Starting /dee2/code/volunteer_pipeline.sh ERR11006612 current disk space = 1549595271168 free memory = 1396197064 ERR11006612 SRAfilesize ada5563ba68e9318b12d4cd30ea5fc68 ERR11006612.sra ERR11006612.sra file validated ERR11006612 is paired end ERR11006612 is conventional basespace ERR11006612 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006612_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.3015 37.0 37.0 37.0 37.0 37.0 2 36.197 37.0 37.0 37.0 37.0 37.0 3 36.2955 37.0 37.0 37.0 37.0 37.0 4 36.3985 37.0 37.0 37.0 37.0 37.0 5 36.445 37.0 37.0 37.0 37.0 37.0 6 36.4315 37.0 37.0 37.0 37.0 37.0 7 36.277 37.0 37.0 37.0 37.0 37.0 8 36.45 37.0 37.0 37.0 37.0 37.0 9 36.32 37.0 37.0 37.0 37.0 37.0 10-14 36.4143 37.0 37.0 37.0 37.0 37.0 15-19 36.4254 37.0 37.0 37.0 37.0 37.0 20-24 36.3911 37.0 37.0 37.0 37.0 37.0 25-29 36.2695 37.0 37.0 37.0 37.0 37.0 30-34 36.2564 37.0 37.0 37.0 37.0 37.0 35-39 36.303399999999996 37.0 37.0 37.0 37.0 37.0 40-44 36.249900000000004 37.0 37.0 37.0 37.0 37.0 45-49 36.1709 37.0 37.0 37.0 37.0 37.0 50-54 36.15679999999999 37.0 37.0 37.0 37.0 37.0 55-59 36.115500000000004 37.0 37.0 37.0 37.0 37.0 60-64 36.107099999999996 37.0 37.0 37.0 37.0 37.0 65-69 36.1139 37.0 37.0 37.0 37.0 37.0 70-74 36.088800000000006 37.0 37.0 37.0 37.0 37.0 75-79 35.9674 37.0 37.0 37.0 37.0 37.0 80-84 35.956599999999995 37.0 37.0 37.0 37.0 37.0 85-89 35.9237 37.0 37.0 37.0 37.0 37.0 90-94 35.919200000000004 37.0 37.0 37.0 37.0 37.0 95-99 35.946000000000005 37.0 37.0 37.0 37.0 37.0 100-104 35.9048 37.0 37.0 37.0 37.0 37.0 105-109 35.852 37.0 37.0 37.0 37.0 37.0 110-114 35.7339 37.0 37.0 37.0 37.0 37.0 115-119 35.72579999999999 37.0 37.0 37.0 37.0 37.0 120-124 35.6883 37.0 37.0 37.0 37.0 37.0 125-129 35.583800000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.5426 37.0 37.0 37.0 37.0 37.0 135-139 35.440200000000004 37.0 37.0 37.0 34.6 37.0 140-144 35.304500000000004 37.0 37.0 37.0 32.2 37.0 145-149 35.3696 37.0 37.0 37.0 34.6 37.0 150 35.318 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 1.0 23 1.0 24 2.0 25 4.0 26 6.0 27 17.0 28 28.0 29 37.0 30 43.0 31 75.0 32 98.0 33 100.0 34 162.0 35 303.0 36 2792.0 37 330.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.224999999999994 13.225000000000001 15.075 33.475 2 30.625000000000004 12.049999999999999 25.275 32.05 3 24.85 14.374999999999998 24.9 35.875 4 29.275000000000002 15.975 23.1 31.65 5 30.975 20.424999999999997 22.650000000000002 25.95 6 27.35 27.275 20.1 25.275 7 17.4 30.099999999999998 32.125 20.375 8 17.8 31.6 29.799999999999997 20.8 9 20.200000000000003 26.875 32.175 20.75 10-14 20.84 31.795 25.509999999999998 21.855 15-19 22.975 30.185000000000002 23.825 23.015 20-24 21.15 29.125 25.465 24.26 25-29 22.5 30.275000000000002 24.675 22.55 30-34 24.235 30.669999999999998 23.06 22.035 35-39 23.175 29.815 24.765 22.245 40-44 22.33 29.439999999999998 25.290000000000003 22.939999999999998 45-49 21.945 28.92 26.064999999999998 23.07 50-54 21.945 30.259999999999998 24.925 22.869999999999997 55-59 22.470000000000002 29.225 23.345 24.959999999999997 60-64 20.435 29.744999999999997 25.885 23.935000000000002 65-69 22.439999999999998 29.744999999999997 23.855 23.96 70-74 23.335 28.645 22.59 25.430000000000003 75-79 22.775000000000002 29.154999999999998 24.990000000000002 23.080000000000002 80-84 24.195 28.305000000000003 23.875 23.625 85-89 23.244999999999997 27.71 25.314999999999998 23.73 90-94 21.83 28.389999999999997 25.25 24.529999999999998 95-99 23.73 28.655 23.150000000000002 24.465 100-104 22.335 29.54 23.325000000000003 24.8 105-109 22.720000000000002 28.63 24.545 24.104999999999997 110-114 23.78 28.875 23.425 23.919999999999998 115-119 22.24 29.794999999999998 23.265 24.7 120-124 21.325 29.445 23.895 25.335 125-129 21.875 29.65 22.45 26.025 130-134 23.66 30.095 22.925 23.32 135-139 24.715 28.4 22.585 24.3 140-144 24.52 28.535 24.72 22.225 145-149 23.53 29.770000000000003 