Starting /dee2/code/volunteer_pipeline.sh ERR1942993 current disk space = 1523534704640 free memory = 1448287496 ERR1942993 SRAfilesize 40854dc007201cbbb54761c70ca413bd ERR1942993.sra ERR1942993.sra file validated ERR1942993 is single end ERR1942993 is conventional basespace ERR1942993 read1 length is 25-246 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1942993_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 25-246 %GC 51 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.92475 26.0 23.0 28.0 20.0 29.0 2 24.16575 26.0 22.0 28.0 17.0 29.0 3 24.52775 26.0 23.0 28.0 19.0 29.0 4 24.7715 26.0 23.0 28.0 19.0 29.0 5 24.71075 26.0 23.0 28.0 19.0 29.0 6 24.4615 26.0 23.0 28.0 19.0 29.0 7 24.23125 25.0 22.0 27.0 19.0 29.0 8 24.8165 26.0 23.0 28.0 20.0 29.0 9 24.67275 26.0 23.0 28.0 19.0 29.0 10-14 24.63005 26.0 22.8 28.0 19.0 29.0 15-19 24.852250000000005 26.4 22.8 28.0 19.8 29.0 20-24 24.47305 25.8 22.8 27.8 18.6 28.8 25-29 24.51396370007427 26.2 22.8 28.0 18.2 29.0 30-34 24.667170840801774 25.8 22.6 28.0 19.4 29.0 35-39 24.741540373669608 26.0 22.8 27.8 19.6 29.0 40-44 24.394319617772332 26.0 22.6 28.0 17.8 29.0 45-49 24.387151665142976 26.0 22.8 28.0 18.4 28.8 50-54 24.397865839292535 26.0 22.6 27.8 18.6 29.0 55-59 24.336805995663894 25.8 22.4 27.8 18.6 28.4 60-64 24.19285286432656 25.6 22.2 27.8 17.0 28.8 65-69 23.886671443008076 25.4 22.0 27.2 14.6 28.0 70-74 23.597817466961242 25.0 21.4 27.0 14.2 28.0 75-79 23.414249147543323 24.8 21.0 27.0 14.2 28.0 80-84 23.667449726887078 25.0 21.8 27.0 15.0 28.0 85-89 23.29349264185964 24.4 20.8 27.0 14.0 28.0 90-94 23.23027839045448 24.4 20.8 27.0 14.2 28.0 95-99 22.730721738646675 24.2 20.0 27.0 14.0 28.0 100-104 22.553319920170456 23.8 20.0 27.0 13.6 28.0 105-109 22.06456605258695 23.2 19.2 26.8 13.4 28.0 110-114 22.049012903550988 23.0 19.6 26.2 13.0 28.0 115-119 21.596028787835376 23.0 18.6 26.0 12.8 28.0 120-124 21.840111144009718 22.8 19.2 26.0 13.0 28.0 125-129 21.87227844845888 23.0 19.4 26.0 13.0 27.6 130-134 21.35672428461026 22.6 18.8 26.0 12.8 27.4 135-139 20.734874716677155 21.8 16.8 25.0 12.2 27.0 140-144 20.364700753070466 21.6 15.4 24.8 12.6 27.0 145-149 20.361862263841743 21.2 16.2 24.8 12.6 27.0 150-154 20.410376253282212 21.4 16.6 24.4 12.4 27.0 155-159 19.72530768629253 20.4 14.4 24.2 11.2 26.2 160-164 19.548029177549406 20.0 14.4 23.4 11.6 26.0 165-169 19.813518900162904 20.0 14.8 24.0 12.2 26.2 170-174 20.403182612021983 21.6 16.8 24.4 12.2 26.2 175-179 20.22747068903319 21.666666666666668 18.333333333333332 24.0 13.0 26.0 180-184 19.328915116415114 NaN NaN NaN NaN NaN 185-189 19.389611031779612 NaN NaN NaN NaN NaN 190-194 19.119094806381334 NaN NaN NaN NaN NaN 195-199 19.746292647634 NaN NaN NaN NaN NaN 200-204 19.5030881691408 NaN NaN NaN NaN NaN 205-209 20.19047619047619 NaN NaN NaN NaN NaN 210-214 20.874769230769232 NaN NaN NaN NaN NaN 215-219 18.681484409310496 NaN NaN NaN NaN NaN 220-224 16.958344155844156 NaN NaN NaN NaN NaN 225-229 18.71142857142857 NaN NaN NaN NaN NaN 230-234 20.6 NaN NaN NaN NaN NaN 235-239 19.5 NaN NaN NaN NaN NaN 240-244 16.4 NaN NaN NaN NaN NaN 245-246 20.5 NaN NaN NaN NaN NaN >>END_MODULE >>Per sequence quality scores warn #Quality Count 15 1.0 16 17.0 17 36.0 18 86.0 19 158.0 20 247.0 21 302.0 22 369.0 23 493.0 24 586.0 25 686.0 26 722.0 27 285.0 28 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.15 31.2 27.575 16.075 2 32.775 33.225 12.1 21.9 3 26.8 23.775 19.7 29.725 4 24.25 26.650000000000002 22.725 26.375 5 19.475 28.275 22.875 29.375 6 27.575 21.675 16.275000000000002 34.475 7 27.6 26.275 24.425 21.7 8 18.175 20.625 28.575 32.625 9 15.725 23.0 26.0 35.275 10-14 21.759999999999998 21.634999999999998 26.935 29.67 15-19 27.825 20.79 22.2 29.185 20-24 22.39 23.84 23.02 30.75 25-29 25.239216472120635 19.26256199589199 26.33134612494364 29.166875407043737 30-34 26.139694733650053 20.41847771151319 23.865359344991408 29.57646820984535 35-39 25.749297932090887 23.92647434260914 21.812611692621907 28.511616032678074 40-44 26.743458772021267 18.76889398519754 29.646617325132908 24.841029917648285 45-49 24.702540465215993 18.774788294565333 26.717761817986922 29.804909422231752 50-54 27.570712598651998 20.44472607869117 24.073967394435165 27.910593928221672 55-59 23.399118836381195 18.250950570342205 27.249683143219265 31.100247450057335 60-64 24.43853988739166 21.332321123552855 26.096033402922757 28.13310558613273 65-69 24.704770902220123 