Starting /dee2/code/volunteer_pipeline.sh ERR1942998 current disk space = 1523699863552 free memory = 1569141752 ERR1942998 SRAfilesize 346cbcfc19185b7ede7c4c2ce7edbe5e ERR1942998.sra ERR1942998.sra file validated ERR1942998 is single end ERR1942998 is conventional basespace ERR1942998 read1 length is 25-267 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1942998_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 25-267 %GC 62 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.8325 25.0 22.0 27.0 18.0 28.0 2 23.51125 25.0 22.0 27.0 15.0 28.0 3 23.259 25.0 21.0 27.0 14.0 28.0 4 23.6635 25.0 22.0 27.0 14.0 28.0 5 22.7655 24.0 20.0 27.0 14.0 28.0 6 23.45575 25.0 22.0 27.0 14.0 28.0 7 23.1875 24.0 21.0 27.0 14.0 28.0 8 23.566 25.0 22.0 27.0 14.0 28.0 9 23.506 25.0 21.0 27.0 15.0 28.0 10-14 23.899750000000004 24.8 21.8 27.0 16.6 28.6 15-19 24.31935 25.8 22.4 27.4 18.4 28.4 20-24 23.939299999999996 25.2 22.0 27.0 17.2 28.2 25-29 23.8831390887703 25.0 22.0 27.0 16.6 28.2 30-34 24.04964883733674 25.6 22.0 27.2 17.2 28.4 35-39 24.255164847612864 25.6 22.2 27.0 18.6 28.4 40-44 23.833832690585293 25.2 22.0 27.0 16.0 28.2 45-49 24.01515139544437 25.2 22.0 27.0 17.2 28.0 50-54 23.991112719907413 25.2 22.0 27.0 16.4 28.2 55-59 24.126980396300215 25.8 22.2 27.0 17.4 28.0 60-64 24.1263692325112 25.4 22.2 27.0 17.6 28.2 65-69 23.580627228667986 25.0 21.8 27.0 14.4 28.0 70-74 23.632168573746448 25.0 22.0 27.0 14.2 28.0 75-79 23.47167282530769 24.6 21.4 27.0 14.6 28.0 80-84 23.243862334205264 24.2 20.8 27.0 14.0 28.0 85-89 22.730835310841726 24.0 20.0 27.0 14.0 28.0 90-94 22.58170024540213 23.8 20.0 27.0 14.0 28.0 95-99 22.114521959666565 23.2 19.6 26.8 13.0 28.0 100-104 22.164612792722522 23.0 19.8 26.2 13.8 28.0 105-109 21.830754113418994 23.0 19.2 26.0 13.4 28.0 110-114 21.556820560395106 23.0 19.0 26.0 13.0 27.6 115-119 21.44945528213784 23.0 18.6 26.0 12.8 27.6 120-124 21.858687071140967 23.0 19.2 26.0 13.2 27.8 125-129 21.662421153262557 23.0 18.8 26.0 13.0 27.6 130-134 21.186721922069715 22.4 18.2 25.8 12.6 27.6 135-139 21.228974777861698 22.6 18.4 25.4 13.2 27.0 140-144 21.116596046912072 22.2 18.0 25.4 13.0 27.0 145-149 21.221699346369835 22.2 18.8 25.2 13.2 27.0 150-154 21.208685894849044 22.2 19.0 25.0 13.4 27.0 155-159 20.902877353434697 22.0 18.2 25.0 13.6 27.0 160-164 20.7154466178248 21.8 17.2 24.8 13.2 26.8 165-169 20.470138366990902 22.0 17.6 24.8 12.2 26.4 170-174 20.385605604200276 21.2 16.6 24.2 13.0 26.0 175-179 19.71474253884925 20.6 14.4 23.8 12.6 25.6 180-184 19.347005173660627 20.0 14.0 23.6 12.0 25.6 185-189 19.944424350928237 20.4 16.0 23.8 12.4 25.6 190-194 19.916370497712958 20.8 15.0 24.0 12.2 25.6 195-199 19.962734542960252 20.6 14.8 24.4 12.0 26.2 200-204 20.769653678986867 21.8 18.6 24.4 13.8 26.4 205-209 21.0732786695989 22.6 18.6 25.0 13.4 26.4 210-214 19.550403977147027 20.2 14.0 23.8 11.2 25.6 215-219 19.251112781741583 22.0 17.0 24.0 11.0 26.0 220-224 19.341410852713178 NaN NaN NaN NaN NaN 225-229 19.560915393322013 NaN NaN NaN NaN NaN 230-234 19.741486954935233 NaN NaN NaN NaN NaN 235-239 20.08836993119602 NaN NaN NaN NaN NaN 240-244 18.637705905205905 NaN NaN NaN NaN NaN 245-249 17.96222222222222 NaN NaN NaN NaN NaN 250-254 18.0 NaN NaN NaN NaN NaN 255-259 19.393333333333338 NaN NaN NaN NaN NaN 260-264 15.116666666666665 NaN NaN NaN NaN NaN 265-267 20.166666666666668 NaN NaN NaN NaN NaN >>END_MODULE >>Per sequence quality scores warn #Quality Count 15 4.0 16 9.0 17 29.0 18 98.0 19 212.0 20 361.0 21 422.0 22 476.0 23 554.0 24 659.0 25 586.0 26 458.0 27 131.0 28 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.325 37.45 10.05 26.174999999999997 2 36.15 30.95 7.1499999999999995 25.75 3 26.724999999999998 23.45 11.025 38.800000000000004 4 23.5 35.675000000000004 13.700000000000001 27.125 5 21.6 26.025 12.174999999999999 40.2 6 34.75 25.224999999999998 8.575000000000001 31.45 7 28.95 32.775 14.174999999999999 24.099999999999998 8 25.324999999999996 25.025 10.05 39.6 9 21.15 23.075000000000003 11.825 43.95 10-14 28.139999999999997 22.445 14.374999999999998 35.04 15-19 31.605 19.7 14.455000000000002 34.239999999999995 20-24 27.685 21.54 16.42 34.355000000000004 25-29 28.47058823529412 22.668335419274094 13.571964956195243 35.289111389236545 30-34 30.92560987852625 21.554060837265336 15.801626342736675 31.718702941471737 35-39 30.744713143782253 21.567337852878683 16.052817970251947 31.63513103308712 40-44 30.447381799694966 21.347229283172346 17.493645144890696 30.711743772241995 45-49 27.80224905425714 22.35062444939628 15.178525159351194 34.66860133699539 50-54 27.638384505337292 22.746781115879827 17.079344117970727 32.53549026081215 55-59 28.12023278151308 