Starting /dee2/code/volunteer_pipeline.sh ERR11006610 current disk space = 1549611667456 free memory = 1596852896 ERR11006610 SRAfilesize bd7737ba7a9c77496e99fa861a142f76 ERR11006610.sra ERR11006610.sra file validated ERR11006610 is paired end ERR11006610 is conventional basespace ERR11006610 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006610_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.6435 37.0 37.0 37.0 37.0 37.0 2 35.7525 37.0 37.0 37.0 37.0 37.0 3 36.067 37.0 37.0 37.0 37.0 37.0 4 36.089 37.0 37.0 37.0 37.0 37.0 5 36.158 37.0 37.0 37.0 37.0 37.0 6 36.141 37.0 37.0 37.0 37.0 37.0 7 36.0795 37.0 37.0 37.0 37.0 37.0 8 35.984 37.0 37.0 37.0 37.0 37.0 9 36.132 37.0 37.0 37.0 37.0 37.0 10-14 36.199 37.0 37.0 37.0 37.0 37.0 15-19 36.2074 37.0 37.0 37.0 37.0 37.0 20-24 36.167899999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.0818 37.0 37.0 37.0 37.0 37.0 30-34 36.061 37.0 37.0 37.0 37.0 37.0 35-39 36.0072 37.0 37.0 37.0 37.0 37.0 40-44 35.976299999999995 37.0 37.0 37.0 37.0 37.0 45-49 35.98969999999999 37.0 37.0 37.0 37.0 37.0 50-54 35.922399999999996 37.0 37.0 37.0 37.0 37.0 55-59 35.9217 37.0 37.0 37.0 37.0 37.0 60-64 35.82809999999999 37.0 37.0 37.0 37.0 37.0 65-69 35.8569 37.0 37.0 37.0 37.0 37.0 70-74 35.8099 37.0 37.0 37.0 37.0 37.0 75-79 35.8264 37.0 37.0 37.0 37.0 37.0 80-84 35.7002 37.0 37.0 37.0 37.0 37.0 85-89 35.687400000000004 37.0 37.0 37.0 37.0 37.0 90-94 35.5348 37.0 37.0 37.0 37.0 37.0 95-99 35.5606 37.0 37.0 37.0 37.0 37.0 100-104 35.3891 37.0 37.0 37.0 34.6 37.0 105-109 35.4209 37.0 37.0 37.0 34.6 37.0 110-114 35.565200000000004 37.0 37.0 37.0 37.0 37.0 115-119 35.490199999999994 37.0 37.0 37.0 37.0 37.0 120-124 35.155100000000004 37.0 37.0 37.0 29.8 37.0 125-129 35.194100000000006 37.0 37.0 37.0 29.8 37.0 130-134 35.091899999999995 37.0 37.0 37.0 25.0 37.0 135-139 35.0528 37.0 37.0 37.0 25.0 37.0 140-144 34.7928 37.0 37.0 37.0 25.0 37.0 145-149 35.017999999999994 37.0 37.0 37.0 25.0 37.0 150 34.8695 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 1.0 22 1.0 23 3.0 24 2.0 25 3.0 26 6.0 27 10.0 28 28.0 29 38.0 30 68.0 31 59.0 32 105.0 33 141.0 34 237.0 35 670.0 36 2543.0 37 84.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.675 13.125 15.075 32.125 2 29.989969909729187 10.606820461384151 27.331995987963893 32.07121364092277 3 23.425 13.375 26.424999999999997 36.775000000000006 4 27.388694347173587 15.907953976988495 24.387193596798397 32.31615807903952 5 28.95 18.725 25.650000000000002 26.674999999999997 6 25.624999999999996 27.800000000000004 21.65 24.925 7 17.65 30.9 33.25 18.2 8 17.375 28.525 32.975 21.125 9 18.9 27.400000000000002 32.85 20.849999999999998 10-14 20.995 31.435000000000002 26.295 21.275 15-19 21.68 32.0 24.295 22.025 20-24 20.225 28.725 27.229999999999997 23.82 25-29 22.884999999999998 30.775000000000002 24.785 21.555 30-34 24.62 29.975 23.82 21.584999999999997 35-39 23.015 30.55 24.505 21.93 40-44 21.51 29.659999999999997 25.465 23.365 45-49 21.39 28.96 26.22 23.43 50-54 21.88 31.09 25.055 21.975 55-59 22.685 29.07 23.94 24.305 60-64 21.33 30.064999999999998 24.615000000000002 23.990000000000002 65-69 22.509999999999998 29.575000000000003 23.955000000000002 23.96 70-74 23.64 29.415000000000003 22.384999999999998 24.560000000000002 75-79 22.58 29.080000000000002 24.75 23.59 80-84 23.835 28.03 24.6 23.535 85-89 23.995 28.345 25.025 22.634999999999998 90-94 22.115000000000002 28.96 24.915000000000003 24.01 95-99 24.86 28.965000000000003 22.675 23.5 100-104 22.805 29.415000000000003 23.974999999999998 23.805 105-109 22.439999999999998 27.875 25.740000000000002 23.945 110-114 23.369999999999997 27.700000000000003 24.575 24.355 115-119 21.675 29.78 24.654999999999998 23.89 120-124 20.28 29.15 25.2 25.369999999999997 125-129 22.215 30.159999999999997 22.235 25.39 130-134 22.939999999999998 29.54 24.275 23.244999999999997 135-139 24.005000000000003 