Starting /dee2/code/volunteer_pipeline.sh ERR1943000 current disk space = 1523686535168 free memory = 1431184988 ERR1943000 SRAfilesize fd3c7624024ee99519adc8b4985d7b62 ERR1943000.sra ERR1943000.sra file validated ERR1943000 is single end ERR1943000 is conventional basespace ERR1943000 read1 length is 25-248 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1943000_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 25-248 %GC 58 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.6685 26.0 23.0 27.0 20.0 29.0 2 24.1615 26.0 22.0 27.0 17.0 29.0 3 24.12625 25.0 22.0 27.0 17.0 29.0 4 24.35775 26.0 23.0 28.0 17.0 29.0 5 24.09925 26.0 22.0 27.0 15.0 29.0 6 24.44025 26.0 23.0 28.0 17.0 29.0 7 23.65525 24.0 22.0 27.0 18.0 28.0 8 24.5245 26.0 23.0 27.0 19.0 29.0 9 23.65725 25.0 21.0 27.0 15.0 29.0 10-14 24.265 25.6 22.4 27.2 17.8 28.6 15-19 23.843 25.2 22.0 27.0 15.8 28.8 20-24 23.813200000000002 25.0 22.0 27.0 16.6 28.0 25-29 23.61849016909005 24.6 21.6 27.0 15.8 28.0 30-34 23.575831202873513 24.6 21.4 27.0 15.6 28.2 35-39 23.72888429653619 24.6 21.8 27.0 16.6 28.0 40-44 23.44557977776739 24.8 21.6 27.0 14.2 28.0 45-49 23.351936080959916 24.6 21.2 27.0 14.6 28.0 50-54 23.50631631095608 24.8 21.4 27.0 14.8 28.0 55-59 23.246598197725312 24.2 21.2 27.0 14.0 28.0 60-64 22.68976367548712 24.0 20.2 27.0 13.8 28.0 65-69 22.66964763544086 23.8 20.0 27.0 14.0 28.0 70-74 22.026791559994695 23.4 19.6 27.0 12.8 28.0 75-79 22.235910455156237 23.4 19.6 26.8 13.8 28.0 80-84 21.749590689113454 22.6 18.8 26.2 13.6 28.0 85-89 21.574334824936606 22.8 18.8 26.0 12.8 28.0 90-94 21.403232470587696 22.6 18.4 25.8 12.8 27.8 95-99 21.74309793871809 22.8 19.0 26.0 13.2 27.8 100-104 21.72412727393027 23.0 19.0 26.0 13.8 27.8 105-109 21.607735001249388 22.8 18.8 25.4 14.0 27.6 110-114 21.503557415599182 22.8 18.8 26.0 13.2 27.0 115-119 20.922497245785447 22.0 17.8 25.2 12.6 27.0 120-124 21.370756438918285 22.6 18.4 25.8 13.0 27.6 125-129 21.76024295175855 23.0 19.0 26.0 13.0 27.8 130-134 21.500701294057315 22.8 18.4 25.8 13.0 27.8 135-139 21.243135482780325 22.0 18.4 25.0 13.4 27.0 140-144 21.04163545046739 22.0 18.0 25.0 13.0 27.0 145-149 21.13780514380217 22.2 18.2 25.2 13.0 27.2 150-154 20.72914355556788 21.6 18.0 25.0 13.2 26.8 155-159 20.750438128950577 21.8 17.4 24.6 13.4 26.6 160-164 20.222173424420724 21.2 15.8 24.4 11.6 26.4 165-169 19.99206981502008 20.6 15.6 24.0 11.8 26.0 170-174 20.305520774761703 21.0 16.6 24.2 12.6 25.8 175-179 19.73226162981032 20.8 15.0 23.8 12.0 25.4 180-184 19.54121965905795 20.2 15.2 23.4 11.6 25.2 185-189 19.93361572271185 20.4 17.0 23.6 12.0 25.2 190-194 19.031754662394157 20.0 14.0 23.25 12.0 25.25 195-199 19.346838170526343 NaN NaN NaN NaN NaN 200-204 19.042804206276465 NaN NaN NaN NaN NaN 205-209 19.77286730076646 NaN NaN NaN NaN NaN 210-214 18.93474985584001 NaN NaN NaN NaN NaN 215-219 17.368711673939703 NaN NaN NaN NaN NaN 220-224 18.083737373737375 NaN NaN NaN NaN NaN 225-229 19.22556561085973 NaN NaN NaN NaN NaN 230-234 19.355454545454545 NaN NaN NaN NaN NaN 235-239 18.928174603174604 NaN NaN NaN NaN NaN 240-244 18.28333333333333 NaN NaN NaN NaN NaN 245-248 18.0 NaN NaN NaN NaN NaN >>END_MODULE >>Per sequence quality scores warn #Quality Count 15 1.0 16 15.0 17 61.0 18 147.0 19 295.0 20 448.0 21 538.0 22 559.0 23 572.0 24 516.0 25 487.0 26 309.0 27 52.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.225 33.650000000000006 32.15 14.975 2 47.275 31.724999999999998 6.575 14.424999999999999 3 22.35 16.5 10.674999999999999 50.475 4 39.75 19.875 18.725 21.65 5 20.0 28.125 27.1 24.775 6 26.025 15.8 11.4 46.775 7 29.549999999999997 16.075 18.0 36.375 8 21.575 11.375 24.025 43.025000000000006 9 25.525 18.025 7.775 48.675000000000004 10-14 30.4 14.02 19.48 36.1 15-19 42.66 17.435000000000002 10.92 28.985 20-24 30.685000000000002 16.79 19.505 33.019999999999996 25-29 31.65932305227318 18.085319447226116 18.125375525736033 32.12998197476467 