Starting /dee2/code/volunteer_pipeline.sh ERR11006601 current disk space = 1549887430656 free memory = 1597307076 ERR11006601 SRAfilesize 674946abac8b4dfa98fb299156179922 ERR11006601.sra ERR11006601.sra file validated ERR11006601 is paired end ERR11006601 is conventional basespace ERR11006601 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006601_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.42725 37.0 37.0 37.0 37.0 37.0 2 36.4985 37.0 37.0 37.0 37.0 37.0 3 36.525 37.0 37.0 37.0 37.0 37.0 4 36.5415 37.0 37.0 37.0 37.0 37.0 5 36.527 37.0 37.0 37.0 37.0 37.0 6 36.5805 37.0 37.0 37.0 37.0 37.0 7 36.539 37.0 37.0 37.0 37.0 37.0 8 36.48 37.0 37.0 37.0 37.0 37.0 9 36.453 37.0 37.0 37.0 37.0 37.0 10-14 36.406600000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.5124 37.0 37.0 37.0 37.0 37.0 20-24 36.473200000000006 37.0 37.0 37.0 37.0 37.0 25-29 36.413799999999995 37.0 37.0 37.0 37.0 37.0 30-34 36.3293 37.0 37.0 37.0 37.0 37.0 35-39 36.3328 37.0 37.0 37.0 37.0 37.0 40-44 36.3144 37.0 37.0 37.0 37.0 37.0 45-49 36.264199999999995 37.0 37.0 37.0 37.0 37.0 50-54 36.2703 37.0 37.0 37.0 37.0 37.0 55-59 36.281 37.0 37.0 37.0 37.0 37.0 60-64 36.233700000000006 37.0 37.0 37.0 37.0 37.0 65-69 36.1693 37.0 37.0 37.0 37.0 37.0 70-74 36.1648 37.0 37.0 37.0 37.0 37.0 75-79 36.151300000000006 37.0 37.0 37.0 37.0 37.0 80-84 36.1279 37.0 37.0 37.0 37.0 37.0 85-89 36.0475 37.0 37.0 37.0 37.0 37.0 90-94 36.0766 37.0 37.0 37.0 37.0 37.0 95-99 35.9942 37.0 37.0 37.0 37.0 37.0 100-104 35.9954 37.0 37.0 37.0 37.0 37.0 105-109 35.9327 37.0 37.0 37.0 37.0 37.0 110-114 35.9413 37.0 37.0 37.0 37.0 37.0 115-119 35.846 37.0 37.0 37.0 37.0 37.0 120-124 35.799099999999996 37.0 37.0 37.0 37.0 37.0 125-129 35.726 37.0 37.0 37.0 37.0 37.0 130-134 35.68300000000001 37.0 37.0 37.0 37.0 37.0 135-139 35.567499999999995 37.0 37.0 37.0 37.0 37.0 140-144 35.46840000000001 37.0 37.0 37.0 37.0 37.0 145-149 35.5613 37.0 37.0 37.0 37.0 37.0 150 35.3985 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 2.0 22 0.0 23 3.0 24 2.0 25 2.0 26 5.0 27 5.0 28 28.0 29 19.0 30 33.0 31 55.0 32 64.0 33 78.0 34 158.0 35 339.0 36 2968.0 37 239.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.152864648486364 14.36077057793345 14.610958218663997 33.87540655491619 2 29.799999999999997 13.825000000000001 27.425 28.95 3 23.65 15.85 26.05 34.449999999999996 4 28.95 18.35 23.025000000000002 29.675 5 29.45 23.974999999999998 23.025000000000002 23.549999999999997 6 27.575 29.95 19.650000000000002 22.825 7 15.6 31.55 33.15 19.7 8 16.2 30.4 31.574999999999996 21.825 9 17.474999999999998 28.799999999999997 33.275 20.45 10-14 19.98 31.919999999999998 26.43 21.67 15-19 21.125 32.4 24.69 21.785 20-24 20.325 29.39 27.42 22.865 25-29 21.905 30.79 25.105 22.2 30-34 23.005 30.654999999999998 24.365000000000002 21.975 35-39 21.73 31.840000000000003 24.4 22.03 40-44 19.814999999999998 31.03 25.669999999999998 23.485 45-49 20.525 29.799999999999997 26.6 23.075000000000003 50-54 20.794999999999998 32.055 24.66 22.49 55-59 22.35 29.470000000000002 24.2 23.98 60-64 20.580000000000002 30.620000000000005 25.765 23.035 65-69 21.310000000000002 30.18 24.75 23.76 70-74 21.82 29.875 23.895 24.41 75-79 21.8 30.61 24.97 22.62 80-84 22.215 30.19 24.32 23.275000000000002 85-89 22.835 29.395 24.855 22.915 90-94 21.975 30.14 24.83 23.055 95-99 22.869999999999997 29.335 24.725 23.07 100-104 21.959999999999997 30.89 24.14 23.01 105-109 21.705 29.215000000000003 25.724999999999998 23.355 110-114 22.705000000000002 28.244999999999997 25.55 23.5 115-119 21.32 30.285 24.85 23.544999999999998 120-124 21.11 29.595 24.815 24.48 125-129 21.560000000000002 29.815 23.615 25.009999999999998 130-134 21.490000000000002 30.930000000000003 25.074999999999996 22.505 135-139 23.674999999999997 29.81 23.330000000000002 23.185 140-144 23.525 