21.013563668263714 27.99784061002767 26.283824819488494 70-74 22.66329844821364 22.042583904727536 29.765427643450014 25.528690003608805 75-79 22.337011818110668 21.374344525318932 28.676528136495268 27.612115520075136 80-84 24.446135633350245 26.027397260273972 24.835109081684422 24.691358024691358 85-89 23.332124728063814 23.04205946337926 29.396301667875274 24.229514140681655 90-94 23.630922693266836 28.179551122194514 24.658354114713216 23.531172069825438 95-99 24.247473064534045 28.157280906364544 25.96912140397645 21.62612462512496 100-104 25.129533678756477 30.014803849000742 22.341475450283742 22.514187021959042 105-109 23.043288150415393 29.296020988194137 26.278968080454746 21.381722780935725 110-114 22.841316389703486 23.721081785597914 30.498533724340177 22.939068100358423 115-119 18.485310950019077 27.05074399084319 26.306753147653566 28.157191911484166 120-124 21.96765498652291 29.065588499550763 26.302785265049415 22.66397124887691 125-129 20.27625749283294 32.6296585874381 25.80140734949179 21.292676570237166 130-134 21.643663739021328 29.203262233375156 23.431618569636136 25.72145545796738 135-139 17.956656346749224 30.727554179566564 29.450464396284833 21.86532507739938 140-144 25.368324125230203 30.20257826887661 25.04604051565378 19.38305709023941 145-149 19.643820831084728 37.72261198057205 21.04695089044792 21.586616297895304 150-154 21.226740179186766 34.59682977257064 18.332184700206756 25.844245348035837 155-159 22.49786142001711 31.565440547476477 24.893071000855432 21.04362703165098 160-164 25.803212851405622 30.522088353413658 14.859437751004014 28.815261044176705 165-169 24.200913242009133 27.054794520547947 21.80365296803653 26.94063926940639 170-174 20.685111989459813 30.434782608695656 22.793148880105402 26.08695652173913 175-179 23.063063063063062 16.396396396396394 26.486486486486488 34.054054054054056 180-184 26.980198019801982 23.51485148514851 22.277227722772277 27.22772277227723 185-189 23.303834808259587 23.893805309734514 20.943952802359885 31.858407079646017 190-194 18.928571428571427 23.57142857142857 25.71428571428571 31.785714285714285 195-199 22.321428571428573 26.339285714285715 21.875 29.464285714285715 200-204 20.207253886010363 25.906735751295333 24.352331606217618 29.533678756476682 205-209 21.568627450980394 28.104575163398692 21.568627450980394 28.75816993464052 210-214 25.581395348837212 20.930232558139537 23.25581395348837 30.23255813953488 215-219 24.271844660194176 32.038834951456316 17.475728155339805 26.21359223300971 220-224 23.4375 31.25 14.0625 31.25 225-229 35.0 10.0 15.0 40.0 230-234 20.0 20.0 30.0 30.0 235-239 30.0 30.0 20.0 20.0 240-244 0.0 0.0 66.66666666666666 33.33333333333333 245-246 100.0 0.0 0.0 0.0 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 2.0 25 4.5 26 6.0 27 9.0 28 10.0 29 9.0 30 19.5 31 52.0 32 128.0 33 183.33333333333331 34 300.8333333333333 35 307.0 36 141.0 37 63.0 38 35.66666666666667 39 26.16666666666667 40 18.666666666666668 41 18.0 42 19.5 43 25.5 44 37.5 45 53.0 46 64.0 47 62.5 48 59.5 49 40.0 50 15.5 51 16.5 52 28.0 53 33.0 54 37.0 55 48.0 56 52.0 57 52.5 58 71.5 59 99.0 60 143.5 61 276.0 62 374.33333333333337 63 344.16666666666663 64 258.16666666666663 65 224.66666666666669 66 255.16666666666669 67 220.83333333333334 68 156.0 69 104.5 70 84.5 71 78.5 72 52.5 73 54.0 74 76.0 75 130.5 76 173.0 77 114.5 78 51.0 79 32.5 80 15.5 81 8.5 82 7.0 83 7.5 84 5.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-154 0.0 155-159 0.0 160-164 0.0 165-169 0.0 170-174 0.0 175-179 0.0 180-184 0.0 185-189 0.0 190-194 0.0 195-199 0.0 200-204 0.0 205-209 0.0 210-214 0.0 215-219 0.0 220-224 0.0 225-229 0.0 230-234 0.0 235-239 0.0 240-244 0.0 245-246 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 25-29 28.0 30-34 44.0 35-39 63.0 40-44 82.0 45-49 171.0 50-54 254.0 55-59 131.0 60-64 192.0 65-69 193.0 70-74 185.0 75-79 226.0 80-84 169.0 85-89 164.0 90-94 222.0 95-99 176.0 100-104 242.0 105-109 169.0 110-114 179.0 115-119 181.0 120-124 101.0 125-129 132.0 130-134 132.0 135-139 107.0 140-144 56.0 145-149 79.0 150-154 71.0 155-159 38.0 160-164 29.0 165-169 21.0 170-174 35.0 175-179 40.0 180-184 17.0 185-189 9.0 190-194 15.0 195-199 7.0 200-204 5.0 205-209 9.0 210-214 3.0 215-219 7.0 220-224 9.0 225-229 5.0 230-234 0.0 235-239 0.0 240-244 1.0 245-247 1.