30-34 27.691920463126102 20.573873647118045 13.959224767178455 37.7749811225774 35-39 31.072354705852444 22.47928001220318 15.411603193166219 31.036762088778158 40-44 30.364746319191504 23.269891756014978 16.2878982198738 30.077463704919715 45-49 22.325702393340272 20.894901144640997 20.34859521331946 36.430801248699275 50-54 27.907225882478976 20.943810595104186 18.490545824629066 32.658417697787776 55-59 21.305371516180255 26.868532004599466 18.425231342057714 33.40086513716257 60-64 25.378274616079494 22.80374887082204 25.03952122854562 26.778455284552845 65-69 29.905451149760708 12.44309559939302 30.477413330220614 27.17403992062566 70-74 27.579087983484126 16.32764588013844 29.837877223875157 26.255388912502276 75-79 19.158163265306122 24.668367346938773 26.007653061224488 30.165816326530614 80-84 23.002764107058585 24.64100316861053 22.88815478999528 29.4680779343356 85-89 24.928815489749432 22.522779043280185 25.355922551252846 27.19248291571754 90-94 16.22277866302451 28.841338493057133 23.43880415813036 31.497078685787994 95-99 21.66366464712625 16.947764859996724 31.88963484525954 29.498935647617486 100-104 16.951857434169696 16.721340544374502 29.399769483110205 36.9270325383456 105-109 24.662475523034114 16.603112439451717 25.260228795217976 33.4741832422962 110-114 26.62715016271502 18.04974430497443 27.150162715016275 28.172942817294285 115-119 18.64561586638831 14.744258872651356 34.53810020876827 32.07202505219207 120-124 23.910482921083627 13.044758539458186 29.28445229681979 33.7603062426384 125-129 25.21339461588969 12.770847012475379 30.597504924491137 31.418253447143798 130-134 19.457943925233646 17.364485981308412 23.252336448598133 39.925233644859816 135-139 24.266666666666666 22.618181818181817 20.315151515151513 32.800000000000004 140-144 24.70983506414172 22.87721441661576 24.49602932193036 27.916921197312156 145-149 20.599379951774026 24.078539441956597 23.389596968653116 31.93248363761626 150-154 22.0023282887078 20.87698874660458 25.99922390376407 31.121459060923556 155-159 28.719101123595504 22.831460674157302 22.382022471910112 26.06741573033708 160-164 30.303030303030305 23.593073593073594 19.642857142857142 26.461038961038962 165-169 30.8411214953271 22.963951935914555 19.893190921228303 26.301735647530037 170-174 25.573491928632112 24.63891248937978 20.135938827527614 29.651656754460493 175-179 26.914660831509845 15.317286652078774 24.72647702407002 33.04157549234136 180-184 29.268292682926827 12.330623306233063 23.712737127371277 34.68834688346883 185-189 27.378964941569283 20.534223706176963 17.195325542570952 34.891485809682806 190-194 20.801526717557252 22.519083969465647 20.99236641221374 35.68702290076336 195-199 23.340961098398168 15.789473684210526 26.773455377574372 34.09610983981693 200-204 25.761772853185594 14.127423822714682 26.31578947368421 33.795013850415515 205-209 23.3201581027668 26.48221343873518 16.99604743083004 33.201581027667984 210-214 21.85792349726776 19.12568306010929 25.136612021857925 33.87978142076503 215-219 29.37062937062937 20.97902097902098 20.97902097902098 28.671328671328673 220-224 21.35922330097087 25.24271844660194 17.475728155339805 35.92233009708738 225-229 31.645569620253166 31.645569620253166 8.860759493670885 27.848101265822784 230-234 19.230769230769234 30.76923076923077 23.076923076923077 26.923076923076923 235-239 26.31578947368421 21.052631578947366 23.684210526315788 28.947368421052634 240-244 27.77777777777778 33.33333333333333 22.22222222222222 16.666666666666664 245-248 25.0 25.0 25.0 25.0 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 1.0 27 2.0 28 1.0 29 0.0 30 0.0 31 0.0 32 0.5 33 1.0 34 10.5 35 12.0 36 4.0 37 5.0 38 5.499999999999999 39 4.5 40 4.666666666666666 41 5.0 42 7.333333333333333 43 16.166666666666668 44 43.5 45 114.33333333333334 46 169.5 47 221.5 48 185.5 49 75.5 50 41.5 51 32.5 52 27.5 53 40.33333333333333 54 59.833333333333336 55 66.5 