Starting /dee2/code/volunteer_pipeline.sh ERR3317431 current disk space = 1525359124480 free memory = 1602324192 ERR3317431 SRAfilesize d52376a5028fc02273c1ee12f89f4d62 ERR3317431.sra ERR3317431.sra file validated ERR3317431 is paired end ERR3317431 is conventional basespace ERR3317431 read1 length is 125 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR3317431_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.98425 33.0 33.0 33.0 33.0 33.0 2 32.127 33.0 33.0 33.0 33.0 33.0 3 31.6945 33.0 33.0 33.0 27.0 33.0 4 31.91675 33.0 33.0 33.0 33.0 33.0 5 32.082 33.0 33.0 33.0 33.0 33.0 6 34.887 37.0 37.0 37.0 27.0 37.0 7 35.389 37.0 37.0 37.0 33.0 37.0 8 35.585 37.0 37.0 37.0 33.0 37.0 9 35.5815 37.0 37.0 37.0 33.0 37.0 10-11 35.64475 37.0 37.0 37.0 33.0 37.0 12-13 35.787625 37.0 37.0 37.0 33.0 37.0 14-15 35.70075 37.0 37.0 37.0 33.0 37.0 16-17 35.810500000000005 37.0 37.0 37.0 33.0 37.0 18-19 35.6105 37.0 37.0 37.0 33.0 37.0 20-21 35.733125 37.0 37.0 37.0 33.0 37.0 22-23 35.723749999999995 37.0 37.0 37.0 33.0 37.0 24-25 35.750375000000005 37.0 37.0 37.0 33.0 37.0 26-27 35.694374999999994 37.0 37.0 37.0 33.0 37.0 28-29 35.53275 37.0 37.0 37.0 33.0 37.0 30-31 35.612375 37.0 37.0 37.0 33.0 37.0 32-33 35.66525 37.0 37.0 37.0 33.0 37.0 34-35 35.488125 37.0 37.0 37.0 33.0 37.0 36-37 35.439750000000004 37.0 37.0 37.0 33.0 37.0 38-39 35.295375 37.0 37.0 37.0 33.0 37.0 40-41 35.2945 37.0 37.0 37.0 33.0 37.0 42-43 35.4405 37.0 37.0 37.0 33.0 37.0 44-45 35.301375 37.0 37.0 37.0 33.0 37.0 46-47 35.106375 37.0 37.0 37.0 33.0 37.0 48-49 35.167249999999996 37.0 37.0 37.0 33.0 37.0 50-51 35.143125 37.0 37.0 37.0 33.0 37.0 52-53 35.04025 37.0 37.0 37.0 33.0 37.0 54-55 34.924375 37.0 37.0 37.0 30.0 37.0 56-57 35.09975 37.0 37.0 37.0 33.0 37.0 58-59 34.810249999999996 37.0 37.0 37.0 30.0 37.0 60-61 34.948625 37.0 37.0 37.0 30.0 37.0 62-63 34.91275 37.0 37.0 37.0 30.0 37.0 64-65 34.91075 37.0 37.0 37.0 27.0 37.0 66-67 34.757625000000004 37.0 37.0 37.0 27.0 37.0 68-69 34.6085 37.0 37.0 37.0 27.0 37.0 70-71 34.7325 37.0 37.0 37.0 27.0 37.0 72-73 34.628375 37.0 37.0 37.0 27.0 37.0 74-75 34.69225 37.0 37.0 37.0 27.0 37.0 76-77 34.62375 37.0 37.0 37.0 27.0 37.0 78-79 34.460125000000005 37.0 37.0 37.0 27.0 37.0 80-81 34.34525 37.0 37.0 37.0 27.0 37.0 82-83 34.27325 37.0 37.0 37.0 27.0 37.0 84-85 34.140875 37.0 37.0 37.0 27.0 37.0 86-87 34.057875 37.0 37.0 37.0 27.0 37.0 88-89 33.92425 37.0 37.0 37.0 27.0 37.0 90-91 33.8855 37.0 37.0 37.0 27.0 37.0 92-93 33.9585 37.0 37.0 37.0 27.0 37.0 94-95 33.847375 37.0 37.0 37.0 27.0 37.0 96-97 34.021125 37.0 37.0 37.0 27.0 37.0 98-99 33.722375 37.0 37.0 37.0 27.0 37.0 100-101 33.691125 37.0 37.0 37.0 27.0 37.0 102-103 33.49675 37.0 35.0 37.0 24.5 37.0 104-105 33.54375 37.0 37.0 37.0 24.5 37.0 106-107 33.460125 37.0 37.0 37.0 22.0 37.0 108-109 33.212500000000006 37.0 33.0 37.0 22.0 37.0 110-111 33.301625 37.0 35.0 37.0 22.0 37.0 112-113 32.875375 37.0 33.0 37.0 18.0 37.0 114-115 32.862 37.0 33.0 37.0 14.0 37.0 116-117 32.7225 37.0 33.0 37.0 14.0 37.0 118-119 32.4595 37.0 33.0 37.0 14.0 37.0 120-121 31.900999999999996 37.0 33.0 37.0 14.0 37.0 122-123 31.491875 37.0 33.0 37.0 8.0 37.0 124-125 29.53125 37.0 30.0 37.0 2.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 1.0 5 1.0 6 1.0 7 1.0 8 7.0 9 1.0 10 2.0 11 1.0 12 3.0 13 4.0 14 5.0 15 4.0 16 8.0 17 4.0 18 9.0 19 8.0 20 11.0 21 12.0 22 18.0 23 21.0 24 19.0 25 38.0 26 33.0 27 51.0 28 68.0 29 83.0 30 95.0 31 135.0 32 194.0 33 208.0 34 324.0 35 579.0 36 2049.