Starting /dee2/code/volunteer_pipeline.sh ERR11006604 current disk space = 1549839245312 free memory = 1603760096 ERR11006604 SRAfilesize d0ebd19911780699902cb5196c57c9d6 ERR11006604.sra ERR11006604.sra file validated ERR11006604 is paired end ERR11006604 is conventional basespace ERR11006604 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006604_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.18675 37.0 37.0 37.0 37.0 37.0 2 36.3385 37.0 37.0 37.0 37.0 37.0 3 36.3735 37.0 37.0 37.0 37.0 37.0 4 36.4785 37.0 37.0 37.0 37.0 37.0 5 36.314 37.0 37.0 37.0 37.0 37.0 6 36.464 37.0 37.0 37.0 37.0 37.0 7 36.436 37.0 37.0 37.0 37.0 37.0 8 36.425 37.0 37.0 37.0 37.0 37.0 9 36.357 37.0 37.0 37.0 37.0 37.0 10-14 36.531099999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.4324 37.0 37.0 37.0 37.0 37.0 20-24 36.4041 37.0 37.0 37.0 37.0 37.0 25-29 36.3651 37.0 37.0 37.0 37.0 37.0 30-34 36.2719 37.0 37.0 37.0 37.0 37.0 35-39 36.213499999999996 37.0 37.0 37.0 37.0 37.0 40-44 36.2126 37.0 37.0 37.0 37.0 37.0 45-49 36.2122 37.0 37.0 37.0 37.0 37.0 50-54 36.2186 37.0 37.0 37.0 37.0 37.0 55-59 36.224900000000005 37.0 37.0 37.0 37.0 37.0 60-64 35.954899999999995 37.0 37.0 37.0 37.0 37.0 65-69 36.15990000000001 37.0 37.0 37.0 37.0 37.0 70-74 36.1785 37.0 37.0 37.0 37.0 37.0 75-79 36.133300000000006 37.0 37.0 37.0 37.0 37.0 80-84 36.0697 37.0 37.0 37.0 37.0 37.0 85-89 36.0002 37.0 37.0 37.0 37.0 37.0 90-94 35.775800000000004 37.0 37.0 37.0 37.0 37.0 95-99 36.0267 37.0 37.0 37.0 37.0 37.0 100-104 36.001400000000004 37.0 37.0 37.0 37.0 37.0 105-109 35.866099999999996 37.0 37.0 37.0 37.0 37.0 110-114 35.7735 37.0 37.0 37.0 37.0 37.0 115-119 35.8309 37.0 37.0 37.0 37.0 37.0 120-124 35.7014 37.0 37.0 37.0 37.0 37.0 125-129 35.794799999999995 37.0 37.0 37.0 37.0 37.0 130-134 35.63719999999999 37.0 37.0 37.0 37.0 37.0 135-139 35.50105 37.0 37.0 37.0 37.0 37.0 140-144 35.675 37.0 37.0 37.0 37.0 37.0 145-149 35.5003 37.0 37.0 37.0 34.6 37.0 150 35.427 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 3.0 26 6.0 27 13.0 28 16.0 29 23.0 30 37.0 31 30.0 32 81.0 33 84.0 34 136.0 35 402.0 36 3086.0 37 82.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.36363636363637 12.021036814425244 14.400200350613574 37.215126471324815 2 27.952952952952952 9.184184184184184 26.826826826826828 36.03603603603604 3 20.325 12.2 29.425 38.05 4 25.900000000000002 14.875 28.225 31.0 5 27.55 17.825 28.749999999999996 25.874999999999996 6 24.3 29.025000000000002 21.05 25.624999999999996 7 18.025 27.775 34.725 19.475 8 15.9 28.849999999999998 36.5 18.75 9 16.25 27.200000000000003 38.675 17.875 10-14 19.08 31.34 27.96 21.62 15-19 18.855 37.035000000000004 24.385 19.725 20-24 17.580000000000002 29.635 29.9 22.884999999999998 25-29 21.310000000000002 32.6 27.084999999999997 19.005 30-34 23.705000000000002 30.525000000000002 26.085 19.685 35-39 22.395 32.615 25.264999999999997 19.725 40-44 18.995392628205128 30.21834935897436 26.542467948717945 24.243790064102562 45-49 18.48 30.535 27.705000000000002 23.28 50-54 19.13 33.605000000000004 26.545 20.72 55-59 22.23 30.020000000000003 23.810000000000002 23.94 60-64 20.23 32.75 24.21 22.81 65-69 19.61 30.775000000000002 25.495 24.12 70-74 23.02 30.735 21.8 24.445 75-79 21.7 30.464999999999996 25.564999999999998 22.27 80-84 21.529999999999998 29.865000000000002 25.685000000000002 22.919999999999998 85-89 26.095000000000002 28.89 24.085 20.93 90-94 21.465 30.14 27.334999999999997 21.060000000000002 95-99 24.91 29.549999999999997 23.51 22.03 100-104 20.97 30.98 25.740000000000002 22.31 105-109 21.415 28.785 27.12 22.68 110-114 23.84 27.939999999999998 25.21 23.01 115-119 18.87 30.654999999999998 26.889999999999997 23.585 120-124 19.09 30.3 25.355 25.255 125-129 19.564999999999998 33.01 22.355 25.069999999999997 130-134 22.91 30.81 25.224999999999998 21.055 135-139 23.510846149992485 29.818145383497818 22.65417564250288 24.016832824006816 140-144 25.165 29.13 26.46 19.245 145-149 22.89 31.009999999999998 25.985000000000003 20.115 150 21.45 26.674999999999997 30.425 21.45 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 1.5 19 2.5 20 1.0 21 3.0 22 5.5 23 4.0 24 5.0 25 5.0 26 4.0 27 8.5 28 14.5 29 16.5 30 16.0 31 21.5 32 30.0 33 32.5 34 46.5 35 64.5 36 89.0 37 178.5 38 337.0 39 340.5 40 264.0 41 277.5 42 273.0 43 254.5 44 222.5 45 211.0 46 203.0 47 158.5 48 123.0 49 95.5 50 65.0 51 48.5 52 39.5 53 32.5 54 26.0 55 24.0 56 22.0 57 16.5 58 20.5 59 23.5 60 22.0 61 24.0 62 23.5 63 22.5 64 38.0 65 55.0 66 39.0 67 20.0 68 16.0 69 14.0 70 15.5 71 12.0 72 10.0 73 8.5 74 7.5 75 11.0 76 8.0 77 5.0 78 5.0 79 4.0 80 4.0 81 3.0 82 1.0 83 1.0 84 1.