Starting /dee2/code/volunteer_pipeline.sh ERR11006605 current disk space = 1549841100800 free memory = 1325549032 ERR11006605 SRAfilesize 3f562e0fd88e04f5e4d504111e7239f5 ERR11006605.sra ERR11006605.sra file validated ERR11006605 is paired end ERR11006605 is conventional basespace ERR11006605 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006605_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.3265 37.0 37.0 37.0 37.0 37.0 2 36.0565 37.0 37.0 37.0 37.0 37.0 3 36.241 37.0 37.0 37.0 37.0 37.0 4 36.427 37.0 37.0 37.0 37.0 37.0 5 36.32 37.0 37.0 37.0 37.0 37.0 6 36.368 37.0 37.0 37.0 37.0 37.0 7 36.2495 37.0 37.0 37.0 37.0 37.0 8 36.4805 37.0 37.0 37.0 37.0 37.0 9 36.3865 37.0 37.0 37.0 37.0 37.0 10-14 36.4182 37.0 37.0 37.0 37.0 37.0 15-19 36.4053 37.0 37.0 37.0 37.0 37.0 20-24 36.320299999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.2755 37.0 37.0 37.0 37.0 37.0 30-34 36.206900000000005 37.0 37.0 37.0 37.0 37.0 35-39 36.2013 37.0 37.0 37.0 37.0 37.0 40-44 36.1894 37.0 37.0 37.0 37.0 37.0 45-49 36.20139999999999 37.0 37.0 37.0 37.0 37.0 50-54 36.1307 37.0 37.0 37.0 37.0 37.0 55-59 36.1166 37.0 37.0 37.0 37.0 37.0 60-64 36.1092 37.0 37.0 37.0 37.0 37.0 65-69 36.118700000000004 37.0 37.0 37.0 37.0 37.0 70-74 36.095299999999995 37.0 37.0 37.0 37.0 37.0 75-79 35.997 37.0 37.0 37.0 37.0 37.0 80-84 35.9513 37.0 37.0 37.0 37.0 37.0 85-89 35.9221 37.0 37.0 37.0 37.0 37.0 90-94 35.793899999999994 37.0 37.0 37.0 37.0 37.0 95-99 35.894999999999996 37.0 37.0 37.0 37.0 37.0 100-104 35.7825 37.0 37.0 37.0 37.0 37.0 105-109 35.7154 37.0 37.0 37.0 37.0 37.0 110-114 35.612 37.0 37.0 37.0 37.0 37.0 115-119 35.6028 37.0 37.0 37.0 37.0 37.0 120-124 35.6499 37.0 37.0 37.0 37.0 37.0 125-129 35.498000000000005 37.0 37.0 37.0 37.0 37.0 130-134 35.3481 37.0 37.0 37.0 34.6 37.0 135-139 35.350100000000005 37.0 37.0 37.0 37.0 37.0 140-144 35.1862 37.0 37.0 37.0 29.8 37.0 145-149 35.3292 37.0 37.0 37.0 32.2 37.0 150 35.259 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 0.0 23 2.0 24 3.0 25 14.0 26 10.0 27 14.0 28 25.0 29 37.0 30 51.0 31 73.0 32 80.0 33 124.0 34 167.0 35 291.0 36 2809.0 37 299.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.824999999999996 13.25 15.275 33.650000000000006 2 28.849999999999998 11.200000000000001 25.575 34.375 3 22.675 13.425 27.55 36.35 4 26.900000000000002 16.25 26.125 30.725 5 28.675 19.325 24.725 27.275 6 24.15 29.325000000000003 20.275000000000002 26.25 7 15.925 32.375 34.849999999999994 16.85 8 16.625 29.625 34.875 18.875 9 16.150000000000002 28.849999999999998 35.825 19.175 10-14 19.555 32.735 26.82 20.89 15-19 19.695 34.005 25.34 20.96 20-24 19.08 29.695 28.244999999999997 22.98 25-29 21.055 32.324999999999996 26.040000000000003 20.580000000000002 30-34 22.31 31.655 25.765 20.27 35-39 21.72 32.61 25.474999999999998 20.195 40-44 19.74 31.47 25.905 22.884999999999998 45-49 19.245 30.91 27.450000000000003 22.395 50-54 20.135 33.885 25.255 20.724999999999998 55-59 21.36 30.570000000000004 24.005000000000003 24.065 60-64 20.635 32.029999999999994 25.05 22.285 65-69 20.880000000000003 30.86 25.19 23.07 70-74 21.81 31.72 22.295 24.175 75-79 21.815 30.775000000000002 25.35 22.06 80-84 22.535 30.055 24.625 22.785 85-89 23.225 30.335 24.795 21.645 90-94 21.560000000000002 30.625000000000004 26.505000000000003 21.310000000000002 95-99 23.23 30.314999999999998 23.875 22.58 100-104 20.86 31.405 25.085 22.650000000000002 105-109 21.0 29.904999999999998 26.63 22.465 110-114 22.27 29.7 25.5 22.53 115-119 19.185 32.07 25.56 23.185 120-124 19.455 30.0 25.8 24.745 125-129 20.365 32.635 23.34 23.66 130-134 21.87 31.230000000000004 25.045 21.855 135-139 22.74 30.495 23.189999999999998 23.575 140-144 23.39 30.375000000000004 26.009999999999998 20.225 145-149 22.045 31.759999999999998 25.169999999999998 21.025 150 22.925 27.750000000000004 28.15 21.175 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 1.0 21 3.0 22 2.5 23 1.5 24 5.0 25 7.5 26 9.5 27 13.5 28 17.0 29 21.5 30 28.0 31 31.0 32 31.5 33 38.0 34 48.0 35 57.0 36 82.0 37 184.5 38 312.5 39 284.5 40 252.5 41 309.5 42 296.0 43 265.5 44 233.0 45 212.5 46 191.5 47 155.0 48 132.0 49 85.5 50 53.5 51 49.5 52 38.0 53 34.0 54 35.0 55 35.5 56 29.5 57 17.0 58 21.5 59 23.5 60 21.5 61 22.5 62 16.5 63 14.0 64 29.5 65 45.5 66 34.0 67 14.0 68 16.0 69 22.0 70 17.0 71 13.5 72 14.5 73 13.5 74 12.0 75 10.0 76 6.5 77 5.5 78 4.5 79 4.0 80 4.0 81 3.0 82 2.0 83 1.5 84 1.