Starting /dee2/code/volunteer_pipeline.sh ERR11006608 current disk space = 1549650141184 free memory = 1598459292 ERR11006608 SRAfilesize 1fc939423aed43cac859f87130626018 ERR11006608.sra ERR11006608.sra file validated ERR11006608 is paired end ERR11006608 is conventional basespace ERR11006608 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006608_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.24 37.0 37.0 37.0 37.0 37.0 2 36.0965 37.0 37.0 37.0 37.0 37.0 3 36.2935 37.0 37.0 37.0 37.0 37.0 4 36.423 37.0 37.0 37.0 37.0 37.0 5 36.3135 37.0 37.0 37.0 37.0 37.0 6 36.4035 37.0 37.0 37.0 37.0 37.0 7 36.264 37.0 37.0 37.0 37.0 37.0 8 36.4115 37.0 37.0 37.0 37.0 37.0 9 36.386 37.0 37.0 37.0 37.0 37.0 10-14 36.4486 37.0 37.0 37.0 37.0 37.0 15-19 36.430099999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.3683 37.0 37.0 37.0 37.0 37.0 25-29 36.348699999999994 37.0 37.0 37.0 37.0 37.0 30-34 36.3198 37.0 37.0 37.0 37.0 37.0 35-39 36.2269 37.0 37.0 37.0 37.0 37.0 40-44 36.1881 37.0 37.0 37.0 37.0 37.0 45-49 36.2249 37.0 37.0 37.0 37.0 37.0 50-54 36.216300000000004 37.0 37.0 37.0 37.0 37.0 55-59 36.2182 37.0 37.0 37.0 37.0 37.0 60-64 36.142500000000005 37.0 37.0 37.0 37.0 37.0 65-69 36.123000000000005 37.0 37.0 37.0 37.0 37.0 70-74 36.0962 37.0 37.0 37.0 37.0 37.0 75-79 36.0022 37.0 37.0 37.0 37.0 37.0 80-84 36.061699999999995 37.0 37.0 37.0 37.0 37.0 85-89 36.0178 37.0 37.0 37.0 37.0 37.0 90-94 35.9034 37.0 37.0 37.0 37.0 37.0 95-99 35.968 37.0 37.0 37.0 37.0 37.0 100-104 35.9522 37.0 37.0 37.0 37.0 37.0 105-109 35.867 37.0 37.0 37.0 37.0 37.0 110-114 35.7645 37.0 37.0 37.0 37.0 37.0 115-119 35.7411 37.0 37.0 37.0 37.0 37.0 120-124 35.7629 37.0 37.0 37.0 37.0 37.0 125-129 35.6322 37.0 37.0 37.0 37.0 37.0 130-134 35.549099999999996 37.0 37.0 37.0 37.0 37.0 135-139 35.509100000000004 37.0 37.0 37.0 37.0 37.0 140-144 35.3703 37.0 37.0 37.0 34.6 37.0 145-149 35.476 37.0 37.0 37.0 37.0 37.0 150 35.4195 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 2.0 23 2.0 24 2.0 25 2.0 26 3.0 27 12.0 28 26.0 29 37.0 30 35.0 31 70.0 32 89.0 33 107.0 34 165.0 35 327.0 36 2768.0 37 352.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.574999999999996 12.174999999999999 15.6 34.65 2 28.999999999999996 10.4 26.174999999999997 34.425 3 23.3 12.875 27.224999999999998 36.6 4 28.449999999999996 15.299999999999999 25.05 31.2 5 27.700000000000003 19.900000000000002 26.375 26.025 6 24.875 28.15 21.625 25.35 7 17.224999999999998 30.049999999999997 34.4 18.325 8 17.599999999999998 29.299999999999997 34.5 18.6 9 18.0 27.250000000000004 35.949999999999996 18.8 10-14 20.119999999999997 32.22 27.04 20.62 15-19 19.665 34.050000000000004 24.775 21.51 20-24 19.245 28.810000000000002 28.005000000000003 23.94 25-29 22.485 32.405 24.545 20.565 30-34 23.28 31.209999999999997 24.495 21.015 35-39 22.384999999999998 31.085 24.895 21.634999999999998 40-44 19.805 30.990000000000002 24.709999999999997 24.495 45-49 19.865 30.620000000000005 26.575 22.939999999999998 50-54 20.595 33.06 25.365 20.979999999999997 55-59 21.66 30.014999999999997 23.369999999999997 24.955 60-64 20.919999999999998 32.11 24.355 22.615 65-69 21.055 29.360000000000003 24.62 24.965 70-74 23.615 30.525000000000002 21.08 24.779999999999998 75-79 22.955000000000002 30.209999999999997 24.22 22.615 80-84 22.48 29.415000000000003 25.290000000000003 22.814999999999998 85-89 23.855 29.535 24.29 22.32 90-94 21.7 29.375 26.44 22.485 95-99 25.155 28.68 22.805 23.36 100-104 22.03 30.415 24.375 23.18 105-109 21.9 28.105000000000004 26.484999999999996 23.51 110-114 24.075 27.725 25.11 23.09 115-119 19.915 31.05 25.965 23.07 120-124 19.965 30.915 24.38 24.740000000000002 125-129 20.435 32.86 21.32 25.385 130-134 23.585 30.615 24.05 21.75 135-139 24.240000000000002 29.104999999999997 22.645 24.01 140-144 24.41 29.349999999999998 25.895000000000003 20.345 145-149 23.635 29.630000000000003 24.295 22.439999999999998 150 23.075000000000003 26.775 26.8 23.35 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 0.0 22 1.0 23 1.5 24 2.5 25 3.5 26 7.0 27 13.0 28 12.5 29 17.0 30 21.0 31 23.0 32 29.5 33 31.5 34 38.0 35 45.5 36 73.5 37 166.5 38 298.0 39 282.0 40 231.0 41 274.5 42 284.0 43 247.0 44 212.0 45 233.5 46 205.5 47 148.5 48 130.0 49 97.5 50 67.5 51 58.0 52 48.5 53 36.0 54 28.0 55 21.5 56 25.5 57 23.0 58 23.5 59 27.5 60 29.0 61 29.5 62 34.0 63 36.0 64 56.0 65 74.5 66 46.0 67 25.0 68 24.0 69 19.0 70 17.5 71 20.5 72 18.0 73 14.5 74 11.0 75 9.5 76 9.0 77 6.5 78 7.5 79 7.0 80 4.5 81 3.0 82 2.0 83 3.0 84 2.