Starting /dee2/code/volunteer_pipeline.sh ERR1942992 current disk space = 1523569659904 free memory = 1601264008 ERR1942992 SRAfilesize 2e03f525066633f3c7240c4ca2ee178d ERR1942992.sra ERR1942992.sra file validated ERR1942992 is single end ERR1942992 is conventional basespace ERR1942992 read1 length is 25-260 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1942992_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 25-260 %GC 58 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.735 25.0 22.0 27.0 16.0 28.0 2 23.39975 25.0 21.0 27.0 14.0 28.0 3 23.6845 25.0 22.0 27.0 15.0 28.0 4 24.02675 25.0 22.0 27.0 14.0 28.0 5 23.1 24.0 20.0 27.0 14.0 28.0 6 23.973 25.0 22.0 27.0 14.0 29.0 7 22.8 24.0 20.0 26.0 15.0 28.0 8 24.14725 25.0 22.0 27.0 19.0 29.0 9 23.449 25.0 21.0 27.0 15.0 28.0 10-14 23.9375 24.6 22.2 27.0 17.8 28.4 15-19 23.613049999999998 24.6 21.6 27.0 15.8 28.4 20-24 23.240599999999997 24.2 20.8 27.0 14.0 28.0 25-29 23.02221628050385 24.2 20.4 27.0 14.0 28.0 30-34 23.219923501720313 24.2 21.2 27.0 14.6 28.0 35-39 23.33062473031506 24.2 21.0 27.0 14.8 28.0 40-44 22.92314969495828 23.8 20.6 27.0 14.0 28.0 45-49 22.97872666674462 24.4 20.6 27.0 14.0 28.0 50-54 22.80074340415247 23.8 20.4 26.8 14.0 28.0 55-59 22.775292602477354 24.0 20.0 27.0 14.0 28.0 60-64 22.428198162067734 23.6 20.0 27.0 13.8 28.0 65-69 22.260396058215257 23.4 19.8 26.8 14.0 28.0 70-74 21.560624770464305 23.0 19.0 26.0 12.6 28.0 75-79 21.936673679127022 23.0 19.4 26.0 13.0 28.0 80-84 21.753273608650296 22.8 19.0 26.2 13.2 27.8 85-89 21.312291256405295 22.4 18.0 26.0 12.8 28.0 90-94 20.83714954971945 21.8 17.4 25.2 12.6 27.0 95-99 21.00797257321427 22.2 17.8 25.4 12.6 27.0 100-104 21.26095451947078 22.2 18.0 25.4 12.8 27.0 105-109 21.27576399775284 22.2 18.4 25.0 13.6 27.0 110-114 20.934842294763268 22.0 17.6 25.0 12.6 27.0 115-119 20.76678974326024 22.0 17.6 25.0 12.4 27.0 120-124 21.1771936887356 22.4 18.2 25.2 13.0 27.0 125-129 21.446026984643957 22.6 18.8 26.0 13.0 27.2 130-134 21.29566541821846 22.4 18.8 25.6 13.0 27.2 135-139 20.895901150996284 22.0 18.4 25.0 13.0 27.0 140-144 20.67034724781508 21.4 17.8 24.6 13.0 27.0 145-149 20.65367428226565 22.0 17.8 24.6 13.0 26.8 150-154 20.656073034372422 22.0 17.4 24.8 13.0 27.0 155-159 20.540116719734264 21.6 17.0 24.4 12.8 26.8 160-164 20.114044788660237 21.2 16.0 24.2 12.0 26.0 165-169 20.278991645872807 21.6 16.6 24.2 12.2 26.0 170-174 20.302488788748263 21.2 17.4 24.0 12.6 25.8 175-179 19.615445742541027 20.6 15.2 23.6 12.0 25.4 180-184 18.875084681183246 20.0 14.2 23.2 11.2 25.0 185-189 20.034049082064033 20.6 16.8 23.8 12.8 25.8 190-194 19.85577259260682 20.8 16.2 24.0 12.6 25.4 195-199 19.843512479052134 20.6 14.4 23.8 12.0 25.8 200-204 19.842270172195406 20.0 16.0 23.5 12.0 25.5 205-209 19.93344390387734 NaN NaN NaN NaN NaN 210-214 19.522481356386216 NaN NaN NaN NaN NaN 215-219 18.304776403217346 NaN NaN NaN NaN NaN 220-224 19.472121212121213 NaN NaN NaN NaN NaN 225-229 17.57595238095238 NaN NaN NaN NaN NaN 230-234 16.79 NaN NaN NaN NaN NaN 235-239 18.1 NaN NaN NaN NaN NaN 240-244 24.0 NaN NaN NaN NaN NaN 245-249 23.0 NaN NaN NaN NaN NaN 250-254 14.6 NaN NaN NaN NaN NaN 255-259 17.2 NaN NaN NaN NaN NaN 260 19.0 NaN NaN NaN NaN NaN >>END_MODULE >>Per sequence quality scores warn #Quality Count 15 4.0 16 21.0 17 75.0 18 171.0 19 373.0 20 526.0 21 558.0 22 550.0 23 550.0 24 489.0 25 408.0 26 223.0 27 50.0 28 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.65 34.175 27.1 17.075000000000003 2 38.875 36.675000000000004 6.375 18.075 3 21.475 13.425 11.675 53.425 4 37.275000000000006 28.425 18.175 16.125 5 17.375 26.375 27.575 28.675 6 32.225 19.125 8.0 40.65 7 32.074999999999996 22.825 14.124999999999998 30.975 8 17.625 20.849999999999998 26.35 35.175 9 18.45 18.025 15.525 48.0 10-14 28.68 14.545 21.02 