Starting /dee2/code/volunteer_pipeline.sh ERR11006607 current disk space = 1549714268160 free memory = 1597953136 ERR11006607 SRAfilesize 5c32741f9db2c7a06e0fe117c79fc3b7 ERR11006607.sra ERR11006607.sra file validated ERR11006607 is paired end ERR11006607 is conventional basespace ERR11006607 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006607_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.3645 37.0 37.0 37.0 37.0 37.0 2 36.1725 37.0 37.0 37.0 37.0 37.0 3 36.282 37.0 37.0 37.0 37.0 37.0 4 36.467 37.0 37.0 37.0 37.0 37.0 5 36.346 37.0 37.0 37.0 37.0 37.0 6 36.533 37.0 37.0 37.0 37.0 37.0 7 36.429 37.0 37.0 37.0 37.0 37.0 8 36.451 37.0 37.0 37.0 37.0 37.0 9 36.398 37.0 37.0 37.0 37.0 37.0 10-14 36.43 37.0 37.0 37.0 37.0 37.0 15-19 36.433 37.0 37.0 37.0 37.0 37.0 20-24 36.3722 37.0 37.0 37.0 37.0 37.0 25-29 36.315999999999995 37.0 37.0 37.0 37.0 37.0 30-34 36.3059 37.0 37.0 37.0 37.0 37.0 35-39 36.3138 37.0 37.0 37.0 37.0 37.0 40-44 36.213499999999996 37.0 37.0 37.0 37.0 37.0 45-49 36.1959 37.0 37.0 37.0 37.0 37.0 50-54 36.211 37.0 37.0 37.0 37.0 37.0 55-59 36.17190000000001 37.0 37.0 37.0 37.0 37.0 60-64 36.1301 37.0 37.0 37.0 37.0 37.0 65-69 36.1545 37.0 37.0 37.0 37.0 37.0 70-74 36.12669999999999 37.0 37.0 37.0 37.0 37.0 75-79 36.078 37.0 37.0 37.0 37.0 37.0 80-84 35.990300000000005 37.0 37.0 37.0 37.0 37.0 85-89 35.9963 37.0 37.0 37.0 37.0 37.0 90-94 35.9039 37.0 37.0 37.0 37.0 37.0 95-99 36.0386 37.0 37.0 37.0 37.0 37.0 100-104 35.907799999999995 37.0 37.0 37.0 37.0 37.0 105-109 35.8247 37.0 37.0 37.0 37.0 37.0 110-114 35.7429 37.0 37.0 37.0 37.0 37.0 115-119 35.7525 37.0 37.0 37.0 37.0 37.0 120-124 35.7504 37.0 37.0 37.0 37.0 37.0 125-129 35.66329999999999 37.0 37.0 37.0 37.0 37.0 130-134 35.5398 37.0 37.0 37.0 37.0 37.0 135-139 35.500899999999994 37.0 37.0 37.0 37.0 37.0 140-144 35.3048 37.0 37.0 37.0 34.6 37.0 145-149 35.4932 37.0 37.0 37.0 37.0 37.0 150 35.169 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 0.0 23 2.0 24 1.0 25 4.0 26 10.0 27 14.0 28 20.0 29 34.0 30 65.0 31 55.0 32 88.0 33 97.0 34 141.0 35 325.0 36 2808.0 37 335.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.2 12.125 15.775 34.9 2 29.849999999999998 10.9 26.1 33.15 3 22.625 12.975 26.674999999999997 37.724999999999994 4 26.424999999999997 16.85 25.324999999999996 31.4 5 28.050000000000004 19.3 26.625 26.025 6 25.7 29.45 21.475 23.375 7 16.525000000000002 31.8 33.4 18.275 8 17.575 29.975 32.45 20.0 9 17.7 28.549999999999997 34.849999999999994 18.9 10-14 20.145 32.26 26.61 20.985 15-19 19.96 34.035 24.855 21.15 20-24 19.259999999999998 29.205 27.750000000000004 23.785 25-29 22.155 32.015 25.419999999999998 20.41 30-34 23.52 31.135 24.725 20.62 35-39 22.7 31.97 24.585 20.745 40-44 20.294999999999998 30.904999999999998 25.224999999999998 23.575 45-49 20.555 30.035 26.784999999999997 22.625 50-54 19.66 32.934999999999995 25.45 21.955 55-59 22.46 30.545 22.915 24.08 60-64 19.97 32.08 24.9 23.05 65-69 21.240000000000002 29.985 25.19 23.585 70-74 23.145 29.87 22.445 24.54 75-79 22.68 30.395 24.595 22.33 80-84 22.134999999999998 29.995 24.05 23.82 85-89 23.380000000000003 29.56 24.915000000000003 22.145 90-94 21.39 29.975 26.174999999999997 22.46 95-99 24.32 29.515 23.105 23.06 100-104 22.125 29.799999999999997 24.355 23.72 105-109 21.95 28.715000000000003 25.945 23.39 110-114 23.73 28.315 24.695 23.26 115-119 20.169999999999998 30.84 25.82 23.169999999999998 120-124 19.805 30.56 24.715 24.92 125-129 20.4 31.974999999999998 22.505 25.119999999999997 130-134 22.84 30.349999999999998 24.84 21.97 135-139 23.400000000000002 29.470000000000002 23.105 24.025 140-144 23.71 29.32 26.0 20.97 145-149 22.785 30.615 24.92 21.68 150 22.775000000000002 27.075 28.95 21.2 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.0 5 1.