Starting /dee2/code/volunteer_pipeline.sh ERR11006602 current disk space = 1549882339328 free memory = 1597987180 ERR11006602 SRAfilesize 29dca349961b51ca4bc73e855ec9c7bb ERR11006602.sra ERR11006602.sra file validated ERR11006602 is paired end ERR11006602 is conventional basespace ERR11006602 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006602_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.448 37.0 37.0 37.0 37.0 37.0 2 36.5065 37.0 37.0 37.0 37.0 37.0 3 36.4955 37.0 37.0 37.0 37.0 37.0 4 36.62 37.0 37.0 37.0 37.0 37.0 5 36.605 37.0 37.0 37.0 37.0 37.0 6 36.6005 37.0 37.0 37.0 37.0 37.0 7 36.665 37.0 37.0 37.0 37.0 37.0 8 36.58 37.0 37.0 37.0 37.0 37.0 9 36.7115 37.0 37.0 37.0 37.0 37.0 10-14 36.6182 37.0 37.0 37.0 37.0 37.0 15-19 36.589000000000006 37.0 37.0 37.0 37.0 37.0 20-24 36.5455 37.0 37.0 37.0 37.0 37.0 25-29 36.5084 37.0 37.0 37.0 37.0 37.0 30-34 36.4957 37.0 37.0 37.0 37.0 37.0 35-39 36.5015 37.0 37.0 37.0 37.0 37.0 40-44 36.4698 37.0 37.0 37.0 37.0 37.0 45-49 36.4697 37.0 37.0 37.0 37.0 37.0 50-54 36.4561 37.0 37.0 37.0 37.0 37.0 55-59 36.431 37.0 37.0 37.0 37.0 37.0 60-64 36.357899999999994 37.0 37.0 37.0 37.0 37.0 65-69 36.4181 37.0 37.0 37.0 37.0 37.0 70-74 36.37140000000001 37.0 37.0 37.0 37.0 37.0 75-79 36.2903 37.0 37.0 37.0 37.0 37.0 80-84 36.3214 37.0 37.0 37.0 37.0 37.0 85-89 36.324 37.0 37.0 37.0 37.0 37.0 90-94 36.2245 37.0 37.0 37.0 37.0 37.0 95-99 36.3195 37.0 37.0 37.0 37.0 37.0 100-104 36.2061 37.0 37.0 37.0 37.0 37.0 105-109 36.2282 37.0 37.0 37.0 37.0 37.0 110-114 36.1417 37.0 37.0 37.0 37.0 37.0 115-119 36.189099999999996 37.0 37.0 37.0 37.0 37.0 120-124 36.1057 37.0 37.0 37.0 37.0 37.0 125-129 36.1246 37.0 37.0 37.0 37.0 37.0 130-134 36.0535 37.0 37.0 37.0 37.0 37.0 135-139 35.9771 37.0 37.0 37.0 37.0 37.0 140-144 35.997099999999996 37.0 37.0 37.0 37.0 37.0 145-149 35.9077 37.0 37.0 37.0 37.0 37.0 150 35.8225 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 0.0 21 0.0 22 0.0 23 3.0 24 1.0 25 2.0 26 7.0 27 8.0 28 10.0 29 18.0 30 30.0 31 31.0 32 42.0 33 62.0 34 103.0 35 224.0 36 3021.0 37 437.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.85 11.875 15.425 35.85 2 28.349999999999998 9.575 28.1 33.975 3 21.05 11.75 27.35 39.85 4 28.65 14.924999999999999 24.55 31.874999999999996 5 27.375 18.55 27.150000000000002 26.924999999999997 6 26.650000000000002 27.35 21.925 24.075 7 20.025000000000002 26.8 32.0 21.175 8 16.875 28.525 33.550000000000004 21.05 9 17.95 25.525 36.275 20.25 10-14 20.985 31.055 26.91 21.05 15-19 20.885 32.555 25.335 21.224999999999998 20-24 19.25 29.025000000000002 28.060000000000002 23.665 25-29 21.584999999999997 30.855 26.605 20.955 30-34 23.330000000000002 30.595 25.069999999999997 21.005 35-39 22.884999999999998 30.830000000000002 25.35 20.935000000000002 40-44 20.669999999999998 29.904999999999998 25.695 23.73 45-49 21.235 29.26 26.645000000000003 22.86 50-54 21.41 31.115 25.835 21.64 55-59 22.175 29.5 24.154999999999998 24.169999999999998 60-64 20.200000000000003 31.069999999999997 25.330000000000002 23.400000000000002 65-69 21.455 29.205 25.205 24.135 70-74 22.89 29.195 23.11 24.805 75-79 22.495 29.294999999999998 25.22 22.99 80-84 22.03 29.065 24.560000000000002 24.345 85-89 23.925 29.085 25.05 21.94 90-94 22.235 28.265 26.96 22.54 95-99 24.185000000000002 28.849999999999998 23.625 23.34 100-104 21.525 28.854999999999997 25.064999999999998 24.555 105-109 22.195 28.244999999999997 26.44 23.119999999999997 110-114 24.46 26.845000000000002 25.019999999999996 23.674999999999997 115-119 20.59 30.03 26.02 23.36 120-124 20.76 29.49 24.965 24.785 125-129 21.295 30.115 23.005 25.585 