35.754999999999995 15-19 38.97 16.49 13.355 31.185000000000002 20-24 31.540000000000003 18.8 20.415 29.244999999999997 25-29 30.983795715647418 18.18090603521798 18.777905985049916 32.05739226408468 30-34 29.358215748748297 17.73630708541951 16.67931017043443 36.22616699539776 35-39 32.58971871968962 22.185920669763643 17.091224666904896 28.13313594364184 40-44 34.0219027352468 20.662272279026315 17.79726994342658 27.518555042300306 45-49 22.837588839168202 19.45775204001053 20.958146880758093 36.746512240063176 50-54 27.769985974754558 19.713884992987378 21.65497896213184 30.861150070126225 55-59 21.36208318604925 23.288558972546248 22.675857193354542 32.67350064804996 60-64 26.098461538461535 20.276923076923076 24.043076923076924 29.581538461538457 65-69 27.252281197789486 14.663924945379772 27.71494666495309 30.368847191877652 70-74 27.288357059349426 18.47190702152534 29.667835774706003 24.571900144419228 75-79 19.63646772577644 19.965650493774152 23.901531415485902 36.496350364963504 80-84 25.894403856292836 24.350380357008362 21.563606236348573 28.19160955035023 85-89 24.531091913767607 22.803330998521286 24.266479881702857 28.39909720600825 90-94 20.237002420095134 24.209296503379786 20.896269715430194 34.657431361094886 95-99 23.23930506258173 19.213525126097515 27.918923967868487 29.628245843452273 100-104 17.457324335053592 19.720127034537516 27.312425565700675 35.51012306470822 105-109 24.972375690607734 20.508287292817677 21.049723756906076 33.469613259668506 110-114 29.827544328394463 18.132135049793536 26.232693709011418 25.807626912800586 115-119 20.888602704443013 14.616870573084354 31.216999356084997 33.27752736638764 120-124 26.18918918918919 14.310810810810812 25.054054054054053 34.445945945945944 125-129 28.535871156661784 14.743777452415813 26.23718887262079 30.48316251830161 130-134 23.39382050024522 19.061631518718325 20.66372404773582 36.88082393330064 135-139 21.840466144263612 21.981113120353626 23.628691983122362 32.5497287522604 140-144 26.14213197969543 21.82741116751269 24.5269958467928 27.503461005999075 145-149 22.132390096008084 22.915613946437592 24.63365336028297 30.318342597271347 150-154 26.22086570477247 22.752497225305216 19.284128745837958 31.74250832408435 155-159 29.208726799088247 25.236079452946925 18.430478671442525 27.124715076522303 160-164 26.1271676300578 24.43159922928709 17.533718689788053 31.90751445086705 165-169 28.22085889570552 24.256724870221802 18.452100047192072 29.0703161868806 170-174 23.569405099150142 25.26912181303116 19.716713881019828 31.444759206798867 175-179 26.6016713091922 13.3008356545961 26.253481894150415 33.844011142061284 180-184 28.063241106719367 14.071146245059287 25.691699604743086 32.17391304347826 185-189 24.830261881668285 21.62948593598448 20.950533462657614 32.58971871968962 190-194 21.831869510664994 26.348808030112924 20.200752823086574 31.618569636135508 195-199 23.114754098360656 18.852459016393443 30.983606557377048 27.049180327868854 200-204 25.651302605210418 16.83366733466934 33.26653306613226 24.248496993987974 205-209 26.345609065155806 21.813031161473088 26.912181303116146 24.929178470254957 210-214 18.773946360153257 28.735632183908045 23.754789272030653 28.735632183908045 215-219 20.73170731707317 35.36585365853659 22.5609756097561 21.341463414634145 220-224 27.27272727272727 29.09090909090909 12.727272727272727 30.909090909090907 225-229 23.52941176470588 20.588235294117645 20.588235294117645 35.294117647058826 230-234 29.166666666666668 29.166666666666668 29.166666666666668 12.5 235-239 13.333333333333334 6.666666666666667 53.333333333333336 26.666666666666668 240-244 0.0 60.0 20.0 20.0 245-249 0.0 100.0 0.0 0.0 250-254 0.0 80.0 0.0 20.0 255-259 0.0 100.0 0.0 0.0 260 0.0 0.0 100.0 0.0 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.5 26 