Starting /dee2/code/volunteer_pipeline.sh ERR11006609 current disk space = 1549505544192 free memory = 1599991036 ERR11006609 SRAfilesize 7938ccdc1296da7dce7f05f0f8149e76 ERR11006609.sra ERR11006609.sra file validated ERR11006609 is paired end ERR11006609 is conventional basespace ERR11006609 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006609_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.251 37.0 37.0 37.0 37.0 37.0 2 36.1905 37.0 37.0 37.0 37.0 37.0 3 36.2435 37.0 37.0 37.0 37.0 37.0 4 36.2835 37.0 37.0 37.0 37.0 37.0 5 36.4255 37.0 37.0 37.0 37.0 37.0 6 36.3875 37.0 37.0 37.0 37.0 37.0 7 36.264 37.0 37.0 37.0 37.0 37.0 8 36.338 37.0 37.0 37.0 37.0 37.0 9 36.3815 37.0 37.0 37.0 37.0 37.0 10-14 36.400999999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.408500000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.331399999999995 37.0 37.0 37.0 37.0 37.0 25-29 36.265 37.0 37.0 37.0 37.0 37.0 30-34 36.241600000000005 37.0 37.0 37.0 37.0 37.0 35-39 36.2014 37.0 37.0 37.0 37.0 37.0 40-44 36.170899999999996 37.0 37.0 37.0 37.0 37.0 45-49 36.1528 37.0 37.0 37.0 37.0 37.0 50-54 36.1419 37.0 37.0 37.0 37.0 37.0 55-59 36.116200000000006 37.0 37.0 37.0 37.0 37.0 60-64 36.0886 37.0 37.0 37.0 37.0 37.0 65-69 36.0977 37.0 37.0 37.0 37.0 37.0 70-74 36.074400000000004 37.0 37.0 37.0 37.0 37.0 75-79 36.003 37.0 37.0 37.0 37.0 37.0 80-84 35.958 37.0 37.0 37.0 37.0 37.0 85-89 35.936699999999995 37.0 37.0 37.0 37.0 37.0 90-94 35.8524 37.0 37.0 37.0 37.0 37.0 95-99 35.9553 37.0 37.0 37.0 37.0 37.0 100-104 35.9363 37.0 37.0 37.0 37.0 37.0 105-109 35.8251 37.0 37.0 37.0 37.0 37.0 110-114 35.6848 37.0 37.0 37.0 37.0 37.0 115-119 35.7171 37.0 37.0 37.0 37.0 37.0 120-124 35.718599999999995 37.0 37.0 37.0 37.0 37.0 125-129 35.545899999999996 37.0 37.0 37.0 37.0 37.0 130-134 35.440400000000004 37.0 37.0 37.0 37.0 37.0 135-139 35.4187 37.0 37.0 37.0 37.0 37.0 140-144 35.213100000000004 37.0 37.0 37.0 27.4 37.0 145-149 35.3595 37.0 37.0 37.0 37.0 37.0 150 35.311 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 1.0 22 1.0 23 3.0 24 6.0 25 5.0 26 9.0 27 15.0 28 22.0 29 33.0 30 50.0 31 74.0 32 87.0 33 107.0 34 156.0 35 343.0 36 2769.0 37 318.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.65 13.125 15.024999999999999 34.2 2 29.349999999999998 10.674999999999999 26.450000000000003 33.525 3 23.375 13.175 27.224999999999998 36.225 4 27.625 14.799999999999999 26.224999999999998 31.35 5 27.35 20.05 26.25 26.35 6 22.85 30.075000000000003 20.95 26.125 7 16.425 30.75 35.25 17.575 8 16.6 29.45 34.050000000000004 19.900000000000002 9 17.4 29.125 34.225 19.25 10-14 19.68 32.82 27.025 20.474999999999998 15-19 19.79 35.394999999999996 23.995 20.82 20-24 19.24 28.955 28.634999999999998 23.169999999999998 25-29 22.525000000000002 32.029999999999994 24.98 20.465 30-34 23.56 31.915 24.145 20.380000000000003 35-39 22.665 32.09 24.63 20.615 40-44 19.525000000000002 31.924999999999997 25.34 23.21 45-49 20.1 30.705 26.915 22.28 50-54 20.880000000000003 32.485 25.490000000000002 21.145 55-59 22.125 30.04 23.799999999999997 24.035 60-64 20.560000000000002 31.8 24.91 22.73 65-69 21.27 29.9 24.91 23.919999999999998 70-74 22.855 30.385 22.37 24.39 75-79 22.759999999999998 30.19 25.374999999999996 21.675 80-84 22.405 30.085 24.595 22.915 85-89 24.18 29.92 24.435000000000002 21.465 90-94 21.29 29.93 26.384999999999998 22.395 95-99 24.12 30.035 23.57 22.275 100-104 21.66 30.320000000000004 24.57 23.45 105-109 22.16 28.615000000000002 26.040000000000003 23.185 110-114 23.24 28.115000000000002 25.16 23.485 115-119 19.29 31.580000000000002 26.005 23.125 120-124 19.335 30.53 25.169999999999998 24.965 125-129 20.485 31.855 21.81 25.85 130-134 22.52 30.675 24.84 21.965 135-139 23.919999999999998 29.659999999999997 22.875 23.544999999999998 140-144 24.245 29.64 25.924999999999997 20.19 145-149 22.665 30.669999999999998 24.925 21.740000000000002 150 22.8 27.85 26.174999999999997 23.175 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 1.5 22 1.5 23 2.0 24 3.0 25 1.5 26 1.5 27 8.0 28 12.5 29 14.5 30 23.0 31 28.0 32 28.0 33 33.5 34 41.5 35 58.0 36 93.5 37 184.0 38 306.5 39 291.0 40 248.5 41 293.5 42 283.0 43 257.5 44 230.5 45 215.0 46 207.5 47 164.0 48 113.0 49 82.0 50 73.0 51 46.0 52 30.5 53 32.5 54 31.0 55 31.5 56 22.0 57 14.5 58 20.0 59 27.5 60 29.5 61 26.5 62 22.5 63 25.0 64 53.0 65 70.0 66 42.0 67 20.5 68 19.0 69 15.0 70 17.5 71 18.5 72 13.0 73 13.5 74 11.5 75 6.5 76 8.0 77 9.0 78 7.0 79 4.0 80 2.0 81 2.0 82 1.5 83 1.5 84 2.