56 53.5 57 46.0 58 72.5 59 115.0 60 185.0 61 280.0 62 411.66666666666663 63 544.1666666666669 64 434.00000000000034 65 284.5 66 246.16666666666663 67 179.66666666666669 68 163.0 69 128.5 70 79.5 71 48.0 72 36.0 73 37.0 74 39.5 75 61.0 76 72.5 77 41.0 78 15.5 79 9.0 80 2.0 81 3.0 82 6.5 83 5.5 84 3.0 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-154 0.0 155-159 0.0 160-164 0.0 165-169 0.0 170-174 0.0 175-179 0.0 180-184 0.0 185-189 0.0 190-194 0.0 195-199 0.0 200-204 0.0 205-209 0.0 210-214 0.0 215-219 0.0 220-224 0.0 225-229 0.0 230-234 0.0 235-239 0.0 240-244 0.0 245-248 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 25-29 15.0 30-34 37.0 35-39 37.0 40-44 42.0 45-49 75.0 50-54 108.0 55-59 103.0 60-64 97.0 65-69 149.0 70-74 119.0 75-79 196.0 80-84 144.0 85-89 162.0 90-94 214.0 95-99 161.0 100-104 254.0 105-109 283.0 110-114 208.0 115-119 169.0 120-124 146.0 125-129 169.0 130-134 121.0 135-139 299.0 140-144 70.0 145-149 91.0 150-154 52.0 155-159 85.0 160-164 61.0 165-169 77.0 170-174 56.0 175-179 36.0 180-184 38.0 185-189 15.0 190-194 17.0 195-199 12.0 200-204 26.0 205-209 15.0 210-214 10.0 215-219 8.0 220-224 6.0 225-229 5.0 230-234 3.0 235-239 5.0 240-244 3.0 245-249 1.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.725 #Duplication Level Percentage of deduplicated Percentage of total 1 78.96528250510552 28.999999999999996 2 7.964601769911504 5.8500000000000005 3 2.859087814840027 3.15 4 2.042205582028591 3.0 5 1.2253233492171545 2.25 6 0.9530292716133424 2.1 7 0.9530292716133424 2.45 8 0.4084411164057182 1.2 9 0.6807351940095302 2.25 >10 3.4036759700476518 22.75 >50 0.3403675970047651 8.425 >100 0.2042205582028591 17.575 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGCGTCCTCTGCTGGGGGCTGTGACGTTGCCCATGAGACTGCGATTCTCG 354 8.85 No Hit AACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGA 211 5.2749999999999995 No Hit GTGTTCGCGCCCACGGACAACGCGTTCACGAGCCTGGCTTCCGGCACGCT 138 3.45 No Hit TGCTGGGGGCTGTGACGTTGCCCATGAGACTGCGATTCTCGGTCTACACG 99 2.475 No Hit TAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGGCTG 82 2.0500000000000003 No Hit ACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGAC 53 1.325 No Hit AGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACACGCTC 52 1.3 No Hit AGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAGC 51 1.275 No Hit TCTGCTGGGGGCTGTGACGTTGCCCATGAGACTGCGATTCTCGGTCTACA 47 1.175 No Hit AACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCG 45 1.125 No Hit CGTTCACGAGCCTGGCTTCCGGCACGCTCAACTCGCTTTCGGACAGCCAG 42 1.05 No Hit CAGAAGAACTCGCTGGTGCAGTTCCACGTGCTGTCCACGGCGGTGCCCAT 40 1.0 No Hit CGTCGTCGCGGTGGCCATGGCATTGGCGTCCTGCGTCCTCTGCTGGGGGC 39 0.975 No Hit GACACGGATTCCACCGCGTCCCGGGCAGCCGGAGGTGGTGGGGGTGGCGG 38 0.95 No Hit GGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCA 28 0.7000000000000001 No Hit GGACAACGCGTTCACGAGCCTGGCTTCCGGCACGCTCAACTCGCTTTCGG 27 0.675 No Hit CGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGC 25 0.625 No Hit CAGCAGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACAC 25 0.625 No Hit GAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAG 24 0.6 No Hit GCTCCCAGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTC 22 0.5499999999999999 No Hit AACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGT 21 0.525 No Hit GAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCG 19 0.475 No Hit AGAGATGAGGAGTTGGTGTAACCAGCTCAACAGCTCCCAGACGGGCGGGC 19 0.475 No Hit TCCTCTGCTGGGGGCTGTGACGTTGCCCATGAGACTGCGATTCTCGGTCT 18 0.44999999999999996 No Hit TGCGTCCTCTGCTGGGGGGCTGTGACGTTGCCCATGAGACTGCGATTCTC 17 0.42500000000000004 No Hit AGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACA 17 0.42500000000000004 No Hit CGGGAGCAGCTCCCCGGGGGAGTACCCGCTCAACGTCACCGCCACCGGCC 17 0.42500000000000004 No Hit AAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACAC 16 0.4 No Hit CACGCTCAACTCGCTTTCGGACAGCCAGAAGAACTCGCTGGTGCAGTTCC 16 0.4 No Hit CCCAGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCACG 15 0.375 No Hit GTGGCGGCGTCGTCGCGGTGGCCATGGCATTGGCGTCCTGCGTCCTCTGC 14 0.35000000000000003 No Hit TGCTGGGGGGCTGTGACGTTGCCCATGAGACTGCGATTCTCGGTCTACAC 14 0.35000000000000003 No Hit AGCAGCTCCCCGGGGGAGTACCCGCTCAACGTCACCGCCACCGGCCAGCA 14 0.35000000000000003 No Hit TGTTCGCGCCCACGGACAACGCGTTCACGAGCCTGGCTTCCGGCACGCTC 14 0.35000000000000003 No Hit ACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGAC 14 0.35000000000000003 No Hit AACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACA 13 0.325 No Hit AACGACTCCAGCCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAG 13 0.325 No Hit CAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACACGCTCTTCA 13 0.325 No Hit ACCGGCCAGCAGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGA 13 0.325 No Hit ACAACCAGCTCAACAGCTCCCAGACGGGCGGGCTCACGGTGTTCGCGCCC 12 0.3 No Hit CAGTTCGACACCGTGAGCAACCCGCTCAGGACGCAGGCCGGGAGCAGCTC 12 0.3 No Hit CAGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAG 12 0.3 No Hit AGCAGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACACG 12 0.3 No Hit TTCCACGGGCGTCGCCGCCCAAATCGACAACCAGCTCAACAGCTCCCAGA 12 0.3 No Hit TGCGTCCTCTGCTGGGGGCTGTGACGTTGCCATGAGACTGCGATTCTCGG 12 0.3 No Hit AACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGC 12 0.3 No Hit AACACGTTCATCCGGCTGCTGCGTTCCACGGGCGTCGCCGCCCAAATCGACAACGCACCGGCAGCCGGAACGACC 12 0.3 No Hit ACAACGCGTTCACGAGCCTGGCTTCCGGCACGCTCAACTCGCTTTCGGAC 11 0.27499999999999997 No Hit GTGTTCGCGCCCACGGACACGCGTTCACGAGCCTGGCTTCCGGCACGCTC 11 0.27499999999999997 No Hit TGCGTCCTCTGCTGGGGGCTGTGACGTTTGCCCATGAGACTGCGATTCTC 11 0.27499999999999997 No Hit CGTTCCACGGGCGTCGCCGCCCAAATCGACAACCAGCTCAACAGCTCCCA 11 0.27499999999999997 No Hit AACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAAACGACCAAG 11 0.27499999999999997 No Hit TAACAGGCGTGCTAGCGAAGCCGGGCAGTTCAACACGTTCATCCGGCTGC 10 0.25 No Hit AGGCGACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTTGC 10 0.25 No Hit GAAGAAGGCGGACGCGCCGGCCCCCGCGCCGCTGGGTCCGGCCAAGAAGACGCCGG 10 0.25 No Hit ACAGCTCCCAGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCG 10 0.25 No Hit GGGGGAGTACCCGCTCAACGTCACCGCCACCGGCCAGCAGGTCAACATCT 10 0.25 No Hit AGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACACACGCTCT 10 0.25 No Hit GACAACGCGTTCACGAGCCTGGCTTCCGGCACGCTTAACTCGCTTTCGGA 9 0.22499999999999998 No Hit CGGCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTA 9 0.22499999999999998 No Hit TGAAGGGCTCGCCGTCTACGAGGGGCTCGCCGTTGCTCTGTGGGCGCGAG 9 0.22499999999999998 No Hit ACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCAT 9 0.22499999999999998 No Hit ACGCAGGCCGGGAGCAGCTCCCCGGGGGAGTACCCGCTCAACGTCACCGCCACCGGCCAGCAGG 9 0.22499999999999998 No Hit GCCTGGCTTCCGGCACGCTCAACTCGCTTTCGGACAGCCAGAAGAACTCG 9 0.22499999999999998 No Hit TGCTGGGGCTGTGACGTTGCCCATGAGACTGCGATTCTCGGTCTACACGT 9 0.22499999999999998 No Hit AAGCTCTCCACCAGATAGATCACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTACACCACCAG 9 0.22499999999999998 No Hit AACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGG 9 0.22499999999999998 No Hit TCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAGCCTGGCTTCCGGC 9 0.22499999999999998 No Hit CAGTTCGACACCGTGAGCAACCCGCTCAGGACGCAGGCCGGGAGCAGCTCCCCGGGGGAGTACCCGCTCAAC 8 0.2 No Hit GGGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCACGGGCGTCGCCGC 8 0.2 No Hit GACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGA 8 0.2 No Hit CGTCGACAACACGCTCTTCACCGGCGACCAGCTCGTGGTCTACCAGGTCA 8 0.2 No Hit GGGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCACGGGCGTCTGGAA 8 0.2 No Hit AACGACTCCAGCCCCAGCGGTTCCGGCACCGGCAGCCGGAACGACCAAGA 8 0.2 No Hit GCGTGGCAGGCAGCTCTGCAGTACCATTTATGACACTCGTCGTCTAGTTG 7 0.17500000000000002 No Hit TCGACAACACGCTCTTCACCGGCGACCAGCTCGTGGTCTACCAGGTCAAC 7 0.17500000000000002 No Hit CGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTC 7 0.17500000000000002 No Hit AGCAACCCGCTCAGGACGCAGGCCGGGAGCAGCTCCCCGGGGGAGTAC 7 0.17500000000000002 No Hit GCTCCCAGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACACGCGTTCA 7 0.17500000000000002 No Hit AGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACACGCTCTTCACCGGCGACCAGCTCG 7 0.17500000000000002 No Hit ACTCCAATCTTGTTATAACTAAGCCACAGCTAAGCTCGCAAGCTCTCCAC 7 0.17500000000000002 No Hit TGTCCACGGCGGTGCCCATGTCGCAGTTCGACACCGTGAGCAACCCGCTC 7 0.17500000000000002 No Hit CAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCC 7 0.17500000000000002 No Hit ACCACCAATGCTACGTGCTCTGCTTGCATCTCACTCCAATCTTGTTATAA 7 0.17500000000000002 No Hit CATGGCATTGGCGTCCTGCGTCCTCTGCTGGGGGCTGTGACGTTGCCCAT 7 0.17500000000000002 No Hit AGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAGCCTGGCTTCCG 7 0.17500000000000002 No Hit AGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACACGCGTTCACGAGCC 7 0.17500000000000002 No Hit AACGACTCCAGCCCCAGCGGCTCCGGCACGGCAGCCGGAACGACCAAGAC 7 0.17500000000000002 No Hit AACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAAAACGACCAA 6 0.15 No Hit CGGACAACGCGTTCACGAGCCTGGCTTCCGGCACGCTCAACTCGCTTTCGGACAGCC 6 0.15 No Hit CGTTCCACGGCGTCGCCGCCCCAAATCGACAACCAGCTCAACAGCTCCCA 6 0.15 No Hit GGCGTCGTCGCGGTGGCCATGGCATTGGCGTCCTGCGTCCTCTGCTGGGG 6 0.15 No Hit CGGACAACGCGTTCACGAGCCTGGCTTCCGGCACGCTCAACTCGCTTTCG 6 0.15 No Hit AACATAACAGGCGTGCTAGCGAAAAGCCGGGCAGTTCAACACGTTCATCC 6 0.15 No Hit TGCGTCCTCTGCTGGGGCTGTGACGTTGCCCATGAGACTGCGATTCTCGG 6 0.15 No Hit CTGCTGCGTTCCACAGGCGTCGCCGCCCAAATCGACAACCAGCTCAACAG 6 0.15 No Hit TGCGTCCTCTGCTGGGGGCTGTGACGTTGCCCATGAGAGCTGCGATTCTC 6 0.15 No Hit GGGCGCGTGGCAGGCAGCTCTGCAGTACCATTTATGACACTCGTCGTCTA 6 0.15 No Hit GTTCCACGGGCGTCGCCGCCCCAAATCGACAACCAGCTCAACAGCTCCCA 6 0.15 No Hit AGTTCGACACCGTGAGCAACCCGCTCAGGACGCAGGCCGGGAGCAGCAGT 6 0.15 No Hit CCAGCAGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACA 6 0.15 No Hit ACTCTCGCATTTGTCCTCGCTGATAGCTAGAGTTTCACGATTACACCACC 6 0.15 No Hit ACACCAGCTCAACAGCTCCCAGACGGGCGGGCTCACGGTGTTCGCGCCCA 5 0.125 No Hit GCGGCGCCAACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAAC 5 0.125 No Hit AACAGCTCCCAGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGC 5 0.125 No Hit CATTGGCGTCCTGCGTCCTCTGCTGGGGGCTGTGACGTTGCCCATGAGAC 5 0.125 No Hit AACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAAGCCGGGCAG 5 0.125 No Hit AACGACTCCAGCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGAC 5 0.125 No Hit TGCTGCTCGTGATCTCAGCGGCGATCACGGCGTCGGCGCAGGGCCCAACG 5 0.125 No Hit ACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGA 5 0.125 No Hit GAGGTGGTGGGGGTGGCGGCGTCGTCGCGGTGGCCATGGCATTGGCGTCC 5 0.125 No Hit TGCTGGGGGCTGTGACGTTTGCCCATGAGACTGCGATTCTCGGTCTACAC 5 0.125 No Hit AAGAAAAAAGGAGCGTGAGAGCCAAATGAATCGAAAGATTCATGTTTGGT 5 0.125 No Hit CGCCAACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACC 5 0.125 No Hit CAGAAGAACTCGCTGGTGCAGTTCCACGTGCTGTCCACGGCGGTGCCCATGTCGCAGTTCGACACCG 5 0.125 No Hit GCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGC 5 0.125 No Hit GGCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAG 5 0.125 No Hit AACACGTTCATCCGGCTGCTGCGTTCCACGGGCGTCGCCGCCCAAATCGA 5 0.125 No Hit GCGTGGCAGGCAGCTCTGCAGTACCATTTATGACACTCGTCTCTAGTTGC 5 0.125 No Hit AGTTCGACACCGTGAGCAACCCGCTCAGGACGCAGGCCGGGAGCAGCTCC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-14 0.0 0.0 0.0 0.0 0.0 15-19 0.0 0.0 0.0 0.0 0.0 20-24 0.0 0.0 0.0 0.0 0.0 25-29 0.0 0.0 0.0 0.0 0.0 30-34 0.0 0.0 0.0 0.0 0.0 35-39 0.0 0.0 0.0 0.0 0.0 40-44 0.0 0.0 0.0 0.0 0.0 45-49 0.0 0.0 0.0 0.0 0.0 50-54 0.0 0.0 0.0 0.0 0.0 55-59 0.0 0.0 0.0 0.0 0.0 60-64 0.0 0.0 0.0 0.0 0.0 65-69 0.0 0.0 0.0 0.0 0.0 70-74 0.0 0.0 0.0 0.0 0.0 75-79 0.0 0.0 0.0 0.0 0.0 80-84 0.0 0.0 0.0 0.0 0.0 85-89 0.0 0.0 0.0 0.0 0.0 90-94 0.0 0.0 0.0 0.0 0.0 95-99 0.0 0.0 0.0 0.0 0.0 100-104 0.0 0.0 0.0 0.0 0.0 105-109 0.0 0.0 0.0 0.0 0.0 110-114 0.0 0.0 0.0 0.0 0.0 115-119 0.0 0.0 0.0 0.0 0.0 120-124 0.0 0.0 0.0 0.0 0.0 125-129 0.0 0.0 0.0 0.0 0.0 130-134 0.0 0.0 0.0 0.0 0.0 135-139 0.0 0.0 0.0 0.0 0.0 140-144 0.0 0.0 0.0 0.0 0.0 145-149 