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.724999999999994 #Duplication Level Percentage of deduplicated Percentage of total 1 78.35791151853329 49.15 2 13.750498206456754 17.25 3 3.1088082901554404 5.8500000000000005 4 1.434834595456357 3.5999999999999996 5 0.876843363889996 2.75 6 0.3985651654045437 1.5 7 0.438421681944998 1.925 8 0.3188521323236349 1.6 9 0.23913909924272617 1.35 >10 1.0362694300518136 11.5 >50 0.0 0.0 >100 0.03985651654045436 3.5249999999999995 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 141 3.5249999999999995 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 42 1.05 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 34 0.8500000000000001 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 33 0.8250000000000001 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 31 0.775 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 29 0.7250000000000001 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 21 0.525 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 20 0.5 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 19 0.475 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 19 0.475 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 16 0.4 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 16 0.4 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 15 0.375 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 15 0.375 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 14 0.35000000000000003 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 14 0.35000000000000003 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 13 0.325 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 12 0.3 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 12 0.3 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 12 0.3 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 11 0.27499999999999997 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 11 0.27499999999999997 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 11 0.27499999999999997 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 10 0.25 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 10 0.25 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 10 0.25 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 10 0.25 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 9 0.22499999999999998 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 9 0.22499999999999998 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 9 0.22499999999999998 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 9 0.22499999999999998 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 9 0.22499999999999998 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 9 0.22499999999999998 No Hit CCGCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGAT 8 0.2 No Hit CAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGG 8 0.2 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 8 0.2 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 8 0.2 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 8 0.2 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 8 0.2 No Hit CACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCA 8 0.2 No Hit TCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTT 8 0.2 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 7 0.17500000000000002 No Hit CCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTT 7 