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 21.575 28.175 21.9 28.349999999999998 2 25.8 26.35 20.424999999999997 27.425 3 26.514199547625033 26.08695652173913 20.13068610203569 27.26815782860015 4 26.488244122061033 26.21310655327664 19.484742371185593 27.813906953476735 5 29.425 25.5 17.625 27.450000000000003 6 27.925 29.975 17.875 24.224999999999998 7 26.025 27.275 20.825 25.874999999999996 8 30.049999999999997 27.1 25.0 17.849999999999998 9 29.9 29.875 23.200000000000003 17.025000000000002 10-11 30.2375 28.625 23.3375 17.8 12-13 28.262500000000003 27.975 24.1875 19.575 14-15 25.5125 28.8375 25.4 20.25 16-17 25.174999999999997 29.7375 25.275 19.8125 18-19 22.95 30.6875 25.8625 20.5 20-21 23.1125 31.1 25.4875 20.3 22-23 23.925 30.2125 25.05 20.8125 24-25 22.2125 30.2875 26.650000000000002 20.849999999999998 26-27 23.45 29.2875 26.237500000000004 21.025 28-29 24.4375 29.825000000000003 24.3625 21.375 30-31 24.6125 30.112499999999997 25.337500000000002 19.9375 32-33 22.15 30.325000000000003 26.7625 20.7625 34-35 22.8875 30.662499999999998 26.25 20.200000000000003 36-37 22.900000000000002 29.299999999999997 26.5 21.3 38-39 23.400000000000002 29.9375 25.912499999999998 20.75 40-41 22.9375 29.7375 26.35 20.974999999999998 42-43 23.8125 29.512500000000003 26.424999999999997 20.25 44-45 24.75 29.725 25.650000000000002 19.875 46-47 24.975 28.287499999999998 25.324999999999996 21.4125 48-49 24.275 29.975 24.525 21.224999999999998 50-51 23.799999999999997 29.462500000000002 25.874999999999996 20.8625 52-53 23.3375 30.7625 24.6625 21.2375 54-55 22.9875 30.3 26.775 19.9375 56-57 22.7 30.612499999999997 26.174999999999997 20.5125 58-59 22.8 30.3875 25.900000000000002 20.9125 60-61 22.7375 30.7125 26.700000000000003 19.85 62-63 22.675 30.175 25.7 21.45 64-65 22.787499999999998 30.349999999999998 26.375 20.4875 66-67 23.549999999999997 30.2875 25.174999999999997 20.9875 68-69 22.375 30.9625 25.575 21.087500000000002 70-71 23.3875 32.0125 23.9375 20.6625 72-73 22.0625 32.05 25.55 20.3375 74-75 23.3 31.3 24.675 20.724999999999998 76-77 22.1875 32.2875 24.5625 20.962500000000002 78-79 22.95 31.9875 23.849999999999998 21.212500000000002 80-81 22.25 31.612499999999997 25.2 20.9375 82-83 23.8625 30.837500000000002 24.4125 20.8875 84-85 22.7375 30.1875 26.35 20.724999999999998 86-87 23.9125 31.362499999999997 24.075 20.65 88-89 22.725 32.550000000000004 23.974999999999998 20.75 90-91 24.125 31.7875 23.1625 20.925 92-93 22.15 31.324999999999996 24.975 21.55 94-95 22.662499999999998 32.1625 23.7625 21.4125 96-97 22.9875 31.0625 24.762500000000003 21.1875 98-99 22.900000000000002 31.25 23.799999999999997 22.05 100-101 22.275 31.125000000000004 24.337500000000002 22.2625 102-103 23.1375 32.45 23.4125 21.0 104-105 21.712500000000002 31.825 23.1875 23.275000000000002 106-107 22.9375 31.624999999999996 23.4375 22.0 108-109 23.2375 31.7 23.400000000000002 21.6625 110-111 22.537499999999998 32.1125 23.325000000000003 22.025 112-113 23.125 31.65 22.9625 22.2625 114-115 21.975 30.837500000000002 24.125 23.0625 116-117 23.275000000000002 31.3125 22.2 23.2125 118-119 22.2625 32.5 23.1 22.1375 120-121 22.237499999999997 31.624999999999996 22.5125 23.625 122-123 22.037499999999998 31.4875 23.1625 23.3125 124-125 22.425 30.225 24.0375 23.3125 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 1.5 2 1.5 3 0.5 4 0.0 5 0.0 6 0.5 7 0.5 8 0.5 9 0.5 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.5 16 0.5 17 0.0 18 1.0 19 2.0 20 2.0 21 3.0 22 5.5 23 7.5 24 11.5 25 19.5 26 28.0 27 33.5 28 41.0 29 52.0 30 61.0 31 64.5 32 69.0 33 68.0 34 86.0 35 105.0 36 107.0 37 109.0 38 113.0 39 125.0 40 129.5 41 157.5 42 181.5 43 176.5 44 180.5 45 180.5 46 187.5 47 172.0 48 144.5 49 133.5 50 133.0 51 131.0 52 111.0 53 98.5 54 87.0 55 70.0 56 56.5 57 54.0 58 50.0 59 52.0 60 46.0 61 33.0 62 33.5 63 33.5 64 26.5 65 25.5 66 25.0 67 25.5 68 21.0 69 16.0 70 18.0 71 13.5 72 10.5 73 13.0 74 13.0 75 8.5 76 6.5 77 5.5 78 4.5 79 