0.0 0.0 0.0 0.0 0.0 150-154 0.0 0.0 0.0 0.0 0.0 155-159 0.0 0.0 0.0 0.0 0.0 160-164 0.0 0.0 0.0 0.0 0.0 165-169 0.0 0.0 0.0 0.0 0.0 170-174 0.0 0.0 0.0 0.0 0.0 175-179 0.0 0.0 0.0 0.0 0.0 180-184 0.0 0.0 0.0 0.0 0.0 185-189 0.0 0.0 0.0 0.0 0.0 190-194 0.0 0.0 0.0 0.0 0.0 195-199 0.0 0.0 0.0 0.0 0.0 200-204 0.0 0.0 0.0 0.0 0.0 205-209 0.0 0.0 0.0 0.0 0.0 210-214 0.0 0.0 0.0 0.0 0.0 215-219 0.0 0.0 0.0 0.0 0.0 220-224 0.0 0.0 0.0 0.0 0.0 225-229 0.0 0.0 0.0 0.0 0.0 230-234 0.0 0.0 0.0 0.0 0.0 235-236 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCTTCG 5 6.485902E-4 2901.6667 210-212 TCGCTTC 5 6.485902E-4 2901.6667 210-212 GCTTCGG 5 6.485902E-4 2901.6667 210-212 ACCGGAC 5 0.001297081 2176.25 205-209 CACCGGA 5 0.001297081 2176.25 205-209 GTTCACC 5 0.009723639 870.5 200-204 AACGACT 50 0.0 97.93125 1 TCCGGCA 150 3.2820954E-4 96.72222 195-199 GTCCTCT 85 0.0 89.61029 4 CGTCCTC 85 0.0 89.61029 3 TGCGTCC 90 0.0 84.63194 1 TCCTCTG 85 0.0 83.20956 5 CAGCCCC 60 0.0 81.609375 9 CTCTGCT 80 0.0 81.609375 7 CCTCTGC 80 0.0 81.609375 6 ACGACTC 60 0.0 81.609375 2 GACTCCA 60 0.0 81.609375 4 CGACTCC 60 0.0 81.609375 3 GCGTCCT 100 0.0 76.16875 2 CCAGCCC 65 1.8189894E-12 75.33173 8 >>END_MODULE Read 1310284 spots for ERR1943000.sra Written 1310284 spots for ERR1943000.sra Read 1310284 spots for ERR1943000.sra Written 1310284 spots for ERR1943000.sra Read 1310284 spots for ERR1943000.sra Written 1310284 spots for ERR1943000.sra Read 1310284 spots for ERR1943000.sra Written 1310284 spots for ERR1943000.sra Read 1310284 spots for ERR1943000.sra Written 1310284 spots for ERR1943000.sra Read 1310284 spots for ERR1943000.sra Written 1310284 spots for ERR1943000.sra Read 1310284 spots for ERR1943000.sra Written 1310284 spots for ERR1943000.sra Read 1310284 spots for ERR1943000.sra Written 1310284 spots for ERR1943000.sra Read 1310284 spots for ERR1943000.sra Written 1310284 spots for ERR1943000.sra Read 1310284 spots for ERR1943000.sra Written 1310284 spots for ERR1943000.sra Read 1310284 spots for ERR1943000.sra Written 1310284 spots for ERR1943000.sra Read 1310284 spots for ERR1943000.sra Written 1310284 spots for ERR1943000.sra Read 1310284 spots for ERR1943000.sra Written 1310284 spots for ERR1943000.sra Read 1310284 spots for ERR1943000.sra Written 1310284 spots for ERR1943000.sra Read 1310284 spots for ERR1943000.sra Written 1310284 spots for ERR1943000.sra Read 1310284 spots for ERR1943000.sra Written 1310284 spots for ERR1943000.sra Read 1310284 spots for ERR1943000.sra Written 1310284 spots for ERR1943000.sra Read 1310284 spots for ERR1943000.sra Written 1310284 spots for ERR1943000.sra Read 1310296 spots for ERR1943000.sra Written 1310296 spots for ERR1943000.sra Read 1310284 spots for ERR1943000.sra Written 1310284 spots for ERR1943000.sra SRR ids: ['ERR1943000.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_1rr_do5l ERR1943000.sra spots: 26205692 blocks: [[1, 1310284], [1310285, 2620568], [2620569, 3930852], [3930853, 5241136], [5241137, 6551420], [6551421, 7861704], [7861705, 9171988], [9171989, 10482272], [10482273, 11792556], [11792557, 13102840], [13102841, 14413124], [14413125, 15723408], [15723409, 17033692], [17033693, 18343976], [18343977, 19654260], [19654261, 20964544], [20964545, 22274828], [22274829, 23585112], [23585113, 24895396], [24895397, 26205692]] ERR1943000 file size 7045515 ERR1943000 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR1943000 ERR1943000_1.fastq Input file: ERR1943000_1.fastq trimmed: ERR1943000-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Tue Dec 10 00:57:17 2024 >> started Tue Dec 10 00:57:35 2024 >> done (17.593s) 26205692 reads processed; of these: 252 ( 0.00%) short reads filtered out after trimming by size control 15 ( 0.00%) empty reads filtered out after trimming by size control 26205425 (100.00%) reads available; of these: 1566644 ( 5.98%) trimmed reads available after processing 24638781 (94.02%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 367 0.00% 19 511 0.00% 20 727 0.00% 21 1282 0.00% 22 1807 0.01% 23 2468 0.01% 24 6365 0.02% 25 30389 0.12% 26 31023 0.12% 27 32369 0.12% 28 32840 0.13% 29 33412 0.13% 30 33604 0.13% 31 33969 0.13% 32 34498 0.13% 33 34256 0.13% 34 34701 0.13% 35 35887 0.14% 36 38358 0.15% 37 36896 0.14% 38 37505 0.14% 39 37559 0.14% 40 38227 0.15% 41 38754 0.15% 42 40833 0.16% 43 41515 0.16% 44 42251 0.16% 45 43581 0.17% 46 45539 0.17% 47 46978 0.18% 48 48291 0.18% 49 50559 0.19% 50 52583 0.20% 51 54949 0.21% 52 57006 0.22% 53 59543 0.23% 54 59955 0.23% 55 62374 0.24% 56 65415 0.25% 57 70978 0.27% 58 72524 0.28% 59 74735 0.29% 60 77082 0.29% 61 83384 0.32% 62 92958 0.35% 63 108155 0.41% 64 102502 0.39% 65 96639 0.37% 66 98605 0.38% 67 102825 0.39% 68 107615 0.41% 69 116097 0.44% 70 120302 0.46% 71 123627 0.47% 72 127583 0.49% 73 130341 0.50% 74 145452 0.56% 75 148003 0.56% 76 159114 0.61% 77 174311 0.67% 78 165713 0.63% 79 174594 0.67% 80 176995 0.68% 81 177269 0.68% 82 183475 0.70% 83 186982 0.71% 84 196607 0.75% 85 199723 0.76% 86 211219 0.81% 87 216611 0.83% 88 215464 0.82% 89 217990 0.83% 90 242818 0.93% 91 233303 0.89% 92 233767 0.89% 93 246555 0.94% 94 250734 0.96% 95 262984 1.00% 96 266595 1.02% 97 264544 1.01% 98 279790 1.07% 99 277075 1.06% 100 283167 1.08% 101 292556 1.12% 102 289100 1.10% 103 287580 1.10% 104 294895 1.13% 105 295485 1.13% 106 292668 1.12% 107 292434 1.12% 108 294479 1.12% 109 303773 1.16% 110 314479 1.20% 111 338147 1.29% 112 315579 1.20% 113 298795 1.14% 114 300673 1.15% 115 297515 1.14% 116 292892 1.12% 117 297697 1.14% 118 298167 1.14% 119 300671 1.15% 120 294815 1.13% 121 290030 1.11% 122 290885 1.11% 123 280036 1.07% 124 281212 1.07% 125 270757 1.03% 126 270354 1.03% 127 263814 1.01% 128 256543 0.98% 129 259545 0.99% 130 256901 0.98% 131 248585 0.95% 132 242019 0.92% 133 234211 0.89% 134 234483 0.89% 135 241779 0.92% 136 232050 0.89% 137 228034 0.87% 138 226322 0.86% 139 220166 0.84% 140 227128 0.87% 141 228117 0.87% 142 205563 0.78% 143 196367 0.75% 144 194999 0.74% 145 193080 0.74% 146 192576 0.73% 147 179713 0.69% 148 179760 0.69% 149 172312 0.66% 150 166127 0.63% 151 157696 0.60% 152 153371 0.59% 153 151434 0.58% 154 152964 0.58% 155 143858 0.55% 156 137982 0.53% 157 145510 0.56% 158 131553 0.50% 159 129115 0.49% 160 126654 0.48% 161 128246 0.49% 162 128745 0.49% 163 134175 0.51% 164 125046 0.48% 165 112988 0.43% 166 104811 0.40% 167 100545 0.38% 168 97076 0.37% 169 95795 0.37% 170 89828 0.34% 171 87149 0.33% 172 82502 0.31% 173 78232 0.30% 174 74770 0.29% 175 70065 0.27% 176 68886 0.26% 177 70291 0.27% 178 68416 0.26% 179 62994 0.24% 180 60650 0.23% 181 58793 0.22% 182 55090 0.21% 183 53089 0.20% 184 51994 0.20% 185 48028 0.18% 186 46076 0.18% 187 43792 0.17% 188 41131 0.16% 189 39098 0.15% 190 36050 0.14% 191 34311 0.13% 192 32637 0.12% 193 31446 0.12% 194 30026 0.11% 195 28111 0.11% 196 26679 0.10% 197 25747 0.10% 198 24295 0.09% 199 23239 0.09% 200 22128 0.08% 201 21334 0.08% 202 20105 0.08% 203 18940 0.07% 204 18687 0.07% 205 18444 0.07% 206 17080 0.07% 207 16118 0.06% 208 14953 0.06% 209 13847 0.05% 210 13204 0.05% 211 12480 0.05% 212 12040 0.05% 213 11491 0.04% 214 10841 0.04% 215 9967 0.04% 216 9160 0.03% 217 8592 0.03% 218 7921 0.03% 219 7418 0.03% 220 7000 0.03% 221 6458 0.02% 222 6099 0.02% 223 5547 0.02% 224 5169 0.02% 225 4708 0.02% 226 4475 0.02% 227 4115 0.02% 228 3771 0.01% 229 3503 0.01% 230 3350 0.01% 231 3005 0.01% 232 2778 0.01% 233 2556 0.01% 234 2336 0.01% 235 2161 0.01% 236 2020 0.01% 237 1833 0.01% 238 1659 0.01% 239 1597 0.01% 240 1436 0.01% 241 1244 0.00% 242 1166 0.00% 243 1031 0.00% 244 914 0.00% 245 789 0.00% 246 763 0.00% 247 643 0.00% 248 632 0.00% 249 578 0.00% 250 515 0.00% 251 429 0.00% 252 424 0.00% 253 345 0.00% 254 294 0.00% 255 278 0.00% 256 247 0.00% 257 220 0.00% 258 204 0.00% 259 169 0.00% 260 155 0.00% 261 138 0.00% 262 120 0.00% 263 117 