Starting /dee2/code/volunteer_pipeline.sh ERR11006611 current disk space = 1549616193536 free memory = 1602828976 ERR11006611 SRAfilesize 514381e9921287fe9e53e3e8203559f7 ERR11006611.sra ERR11006611.sra file validated ERR11006611 is paired end ERR11006611 is conventional basespace ERR11006611 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006611_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2465 37.0 37.0 37.0 37.0 37.0 2 36.246 37.0 37.0 37.0 37.0 37.0 3 36.3435 37.0 37.0 37.0 37.0 37.0 4 36.395 37.0 37.0 37.0 37.0 37.0 5 36.4685 37.0 37.0 37.0 37.0 37.0 6 36.5215 37.0 37.0 37.0 37.0 37.0 7 36.309 37.0 37.0 37.0 37.0 37.0 8 36.544 37.0 37.0 37.0 37.0 37.0 9 36.357 37.0 37.0 37.0 37.0 37.0 10-14 36.528200000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.499 37.0 37.0 37.0 37.0 37.0 20-24 36.3975 37.0 37.0 37.0 37.0 37.0 25-29 36.3904 37.0 37.0 37.0 37.0 37.0 30-34 36.3138 37.0 37.0 37.0 37.0 37.0 35-39 36.2857 37.0 37.0 37.0 37.0 37.0 40-44 36.3114 37.0 37.0 37.0 37.0 37.0 45-49 36.2596 37.0 37.0 37.0 37.0 37.0 50-54 36.1811 37.0 37.0 37.0 37.0 37.0 55-59 36.15 37.0 37.0 37.0 37.0 37.0 60-64 36.1577 37.0 37.0 37.0 37.0 37.0 65-69 36.1683 37.0 37.0 37.0 37.0 37.0 70-74 36.1464 37.0 37.0 37.0 37.0 37.0 75-79 36.1554 37.0 37.0 37.0 37.0 37.0 80-84 36.0389 37.0 37.0 37.0 37.0 37.0 85-89 36.0207 37.0 37.0 37.0 37.0 37.0 90-94 35.960899999999995 37.0 37.0 37.0 37.0 37.0 95-99 36.0241 37.0 37.0 37.0 37.0 37.0 100-104 35.9528 37.0 37.0 37.0 37.0 37.0 105-109 35.874399999999994 37.0 37.0 37.0 37.0 37.0 110-114 35.7988 37.0 37.0 37.0 37.0 37.0 115-119 35.779199999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.75019999999999 37.0 37.0 37.0 37.0 37.0 125-129 35.7158 37.0 37.0 37.0 37.0 37.0 130-134 35.4947 37.0 37.0 37.0 37.0 37.0 135-139 35.495599999999996 37.0 37.0 37.0 37.0 37.0 140-144 35.3495 37.0 37.0 37.0 34.6 37.0 145-149 35.4478 37.0 37.0 37.0 34.6 37.0 150 35.4075 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 2.0 22 0.0 23 1.0 24 2.0 25 1.0 26 8.0 27 16.0 28 22.0 29 30.0 30 44.0 31 73.0 32 81.0 33 117.0 34 146.0 35 275.0 36 2734.0 37 448.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.025 13.350000000000001 16.1 32.525 2 28.725 10.475 26.325 34.475 3 22.45 13.600000000000001 27.650000000000002 36.3 4 28.050000000000004 16.825000000000003 23.95 31.175000000000004 5 27.575 19.35 25.95 27.125 6 23.549999999999997 30.775000000000002 20.424999999999997 25.25 7 17.925 30.075000000000003 33.625 18.375 8 15.775 31.125000000000004 32.775 20.325 9 16.950000000000003 27.775 37.05 18.224999999999998 10-14 19.685 32.17 26.455000000000002 21.69 15-19 20.915 33.78 24.05 21.255 20-24 18.935 29.709999999999997 27.950000000000003 23.405 25-29 21.73 31.455 25.119999999999997 21.695 30-34 24.19 30.605 24.725 20.48 35-39 22.38 32.025 24.5 21.095 40-44 20.345 30.305 26.075 23.275000000000002 45-49 20.32 29.475 27.445000000000004 22.759999999999998 50-54 20.65 32.945 25.305 21.099999999999998 55-59 22.495 30.19 22.919999999999998 24.395 60-64 20.485 31.290000000000003 25.22 23.005 65-69 21.285 29.45 25.480000000000004 23.785 70-74 23.119999999999997 30.255 21.615000000000002 25.009999999999998 75-79 22.64 30.035 24.81 22.515 80-84 22.89 29.799999999999997 23.755000000000003 23.555 85-89 23.830000000000002 29.744999999999997 24.92 21.505 90-94 20.86 29.82 26.005 23.315 95-99 23.98 29.49 23.189999999999998 23.34 100-104 22.025 30.285 24.560000000000002 23.13 105-109 21.705 29.13 25.895000000000003 23.27 110-114 23.055 29.080000000000002 24.59 23.275000000000002 115-119 20.200000000000003 31.009999999999998 25.14 23.65 120-124 19.830000000000002 29.615000000000002 25.5 25.055 125-129 20.68 31.6 21.915000000000003 25.805 130-134 22.61 31.09 24.695 21.605 135-139 23.755000000000003 30.2 22.64 23.405 140-144 24.779999999999998 29.659999999999997 24.975 20.585 145-149 23.285 31.045 24.135 21.535 150 23.75 25.424999999999997 27.900000000000002 22.925 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 1.5 4 1.