0.17500000000000002 No Hit ATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCT 7 0.17500000000000002 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 7 0.17500000000000002 No Hit TAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCA 7 0.17500000000000002 No Hit GCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAGCTGCAA 7 0.17500000000000002 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 7 0.17500000000000002 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 7 0.17500000000000002 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 7 0.17500000000000002 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 7 0.17500000000000002 No Hit GGGGAATTCGTAGATCCTCCAGACGTAGAGCACGTAGGGCTTTGAAACCA 7 0.17500000000000002 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 6 0.15 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 6 0.15 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 6 0.15 No Hit GCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTC 6 0.15 No Hit TCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGT 6 0.15 No Hit GTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCA 6 0.15 No Hit ATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAG 6 0.15 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 6 0.15 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 6 0.15 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 6 0.15 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 5 0.125 No Hit TCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCG 5 0.125 No Hit TGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAAC 5 0.125 No Hit GGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAA 5 0.125 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 5 0.125 No Hit CGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTT 5 0.125 No Hit GCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTAAG 5 0.125 No Hit GCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCA 5 0.125 No Hit ACCCTCTTCAAATAGATCTAATGGATAAGCTACATAACAGATCCATTGAC 5 0.125 No Hit AGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAG 5 0.125 No Hit TCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGT 5 0.125 No Hit CCATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGT 5 0.125 No Hit AGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCAT 5 0.125 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 5 0.125 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 5 0.125 No Hit TAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCA 5 0.125 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 5 0.125 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 5 0.125 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 5 0.125 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 5 0.125 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 5 0.125 No Hit GTCGAGAGATAGCTCTCCATACACTGATAAGGGGTGTATGGATTCTCGAG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.025 0.0 0.0 0.0 0.0 110-111 0.025 0.0 0.0 0.0 0.0 112-113 0.025 0.0 0.0 0.0 0.0 114-115 0.025 0.0 0.0 0.0 0.0 116-117 0.025 0.0 0.0 0.0 0.0 118-119 0.025 0.0 0.0 0.0 0.0 120-121 0.05 0.0 0.0 0.0 0.0 122-123 0.0625 0.0 0.0 0.0 0.0 124-125 0.075 0.0 0.0 0.0 0.0 126-127 0.1 0.0 0.0 0.0 0.0 128-129 0.1375 0.0 0.0 0.0 0.0 130-131 0.2 0.0 0.0 0.0 0.0 132-133 0.2625 0.0 0.0 0.0 0.0 134-135 0.2875 0.0 0.0 0.0 0.0 136-137 0.375 0.0 0.0 0.0 0.0 138 0.4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACCTTA 20 0.006139246 28.8 40-44 TTCTTAT 20 0.006139246 28.8 20-24 ATATGTT 20 0.006139246 28.8 25-29 GGAAAAA 20 0.006139246 28.8 35-39 >>END_MODULE ERR11006605 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006605_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.617 37.0 37.0 37.0 37.0 37.0 2 35.8235 37.0 37.0 37.0 37.0 37.0 3 36.0215 37.0 37.0 37.0 37.0 37.0 4 35.9195 37.0 37.0 37.0 37.0 37.0 5 36.0885 37.0 37.0 37.0 37.0 37.0 6 36.081 37.0 37.0 37.0 37.0 