18.695971523814904 18.865472625572067 34.31832306909995 60-64 25.809457255998137 21.645702306079663 20.405310971348708 32.13952946657349 65-69 29.893778452200305 19.969650986342945 16.370257966616087 33.76631259484067 70-74 27.167520394612026 17.92196294188326 20.932144438120535 33.97837222538418 75-79 27.052374347797368 18.373951522356517 19.067432798362063 35.50624133148405 80-84 28.112703766160763 19.891793142214727 16.42776840921866 35.56773468240584 85-89 28.54663447026864 19.37820706308482 17.793540597645638 34.28161786900091 90-94 24.019015389573763 21.021674321166707 16.590121666263798 38.36918862299573 95-99 30.97850730026299 18.146368005803936 16.57749161149905 34.297633082434025 100-104 24.281984334203656 19.66258284796144 18.27676240208877 37.778670415746134 105-109 26.43265672311316 20.354379275541103 15.745205786699563 37.46775821464618 110-114 30.528476141611083 18.868650590046176 17.868137506413547 32.734735761929194 115-119 25.335449430096666 21.39662386380032 15.510027413071706 37.75789929303131 120-124 27.67603410167351 18.913798547521314 15.929902115566783 37.4802652352384 125-129 30.856748836407515 20.410274090674022 15.15255990346492 33.58041716945354 130-134 28.454832127620104 14.57985531441291 14.431459840474867 42.53385271749212 135-139 28.405921476078095 21.56189658871487 15.06114567689337 34.97103625831367 140-144 34.23193121078317 17.708575412502906 14.96630257959563 33.09319079711829 145-149 30.229007633587784 15.826972010178118 19.61832061068702 34.32569974554707 150-154 27.5639141933588 20.188069350573024 14.516602997355275 37.7314134587129 155-159 28.136624957727424 18.498478187352045 18.701386540412578 34.663510314507946 160-164 28.313253012048197 17.221828490432316 13.571934798015592 40.8929836995039 165-169 30.693845550764404 18.032144257154055 14.935319482555862 36.33869070952568 170-174 29.995569339831636 19.184758529020822 14.133805937084626 36.68586619406292 175-179 27.238605898123325 13.512064343163537 22.091152815013405 37.158176943699736 180-184 28.929663608562688 14.311926605504588 19.816513761467892 36.94189602446483 185-189 28.045515394912986 14.056224899598394 18.473895582329316 39.42436412315931 190-194 21.58920539730135 16.64167916041979 19.26536731634183 42.50374812593703 195-199 23.89455782312925 16.3265306122449 21.08843537414966 38.69047619047619 200-204 24.554826616682288 19.77507029053421 19.77507029053421 35.8950328022493 205-209 22.905027932960895 23.575418994413408 14.860335195530727 38.65921787709497 210-214 22.929936305732486 14.171974522292993 28.02547770700637 34.87261146496815 215-219 35.55045871559633 15.36697247706422 18.807339449541285 30.275229357798167 220-224 33.198380566801625 19.4331983805668 15.384615384615385 31.983805668016196 225-229 19.760479041916167 23.353293413173652 22.15568862275449 34.73053892215569 230-234 19.858156028368796 23.404255319148938 21.27659574468085 35.46099290780142 235-239 28.448275862068968 9.482758620689655 28.448275862068968 33.62068965517241 240-244 30.136986301369863 23.28767123287671 13.698630136986301 32.87671232876712 245-249 32.55813953488372 25.581395348837212 6.976744186046512 34.883720930232556 250-254 22.857142857142858 31.428571428571427 17.142857142857142 28.57142857142857 255-259 38.46153846153847 7.6923076923076925 11.538461538461538 42.30769230769231 260-264 40.0 20.0 20.0 20.0 265-267 50.0 33.33333333333333 0.0 16.666666666666664 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.5 27 1.0 28 1.0 29 1.5 30 2.0 31 1.5 32 1.5 33 3.0 34 7.333333333333333 35 9.666666666666666 36 7.5 37 9.5 38 14.666666666666666 39 12.666666666666666 40 20.833333333333336 41 30.0 42 18.0 43 15.5 44 42.333333333333336 45 54.33333333333333 46 61.833333333333336 47 52.66666666666667 48 29.833333333333332 49 26.5 50 25.5 51 28.0 52 26.0 53 25.0 54 39.16666666666668 55 43.000000000000014 56 26.5 57 29.0 58 44.5 59 66.0 60 145.5 61 246.5 62 328.5 63 470.3333333333337 64 531.1666666666672 65 451.0000000000001 66 381.16666666666674 67 282.33333333333337 68 223.33333333333331 69 188.0 70 115.0 71 82.5 72 52.833333333333336 73 39.5 74 61.5 75 119.5 76 164.5 77 100.5 78 27.5 79 11.0 80 2.5 81 3.5 82 3.5 83 2.