130-134 23.255 29.78 24.26 22.705000000000002 135-139 23.28 29.535 23.474999999999998 23.71 140-144 24.85 27.325 26.415 21.41 145-149 22.845 29.494999999999997 24.995 22.665 150 22.15 26.3 27.800000000000004 23.75 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 0.0 20 1.0 21 2.5 22 2.0 23 3.0 24 4.0 25 2.5 26 3.5 27 7.5 28 9.5 29 7.5 30 11.5 31 19.5 32 28.5 33 33.0 34 36.0 35 41.5 36 88.0 37 162.5 38 249.0 39 269.0 40 241.0 41 270.5 42 287.5 43 258.5 44 211.0 45 202.5 46 202.0 47 158.5 48 122.0 49 108.5 50 92.0 51 78.5 52 63.0 53 53.0 54 42.5 55 35.5 56 30.5 57 22.0 58 25.5 59 27.5 60 29.0 61 30.5 62 26.0 63 24.0 64 48.0 65 68.5 66 45.0 67 24.5 68 29.0 69 24.5 70 18.5 71 16.5 72 14.5 73 14.5 74 12.5 75 12.5 76 11.0 77 9.5 78 8.5 79 4.5 80 3.0 81 2.0 82 1.5 83 1.5 84 0.0 85 0.5 86 0.5 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.425 #Duplication Level Percentage of deduplicated Percentage of total 1 77.88125727590221 50.175000000000004 2 14.939852541715174 19.25 3 3.104384943733023 6.0 4 1.474582848273186 3.8 5 0.5044625533566163 1.625 6 0.38804811796662786 1.5 7 0.46565774155995343 2.1 8 0.07760962359332557 0.4 9 0.23282887077997672 1.35 >10 0.892510671323244 11.425 >50 0.038804811796662786 2.375 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 95 2.375 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 44 1.0999999999999999 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 43 1.075 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 38 0.95 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 26 0.65 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 25 0.625 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 24 0.6 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 23 0.575 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 22 0.5499999999999999 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 20 0.5 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 20 0.5 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 19 0.475 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 19 0.475 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 18 0.44999999999999996 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 15 0.375 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 14 0.35000000000000003 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 13 0.325 No Hit TCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGT 13 0.325 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 11 0.27499999999999997 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 10 0.25 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 10 0.25 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 10 0.25 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 10 0.25 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 10 0.25 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 9 0.22499999999999998 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 9 0.22499999999999998 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 9 0.22499999999999998 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 9 0.22499999999999998 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 9 0.22499999999999998 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 9 0.22499999999999998 No Hit GGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAG 8 0.2 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 8 0.2 No Hit ACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 7 0.17500000000000002 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 7 0.17500000000000002 No Hit GATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAG 7 0.17500000000000002 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 7 0.17500000000000002 No Hit AGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATAT 7 0.17500000000000002 No Hit TGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTG 7 0.17500000000000002 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 7 0.17500000000000002 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 7 0.17500000000000002 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 7 0.17500000000000002 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 7 0.17500000000000002 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 7 0.17500000000000002 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 7 0.17500000000000002 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 6 0.15 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 6 0.15 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 6 0.15 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 6 0.15 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 6 0.15 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 6 0.15 No Hit TGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCT 6 0.15 No Hit ATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATA 6 0.15 No Hit CACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCG 6 0.15 No Hit CACCCGTATGACTATCGAAATCGTGAGCATCAGCATGTAGGTTCCAGATC 6 0.15 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 5 0.125 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 5 0.125 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 5 0.125 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 5 0.125 No Hit TCCCACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAAC 5 0.125 No Hit TCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGT 5 0.125 No Hit CCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAA 5 0.125 No Hit ATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAG 5 0.125 No Hit AGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTAT 5 0.125 No Hit TCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAA 5 0.125 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 5 0.125 No Hit TAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGA 5 0.125 No Hit AGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.025 0.0 0.0 0.0 0.0 118-119 0.05 0.0 0.0 0.0 0.0 120-121 0.0875 0.0 0.0 0.0 0.0 122-123 0.125 0.0 0.0 0.0 0.0 124-125 0.125 0.0 0.0 0.0 0.0 126-127 0.125 0.0 0.0 0.0 0.0 128-129 0.125 0.0 0.0 0.0 0.0 130-131 0.125 0.0 0.0 0.0 0.0 132-133 0.1375 0.0 0.0 0.0 0.0 134-135 0.15 0.0 0.0 0.0 0.0 136-137 0.175 0.0 0.0 0.0 0.0 138 0.175 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCAGAC 10 0.006973645 144.0 1 GACATAC 10 0.006973645 144.0 5 TACGCAA 10 0.006973645 144.0 9 ACATACG 10 0.006973645 144.0 6 ATACGCA 10 0.006973645 144.0 8 CAGACAT 10 0.006973645 144.0 3 AGACATA 10 0.006973645 144.0 4 CATACGC 10 0.006973645 144.0 7 >>END_MODULE ERR11006602 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006602_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.12 37.0 37.0 37.0 37.0 37.0 2 36.286 37.0 37.0 37.0 37.0 37.0 3 36.3695 37.0 37.0 37.0 37.0 37.0 4 36.3725 37.0 37.0 37.0 37.0 37.0 5 36.274 37.0 37.0 37.0 37.0 37.0 6 36.3045 37.0 37.0 37.0 37.0 37.0 7 36.411 37.0 37.0 37.0 37.0 37.0 8 36.345 37.0 37.0 37.0 37.0 37.0 9 36.372 37.0 37.0 37.0 37.0 37.0 10-14 36.3549 37.0 37.0 37.0 37.0 37.0 15-19 36.356700000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.3397 37.0 37.0 37.0 37.0 37.0 25-29 36.292 37.0 37.0 37.0 37.0 37.0 30-34 36.2755 37.0 37.0 37.0 37.0 37.0 35-39 36.2162 37.0 37.0 37.0 37.0 37.0 40-44 36.238200000000006 