37.0 7 35.911 37.0 37.0 37.0 37.0 37.0 8 36.162 37.0 37.0 37.0 37.0 37.0 9 36.0535 37.0 37.0 37.0 37.0 37.0 10-14 36.131600000000006 37.0 37.0 37.0 37.0 37.0 15-19 36.0524 37.0 37.0 37.0 37.0 37.0 20-24 36.027 37.0 37.0 37.0 37.0 37.0 25-29 36.043099999999995 37.0 37.0 37.0 37.0 37.0 30-34 35.9636 37.0 37.0 37.0 37.0 37.0 35-39 35.9533 37.0 37.0 37.0 37.0 37.0 40-44 35.9077 37.0 37.0 37.0 37.0 37.0 45-49 35.8637 37.0 37.0 37.0 37.0 37.0 50-54 35.78920000000001 37.0 37.0 37.0 37.0 37.0 55-59 35.7547 37.0 37.0 37.0 37.0 37.0 60-64 35.6462 37.0 37.0 37.0 37.0 37.0 65-69 35.6403 37.0 37.0 37.0 37.0 37.0 70-74 35.6051 37.0 37.0 37.0 37.0 37.0 75-79 35.6168 37.0 37.0 37.0 37.0 37.0 80-84 35.5641 37.0 37.0 37.0 37.0 37.0 85-89 35.4803 37.0 37.0 37.0 37.0 37.0 90-94 35.444399999999995 37.0 37.0 37.0 37.0 37.0 95-99 35.1807 37.0 37.0 37.0 27.4 37.0 100-104 35.2783 37.0 37.0 37.0 29.8 37.0 105-109 35.1882 37.0 37.0 37.0 27.4 37.0 110-114 35.0393 37.0 37.0 37.0 25.0 37.0 115-119 34.9824 37.0 37.0 37.0 25.0 37.0 120-124 34.915499999999994 37.0 37.0 37.0 25.0 37.0 125-129 35.0184 37.0 37.0 37.0 25.0 37.0 130-134 34.8264 37.0 37.0 37.0 25.0 37.0 135-139 34.708 37.0 37.0 37.0 25.0 37.0 140-144 34.69520000000001 37.0 37.0 37.0 25.0 37.0 145-149 34.70479999999999 37.0 37.0 37.0 25.0 37.0 150 34.39 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0 20 1.0 21 1.0 22 6.0 23 8.0 24 7.0 25 9.0 26 27.0 27 16.0 28 34.0 29 43.0 30 55.0 31 63.0 32 96.0 33 140.0 34 267.0 35 757.0 36 2389.0 37 79.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.94870492635856 25.36820721178263 18.131030980192993 27.55205688166582 2 24.825 24.55 33.4 17.224999999999998 3 20.4 27.3 33.125 19.175 4 21.65 28.95 29.349999999999998 20.05 5 24.15 27.725 29.95 18.175 6 21.025 32.025 28.499999999999996 18.45 7 19.15 21.7 40.975 18.175 8 21.099999999999998 23.200000000000003 32.125 23.575 9 22.775000000000002 20.200000000000003 35.15 21.875 10-14 23.07 26.805 29.330000000000002 20.794999999999998 15-19 23.515 24.83 31.39 20.265 20-24 23.35 24.39 31.34 20.919999999999998 25-29 23.29 25.365 30.855 20.49 30-34 23.244999999999997 25.1 31.165 20.49 35-39 23.580000000000002 25.06 30.380000000000003 20.979999999999997 40-44 22.36 25.669999999999998 30.380000000000003 21.59 45-49 22.39 27.169999999999998 30.049999999999997 20.39 50-54 22.425 26.005 29.799999999999997 21.77 55-59 22.38 25.555 30.72 21.345 60-64 22.58 25.81 30.259999999999998 21.349999999999998 65-69 22.830000000000002 25.674999999999997 29.695 21.8 70-74 23.255 26.495 30.0 20.25 75-79 22.869999999999997 25.535000000000004 30.615 20.979999999999997 80-84 24.310000000000002 25.729999999999997 30.175 19.785 85-89 23.45 25.840000000000003 29.42 21.29 90-94 23.26 24.98 30.225 21.535 95-99 23.365 24.955 30.205 21.475 100-104 23.625 24.404999999999998 31.395 20.575 105-109 23.605 24.59 30.835 20.97 110-114 23.150000000000002 24.67 30.825000000000003 21.355 115-119 22.755 25.865 30.45 20.93 120-124 23.43 25.28 29.57 21.72 125-129 22.08 25.955000000000002 30.620000000000005 21.345 130-134 22.485 26.66 30.11 20.745 135-139 23.025000000000002 26.1 30.349999999999998 20.525 140-144 22.720000000000002 25.53 31.035 20.715 145-149 22.025 25.264999999999997 31.285 21.425 150 21.925 23.825 32.4 21.85 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 1.0 19 2.0 20 2.0 21 1.5 22 3.5 23 6.0 24 6.0 25 9.0 26 14.0 27 17.5 28 25.0 29 31.0 30 32.0 31 34.0 32 43.5 33 46.5 34 52.5 35 88.0 36 133.0 37 199.5 38 233.0 39 221.0 40 240.5 41 269.0 42 256.5 43 237.0 44 238.0 45 234.5 46 206.0 47 143.0 48 108.5 49 94.0 50 73.5 51 61.0 52 44.0 53 36.5 54 36.0 55 36.0 56 29.5 57 15.5 58 19.5 59 32.0 60 33.0 61 24.0 62 21.0 63 39.0 64 40.5 65 28.5 66 24.0 67 17.0 68 16.5 69 19.5 70 17.0 71 12.5 72 9.0 73 10.0 74 15.5 75 15.5 76 13.0 77 9.0 78 6.5 79 5.0 80 2.0 81 1.0 82 0.5 83 1.5 84 2.