29.82 24.65 22.005 145-149 23.235 30.425 24.285 22.055 150 22.625 28.875 25.025 23.474999999999998 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 2.0 21 3.5 22 2.5 23 1.5 24 1.0 25 2.5 26 5.5 27 8.0 28 13.0 29 18.5 30 20.0 31 22.0 32 31.5 33 39.0 34 40.5 35 52.5 36 99.0 37 172.5 38 232.0 39 231.0 40 256.0 41 298.0 42 259.0 43 235.0 44 226.0 45 216.0 46 202.5 47 168.0 48 145.0 49 113.0 50 85.5 51 64.5 52 47.5 53 46.5 54 49.5 55 45.0 56 36.5 57 29.0 58 21.5 59 25.0 60 32.5 61 28.0 62 26.5 63 26.5 64 33.5 65 44.5 66 38.5 67 30.5 68 25.5 69 20.5 70 15.0 71 13.0 72 16.5 73 18.5 74 14.0 75 12.0 76 10.0 77 8.0 78 6.0 79 3.5 80 2.5 81 1.5 82 1.0 83 2.0 84 2.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.075 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.275 #Duplication Level Percentage of deduplicated Percentage of total 1 82.07646439845668 58.5 2 11.539810592774465 16.45 3 2.981410031567871 6.375 4 0.8768853034023149 2.5 5 0.5612065941774815 2.0 6 0.7365836548579445 3.15 7 0.2104524728165556 1.05 8 0.2455278849526482 1.4000000000000001 9 0.175377060680463 1.125 >10 0.5612065941774815 5.925 >50 0.035075412136092596 1.525 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 61 1.525 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 25 0.625 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 24 0.6 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 18 0.44999999999999996 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 17 0.42500000000000004 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 16 0.4 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 16 0.4 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 15 0.375 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 13 0.325 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 13 0.325 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 12 0.3 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 12 0.3 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 12 0.3 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 12 0.3 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 11 0.27499999999999997 No Hit GGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGG 11 0.27499999999999997 No Hit TCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGT 10 0.25 No Hit GGGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACA 9 0.22499999999999998 No Hit GACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGG 9 0.22499999999999998 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 9 0.22499999999999998 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 9 0.22499999999999998 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 9 0.22499999999999998 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 8 0.2 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 8 0.2 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 8 0.2 No Hit CACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCA 8 0.2 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 8 0.2 No Hit CATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAA 8 0.2 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 8 0.2 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 7 0.17500000000000002 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 7 0.17500000000000002 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 7 0.17500000000000002 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 7 0.17500000000000002 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 7 0.17500000000000002 No Hit TAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGA 7 0.17500000000000002 No Hit CTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAAT 6 0.15 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 6 0.15 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 6 0.15 