Starting /dee2/code/volunteer_pipeline.sh ERR3317427 current disk space = 1525383815168 free memory = 1602349200 ERR3317427 SRAfilesize 66f9f33d4b3185a2dac47f41b0599869 ERR3317427.sra ERR3317427.sra file validated ERR3317427 is paired end ERR3317427 is conventional basespace ERR3317427 read1 length is 125 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR3317427_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.20375 33.0 33.0 33.0 33.0 33.0 2 32.25825 33.0 33.0 33.0 33.0 33.0 3 32.329 33.0 33.0 33.0 33.0 33.0 4 32.59 33.0 33.0 33.0 33.0 33.0 5 32.53 33.0 33.0 33.0 33.0 33.0 6 36.0705 37.0 37.0 37.0 33.0 37.0 7 36.33875 37.0 37.0 37.0 37.0 37.0 8 36.336 37.0 37.0 37.0 37.0 37.0 9 36.3865 37.0 37.0 37.0 37.0 37.0 10-11 36.3865 37.0 37.0 37.0 37.0 37.0 12-13 36.381874999999994 37.0 37.0 37.0 37.0 37.0 14-15 36.389 37.0 37.0 37.0 37.0 37.0 16-17 36.418499999999995 37.0 37.0 37.0 37.0 37.0 18-19 36.43025 37.0 37.0 37.0 37.0 37.0 20-21 36.314875 37.0 37.0 37.0 37.0 37.0 22-23 36.31425 37.0 37.0 37.0 37.0 37.0 24-25 36.282875000000004 37.0 37.0 37.0 37.0 37.0 26-27 36.292125 37.0 37.0 37.0 37.0 37.0 28-29 36.213125000000005 37.0 37.0 37.0 37.0 37.0 30-31 36.075 37.0 37.0 37.0 37.0 37.0 32-33 36.166624999999996 37.0 37.0 37.0 37.0 37.0 34-35 36.177375 37.0 37.0 37.0 37.0 37.0 36-37 36.14675 37.0 37.0 37.0 37.0 37.0 38-39 36.042375 37.0 37.0 37.0 37.0 37.0 40-41 36.099999999999994 37.0 37.0 37.0 37.0 37.0 42-43 35.948 37.0 37.0 37.0 37.0 37.0 44-45 36.0105 37.0 37.0 37.0 37.0 37.0 46-47 35.775375 37.0 37.0 37.0 33.0 37.0 48-49 35.765875 37.0 37.0 37.0 33.0 37.0 50-51 35.797125 37.0 37.0 37.0 33.0 37.0 52-53 35.837 37.0 37.0 37.0 33.0 37.0 54-55 35.757125 37.0 37.0 37.0 33.0 37.0 56-57 35.735125 37.0 37.0 37.0 33.0 37.0 58-59 35.774874999999994 37.0 37.0 37.0 33.0 37.0 60-61 35.795625 37.0 37.0 37.0 33.0 37.0 62-63 35.737624999999994 37.0 37.0 37.0 33.0 37.0 64-65 35.766625000000005 37.0 37.0 37.0 33.0 37.0 66-67 35.654624999999996 37.0 37.0 37.0 33.0 37.0 68-69 35.559125 37.0 37.0 37.0 33.0 37.0 70-71 35.637375 37.0 37.0 37.0 33.0 37.0 72-73 35.642375 37.0 37.0 37.0 33.0 37.0 74-75 35.520875000000004 37.0 37.0 37.0 33.0 37.0 76-77 35.4255 37.0 37.0 37.0 33.0 37.0 78-79 35.334875 37.0 37.0 37.0 33.0 37.0 80-81 35.153375 37.0 37.0 37.0 33.0 37.0 82-83 34.890625 37.0 37.0 37.0 33.0 37.0 84-85 34.893375000000006 37.0 37.0 37.0 33.0 37.0 86-87 34.924875 37.0 37.0 37.0 33.0 37.0 88-89 34.814750000000004 37.0 37.0 37.0 33.0 37.0 90-91 34.727125 37.0 37.0 37.0 33.0 37.0 92-93 34.704875 37.0 37.0 37.0 33.0 37.0 94-95 34.668375 37.0 37.0 37.0 33.0 37.0 96-97 34.52275 37.0 37.0 37.0 30.0 37.0 98-99 34.46925 37.0 37.0 37.0 30.0 37.0 100-101 34.443124999999995 37.0 37.0 37.0 27.0 37.0 102-103 34.240625 37.0 37.0 37.0 27.0 37.0 104-105 34.182125 37.0 37.0 37.0 27.0 37.0 106-107 34.159625000000005 37.0 37.0 37.0 27.0 37.0 108-109 34.081500000000005 37.0 37.0 37.0 27.0 37.0 110-111 33.997875 37.0 37.0 37.0 27.0 37.0 112-113 33.937875000000005 37.0 37.0 37.0 27.0 37.0 114-115 33.845625 37.0 37.0 37.0 27.0 37.0 116-117 33.675250000000005 37.0 37.0 37.0 24.5 37.0 118-119 33.4985 37.0 37.0 37.0 22.0 37.0 120-121 33.391999999999996 37.0 37.0 37.0 22.0 37.0 122-123 32.993625 37.0 37.0 37.0 18.0 37.0 124-125 30.8675 37.0 32.0 37.0 8.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 0.0 4 1.0 5 1.0 6 0.0 7 5.0 8 3.0 9 1.0 10 0.0 11 0.0 12 0.0 13 0.0 14 4.0 15 1.0 16 1.0 17 3.0 18 5.0 19 5.0 20 7.0 21 17.0 22 16.0 23 21.0 24 25.0 25 17.0 26 19.0 27 22.0 28 38.0 29 43.0 30 45.0 31 75.0 32 109.0 33 149.0 34 259.0 35 522.0 36 2583.