Starting /dee2/code/volunteer_pipeline.sh ERR11006603 current disk space = 1549751328768 free memory = 1377980544 ERR11006603 SRAfilesize 8a563a348291345220fcfd41bb9a12c8 ERR11006603.sra ERR11006603.sra file validated ERR11006603 is paired end ERR11006603 is conventional basespace ERR11006603 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006603_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.3255 37.0 37.0 37.0 37.0 37.0 2 36.42875 37.0 37.0 37.0 37.0 37.0 3 36.509 37.0 37.0 37.0 37.0 37.0 4 36.487 37.0 37.0 37.0 37.0 37.0 5 36.3405 37.0 37.0 37.0 37.0 37.0 6 36.4785 37.0 37.0 37.0 37.0 37.0 7 36.492 37.0 37.0 37.0 37.0 37.0 8 36.3835 37.0 37.0 37.0 37.0 37.0 9 36.425 37.0 37.0 37.0 37.0 37.0 10-14 36.5201 37.0 37.0 37.0 37.0 37.0 15-19 36.5034 37.0 37.0 37.0 37.0 37.0 20-24 36.4653 37.0 37.0 37.0 37.0 37.0 25-29 36.437400000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.32189999999999 37.0 37.0 37.0 37.0 37.0 35-39 36.3425 37.0 37.0 37.0 37.0 37.0 40-44 36.329899999999995 37.0 37.0 37.0 37.0 37.0 45-49 36.278 37.0 37.0 37.0 37.0 37.0 50-54 36.2786 37.0 37.0 37.0 37.0 37.0 55-59 36.2175 37.0 37.0 37.0 37.0 37.0 60-64 35.9788 37.0 37.0 37.0 37.0 37.0 65-69 36.1279 37.0 37.0 37.0 37.0 37.0 70-74 36.10039999999999 37.0 37.0 37.0 37.0 37.0 75-79 36.0173 37.0 37.0 37.0 37.0 37.0 80-84 35.999 37.0 37.0 37.0 37.0 37.0 85-89 36.0021 37.0 37.0 37.0 37.0 37.0 90-94 35.7556 37.0 37.0 37.0 37.0 37.0 95-99 35.83239999999999 37.0 37.0 37.0 37.0 37.0 100-104 35.874300000000005 37.0 37.0 37.0 37.0 37.0 105-109 35.7399 37.0 37.0 37.0 37.0 37.0 110-114 35.763099999999994 37.0 37.0 37.0 37.0 37.0 115-119 35.738600000000005 37.0 37.0 37.0 37.0 37.0 120-124 35.6228 37.0 37.0 37.0 37.0 37.0 125-129 35.723600000000005 37.0 37.0 37.0 37.0 37.0 130-134 35.6337 37.0 37.0 37.0 37.0 37.0 135-139 35.594899999999996 37.0 37.0 37.0 37.0 37.0 140-144 35.565999999999995 37.0 37.0 37.0 37.0 37.0 145-149 35.2981 37.0 37.0 37.0 29.8 37.0 150 35.4565 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 0.0 21 1.0 22 0.0 23 1.0 24 0.0 25 3.0 26 4.0 27 10.0 28 13.0 29 24.0 30 39.0 31 38.0 32 69.0 33 96.0 34 135.0 35 407.0 36 3096.0 37 63.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.35 11.725 14.799999999999999 37.125 2 27.881970492623154 8.352088022005502 26.331582895723933 37.43435858964741 3 22.225 11.05 29.599999999999998 37.125 4 27.975 14.825 25.874999999999996 31.324999999999996 5 28.525 17.75 27.6 26.125 6 24.375 27.925 22.425 25.275 7 17.45 28.9 35.025 18.625 8 16.625 27.725 35.275 20.375 9 15.4 26.5 37.65 20.45 10-14 20.015 31.3 27.384999999999998 21.3 15-19 18.970000000000002 35.265 25.314999999999998 20.45 20-24 18.69 28.89 29.43 22.99 25-29 21.490000000000002 31.574999999999996 26.889999999999997 20.044999999999998 30-34 24.135 29.630000000000003 25.535000000000004 20.7 35-39 23.380000000000003 31.4 25.185000000000002 20.035 40-44 19.495 30.669999999999998 25.5 24.335 45-49 19.634999999999998 28.845 27.675 23.845 50-54 20.200000000000003 32.39 26.450000000000003 20.96 55-59 21.595 30.255 23.875 24.275 60-64 20.155 31.595000000000002 24.88 23.369999999999997 65-69 19.765 30.130000000000003 25.525 24.58 70-74 23.01 29.925 22.225 24.84 75-79 22.53 29.735 24.834999999999997 22.900000000000002 80-84 21.98 29.409999999999997 25.39 23.22 85-89 25.330000000000002 27.775 25.295 21.6 90-94 21.75 29.575000000000003 27.025 21.65 95-99 24.715 28.98 23.085 23.22 100-104 21.529999999999998 30.755 24.51 23.205000000000002 105-109 21.61 27.639999999999997 26.97 23.78 110-114 24.22 28.12 24.425 23.235 115-119 19.81 29.99 26.135 24.065 120-124 19.36 29.909999999999997 24.98 25.75 125-129 20.375 32.47 21.685 25.47 130-134 23.015 29.794999999999998 25.295 21.895 135-139 23.775 29.53 23.575 23.119999999999997 140-144 24.66 28.12 27.034999999999997 20.185 145-149 22.830000000000002 29.515 25.88 21.775 150 22.8 26.35 29.45 21.4 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 0.5 19 0.5 20 