3.0 80 2.5 81 3.0 82 2.0 83 1.5 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.525 4 0.05 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.4 #Duplication Level Percentage of deduplicated Percentage of total 1 97.1627408993576 90.75 2 1.7665952890792294 3.3000000000000003 3 0.4550321199143469 1.275 4 0.1873661670235546 0.7000000000000001 5 0.10706638115631692 0.5 6 0.05353319057815846 0.3 7 0.05353319057815846 0.35000000000000003 8 0.05353319057815846 0.4 9 0.02676659528907923 0.22499999999999998 >10 0.13383297644539613 2.1999999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGG 28 0.7000000000000001 No Hit TGCAAATGGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGAT 23 0.575 No Hit GAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTCGAGTTCGC 16 0.4 No Hit CCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTCGAGTT 11 0.27499999999999997 No Hit CGTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGC 10 0.25 No Hit AGTAGGCGAAATTTAGATCAAAGAGTGAATTAGTAGATTGTGTTTCACGT 9 0.22499999999999998 No Hit CAGTAGAAGGTTCCCTCGGAACAATTATTATATATTTCAAGTTATTTCGG 8 0.2 No Hit TGTGAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTAT 8 0.2 No Hit ACTTAACTGGGGGATTCACCGCAAATACTACTTTGGCTCATTATTGCCGC 7 0.17500000000000002 No Hit TGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTG 7 0.17500000000000002 No Hit GCAAATGGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATC 6 0.15 No Hit GCAGCTTGTGAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGA 6 0.15 No Hit GCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGT 5 0.125 No Hit ACACGCCGGAGGTATGGTCCGAATCTGGCGACCGCTACGCCGGTGGGACG 5 0.125 No Hit AGCTTGTGAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATA 5 0.125 No Hit TAAACCCATTGATAAAAAGAAAAAAAGAGGATAAAAGTTAGGGAATAAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.075 0.0 0.0 0.0 0.0 2 0.075 0.0 0.0 0.0 0.0 3 0.1 0.0 0.0 0.0 0.0 4 0.125 0.0 0.0 0.0 0.0 5 0.175 0.0 0.0 0.0 0.0 6 0.25 0.0 0.0 0.0 0.0 7 0.45 0.0 0.0 0.0 0.0 8 0.5 0.0 0.0 0.0 0.0 9 0.575 0.0 0.0 0.0 0.0 10-11 0.6 0.0 0.0 0.0 0.0 12-13 0.6 0.0 0.0 0.0 0.0 14-15 0.6 0.0 0.0 0.0 0.0 16-17 0.6 0.0 0.0 0.0 0.0 18-19 0.65 0.0 0.0 0.0 0.0 20-21 0.65 0.0 0.0 0.0 0.0 22-23 0.7125 0.0 0.0 0.0 0.0 24-25 0.9 0.0 0.0 0.0 0.0 26-27 0.925 0.0 0.0 0.0 0.0 28-29 0.9624999999999999 0.0 0.0 0.0 0.0 30-31 0.975 0.0 0.0 0.0 0.0 32-33 1.0125 0.0 0.0 0.0 0.0 34-35 1.0499999999999998 0.0 0.0 0.0 0.0 36-37 1.1125 0.0 0.0 0.0 0.0 38-39 1.3375 0.0 0.0 0.0 0.0 40-41 1.5375 0.0 0.0 0.0 0.0 42-43 1.6625 0.0 0.0 0.0 0.0 44-45 1.8250000000000002 0.0 0.0 0.0 0.0 46-47 1.9 0.0 0.0 0.0 0.0 48-49 2.0125 0.0 0.0 0.0 0.0 50-51 2.2249999999999996 0.0 0.0 0.0 0.0 52-53 2.3625 0.0 0.0 0.0 0.0 54-55 2.5875 0.0 0.0 0.0 0.0 56-57 2.6875 0.0 0.0 0.0 0.0 58-59 2.875 0.0 0.0 0.0 0.0 60-61 3.0625 0.0 0.0 0.0 0.0 62-63 3.25 0.0 0.0 0.0 0.0 64-65 3.4375 0.0 0.0 0.0 0.0 66-67 3.7249999999999996 0.0 0.0 0.0 0.0 68-69 3.9875 0.0 0.0 0.0 0.0 70-71 4.4625 0.0 0.0 0.0 0.0 72-73 4.875 0.0 0.0 0.0 0.0 74-75 5.275 0.0 0.0 0.0 0.0 76-77 5.75 0.0 0.0 0.0 0.0 78-79 6.4875 0.0 0.0 0.0 0.0 80-81 7.4 0.0 0.0 0.0 0.0 82-83 8.225 0.0 0.0 0.0 0.0 84-85 8.8375 0.0 0.0 0.0 0.0 86-87 9.7875 0.0 0.0 0.0 0.0 88-89 10.65 0.0 0.0 0.0 0.0 90-91 11.75 0.0 0.0 0.0 0.0 92-93 12.575 0.0 0.0 0.0 0.0 94-95 13.45 0.0 0.0 0.0 0.0 96-97 14.1875 0.0 0.0 0.0 0.0 98-99 14.875 0.0 0.0 0.0 0.0 100-101 15.5625 0.0 0.0 0.0 0.0 102-103 16.4375 0.0 0.0 0.0 0.0 104-105 17.1375 0.0 0.0 0.0 0.0 106-107 18.0625 0.0 0.0 0.0 0.0 108-109 19.35 0.0 0.0 0.0 0.0 110-111 20.5125 0.0 0.0 0.0 0.0 112-113 21.65 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE ERR3317431 read2 length is 125 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR3317431_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.707 