Starting /dee2/code/volunteer_pipeline.sh ERR1942996 current disk space = 1523551387648 free memory = 1566185480 ERR1942996 SRAfilesize 9f7fb1cb51b64bec4afa785c73da8e8c ERR1942996.sra ERR1942996.sra file validated ERR1942996 is single end ERR1942996 is conventional basespace ERR1942996 read1 length is 25-247 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1942996_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 25-247 %GC 59 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.1165 26.0 23.0 27.0 14.0 28.0 2 22.841 24.0 20.0 27.0 14.0 28.0 3 21.83525 23.0 18.0 27.0 14.0 28.0 4 22.9385 24.0 20.0 27.0 14.0 28.0 5 21.419 23.0 17.0 27.0 14.0 28.0 6 21.9755 23.0 19.0 27.0 14.0 28.0 7 23.06925 24.0 21.0 27.0 14.0 28.0 8 23.2285 24.0 21.0 27.0 14.0 28.0 9 22.78625 24.0 20.0 27.0 14.0 28.0 10-14 24.0245 24.8 21.4 27.6 17.6 28.8 15-19 25.138450000000002 26.8 23.4 28.0 19.0 29.0 20-24 24.75955 26.2 22.8 27.8 19.6 28.8 25-29 24.41131698559414 25.4 22.4 27.8 19.0 28.6 30-34 24.449750211104856 25.8 22.2 27.6 18.8 28.8 35-39 24.82026989906408 26.0 23.2 27.8 20.0 28.8 40-44 24.67843628186838 25.8 22.8 27.8 19.8 28.2 45-49 24.43523069847195 25.4 22.4 27.6 19.2 28.6 50-54 24.29605882891237 25.4 22.4 27.6 18.4 28.6 55-59 24.169144214926312 25.6 22.2 27.6 17.4 28.6 60-64 23.828656981724567 25.2 22.0 27.4 15.6 28.4 65-69 22.693334599314436 24.0 20.4 26.8 13.6 28.0 70-74 21.973587477417958 23.4 17.6 27.0 12.4 28.0 75-79 22.18903601026569 23.2 19.4 26.8 13.4 28.0 80-84 20.692392808695658 22.2 15.0 25.8 10.8 28.0 85-89 20.63956731046553 21.6 15.6 25.4 12.2 27.4 90-94 20.704736321445367 21.8 17.4 24.6 12.8 27.0 95-99 21.573987620000757 22.8 19.4 25.6 13.0 27.6 100-104 21.398101997897193 22.8 19.0 25.8 13.0 27.4 105-109 21.17881907054722 22.0 18.4 25.4 13.0 27.2 110-114 21.074623469360326 22.0 18.6 25.0 12.8 27.0 115-119 20.267680123195596 21.4 17.4 24.8 11.8 26.8 120-124 20.052276477025003 21.0 15.6 24.0 12.0 26.4 125-129 19.627148254585144 20.4 14.0 24.0 11.0 26.2 130-134 19.581290917463626 20.4 14.0 24.0 10.4 26.2 135-139 19.827791325949867 21.0 14.4 24.0 11.6 26.0 140-144 19.61633910805393 20.4 14.0 23.4 12.4 25.6 145-149 20.261818674607895 21.6 17.6 23.8 13.4 25.8 150-154 20.218177463605112 21.0 17.0 24.0 13.4 25.4 155-159 19.906836200682385 20.4 15.6 23.6 13.6 25.8 160-164 19.31836872844848 20.0 14.0 23.2 12.4 25.6 165-169 19.46601066693131 20.2 14.2 23.8 12.4 25.6 170-174 19.717897225055317 20.6 15.6 23.6 13.0 25.4 175-179 19.907951075468876 20.8 17.2 23.4 12.6 25.2 180-184 19.174153447774728 20.0 14.8 23.0 12.4 25.0 185-189 19.792233976232385 20.4 16.6 23.4 12.4 25.4 190-194 19.12939761000634 20.2 14.0 23.0 11.8 25.0 195-199 19.454591409812465 20.2 14.8 24.0 11.6 25.0 200-204 19.718063963177535 20.2 15.0 23.8 12.4 25.0 205-209 19.538760657299356 20.0 14.0 23.0 12.0 25.0 210-214 18.096458194179714 NaN NaN NaN NaN NaN 215-219 19.09867830279868 NaN NaN NaN NaN NaN 220-224 18.64445054945055 NaN NaN NaN NaN NaN 225-229 18.998809523809523 NaN NaN NaN NaN NaN 230-234 18.006699346405227 NaN NaN NaN NaN NaN 235-239 18.22687312687313 NaN NaN NaN NaN NaN 240-244 18.478571428571428 NaN NaN NaN NaN NaN 245-247 11.333333333333334 NaN NaN NaN NaN NaN >>END_MODULE >>Per sequence quality scores warn #Quality Count 15 2.0 16 8.0 17 37.0 18 105.0 19 202.0 20 344.0 21 513.0 22 480.0 23 531.0 24 596.0 25 554.0 26 417.0 27 207.0 28 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 8.95 76.0 6.0 9.049999999999999 2 15.25 40.45 3.0 41.3 3 10.274999999999999 22.3 10.549999999999999 56.875 4 28.000000000000004 48.85 8.475000000000001 14.674999999999999 5 11.774999999999999 55.974999999999994 8.025 24.224999999999998 6 15.174999999999999 28.249999999999996 11.450000000000001 45.125 7 15.049999999999999 42.575 25.5 16.875 8 11.450000000000001 20.95 30.2 37.4 9 10.8 41.625 9.049999999999999 38.525 10-14 29.205 26.490000000000002 10.489999999999998 33.815 15-19 34.144999999999996 18.54 15.82 31.495 20-24 26.755000000000003 21.77 18.985 32.49 25-29 28.933400100150227 31.321982974461694 9.469203805708563 30.275413119679516 30-34 38.67297623842126 