28.73 22.75 24.515 140-144 25.155 27.83 25.014999999999997 22.0 145-149 23.23 28.935 24.83 23.005 150 24.825 26.025 25.6 23.549999999999997 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 0.0 22 0.5 23 2.0 24 4.0 25 3.5 26 2.0 27 7.0 28 9.5 29 12.5 30 16.5 31 19.5 32 25.5 33 35.5 34 46.0 35 52.0 36 84.5 37 147.5 38 241.0 39 252.0 40 226.5 41 252.0 42 241.5 43 237.0 44 230.0 45 224.5 46 192.5 47 153.0 48 135.5 49 100.5 50 79.0 51 64.5 52 53.0 53 42.5 54 36.5 55 34.0 56 33.0 57 31.5 58 29.0 59 37.0 60 39.5 61 36.5 62 30.0 63 26.0 64 54.5 65 77.0 66 55.5 67 32.0 68 28.0 69 24.0 70 21.5 71 25.5 72 26.0 73 21.0 74 21.5 75 21.5 76 15.0 77 10.0 78 6.0 79 9.5 80 12.5 81 6.5 82 2.0 83 1.5 84 1.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.3 3 0.0 4 0.05 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.8 #Duplication Level Percentage of deduplicated Percentage of total 1 83.43023255813954 57.4 2 10.719476744186046 14.75 3 2.3255813953488373 4.8 4 0.6540697674418605 1.7999999999999998 5 0.5450581395348837 1.875 6 0.5087209302325582 2.1 7 0.3633720930232558 1.7500000000000002 8 0.29069767441860467 1.6 9 0.2543604651162791 1.575 >10 0.872093023255814 10.05 >50 0.036337209302325583 2.3 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 92 2.3 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 38 0.95 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 32 0.8 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 27 0.675 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 27 0.675 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 26 0.65 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 25 0.625 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 19 0.475 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 17 0.42500000000000004 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 17 0.42500000000000004 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 16 0.4 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 14 0.35000000000000003 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 14 0.35000000000000003 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 13 0.325 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 13 0.325 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 12 0.3 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 11 0.27499999999999997 No Hit CGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTG 11 0.27499999999999997 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 10 0.25 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 10 0.25 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 10 0.25 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 10 0.25 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 10 0.25 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 10 0.25 No Hit TAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGA 10 0.25 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 9 0.22499999999999998 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 9 0.22499999999999998 No Hit GCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGG 9 0.22499999999999998 No Hit GTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCG 9 0.22499999999999998 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 9 0.22499999999999998 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 9 0.22499999999999998 No Hit GGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGG 9 0.22499999999999998 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 8 0.2 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 8 0.2 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 8 0.2 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 8 0.2 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 8 0.2 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 8 0.2 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 8 0.2 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 