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.075 #Duplication Level Percentage of deduplicated Percentage of total 1 72.63389581804842 24.75 2 7.4834922964049895 5.1 3 3.7417461482024947 3.8249999999999997 4 3.2281731474688184 4.3999999999999995 5 1.9075568598679384 3.25 6 2.054292002934703 4.2 7 1.0271460014673515 2.45 8 1.247248716067498 3.4000000000000004 9 1.1005135730007336 3.375 >10 5.209097578870139 32.85 >50 0.293470286133529 8.75 >100 0.07336757153338225 3.65 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTCACTTTTAATTTCACAAATGTTTTCTTCCTAGTCAATTTTCTTTGAC 146 3.65 No Hit GACACGGATTCCACCGCGTCCCGGGCAGCCGGAGGTGGTGGGGGTGGCGG 100 2.5 No Hit TAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGGCTG 88 2.1999999999999997 No Hit GTATCGTTAATACCGTTGTACAATTGCTAGGGATGTAAAAGTGTAATCTG 86 2.15 No Hit TGGTCACTTTTAATTTCACAAATGTTTTCTTCCTAGTCAATTTTCTTTGA 76 1.9 No Hit TATCGTTAATACCGTTGTACAATTGCTAGGGATGTAAAAGTGTAATCTGT 50 1.25 No Hit GGCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAG 49 1.225 No Hit AACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGA 48 1.2 No Hit TTGGTCACTTTTAATTTCACAAATGTTTTCTTCCTAGTCAATTTTCTTTG 48 1.2 No Hit AACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCG 41 1.0250000000000001 No Hit AAAAAAGGAGCGTGAGAGCCAAATGAATCGAAAGATTCATGTTTGGTTCG 40 1.0 No Hit ACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGA 33 0.8250000000000001 No Hit TGCGTCCTCTGCTGGGGGCTGTGACGTTGCCCATGAGACTGCGATTCTCG 33 0.8250000000000001 No Hit ACAACCAGCTCAACAGCTCCCAGACGGGCGGGCTCACGGTGTTCGCGCCC 27 0.675 No Hit AATGTTTTCTTCCTAGTCAATTTTCTTTGACTTGTTCTCTTCCGTATCGT 26 0.65 No Hit AGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAGC 26 0.65 No Hit TAGTCAATTTTCTTTGACTTGTTCTCTTCCGTATCGTTAATACCGTTGTA 26 0.65 No Hit CGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGC 25 0.625 No Hit GTGTTCGCGCCCACGGACAACGCGTTCACGAGCCTGGCTTCCGGCACGCT 24 0.6 No Hit TGACTTGTTCTCTTCCGTATCGTTAATACCGTTGTACAATTGCTAGGGAT 23 0.575 No Hit CAAATGTTTTCTTCCTAGTCAATTTTCTTTGACTTGTTCTCTTCCGTATC 21 0.525 No Hit ACGGACAACGCGTTCACGAGCCTGGCTTCCGGCACGCTCAACTCGCTTTC 21 0.525 No Hit CAGAAGAACTCGCTGGTGCAGTTCCACGTGCTGTCCACGGCGGTGCCCATGTCGCAGTTCGACACCG 21 0.525 No Hit ACTTTTAATTTCACAAATGTTTTCTTCCTAGTCAATTTTCTTTGACTTGT 20 0.5 No Hit AATTTTCTTTGACTTGTTCTCTTCCGTATCGTTAATACCGTTGTACAATT 20 0.5 No Hit ATGTTTTCTTCCTAGTCAATTTTCTTTGACTTGTTCTCTTCCGTATCGTT 19 0.475 No Hit CAGCTCCCCGGGGGAGTACCCGCTCAACGTCACCGCCACCGGCCAGCAGG 18 0.44999999999999996 No Hit ACAGCTCCCAGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCG 18 0.44999999999999996 No Hit TCCTAGTCAATTTTCTTTGACTTGTTCTCTTCCGTATCGTTAATACCGTT 18 0.44999999999999996 No Hit AGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACACGCTC 17 0.42500000000000004 No Hit GGGGTCGTCAATGCCACCGTCGACAACACGCTCTTCACC 17 0.42500000000000004 No Hit GGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCA 17 0.42500000000000004 No Hit TCCCAGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCAC 17 0.42500000000000004 No Hit CTCTTCCGTATCGTTAATACCGTTGTACAATTGCTAGGGATGTAAAAGTG 17 0.42500000000000004 No Hit AGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACACGCTCTTCACC 17 0.42500000000000004 No Hit ACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTACACCACC 17 0.42500000000000004 No Hit ACAACCAGCTCAACAGCTCCCAGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAGC 16 0.4 No Hit GGGGTCGTCAATGCCACCGTCGACAACACGCTCTTCACCGGCGACCAGCT 16 0.4 No Hit GCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCA 15 0.375 No Hit TGCTGGGGGCTGTGACGTTGCCCATGAGACTGCGATTCTCGGTCTACACG 15 0.375 No Hit AGATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTACACCACCAG 15 0.375 No Hit TCACAAATGTTTTCTTCCTAGTCAATTTTCTTTGACTTGTTCTCTTCCGT 15 0.375 No Hit ACGCTCAACTCGCTTTCGGACAGCCAGAAGAACTCGCTGGTGCAGTTCCA 14 0.35000000000000003 No Hit TCGACAACCAGCTCAACAGCTCCCAGACGGGCGGGCTCACGGTGTTCGCG 14 0.35000000000000003 No Hit AAATGTTTTCTTCCTAGTCAATTTTCTTTGACTTGTTCTCTTCCGTATCG 14 0.35000000000000003 No Hit AGATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGAT 14 0.35000000000000003 No Hit CAGCAGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTC 13 0.325 No Hit GCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGG 13 0.325 No Hit ATTTCACAAATGTTTTCTTCCTAGTCAATTTTCTTTGACTTGTTCTCTTC 13 0.325 No Hit GAAGAAGGCGGACGCGCCGGCCCCCGCGCCGCTGGGTCCGGCCAAGAAG 13 0.325 No Hit AGTGCTGCTCGTGATCTCAGCGGCGATCACGGCGTCGGCGCAGGGCCCAACGGCGGCGCCAACGACTCC 13 0.325 No Hit AAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACAC 13 0.325 No Hit CACGCTCAACTCGCTTTCGGACAGCCAGAAGAACTCGCTGGTGCAGTTCC 