19.557994361659283 10.617196939186469 31.151832460732987 35-39 29.182702045776786 21.207210856795626 21.586996151509013 28.02309094591857 40-44 17.29837269805642 36.71376830077029 15.354792633780544 30.633066367392747 45-49 22.362409138110074 24.922118380062305 22.191069574247145 30.524402907580477 50-54 29.275594760759155 20.518577920342153 15.129644480085538 35.07618283881315 55-59 21.82741116751269 24.26281868476587 17.760793931443565 36.14897621627788 60-64 24.254401724757457 24.853275841418135 24.685591088753146 26.206731345071265 65-69 33.08922721140875 17.74908460204278 13.323055180831245 35.83863300571723 70-74 34.365346330747585 12.665614059174848 19.585364179309398 33.38367543076817 75-79 27.670527670527672 23.166023166023166 10.87137557725793 38.292073586191236 80-84 24.7911794186435 25.13364517206816 12.654527230203808 37.42064817908453 85-89 25.392595412582086 25.649566955363092 16.598458170743314 32.359379461311505 90-94 13.889787555267983 32.103957726733526 13.857435565620618 40.148819152377875 95-99 17.893733619251847 25.053609721229446 21.622587562544673 35.43006909697403 100-104 16.547807712625463 24.590596936080296 17.15530903328051 41.70628631801374 105-109 26.791033077284844 19.517165699952972 11.631917228405706 42.05988399435648 110-114 36.84009181331293 17.463657230298395 13.274674827850038 32.42157612853864 115-119 28.77388876959416 17.736740390809533 16.16920764440627 37.320163195190034 120-124 34.89422391252674 14.35702400760637 15.355360114095554 35.39339196577133 125-129 34.465638881630525 9.511366605696368 21.19153383851581 34.831460674157306 130-134 27.294117647058826 13.352941176470587 10.294117647058822 49.05882352941177 135-139 23.39733159778718 28.538887081028314 9.046534331272372 39.01724698991214 140-144 27.353342428376536 26.159618008185536 10.504774897680765 35.982264665757164 145-149 25.979159180740208 17.67876392382321 18.720804886812793 37.621272008623784 150-154 27.050820328131252 23.04921968787515 13.925570228091235 35.97438975590236 155-159 29.183049366535606 11.708169506334643 20.751419833988642 38.35736129314111 160-164 30.632318501170957 12.177985948477751 15.269320843091336 41.92037470725995 165-169 32.939362795477905 15.878725590955806 11.97327852004111 39.20863309352518 170-174 20.989668297988036 21.315932572050027 16.748232735182164 40.94616639477977 175-179 21.89957652752571 13.853599516031458 27.223230490018146 37.02359346642468 180-184 28.092105263157897 11.513157894736842 26.57894736842105 33.81578947368421 185-189 29.52815829528158 19.863013698630137 12.32876712328767 38.28006088280061 190-194 26.304579339723112 24.920127795527154 13.525026624068156 35.250266240681576 195-199 26.686656671664167 23.238380809595203 13.793103448275861 36.28185907046477 200-204 25.800711743772244 20.106761565836297 19.03914590747331 35.05338078291815 205-209 25.792811839323466 23.044397463002113 15.644820295983086 35.517970401691336 210-214 27.92022792022792 11.396011396011396 28.205128205128204 32.47863247863248 215-219 31.226765799256505 16.356877323420075 14.12639405204461 38.28996282527881 220-224 24.375 24.375 11.25 40.0 225-229 19.130434782608695 25.217391304347824 16.52173913043478 39.130434782608695 230-234 23.170731707317074 14.634146341463413 21.951219512195124 40.243902439024396 235-239 27.419354838709676 17.741935483870968 20.967741935483872 33.87096774193548 240-244 47.61904761904761 14.285714285714285 4.761904761904762 33.33333333333333 245-247 33.33333333333333 0.0 0.0 66.66666666666666 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 1.0 16 1.0 17 0.5 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.5 24 0.5 25 0.5 26 1.5 27 2.0 28 2.0 29 1.0 30 0.0 31 0.5 32 2.0 33 7.5 34 33.5 35 48.5 36 40.5 37 37.0 38 35.5 39 33.0 40 29.5 41 33.5 42 31.0 43 17.0 44 66.0 45 123.0 46 122.0 47 92.5 48 75.5 49 77.0 50 63.0 51 47.5 52 50.0 53 82.0 54 156.5 55 215.5 56 155.0 57 85.0 58 91.5 59 162.5 60 379.5 61 485.5 62 367.33333333333326 63 407.99999999999983 64 364.49999999999926 65 222.8333333333335 66 198.3333333333335 67 144.49999999999997 68 114.16666666666666 69 88.5 70 76.0 71 64.5 72 38.5 73 22.0 74 20.0 75 13.5 76 7.5 77 8.5 78 11.0 79 10.0 80 5.5 81 1.