Starting /dee2/code/volunteer_pipeline.sh ERR1943001 current disk space = 1523672621056 free memory = 1569091804 ERR1943001 SRAfilesize 717cf26414909c57505251a7ef5a9634 ERR1943001.sra ERR1943001.sra file validated ERR1943001 is single end ERR1943001 is conventional basespace ERR1943001 read1 length is 25-274 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1943001_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 25-274 %GC 54 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.74425 27.0 24.0 28.0 21.0 29.0 2 25.223 27.0 23.0 28.0 20.0 29.0 3 25.27125 27.0 23.0 28.0 20.0 29.0 4 25.27925 27.0 23.0 28.0 20.0 29.0 5 25.03625 27.0 23.0 28.0 20.0 29.0 6 25.08475 27.0 23.0 28.0 20.0 29.0 7 24.2605 24.0 22.0 27.0 19.0 29.0 8 25.21325 27.0 23.0 28.0 20.0 29.0 9 24.5595 27.0 23.0 28.0 17.0 29.0 10-14 24.67215 26.2 22.8 27.8 19.2 28.8 15-19 24.3821 25.8 22.2 27.8 18.4 28.8 20-24 24.513599999999997 26.0 22.8 27.8 18.6 28.8 25-29 24.32694644357744 25.8 22.6 27.8 18.2 28.8 30-34 24.31005909971206 25.4 22.6 27.8 18.2 28.8 35-39 24.675344906660335 26.2 23.0 28.0 19.2 28.8 40-44 24.05647633549588 25.6 22.2 27.4 16.0 28.4 45-49 24.010321382102035 25.2 22.0 27.4 16.6 28.0 50-54 24.141854027184877 25.6 22.2 27.6 17.8 28.0 55-59 24.28739702173381 25.8 22.4 27.8 18.0 28.2 60-64 23.939873959571486 25.0 21.8 27.2 17.2 28.0 65-69 23.81588414357592 24.8 21.6 27.4 17.0 28.0 70-74 22.648090369284027 24.0 20.0 27.0 13.2 28.0 75-79 23.147120185682002 24.2 20.8 27.0 15.4 28.0 80-84 23.153010676342596 24.6 20.6 27.0 14.4 28.0 85-89 22.71314674821293 23.6 19.8 27.0 13.8 28.0 90-94 22.903693114574587 24.0 20.2 27.0 14.4 28.0 95-99 22.946739792780278 24.2 20.2 27.0 14.0 28.0 100-104 22.494557362208297 23.4 19.8 26.8 13.8 28.0 105-109 22.585634961562207 23.6 20.0 27.0 14.0 28.0 110-114 22.816831357127136 24.0 20.0 27.0 14.0 28.0 115-119 22.178109291866175 23.0 19.2 26.8 13.4 28.0 120-124 22.676549735370475 23.8 20.4 27.0 13.6 28.0 125-129 23.402194414155865 24.6 21.2 27.0 15.0 28.0 130-134 23.075827622686838 24.0 20.6 27.0 15.2 28.0 135-139 22.181173585852598 23.2 19.8 26.6 13.4 28.0 140-144 22.329131897662517 23.2 19.4 26.8 13.8 28.0 145-149 22.299478101407708 23.4 20.0 26.4 13.6 28.0 150-154 21.400298491600257 22.8 18.4 25.8 12.8 27.0 155-159 21.200072376840502 22.2 18.0 25.6 12.8 27.6 160-164 21.041460462516596 22.0 18.2 25.0 13.0 27.0 165-169 20.841452084652012 22.0 17.8 25.0 12.2 27.0 170-174 21.157807570726117 22.0 18.2 25.2 12.6 27.0 175-179 20.94111638237912 22.0 18.0 25.0 12.6 27.0 180-184 20.647882166427188 21.8 17.4 25.0 12.2 27.0 185-189 21.301283700710986 22.0 18.6 25.0 13.4 27.0 190-194 20.79641871277792 22.0 17.2 25.0 12.6 27.0 195-199 20.64662662085498 22.0 18.2 24.4 12.2 26.2 200-204 20.516798232064563 22.0 18.5 24.0 13.0 26.5 205-209 20.14525437207966 NaN NaN NaN NaN NaN 210-214 19.802181372549022 NaN NaN NaN NaN NaN 215-219 18.403296296296297 NaN NaN NaN NaN NaN 220-224 18.486596736596738 NaN NaN NaN NaN NaN 225-229 16.664040404040406 NaN NaN NaN NaN NaN 230-234 19.025 NaN NaN NaN NaN NaN 235-239 17.016666666666666 NaN NaN NaN NaN NaN 240-244 18.133333333333333 NaN NaN NaN NaN NaN 245-249 19.2 NaN NaN NaN NaN NaN 250-254 17.3 NaN NaN NaN NaN NaN 255-259 15.2 NaN NaN NaN NaN NaN 260-264 14.8 NaN NaN NaN NaN NaN 265-269 12.4 NaN NaN NaN NaN NaN 270-274 9.2 NaN NaN NaN NaN NaN >>END_MODULE >>Per sequence quality scores warn #Quality Count 16 13.0 17 51.0 18 99.0 19 172.0 20 295.0 21 391.0 22 437.0 23 487.0 24 601.0 25 704.0 26 642.0 27 108.