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17500000000000002 2 0.1 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.16 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.19499999999999998 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.25 #Duplication Level Percentage of deduplicated Percentage of total 1 81.05485232067511 48.025 2 11.518987341772153 13.65 3 2.3628691983122363 4.2 4 1.4345991561181435 3.4000000000000004 5 0.7594936708860759 2.25 6 0.6329113924050633 2.25 7 0.42194092827004215 1.7500000000000002 8 0.21097046413502107 1.0 9 0.08438818565400844 0.44999999999999996 >10 1.350210970464135 13.725000000000001 >50 0.12658227848101267 4.625 >100 0.04219409282700422 4.675 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 187 4.675 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 62 1.55 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 62 1.55 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 61 1.525 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 38 0.95 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 35 0.8750000000000001 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 30 0.75 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 25 0.625 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 24 0.6 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 24 0.6 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 23 0.575 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 21 0.525 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 19 0.475 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 19 0.475 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 19 0.475 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 17 0.42500000000000004 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 17 0.42500000000000004 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 16 0.4 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 16 0.4 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 16 0.4 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 16 0.4 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 14 0.35000000000000003 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 14 0.35000000000000003 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 13 0.325 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 13 0.325 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 12 0.3 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 12 0.3 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 12 0.3 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 11 0.27499999999999997 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 11 0.27499999999999997 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 11 0.27499999999999997 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 11 0.27499999999999997 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 10 0.25 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 10 0.25 No Hit CCTCCACCAAATTGTAATACAGAATCATCCCCAAAGATTTCGGTCAGAGC 10 0.25 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 10 0.25 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 9 0.22499999999999998 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 9 0.22499999999999998 No Hit CCGCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGAT 8 0.2 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 8 0.2 No Hit ACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 8 0.2 No Hit GTCGTGAATAGCTCCGTGGAATAAAATAGAATTTCCTTATGCATAGAACT 8 0.2 No Hit GGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGG 8 0.2 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 7 0.17500000000000002 