33.0 33.0 33.0 33.0 33.0 2 31.8145 33.0 33.0 33.0 33.0 33.0 3 31.7645 33.0 33.0 33.0 33.0 33.0 4 31.7395 33.0 33.0 33.0 33.0 33.0 5 31.79475 33.0 33.0 33.0 33.0 33.0 6 35.23625 37.0 37.0 37.0 33.0 37.0 7 35.06375 37.0 37.0 37.0 33.0 37.0 8 35.20725 37.0 37.0 37.0 33.0 37.0 9 35.22175 37.0 37.0 37.0 33.0 37.0 10-11 35.185 37.0 37.0 37.0 33.0 37.0 12-13 35.175124999999994 37.0 37.0 37.0 33.0 37.0 14-15 35.10724999999999 37.0 37.0 37.0 33.0 37.0 16-17 35.001125 37.0 37.0 37.0 33.0 37.0 18-19 34.911874999999995 37.0 37.0 37.0 33.0 37.0 20-21 34.90025 37.0 37.0 37.0 33.0 37.0 22-23 34.849875 37.0 37.0 37.0 33.0 37.0 24-25 34.8185 37.0 37.0 37.0 30.0 37.0 26-27 34.7365 37.0 37.0 37.0 27.0 37.0 28-29 34.786125 37.0 37.0 37.0 30.0 37.0 30-31 34.712375 37.0 37.0 37.0 27.0 37.0 32-33 34.694500000000005 37.0 37.0 37.0 27.0 37.0 34-35 34.671375 37.0 37.0 37.0 27.0 37.0 36-37 34.682874999999996 37.0 37.0 37.0 30.0 37.0 38-39 34.51075 37.0 37.0 37.0 27.0 37.0 40-41 34.55500000000001 37.0 37.0 37.0 27.0 37.0 42-43 34.472 37.0 37.0 37.0 27.0 37.0 44-45 34.538375 37.0 37.0 37.0 27.0 37.0 46-47 34.416250000000005 37.0 37.0 37.0 27.0 37.0 48-49 34.361999999999995 37.0 37.0 37.0 27.0 37.0 50-51 34.241125 37.0 37.0 37.0 27.0 37.0 52-53 34.186499999999995 37.0 37.0 37.0 27.0 37.0 54-55 34.156 37.0 37.0 37.0 27.0 37.0 56-57 34.22425 37.0 37.0 37.0 27.0 37.0 58-59 34.106 37.0 37.0 37.0 27.0 37.0 60-61 33.987625 37.0 37.0 37.0 27.0 37.0 62-63 33.93962500000001 37.0 37.0 37.0 27.0 37.0 64-65 33.879875 37.0 37.0 37.0 27.0 37.0 66-67 33.8395 37.0 37.0 37.0 27.0 37.0 68-69 33.714124999999996 37.0 37.0 37.0 27.0 37.0 70-71 33.67475 37.0 37.0 37.0 27.0 37.0 72-73 33.672875 37.0 37.0 37.0 27.0 37.0 74-75 33.495625000000004 37.0 37.0 37.0 22.0 37.0 76-77 33.52875 37.0 37.0 37.0 27.0 37.0 78-79 33.414125 37.0 37.0 37.0 24.5 37.0 80-81 33.213 37.0 37.0 37.0 14.0 37.0 82-83 33.199 37.0 37.0 37.0 14.0 37.0 84-85 33.2195 37.0 37.0 37.0 18.0 37.0 86-87 33.169250000000005 37.0 37.0 37.0 14.0 37.0 88-89 33.091125000000005 37.0 37.0 37.0 14.0 37.0 90-91 33.08 37.0 37.0 37.0 14.0 37.0 92-93 32.963 37.0 35.0 37.0 14.0 37.0 94-95 32.87675 37.0 35.0 37.0 14.0 37.0 96-97 32.790625 37.0 35.0 37.0 14.0 37.0 98-99 32.677875 37.0 33.0 37.0 14.0 37.0 100-101 32.56975 37.0 33.0 37.0 14.0 37.0 102-103 32.545249999999996 37.0 33.0 37.0 14.0 37.0 104-105 32.3565 37.0 33.0 37.0 14.0 37.0 106-107 31.938875000000003 37.0 33.0 37.0 14.0 37.0 108-109 32.0095 37.0 33.0 37.0 14.0 37.0 110-111 31.999375 37.0 33.0 37.0 14.0 37.0 112-113 31.828875 37.0 33.0 37.0 14.0 37.0 114-115 31.613875 37.0 33.0 37.0 14.0 37.0 116-117 31.336624999999998 37.0 33.0 37.0 8.0 37.0 118-119 31.1025 37.0 33.0 37.0 2.0 37.0 120-121 30.8275 37.0 33.0 37.0 2.0 37.0 122-123 30.416625 37.0 33.0 37.0 2.0 37.0 124-125 28.21625 37.0 17.5 37.0 2.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38.0 3 11.0 4 9.0 5 9.0 6 7.0 7 1.0 8 3.0 9 5.0 10 7.0 11 7.0 12 9.0 13 7.0 14 8.0 15 8.0 16 12.0 17 6.0 18 13.0 19 8.0 20 24.0 21 19.0 22 16.0 23 17.0 24 39.0 25 34.0 26 61.0 27 79.0 28 74.0 29 92.0 30 100.0 31 124.0 32 148.0 33 179.0 34 260.0 35 518.0 36 2048.