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.825 25.724999999999998 38.800000000000004 8.649999999999999 2 52.300000000000004 19.2 8.35 20.150000000000002 3 19.625 16.175 11.924999999999999 52.275000000000006 4 42.35 17.175 17.05 23.425 5 18.975 25.224999999999998 28.299999999999997 27.500000000000004 6 23.724999999999998 15.5 13.200000000000001 47.575 7 19.900000000000002 16.775000000000002 23.799999999999997 39.525 8 21.425 12.950000000000001 33.95 31.674999999999997 9 16.25 13.900000000000002 23.150000000000002 46.7 10-14 26.705000000000002 16.68 27.560000000000002 29.054999999999996 15-19 34.839999999999996 18.505 13.48 33.175 20-24 29.26 21.355 22.275 27.11 25-29 27.93262482454381 15.580509324243033 26.724483657509523 29.76238219370363 30-34 21.34026341020336 19.367209971236818 20.90124640460211 38.391280213957714 35-39 29.523761169780666 25.929122664500404 17.028838342810722 27.518277822908203 40-44 26.65610304641178 18.973625025557144 26.221631568186467 28.148640359844613 45-49 21.518528116092252 17.709251101321584 28.39595750194351 32.376263280642654 50-54 26.834235489576074 17.610804437536846 26.716329921217643 28.838630151669438 55-59 22.30485319230981 19.996694761196494 23.83077177326062 33.86768027323308 60-64 23.735757983947913 18.684402536902958 28.33810405792221 29.241735421226917 65-69 24.11846047476377 13.626411615579626 36.67319658907582 25.581931320580782 70-74 23.375532489350213 20.543589128217434 33.93532129357413 22.145557088858222 75-79 20.47303216118133 20.47303216118133 26.71130021273933 32.34263546489801 80-84 23.06385203581465 24.808836023789294 27.54721913600418 24.58009280439187 85-89 20.02070393374741 20.096618357487923 32.98136645962733 26.90131124913734 90-94 25.027124773960217 24.50632911392405 22.10488245931284 28.361663652802893 95-99 24.954001839926406 18.46059490953695 32.712358172339776 23.87304507819687 100-104 17.82236897807129 23.13944627889256 26.382186097705528 32.65599864533063 105-109 21.054158607350097 21.557059961315282 31.092843326885884 26.29593810444874 110-114 24.82876712328767 17.208904109589042 30.59717465753425 27.365154109589042 115-119 20.327405488163937 15.239665528206336 32.38723354139677 32.045695442232955 120-124 26.975876316140212 15.673730507796883 27.41570038651206 29.934692789550848 125-129 21.8612818261633 17.74948785484343 33.24553702077846 27.14369329821481 130-134 21.293580971000324 17.970022808732487 29.32551319648094 31.41088302378625 135-139 20.492470492470495 21.184371184371184 33.21123321123321 25.11192511192511 140-144 21.933621933621932 25.70947570947571 27.513227513227513 24.843674843674844 145-149 24.72 19.52 26.37333333333333 29.386666666666667 150-154 19.84665290474786 32.85166617516956 23.326452373930994 23.975228546151577 155-159 23.785761809713907 33.06719893546241 20.093147039254823 23.053892215568865 160-164 31.16935483870968 30.806451612903224 16.85483870967742 21.16935483870968 165-169 25.592885375494074 28.11264822134387 28.705533596837945 17.588932806324113 170-174 25.97826086956522 21.684782608695652 30.59782608695652 21.73913043478261 175-179 24.332909783989834 18.360864040660736 29.03430749682338 28.27191867852605 180-184 22.859327217125383 27.06422018348624 22.32415902140673 27.75229357798165 185-189 22.732012513034412 24.817518248175183 25.75599582898853 26.694473409801876 190-194 21.373056994818654 23.05699481865285 29.404145077720205 26.165803108808287 195-199 21.428571428571427 28.405315614617937 27.408637873754156 22.757475083056477 200-204 26.623376623376622 22.943722943722943 27.705627705627705 22.727272727272727 205-209 24.723247232472325 23.616236162361623 21.40221402214022 30.25830258302583 210-214 21.38364779874214 33.9622641509434 22.0125786163522 22.641509433962266 215-219 21.052631578947366 28.07017543859649 18.421052631578945 32.45614035087719 220-224 24.59016393442623 29.508196721311474 8.19672131147541 37.704918032786885 225-229 18.367346938775512 24.489795918367346 16.3265306122449 40.816326530612244 230-234 25.0 15.909090909090908 27.27272727272727 31.818181818181817 235-239 32.432432432432435 8.108108108108109 29.72972972972973 29.72972972972973 240-244 35.294117647058826 23.52941176470588 5.88235294117647 35.294117647058826 245-249 20.0 60.0 0.0 20.0 250-254 25.0 50.0 12.5 12.5 255-259 60.0 0.0 0.0 40.0 260-264 40.0 20.0 20.0 20.0 265-269 0.0 20.0 20.0 60.0 270-274 40.0 0.0 40.0 20.0 