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 22.881799163179917 26.699790794979076 21.338912133891213 29.079497907949794 2 25.25 24.325 21.175 29.25 3 25.874999999999996 24.925 19.575 29.625 4 27.1 23.3 19.45 30.15 5 30.95 22.375 16.55 30.125 6 29.049999999999997 25.0 20.775 25.174999999999997 7 26.650000000000002 22.825 23.75 26.775 8 28.275 25.35 27.625 18.75 9 28.449999999999996 27.6 27.425 16.525000000000002 10-11 29.65 25.937500000000004 24.9375 19.475 12-13 27.6875 24.887500000000003 25.724999999999998 21.7 14-15 25.8625 25.7625 25.912499999999998 22.4625 16-17 26.025 24.1875 26.35 23.4375 18-19 26.387500000000003 25.412499999999998 25.937500000000004 22.2625 20-21 25.074999999999996 25.05 26.0625 23.8125 22-23 25.1 24.075 26.8 24.025 24-25 24.675 25.0 27.1125 23.2125 26-27 25.587500000000002 24.1375 26.474999999999998 23.799999999999997 28-29 26.450000000000003 24.5375 25.6125 23.400000000000002 30-31 25.5125 25.275 25.525 23.6875 32-33 24.8 25.0 26.8375 23.3625 34-35 24.7 25.025 26.025 24.25 36-37 25.687500000000004 25.224999999999998 26.625 22.4625 38-39 24.099999999999998 25.0 26.075 24.825 40-41 25.624999999999996 24.8 25.6125 23.962500000000002 42-43 25.0625 25.124999999999996 26.05 23.7625 44-45 24.8625 25.424999999999997 26.337500000000002 23.375 46-47 26.0 24.4375 25.75 23.8125 48-49 25.0625 24.962500000000002 26.2625 23.7125 50-51 25.087500000000002 23.75 27.575 23.5875 52-53 26.224999999999998 23.6875 26.8625 23.225 54-55 25.7625 25.374999999999996 26.4625 22.400000000000002 56-57 25.587500000000002 24.25 27.200000000000003 22.9625 58-59 25.7875 23.724999999999998 26.5125 23.974999999999998 60-61 24.975 24.45 27.250000000000004 23.325000000000003 62-63 24.6625 24.099999999999998 28.299999999999997 22.9375 64-65 24.9375 24.425 26.2125 24.425 66-67 24.375 24.075 27.35 24.2 68-69 24.95 25.724999999999998 26.6125 22.7125 70-71 25.887500000000003 24.275 25.95 23.8875 72-73 25.25 26.3 25.5125 22.9375 74-75 25.75 25.937500000000004 26.2125 22.1 76-77 25.9625 26.6 25.3125 22.125 78-79 24.637500000000003 26.174999999999997 26.4625 22.725 80-81 24.05 26.275 26.8625 22.8125 82-83 25.362499999999997 25.775 25.624999999999996 23.2375 84-85 24.337500000000002 26.224999999999998 26.1625 23.275000000000002 86-87 24.95 25.2625 26.0125 23.775 88-89 25.45 25.224999999999998 25.3125 24.0125 90-91 24.9375 25.025 27.075 22.9625 92-93 25.112499999999997 25.662499999999998 26.2125 23.0125 94-95 26.4125 24.587500000000002 25.7 23.3 96-97 25.7 24.625 27.05 22.625 98-99 25.2875 25.025 26.174999999999997 23.5125 100-101 25.7125 25.137500000000003 25.587500000000002 23.5625 102-103 25.724999999999998 25.924999999999997 25.575 22.775000000000002 104-105 24.875 25.724999999999998 25.324999999999996 24.075 106-107 25.95 25.9625 24.6125 23.474999999999998 108-109 25.35 26.1 25.525 23.025000000000002 110-111 25.674999999999997 26.2625 25.2625 22.8 112-113 25.3 25.837500000000002 25.624999999999996 23.2375 114-115 24.825 25.900000000000002 25.4375 23.8375 116-117 24.8 26.924999999999997 25.1 23.175 118-119 25.7625 26.224999999999998 25.387500000000003 22.625 120-121 25.224999999999998 25.1875 26.0625 23.525 122-123 24.675 26.200000000000003 25.224999999999998 23.9 124-125 25.7875 26.2125 24.2625 23.7375 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 1.0 21 1.0 22 1.0 23 1.5 24 2.5 25 3.0 26 2.5 27 3.5 28 8.0 29 10.0 30 12.0 31 14.5 32 18.5 33 21.5 34 35.0 35 53.0 36 62.5 37 70.0 38 88.5 39 108.0 40 117.0 41 137.5 42 181.5 43 190.5 44 171.0 45 193.5 46 200.5 47 196.0 48 194.0 49 164.5 50 156.0 51 147.5 52 119.0 53 123.0 54 124.5 55 98.5 56 83.0 57 80.5 58 79.5 59 69.0 60 58.5 61 61.0 62 60.5 63 49.0 64 43.5 65 42.0 66 44.5 67 45.0 68 38.0 69 38.5 70 28.0 71 26.5 72 