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.199999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 78.23920265780731 47.099999999999994 2 13.82890365448505 16.650000000000002 3 2.990033222591362 5.4 4 1.1627906976744187 2.8000000000000003 5 0.9551495016611296 2.875 6 0.5398671096345514 1.95 7 0.29069767441860467 1.225 8 0.20764119601328906 1.0 9 0.16611295681063123 0.8999999999999999 >10 1.5780730897009967 16.55 >50 0.0 0.0 >100 0.04152823920265781 3.55 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 142 3.55 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 39 0.975 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 38 0.95 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 34 0.8500000000000001 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 33 0.8250000000000001 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 31 0.775 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 27 0.675 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 24 0.6 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 23 0.575 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 21 0.525 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 20 0.5 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 19 0.475 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 19 0.475 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 19 0.475 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 18 0.44999999999999996 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 18 0.44999999999999996 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 16 0.4 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 16 0.4 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 16 0.4 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 15 0.375 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 14 0.35000000000000003 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 14 0.35000000000000003 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 12 0.3 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 12 0.3 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 12 0.3 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 12 0.3 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 12 0.3 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 12 0.3 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 11 0.27499999999999997 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 11 0.27499999999999997 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 11 0.27499999999999997 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 11 0.27499999999999997 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 11 0.27499999999999997 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 11 0.27499999999999997 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 10 0.25 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 10 0.25 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 10 0.25 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 10 0.25 No Hit CACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAAT 10 0.25 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 9 0.22499999999999998 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 9 0.22499999999999998 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 9 0.22499999999999998 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 9 0.22499999999999998 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 8 0.2 No Hit ACCGCCGAAAACACCAGCTACACCTAACATGTGAAATGGATGCATAAGGA 8 0.2 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 8 0.2 No Hit CACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCG 8 0.2 No Hit TCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTT 8 0.2 No Hit TCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCG 7 0.17500000000000002 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 7 0.17500000000000002 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 7 0.17500000000000002 No Hit GCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTA 7 0.17500000000000002 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 7 0.17500000000000002 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 7 0.17500000000000002 No Hit TAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGA 7 0.17500000000000002 No Hit ACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGT 6 0.15 No Hit TTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATATC 6 0.15 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 6 0.15 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 6 0.15 No Hit GGATCATAATAAAATGGATTTTAGGTATCTAGGGAAAATTCACTTCGAAG 6 0.15 No Hit GCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCC 6 0.15 No Hit TCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGT 6 0.15 No Hit ATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTT 6 0.15 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 6 0.15 No Hit GAGCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTA 6 0.15 No Hit AGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGA 6 0.15 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 6 0.15 No Hit GCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTC 6 0.15 No Hit GGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAA 5 0.125 No Hit AGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAG 5 0.125 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 5 0.125 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 5 0.125 No Hit TGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATA 5 0.125 No Hit GCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGAT 5 0.125 No Hit CTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAG 5 0.125 No Hit TCCCACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAAC 5 0.125 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 5 0.125 No Hit GTTCGATCTATGGTCATTGAGGGCCTCCTAAAAAGATCTACTAAATTCAT 5 0.125 No Hit CCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAA 5 0.125 No Hit TGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAG 5 0.125 No Hit GGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAG 5 0.125 No Hit GTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCG 5 0.125 No Hit GGGGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAAC 5 0.125 No Hit GTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTA 5 0.125 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 5 0.125 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 5 0.125 No Hit GTCCATGTACCAGTAGAAGATTCGGCAGCTACTGCAGCCCCTGCTTCTTC 5 0.125 No Hit CGGTCGAACTACCAGAATGTCTAGAAATGTAGAGCTTAAACTAGAAAGGC 5 0.125 No Hit TCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAA 5 0.125 No Hit TAGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTT 5 0.125 No Hit AGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.05 0.0 0.0 0.0 0.0 92-93 0.05 0.0 0.0 0.0 0.0 94-95 0.05 0.0 0.0 0.0 0.0 96-97 0.05 0.0 0.0 0.0 0.0 98-99 0.05 0.0 0.0 0.0 0.0 100-101 0.05 0.0 0.0 0.0 0.0 102-103 0.05 0.0 0.0 0.0 0.0 104-105 0.05 0.0 0.0 0.0 0.0 106-107 0.0875 0.0 0.0 0.0 0.0 108-109 0.125 0.0 0.0 0.0 0.0 110-111 0.125 0.0 0.0 0.0 0.0 112-113 0.125 0.0 0.0 0.0 0.0 114-115 0.125 0.0 0.0 0.0 0.0 116-117 0.1375 0.0 0.0 0.0 0.0 118-119 0.15 0.0 0.0 0.0 0.0 120-121 0.15 0.0 0.0 0.0 0.0 122-123 0.15 0.0 0.0 0.0 0.0 124-125 0.175 0.0 0.0 0.0 0.0 126-127 0.21250000000000002 0.0 0.0 0.0 0.0 128-129 0.2625 0.0 0.0 0.0 0.0 130-131 0.2875 0.0 0.0 0.0 0.0 132-133 0.3125 0.0 0.0 0.0 0.0 134-135 0.4375 0.0 0.0 0.0 0.0 136-137 0.5 0.0 0.0 0.0 0.0 138 0.55 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTCTTT 25 5.904236E-6 115.2 3 TTCTTTT 25 5.904236E-6 115.2 4 TCTTTTC 25 5.904236E-6 115.2 5 GCTTTCT 25 5.904236E-6 115.2 1 CTTTCTT 25 5.904236E-6 115.2 2 TTTTCTT 30 1.46121765E-5 96.0 7 CTTTTCT 40 6.0911432E-5 72.0 6 TTTCTTC 45 1.0917089E-4 64.0 8 TTCTTCA 45 1.0917089E-4 64.0 9 TATATGT 30 0.0015031899 23.999998 20-24 AATTCTT 30 0.0015031899 23.999998 15-19 AAATTCT 30 0.0015031899 23.999998 15-19 TATGTTA 30 0.0015031899 23.999998 25-29 TTATATG 30 0.0015031899 23.999998 20-24 TGTTAGC 30 0.0015031899 23.999998 25-29 CTTATAT 30 0.0015031899 23.999998 20-24 GTTAGCG 30 0.0015031899 23.999998 25-29 ATGTTAG 30 0.0015031899 23.999998 25-29 AAAATTC 30 0.0015031899 23.999998 15-19 ATTCTTA 30 0.0015031899 23.999998 