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.650000000000006 #Duplication Level Percentage of deduplicated Percentage of total 1 79.11597729115978 48.775 2 12.73317112733171 15.7 3 3.2035685320356855 5.925 4 1.5815085158150852 3.9 5 0.851581508515815 2.625 6 0.7299270072992701 2.7 7 0.24330900243309003 1.05 8 0.28386050283860503 1.4000000000000001 9 0.04055150040551501 0.22499999999999998 >10 1.1759935117599352 14.149999999999999 >50 0.0 0.0 >100 0.04055150040551501 3.55 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 142 3.55 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 45 1.125 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 40 1.0 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 35 0.8750000000000001 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 35 0.8750000000000001 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 30 0.75 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 30 0.75 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 28 0.7000000000000001 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 24 0.6 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 22 0.5499999999999999 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 21 0.525 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 19 0.475 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 19 0.475 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 18 0.44999999999999996 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 17 0.42500000000000004 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 16 0.4 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 15 0.375 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 14 0.35000000000000003 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 14 0.35000000000000003 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 13 0.325 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 13 0.325 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 12 0.3 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 12 0.3 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 12 0.3 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 11 0.27499999999999997 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 11 0.27499999999999997 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 10 0.25 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 10 0.25 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 10 0.25 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 10 0.25 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 9 0.22499999999999998 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 8 0.2 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 8 0.2 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 8 0.2 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 8 0.2 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 8 0.2 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 8 0.2 No Hit CACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCG 8 0.2 No Hit TTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGC 7 0.17500000000000002 No Hit TCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCG 7 0.17500000000000002 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 7 0.17500000000000002 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 7 0.17500000000000002 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 7 0.17500000000000002 