1.0 21 1.0 22 2.0 23 2.5 24 4.5 25 5.5 26 5.5 27 7.5 28 11.0 29 15.5 30 21.0 31 23.0 32 24.5 33 33.5 34 45.0 35 62.0 36 96.5 37 161.0 38 287.0 39 284.0 40 233.5 41 280.5 42 275.5 43 248.5 44 218.0 45 221.0 46 218.5 47 174.5 48 123.5 49 88.5 50 72.0 51 54.5 52 46.0 53 41.5 54 32.0 55 29.5 56 27.0 57 18.5 58 17.5 59 25.5 60 34.5 61 36.0 62 28.5 63 20.5 64 42.0 65 62.5 66 39.0 67 22.0 68 21.5 69 18.0 70 20.0 71 22.0 72 20.0 73 11.0 74 11.5 75 15.5 76 12.0 77 7.0 78 4.0 79 3.5 80 3.5 81 1.5 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.925 #Duplication Level Percentage of deduplicated Percentage of total 1 85.06061791161518 54.37499999999999 2 8.25185764567853 10.549999999999999 3 2.033633163863903 3.9 4 1.6425498631208446 4.2 5 0.5084082909659757 1.625 6 0.4692999608916699 1.7999999999999998 7 0.35197497066875244 1.575 8 0.19554165037152915 1.0 9 0.11732499022291748 0.675 >10 1.3296832225263981 15.925 >50 0.0 0.0 >100 0.03910833007430582 4.375 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 175 4.375 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 47 1.175 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 46 1.15 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 37 0.9249999999999999 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 35 0.8750000000000001 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 35 0.8750000000000001 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 25 0.625 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 21 0.525 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 21 0.525 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 21 0.525 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 20 0.5 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 19 0.475 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 19 0.475 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 19 0.475 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 17 0.42500000000000004 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 17 0.42500000000000004 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 16 0.4 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 15 0.375 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 15 0.375 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 14 0.35000000000000003 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 14 0.35000000000000003 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 14 0.35000000000000003 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 13 0.325 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 13 0.325 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 13 0.325 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 12 0.3 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 12 0.3 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 12 0.3 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 12 0.3 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 11 0.27499999999999997 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 11 0.27499999999999997 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 11 0.27499999999999997 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 10 0.25 No Hit TGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCT 10 0.25 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 10 0.25 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 9 0.22499999999999998 No Hit TGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGA 9 0.22499999999999998 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 9 0.22499999999999998 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 8 0.2 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 8 0.2 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 8 0.2 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 8 0.2 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 8 0.2 No Hit TCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCG 7 0.17500000000000002 No Hit CTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGG 7 0.17500000000000002 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 7 0.17500000000000002 No Hit CCCTCTTCAAATAGATCTAATGGATAAGCTACATAACAGATCCATTGACT 7 0.17500000000000002 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 7 0.17500000000000002 No Hit ATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATA 7 0.17500000000000002 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 7 0.17500000000000002 No Hit TCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTT 7 0.17500000000000002 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 7 0.17500000000000002 No Hit CGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 6 0.15 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 6 0.15 No Hit GCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCC 6 0.15 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 6 0.15 No Hit ACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 6 0.15 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 6 0.15 No Hit TCCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 6 0.15 No Hit GCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATT 6 0.15 No Hit TGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTG 6 0.15 No Hit GAGCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTA 6 0.15 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 6 0.15 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 6 0.15 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 5 0.125 No Hit CGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 5 0.125 No Hit GCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAA 5 0.125 No Hit TGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATA 5 0.125 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 5 0.125 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 5 0.125 No Hit GGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAG 5 0.125 No Hit CTAAAGTTAAGGATTTATCAATGGGTAATGTTGCTCCAATACCTAACCAA 5 0.125 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 5 0.125 No Hit GTCGTGAATAGCTCCGTGGAATAAAATAGAATTTCCTTATGCATAGAACT 5 0.125 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 5 0.125 No Hit TCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATC 5 0.125 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0125 0.0 0.0 0.0 0.0 96-97 0.025 0.0 0.0 0.0 0.0 98-99 0.025 0.0 0.0 0.0 0.0 100-101 0.025 0.0 0.0 0.0 0.0 102-103 0.025 0.0 0.0 0.0 0.0 104-105 0.025 0.0 0.0 0.0 0.0 106-107 0.037500000000000006 0.0 0.0 0.0 0.0 108-109 0.05 0.0 0.0 0.0 0.0 110-111 0.05 0.0 0.0 0.0 0.0 112-113 0.05 0.0 0.0 0.0 0.0 114-115 0.05 0.0 0.0 0.0 0.0 116-117 0.0625 0.0 0.0 0.0 0.0 118-119 0.1 0.0 0.0 0.0 0.0 120-121 0.1 0.0 0.0 0.0 0.0 122-123 0.1 0.0 0.0 0.0 0.0 124-125 0.1 0.0 0.0 0.0 0.0 126-127 0.125 0.0 0.0 0.0 0.0 128-129 0.125 0.0 0.0 0.0 0.0 130-131 0.125 0.0 0.0 0.0 0.0 132-133 0.125 0.0 0.0 0.0 0.0 134-135 0.125 0.0 0.0 0.0 0.0 136-137 0.125 0.0 0.0 0.0 0.0 138 0.125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTATGT 10 0.006973645 144.0 3 TCTTTTC 15 1.1730364E-4 144.0 5 GCTTTCT 15 1.1730364E-4 144.0 1 CTTTCTT 15 1.1730364E-4 144.0 2 TTTCTTT 20 3.687869E-4 108.0 3 TTTTCTT 20 3.687869E-4 108.0 7 TTCTTTT 20 3.687869E-4 108.0 4 CTTTTCT 25 8.956223E-4 86.399994 6 TTTCTTC 25 8.956223E-4 86.399994 8 TTCTTCA 35 0.0034045284 61.714283 9 AGAAGTA 40 0.005777437 54.0 7 GAAGTAG 40 0.005777437 54.0 8 CCGCAGA 40 0.005777437 54.0 3 CGCAGAA 40 0.005777437 54.0 4 GCCGCAG 40 0.005777437 54.0 2 AAGTAGG 45 0.009205684 48.0 9 TACTGGA 25 5.183459E-4 28.8 90-94 ATATCTA 25 5.183459E-4 28.8 85-89 CAGAAGT 80 0.0021206664 27.0 6 AGGCTCG 35 1.2542517E-4 24.685713 65-69 >>END_MODULE ERR11006603 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006603_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1345 37.0 37.0 37.0 37.0 37.0 2 36.21 37.0 37.0 37.0 37.0 37.0 3 36.1985 37.0 37.0 37.0 37.0 37.0 4 36.2935 37.0 37.0 37.0 37.0 37.0 5 36.26 37.0 37.0 