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 6 0.15 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 6 0.15 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 6 0.15 No Hit CAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAGGG 6 0.15 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 6 0.15 No Hit TTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAGC 6 0.15 No Hit GCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCC 6 0.15 No Hit TGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGA 6 0.15 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 6 0.15 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 6 0.15 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 6 0.15 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 6 0.15 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 6 0.15 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 6 0.15 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 6 0.15 No Hit ATTGGGTATTTCTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGG 6 0.15 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 6 0.15 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 6 0.15 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 5 0.125 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 5 0.125 No Hit GGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAA 5 0.125 No Hit GGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTA 5 0.125 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 5 0.125 No Hit ATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTT 5 0.125 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 5 0.125 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 5 0.125 No Hit GATTGGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACC 5 0.125 No Hit CCATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGT 5 0.125 No Hit GTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCA 5 0.125 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 5 0.125 No Hit CAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAA 5 0.125 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 5 0.125 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 5 0.125 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.037500000000000006 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.0875 0.0 0.0 0.0 0.0 86-87 0.1125 0.0 0.0 0.0 0.0 88-89 0.1375 0.0 0.0 0.0 0.0 90-91 0.15 0.0 0.0 0.0 0.0 92-93 0.15 0.0 0.0 0.0 0.0 94-95 0.175 0.0 0.0 0.0 0.0 96-97 0.225 0.0 0.0 0.0 0.0 98-99 0.25 0.0 0.0 0.0 0.0 100-101 0.25 0.0 0.0 0.0 0.0 102-103 0.3125 0.0 0.0 0.0 0.0 104-105 0.325 0.0 0.0 0.0 0.0 106-107 0.36250000000000004 0.0 0.0 0.0 0.0 108-109 0.4875 0.0 0.0 0.0 0.0 110-111 0.6125 0.0 0.0 0.0 0.0 112-113 0.7125 0.0 0.0 0.0 0.0 114-115 0.775 0.0 0.0 0.0 0.0 116-117 0.825 0.0 0.0 0.0 0.0 118-119 0.8875 0.0 0.0 0.0 0.0 120-121 1.0 0.0 0.0 0.0 0.0 122-123 1.125 0.0 0.0 0.0 0.0 124-125 1.1875 0.0 0.0 0.0 0.0 126-127 1.2625 0.0 0.0 0.0 0.0 128-129 1.3624999999999998 0.0 0.0 0.0 0.0 130-131 1.6125 0.0 0.0 0.0 0.0 132-133 1.6875 0.0 0.0 0.0 0.0 134-135 1.875 0.0 0.0 0.0 0.0 136-137 2.0125 0.0 0.0 0.0 0.0 138 2.125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAGGCT 35 0.0036813593 20.571428 140-144 >>END_MODULE ERR11006601 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006601_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.152 37.0 37.0 37.0 37.0 37.0 2 36.0115 37.0 37.0 37.0 37.0 37.0 3 36.19 37.0 37.0 37.0 37.0 37.0 4 36.097 37.0 37.0 37.0 37.0 37.0 5 36.213 37.0 37.0 37.0 37.0 37.0 6 36.2925 37.0 37.0 37.0 37.0 37.0 7 36.1785 37.0 37.0 37.0 37.0 37.0 8 36.3495 37.0 37.0 37.0 37.0 37.0 9 36.2335 37.0 37.0 37.0 37.0 37.0 10-14 36.31079999999999 37.0 37.0 37.0 37.0 37.0 15-19 36.247 37.0 37.0 37.0 37.0 37.0 20-24 36.267999999999994 37.0 37.0 37.0 37.0 37.0 25-29 36.1608 37.0 37.0 37.0 