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 7 0.17500000000000002 No Hit TCTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTA 6 0.15 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 6 0.15 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 6 0.15 No Hit GCCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 6 0.15 No Hit ACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 6 0.15 No Hit TGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGA 6 0.15 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 6 0.15 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 6 0.15 No Hit TGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAG 6 0.15 No Hit GGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAG 6 0.15 No Hit TCCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 6 0.15 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 6 0.15 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 6 0.15 No Hit GAGCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTA 6 0.15 No Hit CACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCA 6 0.15 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 6 0.15 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 6 0.15 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 6 0.15 No Hit CGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATA 5 0.125 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 5 0.125 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 5 0.125 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 5 0.125 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 5 0.125 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 5 0.125 No Hit GCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCC 5 0.125 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 5 0.125 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 5 0.125 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 5 0.125 No Hit TGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCT 5 0.125 No Hit CGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAAC 5 0.125 No Hit CCACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAA 5 0.125 No Hit CAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGA 5 0.125 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 5 0.125 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 5 0.125 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 5 0.125 No Hit AGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCT 5 0.125 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 5 0.125 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 5 0.125 No Hit AGGGCTATAGTCATAGTGATCCTCCTATTCAATTACTTCAACCATTTCCG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0125 0.0 0.0 0.0 0.0 102-103 0.025 0.0 0.0 0.0 0.0 104-105 0.025 0.0 0.0 0.0 0.0 106-107 0.05 0.0 0.0 0.0 0.0 108-109 0.075 0.0 0.0 0.0 0.0 110-111 0.075 0.0 0.0 0.0 0.0 112-113 0.1 0.0 0.0 0.0 0.0 114-115 0.1 0.0 0.0 0.0 0.0 116-117 0.1 0.0 0.0 0.0 0.0 118-119 0.1 0.0 0.0 0.0 0.0 120-121 0.1 0.0 0.0 0.0 0.0 122-123 0.1375 0.0 0.0 0.0 0.0 124-125 0.15 0.0 0.0 0.0 0.0 126-127 0.175 0.0 0.0 0.0 0.0 128-129 0.1875 0.0 0.0 0.0 0.0 130-131 0.2375 0.0 0.0 0.0 0.0 132-133 0.275 0.0 0.0 0.0 0.0 134-135 0.3 0.0 0.0 0.0 0.0 136-137 0.325 0.0 0.0 0.0 0.0 138 0.325 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCCTCA 10 0.006973645 144.0 1 TAGCAGA 40 0.005777437 54.0 9 CATTAGC 40 0.005777437 54.0 6 CCTCATT 40 0.005777437 54.0 3 CTCATTA 40 0.005777437 54.0 4 CCCTCAT 40 0.005777437 54.0 2 TTAGCAG 40 0.005777437 54.0 8 TCATTAG 45 0.009205684 48.0 5 TATGTTA 20 0.006139246 28.8 25-29 CGATAAT 35 0.0036813593 20.571428 110-114 GCGATGA 35 0.0036813593 20.571428 100-104 GCGATAA 35 0.0036813593 20.571428 110-114 ATCTACT 35 0.0036813593 20.571428 85-89 TGCGATG 35 0.0036813593 20.571428 100-104 GCGGTCA 35 0.0036813593 20.571428 130-134 TCAATAT 35 0.0036813593 20.571428 80-84 CAATATC 35 0.0036813593 20.571428 80-84 GCTCGCG 35 0.0036813593 20.571428 65-69 CTGGAGG 35 0.0036813593 20.571428 