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-154 0.0 155-159 0.0 160-164 0.0 165-169 0.0 170-174 0.0 175-179 0.0 180-184 0.0 185-189 0.0 190-194 0.0 195-199 0.0 200-204 0.0 205-209 0.0 210-214 0.0 215-219 0.0 220-224 0.0 225-229 0.0 230-234 0.0 235-239 0.0 240-244 0.0 245-247 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 25-29 19.0 30-34 22.0 35-39 26.0 40-44 34.0 45-49 86.0 50-54 252.0 55-59 142.0 60-64 212.0 65-69 210.0 70-74 241.0 75-79 265.0 80-84 235.0 85-89 323.0 90-94 186.0 95-99 166.0 100-104 178.0 105-109 287.0 110-114 159.0 115-119 81.0 120-124 84.0 125-129 82.0 130-134 71.0 135-139 45.0 140-144 21.0 145-149 52.0 150-154 54.0 155-159 24.0 160-164 43.0 165-169 21.0 170-174 36.0 175-179 29.0 180-184 28.0 185-189 71.0 190-194 68.0 195-199 29.0 200-204 16.0 205-209 23.0 210-214 17.0 215-219 23.0 220-224 15.0 225-229 6.0 230-234 4.0 235-239 6.0 240-244 7.0 245-248 1.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.075000000000003 #Duplication Level Percentage of deduplicated Percentage of total 1 75.31172069825436 22.650000000000002 2 7.065669160432253 4.25 3 4.57190357439734 4.125 4 3.0756442227763925 3.6999999999999997 5 1.4131338320864506 2.125 6 1.4131338320864506 2.55 7 0.8312551953449709 1.7500000000000002 8 1.1637572734829593 2.8000000000000003 9 0.3325020781379884 0.8999999999999999 >10 4.239401496259352 23.125 >50 0.24937655860349126 5.7 >100 0.3325020781379884 26.325 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCAT 432 10.8 No Hit AACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGA 389 9.725 No Hit ACCACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATC 119 2.9749999999999996 No Hit AACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCG 113 2.825 No Hit ACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCG 93 2.325 No Hit TAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGGCTG 84 2.1 No Hit ACCAAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCA 51 1.275 No Hit AAAAAAGGAGCGTGAGAGCCAAATGAATCGAAAGATTCATGTTTGGTTCG 38 0.95 No Hit AATGGAGCCATGCAGAGATCATTTCTAGTGCTGCTCGTGATCTCAGCGGC 37 0.9249999999999999 No Hit AAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACAC 36 0.8999999999999999 No Hit ACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGAC 35 0.8750000000000001 No Hit AGATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGAT 33 0.8250000000000001 No Hit ATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTA 32 0.8 No Hit AACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGT 28 0.7000000000000001 No Hit GAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAG 25 0.625 No Hit CAAGCTCTCCACCAGATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTACACCACCAG 25 0.625 No Hit GATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATT 24 0.6 No Hit GGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCA 24 0.6 No Hit CCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATC 24 0.6 No Hit AGATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTACACCACCAG 21 0.525 No Hit AACGACTCCAGCCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAG 21 0.525 No Hit ACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATC 20 0.5 No Hit CAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACATAACA 19 0.475 No Hit AATGGAAGCCATGCAGAGATCATTTCTAGTGCTGCTCGTGATCTCAGCGG 18 0.44999999999999996 No Hit ACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTT 18 0.44999999999999996 No Hit AATCTTGTTATAACTAAGCCACAGCTAAGCTCGCAAGCTCTCCACCAGAT 18 0.44999999999999996 No Hit ACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTT 17 0.42500000000000004 No Hit AAGAAAAAAGGAGCGTGAGAGCCAAATGAATCGAAAGATTCATGTTTGGT 17 0.42500000000000004 No Hit ACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGAC 17 0.42500000000000004 No Hit GCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGC 17 0.42500000000000004 No Hit GAAAAAAGGAGCGTGAGAGCCAAATGAATCGAAAGATTCATGTTTGGTTC 17 0.42500000000000004 No Hit GGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCACGGGCGTCGCCGCC 16 0.4 No Hit CGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGC 16 0.4 No Hit AGTGCTGCTCGTGATCTCAGCGGCGATCACGGCGTCGGCGCAGGGCCCAACGGCGGCGCCAACGACTCC 16 0.4 No Hit ACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACG 16 0.4 No Hit AGAAAAAAGGAGCGTGAGAGCCAAATGAATCGAAAGATTCATGTTTGGTT 15 0.375 No Hit AACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACA 15 0.375 No Hit ACCAACATAGCAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCAT 15 0.375 No Hit TCATTTCTAGTGCTGCTCGTGATCTCAGCGGCGATCACGGCGTCGGCGCA 15 0.375 No Hit ACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCACGG 14 0.35000000000000003 No Hit AGTGCTGCTCGTGATCTCAGCGGCGATCACGCGTCGGCGCAGGGCCCAACGG 13 0.325 No Hit ACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCC 13 0.325 No Hit ACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGG 13 0.325 No Hit AATGGAAGCCATGCAGAGATCATTTCTAGTGCTGCTCGTGATCTCAGCGGCGATCACGCGTCGG 12 0.3 No Hit AGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGGC 12 0.3 No Hit GCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTACACCACCAGAATG 12 0.3 No Hit ACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGA 12 0.3 No Hit AACACGTTCATCCGGCTGCTGCGTTCCACGGGCGTCGCCGCCCAAATCGA 12 0.3 No Hit ACAGCTAAGCTCGCAAGCTCTCCACCAGATAGATCACTCTCGCATTTGTC 11 0.27499999999999997 No Hit ACCAGATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTACACCACCAG 11 0.27499999999999997 No Hit CACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCG 11 0.27499999999999997 No Hit CAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACA 11 0.27499999999999997 No Hit AAGACGCCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACG 11 0.27499999999999997 No Hit ACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCC 11 0.27499999999999997 No Hit CATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTACACCACCAGAATGGAGCCATGCAGAGATCATTTCT 11 0.27499999999999997 No Hit AGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCG 10 0.25 No Hit GACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGA 10 0.25 No Hit GGAACGACCAAGACGCCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAG 10 0.25 No Hit GCACCGGCAGCCGGAACGACCAAGACGCCAACATAACAGGCGTGCTAGCG 9 0.22499999999999998 No Hit ATCTCACTCCAATCTTGTTATAACTAAGCCACAGCTAAGCTCGCAAGCTC 9 0.22499999999999998 No Hit ACCACACACACAAACCCTGCTGCACCCACCACCAATGCTACGTGCTCTGC 9 0.22499999999999998 No Hit ATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTACACC 9 0.22499999999999998 No Hit ACCAACATACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATC 8 0.2 No Hit ACCAAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCG 8 0.2 No Hit CAAGCTCTCCACCAGATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTACACCACC 8 0.2 No Hit AGATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTACACCACCAGAATGGAAGCCATGC 8 0.2 No Hit CCACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCC 8 0.2 No Hit ACCACATAAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCAT 8 0.2 No Hit ACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGC 8 0.2 No Hit TCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTACACCACCAGAATGGAAGCCATGC 8 0.2 No Hit ACCAGATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCAC 8 0.2 No Hit AAGGCGGGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCACGGGCGTC 8 0.2 No Hit AACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACC 8 0.2 No Hit AACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGACGACCAAGAC 8 0.2 No Hit GGAACCAATCTGATGAGTTGCAATTTGACACCAACTTCTGTCGTACACTC 8 0.2 No Hit AGTGCTGCTCGTGATCTCAGCGGCGATCACGGCGTCGGCGCAGGGCCCAACGG 8 0.2 No Hit AAGGCGGGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCACGGCGTCG 7 0.17500000000000002 No Hit AACGACTCCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAG 7 0.17500000000000002 No Hit GACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCA 7 0.17500000000000002 No Hit AACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGCCAACATAACAGGCGTGCTAGCG 7 0.17500000000000002 No Hit AGTGCTGCTCGTGATCTCAGCGGCGATCACGGCGTCGGCGCAGGGCCCAACGGCGGCGCCAACGA 7 0.17500000000000002 No Hit AGATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTACACCACCAGAATGGAAGCCA 7 0.17500000000000002 No Hit AAGAAAAAAGGAGCGTGAGAGCCAAATGAATCGAAAGATTCATGTTTGGTTCGGGAAGAGATCATAAAAGTTG 7 0.17500000000000002 No Hit ATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTACACCACCAG 7 0.17500000000000002 No Hit AGTGCTGCTCGTGATCTCAGCGGCGATCACGGCGTCGGCGCAGGGCCCAACGGCGGCGCCAACG 7 0.17500000000000002 No Hit ACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCACGGCGTC 7 0.17500000000000002 No Hit AAAAAAGGAGCGTGAGAGCCAAATGAATCGAAAGAATTCATGTTTGGTTC 6 0.15 No Hit TAAGCCACAGCTAAGCTCGCAAGCTCTCCACCAGATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAG 6 0.15 No Hit CAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCACGGG 6 0.15 No Hit AGCAATCAAAAACTAATTTCTCCATTTTCGAACATCTTCAAATGTTAGCC 6 0.15 No Hit ACCGGCAGCCGGAACGACCAAGACGCCAACATAACAGGCGTGCTAGCGAA 6 0.15 No Hit CCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGG 6 0.15 No Hit GACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACA 6 0.15 No Hit AGATAGATCACTCTCGCATTTGCCCTCGCTGATAGCTAGAGTTTCACGAT 6 0.15 No Hit ACTCCAATCTTGTTATAACTAAGCCACAGCTAAGCTCGCAAGCTCTCCAC 6 0.15 No Hit CCCAAATCGACAACCAGCTCAACAGCTCCCAGACGGGCGGGCTCACGGTG 6 0.15 No Hit ACCCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCA 6 0.15 No Hit ACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGG 6 0.15 No Hit AAGAAAAAAGGAGCGTGAGAGCCAAAATGAATCGAAAGATTCATGTTTGG 6 0.15 No Hit ACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGGC 6 0.15 No Hit GGCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAG 6 0.15 No Hit AATGGAAGCCATGCAGAGATCATTTCTAGTGCTGCTCGTGATCTCAGCGGCGATCACGGCGTCGG 6 0.15 No Hit CCAGATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTACACCACCAG 6 0.15 No Hit AGCGAAAGCCGGGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCACGG 5 0.125 No Hit AGTGCTGCTCGTGATCTCAGCGGCGATCACGGCGTCGGCGCAGGGCCCAACGGCGGCGCC 5 0.125 No Hit GAATGGAAGCCATGCAGAGATCATTTCTAGTGCTGCTCGTGATCTCAGCGGCGATCACGCGTCGG 5 0.125 No Hit CCAACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACA 5 0.125 No Hit TCCACGGGCGTCGCCGCCCAAATCGACAACCAGCTCAACAGCTCCCAGAC 5 0.125 No Hit ACGTTCATCCGGCTGCTGCGTTCCACGGGCGTCGCCGCCCAAATCGACAA 5 0.125 No Hit AATGGAGCCATGCAGAGATCATTTCTAGTGCTGCTCGTGATCTCAGCGGCGATCACGCGTCGG 5 0.125 No Hit TTCTAGAAGACCAAAGTGTTTTGCTGTTGTTACGACTTTCAGCTGAGGAG 5 0.125 No Hit CGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTACACCACCAGAATGGAAGCCA 5 0.125 No Hit ACCAACATAAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCA 5 0.125 No Hit AAGGGAACCAATCTGATGAGTTGCAATTTGACACCAACTTCTGTCGTACA 5 0.125 No Hit AGACGCCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGGCTGCTGCG 5 0.125 No Hit ACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAAC 5 0.125 No Hit CACCGGCAGCCGGAACGACCAAGACGCCAACATAACAGGCGTGCTAGCGA 5 0.125 No Hit ACTCCAGCCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGA 5 0.125 No Hit AGTTCAACACGTTCATCCGGCTGCTGCGTTCCACGGGCGTCGCCGCCCAA 5 0.125 No Hit ACCACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-14 0.0 0.0 0.0 0.0 0.0 15-19 0.0 0.0 0.0 0.0 0.0 20-24 0.0 0.0 0.0 0.0 0.0 25-29 0.0 0.0 0.0 0.0 0.0 30-34 0.0 0.0 0.0 0.0 0.0 35-39 0.0 0.0 0.0 0.0 0.0 40-44 0.0 0.0 0.0 0.0 0.0 45-49 0.0 0.0 0.0 0.0 0.0 50-54 0.0 0.0 0.0 0.0 0.0 55-59 0.0 0.0 0.0 0.0 0.0 60-64 0.0 0.0 0.0 0.0 0.0 65-69 0.0 0.0 0.0 0.0 0.0 70-74 0.0 0.0 0.0 0.0 0.0 75-79 0.0 0.0 0.0 0.0 0.0 80-84 0.0 0.0 0.0 0.0 0.0 85-89 0.0 0.0 0.0 0.0 0.0 90-94 0.0 0.0 0.0 0.0 0.0 95-99 0.0 0.0 0.0 0.0 0.0 100-104 0.0 0.0 0.0 0.0 0.0 105-109 0.0 0.0 0.0 0.0 0.0 110-114 0.0 0.0 0.0 0.0 0.0 115-119 0.0 0.0 0.0 0.0 0.0 120-124 0.0 0.0 0.0 0.0 0.0 125-129 0.0 0.0 0.0 0.0 0.0 130-134 0.0 0.0 0.0 0.0 0.0 135-139 0.0 0.0 0.0 0.0 0.0 140-144 0.0 0.0 0.0 0.0 0.0 145-149 0.0 0.0 0.0 0.0 0.0 150-154 0.0 0.0 0.0 0.0 0.0 155-159 0.0 0.0 0.0 0.0 0.0 160-164 0.0 0.0 0.0 0.0 0.0 165-169 0.0 0.0 0.0 0.0 0.0 170-174 0.0 0.0 0.0 0.0 0.0 175-179 0.0 0.0 0.0 0.0 0.0 180-184 0.0 0.0 0.0 0.0 0.0 185-189 0.0 0.0 0.0 0.0 0.0 190-194 0.0 0.0 0.0 0.0 0.0 195-199 0.0 0.0 0.0 0.0 0.0 200-204 0.0 0.0 0.0 0.0 0.0 205-209 0.0 0.0 0.0 0.0 0.0 210-214 0.0 0.0 0.0 0.0 0.0 215-219 0.0 0.0 0.0 