15-19 >>END_MODULE ERR11006609 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006609_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.30175 37.0 37.0 37.0 37.0 37.0 2 36.05 37.0 37.0 37.0 37.0 37.0 3 36.2145 37.0 37.0 37.0 37.0 37.0 4 36.17 37.0 37.0 37.0 37.0 37.0 5 36.399 37.0 37.0 37.0 37.0 37.0 6 36.2405 37.0 37.0 37.0 37.0 37.0 7 36.217 37.0 37.0 37.0 37.0 37.0 8 36.265 37.0 37.0 37.0 37.0 37.0 9 36.3165 37.0 37.0 37.0 37.0 37.0 10-14 36.3246 37.0 37.0 37.0 37.0 37.0 15-19 36.25670000000001 37.0 37.0 37.0 37.0 37.0 20-24 36.2385 37.0 37.0 37.0 37.0 37.0 25-29 36.187599999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.122499999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.102199999999996 37.0 37.0 37.0 37.0 37.0 40-44 36.099900000000005 37.0 37.0 37.0 37.0 37.0 45-49 36.064099999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.040499999999994 37.0 37.0 37.0 37.0 37.0 55-59 35.9965 37.0 37.0 37.0 37.0 37.0 60-64 35.866400000000006 37.0 37.0 37.0 37.0 37.0 65-69 35.8655 37.0 37.0 37.0 37.0 37.0 70-74 35.8621 37.0 37.0 37.0 37.0 37.0 75-79 35.8645 37.0 37.0 37.0 37.0 37.0 80-84 35.7611 37.0 37.0 37.0 37.0 37.0 85-89 35.742399999999996 37.0 37.0 37.0 37.0 37.0 90-94 35.68300000000001 37.0 37.0 37.0 37.0 37.0 95-99 35.568000000000005 37.0 37.0 37.0 37.0 37.0 100-104 35.493700000000004 37.0 37.0 37.0 37.0 37.0 105-109 35.4455 37.0 37.0 37.0 37.0 37.0 110-114 35.3628 37.0 37.0 37.0 32.2 37.0 115-119 35.3441 37.0 37.0 37.0 32.2 37.0 120-124 35.2063 37.0 37.0 37.0 29.8 37.0 125-129 35.2799 37.0 37.0 37.0 32.2 37.0 130-134 35.1331 37.0 37.0 37.0 27.4 37.0 135-139 34.9967 37.0 37.0 37.0 25.0 37.0 140-144 34.94840000000001 37.0 37.0 37.0 25.0 37.0 145-149 34.9928 37.0 37.0 37.0 25.0 37.0 150 34.6475 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 2.0 17 0.0 18 0.0 19 2.0 20 1.0 21 0.0 22 2.0 23 3.0 24 5.0 25 12.0 26 17.0 27 17.0 28 26.0 29 35.0 30 41.0 31 58.0 32 90.0 33 130.0 34 210.0 35 589.0 36 2600.0 37 160.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.285165257494235 25.646938252626185 18.319241609018704 26.74865488086088 2 24.75 24.325 34.5 16.425 3 20.424999999999997 27.525 34.325 17.724999999999998 4 23.425 28.299999999999997 28.925 19.35 5 23.849999999999998 28.775000000000002 28.999999999999996 18.375 6 19.575 32.324999999999996 29.675 18.425 7 20.200000000000003 21.975 40.775 17.05 8 21.15 23.75 32.45 22.650000000000002 9 21.575 20.0 36.925000000000004 21.5 10-14 22.97 25.480000000000004 30.314999999999998 21.235 15-19 23.835 23.985 31.72 20.46 20-24 24.05 23.805 31.25 20.895 25-29 24.240000000000002 24.135 30.89 20.735 30-34 24.745 23.9 30.915 20.44 35-39 24.035 24.03 31.005 20.93 40-44 22.5 25.21 30.485 21.805 45-49 22.465 26.740000000000002 29.65 21.145 50-54 22.275 26.695 28.99 22.040000000000003 55-59 22.985 24.42 29.93 22.665 60-64 22.005 25.15 30.7 22.145 65-69 22.96 25.275 29.875 21.89 70-74 23.335 24.865000000000002 30.97 20.830000000000002 75-79 23.585 25.230000000000004 30.855 20.330000000000002 80-84 24.46 24.5 30.259999999999998 20.78 85-89 24.26 25.91 27.62 22.21 90-94 24.025 24.795 29.115000000000002 22.065 95-99 23.49 24.305 30.459999999999997 21.745 100-104 24.935 23.52 30.5 21.044999999999998 105-109 25.240000000000002 23.01 30.375000000000004 21.375 110-114 24.125 23.165 30.615 22.095000000000002 115-119 23.65 25.35 29.775000000000002 21.224999999999998 120-124 23.26 25.555 28.804999999999996 22.38 125-129 22.375 25.674999999999997 30.240000000000002 21.709999999999997 130-134 21.884999999999998 26.845000000000002 29.205 22.065 135-139 23.195 25.405 30.865 20.535 140-144 23.375 25.130000000000003 30.4 21.095 145-149 22.5 23.87 31.474999999999998 22.155 150 23.724999999999998 23.400000000000002 29.75 23.125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 1.5 23 1.5 24 7.5 25 14.0 26 11.5 27 10.0 28 18.0 29 28.0 30 35.0 31 43.0 32 39.0 33 38.5 34 59.0 35 93.0 36 131.0 37 210.5 38 259.0 39 251.0 40 242.0 41 229.5 42 240.0 43 242.0 44 237.5 45 230.5 46 189.5 47 118.5 48 82.0 49 80.5 50 65.5 51 49.5 52 32.5 53 24.0 54 34.5 55 30.5 56 24.5 57 28.0 58 29.0 59 29.0 60 28.0 61 26.5 62 22.0 63 51.0 64 70.5 65 51.5 66 37.0 67 26.0 68 20.5 69 17.0 70 20.0 71 23.5 72 20.5 73 19.0 74 14.5 75 11.0 76 11.0 77 9.5 78 8.0 79 7.0 80 4.5 81 2.0 82 3.0 83 3.0 84 1.