29.0 73 19.0 74 13.5 75 9.5 76 11.0 77 8.0 78 6.5 79 7.0 80 4.5 81 3.5 82 1.5 83 1.5 84 1.5 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.3999999999999995 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.675 #Duplication Level Percentage of deduplicated Percentage of total 1 97.56917507111456 94.325 2 1.7326092578226013 3.35 3 0.49133695371088704 1.425 4 0.1034393586759762 0.4 5 0.1034393586759762 0.5 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGA 5 0.125 No Hit TATCCCCTGCTTCCCCGCTTCCTCTTCCTCCTCCCCCTCACTCCCCAAGC 5 0.125 No Hit CCACAGCTTAATCCCTATTTTACTCCTAGAAATAGAACACAGCCATATAA 5 0.125 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG 5 0.125 TruSeq Adapter, Index 1 (100% over 49bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.125 0.0 0.0 0.0 0.0 2 0.125 0.0 0.0 0.0 0.0 3 0.175 0.0 0.0 0.0 0.0 4 0.3 0.0 0.0 0.0 0.0 5 0.425 0.0 0.0 0.0 0.0 6 0.55 0.0 0.0 0.0 0.0 7 1.175 0.0 0.0 0.0 0.0 8 1.45 0.0 0.0 0.0 0.0 9 1.5 0.0 0.0 0.0 0.0 10-11 1.525 0.0 0.0 0.0 0.0 12-13 1.525 0.0 0.0 0.0 0.0 14-15 1.525 0.0 0.0 0.0 0.0 16-17 1.55 0.0 0.0 0.0 0.0 18-19 1.55 0.0 0.0 0.0 0.0 20-21 1.575 0.0 0.0 0.0 0.0 22-23 1.6 0.0 0.0 0.0 0.0 24-25 1.6125 0.0 0.0 0.0 0.0 26-27 1.625 0.0 0.0 0.0 0.0 28-29 1.625 0.0 0.0 0.0 0.0 30-31 1.625 0.0 0.0 0.0 0.0 32-33 1.65 0.0 0.0 0.0 0.0 34-35 1.6875 0.0 0.0 0.0 0.0 36-37 1.725 0.0 0.0 0.0 0.0 38-39 1.75 0.0 0.0 0.0 0.0 40-41 1.7625 0.0 0.0 0.0 0.0 42-43 1.8125 0.0 0.0 0.0 0.0 44-45 1.85 0.0 0.0 0.0 0.0 46-47 1.8625 0.0 0.0 0.0 0.0 48-49 1.9 0.0 0.0 0.0 0.0 50-51 1.975 0.0 0.0 0.0 0.0 52-53 2.025 0.0 0.0 0.0 0.0 54-55 2.1125 0.0 0.0 0.0 0.0 56-57 2.1624999999999996 0.0 0.0 0.0 0.0 58-59 2.2 0.0 0.0 0.0 0.0 60-61 2.3 0.0 0.0 0.0 0.0 62-63 2.5125 0.0 0.0 0.0 0.0 64-65 2.6375 0.0 0.0 0.0 0.0 66-67 2.7 0.0 0.0 0.0 0.0 68-69 2.7375 0.0 0.0 0.0 0.0 70-71 2.8625 0.0 0.0 0.0 0.0 72-73 2.9625 0.0 0.0 0.0 0.0 74-75 3.0875000000000004 0.0 0.0 0.0 0.0 76-77 3.2375 0.0 0.0 0.0 0.0 78-79 3.45 0.0 0.0 0.0 0.0 80-81 3.575 0.0 0.0 0.0 0.0 82-83 3.7625 0.0 0.0 0.0 0.0 84-85 4.025 0.0 0.0 0.0 0.0 86-87 4.237500000000001 0.0 0.0 0.0 0.0 88-89 4.5 0.0 0.0 0.0 0.0 90-91 4.925000000000001 0.0 0.0 0.0 0.0 92-93 5.175 0.0 0.0 0.0 0.0 94-95 5.8125 0.0 0.0 0.0 0.0 96-97 6.3375 0.0 0.0 0.0 0.0 98-99 6.775 0.0 0.0 0.0 0.0 100-101 7.2875 0.0 0.0 0.0 0.0 102-103 7.725 0.0 0.0 0.0 0.0 104-105 8.1125 0.0 0.0 0.0 0.0 106-107 8.625 0.0 0.0 0.0 0.0 108-109 9.274999999999999 0.0 0.0 0.0 0.0 110-111 10.075 0.0 0.0 0.0 0.0 112-113 10.587499999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE ERR3317427 read2 length is 125 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR3317427_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.9785 33.0 33.0 33.0 33.0 33.0 2 32.07975 33.0 33.0 33.0 33.0 33.0 3 32.02325 33.0 33.0 33.0 33.0 33.0 4 32.03325 33.0 33.0 33.0 33.0 33.0 5 31.99225 33.0 33.0 33.0 33.0 33.0 6 35.685 37.0 37.0 37.0 37.0 37.0 7 35.6775 37.0 37.0 37.0 37.0 37.0 8 35.7065 37.0 37.0 37.0 37.0 37.0 9 35.73025 37.0 37.0 37.0 37.0 37.0 10-11 35.64775 37.0 37.0 37.0 37.0 37.0 12-13 35.708625 37.0 37.0 37.0 37.0 37.0 14-15 35.62975 37.0 37.0 37.0 37.0 37.0 16-17 35.704375 37.0 37.0 37.0 37.0 37.0 18-19 35.663125 37.0 37.0 37.0 37.0 37.0 20-21 35.581875 37.0 37.0 37.0 37.0 37.0 22-23 35.581875 37.0 37.0 37.0 37.0 37.0 24-25 35.50025 37.0 37.0 37.0 37.0 37.0 26-27 35.543499999999995 37.0 37.0 37.0 37.0 37.0 28-29 35.601 37.0 37.0 37.0 37.0 37.0 30-31 35.500125 37.0 37.0 37.0 37.0 37.0 32-33 35.496625 37.0 37.0 37.0 37.0 37.0 34-35 35.563 37.0 37.0 37.0 37.0 37.0 36-37 35.528125 37.0 37.0 37.0 37.0 37.0 38-39 35.370625000000004 37.0 37.0 37.0 37.0 37.0 40-41 35.363 