0.0 0.0 220-224 0.0 0.0 0.0 0.0 0.0 225-229 0.0 0.0 0.0 0.0 0.0 230-234 0.0 0.0 0.0 0.0 0.0 235 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACAGC 10 0.0011722633 1869.25 205-209 TCGCACG 5 0.0029298745 1495.3999 210-214 CGCACGC 5 0.0029298745 1495.3999 215-219 CTCGCAC 5 0.0029298745 1495.3999 210-214 TGGCTCG 5 0.0029298745 1495.3999 210-214 CTGGCTC 5 0.00439442 1246.1666 205-209 GCTCGCA 15 0.0079106605 830.7778 210-214 TCAACTC 45 0.002200503 207.69444 220-223 CTCAACT 50 2.9490137E-5 186.925 220-223 ACGGCGG 25 0.0033083174 175.92941 110-114 ACGCTCA 50 0.0033420958 149.54001 215-219 CACGCTC 50 4.436829E-5 149.54001 215-219 GCACGCT 50 4.436829E-5 149.54001 215-219 GGACACG 10 0.006088212 149.54 135-139 GACACGC 10 0.006088212 149.54 135-139 GAGCCTG 45 0.0075778356 138.46295 205-209 CCTGGCT 45 4.859113E-5 138.46295 205-209 CGTCGCG 10 0.0086001195 133.51787 120-124 CGCCACG 25 0.0017718762 106.81429 185-189 TCACGAG 35 0.002800522 106.814285 200-204 >>END_MODULE Read 1653745 spots for ERR1942996.sra Written 1653745 spots for ERR1942996.sra Read 1653745 spots for ERR1942996.sra Written 1653745 spots for ERR1942996.sra Read 1653745 spots for ERR1942996.sra Written 1653745 spots for ERR1942996.sra Read 1653745 spots for ERR1942996.sra Written 1653745 spots for ERR1942996.sra Read 1653745 spots for ERR1942996.sra Written 1653745 spots for ERR1942996.sra Read 1653745 spots for ERR1942996.sra Written 1653745 spots for ERR1942996.sra Read 1653745 spots for ERR1942996.sra Written 1653745 spots for ERR1942996.sra Read 1653745 spots for ERR1942996.sra Written 1653745 spots for ERR1942996.sra Read 1653745 spots for ERR1942996.sra Written 1653745 spots for ERR1942996.sra Read 1653745 spots for ERR1942996.sra Written 1653745 spots for ERR1942996.sra Read 1653745 spots for ERR1942996.sra Written 1653745 spots for ERR1942996.sra Read 1653745 spots for ERR1942996.sra Written 1653745 spots for ERR1942996.sra Read 1653745 spots for ERR1942996.sra Written 1653745 spots for ERR1942996.sra Read 1653745 spots for ERR1942996.sra Written 1653745 spots for ERR1942996.sra Read 1653745 spots for ERR1942996.sra Written 1653745 spots for ERR1942996.sra Read 1653745 spots for ERR1942996.sra Written 1653745 spots for ERR1942996.sra Read 1653748 spots for ERR1942996.sra Written 1653748 spots for ERR1942996.sra Read 1653745 spots for ERR1942996.sra Written 1653745 spots for ERR1942996.sra Read 1653745 spots for ERR1942996.sra Written 1653745 spots for ERR1942996.sra Read 1653745 spots for ERR1942996.sra Written 1653745 spots for ERR1942996.sra SRR ids: ['ERR1942996.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_8x81srkn ERR1942996.sra spots: 33074903 blocks: [[1, 1653745], [1653746, 3307490], [3307491, 4961235], [4961236, 6614980], [6614981, 8268725], [8268726, 9922470], [9922471, 11576215], [11576216, 13229960], [13229961, 14883705], [14883706, 16537450], [16537451, 18191195], [18191196, 19844940], [19844941, 21498685], [21498686, 23152430], [23152431, 24806175], [24806176, 26459920], [26459921, 28113665], [28113666, 29767410], [29767411, 31421155], [31421156, 33074903]] ERR1942996 file size 8009760 ERR1942996 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR1942996 ERR1942996_1.fastq Input file: ERR1942996_1.fastq trimmed: ERR1942996-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Tue Dec 10 00:48:40 2024 >> started Tue Dec 10 00:48:59 2024 >> done (18.854s) 33074903 reads processed; of these: 387 ( 0.00%) short reads filtered out after trimming by size control 41 ( 0.00%) empty reads filtered out after trimming by size control 33074475 (100.00%) reads available; of these: 1375232 ( 4.16%) trimmed reads available after processing 31699243 (95.84%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 442 0.00% 19 613 0.00% 20 1119 0.00% 21 1919 0.01% 22 2347 0.01% 23 3086 0.01% 24 7464 0.02% 25 38859 0.12% 26 40190 0.12% 27 42817 0.13% 28 42967 0.13% 29 43907 0.13% 30 46047 0.14% 31 46600 0.14% 32 47405 0.14% 33 49633 0.15% 34 50569 0.15% 35 52733 0.16% 36 55802 0.17% 37 57306 0.17% 38 60040 0.18% 39 64150 0.19% 40 66863 0.20% 41 69949 