8 0.2 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 7 0.17500000000000002 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 7 0.17500000000000002 No Hit ACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 7 0.17500000000000002 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 7 0.17500000000000002 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 7 0.17500000000000002 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 7 0.17500000000000002 No Hit ATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAG 7 0.17500000000000002 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 7 0.17500000000000002 No Hit CAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCA 7 0.17500000000000002 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 7 0.17500000000000002 No Hit GGGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACA 6 0.15 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 6 0.15 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 6 0.15 No Hit TTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTA 6 0.15 No Hit TGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGA 6 0.15 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 6 0.15 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 6 0.15 No Hit GGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAG 6 0.15 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 6 0.15 No Hit ATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATA 6 0.15 No Hit CTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCC 6 0.15 No Hit CAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGA 6 0.15 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 6 0.15 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 6 0.15 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 5 0.125 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 5 0.125 No Hit TGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATA 5 0.125 No Hit CCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTT 5 0.125 No Hit CTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAG 5 0.125 No Hit GCAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAA 5 0.125 No Hit AATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTT 5 0.125 No Hit CCCACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACA 5 0.125 No Hit GGCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGAT 5 0.125 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 5 0.125 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 5 0.125 No Hit GCCTAAAGTTAAGGATTTATCAATGGGTAATGTTGCTCCAATACCTAACC 5 0.125 No Hit GCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAGCTGCAA 5 0.125 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 5 0.125 No Hit CACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0125 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.05 0.0 0.0 0.0 0.0 92-93 0.05 0.0 0.0 0.0 0.0 94-95 0.05 0.0 0.0 0.0 0.0 96-97 0.05 0.0 0.0 0.0 0.0 98-99 0.075 0.0 0.0 0.0 0.0 100-101 0.075 0.0 0.0 0.0 0.0 102-103 0.1 0.0 0.0 0.0 0.0 104-105 0.1 0.0 0.0 0.0 0.0 106-107 0.1 0.0 0.0 0.0 0.0 108-109 0.1125 0.0 0.0 0.0 0.0 110-111 0.125 0.0 0.0 0.0 0.0 112-113 0.125 0.0 0.0 0.0 0.0 114-115 0.125 0.0 0.0 0.0 0.0 116-117 0.125 0.0 0.0 0.0 0.0 118-119 0.15 0.0 0.0 0.0 0.0 120-121 0.175 0.0 0.0 0.0 0.0 122-123 0.21250000000000002 0.0 0.0 0.0 0.0 124-125 0.2625 0.0 0.0 0.0 0.0 126-127 0.3125 0.0 0.0 0.0 0.0 128-129 0.3625 0.0 0.0 0.0 0.0 130-131 0.42500000000000004 0.0 0.0 0.0 0.0 132-133 0.4625 0.0 0.0 0.0 0.0 134-135 0.5 0.0 0.0 0.0 0.0 136-137 0.525 0.0 0.0 0.0 0.0 138 0.575 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCTTTC 15 1.1730364E-4 144.0 1 CTTTTTA 10 0.006973645 144.0 6 TCTTTTC 20 3.687869E-4 108.0 6 CTTTTCT 25 8.956223E-4 86.399994 7 GCTTTCT 25 8.956223E-4 86.399994 2 CTTTCTT 25 8.956223E-4 86.399994 3 TTTCTTT 30 0.0018473949 72.0 4 TTCTTTT 30 0.0018473949 