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 7 0.17500000000000002 No Hit CCCCTTCTAACTTACCTACTACTGTACCGGCGTGGATATGATCTCCCCCA 7 0.17500000000000002 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 7 0.17500000000000002 No Hit GCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCC 7 0.17500000000000002 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 7 0.17500000000000002 No Hit TCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGT 7 0.17500000000000002 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 7 0.17500000000000002 No Hit GGCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGAT 7 0.17500000000000002 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 7 0.17500000000000002 No Hit GAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATA 6 0.15 No Hit AGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGA 6 0.15 No Hit TGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATT 6 0.15 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 6 0.15 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 6 0.15 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 6 0.15 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 6 0.15 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 6 0.15 No Hit ATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATA 6 0.15 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 6 0.15 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 6 0.15 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 6 0.15 No Hit CCTAAAGTTAAGGATTTATCAATGGGTAATGTTGCTCCAATACCTAACCA 6 0.15 No Hit CCCAGGAACAGGCTCGATGTGATAGCATCGTCCTTTGTAACGATCAAGAC 6 0.15 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 6 0.15 No Hit TCTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTA 5 0.125 No Hit GGGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACA 5 0.125 No Hit TGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAA 5 0.125 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 5 0.125 No Hit CTCCACCAAATTGTAATACAGAATCATCCCCAAAGATTTCGGTCAGAGCT 5 0.125 No Hit GCCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 5 0.125 No Hit ATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTT 5 0.125 No Hit CCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAA 5 0.125 No Hit CTGTAAACCACCCTCCTAAAGCGAAATAAGCACAAGGAAAGAGCAATAAG 5 0.125 No Hit GGTCGAACTACCAGAATGTCTAGAAATGTAGAGCTTAAACTAGAAAGGCT 5 0.125 No Hit ACCCTCTTCAAATAGATCTAATGGATAAGCTACATAACAGATCCATTGAC 5 0.125 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 5 0.125 No Hit GGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAG 5 0.125 No Hit TTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAG 5 0.125 No Hit TGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTG 5 0.125 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 5 0.125 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 5 0.125 No Hit CCCCCAGTTAAGTAGTCATGCATTACAATAGGAACACCTAATTCTCTCGC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.025 0.0 0.0 0.0 0.0 92-93 0.025 0.0 0.0 0.0 0.0 94-95 0.025 0.0 0.0 0.0 0.0 96-97 0.025 0.0 0.0 0.0 0.0 98-99 0.025 0.0 0.0 0.0 0.0 100-101 0.025 0.0 0.0 0.0 0.0 102-103 0.025 0.0 0.0 0.0 0.0 104-105 0.025 0.0 0.0 0.0 0.0 106-107 0.025 0.0 0.0 0.0 0.0 108-109 0.025 0.0 0.0 0.0 0.0 110-111 0.025 0.0 0.0 0.0 0.0 112-113 0.025 0.0 0.0 0.0 0.0 114-115 0.025 0.0 0.0 0.0 0.0 116-117 0.025 0.0 0.0 0.0 0.0 118-119 0.025 0.0 0.0 0.0 0.0 120-121 0.025 0.0 0.0 0.0 0.0 122-123 0.025 0.0 0.0 0.0 0.0 124-125 0.025 0.0 0.0 0.0 0.0 126-127 0.025 0.0 0.0 0.0 0.0 128-129 0.037500000000000006 0.0 0.0 0.0 0.0 130-131 0.05 0.0 0.0 0.0 0.0 132-133 0.0625 0.0 0.0 0.0 0.0 134-135 0.075 0.0 0.0 0.0 0.0 136-137 0.075 0.0 0.0 0.0 0.0 138 0.075 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGGAAG 10 0.006973645 144.0 4 CCACTTG 10 0.006973645 144.0 4 CGCGGAA 10 0.006973645 144.0 3 GCTTTCT 35 1.5315891E-9 123.428566 1 CTTTTCT 50 1.828812E-8 86.399994 6 CTTTCTT 50 1.828812E-8 86.399994 2 TTTCTTT 55 3.543937E-8 78.545456 3 TTTCTTC 55 3.543937E-8 78.545456 8 TTCTTTT 55 3.543937E-8 78.545456 4 TCTTTTC 55 3.543937E-8 78.545456 5 TTCTTCA 65 1.1285738E-7 66.46153 