15 0.375 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 14 0.35000000000000003 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 12 0.3 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 12 0.3 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 11 0.27499999999999997 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 10 0.25 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 10 0.25 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 10 0.25 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 10 0.25 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 10 0.25 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 9 0.22499999999999998 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 9 0.22499999999999998 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 9 0.22499999999999998 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 9 0.22499999999999998 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 9 0.22499999999999998 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 9 0.22499999999999998 No Hit GGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTC 8 0.2 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 8 0.2 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 8 0.2 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 8 0.2 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 8 0.2 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 8 0.2 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 8 0.2 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 8 0.2 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 8 0.2 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 7 0.17500000000000002 No Hit GTATAAACGAATTTTTTTTATTTTCTTAGACTTAGACCCTGCAAGATAAT 7 0.17500000000000002 No Hit TTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGG 7 0.17500000000000002 No Hit GAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCG 7 0.17500000000000002 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 7 0.17500000000000002 No Hit TCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTG 7 0.17500000000000002 No Hit CTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTC 7 0.17500000000000002 No Hit GAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGT 7 0.17500000000000002 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 6 0.15 No Hit ATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCT 6 0.15 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 6 0.15 No Hit ATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCAC 6 0.15 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 6 0.15 No Hit CTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAG 6 0.15 No Hit ACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGA 6 0.15 No Hit CTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAG 6 0.15 No Hit CTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGG 6 0.15 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 6 0.15 No Hit TAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAG 6 0.15 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 6 0.15 No Hit ATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTG 6 0.15 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 5 0.125 No Hit GAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGAC 5 0.125 No Hit GTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTC 5 0.125 No Hit GAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTT 5 0.125 No Hit TGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGA 5 0.125 No Hit CCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGG 5 0.125 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 5 0.125 No Hit CAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGT 5 0.125 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 5 0.125 No Hit GATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTC 5 0.125 No Hit AGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAG 5 0.125 No Hit AACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGG 5 0.125 No Hit GTAGCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGT 5 0.125 No Hit GGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTT 5 0.125 No Hit ATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGT 5 0.125 No Hit TTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAAC 5 0.125 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 5 0.125 No Hit TATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTAT 5 0.125 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 