90-94 GGCTCGC 35 0.0036813593 20.571428 65-69 >>END_MODULE ERR11006608 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006608_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.5365 37.0 37.0 37.0 37.0 37.0 2 36.039 37.0 37.0 37.0 37.0 37.0 3 36.3165 37.0 37.0 37.0 37.0 37.0 4 36.2595 37.0 37.0 37.0 37.0 37.0 5 36.272 37.0 37.0 37.0 37.0 37.0 6 36.2445 37.0 37.0 37.0 37.0 37.0 7 36.227 37.0 37.0 37.0 37.0 37.0 8 36.255 37.0 37.0 37.0 37.0 37.0 9 36.2585 37.0 37.0 37.0 37.0 37.0 10-14 36.2587 37.0 37.0 37.0 37.0 37.0 15-19 36.2346 37.0 37.0 37.0 37.0 37.0 20-24 36.195899999999995 37.0 37.0 37.0 37.0 37.0 25-29 36.1887 37.0 37.0 37.0 37.0 37.0 30-34 36.1703 37.0 37.0 37.0 37.0 37.0 35-39 36.093399999999995 37.0 37.0 37.0 37.0 37.0 40-44 36.1189 37.0 37.0 37.0 37.0 37.0 45-49 36.074 37.0 37.0 37.0 37.0 37.0 50-54 35.9883 37.0 37.0 37.0 37.0 37.0 55-59 35.9781 37.0 37.0 37.0 37.0 37.0 60-64 35.9194 37.0 37.0 37.0 37.0 37.0 65-69 35.876099999999994 37.0 37.0 37.0 37.0 37.0 70-74 35.852999999999994 37.0 37.0 37.0 37.0 37.0 75-79 35.8506 37.0 37.0 37.0 37.0 37.0 80-84 35.7703 37.0 37.0 37.0 37.0 37.0 85-89 35.7079 37.0 37.0 37.0 37.0 37.0 90-94 35.7354 37.0 37.0 37.0 37.0 37.0 95-99 35.521699999999996 37.0 37.0 37.0 37.0 37.0 100-104 35.574600000000004 37.0 37.0 37.0 37.0 37.0 105-109 35.4184 37.0 37.0 37.0 37.0 37.0 110-114 35.3738 37.0 37.0 37.0 37.0 37.0 115-119 35.332899999999995 37.0 37.0 37.0 34.6 37.0 120-124 35.1337 37.0 37.0 37.0 27.4 37.0 125-129 35.198499999999996 37.0 37.0 37.0 27.4 37.0 130-134 35.0795 37.0 37.0 37.0 25.0 37.0 135-139 35.0146 37.0 37.0 37.0 25.0 37.0 140-144 34.947 37.0 37.0 37.0 25.0 37.0 145-149 35.0284 37.0 37.0 37.0 25.0 37.0 150 34.552 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 1.0 18 1.0 19 2.0 20 1.0 21 1.0 22 3.0 23 2.0 24 5.0 25 13.0 26 15.0 27 16.0 28 23.0 29 35.0 30 50.0 31 61.0 32 78.0 33 116.0 34 214.0 35 614.0 36 2585.0 37 163.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.534966819806023 24.527820316488004 19.372128637059724 27.565084226646245 2 25.650000000000002 25.025 33.275 16.05 3 21.425 27.55 33.625 17.4 4 24.224999999999998 27.750000000000004 26.75 21.275 5 23.674999999999997 28.7 29.599999999999998 18.025 6 20.349999999999998 32.875 27.700000000000003 19.075 7 20.9 20.200000000000003 41.05 17.849999999999998 8 21.575 23.3 31.3 23.825 9 23.175 20.05 35.075 21.7 10-14 23.71 25.865 29.630000000000003 20.794999999999998 15-19 24.13 24.95 30.330000000000002 20.59 20-24 24.185000000000002 23.97 31.1 20.745 25-29 24.215 24.03 30.3 21.455 30-34 24.72 23.77 30.31 21.2 35-39 24.445 24.445 29.770000000000003 21.34 40-44 22.945 25.380000000000003 29.815 21.86 45-49 22.505 26.815 29.5 21.18 50-54 22.795 26.27 28.799999999999997 22.134999999999998 55-59 22.869999999999997 24.740000000000002 29.075 23.315 60-64 22.575 25.119999999999997 29.73 22.575 65-69 23.385 24.965 29.465000000000003 22.185 70-74 23.580000000000002 25.09 29.654999999999998 21.675 75-79 23.57 24.755 30.885 20.79 80-84 24.6 24.815 29.330000000000002 21.255 85-89 25.345000000000002 25.314999999999998 27.200000000000003 22.14 90-94 23.51 25.05 28.749999999999996 22.689999999999998 95-99 23.52 24.88 29.235 22.365 100-104 25.130000000000003 23.09 30.29 21.490000000000002 105-109 25.564999999999998 22.605 29.970000000000002 21.86 110-114 24.115000000000002 23.369999999999997 29.955 22.56 115-119 24.085 24.745 29.104999999999997 22.065 120-124 24.075 24.875 28.575 22.475 125-129 22.35 25.759999999999998 29.995 21.895 130-134 22.96 26.205000000000002 27.994999999999997 22.84 135-139 23.59 25.41 29.835 21.165 140-144 23.455000000000002 25.080000000000002 29.945 21.52 145-149 22.665 24.335 31.005 21.995 150 23.150000000000002 23.200000000000003 30.475 23.175 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 1.5 21 3.0 22 2.0 23 3.0 24 9.5 25 17.0 26 16.0 27 14.5 28 15.0 29 22.5 30 31.0 31 27.0 32 33.5 33 40.0 34 54.5 35 84.0 36 132.0 37 210.0 38 231.5 39 210.5 40 231.5 41 255.0 42 226.0 43 222.5 44 228.5 45 193.0 46 178.0 47 136.0 48 101.0 49 89.5 50 68.5 51 57.5 52 47.0 53 37.0 54 35.0 55 33.0 56 32.5 57 33.0 58 24.5 59 29.0 60 33.5 61 27.5 62 30.0 63 56.0 64 75.5 65 60.5 66 42.0 67 31.0 68 30.0 69 27.5 70 20.0 71 21.0 72 25.0 73 22.5 74 14.5 75 14.5 76 14.5 77 9.0 78 8.5 79 7.0 80 3.5 81 3.0 82 1.0 83 0.0 84 1.0 85 1.