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 1.5 23 3.5 24 4.5 25 7.5 26 7.0 27 5.0 28 11.5 29 19.0 30 19.5 31 18.5 32 26.5 33 45.5 34 53.0 35 56.0 36 92.0 37 163.0 38 263.0 39 273.5 40 246.0 41 283.5 42 273.0 43 251.0 44 226.5 45 214.0 46 203.5 47 153.0 48 113.5 49 89.0 50 80.0 51 68.5 52 49.5 53 37.0 54 34.5 55 29.5 56 34.0 57 35.5 58 28.0 59 26.0 60 26.0 61 27.5 62 25.5 63 23.5 64 47.0 65 67.0 66 43.5 67 23.0 68 27.0 69 28.0 70 21.0 71 15.5 72 12.5 73 11.5 74 10.0 75 9.0 76 7.0 77 5.5 78 6.5 79 5.0 80 3.0 81 2.0 82 1.0 83 1.5 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.45 #Duplication Level Percentage of deduplicated Percentage of total 1 80.64391000775795 51.975 2 12.102404965089217 15.6 3 3.1031807602792862 6.0 4 1.2412723041117144 3.2 5 0.6982156710628394 2.25 6 0.3491078355314197 1.35 7 0.27152831652443754 1.225 8 0.3491078355314197 1.7999999999999998 9 0.11636927851047324 0.675 >10 1.0861132660977502 13.100000000000001 >50 0.0 0.0 >100 0.038789759503491075 2.825 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 113 2.825 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 45 1.125 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 39 0.975 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 33 0.8250000000000001 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 31 0.775 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 28 0.7000000000000001 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 23 0.575 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 23 0.575 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 22 0.5499999999999999 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 21 0.525 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 21 0.525 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 18 0.44999999999999996 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 16 0.4 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 16 0.4 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 15 0.375 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 14 0.35000000000000003 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 14 0.35000000000000003 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 14 0.35000000000000003 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 14 0.35000000000000003 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 13 0.325 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 13 0.325 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 13 0.325 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 12 0.3 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 12 0.3 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 11 0.27499999999999997 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 11 0.27499999999999997 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 11 0.27499999999999997 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 11 0.27499999999999997 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 10 0.25 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 9 0.22499999999999998 No Hit CCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTT 9 0.22499999999999998 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 9 0.22499999999999998 No Hit GGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAA 8 0.2 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 8 0.2 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 8 0.2 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 8 0.2 No Hit ATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATA 8 0.2 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 