Starting /dee2/code/volunteer_pipeline.sh ERR11006606 current disk space = 1549718167552 free memory = 1596454508 ERR11006606 SRAfilesize 8bb7a6e953cc6f04076953e148b9731f ERR11006606.sra ERR11006606.sra file validated ERR11006606 is paired end ERR11006606 is conventional basespace ERR11006606 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006606_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1635 37.0 37.0 37.0 37.0 37.0 2 36.239 37.0 37.0 37.0 37.0 37.0 3 36.178 37.0 37.0 37.0 37.0 37.0 4 36.3235 37.0 37.0 37.0 37.0 37.0 5 36.351 37.0 37.0 37.0 37.0 37.0 6 36.388 37.0 37.0 37.0 37.0 37.0 7 36.2465 37.0 37.0 37.0 37.0 37.0 8 36.367 37.0 37.0 37.0 37.0 37.0 9 36.354 37.0 37.0 37.0 37.0 37.0 10-14 36.401500000000006 37.0 37.0 37.0 37.0 37.0 15-19 36.3649 37.0 37.0 37.0 37.0 37.0 20-24 36.312 37.0 37.0 37.0 37.0 37.0 25-29 36.280699999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.268699999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.200399999999995 37.0 37.0 37.0 37.0 37.0 40-44 36.1907 37.0 37.0 37.0 37.0 37.0 45-49 36.1639 37.0 37.0 37.0 37.0 37.0 50-54 36.146100000000004 37.0 37.0 37.0 37.0 37.0 55-59 36.0684 37.0 37.0 37.0 37.0 37.0 60-64 36.0667 37.0 37.0 37.0 37.0 37.0 65-69 36.081399999999995 37.0 37.0 37.0 37.0 37.0 70-74 35.990899999999996 37.0 37.0 37.0 37.0 37.0 75-79 35.956500000000005 37.0 37.0 37.0 37.0 37.0 80-84 35.966 37.0 37.0 37.0 37.0 37.0 85-89 35.912400000000005 37.0 37.0 37.0 37.0 37.0 90-94 35.833200000000005 37.0 37.0 37.0 37.0 37.0 95-99 35.8872 37.0 37.0 37.0 37.0 37.0 100-104 35.8249 37.0 37.0 37.0 37.0 37.0 105-109 35.7675 37.0 37.0 37.0 37.0 37.0 110-114 35.672000000000004 37.0 37.0 37.0 37.0 37.0 115-119 35.693999999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.656499999999994 37.0 37.0 37.0 37.0 37.0 125-129 35.60360000000001 37.0 37.0 37.0 37.0 37.0 130-134 35.3779 37.0 37.0 37.0 34.6 37.0 135-139 35.3969 37.0 37.0 37.0 37.0 37.0 140-144 35.1986 37.0 37.0 37.0 27.4 37.0 145-149 35.2831 37.0 37.0 37.0 32.2 37.0 150 35.3295 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 2.0 24 4.0 25 6.0 26 10.0 27 19.0 28 26.0 29 44.0 30 48.0 31 59.0 32 90.0 33 122.0 34 158.0 35 308.0 36 2856.0 37 248.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.325 12.35 16.0 34.325 2 28.775000000000002 10.925 27.250000000000004 33.050000000000004 3 21.8 11.075 28.975 38.15 4 25.85 16.05 26.75 31.35 5 28.9 19.925 24.4 26.775 6 25.25 29.225 21.224999999999998 24.3 7 16.7 30.875000000000004 34.625 17.8 8 17.5 28.95 34.125 19.425 9 15.675 28.475 37.875 17.974999999999998 10-14 19.505 31.869999999999997 27.3 21.325 15-19 19.885 34.62 24.98 20.515 20-24 18.64 29.115000000000002 29.304999999999996 22.939999999999998 25-29 21.355 32.16 25.924999999999997 20.560000000000002 30-34 23.305 30.505 25.595000000000002 20.595 35-39 22.720000000000002 31.314999999999998 25.11 20.855 40-44 19.68 31.115 25.895000000000003 23.31 45-49 19.735 30.14 27.38 22.745 50-54 19.625 32.66 26.615 21.099999999999998 55-59 22.040000000000003 29.959999999999997 24.435000000000002 23.565 60-64 20.244999999999997 31.885 25.4 22.470000000000002 65-69 21.029999999999998 30.009999999999998 25.330000000000002 23.630000000000003 70-74 22.8 30.714999999999996 22.23 24.255 75-79 22.415 30.459999999999997 24.925 22.2 80-84 22.405 29.995 25.019999999999996 22.58 85-89 23.474999999999998 29.32 25.605 21.6 90-94 21.5 30.03 26.605 21.865000000000002 95-99 23.919999999999998 29.29 24.29 22.5 100-104 21.11 30.654999999999998 24.605 23.630000000000003 105-109 21.43 29.13 26.169999999999998 23.27 110-114 22.905 28.13 26.33 22.634999999999998 115-119 20.14 31.014999999999997 26.009999999999998 22.835 120-124 19.5 30.675 25.615 24.21 125-129 20.3 32.05 22.830000000000002 24.82 130-134 22.015 31.09 24.91 21.985 135-139 23.335 29.81 23.16 23.695 140-144 23.990000000000002 29.335 26.14 20.535 145-149 23.03 30.5 25.305 21.165 150 22.325 27.175 28.325 22.175 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.0 21 2.0 22 2.0 23 3.0 24 4.5 25 6.0 26 10.0 27 8.0 28 12.5 29 18.5 30 23.0 31 29.0 32 30.5 33 40.5 34 53.0 35 62.0 36 98.0 37 189.0 38 296.0 39 280.5 40 230.5 41 266.5 42 270.5 43 249.5 44 238.5 45 239.0 46 212.5 47 160.5 48 121.0 49 94.0 50 72.0 51 56.5 52 45.0 53 29.5 54 28.5 55 27.5 56 23.5 57 21.5 58 20.0 59 20.5 60 24.0 61 29.0 62 26.5 63 20.0 64 41.5 65 61.0 66 37.0 67 14.5 68 12.5 69 16.5 70 20.0 71 18.5 72 16.5 73 15.5 74 12.5 75 7.0 76 6.5 77 6.0 78 3.5 79 5.5 80 4.5 81 1.5 82 1.5 83 0.