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.4250000000000003 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.25 #Duplication Level Percentage of deduplicated Percentage of total 1 77.08171206225681 49.525000000000006 2 13.618677042801556 17.5 3 4.2412451361867705 8.175 4 1.245136186770428 3.2 5 1.2840466926070038 4.125 6 0.5447470817120622 2.1 7 0.6614785992217899 2.9749999999999996 8 0.11673151750972763 0.6 9 0.3501945525291829 2.025 >10 0.8171206225680935 8.450000000000001 >50 0.038910505836575876 1.325 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 53 1.325 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 32 0.8 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 29 0.7250000000000001 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 25 0.625 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 21 0.525 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 20 0.5 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 20 0.5 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 19 0.475 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 18 0.44999999999999996 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 17 0.42500000000000004 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 13 0.325 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 12 0.3 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 12 0.3 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 12 0.3 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 12 0.3 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 12 0.3 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 11 0.27499999999999997 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 11 0.27499999999999997 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 11 0.27499999999999997 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 11 0.27499999999999997 No Hit AAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATC 10 0.25 No Hit TTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG 10 0.25 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 9 0.22499999999999998 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 9 0.22499999999999998 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 9 0.22499999999999998 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 9 0.22499999999999998 No Hit TATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACC 9 0.22499999999999998 No Hit ATTATCTCTGGTGCTATTATTCCTACTTCTGCGGCAATCGGATTGCACTT 9 0.22499999999999998 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 9 0.22499999999999998 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 9 0.22499999999999998 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 9 0.22499999999999998 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 8 0.2 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 8 0.2 No Hit GCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGC 8 0.2 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 7 0.17500000000000002 No Hit GGTATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATC 7 0.17500000000000002 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 7 0.17500000000000002 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 7 0.17500000000000002 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 7 0.17500000000000002 No Hit ATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATAT 7 0.17500000000000002 No Hit ATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCT 7 0.17500000000000002 No Hit CTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAG 7 0.17500000000000002 No Hit TCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGA 7 0.17500000000000002 No Hit AACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGG 7 0.17500000000000002 No Hit GGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTT 7 0.17500000000000002 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 7 0.17500000000000002 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 7 0.17500000000000002 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 7 0.17500000000000002 No Hit GCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGT 7 0.17500000000000002 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 