37.0 37.0 37.0 33.0 37.0 42-43 35.361999999999995 37.0 37.0 37.0 33.0 37.0 44-45 35.219375 37.0 37.0 37.0 33.0 37.0 46-47 35.335375 37.0 37.0 37.0 33.0 37.0 48-49 35.2805 37.0 37.0 37.0 33.0 37.0 50-51 35.32925 37.0 37.0 37.0 37.0 37.0 52-53 35.270375 37.0 37.0 37.0 33.0 37.0 54-55 35.260625000000005 37.0 37.0 37.0 33.0 37.0 56-57 35.2005 37.0 37.0 37.0 33.0 37.0 58-59 35.275 37.0 37.0 37.0 33.0 37.0 60-61 35.3665 37.0 37.0 37.0 35.0 37.0 62-63 35.211749999999995 37.0 37.0 37.0 33.0 37.0 64-65 35.221625 37.0 37.0 37.0 33.0 37.0 66-67 35.234624999999994 37.0 37.0 37.0 33.0 37.0 68-69 35.112375 37.0 37.0 37.0 33.0 37.0 70-71 35.017625 37.0 37.0 37.0 33.0 37.0 72-73 34.82475 37.0 37.0 37.0 33.0 37.0 74-75 34.871875 37.0 37.0 37.0 33.0 37.0 76-77 34.660875000000004 37.0 37.0 37.0 33.0 37.0 78-79 34.64175 37.0 37.0 37.0 33.0 37.0 80-81 34.572874999999996 37.0 37.0 37.0 33.0 37.0 82-83 34.430375 37.0 37.0 37.0 33.0 37.0 84-85 34.457125000000005 37.0 37.0 37.0 33.0 37.0 86-87 34.4495 37.0 37.0 37.0 33.0 37.0 88-89 34.18875 37.0 37.0 37.0 30.0 37.0 90-91 33.9315 37.0 37.0 37.0 27.0 37.0 92-93 33.791875000000005 37.0 37.0 37.0 27.0 37.0 94-95 33.574125 37.0 37.0 37.0 24.5 37.0 96-97 33.272000000000006 37.0 37.0 37.0 20.5 37.0 98-99 33.0925 37.0 37.0 37.0 14.0 37.0 100-101 32.81675 37.0 37.0 37.0 14.0 37.0 102-103 32.42225 37.0 37.0 37.0 8.0 37.0 104-105 32.164500000000004 37.0 37.0 37.0 2.0 37.0 106-107 32.080625 37.0 37.0 37.0 2.0 37.0 108-109 31.474625 37.0 37.0 37.0 2.0 37.0 110-111 31.417375 37.0 37.0 37.0 2.0 37.0 112-113 31.163125 37.0 37.0 37.0 2.0 37.0 114-115 30.851374999999997 37.0 33.0 37.0 2.0 37.0 116-117 30.86925 37.0 33.0 37.0 2.0 37.0 118-119 30.662374999999997 37.0 33.0 37.0 2.0 37.0 120-121 30.575125 37.0 33.0 37.0 2.0 37.0 122-123 30.381875 37.0 33.0 37.0 2.0 37.0 124-125 28.899 37.0 27.5 37.0 2.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56.0 3 5.0 4 8.0 5 5.0 6 4.0 7 4.0 8 6.0 9 0.0 10 5.0 11 1.0 12 2.0 13 3.0 14 2.0 15 5.0 16 3.0 17 6.0 18 4.0 19 4.0 20 13.0 21 22.0 22 43.0 23 16.0 24 13.0 25 18.0 26 77.0 27 62.0 28 71.0 29 92.0 30 80.0 31 63.0 32 100.0 33 115.0 34 135.0 35 333.0 36 2624.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 30.9 12.25 24.675 32.175 2 27.625 11.600000000000001 23.625 37.15 3 23.400000000000002 14.524999999999999 26.575 35.5 4 25.974999999999998 13.825000000000001 25.324999999999996 34.875 5 26.650000000000002 14.799999999999999 22.7 35.85 6 28.425 19.35 26.1 26.125 7 20.0 27.825 35.475 16.7 8 18.475 28.199999999999996 33.15 20.175 9 19.925 29.375 32.125 18.575 10-11 19.537499999999998 30.012499999999996 30.3 20.150000000000002 12-13 20.4125 29.2375 29.3375 21.0125 14-15 20.3375 28.6375 28.825 22.2 16-17 21.15 28.325 26.7625 23.7625 18-19 20.6125 27.950000000000003 27.55 23.8875 20-21 21.375 28.175 26.474999999999998 23.974999999999998 22-23 20.8625 27.400000000000002 26.724999999999998 25.0125 24-25 20.875 28.0875 27.3 23.7375 26-27 21.4 28.7 25.412499999999998 24.4875 28-29 22.05 28.475 24.4875 24.9875 30-31 21.325 29.1125 25.924999999999997 23.6375 32-33 22.125 29.1375 24.4 24.337500000000002 34-35 21.475 27.075 25.474999999999998 25.974999999999998 36-37 21.987499999999997 27.6625 26.187500000000004 24.1625 38-39 22.225 27.450000000000003 25.387500000000003 24.9375 40-41 22.425 26.8625 25.387500000000003 25.324999999999996 42-43 22.375 27.625 25.3 24.7 44-45 23.974999999999998 27.775 24.2875 23.962500000000002 46-47 22.680670167541887 27.344336084021002 24.63115778944736 25.343835958989747 48-49 22.325 28.962500000000002 24.575 24.1375 50-51 23.45 26.575 25.0125 24.962500000000002 52-53 22.375 27.787499999999998 24.1875 