Starting /dee2/code/volunteer_pipeline.sh ERR3317437 current disk space = 1525206777856 free memory = 1595628016 ERR3317437 SRAfilesize 9ae852bb36dbda21e8ae8f102889fc4d ERR3317437.sra ERR3317437.sra file validated ERR3317437 is paired end ERR3317437 is conventional basespace ERR3317437 read1 length is 125 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR3317437_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.30575 33.0 33.0 33.0 27.0 33.0 2 31.43775 33.0 33.0 33.0 27.0 33.0 3 31.6815 33.0 33.0 33.0 27.0 33.0 4 31.579 33.0 33.0 33.0 27.0 33.0 5 31.715 33.0 33.0 33.0 33.0 33.0 6 33.82625 37.0 33.0 37.0 27.0 37.0 7 34.53325 37.0 37.0 37.0 27.0 37.0 8 34.777 37.0 37.0 37.0 27.0 37.0 9 34.862 37.0 37.0 37.0 27.0 37.0 10-11 35.180875 37.0 37.0 37.0 33.0 37.0 12-13 35.132875 37.0 37.0 37.0 33.0 37.0 14-15 35.052375 37.0 37.0 37.0 33.0 37.0 16-17 35.022125 37.0 37.0 37.0 33.0 37.0 18-19 35.205 37.0 37.0 37.0 33.0 37.0 20-21 34.766125 37.0 37.0 37.0 27.0 37.0 22-23 34.8955 37.0 37.0 37.0 27.0 37.0 24-25 35.10925 37.0 37.0 37.0 30.0 37.0 26-27 34.904875 37.0 37.0 37.0 30.0 37.0 28-29 34.934625 37.0 37.0 37.0 27.0 37.0 30-31 34.711124999999996 37.0 37.0 37.0 27.0 37.0 32-33 34.711375000000004 37.0 37.0 37.0 27.0 37.0 34-35 34.787 37.0 37.0 37.0 27.0 37.0 36-37 34.515874999999994 37.0 37.0 37.0 27.0 37.0 38-39 34.559875 37.0 37.0 37.0 27.0 37.0 40-41 34.131375 37.0 37.0 37.0 27.0 37.0 42-43 34.349875 37.0 37.0 37.0 27.0 37.0 44-45 34.299375 37.0 37.0 37.0 27.0 37.0 46-47 33.943 37.0 35.0 37.0 27.0 37.0 48-49 34.117374999999996 37.0 37.0 37.0 27.0 37.0 50-51 33.77575 37.0 35.0 37.0 27.0 37.0 52-53 34.06337499999999 37.0 37.0 37.0 27.0 37.0 54-55 34.058 37.0 37.0 37.0 27.0 37.0 56-57 33.6705 37.0 35.0 37.0 27.0 37.0 58-59 33.798249999999996 37.0 35.0 37.0 27.0 37.0 60-61 33.913 37.0 37.0 37.0 27.0 37.0 62-63 34.00625 37.0 37.0 37.0 27.0 37.0 64-65 34.077375 37.0 37.0 37.0 27.0 37.0 66-67 33.687749999999994 37.0 33.0 37.0 27.0 37.0 68-69 33.876999999999995 37.0 37.0 37.0 27.0 37.0 70-71 33.314875 37.0 33.0 37.0 20.5 37.0 72-73 33.16225 37.0 33.0 37.0 14.0 37.0 74-75 33.649874999999994 37.0 33.0 37.0 27.0 37.0 76-77 33.404624999999996 37.0 33.0 37.0 20.5 37.0 78-79 33.594375 37.0 35.0 37.0 24.5 37.0 80-81 33.539500000000004 37.0 35.0 37.0 24.5 37.0 82-83 33.184124999999995 37.0 35.0 37.0 18.0 37.0 84-85 33.009 37.0 33.0 37.0 14.0 37.0 86-87 32.937625 37.0 33.0 37.0 14.0 37.0 88-89 32.988625 37.0 33.0 37.0 14.0 37.0 90-91 32.950374999999994 37.0 33.0 37.0 14.0 37.0 92-93 33.055375 37.0 33.0 37.0 18.0 37.0 94-95 33.01975 37.0 33.0 37.0 14.0 37.0 96-97 32.771625 37.0 33.0 37.0 14.0 37.0 98-99 32.671375 37.0 33.0 37.0 14.0 37.0 100-101 32.8155 37.0 33.0 37.0 14.0 37.0 102-103 32.73625 37.0 33.0 37.0 14.0 37.0 104-105 32.64425 37.0 33.0 37.0 14.0 37.0 106-107 32.449875 37.0 33.0 37.0 14.0 37.0 108-109 32.336875 37.0 33.0 37.0 14.0 37.0 110-111 32.13249999999999 37.0 33.0 37.0 14.0 37.0 112-113 31.99275 37.0 33.0 37.0 14.0 37.0 114-115 31.566375 37.0 33.0 37.0 14.0 37.0 116-117 31.542749999999998 37.0 33.0 37.0 14.0 37.0 118-119 31.029875 37.0 33.0 37.0 8.0 37.0 120-121 30.688499999999998 37.0 33.0 37.0 2.0 37.0 122-123 29.75 37.0 30.0 37.0 2.0 37.0 124-125 25.777875 35.0 8.0 37.0 2.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 0.0 4 2.0 5 4.0 6 2.0 7 6.0 8 4.0 9 6.0 10 5.0 11 4.0 12 5.0 13 9.0 14 6.0 15 4.0 16 5.0 17 6.0 18 10.0 19 12.0 20 26.0 21 27.0 22 26.0 23 48.0 24 45.0 25 54.0 26 53.0 27 70.0 28 88.0 29 121.0 30 141.0 31 146.0 32 224.0 33 307.0 34 353.0 35 610.0 36 1565.