0.21% 42 76093 0.23% 43 78497 0.24% 44 83334 0.25% 45 91401 0.28% 46 95721 0.29% 47 101057 0.31% 48 109828 0.33% 49 114712 0.35% 50 120564 0.36% 51 134669 0.41% 52 136534 0.41% 53 150364 0.45% 54 155327 0.47% 55 163677 0.49% 56 173537 0.52% 57 188467 0.57% 58 191494 0.58% 59 202723 0.61% 60 215127 0.65% 61 221991 0.67% 62 233294 0.71% 63 257422 0.78% 64 252661 0.76% 65 257859 0.78% 66 265697 0.80% 67 280395 0.85% 68 288768 0.87% 69 303961 0.92% 70 314670 0.95% 71 330058 1.00% 72 327489 0.99% 73 330089 1.00% 74 338360 1.02% 75 344364 1.04% 76 350275 1.06% 77 376075 1.14% 78 372714 1.13% 79 367066 1.11% 80 370153 1.12% 81 372456 1.13% 82 377629 1.14% 83 376409 1.14% 84 390716 1.18% 85 388059 1.17% 86 394712 1.19% 87 390839 1.18% 88 391236 1.18% 89 396914 1.20% 90 422331 1.28% 91 397553 1.20% 92 390187 1.18% 93 393708 1.19% 94 391451 1.18% 95 399237 1.21% 96 402557 1.22% 97 394740 1.19% 98 405118 1.22% 99 380317 1.15% 100 379051 1.15% 101 376290 1.14% 102 373310 1.13% 103 364153 1.10% 104 357054 1.08% 105 363277 1.10% 106 351248 1.06% 107 345949 1.05% 108 338567 1.02% 109 343136 1.04% 110 334845 1.01% 111 329607 1.00% 112 319386 0.97% 113 307724 0.93% 114 310240 0.94% 115 312886 0.95% 116 300863 0.91% 117 296173 0.90% 118 292287 0.88% 119 282601 0.85% 120 279292 0.84% 121 270373 0.82% 122 264216 0.80% 123 257658 0.78% 124 248486 0.75% 125 243837 0.74% 126 245373 0.74% 127 235139 0.71% 128 227073 0.69% 129 224550 0.68% 130 218695 0.66% 131 207175 0.63% 132 204518 0.62% 133 197607 0.60% 134 193747 0.59% 135 189380 0.57% 136 182141 0.55% 137 177906 0.54% 138 177204 0.54% 139 168896 0.51% 140 165500 0.50% 141 157949 0.48% 142 150325 0.45% 143 146132 0.44% 144 145054 0.44% 145 143395 0.43% 146 143130 0.43% 147 133435 0.40% 148 128661 0.39% 149 123531 0.37% 150 121503 0.37% 151 115514 0.35% 152 112905 0.34% 153 112242 0.34% 154 113630 0.34% 155 110667 0.33% 156 102579 0.31% 157 100468 0.30% 158 95411 0.29% 159 96888 0.29% 160 93559 0.28% 161 91985 0.28% 162 93700 0.28% 163 92011 0.28% 164 85410 0.26% 165 79438 0.24% 166 73333 0.22% 167 71460 0.22% 168 69011 0.21% 169 66547 0.20% 170 64535 0.20% 171 62272 0.19% 172 58533 0.18% 173 55844 0.17% 174 53812 0.16% 175 51547 0.16% 176 50239 0.15% 177 50157 0.15% 178 48321 0.15% 179 45129 0.14% 180 44198 0.13% 181 42304 0.13% 182 40670 0.12% 183 38841 0.12% 184 37069 0.11% 185 35538 0.11% 186 34339 0.10% 187 33313 0.10% 188 31564 0.10% 189 29804 0.09% 190 28645 0.09% 191 27399 0.08% 192 27152 0.08% 193 26249 0.08% 194 25739 0.08% 195 24499 0.07% 196 22673 0.07% 197 22099 0.07% 198 20658 0.06% 199 19724 0.06% 200 18922 0.06% 201 18487 0.06% 202 17623 0.05% 203 17153 0.05% 204 16372 0.05% 205 15914 0.05% 206 15081 0.05% 207 14619 0.04% 208 13851 0.04% 209 13203 0.04% 210 12422 0.04% 211 11971 0.04% 212 11341 0.03% 213 11086 0.03% 214 10558 0.03% 215 9943 0.03% 216 9311 0.03% 217 8884 0.03% 218 8295 0.03% 219 7719 0.02% 220 7247 0.02% 221 7059 0.02% 222 6672 0.02% 223 6395 0.02% 224 6003 0.02% 225 5760 0.02% 226 5354 0.02% 227 4870 0.01% 228 4518 0.01% 229 4312 0.01% 230 4038 0.01% 231 3874 0.01% 232 3589 0.01% 233 3397 0.01% 234 3182 0.01% 235 2981 0.01% 236 2831 0.01% 237 2555 0.01% 238 2417 0.01% 239 2288 0.01% 240 2004 0.01% 241 1948 0.01% 242 1801 0.01% 243 1707 0.01% 244 1578 0.00% 245 1482 0.00% 246 1393 0.00% 247 1188 0.00% 248 1174 0.00% 249 1021 0.00% 250 1031 0.00% 251 915 0.00% 252 819 0.00% 253 790 0.00% 254 705 0.00% 255 653 0.00% 256 602 0.00% 257 553 0.00% 258 511 0.00% 259 461 0.00% 260 459 0.00% 261 367 0.00% 262 370 0.00% 263 339 0.00% 264 269 0.00% 265 271 0.00% 266 255 0.00% 267 197 0.00% 268 224 0.00% 269 161 0.00% 270 155 0.00% 271 139 0.00% 272 136 0.00% 273 123 0.00% 274 105 0.00% 275 100 0.00% 276 82 0.00% 277 82 0.00% 278 66 0.00% 279 64 0.00% 280 47 0.00% 281 37 0.00% 282 35 0.00% 283 22 0.00% 284 26 0.00% 285 27 0.00% 286 31 0.00% 287 16 0.00% 288 18 0.00% 289 10 0.00% 290 14 0.00% 291 9 0.00% 292 13 0.00% 293 9 0.00% 294 14 0.00% 295 9 0.00% 296 7 0.00% 297 7 0.00% 298 7 0.00% 299 5 0.00% 300 3 0.00% 301 4 0.00% 302 3 0.00% 303 3 0.00% 304 1 0.00% 305 1 