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.625 #Duplication Level Percentage of deduplicated Percentage of total 1 80.47151277013752 51.2 2 12.298624754420432 15.65 3 2.593320235756385 4.95 4 1.2966601178781925 3.3000000000000003 5 0.7858546168958742 2.5 6 0.5893909626719057 2.25 7 0.5108055009823183 2.275 8 0.19646365422396855 1.0 9 0.03929273084479371 0.22499999999999998 >10 1.1787819253438114 13.350000000000001 >50 0.0 0.0 >100 0.03929273084479371 3.3000000000000003 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 132 3.3000000000000003 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 40 1.0 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 38 0.95 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 36 0.8999999999999999 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 35 0.8750000000000001 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 25 0.625 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 25 0.625 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 24 0.6 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 20 0.5 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 18 0.44999999999999996 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 17 0.42500000000000004 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 17 0.42500000000000004 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 16 0.4 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 15 0.375 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 15 0.375 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 14 0.35000000000000003 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 14 0.35000000000000003 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 13 0.325 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 13 0.325 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 13 0.325 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 13 0.325 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 13 0.325 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 12 0.3 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 12 0.3 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 12 0.3 No Hit GGCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGAT 12 0.3 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 11 0.27499999999999997 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 11 0.27499999999999997 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 10 0.25 No Hit TCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATC 10 0.25 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 10 0.25 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 9 0.22499999999999998 No Hit GGGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACA 8 0.2 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 8 0.2 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 8 0.2 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 8 0.2 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 8 0.2 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 7 0.17500000000000002 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 7 0.17500000000000002 No Hit GCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCC 7 0.17500000000000002 No Hit TGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATA 7 0.17500000000000002 No Hit TCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGT 7 0.17500000000000002 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 7 0.17500000000000002 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 7 