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.55 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.025 #Duplication Level Percentage of deduplicated Percentage of total 1 78.53730246233003 53.425 2 12.458654906284455 16.950000000000003 3 4.336640940830577 8.85 4 1.6905549430356486 4.6 5 0.8085262771040059 2.75 6 0.5880191106210952 2.4 7 0.47776552737963984 2.275 8 0.33076074972436603 1.7999999999999998 9 0.18375597206909225 1.125 >10 0.5512679162072767 4.5249999999999995 >50 0.03675119441381845 1.3 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 52 1.3 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 17 0.42500000000000004 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 17 0.42500000000000004 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 16 0.4 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 15 0.375 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 12 0.3 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 12 0.3 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 11 0.27499999999999997 No Hit GGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACA 11 0.27499999999999997 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 10 0.25 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 10 0.25 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 10 0.25 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 10 0.25 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 10 0.25 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 10 0.25 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 10 0.25 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 9 0.22499999999999998 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 9 0.22499999999999998 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 9 0.22499999999999998 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 9 0.22499999999999998 No Hit AACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGG 9 0.22499999999999998 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 8 0.2 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 8 0.2 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 8 0.2 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 8 0.2 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 8 0.2 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 8 0.2 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 8 0.2 No Hit GCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGC 8 0.2 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 8 0.2 No Hit GCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTTATG 7 0.17500000000000002 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 7 0.17500000000000002 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 7 0.17500000000000002 No Hit GTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTT 7 0.17500000000000002 No Hit GATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTC 7 0.17500000000000002 No Hit ATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGA 7 0.17500000000000002 No Hit GTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACT 7 0.17500000000000002 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 7 0.17500000000000002 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 7 0.17500000000000002 No Hit TTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGG 7 0.17500000000000002 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 7 0.17500000000000002 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 7 0.17500000000000002 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 7 0.17500000000000002 No Hit CCTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTAT 6 0.15 No Hit CTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGT 6 0.15 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 6 0.15 No Hit AATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTAT 6 0.15 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 6 0.15 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 6 0.15 No