8 0.2 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 8 0.2 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 8 0.2 No Hit GCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAA 8 0.2 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 7 0.17500000000000002 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 7 0.17500000000000002 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 7 0.17500000000000002 No Hit CAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAA 7 0.17500000000000002 No Hit CCCACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACA 7 0.17500000000000002 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 7 0.17500000000000002 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 7 0.17500000000000002 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 6 0.15 No Hit GCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAA 6 0.15 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 6 0.15 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 6 0.15 No Hit GGCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGAT 6 0.15 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 6 0.15 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 6 0.15 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 6 0.15 No Hit CACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAAT 6 0.15 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 5 0.125 No Hit GACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGG 5 0.125 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 5 0.125 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 5 0.125 No Hit GCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCC 5 0.125 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 5 0.125 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 5 0.125 No Hit CACCCAGTTCCCCAGCCCCAGCGCCTCCGGCACCAACACCCCTGGCACGC 5 0.125 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 5 0.125 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 5 0.125 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 5 0.125 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 5 0.125 No Hit GGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGA 5 0.125 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 5 0.125 No Hit AGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTAT 5 0.125 No Hit TCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTT 5 0.125 No Hit CGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGG 5 0.125 No Hit GGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.025 0.0 0.0 0.0 0.0 92-93 0.025 0.0 0.0 0.0 0.0 94-95 0.025 0.0 0.0 0.0 0.0 96-97 0.025 0.0 0.0 0.0 0.0 98-99 0.025 0.0 0.0 0.0 0.0 100-101 0.075 0.0 0.0 0.0 0.0 102-103 0.1 0.0 0.0 0.0 0.0 104-105 0.1 0.0 0.0 0.0 0.0 106-107 0.1 0.0 0.0 0.0 0.0 108-109 0.125 0.0 0.0 0.0 0.0 110-111 0.125 0.0 0.0 0.0 0.0 112-113 0.125 0.0 0.0 0.0 0.0 114-115 0.125 0.0 0.0 0.0 0.0 116-117 0.15 0.0 0.0 0.0 0.0 118-119 0.15 0.0 0.0 0.0 0.0 120-121 0.2 0.0 0.0 0.0 0.0 122-123 0.2 0.0 0.0 0.0 0.0 124-125 0.2 0.0 0.0 0.0 0.0 126-127 0.225 0.0 0.0 0.0 0.0 128-129 0.225 0.0 0.0 0.0 0.0 130-131 0.225 0.0 0.0 0.0 0.0 132-133 0.275 0.0 0.0 0.0 0.0 134-135 0.325 0.0 0.0 0.0 0.0 136-137 0.35 0.0 0.0 0.0 0.0 138 0.375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGTTCG 10 0.006973645 144.0 3 GGTTCGC 10 0.006973645 144.0 4 TTTTATT 10 0.006973645 144.0 7 GGTGGTT 10 0.006973645 144.0 1 GTGGTTC 10 0.006973645 144.0 2 CATAAGG 40 0.005777437 54.0 5 ATAAGGA 45 0.009205684 48.0 6 >>END_MODULE ERR11006607 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006607_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.56375 37.0 37.0 37.0 37.0 