Starting /dee2/code/volunteer_pipeline.sh ERR3317432 current disk space = 1525347295232 free memory = 1526193344 ERR3317432 SRAfilesize 8d7a9b12172d7f808e8f917cf342290a ERR3317432.sra ERR3317432.sra file validated ERR3317432 is paired end ERR3317432 is conventional basespace ERR3317432 read1 length is 125 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR3317432_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.996 33.0 33.0 33.0 33.0 33.0 2 32.2565 33.0 33.0 33.0 33.0 33.0 3 31.77225 33.0 33.0 33.0 27.0 33.0 4 32.05725 33.0 33.0 33.0 33.0 33.0 5 32.13725 33.0 33.0 33.0 33.0 33.0 6 35.0225 37.0 37.0 37.0 27.0 37.0 7 35.38975 37.0 37.0 37.0 33.0 37.0 8 35.6475 37.0 37.0 37.0 33.0 37.0 9 35.71525 37.0 37.0 37.0 33.0 37.0 10-11 35.809375 37.0 37.0 37.0 33.0 37.0 12-13 35.861374999999995 37.0 37.0 37.0 33.0 37.0 14-15 35.838 37.0 37.0 37.0 33.0 37.0 16-17 35.79625 37.0 37.0 37.0 33.0 37.0 18-19 35.710750000000004 37.0 37.0 37.0 33.0 37.0 20-21 35.8475 37.0 37.0 37.0 33.0 37.0 22-23 35.860875 37.0 37.0 37.0 33.0 37.0 24-25 35.8575 37.0 37.0 37.0 33.0 37.0 26-27 35.815375 37.0 37.0 37.0 33.0 37.0 28-29 35.741 37.0 37.0 37.0 33.0 37.0 30-31 35.727625 37.0 37.0 37.0 33.0 37.0 32-33 35.665625 37.0 37.0 37.0 33.0 37.0 34-35 35.668625000000006 37.0 37.0 37.0 33.0 37.0 36-37 35.59275 37.0 37.0 37.0 33.0 37.0 38-39 35.453125 37.0 37.0 37.0 33.0 37.0 40-41 35.45925 37.0 37.0 37.0 33.0 37.0 42-43 35.463375 37.0 37.0 37.0 33.0 37.0 44-45 35.5465 37.0 37.0 37.0 33.0 37.0 46-47 35.46625 37.0 37.0 37.0 33.0 37.0 48-49 35.4825 37.0 37.0 37.0 33.0 37.0 50-51 35.39 37.0 37.0 37.0 33.0 37.0 52-53 35.339125 37.0 37.0 37.0 33.0 37.0 54-55 35.238625 37.0 37.0 37.0 33.0 37.0 56-57 35.304874999999996 37.0 37.0 37.0 33.0 37.0 58-59 35.185500000000005 37.0 37.0 37.0 33.0 37.0 60-61 35.178 37.0 37.0 37.0 33.0 37.0 62-63 35.078 37.0 37.0 37.0 33.0 37.0 64-65 35.1995 37.0 37.0 37.0 33.0 37.0 66-67 35.10575 37.0 37.0 37.0 33.0 37.0 68-69 35.041875000000005 37.0 37.0 37.0 33.0 37.0 70-71 35.014375 37.0 37.0 37.0 33.0 37.0 72-73 34.994125 37.0 37.0 37.0 33.0 37.0 74-75 34.939499999999995 37.0 37.0 37.0 33.0 37.0 76-77 34.881875 37.0 37.0 37.0 30.0 37.0 78-79 34.736625000000004 37.0 37.0 37.0 27.0 37.0 80-81 34.740875 37.0 37.0 37.0 30.0 37.0 82-83 34.550875000000005 37.0 37.0 37.0 27.0 37.0 84-85 34.489625000000004 37.0 37.0 37.0 27.0 37.0 86-87 34.531875 37.0 37.0 37.0 27.0 37.0 88-89 34.336625 37.0 37.0 37.0 27.0 37.0 90-91 34.218375 37.0 37.0 37.0 27.0 37.0 92-93 34.2705 37.0 37.0 37.0 27.0 37.0 94-95 34.284125 37.0 37.0 37.0 27.0 37.0 96-97 34.300875000000005 37.0 37.0 37.0 27.0 37.0 98-99 34.202375 37.0 37.0 37.0 27.0 37.0 100-101 34.232875 37.0 37.0 37.0 27.0 37.0 102-103 33.97775 37.0 37.0 37.0 27.0 37.0 104-105 34.076499999999996 37.0 37.0 37.0 27.0 37.0 106-107 33.973124999999996 37.0 37.0 37.0 27.0 37.0 108-109 33.9805 37.0 37.0 37.0 27.0 37.0 110-111 33.977125 37.0 37.0 37.0 27.0 37.0 112-113 33.6685 37.0 37.0 37.0 27.0 37.0 114-115 33.65675 37.0 37.0 37.0 24.5 37.0 116-117 33.557874999999996 37.0 37.0 37.0 24.5 37.0 118-119 33.411874999999995 37.0 37.0 37.0 24.5 37.0 120-121 32.776375 37.0 37.0 37.0 14.0 37.0 122-123 32.69425 37.0 37.0 37.0 14.0 37.0 124-125 31.138749999999998 37.0 35.0 37.0 2.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 0.0 5 2.0 6 1.0 7 4.0 8 0.0 9 4.0 10 0.0 11 5.0 12 4.0 13 2.0 14 5.0 15 4.0 16 4.0 17 3.0 18 4.0 19 9.0 20 7.0 21 13.0 22 12.0 23 21.0 24 21.0 25 22.0 26 28.0 27 36.0 28 56.0 29 74.0 30 96.0 31 117.0 32 155.0 33 195.0 34 263.0 35 522.0 36 2310.