37.0 37.0 30-34 36.1075 37.0 37.0 37.0 37.0 37.0 35-39 36.093 37.0 37.0 37.0 37.0 37.0 40-44 36.1077 37.0 37.0 37.0 37.0 37.0 45-49 36.0482 37.0 37.0 37.0 37.0 37.0 50-54 35.9664 37.0 37.0 37.0 37.0 37.0 55-59 35.856 37.0 37.0 37.0 37.0 37.0 60-64 35.8799 37.0 37.0 37.0 37.0 37.0 65-69 35.7995 37.0 37.0 37.0 37.0 37.0 70-74 35.761 37.0 37.0 37.0 37.0 37.0 75-79 35.7065 37.0 37.0 37.0 37.0 37.0 80-84 35.6585 37.0 37.0 37.0 37.0 37.0 85-89 35.6289 37.0 37.0 37.0 37.0 37.0 90-94 35.658699999999996 37.0 37.0 37.0 37.0 37.0 95-99 35.5757 37.0 37.0 37.0 34.6 37.0 100-104 35.500299999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.482899999999994 37.0 37.0 37.0 37.0 37.0 110-114 35.4812 37.0 37.0 37.0 37.0 37.0 115-119 35.3218 37.0 37.0 37.0 32.2 37.0 120-124 35.2971 37.0 37.0 37.0 32.2 37.0 125-129 35.2205 37.0 37.0 37.0 29.8 37.0 130-134 34.9557 37.0 37.0 37.0 25.0 37.0 135-139 35.047700000000006 37.0 37.0 37.0 27.4 37.0 140-144 34.923 37.0 37.0 37.0 25.0 37.0 145-149 34.756299999999996 37.0 37.0 37.0 25.0 37.0 150 34.6845 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0 20 1.0 21 0.0 22 1.0 23 6.0 24 6.0 25 10.0 26 10.0 27 13.0 28 20.0 29 25.0 30 56.0 31 69.0 32 76.0 33 145.0 34 229.0 35 703.0 36 2478.0 37 150.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 30.025000000000002 25.224999999999998 16.6 28.15 2 25.074999999999996 26.174999999999997 34.75 14.000000000000002 3 20.200000000000003 29.875 31.025000000000002 18.9 4 23.974999999999998 28.9 25.624999999999996 21.5 5 24.55 30.225 27.275 17.95 6 20.9 34.5 26.275 18.325 7 21.0 19.85 39.25 19.900000000000002 8 21.825 23.150000000000002 31.125000000000004 23.9 9 21.325 22.400000000000002 33.300000000000004 22.975 10-14 24.075 26.224999999999998 28.52 21.18 15-19 23.66 25.245 29.82 21.275 20-24 24.125 25.46 29.185 21.23 25-29 23.5 24.5 29.92 22.08 30-34 23.93 25.095 30.270000000000003 20.705000000000002 35-39 23.89 24.490000000000002 30.220000000000002 21.4 40-44 22.86 25.324999999999996 30.259999999999998 21.555 45-49 23.23 26.240000000000002 29.220000000000002 21.310000000000002 50-54 23.775 25.36 28.715000000000003 22.15 55-59 23.46 25.224999999999998 28.935 22.38 60-64 23.43 25.09 29.5 21.98 65-69 23.415 25.374999999999996 28.985 22.225 70-74 23.915 25.180000000000003 29.330000000000002 21.575 75-79 23.07 25.05 30.18 21.7 80-84 24.654999999999998 24.66 29.56 21.125 85-89 24.349999999999998 25.0 28.815 21.834999999999997 90-94 23.265 24.64 29.985 22.11 95-99 23.36 25.230000000000004 29.525000000000002 21.884999999999998 100-104 23.435 23.655 31.014999999999997 21.895 105-109 24.43 24.32 29.74 21.51 110-114 23.71 25.03 28.904999999999998 22.355 115-119 24.545 25.245 28.675 21.535 120-124 24.01 25.355 28.73 21.905 125-129 23.21 25.525 29.409999999999997 21.855 130-134 23.369999999999997 25.415 29.175 22.040000000000003 135-139 23.995 24.915000000000003 29.82 21.27 140-144 23.96 25.124999999999996 29.79 21.125 145-149 23.125 25.11 29.695 22.07 150 23.9 23.95 29.025000000000002 23.125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 3.0 21 4.0 22 1.5 23 2.5 24 3.0 25 7.5 26 12.5 27 14.5 28 16.0 29 20.0 30 30.5 31 35.0 32 35.0 33 41.0 34 52.5 35 70.5 36 109.0 37 170.0 38 196.0 39 204.0 40 230.0 41 253.0 42 221.5 43 222.5 44 279.0 45 248.5 46 195.0 47 166.5 48 126.0 49 90.5 50 85.0 51 77.5 52 57.5 53 44.5 54 39.0 55 38.5 56 38.0 57 39.5 58 38.0 59 30.5 60 28.5 61 26.5 62 23.5 63 35.5 64 37.5 65 34.5 66 30.5 67 24.5 68 22.5 69 24.5 70 28.5 71 22.0 72 15.5 73 18.5 74 19.0 75 11.0 76 7.5 77 6.5 78 6.5 79 7.0 80 8.0 81 5.5 82 1.5 83 1.5 84 2.0 85 1.