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-154 0.0 155-159 0.0 160-164 0.0 165-169 0.0 170-174 0.0 175-179 0.0 180-184 0.0 185-189 0.0 190-194 0.0 195-199 0.0 200-204 0.0 205-209 0.0 210-214 0.0 215-219 0.0 220-224 0.0 225-229 0.0 230-234 0.0 235-239 0.0 240-244 0.0 245-249 0.0 250-254 0.0 255-259 0.0 260-264 0.0 265-267 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 25-29 9.0 30-34 31.0 35-39 16.0 40-44 52.0 45-49 127.0 50-54 206.0 55-59 72.0 60-64 146.0 65-69 130.0 70-74 130.0 75-79 178.0 80-84 153.0 85-89 195.0 90-94 187.0 95-99 319.0 100-104 147.0 105-109 259.0 110-114 196.0 115-119 138.0 120-124 122.0 125-129 60.0 130-134 129.0 135-139 118.0 140-144 61.0 145-149 97.0 150-154 122.0 155-159 18.0 160-164 47.0 165-169 61.0 170-174 74.0 175-179 59.0 180-184 25.0 185-189 36.0 190-194 33.0 195-199 25.0 200-204 23.0 205-209 53.0 210-214 42.0 215-219 44.0 220-224 22.0 225-229 8.0 230-234 3.0 235-239 9.0 240-244 8.0 245-249 3.0 250-254 1.0 255-259 2.0 260-264 2.0 265-268 2.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.125 #Duplication Level Percentage of deduplicated Percentage of total 1 72.86307053941908 21.95 2 8.049792531120332 4.8500000000000005 3 2.904564315352697 2.625 4 1.5767634854771784 1.9 5 2.0746887966804977 3.125 6 1.6597510373443984 3.0 7 0.9958506224066389 2.1 8 0.8298755186721992 2.0 9 0.9958506224066389 2.7 >10 7.468879668049793 41.6 >50 0.49792531120331945 11.55 >100 0.08298755186721991 2.6 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGA 104 2.6 No Hit CAGAAGAACTCGCTGGTGCAGTTCCACGTGCTGTCCACGGCGGTGCCCAT 97 2.4250000000000003 No Hit GACACGGATTCCACCGCGTCCCGGGCAGCCGGAGGTGGTGGGGGTGGCGG 93 2.325 No Hit CAGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAG 79 1.975 No Hit CGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTC 78 1.95 No Hit GTGTTCGCGCCCACGGACAACGCGTTCACGAGCCTGGCTTCCGGCACGCT 62 1.55 No Hit ACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGA 53 1.325 No Hit GGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCA 47 1.175 No Hit TCGACAACACGCTCTTCACCGGCGACCAGCTCGTGGTCTACCAGGTCAAC 46 1.15 No Hit GGCCAGCAGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAA 45 1.125 No Hit AGATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGAT 44 1.0999999999999999 No Hit AACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCG 41 1.0250000000000001 No Hit GCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCA 38 0.95 No Hit CGATTACACCACCAGAATGGAAGCCATGCAGAGATCATTTCTAGTGCTGC 37 0.9249999999999999 No Hit AACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGT 34 0.8500000000000001 No Hit ACCGGCAGCCGGAACGACCAAGACGACCACATAACAGGCGTGCTAGCGAA 34 0.8500000000000001 No Hit AGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAGC 32 0.8 No Hit CGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGC 32 0.8 No Hit ACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCAT 32 0.8 No Hit TGAAAAGAAGTGGTCATGGGGATTTTATTGCTCGAGATGCAGGTGTTCAG 32 0.8 No Hit AGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACACGCTC 30 0.75 No Hit CAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACATAACA 30 0.75 No Hit GGGTGGCGGCGTCGTCGCGGTGGCCATGGCATTGGCGTCCTGCGTCCTCT 30 0.75 No Hit CAGCTCCCCGGGGGAGTACCCGCTCAACGTCACCGCCACCGGCCAGCAGG 27 0.675 No Hit GACACGGATTCCACCGCGTCCCGGGCAGCCGGAGGTGGTGGGGTGGCGG 26 0.65 No Hit ACCACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATC 24 0.6 No Hit GGCATTGGCGTCCTGCGTCCTCTGCTGGGGGCTGTGACGTTGCC 23 0.575 No Hit ACCGGCAGCCGGAACGACCAAGACGACGCGGGCTCACGGTGTTCGCGCCC 23 0.575 No Hit CGGACAACGCGTTCACGAGCCTGGCTTCCGGCACGCTCAACTCGCTTTCG 22 0.5499999999999999 No Hit AGCAGCTCCCCGGGGGAGTACCCGCTCAACGTCACCGCCACCGGCCAGCA 20 0.5 No Hit TAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGGCTG 20 0.5 No Hit GGAAGCAAATCTAACCAAGGGAACCAATCTGATGAGTTGCAATTTGACAC 20 0.5 No Hit CATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTACACCACCAGAATGG 20 0.5 No Hit CCAGCAGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACA 20 0.5 No Hit GTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCACGGGCGTCGCC 19 0.475 No Hit ACACCGTGAGCAACCCGCTCAGGACGCAGGCCTGTTCGCGCCCACGGACA 19 0.475 No Hit TGTCCACGGCGGTGCCCATGTCGCAGTTCGACACCGTGAGCAACCCGCTC 19 0.475 No Hit AGTACCCGCTCAACGTCACCGCCACCGGCCAGCAGGTCAACATCTCCACG 18 0.44999999999999996 No Hit AGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAATG 18 0.44999999999999996 No Hit TCCGGCCAAGATCGACAACACGCTCTTCACCGGCGACCAGCTCGTGGTCT 18 0.44999999999999996 No Hit GGAACGACCAAGACGCCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAG 18 0.44999999999999996 No Hit GCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGC 18 0.44999999999999996 No Hit CCAAATCGACAACCAGCTCAACAGCTCCCAGACGGGCGGGCTCACGGTGT 17 0.42500000000000004 No Hit CAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCC 17 0.42500000000000004 No Hit ACGCAGGCCGGGAGCAGCTCCCCGGGGGAGTACCCGCTCAACGTCACCGC 17 0.42500000000000004 No Hit AGCCGGAGGTGGTGGGGGTGGCGGCGTCGTCGCGGTGGCCATGGCATTGGCGTCCTGCGTCC 17 0.42500000000000004 No Hit ACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTT 16 0.4 No Hit CACGGGGGTCGTCAATGCCACCGTCGACAACACGCTCTTCACCGGCGACCAGCTCGTGGTCTACCAGGTCAACC 16 0.4 No Hit AGCTCAACAGCTCCCAGACGGGCGGGCTCACGGTGTTCGCGCCCACGGAC 