9 TTTTCTT 70 1.8854553E-7 61.714283 7 TATAAAT 20 0.006139246 28.8 135-139 TCTTATA 45 2.7146598E-8 28.8 20-24 TAGCGGA 50 7.5498974E-8 25.919998 30-34 TATGTTA 50 7.5498974E-8 25.919998 25-29 ACCTTAT 50 7.5498974E-8 25.919998 40-44 TTAATAG 50 7.5498974E-8 25.919998 50-54 AATTCTT 45 8.905736E-7 25.6 15-19 TTCAAAA 45 8.905736E-7 25.6 10-14 >>END_MODULE ERR11006604 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006604_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.542 37.0 37.0 37.0 37.0 37.0 2 35.958 37.0 37.0 37.0 37.0 37.0 3 36.033 37.0 37.0 37.0 37.0 37.0 4 35.987 37.0 37.0 37.0 37.0 37.0 5 36.066 37.0 37.0 37.0 37.0 37.0 6 36.1645 37.0 37.0 37.0 37.0 37.0 7 36.117 37.0 37.0 37.0 37.0 37.0 8 36.157 37.0 37.0 37.0 37.0 37.0 9 36.17 37.0 37.0 37.0 37.0 37.0 10-14 36.141000000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.1558 37.0 37.0 37.0 37.0 37.0 20-24 36.1423 37.0 37.0 37.0 37.0 37.0 25-29 36.0201 37.0 37.0 37.0 37.0 37.0 30-34 35.985 37.0 37.0 37.0 37.0 37.0 35-39 35.8712 37.0 37.0 37.0 37.0 37.0 40-44 36.0005 37.0 37.0 37.0 37.0 37.0 45-49 35.92999999999999 37.0 37.0 37.0 37.0 37.0 50-54 35.8498 37.0 37.0 37.0 37.0 37.0 55-59 35.8477 37.0 37.0 37.0 37.0 37.0 60-64 35.8256 37.0 37.0 37.0 37.0 37.0 65-69 35.7448 37.0 37.0 37.0 37.0 37.0 70-74 35.7411 37.0 37.0 37.0 37.0 37.0 75-79 35.803 37.0 37.0 37.0 37.0 37.0 80-84 35.737700000000004 37.0 37.0 37.0 37.0 37.0 85-89 35.6802 37.0 37.0 37.0 37.0 37.0 90-94 35.458800000000004 37.0 37.0 37.0 34.6 37.0 95-99 35.4906 37.0 37.0 37.0 37.0 37.0 100-104 35.4097 37.0 37.0 37.0 37.0 37.0 105-109 35.3409 37.0 37.0 37.0 29.8 37.0 110-114 35.2256 37.0 37.0 37.0 27.4 37.0 115-119 35.1646 37.0 37.0 37.0 27.4 37.0 120-124 35.2615 37.0 37.0 37.0 29.8 37.0 125-129 35.09779999999999 37.0 37.0 37.0 25.0 37.0 130-134 35.0299 37.0 37.0 37.0 25.0 37.0 135-139 35.001599999999996 37.0 37.0 37.0 25.0 37.0 140-144 34.8418 37.0 37.0 37.0 25.0 37.0 145-149 34.764799999999994 37.0 37.0 37.0 25.0 37.0 150 34.355 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 1.0 20 0.0 21 2.0 22 2.0 23 3.0 24 6.0 25 10.0 26 17.0 27 12.0 28 19.0 29 33.0 30 31.0 31 51.0 32 79.0 33 112.0 34 287.0 35 994.0 36 2276.0 37 64.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.08616187989556 27.04960835509138 18.067885117493475 25.796344647519582 2 25.15 26.125 34.225 14.499999999999998 3 20.0 27.625 33.275 19.1 4 23.175 30.725 27.725 18.375 5 23.25 29.099999999999998 29.7 17.95 6 20.150000000000002 33.375 28.599999999999998 17.875 7 19.25 21.55 41.525 17.675 8 20.674999999999997 22.675 33.175 23.474999999999998 9 23.5 20.375 35.5 20.625 10-14 22.96 27.250000000000004 29.465000000000003 20.325 15-19 23.285 26.029999999999998 31.019999999999996 19.665 20-24 24.055 24.72 30.659999999999997 20.565 25-29 24.03 24.81 31.080000000000002 20.080000000000002 30-34 23.95 24.13 31.235000000000003 20.685000000000002 35-39 23.41 25.22 30.97 20.4 40-44 22.75 25.814999999999998 30.669999999999998 20.765 45-49 21.36 27.825 30.2 20.615 50-54 22.18 26.965 29.294999999999998 21.560000000000002 55-59 22.435 25.845000000000002 29.759999999999998 21.959999999999997 60-64 21.6 26.445 29.770000000000003 22.185 65-69 23.080000000000002 25.369999999999997 29.62 21.93 70-74 23.205000000000002 25.924999999999997 29.95 20.919999999999998 75-79 23.345 25.419999999999998 31.019999999999996 20.215 80-84 24.25 25.074999999999996 30.39 20.285 85-89 24.185000000000002 25.540000000000003 28.294999999999998 21.98 90-94 23.244999999999997 25.509999999999998 29.520000000000003 21.725 95-99 23.605 24.9 29.215000000000003 22.28 100-104 24.59 23.400000000000002 31.269999999999996 20.74 105-109 25.035 23.465 30.575000000000003 20.925 110-114 23.48 24.2 30.495 21.825 115-119 23.25 25.405 29.65 21.695 120-124 23.849999999999998 25.535000000000004 28.895 21.72 125-129 22.6 25.779999999999998 30.435000000000002 21.185000000000002 130-134 22.735 26.685 29.225 21.355 135-139 22.93 26.99 29.909999999999997 20.169999999999998 140-144 23.09 24.855 31.455 20.599999999999998 145-149 22.455 24.415 31.615 21.515 150 21.575 24.575 31.15 22.7 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0 17 1.5 18 1.0 19 1.0 20 0.5 21 1.0 22 2.0 23 4.5 24 8.0 25 12.5 26 12.0 27 10.0 28 15.5 29 17.0 30 25.0 31 39.5 32 40.5 33 48.0 34 67.0 35 93.5 36 142.0 37 250.0 38 281.5 39 223.5 40 231.5 41 273.0 42 249.5 43 231.5 44 235.0 45 200.5 46 176.0 47 144.5 48 113.5 49 78.0 50 57.0 51 53.0 52 38.0 53 28.0 54 26.0 55 27.0 56 24.5 57 26.5 58 35.5 59 31.5 60 24.0 61 22.5 62 24.5 63 41.0 64 49.0 65 40.5 66 27.5 67 16.5 68 25.0 69 28.0 70 19.5 71 20.0 72 18.5 73 11.5 74 11.0 75 10.5 76 7.5 77 5.5 78 5.0 79 5.0 80 3.0 81 1.5 82 1.5 83 0.5 84 0.0 85 1.0 86 1.