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.0500000000000003 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.97500000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 78.03001154290111 50.7 2 13.543670642554828 17.599999999999998 3 3.770681031165833 7.35 4 1.1542901115813775 3.0 5 1.0388611004232395 3.375 6 0.461716044632551 1.7999999999999998 7 0.42323970757983836 1.925 8 0.2308580223162755 1.2 9 0.2693343593689881 1.575 >10 1.0388611004232395 9.975000000000001 >50 0.038476337052712584 1.5 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 60 1.5 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 29 0.7250000000000001 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 26 0.65 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 23 0.575 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 22 0.5499999999999999 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 22 0.5499999999999999 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 20 0.5 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 18 0.44999999999999996 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 16 0.4 No Hit TATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACC 16 0.4 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 15 0.375 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 14 0.35000000000000003 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 13 0.325 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 13 0.325 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 13 0.325 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 12 0.3 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 12 0.3 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 12 0.3 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 11 0.27499999999999997 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 11 0.27499999999999997 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 11 0.27499999999999997 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 10 0.25 No Hit CTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTC 10 0.25 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 10 0.25 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 10 0.25 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 10 0.25 No Hit CTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGG 10 0.25 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 10 0.25 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 9 0.22499999999999998 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 9 0.22499999999999998 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 9 0.22499999999999998 No Hit CTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATT 9 0.22499999999999998 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 9 0.22499999999999998 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 9 0.22499999999999998 No Hit CTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTC 9 0.22499999999999998 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 8 0.2 No Hit GCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGC 8 0.2 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 8 0.2 No Hit TTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGG 8 0.2 No Hit GAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCG 8 0.2 No Hit TCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGA 8 0.2 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 7 0.17500000000000002 No Hit GGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAA 7 0.17500000000000002 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 7 0.17500000000000002 