25.650000000000002 54-55 22.112499999999997 28.512500000000003 25.887500000000003 23.4875 56-57 22.275 27.9125 25.4875 24.325 58-59 21.925 27.3875 25.0625 25.624999999999996 60-61 20.875 28.375 26.3125 24.4375 62-63 21.462500000000002 28.812500000000004 23.974999999999998 25.75 64-65 22.425 27.5625 24.6625 25.35 66-67 21.587500000000002 29.3875 25.074999999999996 23.95 68-69 23.1125 27.625 23.925 25.337500000000002 70-71 21.7 29.049999999999997 23.75 25.5 72-73 21.4875 28.749999999999996 24.675 25.087500000000002 74-75 22.3875 28.1625 24.8625 24.587500000000002 76-77 23.225 28.025 23.2875 25.4625 78-79 22.3625 28.6375 24.0 25.0 80-81 21.575 28.9875 24.3875 25.05 82-83 22.8625 26.75 24.2375 26.150000000000002 84-85 24.3 27.474999999999998 24.25 23.974999999999998 86-87 24.075 27.775 24.425 23.724999999999998 88-89 23.68222891566265 27.334337349397593 23.406124497991968 25.577309236947794 90-91 24.35023971738582 28.513752207923293 23.31566994700984 23.82033812768105 92-93 23.252279635258358 26.671732522796354 24.46808510638298 25.60790273556231 94-95 23.156549520766774 27.182108626198083 24.61341853035144 25.047923322683708 96-97 24.804110468850354 27.578676942838793 24.213230571612073 23.40398201669878 98-99 24.26119499290231 26.442121564072785 23.00942057039618 26.287262872628723 100-101 24.159061277705344 26.23207301173403 23.95045632333768 25.658409387222946 102-103 24.727224924411725 27.540423294334165 24.096227159195475 23.63612462205863 104-105 23.36163438577872 28.49562218094985 23.87901300079597 24.26373043247546 106-107 24.890598063917253 26.508420633868184 23.166688768067896 25.434292534146664 108-109 23.327037236913114 27.293577981651374 24.6492174851592 24.73016729627631 110-111 24.952994896588773 26.685468708031156 24.08004297609455 24.28149341928552 112-113 24.442025040827435 26.687534022863364 23.707131192161132 25.163309744148066 114-115 25.170905113480995 26.688542521192232 23.98140552365327 24.159146841673504 116-117 25.125423728813562 26.345762711864406 23.53898305084746 24.989830508474576 118-119 24.379823594266814 27.012127894156563 23.91124586549063 24.696802646085995 120-121 23.784006595218464 28.565539983511957 24.36108821104699 23.289365210222588 122-123 25.70093457943925 28.051126992853213 22.553600879604176 23.694337548103352 124-125 25.164203612479476 27.79146141215107 23.864258347016968 23.18007662835249 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 1.0 2 1.5 3 0.5 4 0.5 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 1.0 23 2.0 24 4.5 25 8.0 26 9.0 27 12.0 28 13.5 29 10.5 30 17.0 31 27.0 32 31.0 33 39.5 34 55.5 35 63.0 36 82.0 37 98.0 38 106.5 39 127.5 40 151.5 41 181.5 42 195.5 43 198.5 44 209.5 45 207.0 46 180.0 47 173.0 48 172.5 49 149.5 50 138.5 51 136.5 52 120.5 53 107.0 54 111.5 55 105.0 56 82.5 57 71.5 58 67.0 59 57.0 60 46.5 61 37.5 62 33.0 63 35.5 64 35.5 65 36.0 66 33.0 67 22.5 68 22.0 69 25.0 70 29.5 71 28.0 72 16.0 73 13.5 74 12.5 75 11.5 76 9.0 77 6.5 78 5.5 79 4.0 80 3.5 81 2.0 82 1.5 83 1.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.025 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.4 90-91 0.9249999999999999 92-93 1.3 94-95 2.1875 96-97 2.6875 98-99 3.1375 100-101 4.125 102-103 4.9125000000000005 104-105 5.775 106-107 5.7375 108-109 7.35 110-111 6.925000000000001 112-113 8.15 114-115 8.575000000000001 116-117 7.8125 118-119 9.3 120-121 9.025 122-123 9.049999999999999 124-125 8.649999999999999 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.675 #Duplication Level Percentage of deduplicated Percentage of total 1 98.56667519836192 96.275 2 1.0749936012285641 2.1 3 0.2047606859482979 0.6 4 0.02559508574353724 0.1 5 0.0 