7 0.17500000000000002 No Hit GTGGACGTTGCCGTAGCGCTGCGGGCCTGGTCTGGGTGTGCTACTGATGG 7 0.17500000000000002 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 6 0.15 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 6 0.15 No Hit GAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAAT 6 0.15 No Hit ATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGATGGTATTCGCGAGCC 6 0.15 No Hit TGTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCAT 6 0.15 No Hit AATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTAT 6 0.15 No Hit CTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATT 6 0.15 No Hit AATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTGAGT 6 0.15 No Hit CAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACTA 6 0.15 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 6 0.15 No Hit CGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAA 6 0.15 No Hit CTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTC 6 0.15 No Hit ATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTG 6 0.15 No Hit CTTTATGATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTC 6 0.15 No Hit CTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGGATGGT 5 0.125 No Hit AAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAA 5 0.125 No Hit GGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTC 5 0.125 No Hit GAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTT 5 0.125 No Hit GTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACC 5 0.125 No Hit AATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACT 5 0.125 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 5 0.125 No Hit GTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTT 5 0.125 No Hit CTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACC 5 0.125 No Hit GACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCC 5 0.125 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 5 0.125 No Hit GCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCC 5 0.125 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 5 0.125 No Hit ATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGATGGTATTC 5 0.125 No Hit CTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTA 5 0.125 No Hit GCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTAT 5 0.125 No Hit CTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAG 5 0.125 No Hit ATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGT 5 0.125 No Hit GTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGT 5 0.125 No Hit GGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTA 5 0.125 No Hit CTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATATTCAGC 5 0.125 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 5 0.125 No Hit TAGCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTT 5 0.125 No Hit CTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGG 5 0.125 No Hit TAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAG 5 0.125 No Hit AGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGG 5 0.125 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 5 0.125 No Hit GTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACA 5 0.125 No Hit AATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTA 5 0.125 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 5 0.125 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 5 0.125 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 5 0.125 No Hit GTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.05 0.0 0.0 0.0 0.0 92-93 0.05 0.0 0.0 0.0 0.0 94-95 0.05 0.0 0.0 0.0 0.0 96-97 0.05 0.0 0.0 0.0 0.0 98-99 0.05 0.0 0.0 0.0 0.0 100-101 0.05 0.0 0.0 0.0 0.0 102-103 0.05 0.0 0.0 0.0 0.0 104-105 0.05 0.0 0.0 0.0 0.0 106-107 0.0875 0.0 0.0 0.0 0.0 108-109 0.125 0.0 0.0 0.0 0.0 110-111 0.125 0.0 0.0 0.0 0.0 112-113 0.125 0.0 0.0 0.0 0.0 114-115 0.125 0.0 0.0 0.0 0.0 116-117 0.1375 0.0 0.0 0.0 0.0 118-119 0.15 0.0 0.0 0.0 0.0 120-121 0.16249999999999998 0.0 0.0 0.0 0.0 122-123 0.175 0.0 0.0 0.0 0.0 124-125 0.1875 0.0 0.0 0.0 0.0 126-127 0.21250000000000002 0.0 0.0 0.0 0.0 128-129 0.2625 0.0 0.0 0.0 0.0 130-131 0.2875 0.0 0.0 0.0 0.0 132-133 0.3125 0.0 0.0 0.0 0.0 134-135 0.4375 0.0 0.0 0.0 0.0 136-137 0.5 0.0 0.0 0.0 0.0 138 0.55 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 