13 0.325 No Hit CAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACATAACA 13 0.325 No Hit AGTACCCGCTCAACGTCACCGCCACCGGCCAGCAGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACA 13 0.325 No Hit GACACGGATTCCACCGCGTCCCGGGCAGCCGGAGGTGGTGGGGGTGGCG 12 0.3 No Hit ACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCAT 12 0.3 No Hit TTTGGTCACTTTTAATTTCACAAATGTTTTCTTCCTAGTCAATTTTCTTT 12 0.3 No Hit AACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACA 12 0.3 No Hit TCCGGCACGCTCAACTCGCTTTCGGACAGCCAGAAGAACTCGCTGGTGCA 12 0.3 No Hit CGGGGGTCGTCAATGCCACCGTCGACACTCTTCACCGGCGACCAGCTCGTGGAGGGGCCTCT 12 0.3 No Hit CTTGTTCTCTTCCGTATCGTTAATACCGTTGTACAATTGCTAGGGATGTA 12 0.3 No Hit AAGCTCTCCACCAGATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTACACCACCAG 12 0.3 No Hit CACGCTCAACTCGCTTTCGTGGTCGTGCGCAAGCCGGCGCACCCCACGGC 12 0.3 No Hit TCACGAGCCTGGCTTCCGGCACGCTCAACTCGCTTTCGGACAGCCAGAAG 12 0.3 No Hit TTCCACGTGCTGTCCACGGCGGTGCCCATGTCGACAACCAGCTCAACAGC 11 0.27499999999999997 No Hit CCGTCGACAACACGCTCTTCACCGGCCGTCAATGCCATGATGCTGGTGTA 11 0.27499999999999997 No Hit TCGACAACACGCTCTTCACCGGCGACCAGCTCGTGGTCTACCAGGTCAAC 11 0.27499999999999997 No Hit GCCCAAATCGACAACCAGCTCAACAGCTCCCAGACGGG 11 0.27499999999999997 No Hit ACTTGTTCTCTTCCGTATCGTTAATACCGTTGTACAATTGCTAGGGATGT 11 0.27499999999999997 No Hit CAGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAG 10 0.25 No Hit TGCAGAGATCATTTCTAGTGCTGCTCGTGATCTCAGCGGCGATCACGGCG 10 0.25 No Hit CGTTAATACCGTTGTACAATTGCTAGGGATGTAAAAGTGTAATCTGTACC 10 0.25 No Hit CAGAAGAACTCGCTGGTGCAGTTCCACGTGCTGTCCACGGCGGTGCCCATGTCGCAGTTCGACACCGT 10 0.25 No Hit ATTTTCTTTGACTTGTTCTCTTCCGTATCGTTAATACCGTTGTACAATTG 10 0.25 No Hit AATTTCACAAATGTTTTCTTCCTAGTCAATTTTCTTTGACTTGTTCTCTT 10 0.25 No Hit TCACTTTTAATTTCACAAATGTTTTCTTCCTAGTCAATTTTCTTTGACTT 9 0.22499999999999998 No Hit TCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACACGCTCTTCACCGGCGACCAGCTCG 9 0.22499999999999998 No Hit TCGTCAATGCCACCGTCGACAACACGCTCTTCACCGGCGACCAGCTC 9 0.22499999999999998 No Hit TGTTTTCTTCCTAGTCAATTTTCTTTGACTTGTTCTCTTCCGTATCGTTA 9 0.22499999999999998 No Hit TAAGCTCGCAAGCTCTCCACCAGATAGATCACTCTCGCATTTGTCCTCGC 9 0.22499999999999998 No Hit TCCACGGCGGTGCCCATGTCGCAGTTCGACACCGTGAGCAACCCGCTCAG 9 0.22499999999999998 No Hit CGGGCTCACGGTGTTCGCGCCCACGGACAACGCGACGCTCAACTCGCTTT 9 0.22499999999999998 No Hit AGAGATCATTTCTAGTGCTGCTCGTGATCTCAGCGGCGATCACGGCGTCGGCGCAGGGC 9 0.22499999999999998 No Hit AACCAGCTCAACAGCTCCCAGACGGGCGGGCTCACGGTGTTCGCGCCCAC 9 0.22499999999999998 No Hit TGCTTAAAAAATGAAGTTAGCTATTTTTGCAGTGTTATTAATCCGCACCT 9 0.22499999999999998 No Hit TCACGGTGTTCGCGCCCACGGACAACGCGTTCATGAGCCTGGCTTCCGGCACGCTCAACTCGCTTTCGGACAGC 9 0.22499999999999998 No Hit TTCCGGCACGCTCAACTCGCTTTCGGACAGC 9 0.22499999999999998 No Hit TACGTGCTCTGCTTGCATCTCACTCCAATCTTGTTATAACTAAGCCACAG 9 0.22499999999999998 No Hit GGGTGGCGGCGTCGTCGCGGTGGCCATGGCATTGGCGTCCTGCGTCCTCT 9 0.22499999999999998 No Hit ACTTAAAATAAGCGACGGGGTATTGTAAGTGGCAGAGTGGCCTTGCTGCC 9 0.22499999999999998 No Hit CAGCTCCCCGGGGGAGTACCCGCTCAACGTCACCGCCACCGGCCAGC 8 0.2 No Hit GGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAGCCTGGC 8 0.2 No Hit CAGTTCGACACCGTGAGCAACCCGCTCAGGACGCAGGCCGGGAGCAGCTC 8 0.2 No Hit TAGTCAATTTTCTTTGACTTGTCCTCTTCCGTATCGTTAATACCGTTGTA 8 0.2 No Hit ATACTAATAAGATATTGATCCTTCACAAAGGAACCAGTCCCTCTATGCCACATGATATTTCTTCAAATTACGTG 8 0.2 No Hit CGTCGTCGCGGTGGCCATGGCATTGGCGTCCTGCGTCCTCTGCTGGGGGC 8 0.2 No Hit ACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCACGG 8 0.2 No Hit CGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTC 8 0.2 No Hit GCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGC 8 0.2 No Hit GACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGT 8 0.2 No Hit CAGCAGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACAC 8 0.2 No Hit TAATTTCACAAATGTTTTCTTCCTAGTCAATTTTCTTTGACTTGTTCTCT 8 0.2 No Hit CACGAGCCTGGCTTCCGGCACGCTCAACTCGCTTTCGGACAGCCAGAAGA 8 0.2 No Hit GTCACTTTTAATTTCACAAATGTTTTCTTCCTAGTCAATTTTCTTTGACT 8 0.2 No Hit TCAACAGCTCCCAGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAAC 8 0.2 No Hit ACGACTTAAAATAAGCGACGGGGTATTGTAAGTGGCAGAGTGGCCTTGCT 8 0.2 No Hit ACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGCCCACGGAC 8 0.2 No Hit CGGACAACGCGTTCACGAGCCTGGCTTCCGGCACGCTCAACTCGCTTTCGGACAGCCAGAAGAACTCGC 7 0.17500000000000002 No Hit AAGCTCTCCACCAGATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTACACCA 7 0.17500000000000002 No Hit TGCAGAGATCATTTCTAGTGCTGCTCGTGATCTCAGCGGCGATCACGCGTCGGCGCAGGGCCCAACGGCGGCGCC 