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 0.0 20 0.0 21 0.0 22 0.0 23 1.0 24 3.5 25 3.5 26 2.5 27 4.5 28 9.0 29 12.0 30 12.0 31 14.5 32 27.5 33 42.5 34 44.5 35 51.0 36 79.5 37 161.0 38 290.0 39 292.5 40 259.5 41 289.5 42 267.5 43 234.5 44 206.0 45 205.0 46 213.5 47 187.5 48 133.5 49 87.0 50 70.5 51 65.0 52 55.0 53 42.0 54 35.5 55 31.0 56 27.0 57 23.5 58 22.0 59 29.5 60 36.0 61 33.0 62 27.0 63 26.5 64 43.5 65 62.5 66 47.5 67 23.5 68 16.5 69 16.0 70 19.0 71 18.5 72 13.0 73 12.5 74 14.5 75 9.0 76 8.0 77 9.5 78 5.5 79 4.0 80 5.5 81 3.5 82 1.0 83 0.0 84 0.0 85 1.0 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.3 #Duplication Level Percentage of deduplicated Percentage of total 1 80.01579778830964 50.64999999999999 2 12.322274881516588 15.6 3 3.15955766192733 6.0 4 1.2243285939968405 3.1 5 0.5529225908372828 1.7500000000000002 6 0.5924170616113744 2.25 7 0.23696682464454977 1.05 8 0.3949447077409162 2.0 9 0.1974723538704581 1.125 >10 1.263823064770932 13.100000000000001 >50 0.0 0.0 >100 0.03949447077409163 3.375 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 135 3.375 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 36 0.8999999999999999 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 33 0.8250000000000001 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 32 0.8 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 27 0.675 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 25 0.625 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 25 0.625 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 24 0.6 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 19 0.475 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 18 0.44999999999999996 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 17 0.42500000000000004 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 16 0.4 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 15 0.375 No Hit CCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATA 14 0.35000000000000003 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 14 0.35000000000000003 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 14 0.35000000000000003 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 13 0.325 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 13 0.325 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 12 0.3 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 12 0.3 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 12 0.3 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 12 0.3 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 12 0.3 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 12 0.3 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 12 0.3 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 11 0.27499999999999997 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 11 0.27499999999999997 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 11 0.27499999999999997 No Hit ATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATA 11 0.27499999999999997 No Hit AGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGAT 11 0.27499999999999997 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 10 0.25 No Hit GCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAGCTGCAA 10 0.25 No Hit GGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGG 10 0.25 No Hit GACCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGG 9 0.22499999999999998 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 9 0.22499999999999998 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 9 0.22499999999999998 No Hit ACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGC 9 0.22499999999999998 No Hit CACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTAA 9 0.22499999999999998 No Hit ACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCAT 8 0.2 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 8 0.2 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 8 0.2 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 8 0.2 No Hit CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTAT 8 0.2 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 8 0.2 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 