72.0 4 TTTCTTC 35 0.0034045284 61.714283 9 TTTTCTT 35 0.0034045284 61.714283 8 CTTCAAA 20 0.006139246 28.8 10-14 AATTCTT 20 0.006139246 28.8 15-19 TTCAAAA 20 0.006139246 28.8 10-14 TATGTTA 20 0.006139246 28.8 25-29 TGTTAGC 20 0.006139246 28.8 25-29 TCAAAAA 20 0.006139246 28.8 10-14 TTCTTAT 25 5.183459E-4 28.8 20-24 CTTATAT 25 5.183459E-4 28.8 20-24 ATATGTT 20 0.006139246 28.8 25-29 ATGTTAG 20 0.006139246 28.8 25-29 >>END_MODULE ERR11006610 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006610_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.35525 37.0 37.0 37.0 37.0 37.0 2 35.857 37.0 37.0 37.0 37.0 37.0 3 35.622 37.0 37.0 37.0 37.0 37.0 4 35.8475 37.0 37.0 37.0 37.0 37.0 5 35.8025 37.0 37.0 37.0 37.0 37.0 6 35.464 37.0 37.0 37.0 37.0 37.0 7 35.601 37.0 37.0 37.0 37.0 37.0 8 35.5885 37.0 37.0 37.0 37.0 37.0 9 35.587 37.0 37.0 37.0 37.0 37.0 10-14 35.620999999999995 37.0 37.0 37.0 37.0 37.0 15-19 35.6477 37.0 37.0 37.0 37.0 37.0 20-24 35.5514 37.0 37.0 37.0 37.0 37.0 25-29 35.4716 37.0 37.0 37.0 37.0 37.0 30-34 35.4433 37.0 37.0 37.0 37.0 37.0 35-39 35.4239 37.0 37.0 37.0 37.0 37.0 40-44 35.368300000000005 37.0 37.0 37.0 34.6 37.0 45-49 35.28920000000001 37.0 37.0 37.0 34.6 37.0 50-54 35.265100000000004 37.0 37.0 37.0 29.8 37.0 55-59 35.320499999999996 37.0 37.0 37.0 32.2 37.0 60-64 35.2234 37.0 37.0 37.0 29.8 37.0 65-69 35.2108 37.0 37.0 37.0 29.8 37.0 70-74 35.2331 37.0 37.0 37.0 29.8 37.0 75-79 35.071000000000005 37.0 37.0 37.0 25.0 37.0 80-84 35.0941 37.0 37.0 37.0 29.8 37.0 85-89 34.994899999999994 37.0 37.0 37.0 25.0 37.0 90-94 34.9838 37.0 37.0 37.0 25.0 37.0 95-99 34.908 37.0 37.0 37.0 25.0 37.0 100-104 34.8823 37.0 37.0 37.0 25.0 37.0 105-109 34.689800000000005 37.0 37.0 37.0 25.0 37.0 110-114 34.5831 37.0 37.0 37.0 25.0 37.0 115-119 34.5228 37.0 37.0 37.0 25.0 37.0 120-124 34.42380000000001 37.0 37.0 37.0 25.0 37.0 125-129 34.3416 37.0 37.0 37.0 25.0 37.0 130-134 34.491299999999995 37.0 37.0 37.0 25.0 37.0 135-139 34.317499999999995 37.0 37.0 37.0 25.0 37.0 140-144 34.1197 37.0 37.0 37.0 25.0 37.0 145-149 34.2593 37.0 37.0 37.0 25.0 37.0 150 34.2985 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 2.0 19 1.0 20 0.0 21 9.0 22 9.0 23 10.0 24 7.0 25 16.0 26 19.0 27 25.0 28 31.0 29 54.0 30 69.0 31 99.0 32 148.0 33 240.0 34 437.0 35 1064.0 36 1712.0 37 47.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 30.57851239669421 23.716503881793138 18.056599048334583 27.64838467317806 2 29.349999999999998 24.175 29.925 16.55 3 23.925 25.650000000000002 30.725 19.7 4 26.1 28.775000000000002 25.0 20.125 5 25.7 27.775 28.375 18.15 6 20.825 30.9 27.825 20.45 7 20.45 20.575 39.725 19.25 8 23.0 22.125 30.325000000000003 24.55 9 23.9 19.975 33.6 22.525000000000002 10-14 23.57 25.955000000000002 28.04 22.435 15-19 24.375 24.425 29.92 21.279999999999998 20-24 25.480000000000004 23.75 29.535 21.235 25-29 24.654999999999998 24.27 29.225 21.85 30-34 24.959999999999997 24.21 29.07 21.759999999999998 35-39 24.815 23.94 28.87 22.375 40-44 23.54 24.925 29.54 21.995 45-49 23.21 26.11 28.505000000000003 22.175 50-54 23.715 25.629999999999995 28.189999999999998 22.465 55-59 23.745 24.41 28.244999999999997 23.599999999999998 60-64 23.575 24.25 29.049999999999997 23.125 65-69 24.235 23.995 28.87 22.900000000000002 70-74 24.97 24.83 28.384999999999998 21.815 75-79 24.884999999999998 24.315 29.354999999999997 21.445 80-84 25.180000000000003 24.545 29.07 21.205 85-89 25.75 24.675 26.69 22.884999999999998 90-94 24.22 23.9 28.465 23.415 95-99 23.849999999999998 24.52 28.305000000000003 23.325000000000003 100-104 24.75 23.61 29.985 21.654999999999998 105-109 26.91 22.89 28.53 21.67 110-114 24.585 23.244999999999997 29.294999999999998 22.875 115-119 24.435000000000002 24.565 28.76 22.24 120-124 24.48 24.46 28.475 22.585 125-129 23.91 24.884999999999998 28.165000000000003 23.04 130-134 23.69 25.669999999999998 27.71 22.93 