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.950000000000003 12.075 29.9 30.075000000000003 2 24.425 12.35 27.075 36.15 3 21.224999999999998 14.025000000000002 33.074999999999996 31.674999999999997 4 22.825 12.25 30.875000000000004 34.050000000000004 5 24.425 14.000000000000002 26.400000000000002 35.175 6 27.224999999999998 17.75 30.099999999999998 24.925 7 18.675 25.4 41.775 14.149999999999999 8 14.85 27.925 40.825 16.400000000000002 9 17.175 26.900000000000002 39.375 16.55 10-11 17.9 25.7875 38.550000000000004 17.7625 12-13 18.625 26.674999999999997 37.0375 17.6625 14-15 17.6375 24.5125 37.6875 20.1625 16-17 18.7375 26.424999999999997 34.612500000000004 20.225 18-19 18.387500000000003 26.75 33.6125 21.25 20-21 18.099999999999998 25.362499999999997 33.887499999999996 22.650000000000002 22-23 18.9375 26.075 34.2125 20.775 24-25 19.037499999999998 27.187499999999996 33.15 20.625 26-27 19.2625 26.9625 31.825 21.95 28-29 19.7625 25.6125 30.8 23.825 30-31 18.6625 26.787499999999998 32.9375 21.6125 32-33 20.5375 27.275 31.525 20.6625 34-35 18.9 26.1125 33.225 21.762500000000003 36-37 18.7 24.9375 33.300000000000004 23.0625 38-39 19.1 26.450000000000003 32.5125 21.9375 40-41 19.7125 24.275 31.624999999999996 24.3875 42-43 23.3 25.0125 29.799999999999997 21.8875 44-45 21.912499999999998 24.825 30.175 23.0875 46-47 21.8625 25.874999999999996 29.275000000000002 22.9875 48-49 19.275000000000002 28.9125 29.9 21.912499999999998 50-51 20.75 25.4875 30.6375 23.125 52-53 21.762500000000003 25.674999999999997 30.562499999999996 22.0 54-55 20.0875 27.675 31.3125 20.925 56-57 20.025000000000002 26.275 32.05 21.65 58-59 20.95 25.575 30.9625 22.5125 60-61 18.75 28.012500000000003 30.95 22.287499999999998 62-63 21.712500000000002 25.724999999999998 28.599999999999998 23.962500000000002 64-65 21.087500000000002 25.2875 28.9125 24.712500000000002 66-67 20.3 27.2625 31.0125 21.425 68-69 21.0 27.1 28.999999999999996 22.900000000000002 70-71 20.2125 28.125 29.15 22.5125 72-73 20.5 27.3875 28.625 23.4875 74-75 21.6625 25.825 30.425 22.0875 76-77 22.025 27.6 26.724999999999998 23.65 78-79 21.3125 28.4 27.6375 22.650000000000002 80-81 23.549999999999997 27.025 28.3125 21.1125 82-83 21.95 25.1875 28.712500000000002 24.15 84-85 24.1375 26.0 26.400000000000002 23.4625 86-87 24.1375 26.7125 27.025 22.125 88-89 22.45 26.137500000000003 28.499999999999996 22.912499999999998 90-91 23.1 28.037499999999998 27.5125 21.349999999999998 92-93 23.05 25.8125 29.825000000000003 21.3125 94-95 23.674999999999997 27.0 26.674999999999997 22.650000000000002 96-97 24.1625 26.387500000000003 27.987499999999997 21.462500000000002 98-99 23.400000000000002 25.4875 26.525 24.587500000000002 100-101 24.4875 27.0625 26.3125 22.1375 102-103 24.099999999999998 27.212500000000002 26.275 22.412499999999998 104-105 23.474999999999998 27.1625 28.1 21.2625 106-107 23.8375 26.525 27.725 21.912499999999998 108-109 24.975 26.1625 26.687499999999996 22.175 110-111 25.0 28.1875 26.05 20.7625 112-113 24.212500000000002 26.137500000000003 26.974999999999998 22.675 114-115 25.2375 27.85 25.7625 21.15 116-117 25.912499999999998 26.775 26.224999999999998 21.087500000000002 118-119 26.7125 26.575 26.3 20.4125 120-121 25.924999999999997 26.5625 26.8375 20.674999999999997 122-123 25.5125 27.425 26.6 20.4625 124-125 24.962500000000002 26.6625 26.737499999999997 21.637500000000003 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4.0 1 2.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.5 19 2.5 20 3.5 21 3.5 22 6.5 23 12.0 24 19.0 25 26.0 26 28.5 27 36.0 28 46.5 29 55.0 30 64.0 31 70.5 32 82.5 33 95.0 34 118.5 35 122.0 36 115.0 37 126.5 38 144.5 39 147.0 40 149.5 41 191.5 42 212.5 43 191.5 44 176.0 45 169.0 46 154.5 47 133.5 48 127.5 49 132.0 50 110.0 51 108.0 52 108.0 53 82.5 54 64.0 55 59.0 56 61.5 57 59.0 58 49.0 59 37.0 60 35.5 61 35.0 62 32.5 63 29.0 64 24.0 65 19.0 66 14.0 67 12.5 68 14.5 69 12.5 70 9.0 71 8.0 72 6.0 73 4.0 74 7.0 75 9.5 76 7.0 77 4.5 78 3.0 79 1.5 80 1.0 81 0.5 82 1.5 83 1.