0.5 27 1.0 28 2.5 29 2.0 30 2.5 31 5.0 32 13.166666666666666 33 25.333333333333332 34 48.666666666666664 35 43.5 36 18.5 37 15.0 38 17.5 39 22.833333333333336 40 21.833333333333332 41 30.833333333333332 42 30.166666666666664 43 22.5 44 30.0 45 74.5 46 133.0 47 174.5 48 121.0 49 48.0 50 38.5 51 43.5 52 59.5 53 53.0 54 48.5 55 57.5 56 55.0 57 45.5 58 69.5 59 120.0 60 177.0 61 241.83333333333331 62 324.8333333333333 63 429.8333333333336 64 385.33333333333377 65 291.83333333333326 66 246.33333333333334 67 161.0 68 152.33333333333334 69 143.0 70 107.5 71 76.0 72 49.5 73 52.5 74 55.0 75 140.0 76 235.5 77 136.0 78 32.5 79 17.5 80 5.5 81 7.5 82 7.5 83 4.5 84 1.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-154 0.0 155-159 0.0 160-164 0.0 165-169 0.0 170-174 0.0 175-179 0.0 180-184 0.0 185-189 0.0 190-194 0.0 195-199 0.0 200-204 0.0 205-209 0.0 210-214 0.0 215-219 0.0 220-224 0.0 225-229 0.0 230-234 0.0 235-239 0.0 240-244 0.0 245-249 0.0 250-254 0.0 255-259 0.0 260 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 25-29 34.0 30-34 29.0 35-39 49.0 40-44 68.0 45-49 68.0 50-54 285.0 55-59 161.0 60-64 114.0 65-69 243.0 70-74 96.0 75-79 163.0 80-84 81.0 85-89 98.0 90-94 272.0 95-99 171.0 100-104 158.0 105-109 201.0 110-114 139.0 115-119 48.0 120-124 104.0 125-129 147.0 130-134 170.0 135-139 214.0 140-144 68.0 145-149 63.0 150-154 110.0 155-159 88.0 160-164 94.0 165-169 80.0 170-174 76.0 175-179 43.0 180-184 38.0 185-189 50.0 190-194 40.0 195-199 30.0 200-204 23.0 205-209 25.0 210-214 16.0 215-219 28.0 220-224 7.0 225-229 3.0 230-234 1.0 235-239 3.0 240-244 0.0 245-249 0.0 250-254 0.0 255-259 0.0 260-261 1.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.875 #Duplication Level Percentage of deduplicated Percentage of total 1 81.67247386759581 29.299999999999997 2 7.247386759581882 5.2 3 2.7177700348432055 2.9250000000000003 4 0.975609756097561 1.4000000000000001 5 0.975609756097561 1.7500000000000002 6 0.4181184668989547 0.8999999999999999 7 0.4878048780487805 1.225 8 0.34843205574912894 1.0 9 0.34843205574912894 1.125 >10 4.2508710801393725 29.225 >50 0.2787456445993031 7.55 >100 0.2787456445993031 18.4 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGCGTCCTCTGCTGGGGGCTGTGACGTTGCCCATGAGACTGCGATTCTCG 268 6.7 No Hit GACACGGATTCCACCGCGTCCCGGGCAGCCGGAGGTGGTGGGGGTGGCGG 188 4.7 No Hit AACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGA 159 3.975 No Hit CAGAAGAACTCGCTGGTGCAGTTCCACGTGCTGTCCACGGCGGTGCCCAT 121 3.025 No Hit GTGTTCGCGCCCACGGACAACGCGTTCACGAGCCTGGCTTCCGGCACGCT 97 2.4250000000000003 No Hit TGCTGGGGGCTGTGACGTTGCCCATGAGACTGCGATTCTCGGTCTACACG 74 1.8499999999999999 No Hit CAGAAGAACTCGCTGGTGCAGTTCCACGTGCTGTCCACGGCGGTGCCCATGTCGCAGTTCGACACCG 73 1.825 No Hit AACTCGCTTTCGGACAGCCAGAAGAACTCGCTGGTGCAGTTCCACGTGCT 58 1.4500000000000002 No Hit AACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCG 48 1.2 No Hit ACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCAT 46 1.15 No Hit AACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGT 41 1.0250000000000001 No Hit TGGTCACTTTTAATTTCACAAATGTTTTCTTCCTAGTCAATTTTCTTTGA 39 0.975 No Hit ACTCCAATCTTGTTATAACTAAGCCACAGCTAAGCTCGCAAGCTCTCCAC 35 0.8750000000000001 No Hit CAACGCGTTCACGAGCCTGGCTTCCGGCACGCTCAACTCGCTTTCGGACA 33 0.8250000000000001 No Hit AGCTCAACAGCTCCCAGACGGGCGGGCTCACGGTGTTCGCGCCCACGGAC 32 0.8 No Hit TCTGCTGGGGGCTGTGACGTTGCCCATGAGACTGCGATTCTCGGTCTACA 31 0.775 No Hit CGTCGACAACACGCTCTTCACCGGCGACCAGCTCGTGGTCTACCAGGTCA 31 0.775 No Hit AGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACACGCTCTTCACC 29 0.7250000000000001 No Hit AGCCTGGCTTCCGGCACGCTCAACTCGCTTTCGGACAGCCAGAAGAACTC 28 0.7000000000000001 No Hit TAAGCCACAGCTAAGCTCGCAAGCTCTCCACCAGATAGATCACTCTCGCA 27 0.675 No Hit GACACGGATTCCACCGCGTCCCGGGCAGCCGGAGGTGGTGGGGGTGGCCA 25 0.625 No Hit AGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAGC 24 0.6 No Hit AGCAGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACACG 24 0.6 No Hit CCAGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACCGTTCATCCG 24 0.6 No Hit GGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCA 22 0.5499999999999999 No Hit AACCCGCTCAGGACGCAGGCCGGGAGCAGCTCCCCGGGGGAGTACCCGCTCAACGTCACCGCCACCGG 22 0.5499999999999999 No Hit CAGTTCGACACCGTGAGCAACCCGCTCAGGACGCAGGCCGGGAGCAGCTC 21 0.525 No Hit GGCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAG 21 0.525 No Hit CAGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAG 20 0.5 No Hit CCAGATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTAC 20 0.5 No Hit AGTGCTGCTCGTGATCTCAGCGGCGATCACGGCGTCGGCGCAGGGCCCAACGGCGGCGCCAACGACTCC 19 0.475 No Hit CAGCAGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACAC 19 0.475 No Hit AGGACGCAGGCCGGGAGCAGCTCCCCGGGGGAGTACCCGC 18 0.44999999999999996 No Hit TGCAGTTCCACGTGCTGTCCACGGCGGTGCCCATGTCGCAGTTCGACACC 17 0.42500000000000004 No Hit TGTCCACGGCGGTGCCCATGTCGCAGTTCGACACCGTGAGCAACCCGCTC 17 0.42500000000000004 No Hit ACAGCTCCCAGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCG 17 0.42500000000000004 No Hit ACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGAC 16 0.4 No Hit ACAACACGCTCTTCACCGGCGACCAGCTCGTGGTCTACCAGGTCAACCAG 16 0.4 No Hit AGCAGCTCCCCGGGGGAGTACCCGCTCAACGTCACCGCCACCGGCCAGCA 15 0.375 No Hit CCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACATAA 15 0.375 No Hit GTTCGCGCCCACGGACAACGCGTTCACGAGCCTGGCTTCCGGCACGCTCA 14 0.35000000000000003 No Hit TGCGTCCTCTGCTGGGGGGCTGTGACGTTGCCCATGAGACTGCGATTCTC 14 0.35000000000000003 No Hit GCTCAGGACGCAGGCCGGGTCAACGTCACCGCCACCGGCCAGCAGGTCAACATCTCCACG 14 0.35000000000000003 No Hit CATGTCGCAGTTCGACACCGTGAGCAACCCGCTCAGGACGCAGGCCGGGA 14 0.35000000000000003 No Hit AACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGC 14 0.35000000000000003 No Hit AACGACTCCAGCCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAG 14 0.35000000000000003 No Hit GTGGGGGTGGCGGCGTCGTCGCGGTGGCCATGGCATTGGCGTCCTGCGTC 14 0.35000000000000003 No Hit TAACAGGCGTGCTAGCGAAGCCGGGCAGTTCAACACGTTCATCCGGCTGC 13 0.325 No Hit GACACAAGACGACGACTGCCTGGAGGTGTACAAAGACAAGGATGTGCTGG 13 0.325 No Hit GGGCTGTGACGTTGCCCATGAGACTGCGATTCTCGGTCTACACGTCGAGT 13 0.325 No Hit ACCACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATC 13 0.325 No Hit CCGGGAGCAGCTCCCCGGGGGAGTACCCGCTCAACGTCACCGCCACCGGC 13 0.325 No Hit ACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGA 13 0.325 No Hit TAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGGCTG 13 0.325 No Hit GTGTTCGCGCCCACGGACACGCGTTCACGAGCCTGGCTTCCGGCACGCTC 12 0.3 No Hit AATGGAGCCATGCAGAGATCATTTCTAGTGCTGCTCGTGATCTCAGCGGC 12 0.3 No Hit CATTGGCGTCCTGCGTCCTCTGCTGGGGGCTGTGACGTTGCCCATGAGAC 12 0.3 No Hit TGCGTCCTCTGCTGGGGGCTGGTGACGTTGCCCATGAGACAGATTTAGTA 12 0.3 No Hit AACGTCACCGCCACCGGCCAGCAGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCG 12 0.3 No Hit TGCGTCCTCTGCTGGGGGCTGTGACGTTGCCCATGAGACAGATTTAGTAA 12 0.3 No Hit GTGGTGGGGGTGGCGGCGTCGTCGCGGTGGCCATGGCATTGGCGTCCTGCGTCCTCTGCTGGG 11 0.27499999999999997 No Hit CCACCGGCCAGCAGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACACGCTCTTCACCGG 11 0.27499999999999997 No Hit AGACGGGCGGGCGTCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAG 11 0.27499999999999997 No Hit ACTAATCCCCAATCAATCGATCTTCTTTGGATGAATCTTGTTTGATGTAT 11 0.27499999999999997 No Hit TGTTCGCGCCCACGGACAACGCGTTCACGAGCCTGGCTTCCGGCACGCTC 11 0.27499999999999997 No Hit AGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACACGCGTTCACGAGCC 11 0.27499999999999997 No Hit GCTCAACTCGCTTTCGGACAGCCAGAAGAACTCGCTGGTGCAGTTCCACGTGC 10 0.25 No Hit GCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCACGGGCGTCGCCGCCC 10 0.25 No Hit AATGGAAGCCATGCAGAGATCATTTCTAGTGCTGCTCGTGATCTCAGCGG 10 0.25 No Hit CCCAAATCGACAACCAGCTCAACAGCTCCCAGACGGGCGGGCTCACGGTG 