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 1.0 24 2.5 25 4.0 26 9.0 27 14.0 28 12.5 29 10.833333333333336 30 8.0 31 6.833333333333333 32 28.0 33 67.5 34 198.66666666666666 35 171.83333333333331 36 36.5 37 43.5 38 29.5 39 10.666666666666664 40 6.166666666666666 41 14.166666666666664 42 14.0 43 19.0 44 33.0 45 82.0 46 122.5 47 209.66666666666669 48 187.0 49 76.5 50 67.0 51 47.0 52 41.5 53 38.0 54 46.0 55 56.0 56 45.0 57 40.0 58 43.5 59 69.5 60 135.0 61 210.0 62 304.1666666666667 63 425.16666666666686 64 372.83333333333366 65 254.16666666666666 66 230.66666666666666 67 183.16666666666666 68 128.33333333333331 69 104.5 70 78.5 71 34.0 72 23.0 73 24.0 74 32.5 75 63.5 76 86.0 77 52.5 78 19.5 79 10.5 80 3.0 81 4.0 82 4.0 83 1.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-154 0.0 155-159 0.0 160-164 0.0 165-169 0.0 170-174 0.0 175-179 0.0 180-184 0.0 185-189 0.0 190-194 0.0 195-199 0.0 200-204 0.0 205-209 0.0 210-214 0.0 215-219 0.0 220-224 0.0 225-229 0.0 230-234 0.0 235-239 0.0 240-244 0.0 245-249 0.0 250-254 0.0 255-259 0.0 260-264 0.0 265-269 0.0 270-274 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 25-29 28.0 30-34 23.0 35-39 25.0 40-44 40.0 45-49 80.0 50-54 135.0 55-59 80.0 60-64 65.0 65-69 133.0 70-74 128.0 75-79 148.0 80-84 139.0 85-89 170.0 90-94 114.0 95-99 181.0 100-104 340.0 105-109 217.0 110-114 220.0 115-119 144.0 120-124 157.0 125-129 132.0 130-134 201.0 135-139 212.0 140-144 111.0 145-149 70.0 150-154 74.0 155-159 91.0 160-164 110.0 165-169 50.0 170-174 47.0 175-179 52.0 180-184 69.0 185-189 43.0 190-194 39.0 195-199 24.0 200-204 41.0 205-209 33.0 210-214 7.0 215-219 13.0 220-224 3.0 225-229 2.0 230-234 1.0 235-239 4.0 240-244 2.0 245-249 0.0 250-254 1.0 255-259 0.0 260-264 0.0 265-269 0.0 270-274 1.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.65 #Duplication Level Percentage of deduplicated Percentage of total 1 78.97033158813264 22.625 2 6.719022687609074 3.85 3 3.054101221640489 2.625 4 1.6579406631762654 1.9 5 0.6108202443280977 0.8750000000000001 6 0.7853403141361256 1.35 7 0.6108202443280977 1.225 8 0.4363001745200698 1.0 9 0.6980802792321117 1.7999999999999998 >10 5.671902268760907 32.2 >50 0.5235602094240838 11.924999999999999 >100 0.2617801047120419 18.625 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGCGTCCTCTGCTGGGGGCTGTGACGTTGCCCATGAGACTGCGATTCTCG 405 10.125 No Hit GGTCACTTTTAATTTCACAAATGTTTTCTTCCTAGTCAATTTTCTTTGAC 194 4.8500000000000005 No Hit AACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGA 146 3.65 No Hit TGCTGGGGGCTGTGACGTTGCCCATGAGACTGCGATTCTCGGTCTACACG 93 2.325 No Hit TGGTCACTTTTAATTTCACAAATGTTTTCTTCCTAGTCAATTTTCTTTGA 88 2.1999999999999997 No Hit ACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGA 84 2.1 No Hit ACAACGCGTTCACGAGCCTGGCTTCCGGCACGCTCAACTCGCTTTCGGAC 77 1.925 No Hit GACACGGATTCCACCGCGTCCCGGGCAGCCGGAGGTGGTGGGGGTGGCGG 74 1.8499999999999999 No Hit AGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACACGCTC 61 1.525 No Hit GTGTTCGCGCCCACGGACAACGCGTTCACGAGCCTGGCTTCCGGCACGCT 48 1.2 No Hit CAGAAGAACTCGCTGGTGCAGTTCCACGTGCTGTCCACGGCGGTGCCCAT 43 1.075 No Hit GTCACTTTTAATTTCACAAATGTTTTCTTCCTAGTCAATTTTCTTTGACT 40 1.0 No Hit GGGCTGTGACGTTGCCCATGAGACTGCGATTCTCGGTCTACACGTCGAGT 36 0.8999999999999999 No Hit TAAGCTCGCAAGCTCTCCACCAGATAGATCACTCTCGCATTTGTCCTCGC 36 0.8999999999999999 No Hit AGCCTGGCTTCCGGCACGCTCAACTCGCTTTCGGACAGCCAGAAGAACTC 36 0.8999999999999999 No Hit GAGTACCCGCTCAACGTCACCGCCACCGGCCAGCAGGTCAACATCTCCAC 35 0.8750000000000001 No Hit AGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACA 33 0.8250000000000001 No Hit TCACTTTTAATTTCACAAATGTTTTCTTCCTAGTCAATTTTCTTTGACTT 28 0.7000000000000001 No Hit GCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAGCCTGGCT 28 0.7000000000000001 No Hit TGCGTCCTCTGCTGGGGCTGTGACGTTGCCCATGAGACTGCGATTCTCGG 28 0.7000000000000001 No Hit ACAGCTCCCAGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCG 27 0.675 No Hit TCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAGCCTGGCTTCCGGC 27 0.675 No Hit CGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTC 26 0.65 No Hit TGGCCATCGCCGGGAAGAAGGCGGACGCGCCGTCGTCAATGCCACCGTCG 26 0.65 No Hit ACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGG 26 0.65 No Hit TTGGTCACTTTTAATTTCACAAATGTTTTCTTCCTAGTCAATTTTCTTTG 26 0.65 No Hit TAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGGCTG 26 0.65 No Hit TCTGCTGGGGGCTGTGACGTTGCCCATGAGACTGCGATTCTCGGTCTACA 25 0.625 No Hit GTCAATGCCACCGTCGACAACACGCTCTTCACCGGCGACCAGCTGCTGCT 25 0.625 No Hit AACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGT 24 0.6 No Hit GTCGACAACACGCTCTTCACCGGCGACCAGCTCGTGGTCTACCAGGTCAA 24 0.6 No Hit TGCTGGGGCTGTGACGTTGCCCATGAGACTGCGATTCTCGGTCTACACGT 24 0.6 No Hit CCCAAATCGACAACCAGCTCAACAGCTCCCAGACGGGCGGGCTCACGGTG 22 0.5499999999999999 No Hit TCCGGCACGCTCAACTCGCTTTCGGACAGCCAGAAGAACTCGCTGGTGCA 21 0.525 No Hit GCAGAGATCATTTCTAGTGCTGCTCGTGATCTCAGCGGCGATCACGGCGT 20 0.5 No Hit CGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAGCCTGGCTTCCGGCACGCTCAACTCGCTTTCGG 20 0.5 No Hit GTCCACGGCGGTGCCCATGTCGCAGTTCGACACCGTGAGCAACCCGCTCA 20 0.5 No Hit AGTTCCACGTGCTGTCCACGGCGGTGCCCATGTCGCAGTTCGACACCGTG 19 0.475 No Hit TGAGCAACCCGCTCAGGACGCAGGCCGGGAGCAGCTCCCCGGGGGAGTAC 19 0.475 No Hit AATGCCACCGTCGACAACACGCTCTTCACCGGCGACCAGCTCGTGGTCTACCAGGTCAACCAGGA 19 0.475 No Hit AACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCG 18 0.44999999999999996 No Hit TGCGATTCTCGGTCTACACGTCGAGTTTTTTTTGGTCACTTTTAATTTCA 18 0.44999999999999996 No Hit TGGGGGTGGCGGCGTCGTCGCGGTGGCCATGGCATTGGCGTCCTGCGTCC 18 0.44999999999999996 No Hit TCAACACGTTCATCCGGCTGCTGCGTTCCACGGGCGTCGCCGCCCAAATC 17 0.42500000000000004 No Hit CGACAACACGCTCTTCACCGGCGACCAGCTCGTGGTCTACCAGGTCAACC 17 0.42500000000000004 No Hit GAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAG 16 0.4 No Hit GGGGTCGTCAATGCCACCGTCGACAACACGCTCTTCACCGGCGACCAGCT 16 0.4 No Hit TCCACGTGCTGTCCACGGCGGTCACGCTCAACTCGCTTTCGGACAGCCAGAAG 14 0.35000000000000003 No Hit AGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACG 14 0.35000000000000003 No Hit TTCCACGGGCGTCGCCGCCCAAATCGACAACCAGCTCAACAGCTCCCAGA 13 0.325 No Hit AGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAGCCTG 13 0.325 No Hit TCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACACGCTCTTCACCGGCGACCAGCTCGTG 13 0.325 No Hit CTCCCAGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCA 13 0.325 No Hit AACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCG 12 0.3 No Hit AAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACAC 12 0.3 No Hit GGTCAACATCTCCACGAGTGCTGCTCGTGATCTCACGGCGATCACGGCGT 12 0.3 No Hit AATTTCACAAATGTTTTCTTCCTAGTCAATTTTCTTTGACTTGTTCTCTT 12 0.3 No Hit AGCCTGGCTTCCGGCACGCTCAACTCGCTTTCGGACAGCCAGAAGAACTCGCTGGTGCAGTTCCACGTGC 12 0.3 No Hit GCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGC 12 0.3 No Hit CCTCTGCTGGGGGCTGTGACGTTGCCCATGAGACTGCGATTCTCGGTCTA 12 0.3 No Hit CCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACATAAC 11 0.27499999999999997 No Hit AGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAG 11 0.27499999999999997 No Hit GGGGTCGTCAATGCCACCGTCGACAACACGCTCTTCACCGGCGACCAGCTCGTGGTCTACCAGGTCAACCA 11 0.27499999999999997 No Hit AGCTCAACAGCTCCCAGACGGGCGGGCTCACGGTGTTCGCGCCCACGGAC 11 0.27499999999999997 No Hit AGGTCAACATCTCCACGGGGTCGTCAATGCCACCGTCGACAACACGCTCT 11 0.27499999999999997 No Hit AGGGCCCAACGGCGGCGCCAACGACTCCAGCCCCAGCGGCTCCGGC 11 0.27499999999999997 No Hit GCGATTCTCGGTCTACACGTCGAGTTTTTTTTGGTCACTTTTAATTTCAC 11 0.27499999999999997 No Hit AGACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAGC 10 0.25 No Hit AACACGTTCATCCGGCTGCTGCGTTCCACGGCGTCGCCGCCCAATCGACA 10 0.25 No Hit TCCACGGGCGTCGCCGCCCAAATCGACAACCAGCTCAACAGCTCCCAGAC 10 0.25 No Hit TGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCACGGGC 10 0.25 No Hit ACCGTGAGCAACCCGCTCAGGACGCAGGCCGGGAGCAGCTCCCCGGGGGA 10 0.25 No Hit CACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGC 10 0.25 No Hit GGCACCGGCAGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAG 10 0.25 No Hit GGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCACGGGCGTCGCCGCC 9 0.22499999999999998 No Hit GGTGGCGGCGTCGTCGCGGTGGCCATGGCATTGGCGTCCTGCGTCCTCTG 9 0.22499999999999998 No Hit ACTTTTAATTTCACAAATGTTTTCTTCCTAGTCAATTTTCTTTGACTTG 9 0.22499999999999998 No Hit GACAACCAGCTCAACAGCTCCCAGACGGGCGGGCTCACGGTGTTCGCGCC 9 0.22499999999999998 No Hit ATTGGCGTCCTGCGTCCTCTGCTGGGGGCTGTGACGTTGCCCATGAGACT 9 0.22499999999999998 No Hit CAACGACTCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCAACA 9 0.22499999999999998 No Hit GACACGGATTCCACCGCGTCCCGGGCAGCCGGAGGTGGTGGGGGTGGGCG 9 0.22499999999999998 No Hit TGTTCGCGCCCACGGACAACGCGTTCACGAGCCTGGCTTCCGGCACGCTC 9 0.22499999999999998 No Hit CCACCGGCCAGCAGGTCAACATCTCCACGGGGGTCGTCAATGCCAC 8 0.2 No Hit GAAAGCCGGGCAGTTCAACACGTTCATCCGGCTGCTGCGTTCCACGGGCG 8 0.2 No Hit TCCAGCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAGACGACCA 8 0.2 No Hit GAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCATCCG 8 0.2 No Hit TGCGTTCCACGGGGCCCAAATCGACAACCAGCTCAACAGCTCCCAGACGG 8 0.2 No Hit ACCGGCAGCCGGAACGACCAAGACGCCAACATAACAGGCGTGCTAGCGAA 7 0.17500000000000002 No Hit TCGACAACACGCTCTTCACCGGCGACCAGCTCGTGGTCTACCAGGTCAAC 7 0.17500000000000002 No Hit GGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCA 7 0.17500000000000002 No Hit ACCAACATAACAGGCGTGCTAGCGAAAGCCGGGCAGTTCAACACGTTCAT 7 0.17500000000000002 No Hit GCCAGAAGAACTCGCTGGTGCAGTTCCACGTGCTGTCCACGGCGGTGCCCATGTCGCAGTTCGACACCG 7 0.17500000000000002 No Hit GACAACCAGCTCAACAGCTCCCAGACGGGTGGGCTCACGGTGTTCGCGCC 7 0.17500000000000002 No Hit TGCTGGGGGCTGTGACGTTGCCCATGAGACTGCGAGTTCTCGGTCTACAC 7 0.17500000000000002 No Hit GTTCAACACGTTCATCCGGCTGCTGCGTTCCACGGGCGTCGCCGCCCAAA 6 0.15 No Hit GCAGGTCAACATCTCCACGGGGGTCGTCAATGCCACCGTCGACAACACGC 6 0.15 No Hit CGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAGCCGCTCAA 6 0.15 No Hit GGTCACTTTTAATTTCACAATGTTTTCTTCCTAGTCAATTTTCTTTGACT 6 0.15 No Hit AGCCGGAACGACCAAGACGACCAACATAACAGGCGTGCTAGCGAAAGCCG 6 0.15 No Hit GGCTGTGACGTTGCCCATGAGACTGCGATTCTCGGTCTACACGTCGAGTT 6 0.15 No Hit AACGACTCCAGCCCCCAGCGGCTCCGGCACCGGCAGCCGGAACGACCAAG 6 0.15 No Hit GAGCAGCTCCCCGGGGGAGTACCCGCTCAACGTCACCGCCACCGGCCAGC 6 0.15 No Hit CGATTACACCACCAGAATGGAAGCCATGCAGAGATCATTTCTAGTGCTGC 6 0.15 No Hit TGCGTCCTCTGCTGGGGGCTGTGACGTTGCCCATGAGACTGCGATTCTCGGTCTACACGTCGAGTTTT 5 0.125 No Hit GCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAGCCTGGCTTCCGGC 5 0.125 No Hit AACCAGCTCAACAGCTCCCAGACGGGCGGGCTCACGGTGTTCGCGCCCAC 5 0.125 No Hit GACGGGCGGGCTCACGGTGTTCGCGCCCACGGACAACGCGTTCACGAGCC 5 0.125 No Hit TCCTCTGCTGGGGGCTGTGACGTTGCCCATGAGACTGCGATTCTCGGTCT 5 0.125 No Hit GCGATTCTCGGTCTACACGTCGAGTTTTTTTGGTCACTTTTAATTTCACA 5 0.125 No Hit TGGTCACTTTTAATTTCACAATGTTTTCTTCCTAGTCAATTTTCTTTGAC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-14 0.0 0.0 0.0 0.0 0.0 15-19 0.0 0.0 0.0 0.0 0.0 20-24 0.0 0.0 0.0 0.0 0.0 25-29 0.0 0.0 0.0 0.0 0.0 30-34 0.0 0.0 0.0 0.0 0.0 35-39 0.0 0.0 0.0 0.0 0.0 40-44 0.0 0.0 0.0 0.0 0.0 45-49 0.0 0.0 0.0 0.0 0.0 50-54 0.0 0.0 0.0 0.0 0.0 55-59 0.0 0.0 0.0 0.0 0.0 60-64 0.0 0.0 0.0 0.0 0.0 65-69 0.0 0.0 0.0 0.0 0.0 70-74 0.0 0.0 0.0 0.0 0.0 75-79 0.0 0.0 0.0 0.0 0.0 80-84 0.0 0.0 0.0 0.0 0.0 85-89 0.0 0.0 0.0 0.0 0.0 90-94 0.0 0.0 0.0 0.0 0.0 95-99 0.0 0.0 0.0 0.0 0.0 100-104 0.0 0.0 0.0 0.0 0.0 105-109 0.0 0.0 0.0 0.0 0.0 110-114 0.0 0.0 0.0 0.0 0.0 115-119 0.0 0.0 0.0 0.0 0.0 120-124 0.0 0.0 0.0 0.0 0.0 125-129 0.0 0.0 0.0 0.0 0.0 130-134 0.0 0.0 0.0 0.0 0.0 135-139 0.0 0.0 0.0 0.0 0.0 140-144 0.0 0.0 0.0 0.0 0.0 145-149 0.0 0.0 0.0 0.0 0.0 150-154 0.0 0.0 0.0 0.0 0.0 155-159 0.0 0.0 0.0 0.0 0.0 160-164 0.0 0.0 0.0 0.0 0.0 165-169 0.0 0.0 0.0 0.0 0.0 170-174 0.0 0.0 0.0 0.0 0.0 175-179 0.0 0.0 0.0 0.0 0.0 180-184 0.0 0.0 0.0 0.0 0.0 185-189 0.0 0.0 0.0 0.0 0.0 190-194 0.0 0.0 0.0 0.0 0.0 195-199 0.0 0.0 0.0 0.0 0.0 200-204 0.0 0.0 0.0 0.0 0.0 205-209 0.0 0.0 0.0 0.0 0.0 210-214 0.0 0.0 0.0 0.0 0.0 215-219 0.0 0.0 0.0 0.0 