24.04 22.66 150 24.45 26.974999999999998 24.65 23.925 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.0 21 2.0 22 1.5 23 1.5 24 2.0 25 2.0 26 3.5 27 6.0 28 6.0 29 8.0 30 15.5 31 17.5 32 23.5 33 31.0 34 39.5 35 53.0 36 74.5 37 144.5 38 234.5 39 232.5 40 221.5 41 258.0 42 247.0 43 219.0 44 204.5 45 216.0 46 211.0 47 168.5 48 135.5 49 99.5 50 75.0 51 69.5 52 55.5 53 46.0 54 42.0 55 35.0 56 32.5 57 32.5 58 32.0 59 38.5 60 37.0 61 36.0 62 42.0 63 41.0 64 71.5 65 93.5 66 57.5 67 28.0 68 31.5 69 34.0 70 27.5 71 19.5 72 20.5 73 23.5 74 20.5 75 18.5 76 14.5 77 9.5 78 6.0 79 5.5 80 5.0 81 4.5 82 4.5 83 3.0 84 2.0 85 1.5 86 0.5 87 0.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.55 #Duplication Level Percentage of deduplicated Percentage of total 1 81.3841598815692 54.974999999999994 2 12.028127313101407 16.25 3 2.4056254626202813 4.875 4 1.2213175425610658 3.3000000000000003 5 0.6661732050333087 2.25 6 0.33308660251665434 1.35 7 0.44411547002220575 2.1 8 0.1850481125092524 1.0 9 0.22205773501110287 1.35 >10 1.073279052553664 11.175 >50 0.03700962250185048 1.375 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCA 55 1.375 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 32 0.8 No Hit GCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACG 29 0.7250000000000001 No Hit CCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAA 28 0.7000000000000001 No Hit CTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATAA 26 0.65 No Hit GGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAA 23 0.575 No Hit GGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGA 18 0.44999999999999996 No Hit GGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGC 18 0.44999999999999996 No Hit GCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAG 17 0.42500000000000004 No Hit CGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTA 16 0.4 No Hit GGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGC 16 0.4 No Hit CGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCC 16 0.4 No Hit AGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 15 0.375 No Hit GCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATC 13 0.325 No Hit CGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCC 13 0.325 No Hit GAACGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAG 13 0.325 No Hit CGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGT 12 0.3 No Hit TGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 12 0.3 No Hit CTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCAT 12 0.3 No Hit TGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 12 0.3 No Hit GTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTC 11 0.27499999999999997 No Hit GGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACT 11 0.27499999999999997 No Hit TGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACA 11 0.27499999999999997 No Hit CCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGT 11 0.27499999999999997 No Hit CGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGT 11 0.27499999999999997 No Hit GGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTT 11 0.27499999999999997 No Hit GATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 10 0.25 No Hit CTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTT 10 0.25 No Hit GCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAA 10 0.25 No Hit GTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACC 10 0.25 No Hit CTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTA 9 0.22499999999999998 No Hit ACCAGATATTCCTAAAGGCATACCATCAGAGAAGCTTCCTTGACCAATAG 9 0.22499999999999998 No Hit GCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGT 9 0.22499999999999998 No Hit GCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTT 9 0.22499999999999998 No Hit GCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTCGCAGCTGCAA 9 0.22499999999999998 No Hit GACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCA 9 0.22499999999999998 No Hit CTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTG 8 0.2 No Hit GTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGA 8 0.2 No Hit GCTCGCGAATACCATCAATATCTACTGGAGGGGCTGCGATGAAGGCGATA 8 0.2 No Hit TTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAA 8 0.2 No Hit CTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAG 8 0.2 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 7 0.17500000000000002 No Hit CCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGT 7 0.17500000000000002 No Hit GCACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTC 7 0.17500000000000002 No Hit GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCA 7 0.17500000000000002 No Hit AGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAAGTAAAGAAC 7 0.17500000000000002 No Hit GCAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAA 7 0.17500000000000002 No Hit TGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGT 7 0.17500000000000002 No Hit GTTTCCATAAAGTAAAGAACCAGAAACAGGCTCGCGAATACCATCAATAT 7 0.17500000000000002 No Hit CGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAAC 7 0.17500000000000002 No Hit TGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCA 7 0.17500000000000002 No Hit GCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAA 7 0.17500000000000002 No Hit GGGGCTGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAAGGT 7 0.17500000000000002 No Hit GTGCAATCCGATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATAT 6 0.15 No Hit CTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAAC 6 0.15 No Hit ACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGG 6 0.15 No Hit AGCTCATAAGGACCACCATTGTATAACCATTCATCAACGGATGCAGCTTC 6 0.15 No Hit GGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATAAA 6 0.15 No Hit TCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGT 6 0.15 No Hit CCATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGT 6 0.15 No Hit GTGCTCTGCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTA 6 0.15 No Hit GTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAG 6 0.15 No Hit TCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCG 5 0.125 No Hit CGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTAC 5 0.125 No Hit GGCACCTCGGCCGTGCTGCGGCTGACGCACCGTCACGGGCCGTGCGCCGG 5 0.125 No Hit AATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTC 5 0.125 No Hit GCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCA 5 0.125 No Hit CCACCATTGTATAACCATTCATCAACGGATGCAGCTTCCCAAATTGGGTA 5 0.125 No Hit TGGATGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGA 5 0.125 No Hit CATATAACAAGCTACACCAAGTAAGAAGTGTAGAACAATTAGCTCATAAG 5 0.125 No Hit CATCAGAGAAGCTTCCTTGACCAATAGGGTAAATCAAGAAAACAGCAGTC 5 0.125 No Hit CCCACTCACGACCCATATAACAAGCTACACCAAGTAAGAAGTGTAGAACA 5 0.125 No Hit GTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCA 5 0.125 No Hit GTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTA 5 0.125 No Hit ATTGCCGCAGAAGTAGGAATAATAGCACCAGAGATAATATTGTTTCCATA 5 0.125 No Hit CACCAGAGATAATATTGTTTCCATAAAGTAAAGAACCAGAAACAGGCTCG 5 0.125 No Hit AGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTAT 5 0.125 No Hit TCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTT 5 0.125 No Hit CCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTT 5 0.125 No Hit CACCTAACATGTGAAATGGATGCATAAGGATGTTGTGCTCTGCCTGGAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.025 0.0 0.0 0.0 0.0 100-101 0.025 0.0 0.0 0.0 0.0 102-103 0.025 0.0 0.0 0.0 0.0 104-105 0.025 0.0 0.0 0.0 0.0 106-107 0.025 0.0 0.0 0.0 0.0 108-109 0.05 0.0 0.0 0.0 0.0 110-111 0.05 0.0 0.0 0.0 0.0 112-113 0.05 0.0 0.0 0.0 0.0 114-115 0.05 0.0 0.0 0.0 0.0 116-117 0.05 0.0 0.0 0.0 0.0 118-119 0.05 0.0 0.0 0.0 0.0 120-121 0.1 0.0 0.0 0.0 0.0 122-123 0.175 0.0 0.0 0.0 0.0 124-125 0.225 0.0 0.0 0.0 0.0 126-127 0.35 0.0 0.0 0.0 0.0 128-129 0.4125 0.0 0.0 0.0 0.0 130-131 0.5375 0.0 0.0 