0.0 6 0.02559508574353724 0.15 7 0.07678525723061172 0.525 8 0.0 0.0 9 0.0 0.0 >10 0.02559508574353724 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCACCAAATTGTAATACAGAATCATCCCCAAAGATTTCGGTCAGAGCTGG 10 0.25 No Hit TCTTCATATTTAGTTTTATCTATTAATCGATAGTATCTACCGGCGCGAAC 7 0.17500000000000002 No Hit TGGGGATGAAAAATAGAAAACTCAAAGCAATGTCAGAGTTGACAAAACTG 7 0.17500000000000002 No Hit CCACCGAATTGTAATACAGAATCATCCCCAAAGATTTCGGTCAGAGCTGG 7 0.17500000000000002 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 6 0.15 Illumina Single End PCR Primer 1 (100% over 50bp) >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.15 0.0 0.0 0.0 0.0 2 0.15 0.0 0.0 0.0 0.0 3 0.2 0.0 0.0 0.0 0.0 4 0.325 0.0 0.0 0.0 0.0 5 0.475 0.0 0.0 0.0 0.0 6 0.6 0.0 0.0 0.0 0.0 7 1.25 0.0 0.0 0.0 0.0 8 1.55 0.0 0.0 0.0 0.0 9 1.6 0.0 0.0 0.0 0.0 10-11 1.625 0.0 0.0 0.0 0.0 12-13 1.625 0.0 0.0 0.0 0.0 14-15 1.625 0.0 0.0 0.0 0.0 16-17 1.65 0.0 0.0 0.0 0.0 18-19 1.65 0.0 0.0 0.0 0.0 20-21 1.675 0.0 0.0 0.0 0.0 22-23 1.7 0.0 0.0 0.0 0.0 24-25 1.7125 0.0 0.0 0.0 0.0 26-27 1.725 0.0 0.0 0.0 0.0 28-29 1.725 0.0 0.0 0.0 0.0 30-31 1.725 0.0 0.0 0.0 0.0 32-33 1.7375 0.0 0.0 0.0 0.0 34-35 1.7625 0.0 0.0 0.0 0.0 36-37 1.8 0.0 0.0 0.0 0.0 38-39 1.825 0.0 0.0 0.0 0.0 40-41 1.8375 0.0 0.0 0.0 0.0 42-43 1.8875 0.0 0.0 0.0 0.0 44-45 1.925 0.0 0.0 0.0 0.0 46-47 1.925 0.0 0.0 0.0 0.0 48-49 1.9625 0.0 0.0 0.0 0.0 50-51 2.025 0.0 0.0 0.0 0.0 52-53 2.075 0.0 0.0 0.0 0.0 54-55 2.1624999999999996 0.0 0.0 0.0 0.0 56-57 2.1875 0.0 0.0 0.0 0.0 58-59 2.2125000000000004 0.0 0.0 0.0 0.0 60-61 2.3 0.0 0.0 0.0 0.0 62-63 2.4875 0.0 0.0 0.0 0.0 64-65 2.5875000000000004 0.0 0.0 0.0 0.0 66-67 2.65 0.0 0.0 0.0 0.0 68-69 2.6875 0.0 0.0 0.0 0.0 70-71 2.8125 0.0 0.0 0.0 0.0 72-73 2.9125 0.0 0.0 0.0 0.0 74-75 3.0374999999999996 0.0 0.0 0.0 0.0 76-77 3.1625 0.0 0.0 0.0 0.0 78-79 3.3625 0.0 0.0 0.0 0.0 80-81 3.525 0.0 0.0 0.0 0.0 82-83 3.7375 0.0 0.0 0.0 0.0 84-85 4.0125 0.0 0.0 0.0 0.0 86-87 4.25 0.0 0.0 0.0 0.0 88-89 4.525 0.0 0.0 0.0 0.0 90-91 4.875 0.0 0.0 0.0 0.0 92-93 5.0625 0.0 0.0 0.0 0.0 94-95 5.6375 0.0 0.0 0.0 0.0 96-97 6.074999999999999 0.0 0.0 0.0 0.0 98-99 6.4875 0.0 0.0 0.0 0.0 100-101 6.987500000000001 0.0 0.0 0.0 0.0 102-103 7.4125 0.0 0.0 0.0 0.0 104-105 7.7 0.0 0.0 0.0 0.0 106-107 8.1125 0.0 0.0 0.0 0.0 108-109 8.7 0.0 0.0 0.0 0.0 110-111 9.4375 0.0 0.0 0.0 0.0 112-113 9.912500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1787100 spots for ERR3317427.sra Written 1787100 spots for ERR3317427.sra Read 1787100 spots for ERR3317427.sra Written 1787100 spots for ERR3317427.sra Read 1787100 spots for ERR3317427.sra Written 1787100 spots for ERR3317427.sra Read 1787100 spots for ERR3317427.sra Written 1787100 spots for ERR3317427.sra Read 1787100 spots for ERR3317427.sra Written 1787100 spots for ERR3317427.sra Read 1787100 spots for ERR3317427.sra Written 1787100 spots for ERR3317427.sra Read 1787100 spots for ERR3317427.sra Written 1787100 spots for ERR3317427.sra Read 1787100 spots for ERR3317427.sra Written 1787100 spots for ERR3317427.sra Read 1787100 spots for ERR3317427.sra Written 1787100 spots for ERR3317427.sra Read 1787100 spots for ERR3317427.sra Written 1787100 spots for ERR3317427.sra Read 1787100 spots for ERR3317427.sra Written 1787100 spots for ERR3317427.sra Read 1787100 spots for ERR3317427.sra Written 1787100 spots for ERR3317427.sra Read 1787106 spots for ERR3317427.sra Written 1787106 spots for ERR3317427.sra Read 1787100 spots for ERR3317427.sra Written 1787100 spots for ERR3317427.sra Read 1787100 spots for ERR3317427.sra Written 1787100 spots for ERR3317427.sra Read 1787100 spots for ERR3317427.sra Written 1787100 spots for ERR3317427.sra Read 1787100 spots for ERR3317427.sra Written 1787100 spots for ERR3317427.sra Read 1787100 spots for ERR3317427.sra Written 1787100 spots for ERR3317427.sra Read 1787100 spots for ERR3317427.sra Written 1787100 spots for ERR3317427.sra Read 1787100 spots for ERR3317427.sra Written 1787100 spots for ERR3317427.sra SRR ids: ['ERR3317427.