0.0 220-224 0.0 0.0 0.0 0.0 0.0 225-229 0.0 0.0 0.0 0.0 0.0 230-234 0.0 0.0 0.0 0.0 0.0 235-239 0.0 0.0 0.0 0.0 0.0 240-244 0.0 0.0 0.0 0.0 0.0 245-249 0.0 0.0 0.0 0.0 0.0 250-254 0.0 0.0 0.0 0.0 0.0 255-259 0.0 0.0 0.0 0.0 0.0 260-262 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAATCTG 5 0.008396459 931.0 175-179 TGAATCT 5 0.008396459 931.0 175-179 GCAGTTC 150 0.0 279.3 190-194 AAAAAAA 10 0.0043304795 167.58 155-159 TATGTAA 20 0.0018349988 149.625 170-174 AAATAAA 10 0.006456695 147.0 150-154 TGCTAAA 15 2.566297E-4 118.01409 145-149 AATGCTG 15 3.3837667E-4 110.25 140-144 CAGCCCC 25 2.1404048E-7 104.737495 9 CCAGCCC 25 2.1404048E-7 104.737495 8 AACGACT 25 2.1404048E-7 104.737495 1 CTCCAGC 25 2.1404048E-7 104.737495 6 ACTCCAG 25 2.1404048E-7 104.737495 5 TCCAGCC 25 2.1404048E-7 104.737495 7 ACGACTC 25 2.1404048E-7 104.737495 2 GACTCCA 25 2.1404048E-7 104.737495 4 CGACTCC 25 2.1404048E-7 104.737495 3 TGCGTCC 80 0.0 91.64531 1 GTCCTCT 80 0.0 91.64531 4 CGTCCTC 80 0.0 91.64531 3 >>END_MODULE Read 1056943 spots for ERR1943001.sra Written 1056943 spots for ERR1943001.sra Read 1056943 spots for ERR1943001.sra Written 1056943 spots for ERR1943001.sra Read 1056943 spots for ERR1943001.sra Written 1056943 spots for ERR1943001.sra Read 1056943 spots for ERR1943001.sra Written 1056943 spots for ERR1943001.sra Read 1056943 spots for ERR1943001.sra Written 1056943 spots for ERR1943001.sra Read 1056943 spots for ERR1943001.sra Written 1056943 spots for ERR1943001.sra Read 1056943 spots for ERR1943001.sra Written 1056943 spots for ERR1943001.sra Read 1056943 spots for ERR1943001.sra Written 1056943 spots for ERR1943001.sra Read 1056943 spots for ERR1943001.sra Written 1056943 spots for ERR1943001.sra Read 1056943 spots for ERR1943001.sra Written 1056943 spots for ERR1943001.sra Read 1056943 spots for ERR1943001.sra Written 1056943 spots for ERR1943001.sra Read 1056943 spots for ERR1943001.sra Written 1056943 spots for ERR1943001.sra Read 1056943 spots for ERR1943001.sra Written 1056943 spots for ERR1943001.sra Read 1056943 spots for ERR1943001.sra Written 1056943 spots for ERR1943001.sra Read 1056943 spots for ERR1943001.sra Written 1056943 spots for ERR1943001.sra Read 1056943 spots for ERR1943001.sra Written 1056943 spots for ERR1943001.sra Read 1056943 spots for ERR1943001.sra Written 1056943 spots for ERR1943001.sra Read 1056943 spots for ERR1943001.sra Written 1056943 spots for ERR1943001.sra Read 1056943 spots for ERR1943001.sra Written 1056943 spots for ERR1943001.sra Read 1056947 spots for ERR1943001.sra Written 1056947 spots for ERR1943001.sra SRR ids: ['ERR1943001.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_51v0hig0 ERR1943001.sra spots: 21138864 blocks: [[1, 1056943], [1056944, 2113886], [2113887, 3170829], [3170830, 4227772], [4227773, 5284715], [5284716, 6341658], [6341659, 7398601], [7398602, 8455544], [8455545, 9512487], [9512488, 10569430], [10569431, 11626373], [11626374, 12683316], [12683317, 13740259], [13740260, 14797202], [14797203, 15854145], [15854146, 16911088], [16911089, 17968031], [17968032, 19024974], [19024975, 20081917], [20081918, 21138864]] ERR1943001 file size 5806046 ERR1943001 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR1943001 ERR1943001_1.fastq Input file: ERR1943001_1.fastq trimmed: ERR1943001-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Tue Dec 10 00:56:27 2024 >> started Tue Dec 10 00:56:39 2024 >> done (11.319s) 21138864 reads processed; of these: 302 ( 0.00%) short reads filtered out after trimming by size control 0 ( 0.00%) empty reads filtered out after trimming by size control 21138562 (100.00%) reads available; of these: 1010320 ( 4.78%) trimmed reads available after processing 20128242 (95.22%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 305 0.00% 19 447 0.00% 20 769 0.00% 21 1300 0.01% 22 2119 0.01% 23 2343 0.01% 24 5600 0.03% 25 27861 0.13% 26 28572 0.14% 27 29524 0.14% 28 29217 0.14% 29 29680 