37.0 37.0 37.0 37.0 37.0 45-49 36.2223 37.0 37.0 37.0 37.0 37.0 50-54 36.1928 37.0 37.0 37.0 37.0 37.0 55-59 36.160399999999996 37.0 37.0 37.0 37.0 37.0 60-64 36.0907 37.0 37.0 37.0 37.0 37.0 65-69 36.0855 37.0 37.0 37.0 37.0 37.0 70-74 36.0283 37.0 37.0 37.0 37.0 37.0 75-79 36.0758 37.0 37.0 37.0 37.0 37.0 80-84 36.03810000000001 37.0 37.0 37.0 37.0 37.0 85-89 36.0205 37.0 37.0 37.0 37.0 37.0 90-94 35.97 37.0 37.0 37.0 37.0 37.0 95-99 35.945499999999996 37.0 37.0 37.0 37.0 37.0 100-104 35.9617 37.0 37.0 37.0 37.0 37.0 105-109 35.872 37.0 37.0 37.0 37.0 37.0 110-114 35.8713 37.0 37.0 37.0 37.0 37.0 115-119 35.73909999999999 37.0 37.0 37.0 37.0 37.0 120-124 35.862100000000005 37.0 37.0 37.0 37.0 37.0 125-129 35.717 37.0 37.0 37.0 37.0 37.0 130-134 35.6956 37.0 37.0 37.0 37.0 37.0 135-139 35.808 37.0 37.0 37.0 37.0 37.0 140-144 35.6378 37.0 37.0 37.0 37.0 37.0 145-149 35.612 37.0 37.0 37.0 37.0 37.0 150 35.6045 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 0.0 17 0.0 18 2.0 19 1.0 20 2.0 21 2.0 22 7.0 23 1.0 24 6.0 25 4.0 26 8.0 27 9.0 28 15.0 29 23.0 30 28.0 31 30.0 32 35.0 33 59.0 34 135.0 35 493.0 36 2971.0 37 168.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.095477386934675 24.49748743718593 17.08542713567839 29.321608040201003 2 27.075 24.15 32.35 16.425 3 21.45 27.800000000000004 32.375 18.375 4 24.325 27.6 27.450000000000003 20.625 5 23.625 31.2 26.3 18.875 6 21.625 34.699999999999996 25.4 18.275 7 19.875 22.2 39.675 18.25 8 22.275 23.799999999999997 29.95 23.974999999999998 9 23.825 21.975 33.7 20.5 10-14 24.195 26.115 28.244999999999997 21.445 15-19 24.349999999999998 25.34 29.509999999999998 20.8 20-24 24.495 25.540000000000003 28.505000000000003 21.46 25-29 24.725 24.45 29.459999999999997 21.365000000000002 30-34 24.485 25.1 28.74 21.675 35-39 23.555 25.240000000000002 29.145 22.06 40-44 23.57 25.795 29.53 21.105 45-49 23.07 26.71 28.38 21.84 50-54 24.060000000000002 25.8 28.08 22.06 55-59 23.78 25.515 27.73 22.975 60-64 23.375 24.59 29.075 22.96 65-69 24.445 24.92 28.645 21.990000000000002 70-74 24.88 25.080000000000002 29.315 20.724999999999998 75-79 24.615000000000002 24.715 29.25 21.42 80-84 25.485000000000003 25.27 27.595 21.65 85-89 24.255 25.95 27.22 22.575 90-94 24.34 25.555 27.725 22.38 95-99 24.735 24.755 28.555000000000003 21.955 100-104 24.425 23.919999999999998 29.37 22.285 105-109 25.985000000000003 23.24 29.21 21.565 110-114 24.67 23.93 29.005 22.395 115-119 23.645 25.83 28.294999999999998 22.23 120-124 24.72 25.869999999999997 27.505000000000003 21.905 125-129 23.44 26.365 28.349999999999998 21.845 130-134 22.78 26.395000000000003 28.249999999999996 22.575 135-139 23.56 25.72 29.265 21.455 140-144 23.34 25.19 29.69 21.78 145-149 23.580000000000002 24.295 30.044999999999998 22.08 150 22.650000000000002 23.674999999999997 29.325000000000003 24.349999999999998 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 1.0 20 1.0 21 0.0 22 1.5 23 2.5 24 4.0 25 9.5 26 10.0 27 10.0 28 14.5 29 15.5 30 20.0 31 30.5 32 34.0 33 35.5 34 53.5 35 72.0 36 102.0 37 177.0 38 223.5 39 220.5 40 225.0 41 245.0 42 222.5 43 220.0 44 233.0 45 203.5 46 187.5 47 156.0 48 129.5 49 103.5 50 80.0 51 75.5 52 59.0 53 46.0 54 38.5 55 25.5 56 26.5 57 31.5 58 33.0 59 33.0 60 32.5 61 34.5 62 32.5 63 48.5 64 60.5 65 49.0 66 38.0 67 34.5 68 34.5 69 29.5 70 28.5 71 30.5 72 25.5 73 18.0 74 16.5 75 15.5 76 11.5 77 8.5 78 7.5 79 8.0 80 6.0 81 2.5 82 2.0 83 4.0 84 4.0 85 2.