135-139 24.51 25.44 28.37 21.68 140-144 24.42 24.635 29.25 21.695 145-149 23.849999999999998 24.104999999999997 29.349999999999998 22.695 150 24.15 23.0 28.075 24.775 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.0 20 1.0 21 1.0 22 2.0 23 3.5 24 4.0 25 7.5 26 13.0 27 13.0 28 15.0 29 16.5 30 16.0 31 23.0 32 27.0 33 29.5 34 57.5 35 76.5 36 85.5 37 167.5 38 223.5 39 202.0 40 205.5 41 225.0 42 221.5 43 214.0 44 219.0 45 202.0 46 186.0 47 162.5 48 118.5 49 98.0 50 86.0 51 62.5 52 51.5 53 50.0 54 41.5 55 39.0 56 39.0 57 37.5 58 39.5 59 45.5 60 44.5 61 34.5 62 32.0 63 54.5 64 74.0 65 67.5 66 45.5 67 30.5 68 34.0 69 36.0 70 33.0 71 30.0 72 21.5 73 23.0 74 24.0 75 17.5 76 12.5 77 8.5 78 11.0 79 10.0 80 7.0 81 5.0 82 3.5 83 3.0 84 2.0 85 1.5 86 0.5 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17500000000000002 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.5 #Duplication Level Percentage of deduplicated Percentage of total 1 84.14965986394559 61.85000000000001 2 9.795918367346939 14.399999999999999 3 2.380952380952381 5.25 4 1.0884353741496597 3.2 5 0.9183673469387756 3.375 6 0.5782312925170068 2.55 7 0.3401360544217687 1.7500000000000002 8 0.10204081632653061 0.6 9 0.17006802721088435 1.125 >10 0.44217687074829937 4.5 >50 0.03401360544217687 1.4000000000000001 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 56 1.4000000000000001 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 22 0.5499999999999999 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 20 0.5 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 15 0.375 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 15 0.375 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 14 0.35000000000000003 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 14 0.35000000000000003 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 14 0.35000000000000003 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 12 0.3 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 11 0.27499999999999997 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 11 0.27499999999999997 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 11 0.27499999999999997 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 11 0.27499999999999997 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 10 0.25 No Hit TTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAA 9 0.22499999999999998 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 9 0.22499999999999998 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 9 0.22499999999999998 No Hit GGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTT 9 0.22499999999999998 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 9 0.22499999999999998 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 8 0.2 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 8 0.2 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 8 0.2 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 7 0.17500000000000002 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 7 0.17500000000000002 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 7 0.17500000000000002 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 7 0.17500000000000002 No Hit AGCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTG 7 0.17500000000000002 No Hit CCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGA 7 0.17500000000000002 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 7 0.17500000000000002 No Hit TTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGG 7 0.17500000000000002 No Hit GAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCG 7 0.17500000000000002 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 7 0.17500000000000002 No Hit GGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTC 6 0.15 No Hit GTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTAT 6 0.15 No Hit AGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAA 6 0.15 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 6 0.15 No Hit TGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGCTAGTT 6 0.15 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 6 0.15 No Hit ATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATAT 6 0.15 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 6 0.15 No Hit TCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGA 6 0.15 No Hit TATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACC 6 0.15 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 6 0.15 No Hit CTCCTGTTGCAGCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAA 6 0.15 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 6 0.15 No Hit ATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTAC 6 0.15 No Hit TAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTC 6 0.15 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 6 0.15 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 6 0.15 No Hit AAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATC 5 0.125 No Hit TGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGA 5 0.125 No Hit CCGCAACTTCTGTATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGAT 5 0.125 No Hit CCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGG 5 0.125 No Hit GCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCA 5 0.125 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 5 0.125 No Hit ATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCAT 5 0.125 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 5 0.125 No Hit ATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCT 5 0.125 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 5 0.125 No Hit ATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGATGGTATTC 5 0.125 No Hit AGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAG 5 0.125 No Hit GTAGCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGT 5 0.125 No Hit ATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGT 5 0.125 No Hit AGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCT 5 0.125 No Hit TATGCCTTTAGGAATATCTGGTACTTTCAACTTTATGATTGTATTCCAGG 5 0.125 No Hit CTTGATTTACCCTATTGGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAG 5 0.125 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 5 0.125 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 5 0.125 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 5 0.125 No Hit GCTGCGACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGCTTCTC 5 0.125 No Hit ATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTCACATGTT 5 0.125 No Hit GTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACA 5 0.125 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 5 0.125 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 5 0.125 No Hit GAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTA 5 0.125 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0125 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.05 0.0 0.0 0.0 0.0 92-93 0.05 0.0 0.0 0.0 0.0 94-95 0.05 0.0 0.0 0.0 0.0 96-97 0.05 0.0 0.0 0.0 0.0 98-99 0.075 0.0 0.0 0.0 0.0 100-101 0.075 0.0 0.0 0.0 0.0 102-103 0.1 0.0 0.0 0.0 0.0 104-105 0.1 0.0 0.0 0.0 0.0 106-107 0.1 0.0 0.0 0.0 0.0 108-109 0.1125 0.0 0.0 0.0 0.0 110-111 0.125 0.0 0.0 0.0 0.0 112-113 0.125 0.0 0.0 0.0 0.0 114-115 0.125 0.0 0.0 0.0 0.0 116-117 0.125 0.0 0.0 0.0 0.0 118-119 0.15 0.0 0.0 0.0 0.0 120-121 0.175 0.0 0.0 0.0 0.0 122-123 0.21250000000000002 0.0 0.0 0.0 0.0 124-125 0.25 0.0 0.0 0.0 0.0 126-127 0.2875 0.0 0.0 0.0 0.0 128-129 0.35 0.0 0.0 0.0 0.0 130-131 0.42500000000000004 0.0 0.0 0.0 0.0 132-133 0.4625 0.0 0.0 0.0 0.0 134-135 0.5 0.0 0.0 0.0 0.0 136-137 0.55 0.0 0.0 0.0 0.0 138 0.575 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGAAGC 10 0.006973645 144.0 1 >>END_MODULE Read 2587673 spots for ERR11006610.sra Written 2587673 spots for ERR11006610.sra Read 2587673 spots for ERR11006610.sra