3157246 spots for ERR11006609.sra Written 3157246 spots for ERR11006609.sra Read 3157246 spots for ERR11006609.sra Written 3157246 spots for ERR11006609.sra Read 3157246 spots for ERR11006609.sra Written 3157246 spots for ERR11006609.sra Read 3157246 spots for ERR11006609.sra Written 3157246 spots for ERR11006609.sra Read 3157246 spots for ERR11006609.sra Written 3157246 spots for ERR11006609.sra Read 3157246 spots for ERR11006609.sra Written 3157246 spots for ERR11006609.sra Read 3157246 spots for ERR11006609.sra Written 3157246 spots for ERR11006609.sra Read 3157246 spots for ERR11006609.sra Written 3157246 spots for ERR11006609.sra Read 3157246 spots for ERR11006609.sra Written 3157246 spots for ERR11006609.sra Read 3157246 spots for ERR11006609.sra Written 3157246 spots for ERR11006609.sra Read 3157246 spots for ERR11006609.sra Written 3157246 spots for ERR11006609.sra Read 3157246 spots for ERR11006609.sra Written 3157246 spots for ERR11006609.sra Read 3157246 spots for ERR11006609.sra Written 3157246 spots for ERR11006609.sra Read 3157246 spots for ERR11006609.sra Written 3157246 spots for ERR11006609.sra Read 3157246 spots for ERR11006609.sra Written 3157246 spots for ERR11006609.sra Read 3157246 spots for ERR11006609.sra Written 3157246 spots for ERR11006609.sra Read 3157246 spots for ERR11006609.sra Written 3157246 spots for ERR11006609.sra Read 3157246 spots for ERR11006609.sra Written 3157246 spots for ERR11006609.sra Read 3157246 spots for ERR11006609.sra Written 3157246 spots for ERR11006609.sra Read 3157247 spots for ERR11006609.sra Written 3157247 spots for ERR11006609.sra SRR ids: ['ERR11006609.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_u0ljubuu ERR11006609.sra spots: 63144921 blocks: [[1, 3157246], [3157247, 6314492], [6314493, 9471738], [9471739, 12628984], [12628985, 15786230], [15786231, 18943476], [18943477, 22100722], [22100723, 25257968], [25257969, 28415214], [28415215, 31572460], [31572461, 34729706], [34729707, 37886952], [37886953, 41044198], [41044199, 44201444], [44201445, 47358690], [47358691, 50515936], [50515937, 53673182], [53673183, 56830428], [56830429, 59987674], [59987675, 63144921]] ERR11006609 file size 23203918 ERR11006609 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006609 ERR11006609_1.fastq ERR11006609_2.fastq Input file: ERR11006609_1.fastq Paired file: ERR11006609_2.fastq trimmed: ERR11006609-trimmed-pair1.fastq, ERR11006609-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 19:52:43 2024 >> started Fri Dec 6 19:54:00 2024 >> done (77.455s) 63144921 read pairs processed; of these: 238 ( 0.00%) short read pairs filtered out after trimming by size control 2133 ( 0.00%) empty read pairs filtered out after trimming by size control 63142550 (100.00%) read pairs available; of these: 600887 ( 0.95%) trimmed read pairs available after processing 62541663 (99.05%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 14 0.00% 19 10 0.00% 20 15 0.00% 21 19 0.00% 22 33 0.00% 23 12 0.00% 24 7 0.00% 25 22 0.00% 26 13 0.00% 27 17 0.00% 28 14 0.00% 29 29 0.00% 30 24 0.00% 31 96 0.00% 32 21 0.00% 33 24 0.00% 34 15 0.00% 35 21 0.00% 36 22 0.00% 37 17 0.00% 38 19 0.00% 39 23 0.00% 40 38 0.00% 41 34 0.00% 42 42 0.00% 43 27 0.00% 44 45 0.00% 45 37 0.00% 46 51 0.00% 47 54 0.00% 48 42 0.00% 49 58 0.00% 50 47 0.00% 51 73 0.00% 52 60 0.00% 53 76 0.00% 54 58 0.00% 55 63 0.00% 56 92 0.00% 57 102 0.00% 58 110 0.00% 59 98 0.00% 60 114 0.00% 61 124 0.00% 62 184 0.00% 63 200 0.00% 64 171 0.00% 65 238 0.00% 66 209 0.00% 67 282 0.00% 68 240 0.00% 69 269 0.00% 70 315 0.00% 71 345 0.00% 72 369 0.00% 73 441 0.00% 74 440 0.00% 75 468 0.00% 76 532 0.00% 77 528 0.00% 78 577 0.00% 79 648 0.00% 80 597 0.00% 81 727 0.00% 82 742 0.00% 83 799 0.00% 84 909 0.00% 85 1088 0.00% 86 1150 0.00% 87 1357 0.00% 88 1334 0.00% 89 1404 0.00% 90 1500 0.00% 91 1540 0.00% 92 1622 0.00% 93 1693 0.00% 94 1876 0.00% 95 2094 0.00% 96 2109 0.00% 97 2207 0.00% 98 2379 0.00% 99 2511 0.00% 100 2761 0.00% 101 2819 0.00% 102 2884 0.00% 103 3194 0.01% 104 3323 0.01% 105 3616 0.01% 106 3904 0.01% 107 4065 0.01% 108 4250 0.01% 109 4443 0.01% 110 4726 0.01% 111 4956 0.01% 112 5560 0.01% 113 5429 0.01% 114 5527 0.01% 115 5939 0.01% 116 6565 0.01% 117 6833 0.01% 118 6747 0.01% 