9 0.22499999999999998 No Hit TGCGTCCTCTGCTGGGGGCTGTGACGTTGCCCATGAGAGCTGCGATTCTC 9 0.22499999999999998 No Hit GAGGTGGTGGGGGTGGCGGCGTCGTCGCGGTGGCCATGGCATTGGCGTCC 9 0.22499999999999998 No Hit CTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACA 9 0.22499999999999998 No Hit AAAAAAGGAGCGTGAGAGCCAAATGAATCGAAAGATTCATGTTTGGTTCG 9 0.22499999999999998 No Hit TGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCA 8 0.2 No Hit AACATAACAGGCGTGCTAGCGAAAAGCCGGGCAGTTCAACACGTTCATCC 8 0.2 No Hit CGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTC 8 0.2 No Hit ACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGG 8 0.2 No Hit AACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGCCGACCAACA 8 0.2 No Hit GGTGTTCGCGCCCACGGACAACGCGTTCACGAGCCTGGCTTCCGGCACGC 7 0.17500000000000002 No Hit GGTGTCGCGCCCACGGACAACGCGTTCACGAGCCTGGCTTCCGGCACGCT 7 0.17500000000000002 No Hit GACACGGATTCCACCGCGTCCCGGGCAGCCGGAGGTGGTGGGGTGGCGG 7 0.17500000000000002 No Hit GTGTTCGCGCCCACGGACAACGCGTCACGAGCCTGGCTTCCGGCACGCTC 7 0.17500000000000002 No Hit TGCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGG 7 0.17500000000000002 No Hit CAGAAGAACTCGCTGGTGCAGTTCCACGTGCTGTCCACGGCGGTGCCATG 7 0.17500000000000002 No Hit TGGTCACTTTTAATTTCACAATGTTTTCTTCCTAGTCAATTTTCTTTGAC 7 0.17500000000000002 No Hit AGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACACGCTC 6 0.15 No Hit ACGTTCATCCGGCTGCTGCGTTCCACGGGCGTCGCCGCCCAAATCGACAA 6 0.15 No Hit AACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAGAC 6 0.15 No Hit ACGTTGCCCATGAGACTGCGATTCTCGGTCTACACGTCGAGTTTTTTTTT 6 0.15 No Hit ACGTTCATCCGGCTGCTGCGTTCCACGGCGTCGCCGCCCAAATCGACAAC 6 0.15 No Hit ACGTTCATCCGGCTGCTGCGTTCCACGGGCGTCGCCGCCCCAAATCGACA 6 0.15 No Hit GCCAAGGCGAGGGCTGCTCGTGATCTCAGCGGCGATCACGGCGTCGGCGC 5 0.125 No Hit AACATAACAGGCGTGCTAGCGAAGCCGGGCAGTTCAACACGTTCATCCGG 5 0.125 No Hit TGCGTCCTCTGCTGGGGGCTGTGACGTTGCCATGAGACTGCGATTCTCGG 5 0.125 No Hit CCCAGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCACG 5 0.125 No Hit TGCGTCCTCTGCTGGGGGCTGTGACGTTGCCCATG 5 0.125 No Hit ACTCCATCTTGTTATAACTAAGCCACAGCTAAGCTCGCAAGCTCTCCACC 5 0.125 No Hit CAGAAGAACTCGCTGGTGCAGTTCCACGTGCTGTCCACGGCGGTGCCCAC 5 0.125 No Hit ACTCCAATCTTGTTATAACTAGCCACAGCTAAGCTCGCAAGCTCTCCACC 5 0.125 No Hit ACCGGCAGCCGGAACGACCAAGACGACCACATAACAGGCGTGCTAGCGAA 5 0.125 No Hit CAGCAGGTCAACATCTCCACGGGGTCGTCAATGCCACCGTCGACAACACG 5 0.125 No Hit TGCTGGGGGCTGTGACGTTGCCCATGAGACTGCGAGTTCTCGGTCTACAC 5 0.125 No Hit ACCGCGTCCCGGGCAGCCGGAGGTGGTGGGGGTGGCGG 5 0.125 No Hit TGCGTCCTCTGCTGGGGGCTGTGACGTTGCCCATGAGACTGCGATCTCGG 5 0.125 No Hit CGTCGACACACGCTCTTCACCGGCGACCAGCTCGTGGTCTACCAGGTCAA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-14 0.0 0.0 0.0 0.0 0.0 15-19 0.0 0.0 0.0 0.0 0.0 20-24 0.0 0.0 0.0 0.0 0.0 25-29 0.0 0.0 0.0 0.0 0.0 30-34 0.0 0.0 0.0 0.0 0.0 35-39 0.0 0.0 0.0 0.0 0.0 40-44 0.0 0.0 0.0 0.0 0.0 45-49 0.0 0.0 0.0 0.0 0.0 50-54 0.0 0.0 0.0 0.0 0.0 55-59 0.0 0.0 0.0 0.0 0.0 60-64 0.0 0.0 0.0 0.0 0.0 65-69 0.0 0.0 0.0 0.0 0.0 70-74 0.0 0.0 0.0 0.0 0.0 75-79 0.0 0.0 0.0 0.0 0.0 80-84 0.0 0.0 0.0 0.0 0.0 85-89 0.0 0.0 0.0 0.0 0.0 90-94 0.0 0.0 0.0 0.0 0.0 95-99 0.0 0.0 0.0 0.0 0.0 100-104 0.0 0.0 0.0 0.0 0.0 105-109 0.0 0.0 0.0 0.0 0.0 110-114 0.0 0.0 0.0 0.0 0.0 115-119 0.0 0.0 0.0 0.0 0.0 120-124 0.0 0.0 0.0 0.0 0.0 125-129 0.0 0.0 0.0 0.0 0.0 130-134 0.0 0.0 0.0 0.0 0.0 135-139 0.0 0.0 0.0 0.0 0.0 140-144 0.0 0.0 0.0 0.0 0.0 145-149 0.0 0.0 0.0 0.0 0.0 150-154 0.0 0.0 0.0 0.0 0.0 155-159 0.0 0.0 0.0 0.0 0.0 160-164 0.0 0.0 0.0 0.0 0.0 165-169 0.0 0.0 0.0 0.0 0.0 170-174 0.0 0.0 0.0 0.0 0.0 175-179 0.0 0.0 0.0 0.0 0.0 180-184 0.0 0.0 0.0 0.0 0.0 185-189 0.0 0.0 0.0 0.0 0.0 190-194 0.0 0.0 0.0 0.0 0.0 195-199 0.0 0.0 0.0 0.0 0.0 200-204 0.0 0.0 0.0 0.0 0.0 205-209 0.0 0.0 0.0 0.0 0.0 210-214 0.0 0.0 0.0 0.0 0.0 215-219 0.0 0.0 0.0 0.0 0.0 220-224 0.0 0.0 0.0 