37.0 37.0 37.0 6 36.32 37.0 37.0 37.0 37.0 37.0 7 36.3265 37.0 37.0 37.0 37.0 37.0 8 36.3465 37.0 37.0 37.0 37.0 37.0 9 36.3335 37.0 37.0 37.0 37.0 37.0 10-14 36.2927 37.0 37.0 37.0 37.0 37.0 15-19 36.283 37.0 37.0 37.0 37.0 37.0 20-24 36.2815 37.0 37.0 37.0 37.0 37.0 25-29 36.2114 37.0 37.0 37.0 37.0 37.0 30-34 36.1357 37.0 37.0 37.0 37.0 37.0 35-39 36.1225 37.0 37.0 37.0 37.0 37.0 40-44 36.1036 37.0 37.0 37.0 37.0 37.0 45-49 36.0362 37.0 37.0 37.0 37.0 37.0 50-54 36.027 37.0 37.0 37.0 37.0 37.0 55-59 36.0084 37.0 37.0 37.0 37.0 37.0 60-64 35.8928 37.0 37.0 37.0 37.0 37.0 65-69 35.8629 37.0 37.0 37.0 37.0 37.0 70-74 35.8966 37.0 37.0 37.0 37.0 37.0 75-79 35.90599999999999 37.0 37.0 37.0 37.0 37.0 80-84 35.8221 37.0 37.0 37.0 37.0 37.0 85-89 35.787400000000005 37.0 37.0 37.0 37.0 37.0 90-94 35.652300000000004 37.0 37.0 37.0 37.0 37.0 95-99 35.6298 37.0 37.0 37.0 37.0 37.0 100-104 35.425399999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.364 37.0 37.0 37.0 37.0 37.0 110-114 35.3211 37.0 37.0 37.0 32.2 37.0 115-119 35.2625 37.0 37.0 37.0 32.2 37.0 120-124 35.3255 37.0 37.0 37.0 32.2 37.0 125-129 35.2726 37.0 37.0 37.0 29.8 37.0 130-134 35.0746 37.0 37.0 37.0 27.4 37.0 135-139 35.0698 37.0 37.0 37.0 25.0 37.0 140-144 34.878699999999995 37.0 37.0 37.0 25.0 37.0 145-149 34.8434 37.0 37.0 37.0 25.0 37.0 150 34.3975 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 0.0 22 1.0 23 4.0 24 8.0 25 4.0 26 3.0 27 10.0 28 16.0 29 29.0 30 33.0 31 37.0 32 75.0 33 134.0 34 270.0 35 878.0 36 2422.0 37 74.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.400000000000002 24.175 18.475 29.95 2 25.900000000000002 25.174999999999997 34.625 14.299999999999999 3 20.225 28.549999999999997 33.2 18.025 4 24.099999999999998 28.775000000000002 27.0 20.125 5 24.15 30.075000000000003 28.299999999999997 17.474999999999998 6 19.825 33.35 28.825 18.0 7 18.6 21.6 40.925 18.875 8 21.075 23.3 32.775 22.85 9 22.275 21.05 35.125 21.55 10-14 23.68 26.529999999999998 28.854999999999997 20.935000000000002 15-19 23.685000000000002 25.555 30.014999999999997 20.745 20-24 24.68 23.915 30.75 20.655 25-29 24.495 24.37 30.294999999999998 20.84 30-34 24.759999999999998 24.529999999999998 29.970000000000002 20.74 35-39 23.385 25.205 30.264999999999997 21.145 40-44 23.26 25.52 29.81 21.41 45-49 22.795 27.0 28.835 21.37 50-54 22.84 26.27 28.595 22.295 55-59 23.24 24.86 29.25 22.650000000000002 60-64 22.54 25.230000000000004 30.025000000000002 22.205 65-69 23.549999999999997 25.095 29.54 21.815 70-74 23.98 24.625 30.255 21.14 75-79 23.335 24.64 30.505 21.52 80-84 24.665 25.685000000000002 28.365000000000002 21.285 85-89 24.654999999999998 25.314999999999998 27.634999999999998 22.395 90-94 23.585 24.610000000000003 30.11 21.695 95-99 24.5 24.64 28.694999999999997 22.165000000000003 100-104 24.555 23.135 30.525000000000002 21.785 105-109 25.47 23.29 29.78 21.46 110-114 24.055 23.665 29.904999999999998 22.375 115-119 24.33 24.735 29.385 21.55 120-124 24.560000000000002 25.09 27.98 22.37 125-129 23.01 25.779999999999998 29.24 21.97 130-134 23.335 25.169999999999998 28.93 22.564999999999998 135-139 23.62 25.15 30.130000000000003 21.099999999999998 140-144 23.49 24.72 30.470000000000002 21.32 145-149 23.085 23.865 31.04 22.009999999999998 150 23.025000000000002 24.5 29.675 22.8 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0 20 2.0 21 1.5 22 1.5 23 6.5 24 8.0 25 9.0 26 13.0 27 14.0 28 16.5 29 25.0 30 29.0 31 35.5 32 43.0 33 38.0 34 51.5 35 86.0 36 124.5 37 217.0 38 254.0 39 197.0 40 224.0 41 255.0 42 230.0 43 226.0 44 219.5 45 207.0 46 174.0 47 126.5 48 116.5 49 103.0 50 74.5 51 53.5 52 40.0 53 42.5 54 39.0 55 32.5 56 26.5 57 24.5 58 30.5 59 34.0 60 34.5 61 31.5 62 32.5 63 44.0 64 59.5 65 63.5 66 43.5 67 30.5 68 35.5 69 30.0 70 23.5 71 24.5 72 20.0 73 14.0 74 11.5 75 12.0 76 12.5 77 8.0 78 3.0 79 3.5 80 4.5 81 2.5 82 1.5 83 0.5 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.875 #Duplication Level Percentage