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.25 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.27499999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 80.82497212931996 54.37499999999999 2 11.445559271646228 15.4 3 3.0471943515421778 6.15 4 1.4121144555927163 3.8 5 0.966183574879227 3.25 6 0.5574136008918618 2.25 7 0.4087699739873653 1.925 8 0.29728725380899296 1.6 9 0.2229654403567447 1.35 >10 0.7803790412486065 7.85 >50 0.03716090672612412 2.0500000000000003 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 82 2.0500000000000003 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 30 0.75 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 23 0.575 No Hit ATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATAT 20 0.5 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 20 0.5 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 18 0.44999999999999996 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 17 0.42500000000000004 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 15 0.375 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 14 0.35000000000000003 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 14 0.35000000000000003 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 14 0.35000000000000003 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 13 0.325 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 13 0.325 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 13 0.325 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 12 0.3 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 12 0.3 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 12 0.3 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 12 0.3 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 11 0.27499999999999997 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 11 0.27499999999999997 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 10 0.25 No Hit TATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACC 10 0.25 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 9 0.22499999999999998 No Hit AAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATC 9 0.22499999999999998 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 9 0.22499999999999998 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 9 0.22499999999999998 No Hit AATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTAT 9 0.22499999999999998 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 9 0.22499999999999998 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 8 0.2 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 8 0.2 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 8 0.2 No Hit AGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCT 8 0.2 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 8 0.2 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 8 0.2 No Hit TCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTG 8 0.2 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 8 0.2 No Hit ATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAGC 7 0.17500000000000002 No Hit CTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCT 7 0.17500000000000002 No Hit GTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCT 7 0.17500000000000002 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 7 0.17500000000000002 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 7 0.17500000000000002 No Hit GCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGC 7 0.17500000000000002 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 7 0.17500000000000002 No Hit TCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGA 7 0.17500000000000002 No Hit AATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTA 7 0.17500000000000002 No Hit TACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGG 7 0.17500000000000002 No Hit CTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTC 7 0.17500000000000002 No Hit TGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATAC 6 0.15 No Hit ATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCT 6 0.15 No Hit AAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAG 6 0.15 No Hit GGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTC 6 0.15 No Hit AATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTA 6 0.15 No Hit CTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAG 6 0.15 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 6 0.15 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 6 0.15 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 6 0.15 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 6 0.15 No Hit TTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGG 6 0.15 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 6 0.15 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 6 0.15 No Hit TATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCAT 6 0.15 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 6 0.15 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 5 0.125 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 5 0.125 No Hit CCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTG 5 0.125 No Hit ACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAG 5 0.125 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 5 0.125 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 5 0.125 No Hit GACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCC 5 0.125 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 5 0.125 No Hit GTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCAT 5 0.125 No Hit TCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGA 5 0.125 No Hit AGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAG 5 0.125 No Hit GGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTT 5 0.125 No Hit GTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGT 5 0.125 No Hit AATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGC 5 0.125 No Hit GTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACT 5 0.125 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 5 0.125 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 5 0.125 No Hit GTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATGTCA 5 0.125 No Hit AGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACT 5 0.125 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 5 0.125 No Hit GGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTT 5 0.125 No Hit ACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCG 5 0.125 No Hit CTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTAC 5 0.125 No Hit CTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAA 5 0.125 No Hit GGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACA 5 0.125 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.025 0.0 0.0 0.0 0.0 92-93 0.025 0.0 0.0 0.0 0.0 94-95 0.025 0.0 0.0 0.0 0.0 96-97 0.025 0.0 0.0 0.0 0.0 98-99 0.025 0.0 0.0 0.0 0.0 100-101 0.025 0.0 0.0 0.0 0.0 102-103 0.025 0.0 0.0 0.0 0.0 104-105 0.025 0.0 0.0 0.0 0.0 106-107 0.025 0.0 0.0 0.0 0.0 108-109 0.025 0.0 0.0 0.0 0.0 110-111 0.025 0.0 0.0 0.0 0.0 112-113 0.025 0.0 0.0 0.0 0.0 114-115 0.025 0.0 0.0 0.0 0.0 116-117 0.025 0.0 0.0 0.0 0.0 118-119 0.025 0.0 0.0 0.0 0.0 120-121 0.025 0.0 0.0 0.0 0.0 122-123 0.025 0.0 0.0 0.0 0.0 124-125 0.025 0.0 0.0 0.0 0.0 126-127 0.025 0.0 0.0 0.0 0.0 128-129 0.037500000000000006 0.0 0.0 0.0 0.0 130-131 0.05 0.0 0.0 0.0 0.0 132-133 0.0625 0.0 0.0 0.0 0.0 134-135 0.075 0.0 0.0 0.0 0.0 136-137 0.075 0.0 0.0 0.0 0.0 138 0.075 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 2645802 spots for ERR11006604.sra Written 2645802 spots for ERR11006604.sra Read 2645802 spots for ERR11006604.sra Written 2645802 spots for ERR11006604.sra Read 2645802 spots for ERR11006604.sra Written 