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.25 #Duplication Level Percentage of deduplicated Percentage of total 1 82.11072664359862 59.325 2 11.453287197231834 16.55 3 2.5605536332179932 5.55 4 1.453287197231834 4.2 5 0.657439446366782 2.375 6 0.3806228373702422 1.6500000000000001 7 0.27681660899653976 1.4000000000000001 8 0.20761245674740486 1.2 9 0.27681660899653976 1.7999999999999998 >10 0.6228373702422145 5.949999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 22 0.5499999999999999 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 20 0.5 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 17 0.42500000000000004 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 17 0.42500000000000004 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 15 0.375 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 15 0.375 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 13 0.325 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 12 0.3 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 12 0.3 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 12 0.3 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 11 0.27499999999999997 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 11 0.27499999999999997 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 11 0.27499999999999997 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 10 0.25 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 10 0.25 No Hit CTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGT 10 0.25 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 10 0.25 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 10 0.25 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 9 0.22499999999999998 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 9 0.22499999999999998 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 9 0.22499999999999998 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 9 0.22499999999999998 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 9 0.22499999999999998 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 9 0.22499999999999998 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 9 0.22499999999999998 No Hit AGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGC 9 0.22499999999999998 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 8 0.2 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 8 0.2 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 8 0.2 No Hit CAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTC 8 0.2 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 8 0.2 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 8 0.2 No Hit GTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACC 7 0.17500000000000002 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 7 0.17500000000000002 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 7 0.17500000000000002 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 7 0.17500000000000002 No Hit AAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAA 7 0.17500000000000002 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 7 0.17500000000000002 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 7 0.17500000000000002 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 7 0.17500000000000002 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 6 0.15 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 6 0.15 No Hit GTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTATG 6 0.15 No Hit GAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTC 6 0.15 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 6 0.15 No Hit ATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGT 6 0.15 No Hit CTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGC 6 0.15 No Hit CTATTGGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGT 6 0.15 No Hit AACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGG 6 0.15 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 6 0.15 No Hit GAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGT 6 0.15 No Hit AAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATC 5 0.125 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 5 0.125 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 5 0.125 No Hit ATTTCTTAACCGCAGCAGTTTCTACCCCTGCCAATAGTTTAGCACACTCT 5 0.125 No Hit GAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAAT 5 0.125 No Hit GTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTT 5 0.125 No Hit AAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAG 5 0.125 No Hit ATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCAC 5 0.125 No Hit GATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTC 5 0.125 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 5 0.125 No Hit CTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATT 5 0.125 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 5 0.125 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 5 0.125 No Hit GCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAG 5 0.125 No Hit CAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTA 5 0.125 No Hit ATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTG 5 0.125 No Hit GATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCG 5 0.125 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 5 0.125 No Hit ATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.037500000000000006 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.0875 0.0 0.0 0.0 0.0 86-87 0.1125 0.0 0.0 0.0 0.0 88-89 0.1375 0.0 0.0 0.0 0.0 90-91 0.15 0.0 0.0 0.0 0.0 92-93 0.15 0.0 0.0 0.0 0.0 94-95 0.175 0.0 0.0 0.0 0.0 96-97 0.225 0.0 0.0 0.0 0.0 98-99 0.25 0.0 0.0 0.0 0.0 100-101 0.25 0.0 0.0 0.0 0.0 102-103 0.3125 0.0 0.0 0.0 0.0 104-105 0.325 0.0 0.0 0.0 0.0 106-107 0.36250000000000004 0.0 0.0 0.0 0.0 108-109 0.4875 0.0 0.0 0.0 0.0 110-111 0.6125 0.0 0.0 0.0 0.0 112-113 0.7125 0.0 0.0 0.0 0.0 114-115 0.775 0.0 0.0 0.0 0.0 116-117 0.825 0.0 0.0 0.0 0.0 118-119 0.8875 0.0 0.0 0.0 0.0 120-121 1.0 0.0 0.0 0.0 0.0 122-123 1.125 0.0 0.0 0.0 0.0 124-125 1.1875 0.0 0.0 0.0 0.0 126-127 1.2625 0.0 0.0 0.0 0.0 128-129 1.35 0.0 0.0 0.0 0.0 130-131 1.5875 0.0 0.0 0.0 0.0 132-133 1.6625 0.0 0.0 0.0 0.0 134-135 1.85 0.0 0.0 0.0 0.0 136-137 1.9875 0.0 0.0 0.0 0.0 138 2.1 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTAGG 20 3.687869E-4 108.0 5 TGTAGTA 20 3.687869E-4 108.0 3 GTAGTAG 20 3.687869E-4 108.0 4 AGTAGGA 25 8.956223E-4 86.399994 6 CCTGTAG 25 8.956223E-4 86.399994 1 AGGAATC 25 8.956223E-4 86.399994 9 GTAGGAA 25 8.956223E-4 86.399994 7 TAGGAAT 40 0.005777437 54.0 8 >>END_MODULE Read 2541027 spots for ERR11006601.sra Written 2541027 spots for ERR11006601.sra Read 2541027 spots for ERR11006601.sra Written 2541027 spots for ERR11006601.sra Read 2541027 spots for ERR11006601.sra Written 2541027 spots for ERR11006601.sra Read 2541027 spots for ERR11006601.sra Written 2541027 spots for ERR11006601.sra Read 2541027 spots for ERR11006601.sra Written 2541027 spots for ERR11006601.sra Read 2541027 spots for ERR11006601.sra Written 2541027 spots for ERR11006601.sra Read 2541027 spots for ERR11006601.sra Written 2541027 spots for ERR11006601.sra Read 2541027 spots for ERR11006601.sra Written 2541027 spots for ERR11006601.sra Read 2541027 spots for ERR11006601.sra Written 2541027 spots for ERR11006601.sra Read 2541027 spots for ERR11006601.sra Written 2541027 spots for ERR11006601.sra Read 2541027 spots for ERR11006601.sra Written 2541027 spots for ERR11006601.sra Read 2541027 spots for ERR11006601.sra Written 2541027 spots for ERR11006601.sra Read 2541027 spots for ERR11006601.sra Written 2541027 spots for ERR11006601.sra Read 2541027 spots for ERR11006601.sra Written 2541027 spots for ERR11006601.sra Read 2541027 spots for ERR11006601.sra Written 2541027 spots for ERR11006601.sra Read 2541027 spots for ERR11006601.sra Written 2541027 spots for ERR11006601.sra Read 2541027 spots for ERR11006601.sra Written 2541027 spots for ERR11006601.sra Read 2541027 spots for ERR11006601.sra Written 2541027 spots for ERR11006601.sra Read 2541041 spots for ERR11006601.sra Written 2541041 spots for ERR11006601.sra Read 2541027 spots for ERR11006601.sra Written 2541027 spots for ERR11006601.sra SRR ids: ['ERR11006601.