Written 2587673 spots for ERR11006610.sra Read 2587673 spots for ERR11006610.sra Written 2587673 spots for ERR11006610.sra Read 2587673 spots for ERR11006610.sra Written 2587673 spots for ERR11006610.sra Read 2587673 spots for ERR11006610.sra Written 2587673 spots for ERR11006610.sra Read 2587673 spots for ERR11006610.sra Written 2587673 spots for ERR11006610.sra Read 2587673 spots for ERR11006610.sra Written 2587673 spots for ERR11006610.sra Read 2587673 spots for ERR11006610.sra Written 2587673 spots for ERR11006610.sra Read 2587673 spots for ERR11006610.sra Written 2587673 spots for ERR11006610.sra Read 2587673 spots for ERR11006610.sra Written 2587673 spots for ERR11006610.sra Read 2587673 spots for ERR11006610.sra Written 2587673 spots for ERR11006610.sra Read 2587673 spots for ERR11006610.sra Written 2587673 spots for ERR11006610.sra Read 2587673 spots for ERR11006610.sra Written 2587673 spots for ERR11006610.sra Read 2587673 spots for ERR11006610.sra Written 2587673 spots for ERR11006610.sra Read 2587673 spots for ERR11006610.sra Written 2587673 spots for ERR11006610.sra Read 2587673 spots for ERR11006610.sra Written 2587673 spots for ERR11006610.sra Read 2587673 spots for ERR11006610.sra Written 2587673 spots for ERR11006610.sra Read 2587673 spots for ERR11006610.sra Written 2587673 spots for ERR11006610.sra Read 2587673 spots for ERR11006610.sra Written 2587673 spots for ERR11006610.sra Read 2587675 spots for ERR11006610.sra Written 2587675 spots for ERR11006610.sra SRR ids: ['ERR11006610.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd__elpo04o ERR11006610.sra spots: 51753462 blocks: [[1, 2587673], [2587674, 5175346], [5175347, 7763019], [7763020, 10350692], [10350693, 12938365], [12938366, 15526038], [15526039, 18113711], [18113712, 20701384], [20701385, 23289057], [23289058, 25876730], [25876731, 28464403], [28464404, 31052076], [31052077, 33639749], [33639750, 36227422], [36227423, 38815095], [38815096, 41402768], [41402769, 43990441], [43990442, 46578114], [46578115, 49165787], [49165788, 51753462]] ERR11006610 file size 19015924 ERR11006610 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006610 ERR11006610_1.fastq ERR11006610_2.fastq Input file: ERR11006610_1.fastq Paired file: ERR11006610_2.fastq trimmed: ERR11006610-trimmed-pair1.fastq, ERR11006610-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 19:55:50 2024 >> started Fri Dec 6 19:57:53 2024 >> done (122.893s) 51753462 read pairs processed; of these: 152 ( 0.00%) short read pairs filtered out after trimming by size control 1593 ( 0.00%) empty read pairs filtered out after trimming by size control 51751717 (100.00%) read pairs available; of these: 400088 ( 0.77%) trimmed read pairs available after processing 51351629 (99.23%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 5 0.00% 19 8 0.00% 20 7 0.00% 21 11 0.00% 22 14 0.00% 23 8 0.00% 24 9 0.00% 25 12 0.00% 26 11 0.00% 27 14 0.00% 28 18 0.00% 29 26 0.00% 30 20 0.00% 31 74 0.00% 32 23 0.00% 33 22 0.00% 34 12 0.00% 35 19 0.00% 36 20 0.00% 37 11 0.00% 38 27 0.00% 39 21 0.00% 40 19 0.00% 41 21 0.00% 42 21 0.00% 43 31 0.00% 44 21 0.00% 45 25 0.00% 46 27 0.00% 47 41 0.00% 48 24 0.00% 49 28 0.00% 50 37 0.00% 51 36 0.00% 52 48 0.00% 53 45 0.00% 54 40 0.00% 55 63 0.00% 56 89 0.00% 57 65 0.00% 58 65 0.00% 59 73 0.00% 60 75 0.00% 61 80 0.00% 62 89 0.00% 63 106 0.00% 64 106 0.00% 65 130 0.00% 66 135 0.00% 67 144 0.00% 68 155 0.00% 69 172 0.00% 70 163 0.00% 71 208 0.00% 72 219 0.00% 73 239 0.00% 74 251 0.00% 75 297 0.00% 76 308 0.00% 77 319 0.00% 78 368 0.00% 79 382 0.00% 80 405 0.00% 81 468 0.00% 82 510 0.00% 83 472 0.00% 84 549 0.00% 85 612 0.00% 86 679 0.00% 87 762 0.00% 88 791 0.00% 89 888 0.00% 90 959 0.00% 91 942 0.00% 92 1041 0.00% 93 1076 0.00% 94 1156 0.00% 95 1238 0.00% 96 1271 0.00% 97 1374 0.00% 98 1516 0.00% 99 1570 0.00% 100 1676 0.00% 101 1743 0.00% 102 1763 0.00% 103 