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 23.025000000000002 26.275 22.575 28.125 2 28.475 24.525 20.075000000000003 26.924999999999997 3 29.1123370110331 25.175526579739216 18.50551654964895 27.206619859578733 4 27.781945486371594 23.455863965991497 19.529882470617654 29.232308077019255 5 29.2 26.650000000000002 16.75 27.400000000000002 6 29.775000000000002 28.15 17.2 24.875 7 28.075 26.450000000000003 20.825 24.65 8 32.75 25.3 23.95 18.0 9 32.675 28.050000000000004 23.35 15.925 10-11 32.375 26.387500000000003 23.400000000000002 17.837500000000002 12-13 30.412499999999998 24.65 25.174999999999997 19.7625 14-15 27.5625 27.462500000000002 24.9 20.075000000000003 16-17 26.700000000000003 27.250000000000004 25.374999999999996 20.674999999999997 18-19 24.462500000000002 27.6625 26.950000000000003 20.925 20-21 23.6125 28.875 26.825 20.6875 22-23 24.962500000000002 27.712500000000002 26.025 21.3 24-25 24.6125 27.537499999999998 26.575 21.275 26-27 24.525 27.237499999999997 26.3625 21.875 28-29 25.587500000000002 27.675 25.337500000000002 21.4 30-31 23.5125 28.775000000000002 27.525 20.1875 32-33 23.35 28.225 26.787499999999998 21.637500000000003 34-35 24.575 27.450000000000003 26.625 21.349999999999998 36-37 25.0625 27.675 26.025 21.2375 38-39 23.4375 28.512500000000003 27.5875 20.4625 40-41 25.337500000000002 27.187499999999996 26.924999999999997 20.549999999999997 42-43 25.3125 27.1625 26.987499999999997 20.5375 44-45 25.374999999999996 28.0875 26.3625 20.175 46-47 25.224999999999998 27.0 25.525 22.25 48-49 25.7625 27.500000000000004 26.0 20.7375 50-51 23.2375 27.375 27.200000000000003 22.1875 52-53 24.212500000000002 28.012500000000003 27.212500000000002 20.5625 54-55 24.099999999999998 27.2625 27.35 21.2875 56-57 23.4625 28.212500000000002 26.5875 21.7375 58-59 24.3 28.962500000000002 25.837500000000002 20.9 60-61 24.3125 27.125 27.275 21.2875 62-63 23.575 27.950000000000003 26.6 21.875 64-65 24.1875 27.6 26.6 21.6125 66-67 23.525 28.249999999999996 26.35 21.875 68-69 24.1125 27.3625 26.987499999999997 21.5375 70-71 23.95 30.475 24.462500000000002 21.1125 72-73 23.8375 29.4875 26.187500000000004 20.4875 74-75 24.325 29.5375 26.1125 20.025000000000002 76-77 23.025000000000002 29.725 25.387500000000003 21.8625 78-79 22.525000000000002 30.4625 26.3625 20.65 80-81 24.1625 29.675 26.25 19.9125 82-83 23.724999999999998 28.8625 26.4125 21.0 84-85 23.8625 28.349999999999998 27.0875 20.7 86-87 23.2875 30.587500000000002 26.0375 20.0875 88-89 23.9125 29.7375 25.7125 20.6375 90-91 24.025 29.6625 25.074999999999996 21.2375 92-93 24.2375 29.2 25.374999999999996 21.1875 94-95 24.1625 29.675 25.45 20.7125 96-97 24.4375 28.875 24.675 22.0125 98-99 23.200000000000003 29.7 25.974999999999998 21.125 100-101 24.8 29.825000000000003 24.075 21.3 102-103 23.25 30.225 25.387500000000003 21.1375 104-105 24.4375 29.812499999999996 25.0 20.75 106-107 23.8625 29.5875 25.0 21.55 108-109 24.3625 29.975 23.8375 21.825 110-111 23.425 30.2 24.7875 21.587500000000002 112-113 23.125 29.875 24.4375 22.5625 114-115 24.2875 28.15 25.0375 22.525000000000002 116-117 24.0 30.4375 23.7375 21.825 118-119 24.075 30.2125 24.8125 20.9 120-121 23.825 29.7125 24.25 22.2125 122-123 23.575 29.462500000000002 24.25 22.7125 124-125 24.975 29.349999999999998 23.875 21.8 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 2.5 2 1.5 3 0.0 4 1.0 5 1.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.0 18 0.0 19 1.0 20 2.0 21 1.5 22 1.5 23 3.0 24 4.5 25 7.5 26 11.0 27 13.0 28 19.0 29 24.0 30 37.0 31 45.5 32 45.5 33 59.0 34 69.5 35 73.0 36 86.5 37 99.0 38 97.5 39 124.5 40 151.0 41 169.0 42 200.0 43 222.5 44 197.5 45 173.0 46 177.0 47 171.0 48 180.5 49 171.0 50 150.5 51 136.0 52 112.5 53 110.0 54 105.0 55 81.5 56 70.5 57 57.0 58 51.0 59 48.5 60 42.0 61 41.0 62 39.0 63 37.5 64 35.5 65 30.5 66 28.0 67 24.5 68 24.5 69 21.5 70 13.0 71 14.0 72 14.0 73 13.5 74 10.0 75 7.0 76 7.0 77 4.0 78 2.5 79 4.0 80 5.5 81 4.0 82 2.5 83 2.5 84 1.