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_wr62jwc1 ERR11006601.sra spots: 50820554 blocks: [[1, 2541027], [2541028, 5082054], [5082055, 7623081], [7623082, 10164108], [10164109, 12705135], [12705136, 15246162], [15246163, 17787189], [17787190, 20328216], [20328217, 22869243], [22869244, 25410270], [25410271, 27951297], [27951298, 30492324], [30492325, 33033351], [33033352, 35574378], [35574379, 38115405], [38115406, 40656432], [40656433, 43197459], [43197460, 45738486], [45738487, 48279513], [48279514, 50820554]] ERR11006601 file size 18673426 ERR11006601 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006601 ERR11006601_1.fastq ERR11006601_2.fastq Input file: ERR11006601_1.fastq Paired file: ERR11006601_2.fastq trimmed: ERR11006601-trimmed-pair1.fastq, ERR11006601-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 19:17:17 2024 >> started Fri Dec 6 19:18:29 2024 >> done (71.482s) 50820554 read pairs processed; of these: 422 ( 0.00%) short read pairs filtered out after trimming by size control 1190 ( 0.00%) empty read pairs filtered out after trimming by size control 50818942 (100.00%) read pairs available; of these: 1815597 ( 3.57%) trimmed read pairs available after processing 49003345 (96.43%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 20 0.00% 19 25 0.00% 20 26 0.00% 21 24 0.00% 22 40 0.00% 23 18 0.00% 24 38 0.00% 25 3308 0.01% 26 46 0.00% 27 19 0.00% 28 30 0.00% 29 45 0.00% 30 42 0.00% 31 85 0.00% 32 44 0.00% 33 28 0.00% 34 37 0.00% 35 35 0.00% 36 36 0.00% 37 49 0.00% 38 46 0.00% 39 49 0.00% 40 56 0.00% 41 46 0.00% 42 61 0.00% 43 77 0.00% 44 46 0.00% 45 74 0.00% 46 82 0.00% 47 70 0.00% 48 85 0.00% 49 113 0.00% 50 106 0.00% 51 126 0.00% 52 134 0.00% 53 168 0.00% 54 166 0.00% 55 339 0.00% 56 220 0.00% 57 221 0.00% 58 236 0.00% 59 244 0.00% 60 294 0.00% 61 337 0.00% 62 433 0.00% 63 439 0.00% 64 496 0.00% 65 509 0.00% 66 559 0.00% 67 579 0.00% 68 722 0.00% 69 675 0.00% 70 723 0.00% 71 786 0.00% 72 959 0.00% 73 1130 0.00% 74 1298 0.00% 75 1420 0.00% 76 1565 0.00% 77 1628 0.00% 78 1726 0.00% 79 1893 0.00% 80 1940 0.00% 81 2267 0.00% 82 2488 0.00% 83 2667 0.01% 84 2962 0.01% 85 3457 0.01% 86 3784 0.01% 87 4184 0.01% 88 4501 0.01% 89 4807 0.01% 90 5094 0.01% 91 5048 0.01% 92 5483 0.01% 93 5830 0.01% 94 6284 0.01% 95 6963 0.01% 96 7292 0.01% 97 7871 0.02% 98 8743 0.02% 99 9160 0.02% 100 9374 0.02% 101 9723 0.02% 102 10188 0.02% 103 10599 0.02% 104 11314 0.02% 105 12225 0.02% 106 13599 0.03% 107 13756 0.03% 108 14665 0.03% 109 15044 0.03% 110 16109 0.03% 111 16749 0.03% 112 17803 0.04% 113 17707 0.03% 114 18632 0.04% 115 19987 0.04% 116 22139 0.04% 117 22899 0.05% 118 23104 0.05% 119 25144 0.05% 120 26159 0.05% 121 27201 0.05% 122 28962 0.06% 123 28950 0.06% 124 30996 0.06% 125 32994 0.06% 126 31447 0.06% 127 31469 0.06% 128 33741 0.07% 129 35802 0.07% 130 39450 0.08% 131 41359 0.08% 132 41592 0.08% 133 40272 0.08% 134 42247 0.08% 135 42160 0.08% 136 43736 0.09% 137 45054 0.09% 138 47892 0.09% 139 50626 0.10% 140 51816 0.10% 141 54965 0.11% 142 57912 0.11% 143 58281 0.11% 144 58537 0.12% 145 62645 0.12% 146 68839 0.14% 147 68484 0.13% 148 71997 0.14% 149 73496 0.14% 150 49003345 96.43% 50818942 reads passed initial QC criterion=sequence-density sequence-density=0.17 sequence-density-rank=1 fanout-score=1.00 fanout-score-rank=25 prefix-density=0.01 prefix-fanout=1.0 