5 0.125 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 5 0.125 No Hit AACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGG 5 0.125 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 5 0.125 No Hit GTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAAT 5 0.125 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 5 0.125 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.025 0.0 0.0 0.0 0.0 108-109 0.037500000000000006 0.0 0.0 0.0 0.0 110-111 0.05 0.0 0.0 0.0 0.0 112-113 0.05 0.0 0.0 0.0 0.0 114-115 0.05 0.0 0.0 0.0 0.0 116-117 0.0625 0.0 0.0 0.0 0.0 118-119 0.1 0.0 0.0 0.0 0.0 120-121 0.1 0.0 0.0 0.0 0.0 122-123 0.1375 0.0 0.0 0.0 0.0 124-125 0.15 0.0 0.0 0.0 0.0 126-127 0.2 0.0 0.0 0.0 0.0 128-129 0.2 0.0 0.0 0.0 0.0 130-131 0.25 0.0 0.0 0.0 0.0 132-133 0.275 0.0 0.0 0.0 0.0 134-135 0.3 0.0 0.0 0.0 0.0 136-137 0.3 0.0 0.0 0.0 0.0 138 0.325 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 3786522 spots for ERR11006600.sra Written 3786522 spots for ERR11006600.sra Read 3786522 spots for ERR11006600.sra Written 3786522 spots for ERR11006600.sra Read 3786522 spots for ERR11006600.sra Written 3786522 spots for ERR11006600.sra Read 3786522 spots for ERR11006600.sra Written 3786522 spots for ERR11006600.sra Read 3786522 spots for ERR11006600.sra Written 3786522 spots for ERR11006600.sra Read 3786522 spots for ERR11006600.sra Written 3786522 spots for ERR11006600.sra Read 3786522 spots for ERR11006600.sra Written 3786522 spots for ERR11006600.sra Read 3786522 spots for ERR11006600.sra Written 3786522 spots for ERR11006600.sra Read 3786522 spots for ERR11006600.sra Written 3786522 spots for ERR11006600.sra Read 3786522 spots for ERR11006600.sra Written 3786522 spots for ERR11006600.sra Read 3786522 spots for ERR11006600.sra Written 3786522 spots for ERR11006600.sra Read 3786522 spots for ERR11006600.sra Written 3786522 spots for ERR11006600.sra Read 3786522 spots for ERR11006600.sra Written 3786522 spots for ERR11006600.sra Read 3786522 spots for ERR11006600.sra Written 3786522 spots for ERR11006600.sra Read 3786524 spots for ERR11006600.sra Written 3786524 spots for ERR11006600.sra Read 3786522 spots for ERR11006600.sra Written 3786522 spots for ERR11006600.sra Read 3786522 spots for ERR11006600.sra Written 3786522 spots for ERR11006600.sra Read 3786522 spots for ERR11006600.sra Written 3786522 spots for ERR11006600.sra Read 3786522 spots for ERR11006600.sra Written 3786522 spots for ERR11006600.sra Read 3786522 spots for ERR11006600.sra Written 3786522 spots for ERR11006600.sra SRR ids: ['ERR11006600.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_91e4wf6i ERR11006600.sra spots: 75730442 blocks: [[1, 3786522], [3786523, 7573044], [7573045, 11359566], [11359567, 15146088], [15146089, 18932610], [18932611, 22719132], [22719133, 26505654], [26505655, 30292176], [30292177, 34078698], [34078699, 37865220], [37865221, 41651742], [41651743, 45438264], [45438265, 49224786], [49224787, 53011308], [53011309, 56797830], [56797831, 60584352], [60584353, 64370874], [64370875, 68157396], [68157397, 71943918], [71943919, 75730442]] ERR11006600 file size 27830901 ERR11006600 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006600 ERR11006600_1.fastq ERR11006600_2.fastq Input file: ERR11006600_1.fastq Paired file: ERR11006600_2.fastq trimmed: ERR11006600-trimmed-pair1.fastq, ERR11006600-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 19:12:43 2024 >> started Fri Dec 6 19:14:15 2024 >> done (92.134s) 75730442 read pairs processed; of these: 436 ( 0.00%) short read pairs filtered out after trimming by size control 8762 ( 0.01%) empty read pairs filtered out after trimming by size control 75721244 (99.99%) read pairs available; of these: 589833 ( 0.78%) trimmed read pairs available after processing 75131411 (99.22%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 15 0.00% 19 20 0.00% 20 23 0.00% 21 23 0.00% 22 33 0.00% 23 26 0.00% 24 14 0.00% 25 37 0.00% 26 25 0.00% 27 36 0.00% 28 25 0.00% 29 64 0.00% 30 24 0.00% 31 128 0.00% 32 29 0.00% 33 32 0.00% 34 23 0.00% 35 33 0.00% 36 31 0.00% 37 31 0.00% 38 28 0.00% 39 42 0.00% 40 42 0.00% 41 62 0.00% 42 40 0.00% 43 40 0.00% 44 60 0.00% 45 57 0.00% 46 62 0.00% 47 65 0.00% 48 66 0.00% 49 68 0.00% 50 82 0.00% 51 104 0.00% 52 102 0.00% 53 123 0.00% 54 120 0.00% 55 136 0.00% 56 130 0.00% 57 152 0.00% 58 141 0.00% 59 185 0.00% 60 161 0.00% 61 247 0.00% 62 225 0.00% 63 220 0.00% 64 267 0.00% 65 272 0.00% 