37.0 2 36.16 37.0 37.0 37.0 37.0 37.0 3 36.302 37.0 37.0 37.0 37.0 37.0 4 36.16 37.0 37.0 37.0 37.0 37.0 5 36.372 37.0 37.0 37.0 37.0 37.0 6 36.2855 37.0 37.0 37.0 37.0 37.0 7 36.2725 37.0 37.0 37.0 37.0 37.0 8 36.332 37.0 37.0 37.0 37.0 37.0 9 36.254 37.0 37.0 37.0 37.0 37.0 10-14 36.370000000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.405 37.0 37.0 37.0 37.0 37.0 20-24 36.2833 37.0 37.0 37.0 37.0 37.0 25-29 36.2632 37.0 37.0 37.0 37.0 37.0 30-34 36.2291 37.0 37.0 37.0 37.0 37.0 35-39 36.2631 37.0 37.0 37.0 37.0 37.0 40-44 36.1833 37.0 37.0 37.0 37.0 37.0 45-49 36.208 37.0 37.0 37.0 37.0 37.0 50-54 36.15240000000001 37.0 37.0 37.0 37.0 37.0 55-59 36.1245 37.0 37.0 37.0 37.0 37.0 60-64 35.991 37.0 37.0 37.0 37.0 37.0 65-69 36.0332 37.0 37.0 37.0 37.0 37.0 70-74 36.042500000000004 37.0 37.0 37.0 37.0 37.0 75-79 35.980399999999996 37.0 37.0 37.0 37.0 37.0 80-84 35.8964 37.0 37.0 37.0 37.0 37.0 85-89 35.8536 37.0 37.0 37.0 37.0 37.0 90-94 35.852500000000006 37.0 37.0 37.0 37.0 37.0 95-99 35.6853 37.0 37.0 37.0 37.0 37.0 100-104 35.6798 37.0 37.0 37.0 37.0 37.0 105-109 35.621900000000004 37.0 37.0 37.0 37.0 37.0 110-114 35.5317 37.0 37.0 37.0 37.0 37.0 115-119 35.4939 37.0 37.0 37.0 37.0 37.0 120-124 35.3334 37.0 37.0 37.0 34.6 37.0 125-129 35.388 37.0 37.0 37.0 37.0 37.0 130-134 35.3007 37.0 37.0 37.0 32.2 37.0 135-139 35.179 37.0 37.0 37.0 25.0 37.0 140-144 35.05069999999999 37.0 37.0 37.0 25.0 37.0 145-149 35.213300000000004 37.0 37.0 37.0 27.4 37.0 150 34.7955 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 4.0 22 0.0 23 2.0 24 13.0 25 8.0 26 16.0 27 6.0 28 25.0 29 21.0 30 39.0 31 49.0 32 70.0 33 120.0 34 194.0 35 544.0 36 2680.0 37 208.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.804021379485874 25.375413591244588 16.13642148129295 28.684143547976582 2 26.825 25.75 31.2 16.225 3 20.825 28.9 32.025 18.25 4 23.775 28.249999999999996 28.000000000000004 19.975 5 22.8 29.525000000000002 29.049999999999997 18.625 6 20.05 32.525 28.599999999999998 18.825 7 19.3 22.15 39.75 18.8 8 21.725 22.225 32.05 24.0 9 23.75 19.75 34.050000000000004 22.45 10-14 23.595 25.619999999999997 29.14 21.645 15-19 23.435 25.045 30.53 20.990000000000002 20-24 24.709999999999997 24.43 30.409999999999997 20.45 25-29 24.39 24.779999999999998 29.78 21.05 30-34 24.709999999999997 24.610000000000003 30.03 20.65 35-39 24.875 24.125 29.9 21.099999999999998 40-44 23.56 25.674999999999997 29.875 20.89 45-49 23.169999999999998 26.935 28.52 21.375 50-54 23.31 25.705 28.95 22.035 55-59 23.635 24.98 28.74 22.645 60-64 22.925 24.425 29.995 22.655 65-69 23.385 24.425 29.445 22.745 70-74 24.15 25.174999999999997 29.535 21.14 75-79 23.66 24.59 30.825000000000003 20.925 80-84 24.505 24.349999999999998 29.82 21.325 85-89 24.63 25.374999999999996 28.194999999999997 21.8 90-94 23.830000000000002 24.935 29.054999999999996 22.18 95-99 23.485 24.45 29.68 22.384999999999998 100-104 23.96 23.775 30.659999999999997 21.605 105-109 25.5 23.405 30.25 20.845 110-114 23.905 23.745 29.955 22.395 115-119 23.645 24.36 30.070000000000004 21.925 120-124 23.355 25.740000000000002 28.83 22.075 125-129 23.07 25.2 30.085 21.645 130-134 22.98 25.95 29.145 21.925 135-139 23.549999999999997 25.019999999999996 30.695 20.735 140-144 23.74 25.145 29.755 21.36 145-149 21.92 24.55 31.47 22.06 150 22.7 22.75 29.625 24.925 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.5 17 1.5 18 2.5 19 2.5 20 0.0 21 1.0 22 3.0 23 3.0 24 4.0 25 8.5 26 10.5 27 11.5 28 20.0 29 27.5 30 31.5 31 32.5 32 32.5 33 37.5 34 49.0 35 68.0 36 117.5 37 190.5 38 211.5 39 209.5 40 241.0 41 246.5 42 230.0 43 232.0 44 245.0 45 237.0 46 204.5 47 162.0 48 114.0 49 76.0 50 59.5 51 60.5 52 49.0 53 32.0 54 27.5 55 27.0 56 28.0 57 32.0 58 31.5 59 28.5 60 30.0 61 27.0 62 22.0 63 56.0 64 73.0 65 46.5 66 33.5 67 38.0 68 40.0 69 33.5 70 29.0 71 23.5 72 22.5 73 20.0 74 15.5 75 13.5 76 10.5 77 7.0 78 5.5 79 6.5 80 4.5 81 1.