7 0.17500000000000002 No Hit CGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCG 7 0.17500000000000002 No Hit CAGTTCGACACCGTGAGCAACCCGCTCAGGACGCAGGCCGGGAGCAGCTCCCCGGGGGAGTACCCGC 7 0.17500000000000002 No Hit AATGGAAGCCATGCAGAGATCATTTCTAGTGCTGCTCGTGATCTCAGCGG 7 0.17500000000000002 No Hit CGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGC 7 0.17500000000000002 No Hit ACGGATTCCACCGCGTCCCGGGCAGCCGGAGGTGGTGGGGGTGGCGG 7 0.17500000000000002 No Hit AGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACG 7 0.17500000000000002 No Hit GCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCACGGGCGTCGC 7 0.17500000000000002 No Hit GGGTCGTCAATGCCACCGTCGACAACACGCTCTTCACCGGCGACCAGCTC 7 0.17500000000000002 No Hit AACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAG 7 0.17500000000000002 No Hit GCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGC 7 0.17500000000000002 No Hit AATCTTGTTATAACTAAGCCACAGCTAAGCTCGCAAGCTCTCCACCAGAT 7 0.17500000000000002 No Hit GGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCACGGGCGTCGCCGCC 6 0.15 No Hit ACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAGCCTGGCTTCCGGCACGCTCAACTC 6 0.15 No Hit GCAACCCGCTCAGGACGCAGGCCGGGAGCAGCTCCCCGGGGGAGTA 6 0.15 No Hit CAGCTCCCCGGGGGAGTACCCGCTCAACGTCACCGCCACCGGCCAGCAGGTCAACATCTCCACG 6 0.15 No Hit GACACGGATTCCACCGCGTCCCGGGCAGCCGGAGGTGGTGGGGGTGGGCGG 6 0.15 No Hit TAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCACG 6 0.15 No Hit GCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCACGGGCGTCGCCGCCC 6 0.15 No Hit TTCCACGGGCGTCGCCGCCCAAATCGACAACCAGCTCAACAGCTCCCAGA 6 0.15 No Hit CGGCACGCTCAACTCGCTTTCGGACAGCCAGAAGAACTCGCTGGTGCAGT 6 0.15 No Hit CCACAGTTGCTCAGATAATCTCTGGTTTGAACTATAGTCCAATTTGTCCT 6 0.15 No Hit AACAGCTCCCAGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGC 6 0.15 No Hit TGAGCAACCCGCTCAGGACGCAGGCCGGGAGCAGCTCCCCGGGGGAGTAC 6 0.15 No Hit TTGTTCTCTTCCGTATCGTTAATACCGTTGTACAATTGCTAGGGATGTAA 6 0.15 No Hit GATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTT 6 0.15 No Hit AGTCAATTTTCTTTGACTTGTTCTCTTCCGTATCGTTAATACCGTTGTAC 6 0.15 No Hit AGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAGCCTGGCTTCCGGC 6 0.15 No Hit GAAAAAAGGAGCGTGAGAGCCAAATGAATCGAAAGATTCATGTTTGGTTCGGGA 6 0.15 No Hit CAGAAGAACTCGCTGGTGCAGTTCCACGTGCTGTCCACGGCGGTGCCCAT 6 0.15 No Hit CGGTGGCCATGGCATTGGCGTCCTGCGTCCTCTGCTGGGGGCTGTGACGTTGCCCATGAGACTGC 6 0.15 No Hit TCGCTGCTGACGCGCCTGGCGGCGCTGATGCCGACACGGATTCCACCGCG 6 0.15 No Hit AGGTCAACCAGGTGCTGCTGCCCATGGCCACGGCGACCAGCTCGTGGTCTACC 6 0.15 No Hit AGGGCCCAACGGCGGCGCCAACGACTCCAGCCCCAGCGGCTCCGGC 6 0.15 No Hit ACGTTCATCCGGCTGCTGCGTTCCACGGGCGTCGCCGCCCCAAATCGACA 6 0.15 No Hit ACAAATGTTTTCTTCCTAGTCAATTTTCTTTGACTTGTTCTCTTCCGTAT 6 0.15 No Hit AATGGAAGCCATGCAGAGATCATTTCTAGTGCTGCTCGTGATCTCAGCGGCGATCACGGCGTCGG 6 0.15 No Hit CAGAAGAACTCGCTGGTGCAGTTCCACGTGCTGTCCACGGCGGTGCCCATGTCGCAGTTCGACACC 6 0.15 No Hit TCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACACGCTCTTC 6 0.15 No Hit AATGCCACCGTCGACAACACGCTCTTCACCGGCGACCAGCTCGTGGTCTA 6 0.15 No Hit TCCACGGGGGTCGTCAATGCCACCGTCGACAACACGCTCTTCACGGCCAGCAGGTCAACA 5 0.125 No Hit CCACCGTCGACAACACGCTCTTCACCGGCGACCAGCTCGTGG 5 0.125 No Hit AATGGAAGCCATGCAGAGATCATTTCTAGTGCTGCTCGTGATCTCAGCGGCGATCACGCGTCGG 5 0.125 No Hit AAAAAAGGAGCGTGAGAGCCAAATGAATCGAAAGATTCATGTTTGGTTCGGGAA 5 0.125 No Hit CGTTCATCCGGCTGCTGCGTTCCACGGGCGTCGCCGCCCAAATCGACAAC 5 0.125 No Hit ACACGGATTCCACCGCGTCCCGGGCAGCCGGAGGTGGTGGGGGTGGCGG 5 0.125 No Hit TTGGTCACTTTTAATTTCACAAATGTTTTCTTCCTAGTCAATTTTTCTTT 5 0.125 No Hit GACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACA 5 0.125 No Hit CCGCTCAGGACGCAGGCCGGGAGCCGCTCAACGTCACCGCCACCGGCCAGCA 5 0.125 No Hit AATGGAGCCATGCAGAGATCATTTCTAGTGCTGCTCGTGATCTCAGCGGC 5 0.125 No Hit CCAAATCGACAACCAGCTCAACAGCTCCCAGACGGGCGGGCTCACGGTGT 5 0.125 No Hit TGAGCAACCCGCTCAGGACGCAGGCCGGGAGCAGCTCCCCG 5 0.125 No Hit GTCGACAACACGCTCTTCACCGGCGACCAGCTCGTGGTCTACCAGGTCAA 5 0.125 No Hit CCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATC 5 0.125 No Hit GTATCGTTAATACCGTTGTACAATTGCTAGGGATGTAAGAGTGTAATCTG 5 0.125 No Hit TCACGGCGTCGGCGCAGGGCCCAACGGCGGCGCCAACGA 5 0.125 No Hit TGTTGGAAAGAGAAGGGAGTACCCGCTCAACGTCACCGCCACCGGCCAGCAGGTCAACATC 5 0.125 No Hit TTCCTAGTCAATTTTCTTTGACTTGTTCTCTTCCGTATCGTTAATACCGT 5 0.125 No Hit TCTGCTGGGGGCTGTGACGTTGCCCATGAGACTGCGATTCTCGGTCTACA 5 0.125 No Hit GAAGAAGGCGGACGCGCCGGCCCCCGCGCCGCTGGGTCCGGCCAAGA 5 0.125 No Hit TTTGGTCACTTTTAATTTCACAAATGTTTTCTTCCTAGTCAATTTTTCTT 5 