16 0.4 No Hit CCCAAATCGACAACCAGCTCAACAGCTCCAACATAACAGGCGTGCTAGCG 16 0.4 No Hit AATGGAGCCATGCAGAGATCATTTCTAGTGCTGCTCGTGATCTCAGCGGC 16 0.4 No Hit ACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGAC 16 0.4 No Hit AGCCTGGCTTCCGGCACGCTCAACTCGCTTTCGGACAGCCAGAAGAACTC 16 0.4 No Hit AGCTCCCAGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTT 15 0.375 No Hit TCCTCGCTGATAGCTAGAGTTTCACGATTACACCACCAGAATGGAAGCCA 14 0.35000000000000003 No Hit AACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGG 14 0.35000000000000003 No Hit GCGTTCACGAGCCTGGCTTCCGGCACGCTCAACTCGCTTTCGGACAGCCA 14 0.35000000000000003 No Hit CGCCCACGGACAACGCGTTCACGAGCCTGGCTTCCGGCACGCTCAACTCG 13 0.325 No Hit GACACGGATTCCACCGCGTCCCGGGCAGCCGGAGGTGGTGGGGGTGGGCGG 13 0.325 No Hit TCGCAGTTCGACACCGTGAGCAACCCGCTCAGGACGCAGGCCGGGAGCAG 13 0.325 No Hit TTCACGAGCCTGGCTTCCGGCACGCTCAACTCGCTTTCGGACAGCCAGAA 13 0.325 No Hit ACCGGCAGCCGGAACGACCAAGACGCCACATAACAGGCGTGCTAGCGAAA 13 0.325 No Hit CAGAAGAACTCGCTGGTGCAGTTCCACGTGCTGTCCACGCCACCGGCCAG 13 0.325 No Hit GCACCGGCAGCCGGAACGACCAAGACGCCAACATAACAGGCGTGCTAGCG 12 0.3 No Hit AGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAG 12 0.3 No Hit TGTCCACGGCGGTGCCCATGTCGCAGTTCGACACCGTGAGCAACCCGCTCAGGACGCAGGCCGGGAGC 12 0.3 No Hit TGTCCACGGCGGTGCCCATGTCGCAGTTCGACACCGTGAGCAACCCGCTCAGGACGCAGGCC 12 0.3 No Hit GGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCACGGGCGTCGCC 12 0.3 No Hit AATGCCACCGTCGACAACACGCTCTTCACCGGCG 12 0.3 No Hit GACCAACATAACAGGCGTCCACGGCGGTGCCCATGTCGCAGTTCGACTGT 12 0.3 No Hit ACACACACAAACCCTGCTGCACCCACCACCAATGCTACGTGCTCTGCTTG 12 0.3 No Hit ACTCCAATCTTGTTATAACTAAGCCACAGCTAAGCTCGCAAGCTCTCCAC 12 0.3 No Hit CCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACATAA 12 0.3 No Hit AGCGAAAGCCGGGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCACGG 11 0.27499999999999997 No Hit GCGTCGTCGCGGTGGCCATGGCATTGGCGTCCTGCGTCCTCTGCTGGGGG 11 0.27499999999999997 No Hit GCTGCTGCGTTCCACGGGCGTCGCCGCCCAAATCGACAACCAGCTCAACA 11 0.27499999999999997 No Hit TTCCACGGGCGTCGCCGCCCAAATCGACAACCAGCTCAACAGCTCCCAGA 11 0.27499999999999997 No Hit TGAGCAACCCGCTCAGGACGCAGGCCGGGAGCAGCTCCCCGGGGGAGTAC 11 0.27499999999999997 No Hit GTTCATCCGGCTGCTGCGTTCCACGGGCGTCGCCGCCCAAATCGACAACC 11 0.27499999999999997 No Hit CGTCGTCGCGGTGGCCATGGCATTGGCGTCCTGCGTCCTCTGCTGGGGGC 11 0.27499999999999997 No Hit CATGTCGCAGTTCGACACCGTGAGCAACCCGCTCAGGACGCAGGCCGGGA 11 0.27499999999999997 No Hit AGATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTACACCACCAG 11 0.27499999999999997 No Hit ACCGTAATTAATCCAACTTCCTCTGATTGAGTCGAATAAATTCAGATGGA 11 0.27499999999999997 No Hit AGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACACACGCTCT 11 0.27499999999999997 No Hit AAATCGACAACCAGCTCAACAGCTCCCAGACGGGCGGGCTCACGGTGTTC 11 0.27499999999999997 No Hit GGCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAG 11 0.27499999999999997 No Hit AGAAAAAAGGAGCGTGAGAGCCAAATGAATCGAAAGATTCATGTTTGGTT 10 0.25 No Hit ACAGCCAGAAGAACTCGCTGGTGCAGTTCCACGTGCTGTCCACGGCGGTG 10 0.25 No Hit CGACCACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCA 10 0.25 No Hit CACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCG 10 0.25 No Hit AGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACACGCTCTTCACCGGCGACCAGCTCG 10 0.25 No Hit GACACGGATTCCACCGCGTCCCGGGCAGCCGGAGGTGGTGGGGGTGGCGGCG 10 0.25 No Hit ACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGG 10 0.25 No Hit AGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAG 10 0.25 No Hit TCCTCGCTGATAGCTAGAGTTTCACGATTACACCACCAGAATGGAGCCAT 10 0.25 No Hit AACGACTCCAGCCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAG 10 0.25 No Hit GGACAACACGCTCTTCACCGGCGACCAGCTCGTGGTCTACCAGGTCAACC 10 0.25 No Hit CCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGG 9 0.22499999999999998 No Hit GTGGGGGGTGGCGGCGTCGTCGCGGTGGCCATGGCATTGGCGTCCTGCGT 9 0.22499999999999998 No Hit AACAGCTCCCAGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGC 9 0.22499999999999998 No Hit TGGCGGCGTCGTCGCGGTGGCCATGGCATTGGCGTCCTGCGTCCTCTGCT 9 0.22499999999999998 No Hit CGGGCAGTTCAACACGTTCATCCGGCTGCTGCACGGGGGTCGTCAATGCC 9 0.22499999999999998 No Hit AGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACA 9 0.22499999999999998 No Hit TTTCAGCTGAGGAGGGCAATGTACTAGTACTAGGTCAATATTTGAAGGAA 9 0.22499999999999998 No Hit ATCCCATGCAGCAGTCATTCCGAGCTATCATCCTACTACCTCTTCATCGG 9 0.22499999999999998 No Hit TCACCGGCGACCAGCTCGTGGTCTACCAGGTCAACCAGGTGCTGCTGG 9 0.22499999999999998 No Hit ATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGGCT 9 0.22499999999999998 No Hit ATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTACACCACCAG 9 0.22499999999999998 No Hit GTGGGGGTGGCGGCGTCGTCGCGGTGGCCATGGCATTGGCGTCCTGCGTC 9 0.22499999999999998 