66 308 0.00% 67 309 0.00% 68 362 0.00% 69 385 0.00% 70 407 0.00% 71 399 0.00% 72 465 0.00% 73 485 0.00% 74 575 0.00% 75 614 0.00% 76 612 0.00% 77 640 0.00% 78 699 0.00% 79 766 0.00% 80 785 0.00% 81 821 0.00% 82 881 0.00% 83 978 0.00% 84 958 0.00% 85 1144 0.00% 86 1347 0.00% 87 1329 0.00% 88 1333 0.00% 89 1460 0.00% 90 1557 0.00% 91 1561 0.00% 92 1730 0.00% 93 1797 0.00% 94 1790 0.00% 95 2076 0.00% 96 2217 0.00% 97 2312 0.00% 98 2492 0.00% 99 2622 0.00% 100 2757 0.00% 101 2866 0.00% 102 2905 0.00% 103 3203 0.00% 104 3258 0.00% 105 3708 0.00% 106 3732 0.00% 107 3916 0.01% 108 4079 0.01% 109 4514 0.01% 110 4624 0.01% 111 4876 0.01% 112 5173 0.01% 113 5187 0.01% 114 5676 0.01% 115 5738 0.01% 116 6418 0.01% 117 6645 0.01% 118 6702 0.01% 119 7378 0.01% 120 7820 0.01% 121 7929 0.01% 122 8603 0.01% 123 8766 0.01% 124 9347 0.01% 125 10484 0.01% 126 9850 0.01% 127 9514 0.01% 128 10218 0.01% 129 11111 0.01% 130 12198 0.02% 131 12651 0.02% 132 13429 0.02% 133 12928 0.02% 134 13879 0.02% 135 13760 0.02% 136 14682 0.02% 137 14980 0.02% 138 15482 0.02% 139 16654 0.02% 140 17174 0.02% 141 18827 0.02% 142 19380 0.03% 143 20447 0.03% 144 20996 0.03% 145 22109 0.03% 146 24583 0.03% 147 24366 0.03% 148 25332 0.03% 149 26792 0.04% 150 75131411 99.22% 75721244 reads passed initial QC criterion=sequence-density sequence-density=0.29 sequence-density-rank=1 fanout-score=0.00 fanout-score-rank=32 prefix-density=0.00 prefix-fanout=1.0 sequence=TGCGGGAACTTC criterion=fanout-score sequence-density=0.06 sequence-density-rank=20 fanout-score=34.81 fanout-score-rank=1 prefix-density=1.97 prefix-fanout=1.1 sequence=CAGCCTCACGCGGTGCCT criterion=sequence-density sequence-density=0.21 sequence-density-rank=1 fanout-score=9.98 fanout-score-rank=9 prefix-density=2.07 prefix-fanout=1.0 sequence=TTGCGTAGTGGATCTGCTGGGGCCTATGCGAAAGCTGGGCCTCACGAATTCTATAGTGGCAGGCACCGCGTTAGGCTGGCTTC criterion=fanout-score sequence-density=0.06 sequence-density-rank=24 fanout-score=144.58 fanout-score-rank=1 prefix-density=8.89 prefix-fanout=1.0 sequence=ATAACTAGCACAGAAAATCGTCT ERR11006600 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 19:14:56 Started mapping on | Dec 06 19:14:57 Finished on | Dec 06 19:22:57 Mapping speed, Million of reads per hour | 567.91 Number of input reads | 75721244 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 62762986 Uniquely mapped reads % | 82.89% Average mapped length | 298.49 Number of splices: Total | 27066094 Number of splices: Annotated (sjdb) | 25363451 Number of splices: GT/AG | 26649861 Number of splices: GC/AG | 285041 Number of splices: AT/AC | 12755 Number of splices: Non-canonical | 118437 Mismatch rate per base, % | 0.28% Deletion rate per base | 0.02% Deletion average length | 1.91 Insertion rate per base | 0.01% Insertion average length | 2.20 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 10292774 % of reads mapped to multiple loci | 13.59% Number of reads mapped to too many loci | 22102 % of reads mapped to too many loci | 0.03% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.52% % of reads unmapped: other | 0.97% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2665484 2665484 2665484 N_multimapping 10292774 10292774 10292774 N_noFeature 13934504 57782341 17757745 N_ambiguous 1614878 70922 417907 UnstrandedReadsAssigned:47213604 PositiveStrandReadsAssigned:4909723 NegativeStrandReadsAssigned:44587334 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006600 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006600-trimmed-pair1.fastq ERR11006600-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 75,721,244 reads, 46,269,326 reads pseudoaligned [quant] estimated average fragment length: 272.839 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,198 rounds 52973 ERR11006600.ke.tsv 35125 ERR11006600.se.tsv 88098 total ==> ERR11006600.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 664.497 0 0 PNS24247 1044 772.161 53.0362 1.67558 PNS24249 1928 1656.16 463.926 6.83356 PNS24246 1044 772.161 53.0362 1.67558 PNS24248 1044 772.161 53.0362 1.67558 PNS24244 1471 1199.16 141.965 2.88805 PNS24243 293 51.7195 4 1.88671 KQK14069 1603 1331.16 751.416 13.7705 KQK14071 474 205.225 3.64063 0.43276 ==> ERR11006600.se.tsv <== BRADI_1g14170v3 813 BRADI_1g53295v3 1209 BRADI_1g59795v3 360 BRADI_1g07683v3 0 BRADI_1g00485v3 17 BRADI_1g20270v3 1095 BRADI_1g74790v3 1361 BRADI_1g09890v3 1 BRADI_1g77505v3 188 BRADI_1g48960v3 0 ERR11006600 completed mapping pipeline successfully