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 7 0.17500000000000002 No Hit GGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTA 7 0.17500000000000002 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 7 0.17500000000000002 No Hit GTATAAACGAATTTTTTTTATTTTCTTAGACTTAGACCCTGCAAGATAAT 7 0.17500000000000002 No Hit GAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTA 7 0.17500000000000002 No Hit AACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGG 7 0.17500000000000002 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 7 0.17500000000000002 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 7 0.17500000000000002 No Hit TGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGA 6 0.15 No Hit AGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAA 6 0.15 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 6 0.15 No Hit GCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGT 6 0.15 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 6 0.15 No Hit GTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCAT 6 0.15 No Hit GCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGC 6 0.15 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 6 0.15 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 6 0.15 No Hit GTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAAT 6 0.15 No Hit TCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTG 6 0.15 No Hit GTGGACGTTGCCGTAGCGCTGCGGGCCTGGTCTGGGTGTGCTACTGATGG 6 0.15 No Hit AGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTT 5 0.125 No Hit GGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTC 5 0.125 No Hit CTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCG 5 0.125 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 5 0.125 No Hit GGTATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATC 5 0.125 No Hit GGACGTTGCCGTAGCGCTGCGGGCCTGGTCTGGGTGTGCTACTGATGGCC 5 0.125 No Hit ATATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCAC 5 0.125 No Hit AACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAG 5 0.125 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 5 0.125 No Hit TGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGC 5 0.125 No Hit GGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGGCCTGGTCT 5 0.125 No Hit CTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAG 5 0.125 No Hit TCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTG 5 0.125 No Hit AGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCT 5 0.125 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 5 0.125 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 5 0.125 No Hit TAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAG 5 0.125 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 5 0.125 No Hit AGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACT 5 0.125 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 5 0.125 No Hit TTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGA 5 0.125 No Hit GCTGCATCCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAAT 5 0.125 No Hit AATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTA 5 0.125 No Hit ATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAG 5 0.125 No Hit TGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAA 5 0.125 No Hit GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTAT 5 0.125 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0125 0.0 0.0 0.0 0.0 102-103 0.025 0.0 0.0 0.0 0.0 104-105 0.025 0.0 0.0 0.0 0.0 106-107 0.05 0.0 0.0 0.0 0.0 108-109 0.075 0.0 0.0 0.0 0.0 110-111 0.075 0.0 0.0 0.0 0.0 112-113 0.1 0.0 0.0 0.0 0.0 114-115 0.1 0.0 0.0 0.0 0.0 116-117 0.1 0.0 0.0 0.0 0.0 118-119 0.1 0.0 0.0 0.0 0.0 120-121 0.1 0.0 0.0 0.0 0.0 122-123 0.1375 0.0 0.0 0.0 0.0 124-125 0.15 0.0 0.0 0.0 0.0 126-127 0.175 0.0 0.0 0.0 0.0 128-129 0.1875 0.0 0.0 0.0 0.0 130-131 0.2625 0.0 0.0 0.0 0.0 132-133 0.3 0.0 0.0 0.0 0.0 134-135 0.325 0.0 0.0 0.0 0.0 136-137 0.35 0.0 0.0 0.0 0.0 138 0.35 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 2495258 