8 0.2 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 8 0.2 No Hit GCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGAC 8 0.2 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 8 0.2 No Hit GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG 7 0.17500000000000002 No Hit GGAGGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA 7 0.17500000000000002 No Hit TCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGT 7 0.17500000000000002 No Hit ACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAG 7 0.17500000000000002 No Hit GAGCTGAATATGCAACAGCAATCCAAGGGCGCATACCCAAACGGAAACTA 7 0.17500000000000002 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 7 0.17500000000000002 No Hit CCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGT 6 0.15 No Hit GGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACAA 6 0.15 No Hit GTCGCAGCTGCAACAGGAGCTGAATATGCAACAGCAATCCAAGGGCGCAT 6 0.15 No Hit TTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATT 6 0.15 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 6 0.15 No Hit TCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGT 6 0.15 No Hit CCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAAT 6 0.15 No Hit TGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCT 6 0.15 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 6 0.15 No Hit TCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATC 6 0.15 No Hit GTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAG 6 0.15 No Hit CACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCG 6 0.15 No Hit GGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 6 0.15 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 6 0.15 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 6 0.15 No Hit GGGCGCATACCCAAACGGAAACTAAGTTCCCACTCACGACCCATATAACA 5 0.125 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 5 0.125 No Hit CAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATT 5 0.125 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 5 0.125 No Hit CCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAG 5 0.125 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 5 0.125 No Hit GGCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGAT 5 0.125 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 5 0.125 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 5 0.125 No Hit CATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAATAAATACAGAA 5 0.125 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 5 0.125 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 5 0.125 No Hit AGGCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGA 5 0.125 No Hit CACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0125 0.0 0.0 0.0 0.0 92-93 0.025 0.0 0.0 0.0 0.0 94-95 0.025 0.0 0.0 0.0 0.0 96-97 0.025 0.0 0.0 0.0 0.0 98-99 0.025 0.0 0.0 0.0 0.0 100-101 0.025 0.0 0.0 0.0 0.0 102-103 0.025 0.0 0.0 0.0 0.0 104-105 0.025 0.0 0.0 0.0 0.0 106-107 0.05 0.0 0.0 0.0 0.0 108-109 0.05 0.0 0.0 0.0 0.0 110-111 0.05 0.0 0.0 0.0 0.0 112-113 0.075 0.0 0.0 0.0 0.0 114-115 0.075 0.0 0.0 0.0 0.0 116-117 0.075 0.0 0.0 0.0 0.0 118-119 0.0875 0.0 0.0 0.0 0.0 120-121 0.125 0.0 0.0 0.0 0.0 122-123 0.125 0.0 0.0 0.0 0.0 124-125 0.1375 0.0 0.0 0.0 0.0 126-127 0.15 0.0 0.0 0.0 0.0 128-129 0.15 0.0 0.0 0.0 0.0 130-131 0.175 0.0 0.0 0.0 0.0 132-133 0.2 0.0 0.0 0.0 0.0 134-135 0.25 0.0 0.0 0.0 0.0 136-137 0.275 0.0 0.0 0.0 0.0 138 0.3 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTTCT 30 0.0018473949 72.0 6 TCTTTTC 30 0.0018473949 72.0 5 GCTTTCT 30 0.0018473949 72.0 1 CTTTCTT 30 0.0018473949 72.0 2 TTTTCTT 35 0.0034045284 61.714283 7 TTTCTTC 40 0.005777437 54.0 8 TTCTTCA 45 0.009205684 48.0 9 TCTTCAA 25 5.183459E-4 28.8 10-14 AAATTCT 30 0.0015031899 24.0 6 TTCAAAA 30 0.0015031899 24.0 1 AAAATTC 30 0.0015031899 24.0 5 TAGCGGA 30 0.0015031899 23.999998 30-34 CTTCAAA 30 0.0015031899 23.999998 10-14 TATGTTA 30 0.0015031899 23.999998 25-29 ATGTTAG 30 0.0015031899 23.999998 25-29 TTAGCGG 30 0.0015031899 23.999998 30-34 TAGCGGG 35 0.0036813593 20.571428 55-59 AACCTTA 35 0.0036813593 20.571428 9 TATCCAT 35 