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.3 4 0.025 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.35 #Duplication Level Percentage of deduplicated Percentage of total 1 97.0492690850027 89.625 2 1.570113697888468 2.9000000000000004 3 0.6226312939902545 1.725 4 0.32485110990795885 1.2 5 0.16242555495397942 0.75 6 0.0 0.0 7 0.02707092582566324 0.17500000000000002 8 0.02707092582566324 0.2 9 0.08121277747698971 0.675 >10 0.1353546291283162 2.75 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGCAAATGGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGAT 39 0.975 No Hit AGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGG 26 0.65 No Hit CCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTCGAGTT 18 0.44999999999999996 No Hit AGGCGGAAGAGTCCTCTTAATATTTATCTAATCTTATATAGGTTTCAGTA 16 0.4 No Hit GAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTCGAGTTCGC 11 0.27499999999999997 No Hit GCAAATGGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATC 9 0.22499999999999998 No Hit TGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTG 9 0.22499999999999998 No Hit TGTGAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTAT 9 0.22499999999999998 No Hit GTGTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAA 8 0.2 No Hit CCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTA 7 0.17500000000000002 No Hit CTCAGTAGAAGGTTCCCTCGGAACAATTATTATATATTTCAAGTTATTTC 5 0.125 No Hit CTTGCAAATGGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCG 5 0.125 No Hit TAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCG 5 0.125 No Hit AGCCAACTCTCAGTAGAAGGTTCCCTCGGAACAATTATTATATATTTCAA 5 0.125 No Hit TAAACCCATTGATAAAAAGAAAAAAAGAGGATAAAAGTTAGGGAATAAAA 5 0.125 No Hit CGGTTGTGACTCCATAGATAAAAATGAAAAAATTTATATCTTCGAGACCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.05 0.0 0.0 0.0 0.0 2 0.075 0.0 0.0 0.0 0.0 3 0.075 0.0 0.0 0.0 0.0 4 0.1 0.0 0.0 0.0 0.0 5 0.15 0.0 0.0 0.0 0.0 6 0.35 0.0 0.0 0.0 0.0 7 0.65 0.0 0.0 0.0 0.0 8 0.775 0.0 0.0 0.0 0.0 9 0.825 0.0 0.0 0.0 0.0 10-11 0.875 0.0 0.0 0.0 0.0 12-13 0.875 0.0 0.0 0.0 0.0 14-15 0.9 0.0 0.0 0.0 0.0 16-17 0.925 0.0 0.0 0.0 0.0 18-19 0.95 0.0 0.0 0.0 0.0 20-21 1.0125 0.0 0.0 0.0 0.0 22-23 1.075 0.0 0.0 0.0 0.0 24-25 1.15 0.0 0.0 0.0 0.0 26-27 1.175 0.0 0.0 0.0 0.0 28-29 1.175 0.0 0.0 0.0 0.0 30-31 1.2 0.0 0.0 0.0 0.0 32-33 1.225 0.0 0.0 0.0 0.0 34-35 1.225 0.0 0.0 0.0 0.0 36-37 1.325 0.0 0.0 0.0 0.0 38-39 1.4875 0.0 0.0 0.0 0.0 40-41 1.65 0.0 0.0 0.0 0.0 42-43 1.8125 0.0 0.0 0.0 0.0 44-45 1.9 0.0 0.0 0.0 0.0 46-47 2.0125 0.0 0.0 0.0 0.0 48-49 2.1500000000000004 0.0 0.0 0.0 0.0 50-51 2.2875 0.0 0.0 0.0 0.0 52-53 2.5 0.0 0.0 0.0 0.0 54-55 2.6375 0.0 0.0 0.0 0.0 56-57 2.9 0.0 0.0 0.0 0.0 58-59 3.2375 0.0 0.0 0.0 0.0 60-61 3.4749999999999996 0.0 0.0 0.0 0.0 62-63 3.75 0.0 0.0 0.0 0.0 64-65 4.0125 0.0 0.0 0.0 0.0 66-67 4.3375 0.0 0.0 0.0 0.0 68-69 4.7125 0.0 0.0 0.0 0.0 70-71 5.025 0.0 0.0 0.0 0.0 72-73 5.4 0.0 0.0 0.0 0.0 74-75 5.737500000000001 0.0 0.0 0.0 0.0 76-77 6.275 0.0 0.0 0.0 0.0 78-79 6.737500000000001 0.0 0.0 0.0 0.0 80-81 7.4375 0.0 0.0 0.0 0.0 82-83 8.15 0.0 0.0 0.0 0.0 84-85 8.8375 0.0 0.0 0.0 0.0 86-87 9.4625 0.0 0.0 0.0 0.0 88-89 10.5 0.0 0.0 0.0 0.0 90-91 11.5625 0.0 0.0 0.0 0.0 92-93 12.2125 0.0 0.0 0.0 0.0 94-95 12.9875 0.0 0.0 0.0 0.0 96-97 13.9 0.0 0.0 0.0 0.0 98-99 14.8875 0.0 0.0 0.0 0.0 100-101 15.575 0.0 0.0 0.0 0.0 102-103 16.6 0.0 0.0 0.0 0.0 104-105 17.5375 0.0 0.0 0.0 0.0 106-107 18.512500000000003 0.0 0.0 0.0 0.0 108-109 19.6 0.0 0.0 0.0 0.0 110-111 21.0375 0.0 0.0 0.0 0.0 112-113 22.1875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCAAAT 30 0.003934916 59.5 1 TGGAGTC 30 0.003934916 59.5 7 ATGGAGT 30 0.003934916 59.5 6 GAGTCCT 30 0.003934916 59.5 9 GCAAATG 30 0.003934916 59.5 2 AATGGAG 30 0.003934916 59.5 5 CAAATGG 30 0.003934916 59.5 3 GGAGTCC 30 0.003934916 59.5 8 AAATGGA 35 0.007243562 51.0 4 >>END_MODULE ERR3317432 read2 length is 125 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR3317432_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.751 