0.17500000000000002 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 7 0.17500000000000002 No Hit GAGCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTA 7 0.17500000000000002 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 7 0.17500000000000002 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 7 0.17500000000000002 No Hit AGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTAT 7 0.17500000000000002 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 7 0.17500000000000002 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 6 0.15 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 6 0.15 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 6 0.15 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 6 0.15 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 6 0.15 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 6 0.15 No Hit CTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAG 6 0.15 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 6 0.15 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 6 0.15 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 6 0.15 No Hit CCTCCACCAAATTGTAATACAGAATCATCCCCAAAGATTTCGGTCAGAGC 6 0.15 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 6 0.15 No Hit CACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCG 6 0.15 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 6 0.15 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 6 0.15 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 5 0.125 No Hit ACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGA 5 0.125 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 5 0.125 No Hit TGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGG 5 0.125 No Hit ACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 5 0.125 No Hit CCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTT 5 0.125 No Hit GAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATG 5 0.125 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 5 0.125 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 5 0.125 No Hit CATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATATCTACT 5 0.125 No Hit GCCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCAT 5 0.125 No Hit GTCGTGAATAGCTCCGTGGAATAAAATAGAATTTCCTTATGCATAGAACT 5 0.125 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 5 0.125 No Hit CATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAA 5 0.125 No Hit AGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGA 5 0.125 No Hit TCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTT 5 0.125 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 5 0.125 No Hit TAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGA 5 0.125 No Hit GGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGG 5 0.125 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.025 0.0 0.0 0.0 0.0 92-93 0.037500000000000006 0.0 0.0 0.0 0.0 94-95 0.05 0.0 0.0 0.0 0.0 96-97 0.05 0.0 0.0 0.0 0.0 98-99 0.05 0.0 0.0 0.0 0.0 100-101 0.075 0.0 0.0 0.0 0.0 102-103 0.075 0.0 0.0 0.0 0.0 104-105 0.075 0.0 0.0 0.0 0.0 106-107 0.1125 0.0 0.0 0.0 0.0 108-109 0.125 0.0 0.0 0.0 0.0 110-111 0.1875 0.0 0.0 0.0 0.0 112-113 0.2875 0.0 0.0 0.0 0.0 114-115 0.325 0.0 0.0 0.0 0.0 116-117 0.35 0.0 0.0 0.0 0.0 118-119 0.3875 0.0 0.0 0.0 0.0 120-121 0.4 0.0 0.0 0.0 0.0 122-123 0.425 0.0 0.0 0.0 0.0 124-125 0.425 0.0 0.0 0.0 0.0 126-127 0.425 0.0 0.0 0.0 0.0 128-129 0.425 0.0 0.0 0.0 0.0 130-131 0.5 0.0 0.0 0.0 0.0 132-133 0.5 0.0 0.0 0.0 0.0 134-135 0.5 0.0 0.0 0.0 0.0 136-137 0.5 0.0 0.0 0.0 0.0 138 0.5 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGGTCA 35 1.2542517E-4 24.685713 130-134 TGCGGTC 35 1.2542517E-4 24.685713 130-134 TCAATAA 30 0.0015031899 23.999998 135-139 AGGTAGG 30 0.0015031899 23.999998 140-144 AAGTTGC 30 0.0015031899 23.999998 125-129 GCGATGA 35 0.0036813593 20.571428 100-104 ATCTACT 35 0.0036813593 20.571428 85-89 GCGAATA 35 0.0036813593 20.571428 5 TCAATAT 35 0.0036813593 20.571428 80-84 TGGAGGG 35 0.0036813593 20.571428 90-94 CAATATC 