0.0036813593 20.571428 45-49 AGCGGAA 35 0.0036813593 20.571428 1 >>END_MODULE ERR11006611 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006611_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.5965 37.0 37.0 37.0 37.0 37.0 2 36.255 37.0 37.0 37.0 37.0 37.0 3 36.275 37.0 37.0 37.0 37.0 37.0 4 36.174 37.0 37.0 37.0 37.0 37.0 5 36.301 37.0 37.0 37.0 37.0 37.0 6 36.3295 37.0 37.0 37.0 37.0 37.0 7 36.2855 37.0 37.0 37.0 37.0 37.0 8 36.4 37.0 37.0 37.0 37.0 37.0 9 36.2615 37.0 37.0 37.0 37.0 37.0 10-14 36.273799999999994 37.0 37.0 37.0 37.0 37.0 15-19 36.279700000000005 37.0 37.0 37.0 37.0 37.0 20-24 36.244800000000005 37.0 37.0 37.0 37.0 37.0 25-29 36.162099999999995 37.0 37.0 37.0 37.0 37.0 30-34 36.1274 37.0 37.0 37.0 37.0 37.0 35-39 36.1204 37.0 37.0 37.0 37.0 37.0 40-44 36.109399999999994 37.0 37.0 37.0 37.0 37.0 45-49 36.1065 37.0 37.0 37.0 37.0 37.0 50-54 35.9952 37.0 37.0 37.0 37.0 37.0 55-59 36.0317 37.0 37.0 37.0 37.0 37.0 60-64 35.8735 37.0 37.0 37.0 37.0 37.0 65-69 35.873400000000004 37.0 37.0 37.0 37.0 37.0 70-74 35.8677 37.0 37.0 37.0 37.0 37.0 75-79 35.8389 37.0 37.0 37.0 37.0 37.0 80-84 35.7471 37.0 37.0 37.0 37.0 37.0 85-89 35.6453 37.0 37.0 37.0 37.0 37.0 90-94 35.693400000000004 37.0 37.0 37.0 37.0 37.0 95-99 35.561099999999996 37.0 37.0 37.0 37.0 37.0 100-104 35.564299999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.4412 37.0 37.0 37.0 37.0 37.0 110-114 35.4415 37.0 37.0 37.0 37.0 37.0 115-119 35.3564 37.0 37.0 37.0 34.6 37.0 120-124 35.190799999999996 37.0 37.0 37.0 29.8 37.0 125-129 35.355999999999995 37.0 37.0 37.0 34.6 37.0 130-134 35.11409999999999 37.0 37.0 37.0 27.4 37.0 135-139 35.0667 37.0 37.0 37.0 25.0 37.0 140-144 34.9823 37.0 37.0 37.0 25.0 37.0 145-149 35.0109 37.0 37.0 37.0 25.0 37.0 150 34.734 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 1.0 19 0.0 20 1.0 21 0.0 22 3.0 23 3.0 24 6.0 25 15.0 26 11.0 27 17.0 28 28.0 29 32.0 30 39.0 31 74.0 32 85.0 33 116.0 34 212.0 35 545.0 36 2648.0 37 163.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.2730514518594 24.859908303616912 18.441161487519103 28.425878757004586 2 26.1 24.15 32.5 17.25 3 21.325 26.25 33.4 19.025 4 24.025 27.400000000000002 28.925 19.650000000000002 5 23.150000000000002 29.75 28.725 18.375 6 20.549999999999997 30.875000000000004 29.025000000000002 19.55 7 18.5 20.375 41.449999999999996 19.675 8 20.849999999999998 23.849999999999998 29.675 25.624999999999996 9 23.599999999999998 20.349999999999998 34.1 21.95 10-14 23.87 25.485000000000003 29.330000000000002 21.315 15-19 24.545 24.315 30.154999999999998 20.985 20-24 24.945 24.07 29.89 21.095 25-29 24.725 23.735 29.925 21.615000000000002 30-34 24.625 23.61 30.73 21.035 35-39 24.51 23.595 29.845 22.05 40-44 23.03 25.035 30.09 21.845 45-49 23.330000000000002 26.68 28.645 21.345 50-54 23.555 25.83 28.634999999999998 21.98 55-59 23.27 24.485 29.785 22.46 60-64 23.13 24.545 29.89 22.435 65-69 24.23 23.61 29.985 22.175 70-74 24.695 24.72 29.84 20.745 75-79 24.775 24.355 29.849999999999998 21.02 80-84 24.474999999999998 24.805 29.154999999999998 21.565 85-89 25.05 24.63 27.93 22.39 90-94 23.16 24.965 28.965000000000003 22.91 95-99 23.705000000000002 23.87 29.904999999999998 22.52 100-104 24.18 24.335 29.7 21.785 105-109 25.575 22.85 29.695 21.88 110-114 23.380000000000003 23.865 30.3 22.455 115-119 23.61 25.19 29.15 22.05 120-124 24.224999999999998 24.815 28.92 22.040000000000003 125-129 23.04 24.89 29.935000000000002 22.134999999999998 130-134 22.95 25.835 28.825 22.39 135-139 23.64 25.264999999999997 30.055 21.04 140-144 23.169999999999998 25.135 30.145 21.55 145-149 22.735 24.285 31.069999999999997 21.91 150 23.775 22.8 30.925000000000004 22.5 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0 20 1.0 21 0.0 22 1.5 23 3.5 24 5.0 25 6.5 26 7.5 27 12.5 28 12.5 29 14.5 30 25.5 31 28.5 32 27.0 33 30.0 34 45.5 35 64.0 36 113.0 37 201.0 38 232.5 39 218.0 40 233.5 41 250.0 42 237.5 43 237.0 44 245.5 45 221.5 46 188.0 47 149.0 48 117.0 49 92.0 50 63.5 51 56.0 52 45.5 53 38.5 54 40.5 55 31.0 56 25.0 57 33.0 58 39.5 59 37.5 60 35.0 61 36.0 62 33.5 63 50.0 64 65.5 65 48.0 66 34.0 67 31.0 68 28.0 69 28.5 70 28.5 71 22.0 72 16.5 73 15.5 74 20.5 75 19.0 76 13.0 77 12.0 78 9.0 79 4.0 80 2.5 81 3.0 82 3.5 83 3.0 84 1.5 85 0.