0.0 0.0 225-229 0.0 0.0 0.0 0.0 0.0 230-234 0.0 0.0 0.0 0.0 0.0 235-239 0.0 0.0 0.0 0.0 0.0 240-244 0.0 0.0 0.0 0.0 0.0 245-248 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTTCGG 5 7.4835325E-4 2701.3333 185-189 CGCTTTC 70 0.0 578.8572 190-194 TCGGACA 70 0.0 578.8572 195-197 GCTTCGG 10 2.0039924E-4 450.22223 180-184 TAATCTG 15 0.0014638363 234.89854 170-174 GTAATCT 15 0.0014638363 234.89854 170-174 GGGATGT 30 0.002322323 150.07407 165-169 GTGTAAT 15 0.008767214 131.77235 165-169 AGTGTAA 15 0.008767214 131.77235 165-169 AAGTGTA 15 0.008767214 131.77235 165-169 AAAGTGT 15 0.008767214 131.77235 165-169 ACACGGA 25 2.611523E-7 101.3 2 GGATTCC 25 2.611523E-7 101.3 6 CGGATTC 25 2.611523E-7 101.3 5 GACACGG 25 2.611523E-7 101.3 1 TTCCACC 25 2.611523E-7 101.3 9 GATTCCA 25 2.611523E-7 101.3 7 ATTCCAC 25 2.611523E-7 101.3 8 ACGGATT 25 2.611523E-7 101.3 4 CACGGAT 25 2.611523E-7 101.3 3 >>END_MODULE Read 883597 spots for ERR1942992.sra Written 883597 spots for ERR1942992.sra Read 883597 spots for ERR1942992.sra Written 883597 spots for ERR1942992.sra Read 883597 spots for ERR1942992.sra Written 883597 spots for ERR1942992.sra Read 883597 spots for ERR1942992.sra Written 883597 spots for ERR1942992.sra Read 883597 spots for ERR1942992.sra Written 883597 spots for ERR1942992.sra Read 883597 spots for ERR1942992.sra Written 883597 spots for ERR1942992.sra Read 883597 spots for ERR1942992.sra Written 883597 spots for ERR1942992.sra Read 883597 spots for ERR1942992.sra Written 883597 spots for ERR1942992.sra Read 883597 spots for ERR1942992.sra Written 883597 spots for ERR1942992.sra Read 883597 spots for ERR1942992.sra Written 883597 spots for ERR1942992.sra Read 883597 spots for ERR1942992.sra Written 883597 spots for ERR1942992.sra Read 883597 spots for ERR1942992.sra Written 883597 spots for ERR1942992.sra Read 883597 spots for ERR1942992.sra Written 883597 spots for ERR1942992.sra Read 883597 spots for ERR1942992.sra Written 883597 spots for ERR1942992.sra Read 883597 spots for ERR1942992.sra Written 883597 spots for ERR1942992.sra Read 883597 spots for ERR1942992.sra Written 883597 spots for ERR1942992.sra Read 883616 spots for ERR1942992.sra Written 883616 spots for ERR1942992.sra Read 883597 spots for ERR1942992.sra Written 883597 spots for ERR1942992.sra Read 883597 spots for ERR1942992.sra Written 883597 spots for ERR1942992.sra Read 883597 spots for ERR1942992.sra Written 883597 spots for ERR1942992.sra SRR ids: ['ERR1942992.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_svp0kygt ERR1942992.sra spots: 17671959 blocks: [[1, 883597], [883598, 1767194], [1767195, 2650791], [2650792, 3534388], [3534389, 4417985], [4417986, 5301582], [5301583, 6185179], [6185180, 7068776], [7068777, 7952373], [7952374, 8835970], [8835971, 9719567], [9719568, 10603164], [10603165, 11486761], [11486762, 12370358], [12370359, 13253955], [13253956, 14137552], [14137553, 15021149], [15021150, 15904746], [15904747, 16788343], [16788344, 17671959]] ERR1942992 file size 4685270 ERR1942992 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR1942992 ERR1942992_1.fastq Input file: ERR1942992_1.fastq trimmed: ERR1942992-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Tue Dec 10 00:45:16 2024 >> started Tue Dec 10 00:45:25 2024 >> done (9.457s) 17671959 reads processed; of these: 194 ( 0.00%) short reads filtered out after trimming by size control 91 ( 0.00%) empty reads filtered out after trimming by size control 17671674 (100.00%) reads available; of these: 1074209 ( 6.08%) trimmed reads available after processing 16597465 (93.92%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 272 0.00% 19 458 0.00% 20 654 0.00% 21 1073 0.01% 22 1614 0.01% 23 2153 0.01% 24 5553 0.03% 25 30096 0.17% 26 30552 0.17% 27 31799 0.18% 28 31977 0.18% 29 32892 0.19% 30 33725 