0.0 0.0 132-133 0.6 0.0 0.0 0.0 0.0 134-135 0.6 0.0 0.0 0.0 0.0 136-137 0.6375 0.0 0.0 0.0 0.0 138 0.75 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACGGGC 10 0.006973645 144.0 8 GCGGGAA 30 0.0018473949 72.0 1 CTTCAAG 35 0.0034045284 61.714283 8 TTCAAGA 35 0.0034045284 61.714283 9 GAACTTC 35 0.0034045284 61.714283 5 CGGGAAC 35 0.0034045284 61.714283 2 GGAACTT 35 0.0034045284 61.714283 4 ACTTCAA 40 0.005777437 54.0 7 AACTTCA 40 0.005777437 54.0 6 GGGAACT 40 0.005777437 54.0 3 CCCCCCC 20 0.006139246 28.8 20-24 ACCAAGA 40 0.007966741 18.0 65-69 AAGAACC 40 0.007966741 18.0 50-54 >>END_MODULE ERR11006612 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR11006612_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.409 37.0 37.0 37.0 37.0 37.0 2 35.945 37.0 37.0 37.0 37.0 37.0 3 36.1785 37.0 37.0 37.0 37.0 37.0 4 36.166 37.0 37.0 37.0 37.0 37.0 5 36.296 37.0 37.0 37.0 37.0 37.0 6 36.09 37.0 37.0 37.0 37.0 37.0 7 36.133 37.0 37.0 37.0 37.0 37.0 8 36.3275 37.0 37.0 37.0 37.0 37.0 9 36.289 37.0 37.0 37.0 37.0 37.0 10-14 36.2247 37.0 37.0 37.0 37.0 37.0 15-19 36.2245 37.0 37.0 37.0 37.0 37.0 20-24 36.15260000000001 37.0 37.0 37.0 37.0 37.0 25-29 36.144 37.0 37.0 37.0 37.0 37.0 30-34 36.0749 37.0 37.0 37.0 37.0 37.0 35-39 36.1357 37.0 37.0 37.0 37.0 37.0 40-44 36.0624 37.0 37.0 37.0 37.0 37.0 45-49 35.9966 37.0 37.0 37.0 37.0 37.0 50-54 35.9674 37.0 37.0 37.0 37.0 37.0 55-59 35.8629 37.0 37.0 37.0 37.0 37.0 60-64 35.8404 37.0 37.0 37.0 37.0 37.0 65-69 35.8887 37.0 37.0 37.0 37.0 37.0 70-74 35.784400000000005 37.0 37.0 37.0 37.0 37.0 75-79 35.8067 37.0 37.0 37.0 37.0 37.0 80-84 35.725199999999994 37.0 37.0 37.0 37.0 37.0 85-89 35.6973 37.0 37.0 37.0 37.0 37.0 90-94 35.7114 37.0 37.0 37.0 37.0 37.0 95-99 35.638 37.0 37.0 37.0 37.0 37.0 100-104 35.523 37.0 37.0 37.0 37.0 37.0 105-109 35.41009999999999 37.0 37.0 37.0 37.0 37.0 110-114 35.4387 37.0 37.0 37.0 37.0 37.0 115-119 35.309 37.0 37.0 37.0 32.2 37.0 120-124 35.1819 37.0 37.0 37.0 27.4 37.0 125-129 35.324 37.0 37.0 37.0 34.6 37.0 130-134 35.2398 37.0 37.0 37.0 27.4 37.0 135-139 35.0457 37.0 37.0 37.0 25.0 37.0 140-144 34.9536 37.0 37.0 37.0 25.0 37.0 145-149 35.057300000000005 37.0 37.0 37.0 25.0 37.0 150 34.8595 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 1.0 20 1.0 21 1.0 22 3.0 23 6.0 24 8.0 25 8.0 26 13.0 27 16.0 28 17.0 29 42.0 30 60.0 31 55.0 32 83.0 33 129.0 34 219.0 35 597.0 36 2589.0 37 151.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 31.625891946992862 24.184505606523953 16.87054026503568 27.3190621814475 2 28.275 23.575 29.875 18.275 3 22.625 27.05 29.349999999999998 20.974999999999998 4 25.424999999999997 26.375 27.075 21.125 5 24.85 29.475 26.325 19.35 6 23.150000000000002 31.025000000000002 25.874999999999996 19.950000000000003 7 21.575 21.349999999999998 38.2 18.875 8 22.275 23.375 29.275000000000002 25.074999999999996 9 23.525 20.225 33.0 23.25 10-14 24.14 25.785000000000004 27.065 23.01 15-19 24.5 24.495 29.435 21.57 20-24 24.375 24.654999999999998 29.310000000000002 21.66 25-29 24.404999999999998 24.47 29.24 21.884999999999998 30-34 24.955 23.945 29.98 21.12 35-39 25.580000000000002 23.425 29.205 21.790000000000003 40-44 24.095 24.44 28.98 22.485 45-49 24.605 24.5 29.09 21.805 50-54 24.665 24.145 28.265 22.925 55-59 24.5 24.404999999999998 27.965 23.13 60-64 24.03 23.71 29.459999999999997 22.8 65-69 24.815 24.265 28.23 22.689999999999998 70-74 25.074999999999996 24.2 28.305000000000003 22.42 75-79 24.82 23.625 29.565 21.990000000000002 80-84 25.415 23.595 29.025000000000002 21.965 85-89 25.419999999999998 23.990000000000002 27.474999999999998 23.115 90-94 24.11 23.76 28.22 23.91 95-99 23.72 23.52 29.705 23.055 100-104 24.505 23.72 29.64 22.134999999999998 105-109 25.635 23.06 29.075 22.23 110-114 24.255 24.015 29.18 22.55 115-119 24.635 24.66 28.74 21.965 120-124 24.104999999999997 24.805 28.165000000000003 22.925 125-129 23.5 24.69 28.845 