0.125 No Hit AATATACTAATAAGATATTGATCCTTCACAAAGGAACCAGTCCCTCTATGCCACAAAAACATG 5 0.125 No Hit GCGCCCACGGACAACGCGTTCACGAGCCTGGCTTCCGGCACGCTCAACTC 5 0.125 No Hit AGCAGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACACGCTCTTCACCGGCGACCAGCTCGTGG 5 0.125 No Hit ACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTACACCACCAGAATGG 5 0.125 No Hit AATGGAAGCCATGCAGAGATCATTTCTAGTGCTGCTCGTGATCTCAGCGGCGATCACGCGTCGGCGC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-14 0.0 0.0 0.0 0.0 0.0 15-19 0.0 0.0 0.0 0.0 0.0 20-24 0.0 0.0 0.0 0.0 0.0 25-29 0.0 0.0 0.0 0.0 0.0 30-34 0.0 0.0 0.0 0.0 0.0 35-39 0.0 0.0 0.0 0.0 0.0 40-44 0.0 0.0 0.0 0.0 0.0 45-49 0.0 0.0 0.0 0.0 0.0 50-54 0.0 0.0 0.0 0.0 0.0 55-59 0.0 0.0 0.0 0.0 0.0 60-64 0.0 0.0 0.0 0.0 0.0 65-69 0.0 0.0 0.0 0.0 0.0 70-74 0.0 0.0 0.0 0.0 0.0 75-79 0.0 0.0 0.0 0.0 0.0 80-84 0.0 0.0 0.0 0.0 0.0 85-89 0.0 0.0 0.0 0.0 0.0 90-94 0.0 0.0 0.0 0.0 0.0 95-99 0.0 0.0 0.0 0.0 0.0 100-104 0.0 0.0 0.0 0.0 0.0 105-109 0.0 0.0 0.0 0.0 0.0 110-114 0.0 0.0 0.0 0.0 0.0 115-119 0.0 0.0 0.0 0.0 0.0 120-124 0.0 0.0 0.0 0.0 0.0 125-129 0.0 0.0 0.0 0.0 0.0 130-134 0.0 0.0 0.0 0.0 0.0 135-139 0.0 0.0 0.0 0.0 0.0 140-144 0.0 0.0 0.0 0.0 0.0 145-149 0.0 0.0 0.0 0.0 0.0 150-154 0.0 0.0 0.0 0.0 0.0 155-159 0.0 0.0 0.0 0.0 0.0 160-164 0.0 0.0 0.0 0.0 0.0 165-169 0.0 0.0 0.0 0.0 0.0 170-174 0.0 0.0 0.0 0.0 0.0 175-179 0.0 0.0 0.0 0.0 0.0 180-184 0.0 0.0 0.0 0.0 0.0 185-189 0.0 0.0 0.0 0.0 0.0 190-194 0.0 0.0 0.0 0.0 0.0 195-199 0.0 0.0 0.0 0.0 0.0 200-204 0.0 0.0 0.0 0.0 0.0 205-209 0.0 0.0 0.0 0.0 0.0 210-214 0.0 0.0 0.0 0.0 0.0 215-219 0.0 0.0 0.0 0.0 0.0 220-224 0.0 0.0 0.0 0.0 0.0 225-229 0.0 0.0 0.0 0.0 0.0 230-234 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAAATAA 15 0.0016221479 225.96825 150-154 CAGCCCC 15 7.969652E-4 88.975 9 ACACGGA 15 7.969652E-4 88.975 2 CCAGCCC 15 7.969652E-4 88.975 8 CTCCAGC 15 7.969652E-4 88.975 6 GGATTCC 15 7.969652E-4 88.975 6 CGGATTC 15 7.969652E-4 88.975 5 ACTCCAG 15 7.969652E-4 88.975 5 TCCAGCC 15 7.969652E-4 88.975 7 GACACGG 15 7.969652E-4 88.975 1 TTCCACC 15 7.969652E-4 88.975 9 GATTCCA 15 7.969652E-4 88.975 7 ATTCCAC 15 7.969652E-4 88.975 8 GACTCCA 15 7.969652E-4 88.975 4 ACGGATT 15 7.969652E-4 88.975 4 CACGGAT 15 7.969652E-4 88.975 3 AACGACT 20 0.0024973983 66.73125 1 ACGACTC 20 0.0024973983 66.73125 2 CGACTCC 20 0.0024973983 66.73125 3 AAAAAAA 185 8.358256E-9 39.46223 145-149 >>END_MODULE Read 1376689 spots for ERR1942993.sra Written 1376689 spots for ERR1942993.sra Read 1376689 spots for ERR1942993.sra Written 1376689 spots for ERR1942993.sra Read 1376689 spots for ERR1942993.sra Written 1376689 spots for ERR1942993.sra Read 1376689 spots for ERR1942993.sra Written 1376689 spots for ERR1942993.sra Read 1376689 spots for ERR1942993.sra Written 1376689 spots for ERR1942993.sra Read 1376689 spots for ERR1942993.sra Written 1376689 spots for ERR1942993.sra Read 1376689 spots for ERR1942993.sra Written 1376689 spots for ERR1942993.sra Read 1376689 spots for ERR1942993.sra Written 1376689 spots for ERR1942993.sra Read 1376689 spots for ERR1942993.sra Written 1376689 spots for ERR1942993.sra Read 1376689 spots for ERR1942993.sra Written 1376689 spots for ERR1942993.sra Read 1376689 spots for ERR1942993.sra Written 1376689 spots for ERR1942993.sra Read 1376689 spots for ERR1942993.sra Written 1376689 spots for ERR1942993.sra Read 1376689 spots for ERR1942993.sra Written 1376689 spots for ERR1942993.sra Read 1376689 spots for ERR1942993.sra Written 1376689 spots for ERR1942993.sra Read 1376689 spots for ERR1942993.sra Written 1376689 spots for ERR1942993.sra Read 1376689 spots for ERR1942993.sra Written 1376689 spots for ERR1942993.sra Read 1376689 spots for ERR1942993.sra Written 1376689 spots for ERR1942993.sra Read 1376689 spots for ERR1942993.sra Written 1376689 spots for ERR1942993.sra Read 1376692 spots for ERR1942993.sra Written 1376692 spots for ERR1942993.sra Read 1376689 spots for ERR1942993.sra Written 1376689 spots for ERR1942993.sra SRR ids: ['ERR1942993.