0.19% 31 33100 0.19% 32 33645 0.19% 33 33642 0.19% 34 34186 0.19% 35 34565 0.20% 36 35641 0.20% 37 36563 0.21% 38 36769 0.21% 39 37723 0.21% 40 38840 0.22% 41 38753 0.22% 42 40831 0.23% 43 41831 0.24% 44 43120 0.24% 45 45295 0.26% 46 46091 0.26% 47 47533 0.27% 48 49092 0.28% 49 49666 0.28% 50 52248 0.30% 51 54657 0.31% 52 54915 0.31% 53 58948 0.33% 54 59260 0.34% 55 61011 0.35% 56 63487 0.36% 57 67472 0.38% 58 68260 0.39% 59 69337 0.39% 60 74167 0.42% 61 73838 0.42% 62 76938 0.44% 63 86497 0.49% 64 82377 0.47% 65 82684 0.47% 66 84914 0.48% 67 87736 0.50% 68 93443 0.53% 69 102883 0.58% 70 99216 0.56% 71 97773 0.55% 72 102815 0.58% 73 100941 0.57% 74 105725 0.60% 75 108081 0.61% 76 108360 0.61% 77 115240 0.65% 78 115803 0.66% 79 114940 0.65% 80 119133 0.67% 81 123722 0.70% 82 125349 0.71% 83 126892 0.72% 84 133032 0.75% 85 134108 0.76% 86 139132 0.79% 87 139539 0.79% 88 140319 0.79% 89 142978 0.81% 90 150525 0.85% 91 148979 0.84% 92 149434 0.85% 93 153361 0.87% 94 153489 0.87% 95 162862 0.92% 96 165098 0.93% 97 171436 0.97% 98 188333 1.07% 99 168655 0.95% 100 165780 0.94% 101 167432 0.95% 102 171441 0.97% 103 170425 0.96% 104 169427 0.96% 105 173302 0.98% 106 171365 0.97% 107 174014 0.98% 108 175427 0.99% 109 179898 1.02% 110 178867 1.01% 111 179223 1.01% 112 184262 1.04% 113 174795 0.99% 114 177120 1.00% 115 177372 1.00% 116 176622 1.00% 117 182296 1.03% 118 180363 1.02% 119 176948 1.00% 120 176217 1.00% 121 171955 0.97% 122 171039 0.97% 123 166215 0.94% 124 165752 0.94% 125 164924 0.93% 126 169569 0.96% 127 163743 0.93% 128 163557 0.93% 129 163091 0.92% 130 163628 0.93% 131 158148 0.89% 132 155027 0.88% 133 149391 0.85% 134 147922 0.84% 135 147254 0.83% 136 145480 0.82% 137 143694 0.81% 138 145247 0.82% 139 140472 0.79% 140 140003 0.79% 141 139005 0.79% 142 131339 0.74% 143 128594 0.73% 144 128197 0.73% 145 126579 0.72% 146 125649 0.71% 147 120698 0.68% 148 117515 0.66% 149 114962 0.65% 150 112719 0.64% 151 107553 0.61% 152 107424 0.61% 153 106264 0.60% 154 105237 0.60% 155 102456 0.58% 156 98156 0.56% 157 94449 0.53% 158 92972 0.53% 159 91863 0.52% 160 89119 0.50% 161 87273 0.49% 162 87214 0.49% 163 86416 0.49% 164 82427 0.47% 165 77794 0.44% 166 74241 0.42% 167 72652 0.41% 168 71357 0.40% 169 68013 0.38% 170 65768 0.37% 171 62928 0.36% 172 60466 0.34% 173 59063 0.33% 174 56988 0.32% 175 54556 0.31% 176 52157 0.30% 177 51030 0.29% 178 49046 0.28% 179 47425 0.27% 180 45845 0.26% 181 43766 0.25% 182 42119 0.24% 183 40353 0.23% 184 39539 0.22% 185 37392 0.21% 186 36194 0.20% 187 34640 0.20% 188 32601 0.18% 189 30889 0.17% 190 28995 0.16% 191 27807 0.16% 192 26805 0.15% 193 25747 0.15% 194 24783 0.14% 195 23737 0.13% 196 22506 0.13% 197 21177 0.12% 198 20202 0.11% 199 19308 0.11% 200 18334 0.10% 201 17324 0.10% 202 16239 0.09% 203 15431 0.09% 204 14730 0.08% 205 13701 0.08% 206 13142 0.07% 207 12459 0.07% 208 11690 0.07% 209 11187 0.06% 210 10744 0.06% 211 10109 0.06% 212 10014 0.06% 213 9667 0.05% 214 9019 0.05% 215 8449 0.05% 216 7798 0.04% 217 7087 0.04% 218 6534 0.04% 219 6078 0.03% 220 5648 0.03% 221 5278 0.03% 222 4882 0.03% 223 4595 0.03% 224 4171 0.02% 225 3821 0.02% 226 3552 0.02% 227 3373 0.02% 228 3090 0.02% 229 2837 0.02% 230 2638 0.01% 231 2422 0.01% 232 2424 0.01% 233 2246 0.01% 234 2008 0.01% 235 1798 0.01% 236 1599 0.01% 237 1466 0.01% 238 1372 0.01% 239 1222 0.01% 240 1128 0.01% 241 1023 0.01% 242 959 0.01% 243 780 0.00% 244 799 0.00% 245 695 0.00% 246 626 0.00% 247 540 0.00% 248 498 0.00% 249 422 0.00% 250 405 0.00% 251 373 0.00% 252 313 0.00% 253 266 0.00% 254 211 0.00% 255 215 0.00% 256 161 0.00% 257 142 0.00% 258 153 0.00% 259 116 0.00% 260 139 0.00% 261 114 0.00% 262 74 0.00% 263 78 0.00% 264 67 0.00% 265 59 0.00% 266 64 0.00% 267 37 0.00% 268 45 0.00% 269 45 0.00% 270 33 0.00% 271 28 0.00% 272 21 0.00% 273 23 0.00% 274 17 