of deduplicated Percentage of total 1 83.36314847942755 58.25 2 9.910554561717353 13.850000000000001 3 2.2540250447227193 4.725 4 1.3953488372093024 3.9 5 0.9660107334525939 3.375 6 0.4293381037567084 1.7999999999999998 7 0.4293381037567084 2.1 8 0.3220035778175313 1.7999999999999998 9 0.07155635062611806 0.44999999999999996 >10 0.8228980322003578 8.375 >50 0.03577817531305903 1.375 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 55 1.375 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 32 0.8 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 25 0.625 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 25 0.625 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 20 0.5 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 16 0.4 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 16 0.4 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 16 0.4 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 14 0.35000000000000003 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 14 0.35000000000000003 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 13 0.325 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 13 0.325 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 12 0.3 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 12 0.3 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 12 0.3 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 11 0.27499999999999997 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 11 0.27499999999999997 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 11 0.27499999999999997 No Hit ATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATAT 11 0.27499999999999997 No Hit TATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACC 11 0.27499999999999997 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 10 0.25 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 10 0.25 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 10 0.25 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 10 0.25 No Hit AAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATC 9 0.22499999999999998 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 9 0.22499999999999998 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 8 0.2 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 8 0.2 No Hit GTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTAT 8 0.2 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 8 0.2 No Hit AAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAG 8 0.2 No Hit CTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATT 8 0.2 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 8 0.2 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 8 0.2 No Hit GAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAAT 8 0.2 No Hit GGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTC 7 0.17500000000000002 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 7 0.17500000000000002 No Hit GGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTC 7 0.17500000000000002 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 7 0.17500000000000002 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 7 0.17500000000000002 No Hit CTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAG 7 0.17500000000000002 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 7 0.17500000000000002 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 7 0.17500000000000002 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 7 0.17500000000000002 No Hit AATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTT 7 0.17500000000000002 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 7 0.17500000000000002 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 7 0.17500000000000002 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 6 0.15 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 6 0.15 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 6 0.15 No Hit GACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCC 6 0.15 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 6 0.15 No Hit AGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAG 6 0.15 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 6 0.15 No Hit AGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCT 6 0.15 No Hit GAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCG 6 0.15 No Hit CAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAG 6 0.15 No Hit GCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGC 6 0.15 No Hit CTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAA 6 0.15 No Hit CTTCAATATATAATTGCTCCAGTGAATAACTATAAAAAAATAGAAGGACG 5 0.125 No Hit ATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGC 5 0.125 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 5 0.125 No Hit GGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTG 5 0.125 No Hit TGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGA 5 0.125 No Hit GAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGC 5 0.125 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 5 0.125 No Hit ATTCAATTGCGGCCTTATAATGCAATCTCATTCTCTGGTCCAATCGCTGT 5 0.125 No Hit TGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCA 5 0.125 No Hit CTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGC 5 0.125 No Hit GTAGCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGT 5 0.125 No Hit TCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGGCGGT 5 0.125 No Hit ATTTCACATGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTG 5 0.125 No Hit GGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTA 5 0.125 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 5 0.125 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 5 0.125 No Hit CTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTA 5 0.125 No Hit TTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGG 5 0.125 No Hit CTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGG 5 0.125 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 5 0.125 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 5 0.125 No Hit CAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTA 5 0.125 No Hit CTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACC 5 0.125 No Hit GGAAAGTAGAGTAGGCACAGATCCTCTACAAAAGGAAAAATCTATCTTAT 5 0.125 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 5 0.125 No Hit TCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTG 5 0.125 No Hit TTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.025 0.0 0.0 0.0 0.0 92-93 0.025 0.0 0.0 0.0 0.0 94-95 0.037500000000000006 0.0 0.0 0.0 0.0 96-97 0.05 0.0 0.0 0.0 0.0 98-99 0.05 0.0 0.0 0.0 0.0 100-101 0.05 0.0 0.0 0.0 0.0 102-103 0.05 0.0 0.0 0.0 0.0 104-105 0.05 0.0 0.0 0.0 0.0 106-107 0.0625 0.0 0.0 0.0 0.0 108-109 0.075 0.0 0.0 0.0 0.0 110-111 0.075 0.0 0.0 0.0 0.0 112-113 0.075 0.0 0.0 0.0 0.0 114-115 0.075 0.0 0.0 0.0 0.0 116-117 0.0875 0.0 0.0 0.0 0.0 118-119 0.125 0.0 0.0 0.0 0.0 120-121 0.125 0.0 0.0 0.0 0.0 122-123 0.125 0.0 0.0 0.0 0.0 124-125 0.125 0.0 0.0 0.0 0.0 126-127 0.15 0.0 0.0 0.0 0.0 128-129 0.15 0.0 0.0 0.0 0.0 130-131 0.15 0.0 0.0 0.0 0.0 132-133 0.15 0.0 0.0 0.0 0.0 134-135 0.15 0.0 0.0 0.0 0.0 136-137 0.15 0.0 0.0 0.0 0.0 138 0.15 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 2746017 spots for ERR11006603.sra Written 2746017 spots for ERR11006603.sra Read 2746017 spots for ERR11006603.sra Written 2746017 spots for ERR11006603.sra Read 2746017 spots for ERR11006603.sra Written 2746017 spots for ERR11006603.sra Read 2746017 spots for ERR11006603.sra Written 2746017 spots for ERR11006603.sra Read 2746017 spots for ERR11006603.sra Written 2746017 spots for ERR11006603.sra Read 2746017 spots for ERR11006603.sra Written 2746017 spots for ERR11006603.sra Read 2746017 spots for ERR11006603.sra Written 2746017 spots for ERR11006603.sra Read 2746017 spots for ERR11006603.sra Written 2746017 spots for ERR11006603.sra Read 2746017 spots for ERR11006603.sra Written 2746017 spots for ERR11006603.sra