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.025 #Duplication Level Percentage of deduplicated Percentage of total 1 78.31679529584711 53.27499999999999 2 14.112458654906284 19.2 3 3.601617052554208 7.35 4 1.396545387725101 3.8 5 0.9555310547592797 3.25 6 0.2940095553105476 1.2 7 0.2205071664829107 1.05 8 0.1470047776552738 0.8 9 0.2205071664829107 1.35 >10 0.6982726938625505 7.324999999999999 >50 0.03675119441381845 1.4000000000000001 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 56 1.4000000000000001 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 26 0.65 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 24 0.6 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 22 0.5499999999999999 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 19 0.475 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 18 0.44999999999999996 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 17 0.42500000000000004 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 17 0.42500000000000004 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 17 0.42500000000000004 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 15 0.375 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 14 0.35000000000000003 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 13 0.325 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 13 0.325 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 13 0.325 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 12 0.3 No Hit TGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACT 12 0.3 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 11 0.27499999999999997 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 10 0.25 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 10 0.25 No Hit TCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTG 10 0.25 No Hit TGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGA 9 0.22499999999999998 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 9 0.22499999999999998 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 9 0.22499999999999998 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 9 0.22499999999999998 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 9 0.22499999999999998 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 9 0.22499999999999998 No Hit CTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAG 8 0.2 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 8 0.2 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 8 0.2 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 8 0.2 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 7 0.17500000000000002 No Hit CTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAG 7 0.17500000000000002 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 7 0.17500000000000002 No Hit GAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCG 7 0.17500000000000002 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 7 0.17500000000000002 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 7 0.17500000000000002 No Hit GTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCT 6 0.15 No Hit TATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACC 6 0.15 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 6 0.15 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 6 0.15 No Hit GTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGA 6 0.15 No Hit GTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACA 6 0.15 No Hit CATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGGCG 6 0.15 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 6 0.15 No Hit ATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGC 5 0.125 No Hit GTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTC 5 0.125 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 5 0.125 No Hit TATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACA 5 0.125 No Hit GACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCC 5 0.125 No Hit GGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCG 5 0.125 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 5 0.125 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 5 0.125 No Hit GTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCAT 5 0.125 No Hit AATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTA 5 0.125 No Hit TACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGT 5 0.125 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 5 0.125 No Hit GCAGATTTTTTAGTCCATCACATTCACGCATTTACCATCCATGTGACTGT 5 0.125 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 5 0.125 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 5 0.125 No Hit ATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTAC 5 0.125 No Hit TGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATT 5 0.125 No Hit TAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGA 5 0.125 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 5 0.125 No Hit GTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGC 5 0.125 No Hit AATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTA 5 0.125 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 5 0.125 No Hit GAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTT 5 0.125 No Hit ATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTG 5 0.125 No Hit TGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACT 5 0.125 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.025 0.0 0.0 0.0 0.0 118-119 0.05 0.0 0.0 0.0 0.0 120-121 0.0875 0.0 0.0 0.0 0.0 122-123 0.125 0.0 0.0 0.0 0.0 124-125 0.125 0.0 0.0 0.0 0.0 126-127 0.125 0.0 0.0 0.0 0.0 128-129 0.125 0.0 0.0 0.0 0.0 130-131 0.125 0.0 0.0 0.0 0.0 132-133 0.1375 0.0 0.0 0.0 0.0 134-135 0.15 0.0 0.0 0.0 0.0 136-137 0.175 0.0 0.0 0.0 0.0 138 0.175 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGTCG 10 0.006973645 144.0 5 ACGTCGA 10 0.006973645 144.0 6 CGCCAAC 10 0.006973645 144.0 1 >>END_MODULE Read 2638601 spots for ERR11006602.sra Written 2638601 spots for ERR11006602.sra Read 2638601 spots for ERR11006602.sra Written 2638601 spots for ERR11006602.sra Read 2638601 spots for ERR11006602.sra Written 2638601 spots for ERR11006602.sra Read 2638601 spots for ERR11006602.sra Written 2638601 spots for ERR11006602.sra Read 2638601 spots for ERR11006602.sra Written 2638601 spots for ERR11006602.sra Read 2638601 spots for ERR11006602.sra Written 2638601 spots for ERR11006602.sra Read 2638601 spots for ERR11006602.sra Written 2638601 spots for ERR11006602.sra Read 2638601 spots for ERR11006602.sra Written 2638601 spots for ERR11006602.sra Read 2638601 spots for ERR11006602.sra Written 2638601 spots for ERR11006602.sra Read 2638601 spots for ERR11006602.sra Written 2638601 spots for ERR11006602.sra Read 2638601 spots for ERR11006602.sra Written 2638601 spots for ERR11006602.sra Read 2638601 spots for ERR11006602.sra Written 2638601 spots for ERR11006602.sra Read 2638601 spots for ERR11006602.sra Written 2638601 spots for ERR11006602.sra Read 2638601 spots for ERR11006602.sra Written 2638601 spots for ERR11006602.sra Read 2638601 spots for ERR11006602.sra Written 2638601 spots for ERR11006602.sra Read 2638618 spots for ERR11006602.sra Written 2638618 spots for ERR11006602.sra Read 2638601 spots for ERR11006602.sra Written 2638601 spots for ERR11006602.sra Read 2638601 spots for ERR11006602.sra Written 2638601 spots for ERR11006602.sra Read 2638601 spots for ERR11006602.sra Written 2638601 spots for ERR11006602.sra Read 2638601 spots for ERR11006602.sra Written 2638601 spots for ERR11006602.sra SRR ids: ['ERR11006602.