2645802 spots for ERR11006604.sra Read 2645802 spots for ERR11006604.sra Written 2645802 spots for ERR11006604.sra Read 2645802 spots for ERR11006604.sra Written 2645802 spots for ERR11006604.sra Read 2645802 spots for ERR11006604.sra Written 2645802 spots for ERR11006604.sra Read 2645802 spots for ERR11006604.sra Written 2645802 spots for ERR11006604.sra Read 2645802 spots for ERR11006604.sra Read 2645802 spots for ERR11006604.sra Written 2645802 spots for ERR11006604.sra Written 2645802 spots for ERR11006604.sra Read 2645802 spots for ERR11006604.sra Written 2645802 spots for ERR11006604.sra Read 2645802 spots for ERR11006604.sra Written 2645802 spots for ERR11006604.sra Read 2645802 spots for ERR11006604.sra Written 2645802 spots for ERR11006604.sra Read 2645802 spots for ERR11006604.sra Written 2645802 spots for ERR11006604.sra Read 2645802 spots for ERR11006604.sra Written 2645802 spots for ERR11006604.sra Read 2645802 spots for ERR11006604.sra Written 2645802 spots for ERR11006604.sra Read 2645802 spots for ERR11006604.sra Written 2645802 spots for ERR11006604.sra Read 2645802 spots for ERR11006604.sra Written 2645802 spots for ERR11006604.sra Read 2645802 spots for ERR11006604.sra Written 2645802 spots for ERR11006604.sra Read 2645804 spots for ERR11006604.sra Written 2645804 spots for ERR11006604.sra Read 2645802 spots for ERR11006604.sra Written 2645802 spots for ERR11006604.sra SRR ids: ['ERR11006604.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_uf_gb7g4 ERR11006604.sra spots: 52916042 blocks: [[1, 2645802], [2645803, 5291604], [5291605, 7937406], [7937407, 10583208], [10583209, 13229010], [13229011, 15874812], [15874813, 18520614], [18520615, 21166416], [21166417, 23812218], [23812219, 26458020], [26458021, 29103822], [29103823, 31749624], [31749625, 34395426], [34395427, 37041228], [37041229, 39687030], [39687031, 42332832], [42332833, 44978634], [44978635, 47624436], [47624437, 50270238], [50270239, 52916042]] ERR11006604 file size 19443757 ERR11006604 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006604 ERR11006604_1.fastq ERR11006604_2.fastq Input file: ERR11006604_1.fastq Paired file: ERR11006604_2.fastq trimmed: ERR11006604-trimmed-pair1.fastq, ERR11006604-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 19:32:05 2024 >> started Fri Dec 6 19:33:08 2024 >> done (63.340s) 52916042 read pairs processed; of these: 556 ( 0.00%) short read pairs filtered out after trimming by size control 856 ( 0.00%) empty read pairs filtered out after trimming by size control 52914630 (100.00%) read pairs available; of these: 124556 ( 0.24%) trimmed read pairs available after processing 52790074 (99.76%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 23 0.00% 19 20 0.00% 20 16 0.00% 21 33 0.00% 22 67 0.00% 23 16 0.00% 24 27 0.00% 25 25 0.00% 26 21 0.00% 27 14 0.00% 28 29 0.00% 29 22 0.00% 30 28 0.00% 31 53 0.00% 32 19 0.00% 33 25 0.00% 34 28 0.00% 35 41 0.00% 36 28 0.00% 37 23 0.00% 38 33 0.00% 39 30 0.00% 40 41 0.00% 41 46 0.00% 42 32 0.00% 43 26 0.00% 44 37 0.00% 45 39 0.00% 46 43 0.00% 47 37 0.00% 48 38 0.00% 49 36 0.00% 50 45 0.00% 51 42 0.00% 52 46 0.00% 53 53 0.00% 54 50 0.00% 55 70 0.00% 56 54 0.00% 57 63 0.00% 58 60 0.00% 59 61 0.00% 60 65 0.00% 61 66 0.00% 62 85 0.00% 63 83 0.00% 64 94 0.00% 65 102 0.00% 66 86 0.00% 67 118 0.00% 68 110 0.00% 69 107 0.00% 70 118 0.00% 71 138 0.00% 72 132 0.00% 73 131 0.00% 74 128 0.00% 75 150 0.00% 76 174 0.00% 77 211 0.00% 78 179 0.00% 79 227 0.00% 80 223 0.00% 81 224 0.00% 82 238 0.00% 83 241 0.00% 84 248 0.00% 85 267 0.00% 86 302 0.00% 87 327 0.00% 88 347 0.00% 89 369 0.00% 90 395 0.00% 91 380 0.00% 92 396 0.00% 93 419 0.00% 94 397 0.00% 95 454 0.00% 96 504 0.00% 97 589 0.00% 98 627 0.00% 99 637 0.00% 100 646 0.00% 101 689 0.00% 102 679 0.00% 103 754 0.00% 104 767 0.00% 105 791 0.00% 106 893 0.00% 107 839 0.00% 108 917 0.00% 109 974 0.00% 110 1007 0.00% 111 1025 0.00% 112 1220 0.00% 113 1167 0.00% 114 1218 0.00% 115 1265 0.00% 116 1364 0.00% 117 1471 0.00% 118 1456 0.00% 119 1574 0.00% 120 1715 0.00% 121 1732 0.00% 122 1932 0.00% 123 2008 0.00% 124 2000 0.00% 125 2397 0.00% 126 1967 0.00% 