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.775 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.55 #Duplication Level Percentage of deduplicated Percentage of total 1 78.49740932642487 53.025 2 12.657290895632864 17.1 3 3.9970392301998516 8.1 4 2.072538860103627 5.6000000000000005 5 0.8142116950407106 2.75 6 0.5921539600296076 2.4 7 0.1850481125092524 0.8750000000000001 8 0.2590673575129534 1.4000000000000001 9 0.2960769800148038 1.7999999999999998 >10 0.6291635825314582 6.950000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 41 1.0250000000000001 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 23 0.575 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 21 0.525 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 21 0.525 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 19 0.475 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 19 0.475 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 17 0.42500000000000004 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 15 0.375 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 15 0.375 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 13 0.325 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 12 0.3 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 11 0.27499999999999997 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 11 0.27499999999999997 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 10 0.25 No Hit GAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCG 10 0.25 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 10 0.25 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 10 0.25 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 9 0.22499999999999998 No Hit CTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAG 9 0.22499999999999998 No Hit CCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGA 9 0.22499999999999998 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 9 0.22499999999999998 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 9 0.22499999999999998 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 9 0.22499999999999998 No Hit CTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGG 9 0.22499999999999998 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 9 0.22499999999999998 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 8 0.2 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 8 0.2 No Hit GATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTC 8 0.2 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 8 0.2 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 8 0.2 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 8 0.2 No Hit AACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGG 8 0.2 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 7 0.17500000000000002 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 7 0.17500000000000002 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 7 0.17500000000000002 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 7 0.17500000000000002 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 7 0.17500000000000002 No Hit GAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGAC 6 0.15 No Hit GTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTT 6 0.15 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 6 0.15 No Hit AATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTAT 6 0.15 No Hit ATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATAT 6 0.15 No