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.8499999999999999 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.60000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 80.06756756756756 53.325 2 11.14864864864865 14.85 3 3.4909909909909906 6.9750000000000005 4 1.7642642642642643 4.7 5 0.975975975975976 3.25 6 0.6381381381381381 2.55 7 0.45045045045045046 2.1 8 0.3003003003003003 1.6 9 0.22522522522522523 1.35 >10 0.9009009009009009 8.025 >50 0.03753753753753754 1.275 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 51 1.275 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 24 0.6 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 23 0.575 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 22 0.5499999999999999 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 16 0.4 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 16 0.4 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 15 0.375 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 15 0.375 No Hit GTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTT 14 0.35000000000000003 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 14 0.35000000000000003 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 14 0.35000000000000003 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 13 0.325 No Hit AACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGG 12 0.3 No Hit CTGGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCACAACATCCTT 11 0.27499999999999997 No Hit CGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTG 11 0.27499999999999997 No Hit TATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTT 11 0.27499999999999997 No Hit CTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGG 10 0.25 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 10 0.25 No Hit ATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATT 10 0.25 No Hit AGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT 10 0.25 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 10 0.25 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 10 0.25 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 10 0.25 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 10 0.25 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 10 0.25 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 9 0.22499999999999998 No Hit CCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTT 9 0.22499999999999998 No Hit TATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACC 9 0.22499999999999998 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 9 0.22499999999999998 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 9 0.22499999999999998 No Hit TCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTG 9 0.22499999999999998 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 8 0.2 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 8 0.2 No Hit CCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGA 8 0.2 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 8 0.2 No Hit CTAGCACTGAAAATCGTCTTTACATCGGATGGTTCGGTGTTTTGATGATC 8 0.2 No Hit GTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTA 8 0.2 No Hit TTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGA 8 0.2 No Hit CTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTC 8 0.2 No Hit CTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGAT 7 0.17500000000000002 No Hit AGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAA 7 0.17500000000000002 No Hit GGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTC 7 0.17500000000000002 No Hit TGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATA 7 0.17500000000000002 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 7 0.17500000000000002 No Hit CTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGAT 7 0.17500000000000002 No Hit GTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCAT 7 0.17500000000000002 No Hit CTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAG 7 0.17500000000000002 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 7 0.17500000000000002 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 7 0.17500000000000002 No Hit AGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACT 7 0.17500000000000002 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 7 0.17500000000000002 No Hit GGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGTCTTTACATCGG 6 0.15 No Hit GAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGAC 6 0.15 No Hit CTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAATGGTTATACAATG 6 0.15 No Hit GCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTCGTG 6 0.15 No Hit TGATTGTATTCCAGGCAGAGCACAACATCCTTATGCATCCATTTCACATG 6 0.15 No Hit TATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACA 6 0.15 No Hit CGTCTTTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGAC 6 0.15 No Hit TGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGA 6 0.15 No Hit TCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGA 6 0.15 No Hit GCTTGTTATATGGGTCGTGAGTGGGAACTTAGTTTCCGTTTGGGTATGCG 6 0.15 No Hit GGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTT 6 0.15 No Hit AATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAATATGC 6 0.15 No Hit ATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTT 6 0.15 No Hit GGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCATAT 6 0.15 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 6 0.15 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 6 0.15 No Hit AGTAGATATTGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATG 6 0.15 No Hit GCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTTTATG 5 0.125 No Hit CTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTC 5 0.125 No Hit GGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAG 5 0.125 No Hit CCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCG 5 0.125 No Hit TATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAAT 5 0.125 No Hit TTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAAT 5 0.125 No Hit AATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCT 5 0.125 No Hit GCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGACTTATAATATTGT 5 0.125 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 5 0.125 No Hit ATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGA 5 0.125 No Hit ATTTATTATCGCCTTCATCGCAGCCCCTCCAGTAGATATTGATGGTATTC 5 0.125 No Hit GCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACG 5 0.125 No Hit CAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAATCGT 5 0.125 No Hit ATTAGTACTATGGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGT 5 0.125 No Hit CTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGG 5 0.125 No Hit GAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCG 5 0.125 No Hit TGATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATA 5 0.125 No Hit GCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGC 5 0.125 No Hit GTTTCTGGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTAT 5 0.125 No Hit CTGGTTCTTTACTTTATGGAAACAATATTATCTCTGGTGCTATTATTCCT 5 0.125 No Hit TCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAACT 5 0.125 No Hit GGTCAAGGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTT 5 0.125 No Hit CTAGTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTAC 5 0.125 No Hit AATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTA 5 0.125 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 5 0.125 No Hit ATTATCTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0125 0.0 0.0 0.0 0.0 92-93 0.025 0.0 0.0 0.0 0.0 94-95 0.025 0.0 0.0 0.0 0.0 96-97 0.025 0.0 0.0 0.0 0.0 98-99 0.025 0.0 0.0 0.0 0.0 100-101 0.025 0.0 0.0 0.0 0.0 102-103 0.025 0.0 0.0 0.0 0.0 104-105 0.025 0.0 0.0 0.0 0.0 106-107 0.025 0.0 0.0 0.0 0.0 108-109 0.025 0.0 0.0 0.0 0.0 110-111 0.025 0.0 0.0 0.0 0.0 112-113 0.05 0.0 0.0 0.0 0.0 114-115 0.05 0.0 0.0 0.0 0.0 116-117 0.05 0.0 0.0 0.0 0.0 118-119 0.0625 0.0 0.0 0.0 0.0 120-121 0.1 0.0 0.0 0.0 0.0 122-123 0.1 0.0 0.0 0.0 0.0 124-125 0.1125 0.0 0.0 0.0 0.0 126-127 0.125 0.0 0.0 0.0 0.0 128-129 0.125 0.0 0.0 0.0 