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_zodw4ddk ERR3317427.sra spots: 35742006 blocks: [[1, 1787100], [1787101, 3574200], [3574201, 5361300], [5361301, 7148400], [7148401, 8935500], [8935501, 10722600], [10722601, 12509700], [12509701, 14296800], [14296801, 16083900], [16083901, 17871000], [17871001, 19658100], [19658101, 21445200], [21445201, 23232300], [23232301, 25019400], [25019401, 26806500], [26806501, 28593600], [28593601, 30380700], [30380701, 32167800], [32167801, 33954900], [33954901, 35742006]] ERR3317427 file size 10275068 ERR3317427 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR3317427 ERR3317427_1.fastq ERR3317427_2.fastq Input file: ERR3317427_1.fastq Paired file: ERR3317427_2.fastq trimmed: ERR3317427-trimmed-pair1.fastq, ERR3317427-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Dec 10 09:01:03 2024 >> started Tue Dec 10 09:01:38 2024 >> done (35.073s) 35742006 read pairs processed; of these: 767622 ( 2.15%) short read pairs filtered out after trimming by size control 438817 ( 1.23%) empty read pairs filtered out after trimming by size control 34535567 (96.62%) read pairs available; of these: 12466223 (36.10%) trimmed read pairs available after processing 22069344 (63.90%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 464 0.00% 19 1671 0.00% 20 1820 0.01% 21 2970 0.01% 22 1129 0.00% 23 1162 0.00% 24 794 0.00% 25 913 0.00% 26 1079 0.00% 27 1321 0.00% 28 1218 0.00% 29 1369 0.00% 30 1367 0.00% 31 2228 0.01% 32 1859 0.01% 33 1984 0.01% 34 2924 0.01% 35 3849 0.01% 36 2972 0.01% 37 2793 0.01% 38 3478 0.01% 39 3881 0.01% 40 4266 0.01% 41 4454 0.01% 42 5022 0.01% 43 5480 0.02% 44 5940 0.02% 45 6135 0.02% 46 6300 0.02% 47 7687 0.02% 48 8132 0.02% 49 8673 0.03% 50 9482 0.03% 51 10196 0.03% 52 10251 0.03% 53 11492 0.03% 54 12493 0.04% 55 13840 0.04% 56 13952 0.04% 57 14784 0.04% 58 16176 0.05% 59 17061 0.05% 60 22018 0.06% 61 20099 0.06% 62 21029 0.06% 63 22077 0.06% 64 25479 0.07% 65 24918 0.07% 66 27749 0.08% 67 28020 0.08% 68 30246 0.09% 69 32130 0.09% 70 35011 0.10% 71 43228 0.13% 72 47922 0.14% 73 52814 0.15% 74 52929 0.15% 75 53131 0.15% 76 55858 0.16% 77 56972 0.16% 78 59163 0.17% 79 60043 0.17% 80 61313 0.18% 81 61919 0.18% 82 64287 0.19% 83 67424 0.20% 84 69240 0.20% 85 76060 0.22% 86 80415 0.23% 87 82276 0.24% 88 81130 0.23% 89 92362 0.27% 90 84077 0.24% 91 83174 0.24% 92 86594 0.25% 93 86493 0.25% 94 91633 0.27% 95 93380 0.27% 96 99203 0.29% 97 100508 0.29% 98 99683 0.29% 99 103862 0.30% 100 104604 0.30% 101 111653 0.32% 102 113077 0.33% 103 116947 0.34% 104 118976 0.34% 105 125813 0.36% 106 126861 0.37% 107 133327 0.39% 108 137228 0.40% 109 157435 0.46% 110 148731 0.43% 111 153309 0.44% 112 164411 0.48% 113 166787 0.48% 114 174780 0.51% 115 183382 0.53% 116 196556 0.57% 117 211349 0.61% 118 229055 0.66% 119 261810 0.76% 120 306689 0.89% 121 387165 1.12% 122 539622 1.56% 123 980141 2.84% 124 4445585 12.87% 125 22069344 63.90% 34535567 reads passed initial QC criterion=sequence-density sequence-density=0.34 sequence-density-rank=1 fanout-score=5.31 fanout-score-rank=14 prefix-density=0.43 prefix-fanout=4.2 sequence=AAGGAGCTGGAGGAGGTCAAGAAGGAGTACCCGGACGCCTA criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=174.33 fanout-score-rank=1 prefix-density=0.17 prefix-fanout=11.0 