35 0.0036813593 20.571428 80-84 CAATAAG 35 0.0036813593 20.571428 135-139 CTGGAGG 35 0.0036813593 20.571428 90-94 CGAATAC 35 0.0036813593 20.571428 6 TGAAGGC 35 0.0036813593 20.571428 105-109 AAGGTAG 35 0.0036813593 20.571428 140-144 GGGGCTG 35 0.0036813593 20.571428 95-99 AATAAAT 35 0.0036813593 20.571428 115-119 GGGCTGC 35 0.0036813593 20.571428 95-99 GAAGGCG 35 0.0036813593 20.571428 105-109 >>END_MODULE ERR11006606 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006606_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.61825 37.0 37.0 37.0 37.0 37.0 2 36.077 37.0 37.0 37.0 37.0 37.0 3 36.125 37.0 37.0 37.0 37.0 37.0 4 36.1235 37.0 37.0 37.0 37.0 37.0 5 36.2535 37.0 37.0 37.0 37.0 37.0 6 36.1095 37.0 37.0 37.0 37.0 37.0 7 36.122 37.0 37.0 37.0 37.0 37.0 8 36.2705 37.0 37.0 37.0 37.0 37.0 9 36.1485 37.0 37.0 37.0 37.0 37.0 10-14 36.2347 37.0 37.0 37.0 37.0 37.0 15-19 36.229200000000006 37.0 37.0 37.0 37.0 37.0 20-24 36.1903 37.0 37.0 37.0 37.0 37.0 25-29 36.1049 37.0 37.0 37.0 37.0 37.0 30-34 36.0575 37.0 37.0 37.0 37.0 37.0 35-39 36.0714 37.0 37.0 37.0 37.0 37.0 40-44 36.083 37.0 37.0 37.0 37.0 37.0 45-49 36.0303 37.0 37.0 37.0 37.0 37.0 50-54 35.9837 37.0 37.0 37.0 37.0 37.0 55-59 35.9424 37.0 37.0 37.0 37.0 37.0 60-64 35.8467 37.0 37.0 37.0 37.0 37.0 65-69 35.8925 37.0 37.0 37.0 37.0 37.0 70-74 35.843900000000005 37.0 37.0 37.0 37.0 37.0 75-79 35.809000000000005 37.0 37.0 37.0 37.0 37.0 80-84 35.712199999999996 37.0 37.0 37.0 37.0 37.0 85-89 35.6409 37.0 37.0 37.0 37.0 37.0 90-94 35.6512 37.0 37.0 37.0 37.0 37.0 95-99 35.569599999999994 37.0 37.0 37.0 37.0 37.0 100-104 35.5101 37.0 37.0 37.0 37.0 37.0 105-109 35.483000000000004 37.0 37.0 37.0 37.0 37.0 110-114 35.3709 37.0 37.0 37.0 34.6 37.0 115-119 35.2682 37.0 37.0 37.0 32.2 37.0 120-124 35.1339 37.0 37.0 37.0 27.4 37.0 125-129 35.199200000000005 37.0 37.0 37.0 27.4 37.0 130-134 35.079499999999996 37.0 37.0 37.0 27.4 37.0 135-139 35.038799999999995 37.0 37.0 37.0 25.0 37.0 140-144 34.9322 37.0 37.0 37.0 25.0 37.0 145-149 34.9269 37.0 37.0 37.0 25.0 37.0 150 34.515 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 2.0 23 3.0 24 15.0 25 11.0 26 18.0 27 15.0 28 27.0 29 34.0 30 39.0 31 70.0 32 92.0 33 117.0 34 216.0 35 627.0 36 2591.0 37 123.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.24369105276574 26.178944685189904 18.429773132806528 27.14759112923783 2 25.575 25.650000000000002 32.85 15.925 3 20.075000000000003 28.449999999999996 33.525 17.95 4 24.175 28.349999999999998 27.525 19.950000000000003 5 24.0 29.475 28.725 17.8 6 20.849999999999998 32.475 27.650000000000002 19.025 7 19.05 19.925 43.1 17.925 8 22.175 24.25 31.25 22.325 9 20.849999999999998 20.724999999999998 35.775 22.650000000000002 10-14 23.200000000000003 26.69 29.74 20.369999999999997 15-19 23.835 25.014999999999997 30.645 20.505000000000003 20-24 24.415 24.32 30.48 20.785 25-29 23.49 25.455 30.919999999999998 20.135 30-34 23.59 24.625 30.305 21.48 35-39 23.31 25.105 30.669999999999998 20.915 40-44 22.225 26.02 30.425 21.33 45-49 22.915 27.655 29.09 20.34 50-54 22.720000000000002 27.29 28.744999999999997 21.245 55-59 23.1 26.07 28.634999999999998 22.195 60-64 22.6 25.55 30.020000000000003 21.83 65-69 23.09 25.130000000000003 29.7 22.08 70-74 23.14 25.705 30.264999999999997 20.89 75-79 23.265 25.314999999999998 30.3 21.12 80-84 24.125 25.374999999999996 29.770000000000003 20.73 85-89 23.84 25.564999999999998 28.24 22.355 90-94 22.85 25.619999999999997 29.315 22.215 95-99 23.265 25.805 29.195 21.735 100-104 23.995 25.16 29.84 21.005 105-109 24.595 24.23 29.48 21.695 110-114 22.735 24.975 29.985 22.305 115-119 23.13 25.729999999999997 30.130000000000003 21.01 120-124 23.73 25.085 29.470000000000002 21.715 125-129 22.31 25.47 30.099999999999998 22.12 130-134 22.25 26.205000000000002 29.57 21.975 135-139 23.405 25.619999999999997 29.909999999999997 21.065 140-144 22.895 24.945 31.19 20.97 145-149 22.009999999999998 24.740000000000002 31.36 21.89 150 22.975 24.5 29.725 22.8 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.5 20 2.0 21 4.0 22 5.5 23 3.5 24 4.0 25 9.0 26 11.0 27 12.5 28 16.0 29 27.0 30 41.0 31 47.0 32 51.0 33 59.5 34 75.5 35 94.0 36 121.0 37 197.0 38 226.0 39 222.5 40 238.0 41 239.5 42 249.0 43 229.5 44 222.5 45 220.0 46 187.5 47 143.5 48 100.0 49 75.0 50 68.5 51 59.0 52 44.0 53 35.0 54 35.5 55 32.5 56 25.5 57 28.0 58 32.5 59 32.0 60 28.5 61 24.5 62 20.5 63 45.0 64 60.5 65 56.0 66 47.0 67 27.0 68 21.0 69 17.0 70 14.5 71 17.5 72 22.5 73 17.0 74 9.5 75 7.5 76 5.0 77 7.0 78 7.0 79 5.0 80 3.5 81 3.0 82 3.5 83 1.