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.55 #Duplication Level Percentage of deduplicated Percentage of total 1 97.6467477525119 92.325 2 1.4013749338974086 2.65 3 0.370174510840825 1.05 4 0.26441036488630354 1.0 5 0.07932310946589106 0.375 6 0.07932310946589106 0.44999999999999996 7 0.026441036488630353 0.17500000000000002 8 0.0 0.0 9 0.0 0.0 >10 0.13220518244315177 1.975 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCTTCATATTTAGTTTTATCTATTAATCGATAGTATCTACCGGCGCGAAC 22 0.5499999999999999 No Hit TCTTCGTATTTAGTTTTATCTATTAATCGATAGTATCTACCGGCGCGAAC 20 0.5 No Hit TGTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTTATCTATTAATCG 14 0.35000000000000003 No Hit TCTTCCTATTTAGTTTTATCTATTAATCGATAGTATCTACCGGCGCGAAC 13 0.325 No Hit TTGTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTTATCTATTAATC 10 0.25 No Hit GTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTTATCTATTAATCGA 7 0.17500000000000002 No Hit TTTGTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTTATCTATTAAT 6 0.15 No Hit CCACCGAATTGTAATACAGAATCATCCCCAAAGATTTCGGTCAGAGCTGG 6 0.15 No Hit TCTTTGTATTTAGTTTTATCTATTAATCGATAGTATCTACCGGCGCGAAC 6 0.15 No Hit TCTTTATATTTAGTTTTATCTATTAATCGATAGTATCTACCGGCGCGAAC 5 0.125 No Hit CCACCAAATTGTAATACAGAATCATCCCCAAAGATTTCGGTCAGAGCTGG 5 0.125 No Hit TCTTCTTATTTAGTTTTATCTATTAATCGATAGTATCTACCGGCGCGAAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.075 0.0 0.0 0.0 0.0 2 0.075 0.0 0.0 0.0 0.0 3 0.1 0.0 0.0 0.0 0.0 4 0.125 0.0 0.0 0.0 0.0 5 0.15 0.0 0.0 0.0 0.0 6 0.175 0.0 0.0 0.0 0.0 7 0.375 0.0 0.0 0.0 0.0 8 0.425 0.0 0.0 0.0 0.0 9 0.475 0.0 0.0 0.0 0.0 10-11 0.475 0.0 0.0 0.0 0.0 12-13 0.475 0.0 0.0 0.0 0.0 14-15 0.475 0.0 0.0 0.0 0.0 16-17 0.475 0.0 0.0 0.0 0.0 18-19 0.525 0.0 0.0 0.0 0.0 20-21 0.525 0.0 0.0 0.0 0.0 22-23 0.575 0.0 0.0 0.0 0.0 24-25 0.725 0.0 0.0 0.0 0.0 26-27 0.725 0.0 0.0 0.0 0.0 28-29 0.8125 0.0 0.0 0.0 0.0 30-31 0.85 0.0 0.0 0.0 0.0 32-33 0.8875 0.0 0.0 0.0 0.0 34-35 0.925 0.0 0.0 0.0 0.0 36-37 0.9875 0.0 0.0 0.0 0.0 38-39 1.2375 0.0 0.0 0.0 0.0 40-41 1.4375 0.0 0.0 0.0 0.0 42-43 1.5625 0.0 0.0 0.0 0.0 44-45 1.725 0.0 0.0 0.0 0.0 46-47 1.7875 0.0 0.0 0.0 0.0 48-49 1.8875000000000002 0.0 0.0 0.0 0.0 50-51 2.075 0.0 0.0 0.0 0.0 52-53 2.175 0.0 0.0 0.0 0.0 54-55 2.3875 0.0 0.0 0.0 0.0 56-57 2.45 0.0 0.0 0.0 0.0 58-59 2.5875 0.0 0.0 0.0 0.0 60-61 2.7874999999999996 0.0 0.0 0.0 0.0 62-63 2.9749999999999996 0.0 0.0 0.0 0.0 64-65 3.1625 0.0 0.0 0.0 0.0 66-67 3.4625 0.0 0.0 0.0 0.0 68-69 3.7625 0.0 0.0 0.0 0.0 70-71 4.225 0.0 0.0 0.0 0.0 72-73 4.65 0.0 0.0 0.0 0.0 74-75 5.05 0.0 0.0 0.0 0.0 76-77 5.5125 0.0 0.0 0.0 0.0 78-79 6.2 0.0 0.0 0.0 0.0 80-81 7.1125 0.0 0.0 0.0 0.0 82-83 7.9125 0.0 0.0 0.0 0.0 84-85 8.5 0.0 0.0 0.0 0.0 86-87 9.3625 0.0 0.0 0.0 0.0 88-89 10.2375 0.0 0.0 0.0 0.0 90-91 11.3625 0.0 0.0 0.0 0.0 92-93 12.175 0.0 0.0 0.0 0.0 94-95 13.0 0.0 0.0 0.0 0.0 96-97 13.75 0.0 0.0 0.0 0.0 98-99 14.4625 0.0 0.0 0.0 0.0 100-101 15.1875 0.0 0.0 0.0 0.0 102-103 16.1 0.0 0.0 0.0 0.0 104-105 16.875 0.0 0.0 0.0 0.0 106-107 17.8125 0.0 0.0 0.0 0.0 108-109 19.112499999999997 0.0 0.0 0.0 0.0 110-111 20.25 0.0 0.0 0.0 0.0 112-113 21.3875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1417749 spots for ERR3317431.sra Written 1417749 spots for ERR3317431.sra Read 1417749 spots for ERR3317431.sra Written 1417749 spots for ERR3317431.sra Read 1417749 spots for ERR3317431.sra Written 1417749 spots for ERR3317431.sra Read 1417749 spots for ERR3317431.sra Written 1417749 spots for ERR3317431.sra Read 1417749 spots for ERR3317431.sra Written 1417749 spots for ERR3317431.sra Read 1417749 spots for ERR3317431.sra Written 1417749 spots for ERR3317431.sra Read 1417749 spots for ERR3317431.sra Written 1417749 spots for ERR3317431.sra Read 1417749 spots for ERR3317431.sra Written 1417749 spots for ERR3317431.sra Read 1417749 spots for ERR3317431.sra Written 1417749 spots for ERR3317431.sra Read 1417749 spots for ERR3317431.sra Written 1417749 spots for ERR3317431.sra Read 1417749 spots