Read 2746017 spots for ERR11006603.sra Written 2746017 spots for ERR11006603.sra Read 2746017 spots for ERR11006603.sra Written 2746017 spots for ERR11006603.sra Read 2746017 spots for ERR11006603.sra Written 2746017 spots for ERR11006603.sra Read 2746017 spots for ERR11006603.sra Written 2746017 spots for ERR11006603.sra Read 2746017 spots for ERR11006603.sra Written 2746017 spots for ERR11006603.sra Read 2746017 spots for ERR11006603.sra Written 2746017 spots for ERR11006603.sra Read 2746017 spots for ERR11006603.sra Written 2746017 spots for ERR11006603.sra Read 2746018 spots for ERR11006603.sra Written 2746018 spots for ERR11006603.sra Read 2746017 spots for ERR11006603.sra Written 2746017 spots for ERR11006603.sra Read 2746017 spots for ERR11006603.sra Written 2746017 spots for ERR11006603.sra Read 2746017 spots for ERR11006603.sra Written 2746017 spots for ERR11006603.sra SRR ids: ['ERR11006603.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_03z5xwu9 ERR11006603.sra spots: 54920341 blocks: [[1, 2746017], [2746018, 5492034], [5492035, 8238051], [8238052, 10984068], [10984069, 13730085], [13730086, 16476102], [16476103, 19222119], [19222120, 21968136], [21968137, 24714153], [24714154, 27460170], [27460171, 30206187], [30206188, 32952204], [32952205, 35698221], [35698222, 38444238], [38444239, 41190255], [41190256, 43936272], [43936273, 46682289], [46682290, 49428306], [49428307, 52174323], [52174324, 54920341]] ERR11006603 file size 20181016 ERR11006603 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006603 ERR11006603_1.fastq ERR11006603_2.fastq Input file: ERR11006603_1.fastq Paired file: ERR11006603_2.fastq trimmed: ERR11006603-trimmed-pair1.fastq, ERR11006603-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 19:32:12 2024 >> started Fri Dec 6 19:33:15 2024 >> done (63.782s) 54920341 read pairs processed; of these: 554 ( 0.00%) short read pairs filtered out after trimming by size control 860 ( 0.00%) empty read pairs filtered out after trimming by size control 54918927 (100.00%) read pairs available; of these: 141654 ( 0.26%) trimmed read pairs available after processing 54777273 (99.74%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 19 0.00% 19 38 0.00% 20 18 0.00% 21 50 0.00% 22 69 0.00% 23 22 0.00% 24 21 0.00% 25 26 0.00% 26 35 0.00% 27 24 0.00% 28 35 0.00% 29 26 0.00% 30 31 0.00% 31 56 0.00% 32 36 0.00% 33 25 0.00% 34 26 0.00% 35 28 0.00% 36 36 0.00% 37 31 0.00% 38 29 0.00% 39 26 0.00% 40 40 0.00% 41 54 0.00% 42 40 0.00% 43 48 0.00% 44 50 0.00% 45 42 0.00% 46 49 0.00% 47 45 0.00% 48 47 0.00% 49 55 0.00% 50 57 0.00% 51 48 0.00% 52 46 0.00% 53 45 0.00% 54 63 0.00% 55 85 0.00% 56 75 0.00% 57 71 0.00% 58 70 0.00% 59 83 0.00% 60 89 0.00% 61 83 0.00% 62 85 0.00% 63 97 0.00% 64 118 0.00% 65 111 0.00% 66 114 0.00% 67 103 0.00% 68 144 0.00% 69 120 0.00% 70 147 0.00% 71 154 0.00% 72 156 0.00% 73 183 0.00% 74 168 0.00% 75 177 0.00% 76 173 0.00% 77 182 0.00% 78 201 0.00% 79 225 0.00% 80 225 0.00% 81 254 0.00% 82 235 0.00% 83 220 0.00% 84 260 0.00% 85 279 0.00% 86 316 0.00% 87 371 0.00% 88 359 0.00% 89 371 0.00% 90 407 0.00% 91 406 0.00% 92 419 0.00% 93 412 0.00% 94 459 0.00% 95 504 0.00% 96 473 0.00% 97 611 0.00% 98 628 0.00% 99 647 0.00% 100 666 0.00% 101 716 0.00% 102 759 0.00% 103 798 0.00% 104 784 0.00% 105 821 0.00% 106 932 0.00% 107 909 0.00% 108 1023 0.00% 109 1023 0.00% 110 1140 0.00% 111 1125 0.00% 112 1336 0.00% 113 1321 0.00% 114 1326 0.00% 115 1345 0.00% 116 1437 0.00% 117 1627 0.00% 118 1656 0.00% 119 1730 0.00% 120 1794 0.00% 121 1937 0.00% 122 2188 0.00% 123 2073 0.00% 124 2225 0.00% 125 2583 0.00% 126 2309 0.00% 127 2380 0.00% 128 2479 0.00% 129 2685 0.00% 130 2997 0.01% 131 3069 0.01% 132 3217 0.01% 133 3067 0.01% 134 3273 0.01% 135 3016 0.01% 136 3347 0.01% 137 3286 0.01% 138 3506 0.01% 139 3799 0.01% 140 3824 0.01% 141 4180 0.01% 142 4416 0.01% 143 4701 0.01% 144 4744 0.01% 145 4997 0.01% 146 6414 0.01% 147 5891 0.01% 148 5767 0.01% 149 