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_gwafbs0r ERR11006602.sra spots: 52772037 blocks: [[1, 2638601], [2638602, 5277202], [5277203, 7915803], [7915804, 10554404], [10554405, 13193005], [13193006, 15831606], [15831607, 18470207], [18470208, 21108808], [21108809, 23747409], [23747410, 26386010], [26386011, 29024611], [29024612, 31663212], [31663213, 34301813], [34301814, 36940414], [36940415, 39579015], [39579016, 42217616], [42217617, 44856217], [44856218, 47494818], [47494819, 50133419], [50133420, 52772037]] ERR11006602 file size 19391035 ERR11006602 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006602 ERR11006602_1.fastq ERR11006602_2.fastq Input file: ERR11006602_1.fastq Paired file: ERR11006602_2.fastq trimmed: ERR11006602-trimmed-pair1.fastq, ERR11006602-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 19:18:46 2024 >> started Fri Dec 6 19:19:45 2024 >> done (59.094s) 52772037 read pairs processed; of these: 540 ( 0.00%) short read pairs filtered out after trimming by size control 3857 ( 0.01%) empty read pairs filtered out after trimming by size control 52767640 (99.99%) read pairs available; of these: 267698 ( 0.51%) trimmed read pairs available after processing 52499942 (99.49%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 22 0.00% 19 30 0.00% 20 23 0.00% 21 33 0.00% 22 69 0.00% 23 29 0.00% 24 26 0.00% 25 24 0.00% 26 25 0.00% 27 27 0.00% 28 31 0.00% 29 29 0.00% 30 36 0.00% 31 36 0.00% 32 26 0.00% 33 39 0.00% 34 24 0.00% 35 29 0.00% 36 39 0.00% 37 40 0.00% 38 21 0.00% 39 46 0.00% 40 29 0.00% 41 39 0.00% 42 38 0.00% 43 45 0.00% 44 36 0.00% 45 45 0.00% 46 54 0.00% 47 64 0.00% 48 52 0.00% 49 65 0.00% 50 53 0.00% 51 49 0.00% 52 36 0.00% 53 68 0.00% 54 61 0.00% 55 63 0.00% 56 75 0.00% 57 73 0.00% 58 101 0.00% 59 85 0.00% 60 98 0.00% 61 110 0.00% 62 103 0.00% 63 126 0.00% 64 131 0.00% 65 167 0.00% 66 146 0.00% 67 129 0.00% 68 146 0.00% 69 171 0.00% 70 166 0.00% 71 166 0.00% 72 182 0.00% 73 195 0.00% 74 244 0.00% 75 259 0.00% 76 287 0.00% 77 286 0.00% 78 307 0.00% 79 284 0.00% 80 343 0.00% 81 342 0.00% 82 383 0.00% 83 375 0.00% 84 419 0.00% 85 462 0.00% 86 556 0.00% 87 529 0.00% 88 608 0.00% 89 622 0.00% 90 665 0.00% 91 771 0.00% 92 783 0.00% 93 758 0.00% 94 762 0.00% 95 847 0.00% 96 921 0.00% 97 942 0.00% 98 998 0.00% 99 1104 0.00% 100 1190 0.00% 101 1288 0.00% 102 1358 0.00% 103 1399 0.00% 104 1435 0.00% 105 1492 0.00% 106 1558 0.00% 107 1725 0.00% 108 1719 0.00% 109 1890 0.00% 110 2158 0.00% 111 2057 0.00% 112 2315 0.00% 113 2372 0.00% 114 2550 0.00% 115 2579 0.00% 116 2861 0.01% 117 3015 0.01% 118 3075 0.01% 119 3340 0.01% 120 3547 0.01% 121 3704 0.01% 122 4066 0.01% 123 4166 0.01% 124 4392 0.01% 125 4880 0.01% 126 4454 0.01% 127 4430 0.01% 128 4608 0.01% 129 5074 0.01% 130 5407 0.01% 131 5896 0.01% 132 6180 0.01% 133 6037 0.01% 134 6201 0.01% 135 6184 0.01% 136 6432 0.01% 137 6643 0.01% 138 7123 0.01% 139 7704 0.01% 140 7543 0.01% 141 8319 0.02% 142 8796 0.02% 143 9503 0.02% 144 9785 0.02% 145 9822 0.02% 146 11673 0.02% 147 11132 0.02% 148 11382 0.02% 149 12511 0.02% 150 52499942 99.49% 52767640 reads passed initial QC criterion=sequence-density sequence-density=0.26 sequence-density-rank=1 fanout-score=1.94 fanout-score-rank=29 prefix-density=0.24 prefix-fanout=1.9 sequence=TCTAATTCAAAA criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=74.07 fanout-score-rank=1 prefix-density=0.26 prefix-fanout=1.9 