spots for ERR11006608.sra Written 2495258 spots for ERR11006608.sra Read 2495258 spots for ERR11006608.sra Written 2495258 spots for ERR11006608.sra Read 2495258 spots for ERR11006608.sra Written 2495258 spots for ERR11006608.sra Read 2495258 spots for ERR11006608.sra Written 2495258 spots for ERR11006608.sra Read 2495258 spots for ERR11006608.sra Written 2495258 spots for ERR11006608.sra Read 2495258 spots for ERR11006608.sra Written 2495258 spots for ERR11006608.sra Read 2495258 spots for ERR11006608.sra Written 2495258 spots for ERR11006608.sra Read 2495258 spots for ERR11006608.sra Written 2495258 spots for ERR11006608.sra Read 2495263 spots for ERR11006608.sra Written 2495263 spots for ERR11006608.sra Read 2495258 spots for ERR11006608.sra Written 2495258 spots for ERR11006608.sra Read 2495258 spots for ERR11006608.sra Written 2495258 spots for ERR11006608.sra Read 2495258 spots for ERR11006608.sra Written 2495258 spots for ERR11006608.sra Read 2495258 spots for ERR11006608.sra Written 2495258 spots for ERR11006608.sra Read 2495258 spots for ERR11006608.sra Written 2495258 spots for ERR11006608.sra Read 2495258 spots for ERR11006608.sra Written 2495258 spots for ERR11006608.sra Read 2495258 spots for ERR11006608.sra Written 2495258 spots for ERR11006608.sra Read 2495258 spots for ERR11006608.sra Written 2495258 spots for ERR11006608.sra Read 2495258 spots for ERR11006608.sra Written 2495258 spots for ERR11006608.sra Read 2495258 spots for ERR11006608.sra Written 2495258 spots for ERR11006608.sra Read 2495258 spots for ERR11006608.sra Written 2495258 spots for ERR11006608.sra SRR ids: ['ERR11006608.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_ujxh3i7u ERR11006608.sra spots: 49905165 blocks: [[1, 2495258], [2495259, 4990516], [4990517, 7485774], [7485775, 9981032], [9981033, 12476290], [12476291, 14971548], [14971549, 17466806], [17466807, 19962064], [19962065, 22457322], [22457323, 24952580], [24952581, 27447838], [27447839, 29943096], [29943097, 32438354], [32438355, 34933612], [34933613, 37428870], [37428871, 39924128], [39924129, 42419386], [42419387, 44914644], [44914645, 47409902], [47409903, 49905165]] ERR11006608 file size 18336396 ERR11006608 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006608 ERR11006608_1.fastq ERR11006608_2.fastq Input file: ERR11006608_1.fastq Paired file: ERR11006608_2.fastq trimmed: ERR11006608-trimmed-pair1.fastq, ERR11006608-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 19:46:37 2024 >> started Fri Dec 6 19:47:42 2024 >> done (65.292s) 49905165 read pairs processed; of these: 141 ( 0.00%) short read pairs filtered out after trimming by size control 969 ( 0.00%) empty read pairs filtered out after trimming by size control 49904055 (100.00%) read pairs available; of these: 300056 ( 0.60%) trimmed read pairs available after processing 49603999 (99.40%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 7 0.00% 19 4 0.00% 20 6 0.00% 21 2 0.00% 22 18 0.00% 23 5 0.00% 24 9 0.00% 25 22 0.00% 26 10 0.00% 27 14 0.00% 28 19 0.00% 29 15 0.00% 30 15 0.00% 31 37 0.00% 32 11 0.00% 33 19 0.00% 34 15 0.00% 35 20 0.00% 36 16 0.00% 37 16 0.00% 38 12 0.00% 39 19 0.00% 40 25 0.00% 41 23 0.00% 42 17 0.00% 43 26 0.00% 44 30 0.00% 45 28 0.00% 46 23 0.00% 47 28 0.00% 48 31 0.00% 49 38 0.00% 50 37 0.00% 51 37 0.00% 52 60 0.00% 53 51 0.00% 54 61 0.00% 55 55 0.00% 56 57 0.00% 57 68 0.00% 58 71 0.00% 59 69 0.00% 60 89 0.00% 61 99 0.00% 62 102 0.00% 63 131 0.00% 64 128 0.00% 65 131 0.00% 66 168 0.00% 67 171 0.00% 68 168 0.00% 69 158 0.00% 70 174 0.00% 71 204 0.00% 72 219 0.00% 73 245 0.00% 74 261 0.00% 75 293 0.00% 76 302 0.00% 77 332 0.00% 78 362 0.00% 79 365 0.00% 80 363 0.00% 81 445 0.00% 82 450 0.00% 83 512 0.00% 84 525 0.00% 85 623 0.00% 86 633 0.00% 87 664 0.00% 88 734 0.00% 89 742 0.00% 90 804 0.00% 91 848 0.00% 92 960 0.00% 93 953 0.00% 94 994 0.00% 95 1108 0.00% 96 1121 0.00% 97 1208 0.00% 98 1299 0.00% 99 1391 0.00% 100 1445 0.00% 101 1501 0.00% 102 