sequence=CCCCCCCCCCGGGGGCCCAGGTATCATATACACCGCCAAAATAAAGAGCCTTGAGTACTAGAAGAAAAGCACCTAGACCTAACAAAATTAAATGAATACCCAAAATTGTAGTCATTTTATTTCTATCTTTCCATACATAACCAAAGAATGGAAAAGATTCCTCAAGAGTCTCGGGTCCCAGAAGCGCGTGATAAATGCCACCGAAGCCTAAGACTGCGGAGGAAATTAGGTGAAGTACTCCAGATACAAAGTACGGAAAAGTATCGAGAACTTCTCCCCCTGGCCCGACTCCCCAACCTAGGGTAGCTAAGTGTGGAAGTAAAATCAACCCTTGTTCATACATGGGCTTTTCTGGCACGAAATGGGCCACTTCAAATAGGTTCATTGCTCCGGCCCAGAATACGATTAATCCGGCATGGGCTACGTGGGCGCCAAGTAGTTTACCGGACAAATTGATAAGTCTAGCATTCCCAGCCCACCAAGCAAACCCGGTGGTTTCTTGGTCACGACC criterion=fanout-score sequence-density=0.16 sequence-density-rank=3 fanout-score=31.34 fanout-score-rank=1 prefix-density=4.98 prefix-fanout=1.0 sequence=CAAAATAACCAGGAGCAGCCACA criterion=sequence-density sequence-density=0.20 sequence-density-rank=1 fanout-score=1.00 fanout-score-rank=43 prefix-density=0.02 prefix-fanout=1.0 sequence=GGGGGGGGGGATGTAAGAAAAATAACCAACTTGACCCTTAGCCCCGGTGTTATATTTGGTTATTTACTAAAATCCCCTTTTGGGGGAGAAGGCTGGATTGTTAGTGTGGATGATTTAGAAGATATAATTGGTGGACATGTATGGTTGGGTTTCATTTGTGTATTTGGCGGAATTTGGCATATCTTAACCAAACCTTTCGCATGGGCTCGCCGTGCATTTGTATGGTCTGGAGAAGCTTACTTGTCTTATAGTTTAGCTGCTTTATCTGTCTTTGGTTTTATCGCTTGTTGTTTTGTCTGGTTCAATAATACGGCTTATCCGAGTGAGTTTTATGGACCCACCGGCCCAGAAGCTTCTCAAGCTCAAGCATTTACTTTTCTAGTTAGAGACCAGCGTCTTGGAGCTAATGTGGGATCTGCTCAAGGACCCACAGGTTTAGGTAAATATCTAATGCGTTCCCCAACGGGAGAGGTTATCTTTGGAGGGGAAACTATGCGTTTTTGGGACCTCC criterion=fanout-score sequence-density=0.07 sequence-density-rank=24 fanout-score=94.77 fanout-score-rank=1 prefix-density=6.60 prefix-fanout=1.0 sequence=CTGGATAACTATCACTGAAAATC ERR11006601 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 19:19:14 Started mapping on | Dec 06 19:19:15 Finished on | Dec 06 19:25:09 Mapping speed, Million of reads per hour | 516.80 Number of input reads | 50818942 Average input read length | 298 UNIQUE READS: Uniquely mapped reads number | 39312862 Uniquely mapped reads % | 77.36% Average mapped length | 297.38 Number of splices: Total | 16597474 Number of splices: Annotated (sjdb) | 15611598 Number of splices: GT/AG | 16311233 Number of splices: GC/AG | 184824 Number of splices: AT/AC | 13923 Number of splices: Non-canonical | 87494 Mismatch rate per base, % | 0.32% Deletion rate per base | 0.02% Deletion average length | 1.76 Insertion rate per base | 0.01% Insertion average length | 2.07 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 9747883 % of reads mapped to multiple loci | 19.18% Number of reads mapped to too many loci | 8737 % of reads mapped to too many loci | 0.02% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.87% % of reads unmapped: other | 0.57% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1758197 1758197 1758197 N_multimapping 9747883 9747883 9747883 N_noFeature 10994055 36767553 12766390 N_ambiguous 1144597 39008 352442 UnstrandedReadsAssigned:27174210 PositiveStrandReadsAssigned:2506301 NegativeStrandReadsAssigned:26194030 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006601 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006601-trimmed-pair1.fastq ERR11006601-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 50,818,942 reads, 31,457,472 reads pseudoaligned [quant] estimated average fragment length: 237.622 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,162 rounds 52973 ERR11006601.ke.tsv 35125 ERR11006601.se.tsv 88098 total ==> ERR11006601.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 699.614 0 0 PNS24247 1044 807.378 64.4728 2.86835 PNS24249 1928 1691.38 142.747 3.03152 PNS24246 1044 807.378 64.4728 2.86835 PNS24248 1044 807.378 64.4728 2.86835 PNS24244 1471 1234.38 77.8345 2.26494 PNS24243 293 70.856 1 0.50694 KQK14069 1603 1366.38 990.289 26.033 KQK14071 474 238.868 43.8504 6.59399 ==> ERR11006601.se.tsv <== BRADI_1g14170v3 1100 BRADI_1g53295v3 1070 BRADI_1g59795v3 342 BRADI_1g07683v3 0 BRADI_1g00485v3 18 BRADI_1g20270v3 490 BRADI_1g74790v3 522 BRADI_1g09890v3 1 BRADI_1g77505v3 141 BRADI_1g48960v3 0 ERR11006601 completed mapping pipeline successfully