0.00% 306 3 0.00% 307 3 0.00% 308 1 0.00% 309 5 0.00% 310 0 0.00% 311 3 0.00% 312 0 0.00% 313 2 0.00% 314 1 0.00% 315 1 0.00% 316 1 0.00% 317 1 0.00% 318 1 0.00% 319 1 0.00% 320 1 0.00% 321 0 0.00% 322 4 0.00% 323 0 0.00% 324 1 0.00% 325 1 0.00% 326 2 0.00% 327 1 0.00% 328 2 0.00% 329 1 0.00% 330 0 0.00% 331 0 0.00% 332 2 0.00% 333 1 0.00% 334 1 0.00% 335 0 0.00% 336 0 0.00% 337 1 0.00% 338 0 0.00% 339 2 0.00% 340 0 0.00% 341 0 0.00% 342 0 0.00% 343 0 0.00% 344 1 0.00% 345 2 0.00% 346 0 0.00% 347 0 0.00% 348 0 0.00% 349 0 0.00% 350 0 0.00% 351 2 0.00% 352 1 0.00% 353 0 0.00% 354 2 0.00% 355 1 0.00% 356 1 0.00% 357 1 0.00% 358 3 0.00% 359 1 0.00% 360 5 0.00% 361 1 0.00% 362 0 0.00% 363 0 0.00% 364 1 0.00% 33074475 reads passed initial QC criterion=sequence-density sequence-density=1.20 sequence-density-rank=1 fanout-score=2.06 fanout-score-rank=34 prefix-density=1.13 prefix-fanout=2.1 sequence=GCACCAGCTGCACCTGCGGCCACTGAAGCAGCAGCCTCTGATGCCTCTACCCGTACTACACTACTAGTAACCCCAGCTTAATTACGCTAACCCACGAGCACACGTGTCTGCTTGATGCGTGCGCACGTGGCGCGGCGTAAGCTATGACAATAAAAAGTGTGCTGTACCTGATGTGTCTGTGTGTCGATCTATGTCTGTCACGTACGTGGTCGTGCAAAAAACCCTGAAAGTTTAATTGGCTGGTTAATTTGTGCATAGGGAAATTAACAGTTGGAAAGGGCGATCGGTCTTGATCCCTCTGTGTTTCCCGTGTAACGGCTACTGATCCAGTGGTTAAGCTTCGGGTTGTATGTGAGTCCGTACGTGTTTGCATGGAATGAAATTTATCGTGTGGTCTTACTATCTA criterion=fanout-score sequence-density=0.02 sequence-density-rank=31 fanout-score=202.42 fanout-score-rank=1 prefix-density=2.33 prefix-fanout=1.9 sequence=TGAAGAAGGGAGGTGCTTTCACCGGTGAAGTCAGTGCTGAGATGCTTGCCAACCTTGGCATTCCCTGGGTCATTCTTGGACACTCTGAGAGGAGGAGCTTGTTGGGAGAATCAAGCGAGTTTGTTGGAGAGAAAGTTGCATACGCACTCGCTCAGGGCTTGAAGGTCATTGCATGTGTTGGCGAGACTCTCGAGCAGCGGGAAGCTGGGCAAACCATGGATGTTGTTGCTGAACAGACAAAAGCGATTGCTGCAAAGATCACAGATTGGACTAACGTAGTTGTTGCGTATGAGCCAGTGTGGGCCATTGGAACCGGTAAAGTTGCAACACCAGCTCAGGCACAGGAAGTGCATGCCAACCTCAGGGAGTGGCTCAAGA Started job on | Dec 10 00:49:12 Started mapping on | Dec 10 00:49:13 Finished on | Dec 10 00:50:09 Mapping speed, Million of reads per hour | 2126.22 Number of input reads | 33074475 Average input read length | 102 UNIQUE READS: Uniquely mapped reads number | 28186446 Uniquely mapped reads % | 85.22% Average mapped length | 100.33 Number of splices: Total | 7377541 Number of splices: Annotated (sjdb) | 6788684 Number of splices: GT/AG | 7109042 Number of splices: GC/AG | 91442 Number of splices: AT/AC | 5104 Number of splices: Non-canonical | 171953 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.24% Deletion average length | 1.13 Insertion rate per base | 0.23% Insertion average length | 1.18 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1304126 % of reads mapped to multiple loci | 3.94% Number of reads mapped to too many loci | 396014 % of reads mapped to too many loci | 1.20% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 8.61% % of reads unmapped: other | 1.03% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3583903 3583903 3583903 N_multimapping 1304126 1304126 1304126 N_noFeature 1088390 1294658 27389366 N_ambiguous 668857 86915 2579 UnstrandedReadsAssigned:26429199 PositiveStrandReadsAssigned:26804873 NegativeStrandReadsAssigned:794501 Dataset is classified positive stranded MeadianReadLen=97 20thPercentileLength=72 echo kmer=67 ERR1942996 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR1942996-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 33,074,475 reads, 27,909,926 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,190 rounds 52973 ERR1942996.ke.tsv 35125 ERR1942996.se.tsv 88098 total ==> ERR1942996.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 43.0188 2.33742 PNS24249 1928 1829 94.4592 2.6518 PNS24246 1044 945 43.0188 2.33742 PNS24248 1044 945 43.0188 2.33742 PNS24244 1471 1372 354.484 13.2664 PNS24243 293 194 0 0 KQK14069 1603 1504 11518 393.223 KQK14071 474 375 37.1113 5.08141 ==> ERR1942996.se.tsv <== BRADI_1g14170v3 12044 BRADI_1g53295v3 69 BRADI_1g59795v3 793 BRADI_1g07683v3 0 BRADI_1g00485v3 48 BRADI_1g20270v3 1184 BRADI_1g74790v3 493 BRADI_1g09890v3 63 BRADI_1g77505v3 886 BRADI_1g48960v3 0 ERR1942996 completed mapping pipeline successfully