sequence=AAAAACAGTAGAAGTAGAACAGGTATAAATAAGAAAATCTTAGTTAAGAGGGTTCATGTAAAGAACAGGTTCTAAATCACGATCGATTCCCTTTTCAAAACCTGCTGCAGCAGCTCGGGCTCTTCCTGCATGCCACAAATGGCCCACAAAAAAGAAGAATCCTAGAACAAAATGAGAAGTCGATAACCAACTTCTAGGAGAGACATAATTAACTGCATTGATCTCGGTAGCTACGCCACCCACGGAATTTAAAGAGCCTAAAGGAGCATGGGTCATATATTCCGCTGAACGTCGTTCTTGCCAAGGTTGTATGTCTTTTTTCAACCTACTCAAGTCCAAACCGTTGGGCCCCCTTAGAGGTTCTAACCATGGAGCACGGAGGTCCCAAAAACGCATAGTTTCCCCTCCAAAGATAACCTCTCCCGTTGGGGAACGCATTAGATATTTACCTAAACCTGTGGGTCCTTGAGCAGATCCCACATTAGCTCCAAGACGCTGGTCTCTAACTAGA criterion=sequence-density sequence-density=0.40 sequence-density-rank=1 fanout-score=2.02 fanout-score-rank=30 prefix-density=0.41 prefix-fanout=2.0 sequence=GCGGCTCTCCTA criterion=fanout-score sequence-density=0.01 sequence-density-rank=27 fanout-score=63.22 fanout-score-rank=1 prefix-density=0.10 prefix-fanout=9.4 sequence=TTTTTTTTTATGAGATTTTTGCTAAAGTTTCATTTACGCCTAATTCACATCGAGTAGACCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCACCACAAACAGAAACTAAAGCAAGTGTTGGATTTAAAGCTGGTGTTAAAGATTATAGATTGACTTACTACACCCCGGAGTATGAAACCAAGGATACTGATATCTTGGCAGCATTCCGAGTATCTCCTCAACCTGGGGTTCCGCCCGAAGAAGCAGGGGCTGCAGTAGCTGCCGAATCTTCTACTGGTACATGGACAACTGTTTGGACTGATGGACTTACTAGTCTTGATCGTTACAAAGGACGATGCTATCACATCGAGCCTGTTCCTGGGGAAGACAGTCAATGGATCTGTTATGTAGCTTATCCATTAGATCTATTTGAAGAGGGTTCCGTTACTAACATGTTTACTTCCATTGTAGGTAACGTATTTGGTTTCAAAGCCCTACGTGCTCTACGTCTG ERR11006602 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 19:20:22 Started mapping on | Dec 06 19:20:22 Finished on | Dec 06 19:27:35 Mapping speed, Million of reads per hour | 438.71 Number of input reads | 52767640 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 40622107 Uniquely mapped reads % | 76.98% Average mapped length | 298.47 Number of splices: Total | 17012856 Number of splices: Annotated (sjdb) | 15905307 Number of splices: GT/AG | 16600873 Number of splices: GC/AG | 216436 Number of splices: AT/AC | 17868 Number of splices: Non-canonical | 177679 Mismatch rate per base, % | 0.32% Deletion rate per base | 0.01% Deletion average length | 1.88 Insertion rate per base | 0.01% Insertion average length | 1.96 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 10071232 % of reads mapped to multiple loci | 19.09% Number of reads mapped to too many loci | 10182 % of reads mapped to too many loci | 0.02% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.67% % of reads unmapped: other | 0.24% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2074301 2074301 2074301 N_multimapping 10071232 10071232 10071232 N_noFeature 8713835 36919041 11336240 N_ambiguous 1855624 56416 788425 UnstrandedReadsAssigned:30052648 PositiveStrandReadsAssigned:3646650 NegativeStrandReadsAssigned:28497442 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006602 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006602-trimmed-pair1.fastq ERR11006602-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 52,767,640 reads, 31,441,384 reads pseudoaligned [quant] estimated average fragment length: 302.282 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,166 rounds 52973 ERR11006602.ke.tsv 35125 ERR11006602.se.tsv 88098 total ==> ERR11006602.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 635.128 0 0 PNS24247 1044 742.718 17.4618 0.676093 PNS24249 1928 1626.72 159.132 2.8131 PNS24246 1044 742.718 17.4618 0.676093 PNS24248 1044 742.718 17.4618 0.676093 PNS24244 1471 1169.72 82.4829 2.02779 PNS24243 293 44.9349 0 0 KQK14069 1603 1301.72 2504.38 55.3255 KQK14071 474 176.736 50.3098 8.18595 ==> ERR11006602.se.tsv <== BRADI_1g14170v3 2849 BRADI_1g53295v3 163 BRADI_1g59795v3 264 BRADI_1g07683v3 0 BRADI_1g00485v3 3 BRADI_1g20270v3 183 BRADI_1g74790v3 382 BRADI_1g09890v3 2 BRADI_1g77505v3 275 BRADI_1g48960v3 1 ERR11006602 completed mapping pipeline successfully