6510 0.01% 150 54777273 99.74% 54918927 reads passed initial QC criterion=sequence-density sequence-density=0.28 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=31 prefix-density=0.27 prefix-fanout=2.0 sequence=TCTAATTCAAAA criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=107.63 fanout-score-rank=1 prefix-density=0.57 prefix-fanout=1.1 sequence=CAGTAGAAGTAGAACAGGTATAAATAAGAAAATCTTAGTTAAGAGGGTTCATGTAAAGAACAGGTTCTAAATCACGATCGATTCCCTTTTCAAAACCTGCTGCAGCAGCTCGGGCTCTTCCTGCATGCCACAAATGGCCCACAAAAAAGAAGAATCCTAGAACAAAATGAGAAGTCGATAACCAACTTCTAGGAGAGACATAATTAACTGCATTGATCTCGGTAGCTACGCCACCCACGGAATTTAAAGAGCCTAAAGGAGCATGGGTCATATATTCCGCTGAACGTCGTTCTTGCCAAGGTTGTATGTCTTTTTTCAACCTACTCAAGTCCAAACCGTTGGGCCCCCTTAGAGGTTCTAACCATGGAGCACGGAGGTCCCAAAAACGCATAGTTTCCCCTCCAAAGATAACCTCTCCCGTTGGGGAACGCATTAGATATTTACCTAAACCTGTGGGTCCTTGAGCAGATCCCACATTAGCTCCAAGACGCTGGTCTCTAACTAGAAAAGT criterion=sequence-density sequence-density=0.30 sequence-density-rank=1 fanout-score=3.48 fanout-score-rank=23 prefix-density=0.49 prefix-fanout=2.1 sequence=GGGGAAGAGGACTGAAACATGCCACTGAAAGACTCTACTGAGACAAAAAGATGGGCTGTCAAAAAGGTAGAGGAGGTAGGATGGGCAGTTGGTCAGATCTAGTATGGATCGTACATGGACGATAGTTGGAGTCGGCGGCTCTCCTAGGCTTCCCTCATCTGGGATCCCTGGGGAAGAGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=43 fanout-score=714.58 fanout-score-rank=1 prefix-density=3.91 prefix-fanout=1.0 sequence=ACTTCTTACTTCCATTAGTCCCCGTGTTCTTCGAATGGATCTCTTAATTGTTGAGAGGGTTGCCCAAACGCGGTATATAAGGCATACCCAGTAAAGCTTACAAGTAAACCAGATATGGAGATGGCGACTAAAGTTGCTGTTTCCATTTTTATAGAATTTAAAGATTACAATGGATCTACAAAAAGATCGT ERR11006603 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 19:34:19 Started mapping on | Dec 06 19:34:19 Finished on | Dec 06 19:40:01 Mapping speed, Million of reads per hour | 578.09 Number of input reads | 54918927 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 41845598 Uniquely mapped reads % | 76.20% Average mapped length | 298.65 Number of splices: Total | 13648991 Number of splices: Annotated (sjdb) | 12653584 Number of splices: GT/AG | 13267442 Number of splices: GC/AG | 168237 Number of splices: AT/AC | 19502 Number of splices: Non-canonical | 193810 Mismatch rate per base, % | 0.31% Deletion rate per base | 0.01% Deletion average length | 1.81 Insertion rate per base | 0.01% Insertion average length | 2.01 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 11408058 % of reads mapped to multiple loci | 20.77% Number of reads mapped to too many loci | 6099 % of reads mapped to too many loci | 0.01% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.89% % of reads unmapped: other | 0.14% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1665271 1665271 1665271 N_multimapping 11408058 11408058 11408058 N_noFeature 9954638 38356461 12210472 N_ambiguous 2105907 63559 899515 UnstrandedReadsAssigned:29785053 PositiveStrandReadsAssigned:3425578 NegativeStrandReadsAssigned:28735611 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006603 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006603-trimmed-pair1.fastq ERR11006603-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 54,918,927 reads, 32,372,179 reads pseudoaligned [quant] estimated average fragment length: 316.532 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,094 rounds 52973 ERR11006603.ke.tsv 35125 ERR11006603.se.tsv 88098 total ==> ERR11006603.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 620.966 0 0 PNS24247 1044 728.468 11.5494 0.40701 PNS24249 1928 1612.47 136.603 2.17484 PNS24246 1044 728.468 11.5494 0.40701 PNS24248 1044 728.468 11.5494 0.40701 PNS24244 1471 1155.47 30.7486 0.683165 PNS24243 293 42.1714 0 0 KQK14069 1603 1287.47 3598.66 71.7565 KQK14071 474 164.366 14.5574 2.27369 ==> ERR11006603.se.tsv <== BRADI_1g14170v3 3889 BRADI_1g53295v3 46 BRADI_1g59795v3 193 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 178 BRADI_1g74790v3 182 BRADI_1g09890v3 0 BRADI_1g77505v3 213 BRADI_1g48960v3 0 ERR11006603 completed mapping pipeline successfully