33.0 33.0 33.0 33.0 33.0 2 31.784 33.0 33.0 33.0 33.0 33.0 3 31.8675 33.0 33.0 33.0 33.0 33.0 4 31.84125 33.0 33.0 33.0 33.0 33.0 5 31.89525 33.0 33.0 33.0 33.0 33.0 6 35.2765 37.0 37.0 37.0 33.0 37.0 7 35.1425 37.0 37.0 37.0 33.0 37.0 8 35.2445 37.0 37.0 37.0 33.0 37.0 9 35.379 37.0 37.0 37.0 33.0 37.0 10-11 35.24225 37.0 37.0 37.0 33.0 37.0 12-13 35.154375 37.0 37.0 37.0 33.0 37.0 14-15 35.13275 37.0 37.0 37.0 33.0 37.0 16-17 35.102625 37.0 37.0 37.0 33.0 37.0 18-19 34.930625000000006 37.0 37.0 37.0 33.0 37.0 20-21 34.909875 37.0 37.0 37.0 33.0 37.0 22-23 34.86875 37.0 37.0 37.0 33.0 37.0 24-25 34.894625000000005 37.0 37.0 37.0 33.0 37.0 26-27 34.762125 37.0 37.0 37.0 27.0 37.0 28-29 34.772375 37.0 37.0 37.0 30.0 37.0 30-31 34.79625 37.0 37.0 37.0 33.0 37.0 32-33 34.655 37.0 37.0 37.0 27.0 37.0 34-35 34.56625 37.0 37.0 37.0 27.0 37.0 36-37 34.6555 37.0 37.0 37.0 27.0 37.0 38-39 34.667500000000004 37.0 37.0 37.0 27.0 37.0 40-41 34.689875 37.0 37.0 37.0 27.0 37.0 42-43 34.653499999999994 37.0 37.0 37.0 27.0 37.0 44-45 34.540125 37.0 37.0 37.0 27.0 37.0 46-47 34.583 37.0 37.0 37.0 27.0 37.0 48-49 34.522125 37.0 37.0 37.0 27.0 37.0 50-51 34.377375 37.0 37.0 37.0 27.0 37.0 52-53 34.369375000000005 37.0 37.0 37.0 27.0 37.0 54-55 34.404250000000005 37.0 37.0 37.0 27.0 37.0 56-57 34.425625 37.0 37.0 37.0 27.0 37.0 58-59 34.432375 37.0 37.0 37.0 27.0 37.0 60-61 34.268125 37.0 37.0 37.0 27.0 37.0 62-63 34.207875 37.0 37.0 37.0 27.0 37.0 64-65 34.1635 37.0 37.0 37.0 27.0 37.0 66-67 34.171625000000006 37.0 37.0 37.0 27.0 37.0 68-69 34.160624999999996 37.0 37.0 37.0 27.0 37.0 70-71 33.9905 37.0 37.0 37.0 27.0 37.0 72-73 33.957375 37.0 37.0 37.0 27.0 37.0 74-75 33.804625 37.0 37.0 37.0 27.0 37.0 76-77 33.722625 37.0 37.0 37.0 27.0 37.0 78-79 33.71475 37.0 37.0 37.0 27.0 37.0 80-81 33.4015 37.0 37.0 37.0 18.0 37.0 82-83 33.485 37.0 37.0 37.0 24.5 37.0 84-85 33.343999999999994 37.0 37.0 37.0 18.0 37.0 86-87 33.41525 37.0 37.0 37.0 22.0 37.0 88-89 33.421625 37.0 37.0 37.0 24.5 37.0 90-91 33.395624999999995 37.0 37.0 37.0 22.0 37.0 92-93 33.226375000000004 37.0 37.0 37.0 14.0 37.0 94-95 33.274375 37.0 37.0 37.0 22.0 37.0 96-97 33.101124999999996 37.0 37.0 37.0 14.0 37.0 98-99 33.068124999999995 37.0 37.0 37.0 14.0 37.0 100-101 32.96575 37.0 37.0 37.0 14.0 37.0 102-103 32.936625 37.0 37.0 37.0 14.0 37.0 104-105 32.9285 37.0 37.0 37.0 14.0 37.0 106-107 32.67775 37.0 33.0 37.0 14.0 37.0 108-109 32.732 37.0 37.0 37.0 14.0 37.0 110-111 32.57625 37.0 33.0 37.0 14.0 37.0 112-113 32.621125 37.0 35.0 37.0 14.0 37.0 114-115 32.364625000000004 37.0 33.0 37.0 14.0 37.0 116-117 32.247125 37.0 33.0 37.0 14.0 37.0 118-119 32.081125 37.0 33.0 37.0 14.0 37.0 120-121 31.59225 37.0 33.0 37.0 8.0 37.0 122-123 31.310875 37.0 33.0 37.0 2.0 37.0 124-125 29.11775 37.0 27.5 37.0 2.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30.0 3 10.0 4 11.0 5 9.0 6 11.0 7 3.0 8 7.0 9 8.0 10 2.0 11 3.0 12 8.0 13 4.0 14 7.0 15 11.0 16 6.0 17 6.0 18 6.0 19 11.0 20 12.0 21 22.0 22 31.0 23 18.0 24 29.0 25 39.0 26 48.0 27 49.0 28 67.0 29 72.0 30 103.0 31 116.0 32 139.0 33 185.0 34 259.0 35 426.0 36 2232.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.3 13.100000000000001 33.15 26.450000000000003 2 24.375 12.5 26.674999999999997 36.449999999999996 3 20.3 12.5 32.475 34.725 4 21.224999999999998 12.725 30.4 35.65 5 23.25 15.299999999999999 25.55 35.9 6 24.65 19.25 30.75 25.35 7 16.7 25.900000000000002 43.35 14.05 8 15.0 27.950000000000003 39.574999999999996 17.474999999999998 9 16.75 27.6 38.824999999999996 16.825000000000003 10-11 17.349999999999998 26.8125 38.237500000000004 17.599999999999998 12-13 18.5625 28.499999999999996 34.725 18.212500000000002 14-15 17.075000000000003 26.575 36.7125 19.6375 16-17 18.5 27.750000000000004 33.4875 20.2625 18-19 18.2 28.025 32.2125 21.5625 20-21 18.85 