0.0 130-131 0.15 0.0 0.0 0.0 0.0 132-133 0.175 0.0 0.0 0.0 0.0 134-135 0.225 0.0 0.0 0.0 0.0 136-137 0.2375 0.0 0.0 0.0 0.0 138 0.25 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGGGGG 20 0.006141849 28.797503 65-69 >>END_MODULE Read 2596950 spots for ERR11006611.sra Written 2596950 spots for ERR11006611.sra Read 2596950 spots for ERR11006611.sra Written 2596950 spots for ERR11006611.sra Read 2596961 spots for ERR11006611.sra Written 2596961 spots for ERR11006611.sra Read 2596950 spots for ERR11006611.sra Written 2596950 spots for ERR11006611.sra Read 2596950 spots for ERR11006611.sra Written 2596950 spots for ERR11006611.sra Read 2596950 spots for ERR11006611.sra Written 2596950 spots for ERR11006611.sra Read 2596950 spots for ERR11006611.sra Written 2596950 spots for ERR11006611.sra Read 2596950 spots for ERR11006611.sra Written 2596950 spots for ERR11006611.sra Read 2596950 spots for ERR11006611.sra Written 2596950 spots for ERR11006611.sra Read 2596950 spots for ERR11006611.sra Written 2596950 spots for ERR11006611.sra Read 2596950 spots for ERR11006611.sra Written 2596950 spots for ERR11006611.sra Read 2596950 spots for ERR11006611.sra Written 2596950 spots for ERR11006611.sra Read 2596950 spots for ERR11006611.sra Written 2596950 spots for ERR11006611.sra Read 2596950 spots for ERR11006611.sra Written 2596950 spots for ERR11006611.sra Read 2596950 spots for ERR11006611.sra Written 2596950 spots for ERR11006611.sra Read 2596950 spots for ERR11006611.sra Written 2596950 spots for ERR11006611.sra Read 2596950 spots for ERR11006611.sra Written 2596950 spots for ERR11006611.sra Read 2596950 spots for ERR11006611.sra Written 2596950 spots for ERR11006611.sra Read 2596950 spots for ERR11006611.sra Written 2596950 spots for ERR11006611.sra Read 2596950 spots for ERR11006611.sra Written 2596950 spots for ERR11006611.sra SRR ids: ['ERR11006611.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_c0x0hpye ERR11006611.sra spots: 51939011 blocks: [[1, 2596950], [2596951, 5193900], [5193901, 7790850], [7790851, 10387800], [10387801, 12984750], [12984751, 15581700], [15581701, 18178650], [18178651, 20775600], [20775601, 23372550], [23372551, 25969500], [25969501, 28566450], [28566451, 31163400], [31163401, 33760350], [33760351, 36357300], [36357301, 38954250], [38954251, 41551200], [41551201, 44148150], [44148151, 46745100], [46745101, 49342050], [49342051, 51939011]] ERR11006611 file size 19084147 ERR11006611 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006611 ERR11006611_1.fastq ERR11006611_2.fastq Input file: ERR11006611_1.fastq Paired file: ERR11006611_2.fastq trimmed: ERR11006611-trimmed-pair1.fastq, ERR11006611-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 19:55:34 2024 >> started Fri Dec 6 19:56:49 2024 >> done (74.606s) 51939011 read pairs processed; of these: 244 ( 0.00%) short read pairs filtered out after trimming by size control 1419 ( 0.00%) empty read pairs filtered out after trimming by size control 51937348 (100.00%) read pairs available; of these: 381829 ( 0.74%) trimmed read pairs available after processing 51555519 (99.26%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 10 0.00% 20 8 0.00% 21 13 0.00% 22 24 0.00% 23 10 0.00% 24 16 0.00% 25 14 0.00% 26 15 0.00% 27 14 0.00% 28 22 0.00% 29 23 0.00% 30 19 0.00% 31 68 0.00% 32 20 0.00% 33 22 0.00% 34 17 0.00% 35 31 0.00% 36 17 0.00% 37 20 0.00% 38 17 0.00% 39 31 0.00% 40 32 0.00% 41 30 0.00% 42 29 0.00% 43 28 0.00% 44 30 0.00% 45 32 0.00% 46 39 0.00% 47 43 0.00% 48 47 0.00% 49 44 0.00% 50 44 0.00% 51 64 0.00% 52 69 0.00% 53 54 0.00% 54 57 0.00% 55 71 0.00% 56 62 0.00% 57 72 0.00% 58 76 0.00% 59 84 0.00% 60 103 0.00% 61 104 0.00% 62 133 0.00% 63 149 0.00% 64 157 0.00% 65 181 0.00% 66 209 0.00% 67 212 0.00% 68 179 0.00% 69 233 0.00% 70 237 0.00% 71 253 0.00% 72 329 0.00% 73 314 0.00% 74 357 0.00% 75 393 0.00% 76 408 0.00% 77 423 0.00% 78 443 0.00% 79 485 0.00% 80 488 0.00% 81 528 0.00% 82 614 0.00% 83 620 0.00% 84 719 0.00% 85 789 0.00% 86 842 0.00% 87 896 0.00% 88 911 0.00% 89 964 0.00% 90 1054 0.00% 91 1103 0.00% 92 1203 