0.14% 30 30226 0.14% 31 30488 0.14% 32 31234 0.15% 33 31772 0.15% 34 31980 0.15% 35 32972 0.16% 36 34830 0.16% 37 34663 0.16% 38 35789 0.17% 39 36106 0.17% 40 37380 0.18% 41 38318 0.18% 42 39737 0.19% 43 40405 0.19% 44 41659 0.20% 45 42560 0.20% 46 45463 0.22% 47 46012 0.22% 48 48266 0.23% 49 48609 0.23% 50 49990 0.24% 51 52837 0.25% 52 53383 0.25% 53 56550 0.27% 54 56653 0.27% 55 58377 0.28% 56 61618 0.29% 57 65221 0.31% 58 65790 0.31% 59 68258 0.32% 60 73946 0.35% 61 73272 0.35% 62 76865 0.36% 63 83112 0.39% 64 81960 0.39% 65 80429 0.38% 66 83483 0.39% 67 86543 0.41% 68 89500 0.42% 69 98147 0.46% 70 99442 0.47% 71 98724 0.47% 72 102112 0.48% 73 102036 0.48% 74 111090 0.53% 75 113610 0.54% 76 115923 0.55% 77 122497 0.58% 78 123649 0.58% 79 123435 0.58% 80 129855 0.61% 81 132104 0.62% 82 137140 0.65% 83 139372 0.66% 84 146495 0.69% 85 147270 0.70% 86 155101 0.73% 87 156526 0.74% 88 154264 0.73% 89 159515 0.75% 90 169183 0.80% 91 170751 0.81% 92 169738 0.80% 93 176165 0.83% 94 176418 0.83% 95 187026 0.88% 96 191409 0.91% 97 191069 0.90% 98 215297 1.02% 99 193824 0.92% 100 200377 0.95% 101 202695 0.96% 102 204089 0.97% 103 201346 0.95% 104 205046 0.97% 105 210376 1.00% 106 205462 0.97% 107 211867 1.00% 108 216392 1.02% 109 219176 1.04% 110 222993 1.05% 111 223083 1.06% 112 218837 1.04% 113 207925 0.98% 114 213110 1.01% 115 212324 1.00% 116 216369 1.02% 117 216784 1.03% 118 222745 1.05% 119 213818 1.01% 120 224106 1.06% 121 211686 1.00% 122 212068 1.00% 123 210036 0.99% 124 210638 1.00% 125 205129 0.97% 126 208672 0.99% 127 201319 0.95% 128 198170 0.94% 129 198256 0.94% 130 197423 0.93% 131 190711 0.90% 132 189442 0.90% 133 186486 0.88% 134 185889 0.88% 135 187368 0.89% 136 186040 0.88% 137 183067 0.87% 138 186038 0.88% 139 178540 0.84% 140 185697 0.88% 141 187244 0.89% 142 165901 0.78% 143 159629 0.76% 144 159791 0.76% 145 159970 0.76% 146 161852 0.77% 147 153039 0.72% 148 152587 0.72% 149 140682 0.67% 150 141036 0.67% 151 137524 0.65% 152 136362 0.65% 153 137097 0.65% 154 135497 0.64% 155 128332 0.61% 156 123138 0.58% 157 122011 0.58% 158 116494 0.55% 159 118680 0.56% 160 110034 0.52% 161 111515 0.53% 162 114400 0.54% 163 113385 0.54% 164 107413 0.51% 165 99646 0.47% 166 95553 0.45% 167 93646 0.44% 168 94247 0.45% 169 90075 0.43% 170 84346 0.40% 171 83573 0.40% 172 79819 0.38% 173 77906 0.37% 174 75365 0.36% 175 72081 0.34% 176 71052 0.34% 177 72264 0.34% 178 70905 0.34% 179 66564 0.31% 180 63211 0.30% 181 60509 0.29% 182 57987 0.27% 183 56102 0.27% 184 55008 0.26% 185 51383 0.24% 186 51358 0.24% 187 50415 0.24% 188 47066 0.22% 189 44877 0.21% 190 42773 0.20% 191 41191 0.19% 192 39757 0.19% 193 37903 0.18% 194 37296 0.18% 195 35368 0.17% 196 33885 0.16% 197 32725 0.15% 198 31693 0.15% 199 30254 0.14% 200 29075 0.14% 201 27797 0.13% 202 27174 0.13% 203 26410 0.12% 204 25875 0.12% 205 25434 0.12% 206 23720 0.11% 207 23024 0.11% 208 21673 0.10% 209 19805 0.09% 210 19119 0.09% 211 18811 0.09% 212 18385 0.09% 213 18744 0.09% 214 18045 0.09% 215 16111 0.08% 216 14573 0.07% 217 13480 0.06% 218 13009 0.06% 219 12674 0.06% 220 11526 0.05% 221 10953 0.05% 222 10170 0.05% 223 9656 0.05% 224 9196 0.04% 225 8393 0.04% 226 8154 0.04% 227 7746 0.04% 228 7180 0.03% 229 6853 0.03% 230 6426 0.03% 231 5933 0.03% 232 5769 0.03% 233 5374 0.03% 234 5096 0.02% 235 4628 0.02% 236 4220 0.02% 237 3974 0.02% 238 3797 0.02% 239 3534 0.02% 240 3239 0.02% 241 3166 0.01% 242 2855 0.01% 243 2754 0.01% 244 2487 0.01% 245 2316 0.01% 246 2114 0.01% 247 1962 0.01% 248 1829 0.01% 249 1680 0.01% 250 1548 0.01% 251 1365 0.01% 252 1295 0.01% 253 1164 0.01% 254 1050 0.00% 255 975 0.00% 256 870 0.00% 257 841 0.00% 258 744 0.00% 259 738 0.00% 260 604 0.00% 261 570 0.00% 262 494 0.00% 263 443 0.00% 264 408 0.00% 265 368 0.00% 266 345 0.00% 267 313 0.00% 268 250 0.00% 269 223 0.00% 270 206 0.00% 271 177 0.00% 272 182 0.00% 273 153 0.00% 274 118 0.00% 275 112 0.00% 276 101 0.00% 277 96 0.00% 278 77 0.00% 279 92 0.00% 