No Hit GCAGGGCCCAACGGCGGCGCCAACGACTCCAGCCCCAGCGGCTTCCGGCACGCTCAACTCGCTTTCGGACAGCC 8 0.2 No Hit AACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACACGCTCTTCAC 8 0.2 No Hit AGTGCTGCTCGTGATCTCAGCGGCGATCACGGCGTCGGCGCAGGGCCCAACGGCGGCGCCAACGACTCCAG 8 0.2 No Hit TCGACAACACGCTCTTCACCGGCGACCAGCTCGTGGTCTACCAGTCAACC 8 0.2 No Hit CAAGCTCTCCACCAGATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTACACCACC 8 0.2 No Hit AGAGATCATTTCTAGTGCTGCTCGTGATCTCAGCGGCGATCACGGCGGCC 8 0.2 No Hit GACACGGATTCCACCGCGTCTCCGGCCTCGCCGGCGCCACCCTGGCCCG 8 0.2 No Hit GTTCCACGGGCGTCGCCGCCCCAAATCGACAACCAGCTCAACAGCTCCCA 8 0.2 No Hit ACCAGATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCAC 8 0.2 No Hit GAGCAGTCTTGTCTGTATCACTGTTACCAGATCGGATATTACGGCAGTCT 8 0.2 No Hit AGCAGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACACG 7 0.17500000000000002 No Hit AATGGAAGCCATGCAGAGATCATTTCTAGTGCTGCTCGTGATCTCAGCGG 7 0.17500000000000002 No Hit GAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAG 7 0.17500000000000002 No Hit TCGTCGCGGTGGCCATGGCATTGGCGTCCCGCCTGAAGAATAAGCCATAC 7 0.17500000000000002 No Hit ACTCCATCTTGTTATAACTAAGCCACAGCTAAGCTCGCAAGCTCTCCACC 7 0.17500000000000002 No Hit GGCACCGGCAGCCGGAACGACCAAGACGCCAACATAACAGGCGTGCTAGC 7 0.17500000000000002 No Hit GGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAGCCTGGCTTC 7 0.17500000000000002 No Hit ACCGGCCAGCAGGTCAACATCTCCACGGCCCGGGGGAGTACCCGCTCAACGTCACCGCC 7 0.17500000000000002 No Hit TCAGGACGCAGGCCGGGAGCAGCTCCCCGGGGGAGTACCCGCTCAACGTC 7 0.17500000000000002 No Hit CGGAACGACCAAGACGACCACATAACAGGCGTGCTAGCGAAAGCCGGGCA 7 0.17500000000000002 No Hit AGCCTGGCTTCCGGCACGCTCAACTCGCTTTCGGACAGCCAGAAGAACTCGCTGGTGCAGTTCCACGTGC 7 0.17500000000000002 No Hit CGATTACACCACCAGAATGGAGCCATGCAGAGATCATTTCTAGTGCTGCT 7 0.17500000000000002 No Hit GGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCACGGGCGTCGCCGCC 6 0.15 No Hit GGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAGCCTGGC 6 0.15 No Hit AGTGCTGCTCGTGATCTCAGCGGAGGATTAAGGGCCCGGCGTTGGGGACTATCGCGGAGGACG 6 0.15 No Hit AGTACCGCTCAACGTCACCGCCACCGGCCAGCAGGTCAACATCTCCACGG 6 0.15 No Hit ATCCCCATGCAGCAGTCATTCCGAGCTATCATCCTACTACCTCTTCATCG 6 0.15 No Hit CCGGCAGAAGAACTCGCTGGTGCAGTTCCACGTGCTGTCCACGGCGGTGC 6 0.15 No Hit CTCTCCCTTGTCACGCTCCTTTTTTATTACTTCCAAATTACTACAAACAA 6 0.15 No Hit CCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACATA 6 0.15 No Hit AGTGCTGCTCGTGATCTCAGCGGCGATCACGGCGTCGGCGCAGGGCCCAACGGCGGCGCCAACGACTCC 6 0.15 No Hit GAAGAAGGCGGACGCGCCGGCCCCCGCGCCGCTGGGTCCGGCCAAGAAGACGCCGG 6 0.15 No Hit GGTGCCCATGTCGCAGTTCGACACCGTGAGCAACCCGCTCAGGAC 6 0.15 No Hit AAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACAC 6 0.15 No Hit CGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTC 6 0.15 No Hit TCCACGGGCGTCGCCGCCCCAAATCGACAACCAGCTCAACAGCTCCCAGACGGGCGGGCTCACGGG 6 0.15 No Hit ACAACACGCTCTTCACCGGCGACCAGCTCGTGGTCTACCAGGTCAACC 6 0.15 No Hit CACCGGCAGCCGGAACGACCAAGACGCCAACATAACAGGCGTGCTAGCGA 6 0.15 No Hit CAGACGGGCGGGCGTCACGGTGTTCGCGCCCACGGACAACGCGTTCACGA 6 0.15 No Hit CGCCAACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACC 6 0.15 No Hit GACAACGCGTTCACGAGCCTGGCTTCCGGCACGCTCAACTCGCTTTCGGA 6 0.15 No Hit CGACCAAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTT 6 0.15 No Hit CGCAGGCCGGGAGCAGCTCCCCGGGGGAGTACCCGCTCAACGTCACCGCC 5 0.125 No Hit GCAGTTCGACACCGTGAGCAACCCGCTCAGGACGCAGGCCGGGAGCAGCTCCCCGGGGG 5 0.125 No Hit CAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAAC 5 0.125 No Hit AGGTCAACCAGGTGCTGCTGCCCATGGCCATCGCCGGGAAGAAGGC 5 0.125 No Hit ACCGGCAGCCGGAACGACCAAGACGACCAAACATAACAGGCGTGCTAGCG 5 0.125 No Hit ACCGGCAGCCGGAACGACCAAGACGCCAACATAACAGGCGTGCTAGCGAA 5 0.125 No Hit ACACGTTCATCCGGCTGCTGCGTTCCACGGGCGTCGCCGCCCAAATCGAG 5 0.125 No Hit CAGCTCCCCCGGGGGAGTACCCGCTCAACGTCACCGCCACCGGCCAGCAG 5 0.125 No Hit AGTGCTGCTCGTGATCTCAGCGGCGATCACGCGTCGGCGCAGGGCCCAACGG 5 0.125 No Hit AGCCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAG 5 0.125 No Hit AAGACGCCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACG 5 0.125 No Hit GCCAGCAGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACACGCTCTTCACCGGCGACCAGCTCG 5 0.125 No Hit TCCACGGCGTCGCCGCCCCAAATCGACAACCAGCTCAACAGCTCCCAGACGGGCGGGCTCACGGG 5 0.125 No Hit CGGTGGCCATGGCATTGGCGTCCTGCGTCCTCTGCTGGGGGCTGTGACGT 5 0.125 No Hit CACCGGCAGCCGGAACGACCAAGACGACCACATAACAGGCGTGCTAGCGA 5 0.125 No Hit GGGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCACGGGCGTCGCCGC 5 0.125 No Hit GAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGGCTGC 5 0.125 No Hit AGCAGCCGGAACGACCAAGACGCCAACATAACAGGCGTGCTAGCGAATGG 5 0.125 No Hit TCCACGTGCTGTCCACGGCGGTGCCCATGTCGCAGTTCGACACCGTGAGC 5 0.125 No Hit ACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGAC 5 0.125 No Hit