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.925 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.60000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 78.73520710059172 53.225 2 12.75887573964497 17.25 3 3.772189349112426 7.6499999999999995 4 1.9230769230769231 5.2 5 0.628698224852071 2.125 6 0.7396449704142012 3.0 7 0.4068047337278107 1.925 8 0.1849112426035503 1.0 9 0.07396449704142012 0.44999999999999996 >10 0.7396449704142012 6.9 >50 0.03698224852071006 1.275 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 51 1.275 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 24 0.6 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 23 0.575 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 18 0.44999999999999996 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 17 0.42500000000000004 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 16 0.4 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 16 0.4 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 15 0.375 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 15 0.375 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 13 0.325 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 13 0.325 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 13 0.325 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 11 0.27499999999999997 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 11 0.27499999999999997 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 11 0.27499999999999997 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 10 0.25 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 10 0.25 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 10 0.25 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 10 0.25 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 10 0.25 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 10 0.25 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 9 0.22499999999999998 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 9 0.22499999999999998 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 8 0.2 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 8 0.2 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 8 0.2 No Hit TATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACC 8 0.2 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 8 0.2 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 7 0.17500000000000002 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 7 0.17500000000000002 No Hit GCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGC 7 0.17500000000000002 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 7 0.17500000000000002 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 7 0.17500000000000002 No Hit ACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTA 7 0.17500000000000002 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 7 0.17500000000000002 No Hit AGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCT 7 0.17500000000000002 No Hit GTTGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTA 7 0.17500000000000002 No Hit GAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTA 7 0.17500000000000002 No Hit CAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTA 7 0.17500000000000002 No Hit GGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTC 6 0.15 No Hit AAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAG 6 0.15 No Hit ATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATAT 6 