280 63 0.00% 281 53 0.00% 282 46 0.00% 283 43 0.00% 284 25 0.00% 285 34 0.00% 286 29 0.00% 287 23 0.00% 288 16 0.00% 289 21 0.00% 290 14 0.00% 291 16 0.00% 292 11 0.00% 293 16 0.00% 294 11 0.00% 295 8 0.00% 296 8 0.00% 297 2 0.00% 298 5 0.00% 299 1 0.00% 300 5 0.00% 301 7 0.00% 302 1 0.00% 303 2 0.00% 304 2 0.00% 305 2 0.00% 306 0 0.00% 307 6 0.00% 308 2 0.00% 309 2 0.00% 310 3 0.00% 311 1 0.00% 312 3 0.00% 313 3 0.00% 314 2 0.00% 315 0 0.00% 316 2 0.00% 317 2 0.00% 318 2 0.00% 319 1 0.00% 320 0 0.00% 321 1 0.00% 322 2 0.00% 323 1 0.00% 324 0 0.00% 325 1 0.00% 326 0 0.00% 327 0 0.00% 328 3 0.00% 329 0 0.00% 330 0 0.00% 331 1 0.00% 332 3 0.00% 333 0 0.00% 334 1 0.00% 335 1 0.00% 336 0 0.00% 337 3 0.00% 338 0 0.00% 339 2 0.00% 340 0 0.00% 341 1 0.00% 342 3 0.00% 343 0 0.00% 344 1 0.00% 345 0 0.00% 346 0 0.00% 347 0 0.00% 348 1 0.00% 349 0 0.00% 350 1 0.00% 351 0 0.00% 352 0 0.00% 353 0 0.00% 354 0 0.00% 355 3 0.00% 356 0 0.00% 357 0 0.00% 358 0 0.00% 359 0 0.00% 360 2 0.00% 361 0 0.00% 362 1 0.00% 363 1 0.00% 364 0 0.00% 365 1 0.00% 366 2 0.00% 367 2 0.00% 21138562 reads passed initial QC criterion=sequence-density sequence-density=1.61 sequence-density-rank=1 fanout-score=0.00 fanout-score-rank=42 prefix-density=0.00 prefix-fanout=1.0 sequence=ACAGCTATCACACACAGGCAAAACACAGCTGATTCGTGTACTCGATCTCCCCAGCAAGTTAAGGCCACCATGTCGAGCGGCTGCGGCAACTGCGACTGCGCTGACAAGACCCAGTGTGTGAAGAAGGGAAACGGCTACGGCATCGTCATGGTTGACACCGAGAAGAGCCACTTCGAGGTGCAGGAGTCCGCGGCGGAGAACGACGGCAAGTGCAAGTGCGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=54.65 fanout-score-rank=1 prefix-density=0.13 prefix-fanout=4.4 sequence=CATCTCCTCCAGAGATTAAGGGTTTGATAAAGGATAAGAAGACAGCCAAGATCAAGAAGAACTCTGTTGCAGTCACCAAGTTCCTTGATGATT Started job on | Dec 10 00:57:19 Started mapping on | Dec 10 00:57:20 Finished on | Dec 10 00:57:58 Mapping speed, Million of reads per hour | 2002.60 Number of input reads | 21138562 Average input read length | 118 UNIQUE READS: Uniquely mapped reads number | 17856472 Uniquely mapped reads % | 84.47% Average mapped length | 117.43 Number of splices: Total | 6143208 Number of splices: Annotated (sjdb) | 5620872 Number of splices: GT/AG | 5871232 Number of splices: GC/AG | 76495 Number of splices: AT/AC | 4059 Number of splices: Non-canonical | 191422 Mismatch rate per base, % | 0.36% Deletion rate per base | 0.26% Deletion average length | 1.18 Insertion rate per base | 0.16% Insertion average length | 1.16 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 791905 % of reads mapped to multiple loci | 3.75% Number of reads mapped to too many loci | 733970 % of reads mapped to too many loci | 3.47% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 6.74% % of reads unmapped: other | 1.57% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2490185 2490185 2490185 N_multimapping 791905 791905 791905 N_noFeature 644109 768243 17363432 N_ambiguous 422965 61691 1710 UnstrandedReadsAssigned:16789398 PositiveStrandReadsAssigned:17026538 NegativeStrandReadsAssigned:491330 Dataset is classified positive stranded MeadianReadLen=118 20thPercentileLength=86 echo kmer=81 ERR1943001 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR1943001-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 21,138,562 reads, 17,601,560 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,196 rounds 52973 ERR1943001.ke.tsv 35125 ERR1943001.se.tsv 88098 total ==> ERR1943001.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 289.63 27.96 PNS24247 1044 945 0 0 PNS24249 1928 1829 56.0306 2.47532 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 32.3395 1.90458 PNS24243 293 194 0 0 KQK14069 1603 1504 6864.88 368.812 KQK14071 474 375 56.4227 12.1575 ==> ERR1943001.se.tsv <== BRADI_1g14170v3 7015 BRADI_1g53295v3 147 BRADI_1g59795v3 423 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 498 BRADI_1g74790v3 372 BRADI_1g09890v3 24 BRADI_1g77505v3 564 BRADI_1g48960v3 0 ERR1943001 completed mapping pipeline successfully