Hit TCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGA 6 0.15 No Hit ATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGA 6 0.15 No Hit TGCATATTCAGCTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACC 6 0.15 No Hit CTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTTATGA 6 0.15 No Hit TGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTACTGAAAATGAAT 6 0.15 No Hit GCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAG 6 0.15 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 6 0.15 No Hit TGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACT 6 0.15 No Hit TCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTG 6 0.15 No Hit ATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTG 6 0.15 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 6 0.15 No Hit AAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATC 5 0.125 No Hit AAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAA 5 0.125 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 5 0.125 No Hit CTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCT 5 0.125 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 5 0.125 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 5 0.125 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 5 0.125 No Hit CTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTA 5 0.125 No Hit AGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAG 5 0.125 No Hit AATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGT 5 0.125 No Hit ATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATATT 5 0.125 No Hit CTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGTGGGAACTTAGTT 5 0.125 No Hit CAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTA 5 0.125 No Hit ATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTG 5 0.125 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 5 0.125 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 5 0.125 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 5 0.125 No Hit CCTCGACGGGTCCTCGCCCGGCGACTTCGGGTTCGACCCCCTCGGCCTCG 5 0.125 No Hit ATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAG 5 0.125 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 5 0.125 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 5 0.125 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.05 0.0 0.0 0.0 0.0 84-85 0.05 0.0 0.0 0.0 0.0 86-87 0.05 0.0 0.0 0.0 0.0 88-89 0.075 0.0 0.0 0.0 0.0 90-91 0.075 0.0 0.0 0.0 0.0 92-93 0.075 0.0 0.0 0.0 0.0 94-95 0.075 0.0 0.0 0.0 0.0 96-97 0.075 0.0 0.0 0.0 0.0 98-99 0.075 0.0 0.0 0.0 0.0 100-101 0.125 0.0 0.0 0.0 0.0 102-103 0.15 0.0 0.0 0.0 0.0 104-105 0.15 0.0 0.0 0.0 0.0 106-107 0.15 0.0 0.0 0.0 0.0 108-109 0.175 0.0 0.0 0.0 0.0 110-111 0.175 0.0 0.0 0.0 0.0 112-113 0.175 0.0 0.0 0.0 0.0 114-115 0.175 0.0 0.0 0.0 0.0 116-117 0.2 0.0 0.0 0.0 0.0 118-119 0.2 0.0 0.0 0.0 0.0 120-121 0.25 0.0 0.0 0.0 0.0 122-123 0.25 0.0 0.0 0.0 0.0 124-125 0.25 0.0 0.0 0.0 0.0 126-127 0.275 0.0 0.0 0.0 0.0 128-129 0.275 0.0 0.0 0.0 0.0 130-131 0.275 0.0 0.0 0.0 0.0 132-133 0.325 0.0 0.0 0.0 0.0 134-135 0.375 0.0 0.0 0.0 0.0 136-137 0.3875 0.0 0.0 0.0 0.0 138 0.4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 2648263 spots for ERR11006607.sra Written 2648263 spots for ERR11006607.sra Read 2648263 spots for ERR11006607.sra Written 2648263 spots for ERR11006607.sra Read 2648263 spots for ERR11006607.sra Written 2648263 spots for ERR11006607.sra Read 2648263 spots for ERR11006607.sra Written 2648263 spots for ERR11006607.sra Read 2648263 spots for ERR11006607.sra Written 2648263 spots for ERR11006607.sra Read 2648263 spots for ERR11006607.sra Written 2648263 spots for ERR11006607.sra Read 2648273 spots for ERR11006607.sra