22.965 130-134 23.53 25.185000000000002 28.26 23.025000000000002 135-139 24.490000000000002 24.29 29.165000000000003 22.055 140-144 24.13 23.715 29.28 22.875 145-149 23.549999999999997 23.815 29.74 22.895 150 23.849999999999998 23.375 29.075 23.7 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 0.0 22 0.5 23 1.5 24 3.0 25 3.5 26 5.0 27 11.5 28 15.5 29 17.5 30 22.0 31 27.0 32 29.5 33 32.5 34 40.5 35 61.5 36 104.0 37 164.0 38 192.5 39 193.0 40 216.0 41 234.5 42 210.5 43 192.0 44 221.5 45 240.0 46 216.0 47 158.5 48 112.5 49 91.5 50 75.5 51 63.5 52 49.5 53 44.0 54 35.5 55 32.5 56 44.0 57 42.5 58 38.5 59 38.5 60 41.0 61 43.0 62 38.5 63 60.5 64 78.0 65 63.0 66 55.5 67 47.0 68 40.5 69 38.5 70 35.5 71 34.0 72 29.5 73 21.5 74 17.0 75 15.0 76 9.0 77 9.0 78 9.0 79 9.0 80 6.5 81 5.0 82 5.0 83 3.5 84 2.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.9 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.675 #Duplication Level Percentage of deduplicated Percentage of total 1 81.21683763707111 57.4 2 11.213300318358684 15.85 3 3.25433321542271 6.9 4 1.8747789175804739 5.3 5 0.6720905553590378 2.375 6 0.3537318712415989 1.5 7 0.4244782454899187 2.1 8 0.17686593562079944 1.0 9 0.21223912274495935 1.35 >10 0.6013441811107181 6.225 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAA 36 0.8999999999999999 No Hit CTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGG 21 0.525 No Hit AGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGT 20 0.5 No Hit CAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCG 19 0.475 No Hit AGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCA 15 0.375 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 14 0.35000000000000003 No Hit CTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAG 13 0.325 No Hit CAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCT 12 0.3 No Hit TATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAA 12 0.3 No Hit GCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTT 12 0.3 No Hit GAGCACAACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGT 12 0.3 No Hit CCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCC 11 0.27499999999999997 No Hit CAAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTT 11 0.27499999999999997 No Hit ATTCAGTGCTATGCATGGTTCCTTGGTAACCTCTAGTTTGATCAGGGAAA 11 0.27499999999999997 No Hit AAATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTA 10 0.25 No Hit CTATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAA 10 0.25 No Hit GGAAGCTTCTCTGATGGTATGCCTTTAGGAATATCTGGTACTTTCAACTT 10 0.25 No Hit CCGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTA 9 0.22499999999999998 No Hit GTTATTTTGGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGT 9 0.22499999999999998 No Hit GGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTT 9 0.22499999999999998 No Hit ATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGG 9 0.22499999999999998 No Hit GAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTA 9 0.22499999999999998 No Hit CTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTA 9 0.22499999999999998 No Hit TAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTA 8 0.2 No Hit CATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCT 8 0.2 No Hit CTTACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGG 8 0.2 No Hit TGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGAC 8 0.2 No Hit GTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGAC 8 0.2 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 7 0.17500000000000002 No Hit GTTTTCGGCGGTTCCCTATTCAGTGCTATGCATGGTTCCTTGGTAACCTC 7 0.17500000000000002 No Hit CAACTCTCGTTCTTTACACTTCTTCTTGGCTGCTTGGCCTGTAGTAGGAA 7 0.17500000000000002 No Hit GATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTC 7 0.17500000000000002 No Hit CGTTGATGAATGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTAC 7 0.17500000000000002 No Hit AGCGAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTG 7 0.17500000000000002 No Hit AATCGGATTGCACTTTTACCCAATTTGGGAAGCTGCATCCGTTGATGAAT 7 0.17500000000000002 No Hit TTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACACTTCTTCTTGGC 7 0.17500000000000002 No Hit CAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTGGTGTAG 7 0.17500000000000002 No Hit GTTAGGTGTAGCTGGTGTTTTCGGCGGTTCCCTATTCAGTGCTATGCATG 7 0.17500000000000002 No Hit AACATCCTTATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTTTTCGG 7 0.17500000000000002 No Hit GTTTGATCAGGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAA 7 0.17500000000000002 No Hit AAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATC 6 0.15 No Hit GGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTT 6 0.15 No Hit CCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGCGCTGCGGG 6 0.15 No Hit ATATTATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATA 6 0.15 No Hit GAGAGCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCG 6 0.15 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 6 0.15 No Hit CGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCAC 6 0.15 No Hit CTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTTCCAA 6 0.15 No Hit ATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTG 6 0.15 No Hit GAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGACGCGAAAGT 6 0.15 No Hit GAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAAGAGGAAGAGAC 5 0.125 No Hit GCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCA 5 0.125 No Hit GATGGTATTCGCGAGCCTGTTTCTGGTTCTTTACTTTATGGAAACAATAT 5 0.125 No Hit GGGAAACTACTGAAAATGAATCTGCTAATGAGGGTTACAAATTTGGTCAA 5 0.125 No Hit TTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAGA 5 0.125 No Hit GAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCTT 5 0.125 No Hit AATTTGGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTAT 5 0.125 No Hit TTCACCGTCACCACGTACTGGAGCGTGCCGATGGAGTGGCCGATGTTGGC 5 0.125 No Hit GTTTTGATGATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGC 5 0.125 No Hit GTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACTTG 5 0.125 No Hit AGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAATCT 5 0.125 No Hit ATTTCTAGTTAATATGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCA 5 0.125 No Hit TTCTTCTTGGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGG 5 0.125 No Hit CGCGTATCCCCCGAAAGGGAATCGGGTTAAGATTTCCCGAGCCGGGATGT 5 0.125 No Hit TGAGCTAATTGTTCTACACTTCTTACTTGGTGTAGCTTGTTATATGGGTC 5 0.125 No Hit GCTTGTAACCCGAGTGGGGGCTTTAAGGGTGGTGTGGACGTTGCCGTAGC 5 0.125 No Hit TTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAACTGGA 5 0.125 No Hit TGGTTATACAATGGTGGTCCTTATGAGCTAATTGTTCTACACTTCTTACT 5 0.125 No Hit GTACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACTGAAAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.025 0.0 0.0 0.0 0.0 100-101 0.025 0.0 0.0 0.0 0.0 102-103 0.025 0.0 0.0 0.0 0.0 104-105 0.025 0.0 0.0 0.0 0.0 106-107 0.025 0.0 0.0 0.0 0.0 108-109 0.05 0.0 0.0 0.0 0.0 110-111 0.05 0.0 0.0 0.0 0.0 112-113 0.05 0.0 0.0 0.0 0.0 114-115 0.05 0.0 0.0 0.0 0.0 116-117 0.05 0.0 0.0 0.0 0.0 118-119 0.05 0.0 0.0 0.0 0.0 120-121 0.1 0.0 0.0 0.0 0.0 122-123 0.175 0.0 0.0 0.0 0.0 124-125 0.225 0.0 0.0 0.0 0.0 126-127 0.325 0.0 0.0 0.0 0.0 128-129 0.4 0.0 0.0 0.0 0.0 130-131 0.5375 0.0 0.0 0.0 0.0 132-133 0.6 0.0 0.0 0.0 0.0 134-135 0.6 0.0 0.0 0.0 0.0 136-137 0.6375 0.0 0.0 0.0 0.0 138 0.775 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGAACAT 10 0.0069754543 143.9875 3 >>END_MODULE Read 2560445 spots for ERR11006612.sra Written 2560445 spots for ERR11006612.sra Read 2560445 spots for ERR11006612.sra Written 2560445 spots for ERR11006612.sra Read 2560445 spots for ERR11006612.sra Written 2560445 spots for ERR11006612.sra Read 2560445 spots for ERR11006612.sra Written 2560445 spots for ERR11006612.sra Read 2560445 spots for ERR11006612.sra Written 2560445 spots for ERR11006612.sra Read 2560445 spots for