0.15 No Hit GTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCAT 6 0.15 No Hit AGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCT 6 0.15 No Hit CTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAG 6 0.15 No Hit GGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTT 6 0.15 No Hit CAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGT 6 0.15 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 6 0.15 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 6 0.15 No Hit CTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTAC 6 0.15 No Hit TATTCCTACTTCTGCGGCAATCGGATTGCACTTTTACCCAATTTGGGAAG 6 0.15 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 6 0.15 No Hit TACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGG 6 0.15 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 6 0.15 No Hit AGCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAA 6 0.15 No Hit GCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAA 6 0.15 No Hit GTGGACGTTGCCGTAGCGCTGCGGGCCTGGTCTGGGTGTGCTACTGATGG 6 0.15 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 6 0.15 No Hit GAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGT 6 0.15 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 5 0.125 No Hit CCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTG 5 0.125 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 5 0.125 No Hit GACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCC 5 0.125 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 5 0.125 No Hit CTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCGC 5 0.125 No Hit GTAGCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGT 5 0.125 No Hit GGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTA 5 0.125 No Hit CTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTG 5 0.125 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 5 0.125 No Hit GAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCG 5 0.125 No Hit ATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTAC 5 0.125 No Hit TAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAG 5 0.125 No Hit TATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCAT 5 0.125 No Hit AATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTT 5 0.125 No Hit TGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACT 5 0.125 No Hit GTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.025 0.0 0.0 64-65 0.0 0.0 0.025 0.0 0.0 66-67 0.0 0.0 0.025 0.0 0.0 68-69 0.0 0.0 0.025 0.0 0.0 70-71 0.0 0.0 0.025 0.0 0.0 72-73 0.0 0.0 0.025 0.0 0.0 74-75 0.0 0.0 0.025 0.0 0.0 76-77 0.0 0.0 0.025 0.0 0.0 78-79 0.0 0.0 0.025 0.0 0.0 80-81 0.0 0.0 0.025 0.0 0.0 82-83 0.0 0.0 0.025 0.0 0.0 84-85 0.025 0.0 0.025 0.0 0.0 86-87 0.025 0.0 0.025 0.0 0.0 88-89 0.025 0.0 0.025 0.0 0.0 90-91 0.025 0.0 0.025 0.0 0.0 92-93 0.037500000000000006 0.0 0.025 0.0 0.0 94-95 0.05 0.0 0.025 0.0 0.0 96-97 0.05 0.0 0.025 0.0 0.0 98-99 0.05 0.0 0.025 0.0 0.0 100-101 0.075 0.0 0.025 0.0 0.0 102-103 0.075 0.0 0.025 0.0 0.0 104-105 0.075 0.0 0.025 0.0 0.0 106-107 0.0875 0.0 0.025 0.0 0.0 108-109 0.1 0.0 0.025 0.0 0.0 110-111 0.16249999999999998 0.0 0.025 0.0 0.0 112-113 0.2625 0.0 0.025 0.0 0.0 114-115 0.3 0.0 0.025 0.0 0.0 116-117 0.325 0.0 0.025 0.0 0.0 118-119 0.3625 0.0 0.025 0.0 0.0 120-121 0.375 0.0 0.025 0.0 0.0 122-123 0.4 0.0 0.025 0.0 0.0 124-125 0.4 0.0 0.025 0.0 0.0 126-127 0.4 0.0 0.025 0.0 0.0 128-129 0.4 0.0 0.025 0.0 0.0 130-131 0.475 0.0 0.025 0.0 0.0 132-133 0.475 0.0 0.025 0.0 0.0 134-135 0.475 0.0 0.025 0.0 0.0 136-137 0.475 0.0 0.025 0.0 0.0 138 0.475 0.0 0.025 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGATA 10 0.0069754543 143.9875 5 >>END_MODULE Read 2574194 spots for ERR11006606.sra Written 2574194 spots for ERR11006606.sra Read 2574194 spots for ERR11006606.sra Written 2574194 spots for ERR11006606.sra Read 2574194 spots for ERR11006606.sra Written 2574194 spots for ERR11006606.sra Read 2574194 spots for ERR11006606.sra Written 2574194 spots for ERR11006606.sra Read 2574194 spots for ERR11006606.sra