Hit CCTCTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGT 6 0.15 No Hit ATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGT 6 0.15 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 6 0.15 No Hit CTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGG 6 0.15 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 6 0.15 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 6 0.15 No Hit CAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTA 6 0.15 No Hit CGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAACTTCTG 6 0.15 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 6 0.15 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 6 0.15 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 5 0.125 No Hit AAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATC 5 0.125 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 5 0.125 No Hit ATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATG 5 0.125 No Hit ATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCT 5 0.125 No Hit GCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTC 5 0.125 No Hit GACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCC 5 0.125 No Hit TCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGG 5 0.125 No Hit CTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGC 5 0.125 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 5 0.125 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 5 0.125 No Hit AAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAA 5 0.125 No Hit AGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCT 5 0.125 No Hit CTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAG 5 0.125 No Hit ATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTAC 5 0.125 No Hit TGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATT 5 0.125 No Hit GTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACA 5 0.125 No Hit GTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAG 5 0.125 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 5 0.125 No Hit AATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTA 5 0.125 No Hit CTTGTTATTGTGAGAATTCTTAATTCAAGAGTTGTAAGGAGGGACTTATG 5 0.125 No Hit CTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.025 0.0 0.0 0.0 0.0 110-111 0.025 0.0 0.0 0.0 0.0 112-113 0.025 0.0 0.0 0.0 0.0 114-115 0.025 0.0 0.0 0.0 0.0 116-117 0.025 0.0 0.0 0.0 0.0 118-119 0.025 0.0 0.0 0.0 0.0 120-121 0.05 0.0 0.0 0.0 0.0 122-123 0.0625 0.0 0.0 0.0 0.0 124-125 0.075 0.0 0.0 0.0 0.0 126-127 0.125 0.0 0.0 0.0 0.0 128-129 0.16249999999999998 0.0 0.0 0.0 0.0 130-131 0.225 0.0 0.0 0.0 0.0 132-133 0.2875 0.0 0.0 0.0 0.0 134-135 0.3125 0.0 0.0 0.0 0.0 136-137 0.4 0.0 0.0 0.0 0.0 138 0.425 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 2592978 spots for ERR11006605.sra Written 2592978 spots for ERR11006605.sra Read 2592978 spots for ERR11006605.sra Written 2592978 spots for ERR11006605.sra Read 2592978 spots for ERR11006605.sra Written 2592978 spots for ERR11006605.sra Read 2592978 spots for ERR11006605.sra Written 2592978 spots for ERR11006605.sra Read 2592978 spots for ERR11006605.sra Read 2592986 spots for ERR11006605.sra Written 2592978 spots for ERR11006605.sra Written 2592986 spots for ERR11006605.sra Read 2592978 spots for ERR11006605.sra Written 2592978 spots for ERR11006605.sra Read 2592978 spots for ERR11006605.sra Written 2592978 spots for ERR11006605.sra Read 2592978 spots for ERR11006605.sra Written 2592978 spots for ERR11006605.sra Read 2592978 spots for ERR11006605.sra Written 2592978 spots for ERR11006605.sra Read 2592978 spots for ERR11006605.sra Written 2592978 spots for ERR11006605.sra Read 2592978 spots for ERR11006605.sra Written 2592978 spots for ERR11006605.sra Read 2592978 spots for ERR11006605.sra Written 2592978 spots for ERR11006605.sra Read 2592978 spots for ERR11006605.sra Written 2592978 spots for ERR11006605.sra Read 2592978 spots for ERR11006605.sra Written 2592978 spots for ERR11006605.sra Read 2592978 spots for ERR11006605.sra Written 2592978 spots for ERR11006605.sra Read 2592978 spots for ERR11006605.sra Written 2592978 spots for ERR11006605.sra Read 2592978 spots for ERR11006605.sra Written 2592978 spots for ERR11006605.sra Read 2592978 spots for ERR11006605.sra Written 2592978 spots for ERR11006605.sra Read 2592978 spots for ERR11006605.sra Written 2592978 spots for ERR11006605.sra SRR ids: ['ERR11006605.