sequence=AAGCTCAAGCATTTACTTTTCTAGTTAGAGACCAGCGTCTTGGAGCTAATGTGGGATCTGCTCAAGGACCCACAGGTTTAGGTAAATATCTAATGCGTTCCCCAACGGGAGAGGTTATCTTTGGAGGGGAAACTATGCGTTTTTGGGACCTCCGTGCTCCATGGTTAGAACCTCTAAGGGGGCCCAACGGTTTGGACTTGAGTAGGTTGAAAAAAGACATACAACCTTGGCAAGAACGACGTTCAGCGGAATATATGACCCATGCTCCTTTAGGCTCTTTAAATTCCGTGGGTGGCGTAGCTACCGAGATCAATGCAGTTAATTATGTCTCTCCTAGAAGTTGGTTATCGACTTCTCATTTTGTTCTAGGATTCTTCTTTTTTGTGGGCCATTTGTGGCATGCAGGAAGAGCCCGAGCTGCTGCAGCAGGTTTTGAAAAGGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAACCCTCTTAACTAAGATTTTCTTATTTATACCTG criterion=sequence-density sequence-density=0.77 sequence-density-rank=1 fanout-score=28.96 fanout-score-rank=6 prefix-density=0.75 prefix-fanout=29.0 sequence=GGTGTGCTCTTCCGATCT criterion=fanout-score sequence-density=0.02 sequence-density-rank=26 fanout-score=209.95 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=15.0 sequence=CTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCGACGTCGGCGGCGGCGAACCGCCCCCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATATACTCGTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCGTCGCCTAAACGGCGATAGTCCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCATAGAATCAA ERR3317427 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 10 09:02:26 Started mapping on | Dec 10 09:02:27 Finished on | Dec 10 09:03:54 Mapping speed, Million of reads per hour | 1429.06 Number of input reads | 34535567 Average input read length | 240 UNIQUE READS: Uniquely mapped reads number | 30099132 Uniquely mapped reads % | 87.15% Average mapped length | 239.02 Number of splices: Total | 18643058 Number of splices: Annotated (sjdb) | 17506292 Number of splices: GT/AG | 18350086 Number of splices: GC/AG | 256762 Number of splices: AT/AC | 15633 Number of splices: Non-canonical | 20577 Mismatch rate per base, % | 0.33% Deletion rate per base | 0.00% Deletion average length | 1.23 Insertion rate per base | 0.00% Insertion average length | 1.24 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 3174093 % of reads mapped to multiple loci | 9.19% Number of reads mapped to too many loci | 179691 % of reads mapped to too many loci | 0.52% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 0.57% % of reads unmapped: other | 2.56% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1307815 1307815 1307815 N_multimapping 3174093 3174093 3174093 N_noFeature 1444421 2932869 27905626 N_ambiguous 1160152 537861 37296 UnstrandedReadsAssigned:27494559 PositiveStrandReadsAssigned:26628402 NegativeStrandReadsAssigned:2156210 Dataset is classified positive stranded MeadianReadLen=125 20thPercentileLength=123 echo kmer=119 ERR3317427 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR3317427-trimmed-pair1.fastq ERR3317427-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 34,535,567 reads, 29,485,247 reads pseudoaligned [quant] estimated average fragment length: 216.988 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,181 rounds 52973 ERR3317427.ke.tsv 35125 ERR3317427.se.tsv 88098 total ==> ERR3317427.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 720.364 9.90636e-06 6.63582e-07 PNS24247 1044 828.012 62.5022 3.64243 PNS24249 1928 1712.01 201.106 5.66828 PNS24246 1044 828.012 62.5022 3.64243 PNS24248 1044 828.012 62.5022 3.64243 PNS24244 1471 1255.01 411.387 15.8174 PNS24243 293 118.238 2 0.816218 KQK14069 1603 1387.01 20574 715.766 KQK14071 474 270.331 60.7982 10.8524 ==> ERR3317427.se.tsv <== BRADI_1g14170v3 22395 BRADI_1g53295v3 376 BRADI_1g59795v3 667 BRADI_1g07683v3 0 BRADI_1g00485v3 47 BRADI_1g20270v3 1797 BRADI_1g74790v3 114 BRADI_1g09890v3 0 BRADI_1g77505v3 433 BRADI_1g48960v3 0 ERR3317427 completed mapping pipeline successfully