Written 2648273 spots for ERR11006607.sra Read 2648263 spots for ERR11006607.sra Written 2648263 spots for ERR11006607.sra Read 2648263 spots for ERR11006607.sra Written 2648263 spots for ERR11006607.sra Read 2648263 spots for ERR11006607.sra Written 2648263 spots for ERR11006607.sra Read 2648263 spots for ERR11006607.sra Written 2648263 spots for ERR11006607.sra Read 2648263 spots for ERR11006607.sra Written 2648263 spots for ERR11006607.sra Read 2648263 spots for ERR11006607.sra Written 2648263 spots for ERR11006607.sra Read 2648263 spots for ERR11006607.sra Written 2648263 spots for ERR11006607.sra Read 2648263 spots for ERR11006607.sra Written 2648263 spots for ERR11006607.sra Read 2648263 spots for ERR11006607.sra Written 2648263 spots for ERR11006607.sra Read 2648263 spots for ERR11006607.sra Written 2648263 spots for ERR11006607.sra Read 2648263 spots for ERR11006607.sra Written 2648263 spots for ERR11006607.sra Read 2648263 spots for ERR11006607.sra Written 2648263 spots for ERR11006607.sra Read 2648263 spots for ERR11006607.sra Written 2648263 spots for ERR11006607.sra SRR ids: ['ERR11006607.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_m775_8ll ERR11006607.sra spots: 52965270 blocks: [[1, 2648263], [2648264, 5296526], [5296527, 7944789], [7944790, 10593052], [10593053, 13241315], [13241316, 15889578], [15889579, 18537841], [18537842, 21186104], [21186105, 23834367], [23834368, 26482630], [26482631, 29130893], [29130894, 31779156], [31779157, 34427419], [34427420, 37075682], [37075683, 39723945], [39723946, 42372208], [42372209, 45020471], [45020472, 47668734], [47668735, 50316997], [50316998, 52965270]] ERR11006607 file size 19461451 ERR11006607 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006607 ERR11006607_1.fastq ERR11006607_2.fastq Input file: ERR11006607_1.fastq Paired file: ERR11006607_2.fastq trimmed: ERR11006607-trimmed-pair1.fastq, ERR11006607-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 19:45:21 2024 >> started Fri Dec 6 19:46:23 2024 >> done (62.355s) 52965270 read pairs processed; of these: 88 ( 0.00%) short read pairs filtered out after trimming by size control 799 ( 0.00%) empty read pairs filtered out after trimming by size control 52964383 (100.00%) read pairs available; of these: 441835 ( 0.83%) trimmed read pairs available after processing 52522548 (99.17%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 4 0.00% 20 3 0.00% 21 10 0.00% 22 18 0.00% 23 5 0.00% 24 8 0.00% 25 21 0.00% 26 6 0.00% 27 15 0.00% 28 14 0.00% 29 15 0.00% 30 14 0.00% 31 55 0.00% 32 16 0.00% 33 11 0.00% 34 5 0.00% 35 15 0.00% 36 13 0.00% 37 17 0.00% 38 18 0.00% 39 18 0.00% 40 17 0.00% 41 24 0.00% 42 27 0.00% 43 15 0.00% 44 17 0.00% 45 28 0.00% 46 26 0.00% 47 44 0.00% 48 34 0.00% 49 37 0.00% 50 36 0.00% 51 51 0.00% 52 70 0.00% 53 61 0.00% 54 61 0.00% 55 60 0.00% 56 75 0.00% 57 66 0.00% 58 66 0.00% 59 99 0.00% 60 114 0.00% 61 106 0.00% 62 107 0.00% 63 162 0.00% 64 169 0.00% 65 172 0.00% 66 180 0.00% 67 173 0.00% 68 220 0.00% 69 207 0.00% 70 242 0.00% 71 216 0.00% 72 273 0.00% 73 350 0.00% 74 355 0.00% 75 415 0.00% 76 372 0.00% 77 422 0.00% 78 477 0.00% 79 496 0.00% 80 540 0.00% 81 594 0.00% 82 677 0.00% 83 664 0.00% 84 746 0.00% 85 906 0.00% 86 899 0.00% 87 997 0.00% 88 983 0.00% 89 1144 0.00% 90 1188 0.00% 91 1204 0.00% 92 1284 0.00% 93 1417 0.00% 94 1364 0.00% 95 1592 0.00% 96 1593 0.00% 97 1707 0.00% 98 1857 0.00% 99 2020 0.00% 100 2152 0.00% 101 2191 0.00% 102 2292 0.00% 103 2379 0.00% 104 2665 0.01% 105 2729 0.01% 106 2874 0.01% 107 3052 0.01% 108 3290 0.01% 109 3212 0.01% 110 3480 0.01% 111 3750 0.01% 112 4006 0.01% 113 4043 0.01% 114 4262 0.01% 115 4504 0.01% 116 4914 0.01% 117 5063 