GGAGCAGCTCCCCGGGGGAGTACCCGCTCAACGTCACCGCCACCGGCCAGCAGGTCAACATCTCCACGGGGGT 5 0.125 No Hit GGGGTGGCGGCGTCGTCGCGGTGGCCATGGCATTGGCGTCCTGCGTCCTC 5 0.125 No Hit AGAGATCATTCTAGTGCTGCTCGTGATCTCAGCGGCGATCACGGCGGCCA 5 0.125 No Hit CCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGC 5 0.125 No Hit GGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCACGGCGTCGCCGCCC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-14 0.0 0.0 0.0 0.0 0.0 15-19 0.0 0.0 0.0 0.0 0.0 20-24 0.0 0.0 0.0 0.0 0.0 25-29 0.0 0.0 0.0 0.0 0.0 30-34 0.0 0.0 0.0 0.0 0.0 35-39 0.0 0.0 0.0 0.0 0.0 40-44 0.0 0.0 0.0 0.0 0.0 45-49 0.0 0.0 0.0 0.0 0.0 50-54 0.0 0.0 0.0 0.0 0.0 55-59 0.0 0.0 0.0 0.0 0.0 60-64 0.0 0.0 0.0 0.0 0.0 65-69 0.0 0.0 0.0 0.0 0.0 70-74 0.0 0.0 0.0 0.0 0.0 75-79 0.0 0.0 0.0 0.0 0.0 80-84 0.0 0.0 0.0 0.0 0.0 85-89 0.0 0.0 0.0 0.0 0.0 90-94 0.0 0.0 0.0 0.0 0.0 95-99 0.0 0.0 0.0 0.0 0.0 100-104 0.0 0.0 0.0 0.0 0.0 105-109 0.0 0.0 0.0 0.0 0.0 110-114 0.0 0.0 0.0 0.0 0.0 115-119 0.0 0.0 0.0 0.0 0.0 120-124 0.0 0.0 0.0 0.0 0.0 125-129 0.0 0.0 0.0 0.0 0.0 130-134 0.0 0.0 0.0 0.0 0.0 135-139 0.0 0.0 0.0 0.0 0.0 140-144 0.0 0.0 0.0 0.0 0.0 145-149 0.0 0.0 0.0 0.0 0.0 150-154 0.0 0.0 0.0 0.0 0.0 155-159 0.0 0.0 0.0 0.0 0.0 160-164 0.0 0.0 0.0 0.0 0.0 165-169 0.0 0.0 0.0 0.0 0.0 170-174 0.0 0.0 0.0 0.0 0.0 175-179 0.0 0.0 0.0 0.0 0.0 180-184 0.0 0.0 0.0 0.0 0.0 185-189 0.0 0.0 0.0 0.0 0.0 190-194 0.0 0.0 0.0 0.0 0.0 195-199 0.0 0.0 0.0 0.0 0.0 200-204 0.0 0.0 0.0 0.0 0.0 205-209 0.0 0.0 0.0 0.0 0.0 210-214 0.0 0.0 0.0 0.0 0.0 215-219 0.0 0.0 0.0 0.0 0.0 220-224 0.0 0.0 0.0 0.0 0.0 225-229 0.0 0.0 0.0 0.0 0.0 230-234 0.0 0.0 0.0 0.0 0.0 235-239 0.0 0.0 0.0 0.0 0.0 240-244 0.0 0.0 0.0 0.0 0.0 245-249 0.0 0.0 0.0 0.0 0.0 250-254 0.0 0.0 0.0 0.0 0.0 255 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTCCT 5 0.005625378 1117.0 160-164 CTTTCGG 50 0.0 781.9 210 ACACGGA 20 1.3366451E-5 97.7375 2 AACGACT 20 1.3366451E-5 97.7375 1 CTCCAGC 20 1.3366451E-5 97.7375 6 GGATTCC 20 1.3366451E-5 97.7375 6 CGGATTC 20 1.3366451E-5 97.7375 5 ACTCCAG 20 1.3366451E-5 97.7375 5 TCCAGCC 20 1.3366451E-5 97.7375 7 GACACGG 20 1.3366451E-5 97.7375 1 TTCCACC 20 1.3366451E-5 97.7375 9 GATTCCA 20 1.3366451E-5 97.7375 7 ACGACTC 20 1.3366451E-5 97.7375 2 ATTCCAC 20 1.3366451E-5 97.7375 8 GACTCCA 20 1.3366451E-5 97.7375 4 ACGGATT 20 1.3366451E-5 97.7375 4 CGACTCC 20 1.3366451E-5 97.7375 3 CACGGAT 20 1.3366451E-5 97.7375 3 CAGCCCC 25 4.046658E-5 78.19 9 CCAGCCC 25 4.046658E-5 78.19 8 >>END_MODULE Read 1345867 spots for ERR1942998.sra Written 1345867 spots for ERR1942998.sra Read 1345867 spots for ERR1942998.sra Written 1345867 spots for ERR1942998.sra Read 1345867 spots for ERR1942998.sra Written 1345867 spots for ERR1942998.sra Read 1345867 spots for ERR1942998.sra Written 1345867 spots for ERR1942998.sra Read 1345867 spots for ERR1942998.sra Written 1345867 spots for ERR1942998.sra Read 1345867 spots for ERR1942998.sra Written 1345867 spots for ERR1942998.sra Read 1345867 spots for ERR1942998.sra Written 1345867 spots for ERR1942998.sra Read 1345867 spots for ERR1942998.sra Written 1345867 spots for ERR1942998.sra Read 1345867 spots for ERR1942998.sra Written 1345867 spots for ERR1942998.sra Read 1345875 spots for ERR1942998.sra Written 1345875 spots for ERR1942998.sra Read 1345867 spots for ERR1942998.sra Written 1345867 spots for ERR1942998.sra Read 1345867 spots for ERR1942998.sra Written 1345867 spots for ERR1942998.sra Read 1345867 spots for ERR1942998.sra Written 1345867 spots for ERR1942998.sra Read 1345867 spots for ERR1942998.sra Written 1345867 spots for ERR1942998.sra Read 1345867 spots for ERR1942998.sra Written 1345867 spots for ERR1942998.sra Read 1345867 spots for ERR1942998.sra Written 1345867 spots for ERR1942998.sra Read 1345867 spots for ERR1942998.sra Written 1345867 spots for ERR1942998.sra Read 1345867 spots for ERR1942998.sra Written 1345867 spots for ERR1942998.sra Read 1345867 spots for ERR1942998.sra Written 1345867 spots for ERR1942998.sra Read 1345867 spots for ERR1942998.sra Written 1345867 spots for ERR1942998.sra SRR ids: ['ERR1942998.