127 2038 0.00% 128 2176 0.00% 129 2473 0.00% 130 2576 0.00% 131 2591 0.00% 132 2780 0.01% 133 2692 0.01% 134 2884 0.01% 135 2630 0.00% 136 2837 0.01% 137 2861 0.01% 138 2980 0.01% 139 3206 0.01% 140 3138 0.01% 141 3444 0.01% 142 3677 0.01% 143 4064 0.01% 144 3962 0.01% 145 4389 0.01% 146 5716 0.01% 147 4938 0.01% 148 4950 0.01% 149 5459 0.01% 150 52790074 99.76% 52914630 reads passed initial QC criterion=sequence-density sequence-density=0.32 sequence-density-rank=1 fanout-score=0.00 fanout-score-rank=34 prefix-density=0.00 prefix-fanout=1.0 sequence=TGCGGGAACTTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=90.72 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=2.0 sequence=AAAAACAGTAGAAGTAGAACAGGTATAAATAAGAAAATCTTAGTTAAGAGGGTTCATGTAAAGAACAGGTTCTAAATCACGATCGATTCCCTTTTCAAAACCTGCTGCAGCAGCTCGGGCTCTTCCTGCATGCCACAAATGGCCCACAAAAAAGAAGAATCCTAGAACAAAATGAGAAGTCGATAACCAACTTCTAGGAGAGACATAATTAACTGCATTGATCTCGGTAGCTACGCCACCCACGGAATTTAAAGAGCCTAAAGGAGCATGGGTCATATATTCCGCTGAACGTCGTTCTTGCCAAGGTTGTATGTCTTTTTTCAACCTACTCAAGTCCAAACCGTTGGGCCCCCTTAGAGGTTCTAACCATGGAGCACGGAGGTCCCAAAAACGCATAGTTTCCCCTCCAAAGATAACCTCTCCCGTTGGGGAACGCATTAGATATTTACCTAAACCTGTGGGTCCTTGAGCAGATCCCACATTAGCTCCAAGACGCTGGTCTCTAACTAGA criterion=sequence-density sequence-density=0.27 sequence-density-rank=1 fanout-score=42.90 fanout-score-rank=5 prefix-density=11.44 prefix-fanout=1.0 sequence=GCACTGAAAATAGTCTTTACA criterion=fanout-score sequence-density=0.01 sequence-density-rank=44 fanout-score=311.10 fanout-score-rank=1 prefix-density=0.69 prefix-fanout=2.3 sequence=TACTTCCATTAGTCCCCGTGTTCTTCGAATGGATCTCTTAATTGTTGAGAGGGTTGCCCAAACGCGGTATATAAGGCATACCCAGTAAAGCTTACAAGTAAACCAGATATGGAGATGGCGACTAAAGTTGCTGTTTCCATTTTTATAGAATTTAAAGATTACAATGGATCTACAAAAAGATCGT ERR11006604 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 19:33:44 Started mapping on | Dec 06 19:33:44 Finished on | Dec 06 19:38:39 Mapping speed, Million of reads per hour | 645.74 Number of input reads | 52914630 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 39795663 Uniquely mapped reads % | 75.21% Average mapped length | 298.62 Number of splices: Total | 11175882 Number of splices: Annotated (sjdb) | 10332753 Number of splices: GT/AG | 10851817 Number of splices: GC/AG | 133479 Number of splices: AT/AC | 17947 Number of splices: Non-canonical | 172639 Mismatch rate per base, % | 0.31% Deletion rate per base | 0.01% Deletion average length | 1.76 Insertion rate per base | 0.01% Insertion average length | 2.01 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 11624414 % of reads mapped to multiple loci | 21.97% Number of reads mapped to too many loci | 5564 % of reads mapped to too many loci | 0.01% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.71% % of reads unmapped: other | 0.10% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1494553 1494553 1494553 N_multimapping 11624414 11624414 11624414 N_noFeature 9986376 36538077 12076815 N_ambiguous 1985303 63945 833082 UnstrandedReadsAssigned:27823984 PositiveStrandReadsAssigned:3193641 NegativeStrandReadsAssigned:26885766 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006604 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006604-trimmed-pair1.fastq ERR11006604-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 52,914,630 reads, 30,796,977 reads pseudoaligned [quant] estimated average fragment length: 318.352 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,093 rounds 52973 ERR11006604.ke.tsv 35125 ERR11006604.se.tsv 88098 total ==> ERR11006604.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 619.121 0 0 PNS24247 1044 726.648 16.1782 0.59842 PNS24249 1928 1610.65 50.1693 0.837218 PNS24246 1044 726.648 16.1782 0.59842 PNS24248 1044 726.648 16.1782 0.59842 PNS24244 1471 1153.65 53.2962 1.24172 PNS24243 293 40.5939 0 0 KQK14069 1603 1285.65 2915.87 60.9602 KQK14071 474 162.564 12.771 2.11155 ==> ERR11006604.se.tsv <== BRADI_1g14170v3 3054 BRADI_1g53295v3 14 BRADI_1g59795v3 157 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 107 BRADI_1g74790v3 114 BRADI_1g09890v3 0 BRADI_1g77505v3 217 BRADI_1g48960v3 2 ERR11006604 completed mapping pipeline successfully