25.912499999999998 31.8 23.4375 22-23 18.725 28.3875 30.85 22.037499999999998 24-25 18.35 29.562500000000004 31.0125 21.075 26-27 19.625 29.2 28.9125 22.2625 28-29 20.200000000000003 28.325 27.450000000000003 24.025 30-31 18.825 29.362500000000004 30.5 21.3125 32-33 20.775 29.262500000000003 28.7 21.2625 34-35 18.912499999999998 28.425 30.8 21.8625 36-37 19.1 26.825 30.1875 23.8875 38-39 19.650000000000002 28.249999999999996 29.549999999999997 22.55 40-41 20.2375 26.6125 27.987499999999997 25.162499999999998 42-43 23.0125 27.3625 26.924999999999997 22.7 44-45 22.400000000000002 27.487499999999997 27.287499999999998 22.825 46-47 21.075 28.6375 27.775 22.5125 48-49 20.575 30.4375 27.8375 21.15 50-51 20.3125 27.250000000000004 28.275 24.1625 52-53 21.45 27.712500000000002 27.1 23.7375 54-55 19.125 29.912499999999998 28.5625 22.400000000000002 56-57 20.075000000000003 27.474999999999998 29.8875 22.5625 58-59 21.825 27.1625 28.125 22.8875 60-61 19.6375 29.175 28.199999999999996 22.9875 62-63 21.275 27.8125 25.8625 25.05 64-65 21.3 26.3625 25.887500000000003 26.450000000000003 66-67 20.8125 27.762500000000003 29.012500000000003 22.412499999999998 68-69 21.1625 28.025 25.087500000000002 25.724999999999998 70-71 20.4125 29.4125 27.0125 23.1625 72-73 19.9875 28.287499999999998 27.3375 24.3875 74-75 20.9875 27.9125 27.9375 23.1625 76-77 22.237499999999997 29.6375 24.525 23.599999999999998 78-79 21.625 29.212500000000002 25.0625 24.099999999999998 80-81 22.8375 28.5625 26.5875 22.0125 82-83 21.712500000000002 26.85 26.6 24.837500000000002 84-85 23.575 27.625 25.25 23.549999999999997 86-87 25.05 28.0875 24.275 22.5875 88-89 22.25 26.887499999999996 26.4125 24.45 90-91 23.2625 28.625 25.4 22.7125 92-93 22.45 27.625 26.7625 23.1625 94-95 23.3375 27.925 24.887500000000003 23.849999999999998 96-97 24.25 28.3875 25.3 22.0625 98-99 23.35 27.750000000000004 24.975 23.925 100-101 23.5375 27.250000000000004 25.624999999999996 23.5875 102-103 24.5125 27.1625 24.474999999999998 23.849999999999998 104-105 22.85 27.250000000000004 26.9125 22.9875 106-107 25.4375 26.8 24.9875 22.775000000000002 108-109 24.775 27.987499999999997 25.05 22.1875 110-111 25.9625 29.15 23.1625 21.725 112-113 24.3125 27.525 25.15 23.0125 114-115 25.4375 27.8875 24.0 22.675 116-117 24.975 26.724999999999998 25.0375 23.2625 118-119 26.8 27.187499999999996 23.4875 22.525000000000002 120-121 24.375 28.625 25.7625 21.2375 122-123 25.85 29.262500000000003 24.3 20.5875 124-125 25.662499999999998 26.5 26.2125 21.625 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2.0 1 2.5 2 1.5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.0 10 1.0 11 0.5 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 1.0 21 2.0 22 4.5 23 8.0 24 13.5 25 18.0 26 19.0 27 23.5 28 31.5 29 35.5 30 41.5 31 60.0 32 73.0 33 75.5 34 104.5 35 122.0 36 116.0 37 128.0 38 144.0 39 166.0 40 175.0 41 200.0 42 235.0 43 221.0 44 194.0 45 170.5 46 150.0 47 149.0 48 132.0 49 110.0 50 112.5 51 118.0 52 115.0 53 90.5 54 73.5 55 66.5 56 54.5 57 52.5 58 47.0 59 36.0 60 36.0 61 33.5 62 31.0 63 29.0 64 24.0 65 19.0 66 14.5 67 17.5 68 17.5 69 16.0 70 11.5 71 8.0 72 8.0 73 5.0 74 5.5 75 6.0 76 4.5 77 4.0 78 3.0 79 2.0 80 1.5 81 2.0 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.05 #Duplication Level Percentage of deduplicated Percentage of total 1 97.63423710792132 91.825 2 1.3024986709197237 2.45 3 0.5050505050505051 1.425 4 0.13290802764486975 0.5 5 0.13290802764486975 0.625 6 0.053163211057947905 0.3 7 0.053163211057947905 0.35000000000000003 8 0.0 0.0 9 0.026581605528973953 0.22499999999999998 >10 0.1594896331738437 2.3 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCTTCATATTTAGTTTTATCTATTAATCGATAGTATCTACCGGCGCGAAC 31 0.775 No Hit TCTTCGTATTTAGTTTTATCTATTAATCGATAGTATCTACCGGCGCGAAC 15 0.375 No Hit TTGTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTTATCTATTAATC 13 0.325 No Hit TGTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTTATCTATTAATCG 12 0.3 No Hit TCTTCTTATTTAGTTTTATCTATTAATCGATAGTATCTACCGGCGCGAAC 11 0.27499999999999997 No Hit GTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTTATCTATTAATCGA 10 0.25 No Hit TCTTCCTATTTAGTTTTATCTATTAATCGATAGTATCTACCGGCGCGAAC 9 0.22499999999999998 No Hit TTCTCTTTTTATTTTGTTTCTTTTTATTTAGACCTTCTTCATATTTAGTT 7 0.17500000000000002 No Hit TTTTATTTTGTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTTATCT 7 0.17500000000000002 No Hit TCTTTATATTTAGTTTTATCTATTAATCGATAGTATCTACCGGCGCGAAC 6 0.15 No Hit CTTTTTATTTTGTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTTAT 6 0.15 No Hit AGACCTTCTTCATATTTAGTTTTATCTATTAATCGATAGTATCTACCGGC 5 0.125 No Hit CTTCATATTTAGTTTTATCTATTAATCGATAGTATCTACCGGCGCGAACT 5 0.125 No Hit TCATATTTAGTTTTATCTATTAATCGATAGTATCTACCGGCGCGAACTCG 5 0.125 No Hit TATTTTTTCTCTTTTTATTTTGTTTCTTTTTATTTAGACCTTCTTCATAT 5 0.125 No Hit TTTGCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.05 0.0 0.0 0.0 0.0 3 0.05 0.0 0.0 0.0 0.0 4 0.075 0.0 0.0 0.0 0.0 5 0.125 0.0 0.0 0.0 0.0 6 0.3 0.0 0.0 0.0 0.0 7 0.6 0.0 0.0 0.0 0.0 8 0.725 0.0 0.0 0.0 0.0 9 0.775 0.0 0.0 0.0 0.0 10-11 0.825 0.0 0.0 0.0 0.0 12-13 0.825 0.0 0.0 0.0 0.0 14-15 0.85 0.0 0.0 0.0 0.0 16-17 0.8625 0.0 0.0 0.0 0.0 18-19 0.875 0.0 0.0 0.0 0.0 20-21 0.9375 0.0 0.0 0.0 0.0 22-23 0.9874999999999999 0.0 0.0 0.0 0.0 24-25 1.0499999999999998 0.0 0.0 0.0 0.0 26-27 1.075 0.0 0.0 0.0 0.0 28-29 1.0875 0.0 0.0 0.0 0.0 30-31 1.125 0.0 0.0 0.0 0.0 32-33 1.15 0.0 0.0 0.0 0.0 34-35 1.15 0.0 0.0 0.0 0.0 36-37 1.2 0.0 0.0 0.0 0.0 38-39 1.3625 0.0 0.0 0.0 0.0 40-41 1.5 0.0 0.0 0.0 0.0 42-43 1.65 0.0 0.0 0.0 0.0 44-45 1.725 0.0 0.0 0.0 0.0 46-47 1.8375 0.0 0.0 0.0 0.0 48-49 1.9625 0.0 0.0 0.0 0.0 50-51 2.0999999999999996 0.0 0.0 0.0 0.0 52-53 2.2625 0.0 0.0 0.0 0.0 54-55 2.375 0.0 0.0 0.0 0.0 56-57 2.625 0.0 0.0 0.0 0.0 58-59 2.9625 0.0 0.0 0.0 0.0 60-61 3.2 0.0 0.0 0.0 0.0 62-63 3.5 0.0 0.0 0.0 0.0 64-65 3.7625 0.0 0.0 0.0 0.0 66-67 4.0625 0.0 0.0 0.0 0.0 68-69 4.425 0.0 0.0 0.0 0.0 70-71 4.762499999999999 0.0 0.0 0.0 0.0 72-73 5.1375 0.0 0.0 0.0 0.0 74-75 5.475 0.0 0.0 0.0 0.0 76-77 6.0375 0.0 0.0 0.0 0.0 78-79 6.5875 0.0 0.0 0.0 0.0 80-81 7.3 0.0 0.0 0.0 0.0 82-83 7.9875 0.0 0.0 0.0 0.0 84-85 8.675 0.0 0.0 0.0 0.0 86-87 9.274999999999999 0.0 0.0 0.0 0.0 88-89 10.225 0.0 0.0 0.0 0.0 90-91 11.2875 0.0 0.0 0.0 0.0 92-93 11.9375 0.0 0.0 0.0 0.0 94-95 12.7 0.0 0.0 0.0 0.0 96-97 13.6 0.0 0.0 0.0 0.0 98-99 14.600000000000001 0.0 0.0 0.0 0.0 100-101 15.2875 0.0 0.0 0.0 0.0 102-103 16.2875 0.0 0.0 0.0 0.0 104-105 17.237499999999997 0.0 0.0 0.0 0.0 106-107 18.2 0.0 0.0 0.0 0.0 108-109 19.3375 0.0 0.0 0.0 0.0 110-111 20.75 0.0 0.0 0.0 0.0 112-113 21.875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTTAGT 60 0.001158463 39.666668 8 TTTAGTT 65 0.0017172955 36.615383 9 CGATAGT 65 5.634549E-5 27.461538 28-29 GGCGCGA 65 5.634549E-5 27.461538 42-43 ATCTATT 65 5.634549E-5 27.461538 18-19 TTATCTA 65 5.634549E-5 27.461538 16-17 TTTTATC 65 5.634549E-5 27.461538 14-15 TCTACCG 65 5.634549E-5 27.461538 36-37 AGTATCT 65 5.634549E-5 27.461538 32-33 ATAGTAT 65 5.634549E-5 27.461538 30-31 TACCGGC 65 5.634549E-5 27.461538 38-39 TATCTAC 65 5.634549E-5 27.461538 34-35 TAATCGA 65 5.634549E-5 27.461538 24-25 AGTTTTA 65 5.634549E-5 27.461538 12-13 CCGGCGC 65 5.634549E-5 27.461538 40-41 CTATTAA 65 5.634549E-5 27.461538 20-21 CTCGAAT 55 7.095549E-4 27.045454 50-51 TGATCGC 55 7.095549E-4 27.045454 58-59 CGAATTT 55 7.095549E-4 27.045454 52-53 CCTTCCA 55 7.095549E-4 27.045454 64-65 >>END_MODULE Read 531345 spots for ERR3317432.sra Written 531345 spots for ERR3317432.sra Read 531345 spots for ERR3317432.sra Written 531345 spots for ERR3317432.sra Read 531345 spots for ERR3317432.sra Written 531345 spots for ERR3317432.sra Read 531345 spots