119 7197 0.01% 120 8226 0.01% 121 8573 0.01% 122 9500 0.02% 123 9385 0.01% 124 10238 0.02% 125 11456 0.02% 126 10213 0.02% 127 9905 0.02% 128 10724 0.02% 129 11928 0.02% 130 12718 0.02% 131 13529 0.02% 132 14140 0.02% 133 13368 0.02% 134 14084 0.02% 135 13778 0.02% 136 14560 0.02% 137 14767 0.02% 138 15646 0.02% 139 16860 0.03% 140 16917 0.03% 141 18587 0.03% 142 19121 0.03% 143 20613 0.03% 144 20192 0.03% 145 22928 0.04% 146 27775 0.04% 147 25001 0.04% 148 26063 0.04% 149 27328 0.04% 150 62541663 99.05% 63142550 reads passed initial QC criterion=sequence-density sequence-density=0.34 sequence-density-rank=1 fanout-score=0.00 fanout-score-rank=32 prefix-density=0.00 prefix-fanout=1.0 sequence=GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTTATCCTTCCACCGTTGGAAGCGGGCAGTTGTCGCTGCTCTGTGAAGCCAGCCTCACGCTGTGCCTGCCAACATTATGGGCCGCGAAGCCTAGCTTTCGCTTAAGCTCCAACGGCCCACTACGCAACTTGGAACGGGCGGGCCATCAGTAGCACACCCAGACCAGGCCCGCAGCGCTACGGCAACGTCCACACCACCCTTAAAGCCCCCACTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=44 fanout-score=54.22 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=1.0 sequence=AGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCCCTTGCCTACATTGTTCCATTGGCCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGAACGGTACTCGGTCCTCCGGATTTTCATGGGCCGCCGGGGGCGCACCGGACACCGCGCGACGTGCGGTGCTCTTCCGGCCACTGGACCCTACCTCCGGCTGAACCGATTCCAGGGTTGGCAGGCCGTTAAGCAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGGCGTCTCCGGACTTCCTAACGTCGCCGTCAACCGCCACATCCCGGCTCGG criterion=sequence-density sequence-density=0.33 sequence-density-rank=1 fanout-score=1.92 fanout-score-rank=34 prefix-density=0.33 prefix-fanout=1.9 sequence=GCGGCTCTCCTA criterion=fanout-score sequence-density=0.11 sequence-density-rank=12 fanout-score=109.47 fanout-score-rank=1 prefix-density=11.93 prefix-fanout=1.0 sequence=CTGGATAACTATCACTGAAAATC ERR11006609 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 19:54:53 Started mapping on | Dec 06 19:54:54 Finished on | Dec 06 20:01:43 Mapping speed, Million of reads per hour | 555.78 Number of input reads | 63142550 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 48321869 Uniquely mapped reads % | 76.53% Average mapped length | 298.45 Number of splices: Total | 13134358 Number of splices: Annotated (sjdb) | 12159393 Number of splices: GT/AG | 12758184 Number of splices: GC/AG | 142474 Number of splices: AT/AC | 22672 Number of splices: Non-canonical | 211028 Mismatch rate per base, % | 0.30% Deletion rate per base | 0.01% Deletion average length | 1.62 Insertion rate per base | 0.01% Insertion average length | 2.16 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 12894362 % of reads mapped to multiple loci | 20.42% Number of reads mapped to too many loci | 10182 % of reads mapped to too many loci | 0.02% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.70% % of reads unmapped: other | 0.34% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1926319 1926319 1926319 N_multimapping 12894362 12894362 12894362 N_noFeature 12598399 44872655 14936049 N_ambiguous 1810124 49405 695673 UnstrandedReadsAssigned:33913346 PositiveStrandReadsAssigned:3399809 NegativeStrandReadsAssigned:32690147 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006609 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006609-trimmed-pair1.fastq ERR11006609-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 63,142,550 reads, 35,205,103 reads pseudoaligned [quant] estimated average fragment length: 267.682 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,076 rounds 52973 ERR11006609.ke.tsv 35125 ERR11006609.se.tsv 88098 total ==> ERR11006609.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 669.624 0 0 PNS24247 1044 777.318 20.0034 0.668129 PNS24249 1928 1661.32 163.536 2.55574 PNS24246 1044 777.318 20.0034 0.668129 PNS24248 1044 777.318 20.0034 0.668129 PNS24244 1471 1204.32 15.4537 0.333155 PNS24243 293 54.5784 0 0 KQK14069 1603 1336.32 1322.37 25.692 KQK14071 474 209.573 3.93426 0.487398 ==> ERR11006609.se.tsv <== BRADI_1g14170v3 1359 BRADI_1g53295v3 376 BRADI_1g59795v3 232 BRADI_1g07683v3 1 BRADI_1g00485v3 1 BRADI_1g20270v3 216 BRADI_1g74790v3 337 BRADI_1g09890v3 0 BRADI_1g77505v3 92 BRADI_1g48960v3 0 ERR11006609 completed mapping pipeline successfully