Written 2574194 spots for ERR11006606.sra Read 2574194 spots for ERR11006606.sra Written 2574194 spots for ERR11006606.sra Read 2574194 spots for ERR11006606.sra Written 2574194 spots for ERR11006606.sra Read 2574194 spots for ERR11006606.sra Written 2574194 spots for ERR11006606.sra Read 2574212 spots for ERR11006606.sra Written 2574212 spots for ERR11006606.sra Read 2574194 spots for ERR11006606.sra Written 2574194 spots for ERR11006606.sra Read 2574194 spots for ERR11006606.sra Written 2574194 spots for ERR11006606.sra Read 2574194 spots for ERR11006606.sra Written 2574194 spots for ERR11006606.sra Read 2574194 spots for ERR11006606.sra Written 2574194 spots for ERR11006606.sra Read 2574194 spots for ERR11006606.sra Written 2574194 spots for ERR11006606.sra Read 2574194 spots for ERR11006606.sra Written 2574194 spots for ERR11006606.sra Read 2574194 spots for ERR11006606.sra Written 2574194 spots for ERR11006606.sra Read 2574194 spots for ERR11006606.sra Written 2574194 spots for ERR11006606.sra Read 2574194 spots for ERR11006606.sra Written 2574194 spots for ERR11006606.sra Read 2574194 spots for ERR11006606.sra Written 2574194 spots for ERR11006606.sra Read 2574194 spots for ERR11006606.sra Written 2574194 spots for ERR11006606.sra SRR ids: ['ERR11006606.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd__0tqk2me ERR11006606.sra spots: 51483898 blocks: [[1, 2574194], [2574195, 5148388], [5148389, 7722582], [7722583, 10296776], [10296777, 12870970], [12870971, 15445164], [15445165, 18019358], [18019359, 20593552], [20593553, 23167746], [23167747, 25741940], [25741941, 28316134], [28316135, 30890328], [30890329, 33464522], [33464523, 36038716], [36038717, 38612910], [38612911, 41187104], [41187105, 43761298], [43761299, 46335492], [46335493, 48909686], [48909687, 51483898]] ERR11006606 file size 18916825 ERR11006606 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006606 ERR11006606_1.fastq ERR11006606_2.fastq Input file: ERR11006606_1.fastq Paired file: ERR11006606_2.fastq trimmed: ERR11006606-trimmed-pair1.fastq, ERR11006606-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 19:43:38 2024 >> started Fri Dec 6 19:44:46 2024 >> done (68.083s) 51483898 read pairs processed; of these: 193 ( 0.00%) short read pairs filtered out after trimming by size control 669 ( 0.00%) empty read pairs filtered out after trimming by size control 51483036 (100.00%) read pairs available; of these: 373564 ( 0.73%) trimmed read pairs available after processing 51109472 (99.27%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 11 0.00% 19 7 0.00% 20 0 0.00% 21 21 0.00% 22 24 0.00% 23 9 0.00% 24 9 0.00% 25 22 0.00% 26 9 0.00% 27 7 0.00% 28 13 0.00% 29 17 0.00% 30 16 0.00% 31 51 0.00% 32 9 0.00% 33 14 0.00% 34 12 0.00% 35 19 0.00% 36 19 0.00% 37 16 0.00% 38 19 0.00% 39 19 0.00% 40 13 0.00% 41 17 0.00% 42 19 0.00% 43 22 0.00% 44 23 0.00% 45 25 0.00% 46 23 0.00% 47 28 0.00% 48 21 0.00% 49 28 0.00% 50 46 0.00% 51 45 0.00% 52 53 0.00% 53 46 0.00% 54 55 0.00% 55 43 0.00% 56 74 0.00% 57 59 0.00% 58 63 0.00% 59 73 0.00% 60 97 0.00% 61 115 0.00% 62 111 0.00% 63 133 0.00% 64 157 0.00% 65 184 0.00% 66 172 0.00% 67 167 0.00% 68 186 0.00% 69 189 0.00% 70 193 0.00% 71 228 0.00% 72 300 0.00% 73 298 0.00% 74 276 0.00% 75 325 0.00% 76 356 0.00% 77 410 0.00% 78 462 0.00% 79 388 0.00% 80 480 0.00% 81 489 0.00% 82 551 0.00% 83 618 0.00% 84 586 0.00% 85 715 0.00% 86 741 0.00% 87 821 0.00% 88 905 0.00% 89 884 0.00% 90 1003 0.00% 91 1028 0.00% 92 1099 0.00% 93 1160 0.00% 94 1206 0.00% 95 1348 0.00% 96 1425 0.00% 97 1571 0.00% 98 1735 0.00% 99 1746 0.00% 100 1830 0.00% 101 1823 0.00% 102 2016 0.00% 103 2102 0.00% 104 2189 0.00% 105 2394 0.00% 106 2475 0.00% 107 2558 0.00% 108 2760 0.01% 109 2847 0.01% 110 3066 0.01% 111 3249 0.01% 112 3617 0.01% 113 3495 0.01% 114 3515 0.01% 115 3725 0.01% 116 4148 0.01% 117 4334 0.01% 118 4364 0.01% 119 4760 0.01% 120 4975 0.01% 121 5226 0.01% 122 5679 0.01% 123 5948 0.01% 124 6204 0.01% 125 7018 0.01% 126 6330 0.01% 127 