0.01% 118 5154 0.01% 119 5435 0.01% 120 5979 0.01% 121 6238 0.01% 122 6713 0.01% 123 6819 0.01% 124 7100 0.01% 125 8106 0.02% 126 7377 0.01% 127 7284 0.01% 128 7815 0.01% 129 8615 0.02% 130 9090 0.02% 131 9661 0.02% 132 10197 0.02% 133 9716 0.02% 134 10333 0.02% 135 10117 0.02% 136 10883 0.02% 137 11106 0.02% 138 11742 0.02% 139 12336 0.02% 140 12562 0.02% 141 13856 0.03% 142 14206 0.03% 143 15034 0.03% 144 15176 0.03% 145 16567 0.03% 146 19015 0.04% 147 18063 0.03% 148 18966 0.04% 149 19893 0.04% 150 52522548 99.17% 52964383 reads passed initial QC criterion=sequence-density sequence-density=0.28 sequence-density-rank=1 fanout-score=0.00 fanout-score-rank=35 prefix-density=0.00 prefix-fanout=1.0 sequence=TGCGGGAACTTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=73.01 fanout-score-rank=1 prefix-density=0.18 prefix-fanout=2.5 sequence=TTTTTTCTTATACTTCTTATACTAAAGAACTAAACATTTGAATTCACCATTATTCCATGACTCCGATTTCAA criterion=sequence-density sequence-density=0.20 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=30 prefix-density=0.19 prefix-fanout=2.0 sequence=GTTGAACAAGTA criterion=fanout-score sequence-density=0.09 sequence-density-rank=20 fanout-score=116.11 fanout-score-rank=1 prefix-density=10.03 prefix-fanout=1.0 sequence=CTGGATAACTATCACTGAAAATC ERR11006607 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 19:47:15 Started mapping on | Dec 06 19:47:15 Finished on | Dec 06 19:55:14 Mapping speed, Million of reads per hour | 398.06 Number of input reads | 52964383 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 40837810 Uniquely mapped reads % | 77.10% Average mapped length | 298.47 Number of splices: Total | 13934718 Number of splices: Annotated (sjdb) | 12976217 Number of splices: GT/AG | 13575699 Number of splices: GC/AG | 154958 Number of splices: AT/AC | 25907 Number of splices: Non-canonical | 178154 Mismatch rate per base, % | 0.29% Deletion rate per base | 0.01% Deletion average length | 1.72 Insertion rate per base | 0.01% Insertion average length | 2.13 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 10212200 % of reads mapped to multiple loci | 19.28% Number of reads mapped to too many loci | 17136 % of reads mapped to too many loci | 0.03% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.10% % of reads unmapped: other | 0.48% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1914373 1914373 1914373 N_multimapping 10212200 10212200 10212200 N_noFeature 10578180 37820141 12602697 N_ambiguous 1639610 39536 648658 UnstrandedReadsAssigned:28620020 PositiveStrandReadsAssigned:2978133 NegativeStrandReadsAssigned:27586455 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006607 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006607-trimmed-pair1.fastq ERR11006607-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 52,964,383 reads, 30,015,503 reads pseudoaligned [quant] estimated average fragment length: 276.65 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,238 rounds 52973 ERR11006607.ke.tsv 35125 ERR11006607.se.tsv 88098 total ==> ERR11006607.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 660.776 0 0 PNS24247 1044 768.35 18.5428 0.745223 PNS24249 1928 1652.35 177.144 3.31051 PNS24246 1044 768.35 18.5428 0.745223 PNS24248 1044 768.35 18.5428 0.745223 PNS24244 1471 1195.35 20.2276 0.522541 PNS24243 293 51.6292 0 0 KQK14069 1603 1327.35 2104.22 48.9528 KQK14071 474 200.834 31.4383 4.83383 ==> ERR11006607.se.tsv <== BRADI_1g14170v3 2384 BRADI_1g53295v3 1590 BRADI_1g59795v3 278 BRADI_1g07683v3 0 BRADI_1g00485v3 4 BRADI_1g20270v3 128 BRADI_1g74790v3 302 BRADI_1g09890v3 0 BRADI_1g77505v3 103 BRADI_1g48960v3 0 ERR11006607 completed mapping pipeline successfully