for ERR3317432.sra Written 531345 spots for ERR3317432.sra Read 531345 spots for ERR3317432.sra Written 531345 spots for ERR3317432.sra Read 531345 spots for ERR3317432.sra Written 531345 spots for ERR3317432.sra Read 531345 spots for ERR3317432.sra Written 531345 spots for ERR3317432.sra Read 531345 spots for ERR3317432.sra Written 531345 spots for ERR3317432.sra Read 531345 spots for ERR3317432.sra Written 531345 spots for ERR3317432.sra Read 531345 spots for ERR3317432.sra Written 531345 spots for ERR3317432.sra Read 531345 spots for ERR3317432.sra Written 531345 spots for ERR3317432.sra Read 531345 spots for ERR3317432.sra Written 531345 spots for ERR3317432.sra Read 531345 spots for ERR3317432.sra Written 531345 spots for ERR3317432.sra Read 531345 spots for ERR3317432.sra Written 531345 spots for ERR3317432.sra Read 531345 spots for ERR3317432.sra Written 531345 spots for ERR3317432.sra Read 531345 spots for ERR3317432.sra Written 531345 spots for ERR3317432.sra Read 531345 spots for ERR3317432.sra Written 531345 spots for ERR3317432.sra Read 531351 spots for ERR3317432.sra Written 531351 spots for ERR3317432.sra Read 531345 spots for ERR3317432.sra Written 531345 spots for ERR3317432.sra Read 531345 spots for ERR3317432.sra Written 531345 spots for ERR3317432.sra SRR ids: ['ERR3317432.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_6vihz6ew ERR3317432.sra spots: 10626906 blocks: [[1, 531345], [531346, 1062690], [1062691, 1594035], [1594036, 2125380], [2125381, 2656725], [2656726, 3188070], [3188071, 3719415], [3719416, 4250760], [4250761, 4782105], [4782106, 5313450], [5313451, 5844795], [5844796, 6376140], [6376141, 6907485], [6907486, 7438830], [7438831, 7970175], [7970176, 8501520], [8501521, 9032865], [9032866, 9564210], [9564211, 10095555], [10095556, 10626906]] ERR3317432 file size 3039761 ERR3317432 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR3317432 ERR3317432_1.fastq ERR3317432_2.fastq Input file: ERR3317432_1.fastq Paired file: ERR3317432_2.fastq trimmed: ERR3317432-trimmed-pair1.fastq, ERR3317432-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Dec 10 09:08:42 2024 >> started Tue Dec 10 09:08:55 2024 >> done (12.801s) 10626906 read pairs processed; of these: 159221 ( 1.50%) short read pairs filtered out after trimming by size control 125735 ( 1.18%) empty read pairs filtered out after trimming by size control 10341950 (97.32%) read pairs available; of these: 5115744 (49.47%) trimmed read pairs available after processing 5226206 (50.53%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1470 0.01% 19 2672 0.03% 20 2087 0.02% 21 2437 0.02% 22 4569 0.04% 23 4346 0.04% 24 1984 0.02% 25 1668 0.02% 26 2244 0.02% 27 3401 0.03% 28 3118 0.03% 29 2035 0.02% 30 1921 0.02% 31 2351 0.02% 32 2361 0.02% 33 2518 0.02% 34 2828 0.03% 35 3253 0.03% 36 3733 0.04% 37 4795 0.05% 38 43098 0.42% 39 5729 0.06% 40 4761 0.05% 41 4524 0.04% 42 5428 0.05% 43 5079 0.05% 44 5676 0.05% 45 6346 0.06% 46 6609 0.06% 47 10400 0.10% 48 8576 0.08% 49 8958 0.09% 50 8544 0.08% 51 9267 0.09% 52 8724 0.08% 53 9200 0.09% 54 9881 0.10% 55 11198 0.11% 56 12493 0.12% 57 12215 0.12% 58 13155 0.13% 59 13405 0.13% 60 14638 0.14% 61 14497 0.14% 62 15309 0.15% 63 16939 0.16% 64 17440 0.17% 65 19110 0.18% 66 19829 0.19% 67 20626 0.20% 68 21755 0.21% 69 24176 0.23% 70 24641 0.24% 71 28245 0.27% 72 28856 0.28% 73 29908 0.29% 74 31097 0.30% 75 30930 0.30% 76 32697 0.32% 77 34317 0.33% 78 38046 0.37% 79 41784 0.40% 80 38522 0.37% 81 38187 0.37% 82 40160 0.39% 83 39829 0.39% 84 43046 0.42% 85 48876 0.47% 86 51398 0.50% 87 54762 0.53% 88 53473 0.52% 89 84267 0.81% 90 52019 0.50% 91 50076 0.48% 92 47956 0.46% 93 47461 0.46% 94 58340 0.56% 95 50258 0.49% 96 52130 0.50% 97 59554 0.58% 98 52583 0.51% 99 51956 0.50% 100 52626 0.51% 101 55154 0.53% 102 55967 0.54% 103 57049 0.55% 104 58735 