for ERR3317431.sra Written 1417749 spots for ERR3317431.sra Read 1417749 spots for ERR3317431.sra Written 1417749 spots for ERR3317431.sra Read 1417749 spots for ERR3317431.sra Written 1417749 spots for ERR3317431.sra Read 1417749 spots for ERR3317431.sra Written 1417749 spots for ERR3317431.sra Read 1417749 spots for ERR3317431.sra Written 1417749 spots for ERR3317431.sra Read 1417749 spots for ERR3317431.sra Written 1417749 spots for ERR3317431.sra Read 1417749 spots for ERR3317431.sra Written 1417749 spots for ERR3317431.sra Read 1417761 spots for ERR3317431.sra Written 1417761 spots for ERR3317431.sra Read 1417749 spots for ERR3317431.sra Written 1417749 spots for ERR3317431.sra Read 1417749 spots for ERR3317431.sra Written 1417749 spots for ERR3317431.sra SRR ids: ['ERR3317431.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_by91m_qa ERR3317431.sra spots: 28354992 blocks: [[1, 1417749], [1417750, 2835498], [2835499, 4253247], [4253248, 5670996], [5670997, 7088745], [7088746, 8506494], [8506495, 9924243], [9924244, 11341992], [11341993, 12759741], [12759742, 14177490], [14177491, 15595239], [15595240, 17012988], [17012989, 18430737], [18430738, 19848486], [19848487, 21266235], [21266236, 22683984], [22683985, 24101733], [24101734, 25519482], [25519483, 26937231], [26937232, 28354992]] ERR3317431 file size 8146974 ERR3317431 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR3317431 ERR3317431_1.fastq ERR3317431_2.fastq Input file: ERR3317431_1.fastq Paired file: ERR3317431_2.fastq trimmed: ERR3317431-trimmed-pair1.fastq, ERR3317431-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Dec 10 09:11:35 2024 >> started Tue Dec 10 09:12:04 2024 >> done (28.971s) 28354992 read pairs processed; of these: 358102 ( 1.26%) short read pairs filtered out after trimming by size control 293336 ( 1.03%) empty read pairs filtered out after trimming by size control 27703554 (97.70%) read pairs available; of these: 15068710 (54.39%) trimmed read pairs available after processing 12634844 (45.61%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3763 0.01% 19 6376 0.02% 20 4721 0.02% 21 5957 0.02% 22 12184 0.04% 23 11905 0.04% 24 5302 0.02% 25 4422 0.02% 26 5956 0.02% 27 8953 0.03% 28 8197 0.03% 29 5539 0.02% 30 5301 0.02% 31 6442 0.02% 32 6371 0.02% 33 6784 0.02% 34 8146 0.03% 35 8563 0.03% 36 9499 0.03% 37 11100 0.04% 38 74068 0.27% 39 13470 0.05% 40 12649 0.05% 41 11936 0.04% 42 14518 0.05% 43 13971 0.05% 44 15936 0.06% 45 17593 0.06% 46 19021 0.07% 47 28248 0.10% 48 24345 0.09% 49 25417 0.09% 50 24371 0.09% 51 26501 0.10% 52 24945 0.09% 53 26842 0.10% 54 27942 0.10% 55 31264 0.11% 56 35214 0.13% 57 34273 0.12% 58 36210 0.13% 59 37538 0.14% 60 40878 0.15% 61 41272 0.15% 62 43037 0.16% 63 46312 0.17% 64 48382 0.17% 65 53265 0.19% 66 55026 0.20% 67 60338 0.22% 68 62104 0.22% 69 68956 0.25% 70 68261 0.25% 71 79238 0.29% 72 80614 0.29% 73 84622 0.31% 74 88798 0.32% 75 89667 0.32% 76 95920 0.35% 77 101149 0.37% 78 115324 0.42% 79 124097 0.45% 80 116299 0.42% 81 113197 0.41% 82 119189 0.43% 83 119248 0.43% 84 133034 0.48% 85 137161 0.50% 86 143201 0.52% 87 150085 0.54% 88 149289 0.54% 89 218204 0.79% 90 144247 0.52% 91 139364 0.50% 92 135635 0.49% 93 135474 0.49% 94 165082 0.60% 95 141854 0.51% 96 147646 0.53% 97 169725 0.61% 98 150185 0.54% 99 151920 0.55% 100 152624 0.55% 101 162359 0.59% 102 160454 0.58% 103 163509 0.59% 104 167736 0.61% 105 180245 0.65% 106 196886 0.71% 107 190940 0.69% 108 206131 0.74% 109 278050 1.00% 110 216459 0.78% 111 213176 0.77% 112 226310 0.82% 113 220276 0.80% 114 225684 0.81% 115 238914 0.86% 116 249656 0.90% 117 264655 0.96% 118 284521 1.03% 119 317744 1.15% 120 372873 1.35% 121 446771 1.61% 122 589530 2.13% 123 940366 3.39% 