2047 0.00% 104 2100 0.00% 105 2266 0.00% 106 2448 0.00% 107 2517 0.00% 108 2740 0.01% 109 2811 0.01% 110 3032 0.01% 111 3081 0.01% 112 3340 0.01% 113 3484 0.01% 114 3549 0.01% 115 3967 0.01% 116 4208 0.01% 117 4388 0.01% 118 4503 0.01% 119 4707 0.01% 120 5354 0.01% 121 5332 0.01% 122 5720 0.01% 123 6023 0.01% 124 6273 0.01% 125 7056 0.01% 126 6567 0.01% 127 6543 0.01% 128 7180 0.01% 129 7793 0.02% 130 8256 0.02% 131 8968 0.02% 132 9644 0.02% 133 9203 0.02% 134 9650 0.02% 135 9385 0.02% 136 10289 0.02% 137 10362 0.02% 138 10969 0.02% 139 11644 0.02% 140 12159 0.02% 141 12761 0.02% 142 13260 0.03% 143 13948 0.03% 144 14362 0.03% 145 15745 0.03% 146 17894 0.03% 147 17357 0.03% 148 17476 0.03% 149 19109 0.04% 150 51351629 99.23% 51751717 reads passed initial QC criterion=sequence-density sequence-density=0.30 sequence-density-rank=1 fanout-score=0.00 fanout-score-rank=28 prefix-density=0.00 prefix-fanout=1.0 sequence=TGCGGGAACTTC criterion=fanout-score sequence-density=0.08 sequence-density-rank=18 fanout-score=123.72 fanout-score-rank=1 prefix-density=9.37 prefix-fanout=1.0 sequence=ATCTAGAGGGACGTTGTGAGCAT criterion=sequence-density sequence-density=0.21 sequence-density-rank=1 fanout-score=47.84 fanout-score-rank=9 prefix-density=9.72 prefix-fanout=1.0 sequence=GCACTGAAAATAGTCTTTACAT criterion=fanout-score sequence-density=0.08 sequence-density-rank=29 fanout-score=116.11 fanout-score-rank=1 prefix-density=9.43 prefix-fanout=1.0 sequence=ATAACTAGCACAGAAAATCGTCT ERR11006610 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 19:58:38 Started mapping on | Dec 06 19:58:39 Finished on | Dec 06 20:08:34 Mapping speed, Million of reads per hour | 313.12 Number of input reads | 51751717 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 41386000 Uniquely mapped reads % | 79.97% Average mapped length | 298.41 Number of splices: Total | 16297032 Number of splices: Annotated (sjdb) | 15249243 Number of splices: GT/AG | 15997397 Number of splices: GC/AG | 177309 Number of splices: AT/AC | 11750 Number of splices: Non-canonical | 110576 Mismatch rate per base, % | 0.34% Deletion rate per base | 0.02% Deletion average length | 1.77 Insertion rate per base | 0.01% Insertion average length | 2.12 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 8304528 % of reads mapped to multiple loci | 16.05% Number of reads mapped to too many loci | 9246 % of reads mapped to too many loci | 0.02% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.40% % of reads unmapped: other | 0.57% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2061189 2061189 2061189 N_multimapping 8304528 8304528 8304528 N_noFeature 9511196 38031567 12045832 N_ambiguous 1208371 51276 363226 UnstrandedReadsAssigned:30666433 PositiveStrandReadsAssigned:3303157 NegativeStrandReadsAssigned:28976942 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006610 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006610-trimmed-pair1.fastq ERR11006610-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 51,751,717 reads, 30,453,788 reads pseudoaligned [quant] estimated average fragment length: 272.239 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,097 rounds 52973 ERR11006610.ke.tsv 35125 ERR11006610.se.tsv 88098 total ==> ERR11006610.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 665.165 0 0 PNS24247 1044 772.761 30.8452 1.36719 PNS24249 1928 1656.76 231.616 4.78844 PNS24246 1044 772.761 30.8452 1.36719 PNS24248 1044 772.761 30.8452 1.36719 PNS24244 1471 1199.76 101.849 2.90768 PNS24243 293 52.0022 1 0.658664 KQK14069 1603 1331.76 1305.15 33.5675 KQK14071 474 205.285 10.2763 1.7146 ==> ERR11006610.se.tsv <== BRADI_1g14170v3 1413 BRADI_1g53295v3 835 BRADI_1g59795v3 324 BRADI_1g07683v3 0 BRADI_1g00485v3 1 BRADI_1g20270v3 529 BRADI_1g74790v3 601 BRADI_1g09890v3 2 BRADI_1g77505v3 123 BRADI_1g48960v3 0 ERR11006610 completed mapping pipeline successfully