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_ya1qc0w_ ERR11006605.sra spots: 51859568 blocks: [[1, 2592978], [2592979, 5185956], [5185957, 7778934], [7778935, 10371912], [10371913, 12964890], [12964891, 15557868], [15557869, 18150846], [18150847, 20743824], [20743825, 23336802], [23336803, 25929780], [25929781, 28522758], [28522759, 31115736], [31115737, 33708714], [33708715, 36301692], [36301693, 38894670], [38894671, 41487648], [41487649, 44080626], [44080627, 46673604], [46673605, 49266582], [49266583, 51859568]] ERR11006605 file size 19054942 ERR11006605 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006605 ERR11006605_1.fastq ERR11006605_2.fastq Input file: ERR11006605_1.fastq Paired file: ERR11006605_2.fastq trimmed: ERR11006605-trimmed-pair1.fastq, ERR11006605-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 19:35:33 2024 >> started Fri Dec 6 19:36:44 2024 >> done (71.556s) 51859568 read pairs processed; of these: 161 ( 0.00%) short read pairs filtered out after trimming by size control 1361 ( 0.00%) empty read pairs filtered out after trimming by size control 51858046 (100.00%) read pairs available; of these: 502739 ( 0.97%) trimmed read pairs available after processing 51355307 (99.03%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 1 0.00% 20 7 0.00% 21 14 0.00% 22 18 0.00% 23 3 0.00% 24 6 0.00% 25 10 0.00% 26 7 0.00% 27 12 0.00% 28 12 0.00% 29 16 0.00% 30 11 0.00% 31 57 0.00% 32 7 0.00% 33 7 0.00% 34 10 0.00% 35 7 0.00% 36 9 0.00% 37 8 0.00% 38 10 0.00% 39 16 0.00% 40 19 0.00% 41 10 0.00% 42 22 0.00% 43 17 0.00% 44 29 0.00% 45 23 0.00% 46 14 0.00% 47 21 0.00% 48 38 0.00% 49 26 0.00% 50 27 0.00% 51 38 0.00% 52 33 0.00% 53 51 0.00% 54 42 0.00% 55 60 0.00% 56 53 0.00% 57 58 0.00% 58 68 0.00% 59 77 0.00% 60 69 0.00% 61 92 0.00% 62 130 0.00% 63 123 0.00% 64 139 0.00% 65 130 0.00% 66 167 0.00% 67 172 0.00% 68 185 0.00% 69 221 0.00% 70 216 0.00% 71 254 0.00% 72 291 0.00% 73 351 0.00% 74 328 0.00% 75 398 0.00% 76 405 0.00% 77 452 0.00% 78 526 0.00% 79 517 0.00% 80 540 0.00% 81 560 0.00% 82 648 0.00% 83 745 0.00% 84 755 0.00% 85 873 0.00% 86 983 0.00% 87 1062 0.00% 88 1080 0.00% 89 1132 0.00% 90 1246 0.00% 91 1264 0.00% 92 1389 0.00% 93 1453 0.00% 94 1512 0.00% 95 1714 0.00% 96 1803 0.00% 97 1942 0.00% 98 2008 0.00% 99 2213 0.00% 100 2262 0.00% 101 2398 0.00% 102 2472 0.00% 103 2654 0.01% 104 2835 0.01% 105 2915 0.01% 106 3175 0.01% 107 3272 0.01% 108 3548 0.01% 109 3765 0.01% 110 4140 0.01% 111 4209 0.01% 112 4564 0.01% 113 4464 0.01% 114 4673 0.01% 115 4937 0.01% 116 5211 0.01% 117 5457 0.01% 118 5593 0.01% 119 6157 0.01% 120 6720 0.01% 121 6850 0.01% 122 7521 0.01% 123 7749 0.01% 124 8093 0.02% 125 9035 0.02% 126 8462 0.02% 127 8380 0.02% 128 9035 0.02% 129 9958 0.02% 130 10485 0.02% 131 11388 0.02% 132 11634 0.02% 133 11056 0.02% 134 11887 0.02% 135 11774 0.02% 136 12417 0.02% 137 12746 0.02% 138 13371 0.03% 139 14307 0.03% 140 14484 0.03% 141 15677 0.03% 142 16538 0.03% 143 17471 0.03% 144 17920 0.03% 145 18907 0.04% 146 22381 0.04% 147 21209 0.04% 148 22451 0.04% 149 23064 0.04% 150 51355307 99.03% 51858046 reads passed initial QC criterion=sequence-density sequence-density=0.28 sequence-density-rank=1 fanout-score=0.00 fanout-score-rank=35 prefix-density=0.00 prefix-fanout=1.0 sequence=TGCGGGAACTTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=37 fanout-score=45.67 fanout-score-rank=1 prefix-density=0.10 prefix-fanout=2.4 sequence=TTTCTCTTTTTATTTTGTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTTATCTATTAATCGATAGTATCTACCGGCGCGAACTCGAATTTGATCGCCTTCCATACTTCACAAGCTGCGGCTAGTTCAGGACTCCATTTGCAAGCTGCTCGGATAATTTCATTACCTTCACGAGCAAGATCGCGCCCTTCGTTACGAGCTTGTACACAGGCTTCTAAAGCCACTCGATTAGCTGCTGCACCAGGTGCATTTCCCCAAGGATGTCCTAAAGTTCCTCCACCAAATTGTAATACAGAATCATCCCCAAAGATTTCGGTCAGAGCTGGCATATGCCAAACATGAATACCACCTGAAGCTACTGGTATAACACCTGGCATGGATACCCAGTCCTGAGTGAAAAAGATACCGCGAGCACGATCTTTTTCAATAAAATCGTCGCGCAATAAATCAACAAAACCTAAAGTGATTTCGCGTTCCCCTTCTAACTTACCTACTACTGTACCGGCGTG criterion=sequence-density sequence-density=0.23 sequence-density-rank=1 fanout-score=4.07 fanout-score-rank=27 prefix-density=0.46 prefix-fanout=2.1 sequence=GGGGAAGAGGATCAAGTTGGCCCTTGCGAATAACTTGATGCACTATCTCCCTTCAACCCTTTGAGCGAAATGTAGCAAAAGGAAGCAAAATCCATGGACCGACCCCATTGTCTCCACCCCGTAGGAACTACGAGATCACCCCAAGGACGCCTTCGGCGTCCAGGGGTCACGGACCGACCATAGACCCTGTTCAATAAGTGGAACACATTAGCCGTCCGCTCTCCGGTTGGGCAGTAAGGGTCGGAGAAGGGCAATCACTCGTTCTTAAAACCAGCATTCTTAAGTTTAAGATCAAAGAGTCGGGCGGAAAAAGGGGAGAGCTCCCCGTTCCTGGTTCTCCTGTAGCTGGATTCCCCGGAACCACAAGAATCCTTAGAATGGGATTCCAACTCAGCACCTTTTGTTTTGAGATTTTGAGAAGAGTTGCTCTTTGGAGAGCACAGTACGATGAAAGTTGTAAGCTGTGTTCGGGGGGGAGTTATTGTCTATCGTTGGCCTCTATGGTAGAACC criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=100.62 fanout-score-rank=1 prefix-density=0.22 prefix-fanout=4.2 sequence=AAAAAAAGATAAGAAGCAAAGTTTCCCCCTTGAGTTTGAGTGAAAAAACGAAAATGCGAGAAAAATTGCAATCCCTACCACGTAATAGTGCACCTACACGCCTTCATCGACGTTGTTTTTTGACTGGAAGACCTAGAGCTAACTATCGAGATTTTGGGCTATCCGGGCACGTACTTCGAGAAATGGTTTATGAATGTTTGTT ERR11006605 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 19:37:37 Started mapping on | Dec 06 19:37:37 Finished on | Dec 06 19:44:04 Mapping speed, Million of reads per hour | 482.40 Number of input reads | 51858046 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 36280944 Uniquely mapped reads % | 69.96% Average mapped length | 298.31 Number of splices: Total | 10509872 Number of splices: Annotated (sjdb) | 9754569 Number of splices: GT/AG | 10184287 Number of splices: GC/AG | 127375 Number of splices: AT/AC | 27492 Number of splices: Non-canonical | 170718 Mismatch rate per base, % | 0.32% Deletion rate per base | 0.01% Deletion average length | 1.70 Insertion rate per base | 0.01% Insertion average length | 2.05 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 13953436 % of reads mapped to multiple loci | 26.91% Number of reads mapped to too many loci | 6476 % of reads mapped to too many loci | 0.01% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.84% % of reads unmapped: other | 0.28% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1623666 1623666 1623666 N_multimapping 13953436 13953436 13953436 N_noFeature 10028354 33498939 11827903 N_ambiguous 1777100 45263 816077 UnstrandedReadsAssigned:24475490 PositiveStrandReadsAssigned:2736742 NegativeStrandReadsAssigned:23636964 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006605 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006605-trimmed-pair1.fastq ERR11006605-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 51,858,046 reads, 29,652,996 reads pseudoaligned [quant] estimated average fragment length: 268.272 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,071 rounds 52973 ERR11006605.ke.tsv 35125 ERR11006605.se.tsv 88098 total ==> ERR11006605.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 669.032 0 0 PNS24247 1044 776.728 13.755 0.584033 PNS24249 1928 1660.73 78.6164 1.5612 PNS24246 1044 776.728 13.755 0.584033 PNS24248 1044 776.728 13.755 0.584033 PNS24244 1471 1203.73 69.1185 1.8937 PNS24243 293 53.7974 0 0 KQK14069 1603 1335.73 1970.08 48.6418 KQK14071 474 208.933 38.1541 6.02252 ==> ERR11006605.se.tsv <== BRADI_1g14170v3 2166 BRADI_1g53295v3 42 BRADI_1g59795v3 169 BRADI_1g07683v3 0 BRADI_1g00485v3 1 BRADI_1g20270v3 100 BRADI_1g74790v3 165 BRADI_1g09890v3 0 BRADI_1g77505v3 148 BRADI_1g48960v3 0 ERR11006605 completed mapping pipeline successfully