6338 0.01% 128 6645 0.01% 129 7342 0.01% 130 7707 0.01% 131 7977 0.02% 132 8574 0.02% 133 8167 0.02% 134 8652 0.02% 135 8504 0.02% 136 9187 0.02% 137 9386 0.02% 138 9724 0.02% 139 10491 0.02% 140 10402 0.02% 141 11260 0.02% 142 11805 0.02% 143 12357 0.02% 144 12753 0.02% 145 13845 0.03% 146 16359 0.03% 147 15325 0.03% 148 15846 0.03% 149 16294 0.03% 150 51109472 99.27% 51483036 reads passed initial QC criterion=sequence-density sequence-density=0.35 sequence-density-rank=1 fanout-score=2.05 fanout-score-rank=26 prefix-density=0.36 prefix-fanout=2.0 sequence=TAGGAGAGCCGC criterion=fanout-score sequence-density=0.02 sequence-density-rank=29 fanout-score=30.47 fanout-score-rank=1 prefix-density=0.31 prefix-fanout=2.0 sequence=AAAAACAGTAGAAGTAGAACAGGTATAAATAAGAAAATCTTAGTTAAGAGGGTTCATGTAAAGAACAGGTTCTAAATCACGATCGATTCCCTTTTCAAAACCTGCTGCAGCAGCTCGGGCTCTTCCTGCATGCCACAAATGGCCCACAAAAAAGAAGAATCCTAGAACAAAATGAGAAGTCGATAACCAACTTCTAGGAGAGACATAATTAACTGCATTGATCTCGGTAGCTACGCCACCCACGGAATTTAAAGAGCCTAAAGGAGCATGGGTCATATATTCCGCTGAACGTCGTTCTTGCCAAGGTTGTATGTCTTTTTTCAACCTACTCAAGTCCAAACCGTTGGGCCCCCTTAGAGGTTCTAACCATGGAGCACGGAGGTCCCAAAAACGCATAGTTTCCCCTCCAAAGATAACCTCTCCCGTTGGGGAACGCATTAGATATTTACCTAAACCTGTGGGTCCTTGAGCAGATCCCACATTAGCTCCAAGACGCTGGTCTCTAACTAGA criterion=sequence-density sequence-density=0.50 sequence-density-rank=1 fanout-score=4.59 fanout-score-rank=21 prefix-density=0.77 prefix-fanout=3.0 sequence=TGGGGAAGAGGA criterion=fanout-score sequence-density=0.09 sequence-density-rank=17 fanout-score=110.83 fanout-score-rank=1 prefix-density=10.14 prefix-fanout=1.0 sequence=CTGGATAACTATCACTGAAAATC ERR11006606 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 19:45:22 Started mapping on | Dec 06 19:45:22 Finished on | Dec 06 19:52:24 Mapping speed, Million of reads per hour | 439.19 Number of input reads | 51483036 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 37780894 Uniquely mapped reads % | 73.39% Average mapped length | 298.37 Number of splices: Total | 12317899 Number of splices: Annotated (sjdb) | 11436464 Number of splices: GT/AG | 11960571 Number of splices: GC/AG | 149904 Number of splices: AT/AC | 24774 Number of splices: Non-canonical | 182650 Mismatch rate per base, % | 0.31% Deletion rate per base | 0.01% Deletion average length | 1.75 Insertion rate per base | 0.01% Insertion average length | 2.11 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 11804032 % of reads mapped to multiple loci | 22.93% Number of reads mapped to too many loci | 8127 % of reads mapped to too many loci | 0.02% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.42% % of reads unmapped: other | 0.25% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1898110 1898110 1898110 N_multimapping 11804032 11804032 11804032 N_noFeature 9640640 34818173 11566574 N_ambiguous 1834003 46506 819531 UnstrandedReadsAssigned:26306251 PositiveStrandReadsAssigned:2916215 NegativeStrandReadsAssigned:25394789 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006606 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006606-trimmed-pair1.fastq ERR11006606-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 51,483,036 reads, 29,169,910 reads pseudoaligned [quant] estimated average fragment length: 283.059 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,114 rounds 52973 ERR11006606.ke.tsv 35125 ERR11006606.se.tsv 88098 total ==> ERR11006606.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 654.462 0 0 PNS24247 1044 761.941 8.4387 0.355249 PNS24249 1928 1645.94 103.523 2.01745 PNS24246 1044 761.941 8.4387 0.355249 PNS24248 1044 761.941 8.4387 0.355249 PNS24244 1471 1188.94 43.1605 1.1644 PNS24243 293 50.0352 0 0 KQK14069 1603 1320.94 2639.52 64.0943 KQK14071 474 195.212 43.3689 7.12605 ==> ERR11006606.se.tsv <== BRADI_1g14170v3 2981 BRADI_1g53295v3 85 BRADI_1g59795v3 153 BRADI_1g07683v3 0 BRADI_1g00485v3 6 BRADI_1g20270v3 182 BRADI_1g74790v3 182 BRADI_1g09890v3 0 BRADI_1g77505v3 166 BRADI_1g48960v3 0 ERR11006606 completed mapping pipeline successfully