0.57% 105 62847 0.61% 106 69980 0.68% 107 66688 0.64% 108 74618 0.72% 109 107250 1.04% 110 77827 0.75% 111 76465 0.74% 112 77592 0.75% 113 76907 0.74% 114 79633 0.77% 115 82892 0.80% 116 85932 0.83% 117 89899 0.87% 118 95887 0.93% 119 104759 1.01% 120 122589 1.19% 121 144504 1.40% 122 186315 1.80% 123 289041 2.79% 124 1065838 10.31% 125 5226206 50.53% 10341950 reads passed initial QC criterion=sequence-density sequence-density=0.93 sequence-density-rank=1 fanout-score=48.81 fanout-score-rank=3 prefix-density=1.00 prefix-fanout=45.2 sequence=AGATCGGAAGAGCACAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=78.95 fanout-score-rank=1 prefix-density=0.09 prefix-fanout=6.0 sequence=AAGAAGGGCAGCATCGGCACCATCGCGCCGCTCGCGATTGGGTTCATTGTGGGAGCCAACATCCTTGTTGGTGGCGCGTTTGATGGCGCTTCCATGAACCCGGCTGTTTCCTTTGGCCCGGCGTTGGTTAGCTGGGAATGGGGGTACCAGTGGGTGTACTGGGTTGGCCCCCTCATCGGCGGTGGCCTTGCTGGGGTGATTTACGAGGTGCTCTTCATCTCCCACACCCATGAGCAGCTCCCCACCACCGACTACTAAGCAGATCGATGCAAGCTGCTTCTGCTTCCGAGTTCGTTCGGCCGGCGTCAATGCCCTCCGTTTCGTCGTCTCATCGTTTAGTTTCGTGAACCCATGTCCGACTGCTGCTGC criterion=sequence-density sequence-density=0.69 sequence-density-rank=1 fanout-score=29.26 fanout-score-rank=13 prefix-density=0.68 prefix-fanout=29.3 sequence=GGTGTGCTCTTCCGATCT criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=273.13 fanout-score-rank=1 prefix-density=0.17 prefix-fanout=9.6 sequence=CTTCTTCCTATTTAGTTTT ERR3317432 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 10 09:09:42 Started mapping on | Dec 10 09:09:42 Finished on | Dec 10 09:10:25 Mapping speed, Million of reads per hour | 865.84 Number of input reads | 10341950 Average input read length | 228 UNIQUE READS: Uniquely mapped reads number | 8508547 Uniquely mapped reads % | 82.27% Average mapped length | 229.50 Number of splices: Total | 3161558 Number of splices: Annotated (sjdb) | 2912510 Number of splices: GT/AG | 3102640 Number of splices: GC/AG | 50965 Number of splices: AT/AC | 2221 Number of splices: Non-canonical | 5732 Mismatch rate per base, % | 0.61% Deletion rate per base | 0.01% Deletion average length | 1.21 Insertion rate per base | 0.00% Insertion average length | 1.23 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1436756 % of reads mapped to multiple loci | 13.89% Number of reads mapped to too many loci | 29980 % of reads mapped to too many loci | 0.29% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.40% % of reads unmapped: other | 1.15% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 418750 418750 418750 N_multimapping 1436756 1436756 1436756 N_noFeature 540509 1116395 7635805 N_ambiguous 546545 319421 11124 UnstrandedReadsAssigned:7421493 PositiveStrandReadsAssigned:7072731 NegativeStrandReadsAssigned:861618 Dataset is classified positive stranded MeadianReadLen=125 20thPercentileLength=106 echo kmer=101 ERR3317432 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR3317432-trimmed-pair1.fastq ERR3317432-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 10,341,950 reads, 8,583,077 reads pseudoaligned [quant] estimated average fragment length: 168.582 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,083 rounds 52973 ERR3317432.ke.tsv 35125 ERR3317432.se.tsv 88098 total ==> ERR3317432.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 768.578 0 0 PNS24247 1044 876.418 0.640331 0.118414 PNS24249 1928 1760.42 0 0 PNS24246 1044 876.418 0.640331 0.118414 PNS24248 1044 876.418 0.640331 0.118414 PNS24244 1471 1303.42 208.079 25.8734 PNS24243 293 136.859 0 0 KQK14069 1603 1435.42 11407.7 1288.03 KQK14071 474 308.099 15.0379 7.91052 ==> ERR3317432.se.tsv <== BRADI_1g14170v3 11636 BRADI_1g53295v3 46 BRADI_1g59795v3 198 BRADI_1g07683v3 0 BRADI_1g00485v3 15 BRADI_1g20270v3 195 BRADI_1g74790v3 62 BRADI_1g09890v3 0 BRADI_1g77505v3 132 BRADI_1g48960v3 0 ERR3317432 completed mapping pipeline successfully