0.00% 275 10 0.00% 276 14 0.00% 277 10 0.00% 278 9 0.00% 279 8 0.00% 280 4 0.00% 281 6 0.00% 282 3 0.00% 283 4 0.00% 284 6 0.00% 285 3 0.00% 286 2 0.00% 287 4 0.00% 288 5 0.00% 289 0 0.00% 290 1 0.00% 291 2 0.00% 292 1 0.00% 293 0 0.00% 294 0 0.00% 295 1 0.00% 296 3 0.00% 297 0 0.00% 298 2 0.00% 299 1 0.00% 300 1 0.00% 301 2 0.00% 302 1 0.00% 303 0 0.00% 304 1 0.00% 305 0 0.00% 306 0 0.00% 307 0 0.00% 308 0 0.00% 309 0 0.00% 310 0 0.00% 311 0 0.00% 312 0 0.00% 313 0 0.00% 314 0 0.00% 315 0 0.00% 316 0 0.00% 317 0 0.00% 318 0 0.00% 319 0 0.00% 320 0 0.00% 321 0 0.00% 322 0 0.00% 323 0 0.00% 324 0 0.00% 325 0 0.00% 326 0 0.00% 327 0 0.00% 328 0 0.00% 329 0 0.00% 330 0 0.00% 331 0 0.00% 332 0 0.00% 333 0 0.00% 334 0 0.00% 335 0 0.00% 336 0 0.00% 337 0 0.00% 338 0 0.00% 339 0 0.00% 340 0 0.00% 341 0 0.00% 342 0 0.00% 343 0 0.00% 344 1 0.00% 17671674 reads passed initial QC criterion=sequence-density sequence-density=0.91 sequence-density-rank=1 fanout-score=0.00 fanout-score-rank=42 prefix-density=0.00 prefix-fanout=1.0 sequence=ACAGCTATCACACACAGGCAAAACACAGCTGATTCGTGTACTCGATCTCCCCAGCAAGTTAAGGCCACCATGTCGAGCGGCTGCGGCAACTGCGACTGCGCTGACAAGACCCAGTGTGTGAAGAAGGGAAACGGCTACGGCATCGTCATGGTTGACACCGAGAAGAGCCACTTCGAGGTGCAGGAGTCCGCGGCGGAGAACGACGGCAAGTGCAAGTGCGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=61.36 fanout-score-rank=1 prefix-density=0.11 prefix-fanout=3.4 sequence=TCGGCGCCGCCGGAGGAGTCCTGCTCTGAGAATAGAAAGGAAATCGCTTCGAATTCGGTGAGAATTGAAGACGGTGGCGTTCTCTGCGTGATTGAGCTGGTGTGGTGTATACGTAGTAGTATACGATGCTCCGCATTTGATTGATTTGATCGATTCGGTCGATTTGGTCGGTCGGTCGATTCTTGTACCAAGAACACTTTGTTGCTGTGTATGTGCATCGGCTTTATTCTTTCTTCTTTTTCTT Started job on | Dec 10 00:45:41 Started mapping on | Dec 10 00:45:41 Finished on | Dec 10 00:46:21 Mapping speed, Million of reads per hour | 1590.45 Number of input reads | 17671674 Average input read length | 113 UNIQUE READS: Uniquely mapped reads number | 14775393 Uniquely mapped reads % | 83.61% Average mapped length | 111.56 Number of splices: Total | 4817818 Number of splices: Annotated (sjdb) | 4434540 Number of splices: GT/AG | 4617415 Number of splices: GC/AG | 61335 Number of splices: AT/AC | 2945 Number of splices: Non-canonical | 136123 Mismatch rate per base, % | 0.64% Deletion rate per base | 0.39% Deletion average length | 1.15 Insertion rate per base | 0.41% Insertion average length | 1.22 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 697449 % of reads mapped to multiple loci | 3.95% Number of reads mapped to too many loci | 209707 % of reads mapped to too many loci | 1.19% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 10.14% % of reads unmapped: other | 1.12% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2198832 2198832 2198832 N_multimapping 697449 697449 697449 N_noFeature 494307 600816 14370735 N_ambiguous 343967 53468 1370 UnstrandedReadsAssigned:13937119 PositiveStrandReadsAssigned:14121109 NegativeStrandReadsAssigned:403288 Dataset is classified positive stranded MeadianReadLen=116 20thPercentileLength=82 echo kmer=77 ERR1942992 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR1942992-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 17,671,674 reads, 14,969,358 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,172 rounds 52973 ERR1942992.ke.tsv 35125 ERR1942992.se.tsv 88098 total ==> ERR1942992.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 151.114 17.334 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 94.886 6.64 PNS24243 293 194 0 0 KQK14069 1603 1504 5236.91 334.309 KQK14071 474 375 70.594 18.0741 ==> ERR1942992.se.tsv <== BRADI_1g14170v3 5128 BRADI_1g53295v3 82 BRADI_1g59795v3 179 BRADI_1g07683v3 0 BRADI_1g00485v3 22 BRADI_1g20270v3 539 BRADI_1g74790v3 106 BRADI_1g09890v3 51 BRADI_1g77505v3 297 BRADI_1g48960v3 0 ERR1942992 completed mapping pipeline successfully