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_8bdybzr_ ERR1942998.sra spots: 26917348 blocks: [[1, 1345867], [1345868, 2691734], [2691735, 4037601], [4037602, 5383468], [5383469, 6729335], [6729336, 8075202], [8075203, 9421069], [9421070, 10766936], [10766937, 12112803], [12112804, 13458670], [13458671, 14804537], [14804538, 16150404], [16150405, 17496271], [17496272, 18842138], [18842139, 20188005], [20188006, 21533872], [21533873, 22879739], [22879740, 24225606], [24225607, 25571473], [25571474, 26917348]] ERR1942998 file size 7327128 ERR1942998 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR1942998 ERR1942998_1.fastq Input file: ERR1942998_1.fastq trimmed: ERR1942998-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Tue Dec 10 00:56:25 2024 >> started Tue Dec 10 00:56:40 2024 >> done (15.724s) 26917348 reads processed; of these: 394 ( 0.00%) short reads filtered out after trimming by size control 121 ( 0.00%) empty reads filtered out after trimming by size control 26916833 (100.00%) reads available; of these: 1470441 ( 5.46%) trimmed reads available after processing 25446392 (94.54%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 397 0.00% 19 642 0.00% 20 1127 0.00% 21 1715 0.01% 22 2271 0.01% 23 3122 0.01% 24 7719 0.03% 25 38334 0.14% 26 38468 0.14% 27 39789 0.15% 28 40141 0.15% 29 41132 0.15% 30 42202 0.16% 31 43332 0.16% 32 43426 0.16% 33 44507 0.17% 34 44425 0.17% 35 46113 0.17% 36 46648 0.17% 37 47330 0.18% 38 48768 0.18% 39 49626 0.18% 40 50219 0.19% 41 52208 0.19% 42 53707 0.20% 43 54563 0.20% 44 56175 0.21% 45 58458 0.22% 46 60535 0.22% 47 61399 0.23% 48 64413 0.24% 49 65512 0.24% 50 67373 0.25% 51 70576 0.26% 52 72537 0.27% 53 75272 0.28% 54 77972 0.29% 55 79601 0.30% 56 83494 0.31% 57 85862 0.32% 58 87190 0.32% 59 92720 0.34% 60 97394 0.36% 61 98354 0.37% 62 102737 0.38% 63 106583 0.40% 64 106868 0.40% 65 110308 0.41% 66 112910 0.42% 67 117901 0.44% 68 122087 0.45% 69 130431 0.48% 70 132949 0.49% 71 132863 0.49% 72 138467 0.51% 73 139860 0.52% 74 147755 0.55% 75 151101 0.56% 76 153410 0.57% 77 160064 0.59% 78 165336 0.61% 79 165149 0.61% 80 168973 0.63% 81 174134 0.65% 82 182505 0.68% 83 185895 0.69% 84 194296 0.72% 85 196295 0.73% 86 202231 0.75% 87 204908 0.76% 88 206481 0.77% 89 207849 0.77% 90 219668 0.82% 91 221213 0.82% 92 222382 0.83% 93 230350 0.86% 94 235690 0.88% 95 246014 0.91% 96 251297 0.93% 97 248408 0.92% 98 265328 0.99% 99 252430 0.94% 100 257165 0.96% 101 259411 0.96% 102 261871 0.97% 103 260741 0.97% 104 263210 0.98% 105 266907 0.99% 106 265736 0.99% 107 270800 1.01% 108 276734 1.03% 109 284462 1.06% 110 281675 1.05% 111 282780 1.05% 112 283578 1.05% 113 271936 1.01% 114 277864 1.03% 115 281192 1.04% 116 274689 1.02% 117 279360 1.04% 118 284639 1.06% 119 274417 1.02% 120 274169 1.02% 121 268196 1.00% 122 264997 0.98% 123 265365 0.99% 124 260870 0.97% 125 258059 0.96% 126 262557 0.98% 127 256392 0.95% 128 254547 0.95% 129 254693 0.95% 130 249692 0.93% 131 242879 0.90% 132 239645 0.89% 133 234158 0.87% 134 237457 0.88% 135 233348 0.87% 136 229489 0.85% 137 224180 0.83% 138 231025 0.86% 139 219959 0.82% 140 219830 0.82% 141 217565 0.81% 142 204679 0.76% 143 199651 0.74% 144 201173 0.75% 145 195789 0.73% 146 193919 0.72% 147 189855 0.71% 148 186022 0.69% 149 179028 0.67% 150 174494 0.65% 151 171042 0.64% 152 167558 0.62% 153 166494 0.62% 154 163243 0.61% 155 157063 0.58% 156 150109 0.56% 157 148088 0.55% 158 143268 0.53% 159 143224 0.53% 160 137224 0.51% 161 135961 0.51% 162 140722 0.52% 163 139110 0.52% 164 130869 0.49% 165 122189 0.45% 166 116678 0.43% 167 115006 0.43% 168 113978 0.42% 169 108321 0.40% 170 105718 0.39% 171 103022 0.38% 172 100546 0.37% 173 95663 0.36% 174 91400 0.34% 175 87539 0.33% 176 84913 0.32% 177 84643 0.31% 178 81629 0.30% 179 78977 0.29% 180 75449 0.28% 181 72549 0.27% 182 70058 0.26% 183 67353 0.25% 184 64720 0.24% 185 62018 0.23% 186 61158 0.23% 187 59522 0.22% 188 56708 0.21% 189 53586 0.20% 190 51310 0.19% 191 49324 0.18% 192 48102 0.18% 193 45825 0.17% 194 43633 0.16% 195 42209 0.16% 196 40712 0.15% 197 39086 0.15% 198 37623 0.14% 199 35930 0.13% 200 34614 0.13% 201 33704 0.13% 202 32263 0.12% 203 31564 0.12% 204 30705 0.11% 205 30020 0.11% 206 28381 0.11% 207 27576 0.10% 208 25904 0.10% 209 24543 0.09% 210 23172 0.09% 211 22218 0.08% 212 21378 0.08% 213 20996 0.08% 214 19866 0.07% 215 18721 0.07% 216 17957 0.07% 217 16914 0.06% 218 15914 0.06% 219 15261 0.06% 220 14225 0.05% 221 13737 0.05% 222 12798 0.05% 223 12192 0.05% 224 11603 0.04% 225 11052 0.04% 226 10472 0.04% 227 9702 0.04% 228 9147 0.03% 229 8750 0.03% 230 8202 0.03% 231 7768 0.03% 232 7270 0.03% 233 6892 0.03% 234 6350 0.02% 235 5847 0.02% 236 5554 0.02% 237 5258 0.02% 238 4910 0.02% 239 4477 0.02% 240 4188 0.02% 241 3757 0.01% 242 3792 0.01% 243 3496 0.01% 244 3184 0.01% 245 2874 0.01% 246 2710 0.01% 247 2577 0.01% 248 2366 0.01% 249 2179 0.01% 250 1984 0.01% 251 1832 0.01% 252 1643 0.01% 253 1599 0.01% 254 1467 0.01% 255 1367 0.01% 256 1275 0.00% 257 1151 0.00% 258 1013 0.00% 259 967 0.00% 260 880 0.00% 261 799 0.00% 262 740 0.00% 263 705 0.00% 264 659 0.00% 265 574 0.00% 266 526 0.00% 267 462 0.00% 268 414 0.00% 269 394 0.00% 270 373 0.00% 271 322 0.00% 272 264 0.00% 273 247 0.00% 274 190 0.00% 275 205 0.00% 276 177 