0.00% 93 1238 0.00% 94 1266 0.00% 95 1411 0.00% 96 1470 0.00% 97 1496 0.00% 98 1735 0.00% 99 1769 0.00% 100 1828 0.00% 101 1911 0.00% 102 1996 0.00% 103 2121 0.00% 104 2202 0.00% 105 2309 0.00% 106 2587 0.00% 107 2697 0.01% 108 2795 0.01% 109 2909 0.01% 110 3141 0.01% 111 3283 0.01% 112 3586 0.01% 113 3591 0.01% 114 3818 0.01% 115 3999 0.01% 116 4216 0.01% 117 4421 0.01% 118 4372 0.01% 119 4717 0.01% 120 5060 0.01% 121 5440 0.01% 122 5778 0.01% 123 5978 0.01% 124 6102 0.01% 125 7022 0.01% 126 6408 0.01% 127 6263 0.01% 128 6747 0.01% 129 7475 0.01% 130 7823 0.02% 131 8159 0.02% 132 8827 0.02% 133 8253 0.02% 134 9048 0.02% 135 8546 0.02% 136 9317 0.02% 137 9392 0.02% 138 9875 0.02% 139 10577 0.02% 140 10965 0.02% 141 11878 0.02% 142 12208 0.02% 143 12656 0.02% 144 12933 0.02% 145 13732 0.03% 146 16087 0.03% 147 15357 0.03% 148 16393 0.03% 149 17173 0.03% 150 51555519 99.26% 51937348 reads passed initial QC criterion=sequence-density sequence-density=0.34 sequence-density-rank=1 fanout-score=0.00 fanout-score-rank=28 prefix-density=0.00 prefix-fanout=1.0 sequence=TGCGGGAACTTC criterion=fanout-score sequence-density=0.11 sequence-density-rank=8 fanout-score=22.62 fanout-score-rank=1 prefix-density=2.52 prefix-fanout=1.0 sequence=GAAGCCAGCCTAACGCGGTGCCTGCCACTATAGAATTCGTGAGGCCCAGCTTTCGCATAGGCCCCAGCAGATCCACTACGCAA criterion=sequence-density sequence-density=0.17 sequence-density-rank=1 fanout-score=64.62 fanout-score-rank=8 prefix-density=10.92 prefix-fanout=1.0 sequence=GCACTGAAAATAGTCTTTACA criterion=fanout-score sequence-density=0.07 sequence-density-rank=21 fanout-score=153.16 fanout-score-rank=1 prefix-density=10.67 prefix-fanout=1.0 sequence=ATAACTAGCACAGAAAATCGTCT ERR11006611 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 19:57:55 Started mapping on | Dec 06 19:57:55 Finished on | Dec 06 20:03:57 Mapping speed, Million of reads per hour | 516.50 Number of input reads | 51937348 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 41020927 Uniquely mapped reads % | 78.98% Average mapped length | 298.45 Number of splices: Total | 14428005 Number of splices: Annotated (sjdb) | 13493102 Number of splices: GT/AG | 14148774 Number of splices: GC/AG | 160513 Number of splices: AT/AC | 11530 Number of splices: Non-canonical | 107188 Mismatch rate per base, % | 0.31% Deletion rate per base | 0.02% Deletion average length | 1.70 Insertion rate per base | 0.01% Insertion average length | 2.13 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 9142372 % of reads mapped to multiple loci | 17.60% Number of reads mapped to too many loci | 9169 % of reads mapped to too many loci | 0.02% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.94% % of reads unmapped: other | 0.46% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1774049 1774049 1774049 N_multimapping 9142372 9142372 9142372 N_noFeature 10342135 37940615 12601269 N_ambiguous 1221985 50324 378405 UnstrandedReadsAssigned:29456807 PositiveStrandReadsAssigned:3029988 NegativeStrandReadsAssigned:28041253 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006611 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006611-trimmed-pair1.fastq ERR11006611-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 51,937,348 reads, 30,019,458 reads pseudoaligned [quant] estimated average fragment length: 279.01 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,141 rounds 52973 ERR11006611.ke.tsv 35125 ERR11006611.se.tsv 88098 total ==> ERR11006611.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 658.431 0 0 PNS24247 1044 765.99 31.0741 1.37263 PNS24249 1928 1649.99 247.279 5.07089 PNS24246 1044 765.99 31.0741 1.37263 PNS24248 1044 765.99 31.0741 1.37263 PNS24244 1471 1192.99 82.4987 2.33985 PNS24243 293 50.2889 0 0 KQK14069 1603 1324.99 1274.58 32.5486 KQK14071 474 199.154 18.4578 3.13595 ==> ERR11006611.se.tsv <== BRADI_1g14170v3 1482 BRADI_1g53295v3 374 BRADI_1g59795v3 283 BRADI_1g07683v3 0 BRADI_1g00485v3 5 BRADI_1g20270v3 479 BRADI_1g74790v3 531 BRADI_1g09890v3 0 BRADI_1g77505v3 127 BRADI_1g48960v3 0 ERR11006611 completed mapping pipeline successfully