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_1spqipgb ERR1942993.sra spots: 27533783 blocks: [[1, 1376689], [1376690, 2753378], [2753379, 4130067], [4130068, 5506756], [5506757, 6883445], [6883446, 8260134], [8260135, 9636823], [9636824, 11013512], [11013513, 12390201], [12390202, 13766890], [13766891, 15143579], [15143580, 16520268], [16520269, 17896957], [17896958, 19273646], [19273647, 20650335], [20650336, 22027024], [22027025, 23403713], [23403714, 24780402], [24780403, 26157091], [26157092, 27533783]] ERR1942993 file size 6894954 ERR1942993 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR1942993 ERR1942993_1.fastq Input file: ERR1942993_1.fastq trimmed: ERR1942993-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Tue Dec 10 00:49:50 2024 >> started Tue Dec 10 00:50:06 2024 >> done (16.665s) 27533783 reads processed; of these: 293 ( 0.00%) short reads filtered out after trimming by size control 70 ( 0.00%) empty reads filtered out after trimming by size control 27533420 (100.00%) reads available; of these: 1216242 ( 4.42%) trimmed reads available after processing 26317178 (95.58%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 293 0.00% 19 491 0.00% 20 859 0.00% 21 1403 0.01% 22 1979 0.01% 23 2706 0.01% 24 6365 0.02% 25 38038 0.14% 26 39313 0.14% 27 40156 0.15% 28 41619 0.15% 29 42924 0.16% 30 44481 0.16% 31 46560 0.17% 32 48022 0.17% 33 49464 0.18% 34 51867 0.19% 35 52487 0.19% 36 55373 0.20% 37 57658 0.21% 38 61035 0.22% 39 63395 0.23% 40 66696 0.24% 41 69043 0.25% 42 72688 0.26% 43 75464 0.27% 44 78421 0.28% 45 84714 0.31% 46 86762 0.32% 47 91736 0.33% 48 95746 0.35% 49 99779 0.36% 50 104728 0.38% 51 113840 0.41% 52 113239 0.41% 53 122762 0.45% 54 125649 0.46% 55 130520 0.47% 56 135408 0.49% 57 145907 0.53% 58 148585 0.54% 59 154036 0.56% 60 165946 0.60% 61 168114 0.61% 62 175107 0.64% 63 192553 0.70% 64 188830 0.69% 65 188820 0.69% 66 195295 0.71% 67 204197 0.74% 68 207949 0.76% 69 221521 0.80% 70 223815 0.81% 71 229682 0.83% 72 227417 0.83% 73 230054 0.84% 74 239129 0.87% 75 245308 0.89% 76 248514 0.90% 77 260928 0.95% 78 257292 0.93% 79 257522 0.94% 80 258136 0.94% 81 264878 0.96% 82 270071 0.98% 83 269324 0.98% 84 278524 1.01% 85 280932 1.02% 86 292826 1.06% 87 284446 1.03% 88 282620 1.03% 89 282423 1.03% 90 293032 1.06% 91 290520 1.06% 92 282095 1.02% 93 286442 1.04% 94 287916 1.05% 95 297024 1.08% 96 294663 1.07% 97 290700 1.06% 98 300086 1.09% 99 287372 1.04% 100 289797 1.05% 101 288692 1.05% 102 287321 1.04% 103 280903 1.02% 104 278631 1.01% 105 281217 1.02% 106 270828 0.98% 107 272289 0.99% 108 273702 0.99% 109 277122 1.01% 110 267839 0.97% 111 265441 0.96% 112 263055 0.96% 113 252924 0.92% 114 252351 0.92% 115 252667 0.92% 116 247578 0.90% 117 246286 0.89% 118 245213 0.89% 119 237275 0.86% 120 239797 0.87% 121 229307 0.83% 122 228232 0.83% 123 222591 0.81% 124 220443 0.80% 125 211447 0.77% 126 212121 0.77% 127 205767 0.75% 128 202348 0.73% 129 201293 0.73% 130 197278 0.72% 131 188121 0.68% 132 184238 0.67% 133 180126 0.65% 134 180180 0.65% 135 177226 0.64% 136 174163 0.63% 137 174176 0.63% 138 175965 0.64% 139 167495 0.61% 140 164590 0.60% 141 163216 0.59% 142 152262 0.55% 143 147303 0.53% 144 148479 0.54% 145 145700 0.53% 146 144956 0.53% 147 137098 0.50% 148 135671 0.49% 149 128231 0.47% 150 125262 0.45% 151 122794 0.45% 152 120136 0.44% 153 122596 0.45% 154 124460 0.45% 155 116479 0.42% 156 110497 0.40% 157 105879 0.38% 158 103604 0.38% 159 101893 0.37% 160 99243 0.36% 161 98047 0.36% 162 102691 0.37% 163 99445 0.36% 164 96132 0.35% 165 88748 0.32% 166 84468 0.31% 167 82957 0.30% 168 80758 0.29% 169 77674 0.28% 170 72950 0.26% 171 71822 0.26% 172 70544 0.26% 173 67359 0.24% 174 64806 0.24% 175 62044 0.23% 176 60387 0.22% 177 58983 0.21% 178 58095 0.21% 179 54855 0.20% 180 53101 0.19% 181 50308 0.18% 182 48925 0.18% 183 47301 0.17% 184 46033 0.17% 185 43713 0.16% 186 42003 0.15% 187 41088 0.15% 188 39088 0.14% 189 37345 0.14% 190 35622 0.13% 191 34744 0.13% 192 33083 0.12% 193 31724 0.12% 194 30920 0.11% 195 29285 0.11% 196 28229 0.10% 197 26763 0.10% 198 26025 0.09% 199 24564 0.09% 200 24186 0.09% 201 22880 0.08% 202 22094 0.08% 203 21506 0.08% 204 20421 0.07% 205 19469 0.07% 206 18768 0.07% 207 17478 0.06% 208 16933 0.06% 209 15757 0.06% 210 14991 0.05% 211 14213 0.05% 212 14149 0.05% 213 13684 0.05% 214 13161 0.05% 215 12406 0.05% 216 11761 0.04% 217 10661 0.04% 218 10179 0.04% 219 9279 0.03% 220 8828 0.03% 221 8130 0.03% 222 7473 0.03% 223 7217 0.03% 224 6662 0.02% 225 6274 0.02% 226 5860 0.02% 227 5543 0.02% 228 5154 0.02% 229 4817 0.02% 230 4484 0.02% 231 4202 0.02% 232 3869 0.01% 233 3737 0.01% 234 3377 0.01% 235 3073 0.01% 236 2824 0.01% 237 2666 0.01% 238 2416 0.01% 239 2216 0.01% 240 2069 0.01% 241 1763 0.01% 242 1628 0.01% 243 1484 0.01% 244 1412 0.01% 245 1198 0.00% 246 1093 0.00% 247 1007 0.00% 248 