ERR11006612.sra Written 2560445 spots for ERR11006612.sra Read 2560445 spots for ERR11006612.sra Written 2560445 spots for ERR11006612.sra Read 2560455 spots for ERR11006612.sra Written 2560455 spots for ERR11006612.sra Read 2560445 spots for ERR11006612.sra Written 2560445 spots for ERR11006612.sra Read 2560445 spots for ERR11006612.sra Written 2560445 spots for ERR11006612.sra Read 2560445 spots for ERR11006612.sra Written 2560445 spots for ERR11006612.sra Read 2560445 spots for ERR11006612.sra Written 2560445 spots for ERR11006612.sra Read 2560445 spots for ERR11006612.sra Written 2560445 spots for ERR11006612.sra Read 2560445 spots for ERR11006612.sra Written 2560445 spots for ERR11006612.sra Read 2560445 spots for ERR11006612.sra Written 2560445 spots for ERR11006612.sra Read 2560445 spots for ERR11006612.sra Written 2560445 spots for ERR11006612.sra Read 2560445 spots for ERR11006612.sra Written 2560445 spots for ERR11006612.sra Read 2560445 spots for ERR11006612.sra Written 2560445 spots for ERR11006612.sra Read 2560445 spots for ERR11006612.sra Written 2560445 spots for ERR11006612.sra Read 2560445 spots for ERR11006612.sra Written 2560445 spots for ERR11006612.sra SRR ids: ['ERR11006612.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_rxd7eo34 ERR11006612.sra spots: 51208910 blocks: [[1, 2560445], [2560446, 5120890], [5120891, 7681335], [7681336, 10241780], [10241781, 12802225], [12802226, 15362670], [15362671, 17923115], [17923116, 20483560], [20483561, 23044005], [23044006, 25604450], [25604451, 28164895], [28164896, 30725340], [30725341, 33285785], [33285786, 35846230], [35846231, 38406675], [38406676, 40967120], [40967121, 43527565], [43527566, 46088010], [46088011, 48648455], [48648456, 51208910]] ERR11006612 file size 18815724 ERR11006612 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR11006612 ERR11006612_1.fastq ERR11006612_2.fastq Input file: ERR11006612_1.fastq Paired file: ERR11006612_2.fastq trimmed: ERR11006612-trimmed-pair1.fastq, ERR11006612-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 19:58:16 2024 >> started Fri Dec 6 19:59:45 2024 >> done (89.155s) 51208910 read pairs processed; of these: 199 ( 0.00%) short read pairs filtered out after trimming by size control 1560 ( 0.00%) empty read pairs filtered out after trimming by size control 51207151 (100.00%) read pairs available; of these: 551479 ( 1.08%) trimmed read pairs available after processing 50655672 (98.92%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 11 0.00% 19 10 0.00% 20 8 0.00% 21 14 0.00% 22 19 0.00% 23 11 0.00% 24 17 0.00% 25 59 0.00% 26 19 0.00% 27 23 0.00% 28 26 0.00% 29 24 0.00% 30 26 0.00% 31 102 0.00% 32 27 0.00% 33 27 0.00% 34 23 0.00% 35 18 0.00% 36 22 0.00% 37 27 0.00% 38 34 0.00% 39 35 0.00% 40 34 0.00% 41 27 0.00% 42 53 0.00% 43 38 0.00% 44 45 0.00% 45 44 0.00% 46 55 0.00% 47 54 0.00% 48 60 0.00% 49 71 0.00% 50 89 0.00% 51 71 0.00% 52 84 0.00% 53 91 0.00% 54 95 0.00% 55 119 0.00% 56 142 0.00% 57 130 0.00% 58 138 0.00% 59 149 0.00% 60 169 0.00% 61 203 0.00% 62 216 0.00% 63 220 0.00% 64 254 0.00% 65 289 0.00% 66 250 0.00% 67 279 0.00% 68 314 0.00% 69 319 0.00% 70 371 0.00% 71 427 0.00% 72 470 0.00% 73 463 0.00% 74 557 0.00% 75 579 0.00% 76 633 0.00% 77 663 0.00% 78 698 0.00% 79 747 0.00% 80 748 0.00% 81 869 0.00% 82 934 0.00% 83 970 0.00% 84 1048 0.00% 85 1192 0.00% 86 1308 0.00% 87 1305 0.00% 88 1339 0.00% 89 1571 0.00% 90 1572 0.00% 91 1666 0.00% 92 1783 0.00% 93 1838 0.00% 94 1930 0.00% 95 2109 0.00% 96 2185 0.00% 97 2314 0.00% 98 2404 0.00% 99 2524 0.00% 100 2651 0.01% 101 2922 0.01% 102 2885 0.01% 103 3126 0.01% 104 3282 0.01% 105 3617 0.01% 106 3571 0.01% 107 3876 0.01% 108 4106 0.01% 109 4087 0.01% 110 4543 0.01% 111 4735 0.01% 112 4897 0.01% 113 5166 0.01% 114 5348 0.01% 115 5520 0.01% 116 6032 0.01% 117 6355 0.01% 118 6411 0.01% 119 7058 0.01% 120 7199 0.01% 121 7709 0.02% 122 8088 0.02% 123 8483 0.02% 124 