0.00% 264 113 0.00% 265 82 0.00% 266 69 0.00% 267 72 0.00% 268 68 0.00% 269 68 0.00% 270 42 0.00% 271 42 0.00% 272 34 0.00% 273 36 0.00% 274 21 0.00% 275 33 0.00% 276 22 0.00% 277 24 0.00% 278 14 0.00% 279 17 0.00% 280 13 0.00% 281 14 0.00% 282 9 0.00% 283 6 0.00% 284 12 0.00% 285 11 0.00% 286 6 0.00% 287 4 0.00% 288 6 0.00% 289 0 0.00% 290 4 0.00% 291 3 0.00% 292 2 0.00% 293 5 0.00% 294 2 0.00% 295 1 0.00% 296 3 0.00% 297 1 0.00% 298 0 0.00% 299 2 0.00% 300 2 0.00% 301 2 0.00% 302 1 0.00% 303 1 0.00% 304 1 0.00% 305 2 0.00% 306 3 0.00% 307 1 0.00% 308 0 0.00% 309 3 0.00% 310 1 0.00% 311 0 0.00% 312 1 0.00% 313 2 0.00% 314 1 0.00% 315 0 0.00% 316 0 0.00% 317 0 0.00% 318 0 0.00% 319 0 0.00% 320 0 0.00% 321 1 0.00% 322 0 0.00% 323 0 0.00% 324 0 0.00% 325 0 0.00% 326 0 0.00% 327 0 0.00% 328 0 0.00% 329 0 0.00% 330 0 0.00% 331 0 0.00% 332 0 0.00% 333 0 0.00% 334 0 0.00% 335 1 0.00% 336 0 0.00% 337 1 0.00% 26205425 reads passed initial QC criterion=sequence-density sequence-density=2.46 sequence-density-rank=1 fanout-score=5.06 fanout-score-rank=21 prefix-density=10.90 prefix-fanout=1.1 sequence=GGGGGCTCGAAA criterion=fanout-score sequence-density=0.39 sequence-density-rank=28 fanout-score=33.16 fanout-score-rank=1 prefix-density=12.74 prefix-fanout=1.0 sequence=CTAGTCTCAAAACCATAAAC Potential 3prime adapter identified. Now checking if in reference sequence Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192. 1 reads; of these: 1 (100.00%) were unpaired; of these: 0 (0.00%) aligned 0 times 0 (0.00%) aligned exactly 1 time 1 (100.00%) aligned >1 times 100.00% overall alignment rate Potential adapter found in reference sequence. Continuing without clipping. Started job on | Dec 10 00:57:54 Started mapping on | Dec 10 00:57:54 Finished on | Dec 10 00:59:06 Mapping speed, Million of reads per hour | 1310.27 Number of input reads | 26205425 Average input read length | 115 UNIQUE READS: Uniquely mapped reads number | 17548620 Uniquely mapped reads % | 66.97% Average mapped length | 111.90 Number of splices: Total | 5583782 Number of splices: Annotated (sjdb) | 5163734 Number of splices: GT/AG | 5378569 Number of splices: GC/AG | 68881 Number of splices: AT/AC | 3368 Number of splices: Non-canonical | 132964 Mismatch rate per base, % | 0.66% Deletion rate per base | 0.32% Deletion average length | 1.14 Insertion rate per base | 0.55% Insertion average length | 1.29 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1577314 % of reads mapped to multiple loci | 6.02% Number of reads mapped to too many loci | 2016687 % of reads mapped to too many loci | 7.70% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 11.58% % of reads unmapped: other | 7.74% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 7079491 7079491 7079491 N_multimapping 1577314 1577314 1577314 N_noFeature 803829 923631 17084645 N_ambiguous 397550 59835 1730 UnstrandedReadsAssigned:16347241 PositiveStrandReadsAssigned:16565154 NegativeStrandReadsAssigned:462245 Dataset is classified positive stranded MeadianReadLen=115 20thPercentileLength=87 echo kmer=83 ERR1943000 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR1943000-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 26,205,425 reads, 18,102,917 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,211 rounds 52973 ERR1943000.ke.tsv 35125 ERR1943000.se.tsv 88098 total ==> ERR1943000.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 134.524 12.521 PNS24247 1044 945 0 0 PNS24249 1928 1829 16.518 0.703571 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 212.958 12.0922 PNS24243 293 194 0 0 KQK14069 1603 1504 7902.14 409.319 KQK14071 474 375 72.9779 15.1609 ==> ERR1943000.se.tsv <== BRADI_1g14170v3 7889 BRADI_1g53295v3 48 BRADI_1g59795v3 210 BRADI_1g07683v3 0 BRADI_1g00485v3 1 BRADI_1g20270v3 787 BRADI_1g74790v3 303 BRADI_1g09890v3 0 BRADI_1g77505v3 648 BRADI_1g48960v3 0 ERR1943000 completed mapping pipeline successfully