908 0.00% 249 849 0.00% 250 778 0.00% 251 664 0.00% 252 567 0.00% 253 532 0.00% 254 474 0.00% 255 415 0.00% 256 386 0.00% 257 319 0.00% 258 307 0.00% 259 238 0.00% 260 215 0.00% 261 192 0.00% 262 158 0.00% 263 155 0.00% 264 138 0.00% 265 118 0.00% 266 102 0.00% 267 94 0.00% 268 84 0.00% 269 77 0.00% 270 59 0.00% 271 48 0.00% 272 59 0.00% 273 52 0.00% 274 30 0.00% 275 32 0.00% 276 28 0.00% 277 18 0.00% 278 23 0.00% 279 13 0.00% 280 17 0.00% 281 13 0.00% 282 10 0.00% 283 10 0.00% 284 10 0.00% 285 14 0.00% 286 7 0.00% 287 9 0.00% 288 5 0.00% 289 2 0.00% 290 3 0.00% 291 2 0.00% 292 3 0.00% 293 5 0.00% 294 2 0.00% 295 3 0.00% 296 2 0.00% 297 3 0.00% 298 1 0.00% 299 1 0.00% 300 1 0.00% 301 0 0.00% 302 4 0.00% 303 3 0.00% 304 2 0.00% 305 4 0.00% 306 2 0.00% 307 4 0.00% 308 1 0.00% 309 3 0.00% 310 1 0.00% 311 0 0.00% 312 0 0.00% 313 0 0.00% 314 1 0.00% 315 1 0.00% 316 0 0.00% 317 0 0.00% 318 0 0.00% 319 1 0.00% 320 0 0.00% 321 2 0.00% 322 1 0.00% 323 1 0.00% 324 0 0.00% 325 2 0.00% 326 1 0.00% 327 1 0.00% 328 0 0.00% 329 1 0.00% 330 2 0.00% 331 0 0.00% 332 1 0.00% 333 2 0.00% 334 1 0.00% 335 1 0.00% 336 1 0.00% 337 0 0.00% 338 0 0.00% 339 0 0.00% 340 0 0.00% 341 0 0.00% 342 0 0.00% 343 1 0.00% 344 1 0.00% 345 0 0.00% 346 0 0.00% 347 0 0.00% 348 0 0.00% 349 0 0.00% 350 0 0.00% 351 0 0.00% 352 0 0.00% 353 0 0.00% 354 0 0.00% 355 1 0.00% 356 0 0.00% 357 1 0.00% 358 0 0.00% 359 1 0.00% 360 0 0.00% 361 1 0.00% 362 0 0.00% 363 3 0.00% 364 0 0.00% 365 1 0.00% 366 0 0.00% 367 1 0.00% 368 0 0.00% 369 1 0.00% 27533420 reads passed initial QC criterion=sequence-density sequence-density=1.86 sequence-density-rank=1 fanout-score=1.95 fanout-score-rank=38 prefix-density=0.01 prefix-fanout=1.9 sequence=ACAGCTATCACACACAGGCAAAACACAGCTGATTCGTGTACTCGATCTCCCCAGCAAGTTAAGGCCACCATGTCGAGCGGCTGCGGCAACTGCGACTGCGCTGACAAGACCCAGTGTGTGAAGAAGGGAAACGGCTACGGCATCGTCATGGTTGACACCGAGAAGAGCCACTTCGAGGTGCAGGAGTCCGCGGCGGAGAACGACGGCAAGTGCAAGTGCGGCACCAGCTGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=42.47 fanout-score-rank=1 prefix-density=0.13 prefix-fanout=3.9 sequence=CATCTCCTCCAGAGATTAAGGGTTTGATAAAGGATAAGAAGACAGCCAAGATCAAGAAGAACTCTGTTGCAGTCACCAAGTTCCTTGATGATT Started job on | Dec 10 00:50:20 Started mapping on | Dec 10 00:50:21 Finished on | Dec 10 00:51:03 Mapping speed, Million of reads per hour | 2360.01 Number of input reads | 27533420 Average input read length | 106 UNIQUE READS: Uniquely mapped reads number | 23395984 Uniquely mapped reads % | 84.97% Average mapped length | 104.97 Number of splices: Total | 7288056 Number of splices: Annotated (sjdb) | 6673840 Number of splices: GT/AG | 6966667 Number of splices: GC/AG | 87490 Number of splices: AT/AC | 5272 Number of splices: Non-canonical | 228627 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.25% Deletion average length | 1.17 Insertion rate per base | 0.21% Insertion average length | 1.17 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1207765 % of reads mapped to multiple loci | 4.39% Number of reads mapped to too many loci | 608404 % of reads mapped to too many loci | 2.21% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 7.49% % of reads unmapped: other | 0.94% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2929671 2929671 2929671 N_multimapping 1207765 1207765 1207765 N_noFeature 920827 1091591 22755309 N_ambiguous 537069 77283 2376 UnstrandedReadsAssigned:21938088 PositiveStrandReadsAssigned:22227110 NegativeStrandReadsAssigned:638299 Dataset is classified positive stranded MeadianReadLen=102 20thPercentileLength=74 echo kmer=69 ERR1942993 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR1942993-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 27,533,420 reads, 23,159,626 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,186 rounds 52973 ERR1942993.ke.tsv 35125 ERR1942993.se.tsv 88098 total ==> ERR1942993.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 131.769 9.90225 PNS24247 1044 945 20.4167 1.35894 PNS24249 1928 1829 0 0 PNS24246 1044 945 20.4167 1.35894 PNS24248 1044 945 20.4167 1.35894 PNS24244 1471 1372 217.981 9.99338 PNS24243 293 194 0 0 KQK14069 1603 1504 8428.39 352.488 KQK14071 474 375 149.317 25.0453 ==> ERR1942993.se.tsv <== BRADI_1g14170v3 8799 BRADI_1g53295v3 101 BRADI_1g59795v3 611 BRADI_1g07683v3 0 BRADI_1g00485v3 15 BRADI_1g20270v3 677 BRADI_1g74790v3 508 BRADI_1g09890v3 2 BRADI_1g77505v3 729 BRADI_1g48960v3 0 ERR1942993 completed mapping pipeline successfully