1534 0.00% 103 1661 0.00% 104 1729 0.00% 105 1814 0.00% 106 1943 0.00% 107 2100 0.00% 108 2161 0.00% 109 2188 0.00% 110 2414 0.00% 111 2457 0.00% 112 2814 0.01% 113 2743 0.01% 114 2812 0.01% 115 3058 0.01% 116 3239 0.01% 117 3367 0.01% 118 3492 0.01% 119 3655 0.01% 120 4014 0.01% 121 4250 0.01% 122 4584 0.01% 123 4726 0.01% 124 4754 0.01% 125 5642 0.01% 126 4979 0.01% 127 4884 0.01% 128 5349 0.01% 129 5772 0.01% 130 6224 0.01% 131 6531 0.01% 132 6992 0.01% 133 6600 0.01% 134 6933 0.01% 135 6715 0.01% 136 7244 0.01% 137 7306 0.01% 138 7839 0.02% 139 8178 0.02% 140 8473 0.02% 141 9056 0.02% 142 9471 0.02% 143 10100 0.02% 144 10108 0.02% 145 11326 0.02% 146 13450 0.03% 147 12525 0.03% 148 12670 0.03% 149 13609 0.03% 150 49603999 99.40% 49904055 reads passed initial QC criterion=sequence-density sequence-density=0.32 sequence-density-rank=1 fanout-score=0.00 fanout-score-rank=35 prefix-density=0.00 prefix-fanout=1.0 sequence=TGCGGGAACTTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=51.85 fanout-score-rank=1 prefix-density=0.15 prefix-fanout=2.3 sequence=TTTTTTCTTATACTTCTTATACTAAAGAACTAAACATTTGAATTCACCATTATTCCATGACTCCGATTTCAA criterion=sequence-density sequence-density=0.31 sequence-density-rank=1 fanout-score=11.10 fanout-score-rank=7 prefix-density=3.42 prefix-fanout=1.0 sequence=TTGCGTAGTGGATCTGCTGGGGCCTATGCGAAAGCTGGGCCTCACGAATTCTATAGTGGCAGGCACCGCGTTAGGCTGGCTTC criterion=fanout-score sequence-density=0.12 sequence-density-rank=10 fanout-score=94.64 fanout-score-rank=1 prefix-density=11.65 prefix-fanout=1.0 sequence=CTGGATAACTATCACTGAAAATC ERR11006608 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 19:48:20 Started mapping on | Dec 06 19:48:21 Finished on | Dec 06 19:54:09 Mapping speed, Million of reads per hour | 516.25 Number of input reads | 49904055 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 39248524 Uniquely mapped reads % | 78.65% Average mapped length | 298.52 Number of splices: Total | 12370536 Number of splices: Annotated (sjdb) | 11492112 Number of splices: GT/AG | 12046386 Number of splices: GC/AG | 139069 Number of splices: AT/AC | 19522 Number of splices: Non-canonical | 165559 Mismatch rate per base, % | 0.30% Deletion rate per base | 0.01% Deletion average length | 1.64 Insertion rate per base | 0.01% Insertion average length | 2.17 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 8964554 % of reads mapped to multiple loci | 17.96% Number of reads mapped to too many loci | 9563 % of reads mapped to too many loci | 0.02% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.06% % of reads unmapped: other | 0.31% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1690977 1690977 1690977 N_multimapping 8964554 8964554 8964554 N_noFeature 9966440 36460822 11846156 N_ambiguous 1443384 42671 526658 UnstrandedReadsAssigned:27838700 PositiveStrandReadsAssigned:2745031 NegativeStrandReadsAssigned:26875710 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006608 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006608-trimmed-pair1.fastq ERR11006608-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 49,904,055 reads, 28,022,679 reads pseudoaligned [quant] estimated average fragment length: 279.078 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,151 rounds 52973 ERR11006608.ke.tsv 35125 ERR11006608.se.tsv 88098 total ==> ERR11006608.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 658.295 0 0 PNS24247 1044 765.922 11.168 0.462414 PNS24249 1928 1649.92 91.5575 1.75984 PNS24246 1044 765.922 11.168 0.462414 PNS24248 1044 765.922 11.168 0.462414 PNS24244 1471 1192.92 19.9386 0.530059 PNS24243 293 49.8053 0 0 KQK14069 1603 1324.92 1959.12 46.8935 KQK14071 474 198.941 34.5322 5.5048 ==> ERR11006608.se.tsv <== BRADI_1g14170v3 2216 BRADI_1g53295v3 934 BRADI_1g59795v3 299 BRADI_1g07683v3 1 BRADI_1g00485v3 1 BRADI_1g20270v3 139 BRADI_1g74790v3 250 BRADI_1g09890v3 0 BRADI_1g77505v3 122 BRADI_1g48960v3 2 ERR11006608 completed mapping pipeline successfully