124 3353789 12.11% 125 12634844 45.61% 27703554 reads passed initial QC criterion=sequence-density sequence-density=0.86 sequence-density-rank=1 fanout-score=53.43 fanout-score-rank=6 prefix-density=0.92 prefix-fanout=50.0 sequence=AGATCGGAAGAGCACACC criterion=fanout-score sequence-density=0.15 sequence-density-rank=22 fanout-score=83.62 fanout-score-rank=1 prefix-density=0.73 prefix-fanout=16.8 sequence=AAAAAAATAAAAAA criterion=sequence-density sequence-density=1.04 sequence-density-rank=1 fanout-score=32.53 fanout-score-rank=10 prefix-density=1.06 prefix-fanout=31.8 sequence=GGTGTGCTCTTCCGATCT criterion=fanout-score sequence-density=0.02 sequence-density-rank=28 fanout-score=136.95 fanout-score-rank=1 prefix-density=0.28 prefix-fanout=10.0 sequence=TTTTCCTTTTTGTTTGTTTCGAGAAAGATATCGTCCGATTCTCCTTCTATTGATTCTTTTCCGATCGAGATGTATGGATCCATGTGTCTACATACCTAGATTCTGTTCATGGATTAACGAAAATGTGCAAGAGCTCTATTTGCCTCTGCCATTCTATGAGTCGCTTCCTTTTTGCGTATGGCACCCCCACTCCCTTTGGCAGCATCTACTAATTCGGAACTTAATTTGAAAGCCATATTTCGACCCGGACGCTTTTGGGATGCTTCTAATAACCAACGAATGGCAAGTGCTCTTCCTTGTTTAGATCCTATTTCAATCGGAACTTTCCGCGTCGATCCTTTTTTATTACGTCTTGTTTTTACTCCTATATTGGGAGTTACTCTACGTATTGCTTGACGTAAAACCAATAGTGGATTTGTTTCTGTCTTTTGTTGAATCTTTTTGACGGCTCGATAGAGAATTTGATAAGCCAATGATTTTTTTCCGTCTTTCATA ERR3317431 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 10 09:12:44 Started mapping on | Dec 10 09:12:44 Finished on | Dec 10 09:14:25 Mapping speed, Million of reads per hour | 987.45 Number of input reads | 27703554 Average input read length | 227 UNIQUE READS: Uniquely mapped reads number | 22297919 Uniquely mapped reads % | 80.49% Average mapped length | 227.78 Number of splices: Total | 7929552 Number of splices: Annotated (sjdb) | 7302000 Number of splices: GT/AG | 7787503 Number of splices: GC/AG | 119105 Number of splices: AT/AC | 4023 Number of splices: Non-canonical | 18921 Mismatch rate per base, % | 0.65% Deletion rate per base | 0.02% Deletion average length | 1.15 Insertion rate per base | 0.01% Insertion average length | 1.42 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 4387920 % of reads mapped to multiple loci | 15.84% Number of reads mapped to too many loci | 49274 % of reads mapped to too many loci | 0.18% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.73% % of reads unmapped: other | 0.77% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1079577 1079577 1079577 N_multimapping 4387920 4387920 4387920 N_noFeature 4300110 6354810 19621052 N_ambiguous 1432887 944918 51444 UnstrandedReadsAssigned:16564922 PositiveStrandReadsAssigned:14998191 NegativeStrandReadsAssigned:2625423 Dataset is classified positive stranded MeadianReadLen=125 20thPercentileLength=105 echo kmer=101 ERR3317431 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR3317431-trimmed-pair1.fastq ERR3317431-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 27,703,554 reads, 19,535,711 reads pseudoaligned [quant] estimated average fragment length: 171.23 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,150 rounds 52973 ERR3317431.ke.tsv 35125 ERR3317431.se.tsv 88098 total ==> ERR3317431.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 765.888 0 0 PNS24247 1044 873.77 4.19903 0.336897 PNS24249 1928 1757.77 18.5892 0.741385 PNS24246 1044 873.77 4.19903 0.336897 PNS24248 1044 873.77 4.19903 0.336897 PNS24244 1471 1300.77 411.814 22.1945 PNS24243 293 138.328 0 0 KQK14069 1603 1432.77 21623.8 1058.04 KQK14071 474 306.693 67.9792 15.5388 ==> ERR3317431.se.tsv <== BRADI_1g14170v3 22832 BRADI_1g53295v3 100 BRADI_1g59795v3 578 BRADI_1g07683v3 1 BRADI_1g00485v3 25 BRADI_1g20270v3 519 BRADI_1g74790v3 380 BRADI_1g09890v3 1 BRADI_1g77505v3 243 BRADI_1g48960v3 0 ERR3317431 completed mapping pipeline successfully