8684 0.02% 125 9493 0.02% 126 9150 0.02% 127 9412 0.02% 128 9634 0.02% 129 10311 0.02% 130 11058 0.02% 131 12132 0.02% 132 12384 0.02% 133 12162 0.02% 134 12711 0.02% 135 12620 0.02% 136 13776 0.03% 137 14121 0.03% 138 14738 0.03% 139 15474 0.03% 140 16283 0.03% 141 17054 0.03% 142 18035 0.04% 143 18764 0.04% 144 19266 0.04% 145 20075 0.04% 146 21455 0.04% 147 21851 0.04% 148 22951 0.04% 149 24317 0.05% 150 50655672 98.92% 51207151 reads passed initial QC criterion=sequence-density sequence-density=0.30 sequence-density-rank=1 fanout-score=1.00 fanout-score-rank=34 prefix-density=0.01 prefix-fanout=1.0 sequence=GTGGTGGTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCCCGTTATCCTTCCACCGTTGGAAGCGGGCAGTTGTCGCTGCTCTGTGAAGCCAGCCTCACGCTGTGCCTGCCAACATTATGGGCCGCGAAGCCTAGCTTTCGCTTAAGCTCCAACGGCCCACTACGCAACTTGGAACGGGCGGGCCATCAGTAGCACACCCAGACCAGGCCCGCAGCGCTACGGCAACGTCCACACCACCCTTAAAGCCCCCACT criterion=fanout-score sequence-density=0.07 sequence-density-rank=21 fanout-score=98.26 fanout-score-rank=1 prefix-density=7.11 prefix-fanout=1.0 sequence=AGCACGGTTGAGGATATCAGCCC criterion=sequence-density sequence-density=0.21 sequence-density-rank=1 fanout-score=15.15 fanout-score-rank=9 prefix-density=3.18 prefix-fanout=1.0 sequence=TTGCGTAGTGGATCTGCTGGGGCCTATGCGAAAGCTGGGCCTCACGAATTCTATAGTGGCAGGCACCGCGTTAGGCTGGCTTC criterion=fanout-score sequence-density=0.07 sequence-density-rank=13 fanout-score=104.01 fanout-score-rank=1 prefix-density=7.18 prefix-fanout=1.0 sequence=CTGGATAACTATCACTGAAAATC ERR11006612 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 20:01:03 Started mapping on | Dec 06 20:01:03 Finished on | Dec 06 20:06:57 Mapping speed, Million of reads per hour | 520.75 Number of input reads | 51207151 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 40430963 Uniquely mapped reads % | 78.96% Average mapped length | 298.27 Number of splices: Total | 16663449 Number of splices: Annotated (sjdb) | 15675082 Number of splices: GT/AG | 16378115 Number of splices: GC/AG | 181205 Number of splices: AT/AC | 14306 Number of splices: Non-canonical | 89823 Mismatch rate per base, % | 0.31% Deletion rate per base | 0.02% Deletion average length | 1.78 Insertion rate per base | 0.01% Insertion average length | 2.11 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 8681454 % of reads mapped to multiple loci | 16.95% Number of reads mapped to too many loci | 20340 % of reads mapped to too many loci | 0.04% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.12% % of reads unmapped: other | 0.93% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2094734 2094734 2094734 N_multimapping 8681454 8681454 8681454 N_noFeature 9805208 36680757 12750813 N_ambiguous 1174790 44047 350041 UnstrandedReadsAssigned:29450965 PositiveStrandReadsAssigned:3706159 NegativeStrandReadsAssigned:27330109 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR11006612 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR11006612-trimmed-pair1.fastq ERR11006612-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 51,207,151 reads, 29,616,346 reads pseudoaligned [quant] estimated average fragment length: 266.893 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,141 rounds 52973 ERR11006612.ke.tsv 35125 ERR11006612.se.tsv 88098 total ==> ERR11006612.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 670.606 0 0 PNS24247 1044 778.107 50.0998 2.38351 PNS24249 1928 1662.11 250.958 5.58937 PNS24246 1044 778.107 50.0998 2.38351 PNS24248 1044 778.107 50.0998 2.38351 PNS24244 1471 1205.11 35.7424 1.09794 PNS24243 293 54.047 0 0 KQK14069 1603 1337.11 1149.9 31.8358 KQK14071 474 210.346 45.9125 8.08011 ==> ERR11006612.se.tsv <== BRADI_1g14170v3 1284 BRADI_1g53295v3 1167 BRADI_1g59795v3 303 BRADI_1g07683v3 0 BRADI_1g00485v3 1 BRADI_1g20270v3 431 BRADI_1g74790v3 891 BRADI_1g09890v3 1 BRADI_1g77505v3 123 BRADI_1g48960v3 5 ERR11006612 completed mapping pipeline successfully