0.00% 277 150 0.00% 278 146 0.00% 279 138 0.00% 280 135 0.00% 281 101 0.00% 282 95 0.00% 283 70 0.00% 284 64 0.00% 285 73 0.00% 286 62 0.00% 287 50 0.00% 288 52 0.00% 289 26 0.00% 290 34 0.00% 291 29 0.00% 292 23 0.00% 293 23 0.00% 294 20 0.00% 295 15 0.00% 296 20 0.00% 297 14 0.00% 298 17 0.00% 299 11 0.00% 300 16 0.00% 301 13 0.00% 302 20 0.00% 303 10 0.00% 304 5 0.00% 305 10 0.00% 306 9 0.00% 307 7 0.00% 308 7 0.00% 309 10 0.00% 310 6 0.00% 311 7 0.00% 312 8 0.00% 313 3 0.00% 314 6 0.00% 315 11 0.00% 316 2 0.00% 317 3 0.00% 318 1 0.00% 319 4 0.00% 320 5 0.00% 321 4 0.00% 322 5 0.00% 323 6 0.00% 324 2 0.00% 325 7 0.00% 326 3 0.00% 327 5 0.00% 328 4 0.00% 329 4 0.00% 330 4 0.00% 331 3 0.00% 332 3 0.00% 333 4 0.00% 334 1 0.00% 335 3 0.00% 336 4 0.00% 337 6 0.00% 338 2 0.00% 339 3 0.00% 340 7 0.00% 341 5 0.00% 342 3 0.00% 343 4 0.00% 344 3 0.00% 345 9 0.00% 346 4 0.00% 347 10 0.00% 348 11 0.00% 349 6 0.00% 350 7 0.00% 351 7 0.00% 352 9 0.00% 353 19 0.00% 354 9 0.00% 355 14 0.00% 356 17 0.00% 357 8 0.00% 358 10 0.00% 359 7 0.00% 360 5 0.00% 361 4 0.00% 362 3 0.00% 363 2 0.00% 364 1 0.00% 365 1 0.00% 26916833 reads passed initial QC criterion=sequence-density sequence-density=2.45 sequence-density-rank=1 fanout-score=2.22 fanout-score-rank=36 prefix-density=2.61 prefix-fanout=2.1 sequence=AAAAAACCCTGAAAGTTTAATTGGCTGGTTAATTTGTGCATAGGGAAATTAACAGTTGGAAAGGGCGATCGGTCTTGATCCCTCTGTGTTTCCCGTGTAACGGCTACTGATCCAGTGGTTAAGCTTCGGGTTGTATGTGAGTCCGTACGTGTTTGCATGGAATGAAATTTATCGTGTGGTCTTACTATCTA criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=314.64 fanout-score-rank=1 prefix-density=2.12 prefix-fanout=1.1 sequence=TTAATTGGCCGGGTCGTGCCTCCGGCATCGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCATCGCTCTGGATACATTAGCATGGGATAACATCATAGGATTCCGGTCCTATTGTGTTGGCCTCGGGATCGGAGTAATGATTAATAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTGCTTATAGGACTCCGCCGGCACCTTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGCGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTACCAGGTCCAGACATAGCA Potential 3prime adapter identified. Now checking if in reference sequence Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192. 1 reads; of these: 1 (100.00%) were unpaired; of these: 0 (0.00%) aligned 0 times 1 (100.00%) aligned exactly 1 time 0 (0.00%) aligned >1 times 100.00% overall alignment rate Potential adapter found in reference sequence. Continuing without clipping. Started job on | Dec 10 00:56:59 Started mapping on | Dec 10 00:57:00 Finished on | Dec 10 00:57:57 Mapping speed, Million of reads per hour | 1700.01 Number of input reads | 26916833 Average input read length | 117 UNIQUE READS: Uniquely mapped reads number | 22644882 Uniquely mapped reads % | 84.13% Average mapped length | 115.37 Number of splices: Total | 7241877 Number of splices: Annotated (sjdb) | 6570946 Number of splices: GT/AG | 6876433 Number of splices: GC/AG | 90924 Number of splices: AT/AC | 5648 Number of splices: Non-canonical | 268872 Mismatch rate per base, % | 0.43% Deletion rate per base | 0.28% Deletion average length | 1.17 Insertion rate per base | 0.27% Insertion average length | 1.18 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 916902 % of reads mapped to multiple loci | 3.41% Number of reads mapped to too many loci | 266706 % of reads mapped to too many loci | 0.99% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 10.84% % of reads unmapped: other | 0.63% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3355049 3355049 3355049 N_multimapping 916902 916902 916902 N_noFeature 779266 949668 22003645 N_ambiguous 540107 78530 2445 UnstrandedReadsAssigned:21325509 PositiveStrandReadsAssigned:21616684 NegativeStrandReadsAssigned:638792 Dataset is classified positive stranded MeadianReadLen=116 20thPercentileLength=84 echo kmer=79 ERR1942998 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR1942998-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 26,916,833 reads, 22,739,193 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,217 rounds 52973 ERR1942998.ke.tsv 35125 ERR1942998.se.tsv 88098 total ==> ERR1942998.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 1.36575 0.102216 PNS24247 1044 945 41.6826 2.7631 PNS24249 1928 1829 28.0154 0.959526 PNS24246 1044 945 41.6826 2.7631 PNS24248 1044 945 41.6826 2.7631 PNS24244 1471 1372 290.571 13.267 PNS24243 293 194 0 0 KQK14069 1603 1504 8136.18 338.88 KQK14071 474 375 134.775 22.5139 ==> ERR1942998.se.tsv <== BRADI_1g14170v3 8093 BRADI_1g53295v3 103 BRADI_1g59795v3 368 BRADI_1g07683v3 0 BRADI_1g00485v3 46 BRADI_1g20270v3 1078 BRADI_1g74790v3 376 BRADI_1g09890v3 30 BRADI_1g77505v3 522 BRADI_1g48960v3 1 ERR1942998 completed mapping pipeline successfully