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 22.380595148787197 25.95648912228057 22.48062015503876 29.182295573893473 2 24.3 23.925 20.625 31.15 3 25.85 25.7 21.7 26.75 4 27.6 23.150000000000002 21.075 28.175 5 29.275000000000002 22.625 19.55 28.549999999999997 6 28.266460905349795 27.109053497942387 19.650205761316872 24.97427983539095 7 26.6 23.9 24.0 25.5 8 28.275 25.724999999999998 26.6 19.400000000000002 9 28.549999999999997 27.275 26.85 17.325 10-11 28.537499999999998 27.0 24.637500000000003 19.825 12-13 28.4 26.55 25.4375 19.6125 14-15 25.825 26.75 25.637500000000003 21.7875 16-17 26.150000000000002 25.387500000000003 25.974999999999998 22.4875 18-19 25.575 25.162499999999998 26.5125 22.75 20-21 23.875 26.0 26.2625 23.8625 22-23 24.7 25.974999999999998 25.8125 23.5125 24-25 24.65 24.8625 28.1625 22.325 26-27 24.725 25.35 26.987499999999997 22.9375 28-29 25.8 25.337500000000002 25.4875 23.375 30-31 25.55 25.137500000000003 26.187500000000004 23.125 32-33 25.362499999999997 24.099999999999998 27.037499999999998 23.5 34-35 25.337500000000002 24.762500000000003 25.025 24.875 36-37 25.412499999999998 25.387500000000003 25.874999999999996 23.325000000000003 38-39 24.7875 25.2 26.1625 23.849999999999998 40-41 25.7125 24.8625 26.2125 23.2125 42-43 24.337500000000002 25.9625 26.3625 23.3375 44-45 25.4875 25.674999999999997 25.8625 22.975 46-47 26.150000000000002 24.8 25.5125 23.5375 48-49 24.224999999999998 25.1875 27.987499999999997 22.6 50-51 25.2875 24.6 26.224999999999998 23.8875 52-53 26.325 24.474999999999998 25.650000000000002 23.549999999999997 54-55 24.825 25.4625 27.487499999999997 22.225 56-57 25.7125 24.275 25.8125 24.2 58-59 25.35 24.325 26.187500000000004 24.1375 60-61 24.425 24.625 27.9375 23.0125 62-63 23.825 25.4625 27.6 23.1125 64-65 25.4 25.25 26.950000000000003 22.400000000000002 66-67 24.962500000000002 25.162499999999998 26.85 23.025000000000002 68-69 24.7875 26.5 26.424999999999997 22.287499999999998 70-71 25.7625 26.6625 25.25 22.325 72-73 25.025 26.8 25.4625 22.7125 74-75 25.074999999999996 26.6 25.95 22.375 76-77 25.124999999999996 27.500000000000004 24.6875 22.6875 78-79 24.7 27.500000000000004 25.5375 22.2625 80-81 24.9125 27.150000000000002 25.2625 22.675 82-83 24.9375 26.924999999999997 25.112499999999997 23.025000000000002 84-85 24.7 26.6 25.587500000000002 23.1125 86-87 24.975 26.775 24.375 23.875 88-89 26.200000000000003 26.575 23.974999999999998 23.25 90-91 25.025 27.2625 24.7375 22.975 92-93 25.5625 26.5375 24.75 23.150000000000002 94-95 25.4625 25.9875 25.25 23.3 96-97 24.9125 26.125 26.0 22.9625 98-99 25.5625 25.587500000000002 26.0 22.85 100-101 24.9875 26.2875 25.112499999999997 23.6125 102-103 24.65 26.625 25.174999999999997 23.549999999999997 104-105 25.087500000000002 27.2625 24.275 23.375 106-107 25.45 26.5125 24.5125 23.525 108-109 24.8 26.900000000000002 25.4625 22.8375 110-111 25.424999999999997 26.7125 24.65 23.2125 112-113 24.7 27.762500000000003 24.1375 23.400000000000002 114-115 25.3 26.937499999999996 24.4 23.3625 116-117 24.2625 28.000000000000004 24.525 23.2125 118-119 25.7125 27.0875 23.5625 23.6375 120-121 24.6625 27.55 24.5 23.2875 122-123 25.424999999999997 27.625 23.75 23.200000000000003 124-125 24.762500000000003 27.0875 23.7625 24.3875 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.0 2 1.0 3 1.5 4 1.5 5 0.5 6 0.5 7 1.0 8 0.5 9 0.0 10 0.5 11 1.0 12 0.5 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 1.5 22 2.0 23 3.0 24 4.0 25 2.0 26 4.5 27 8.5 28 9.0 29 8.0 30 7.5 31 11.0 32 15.5 33 19.0 34 22.0 35 31.5 36 46.0 37 63.0 38 80.5 39 101.5 40 134.0 41 161.0 42 177.5 43 196.5 44 215.5 45 221.0 46 211.0 47 219.5 48 212.5 49 179.5 50 168.0 51 154.5 52 123.5 53 106.5 54 99.5 55 88.0 56 84.0 57 74.5 58 70.5 59 63.0 60 57.0 61 53.5 62 51.0 63 49.5 64 42.5 65 40.5 66 35.0 67 37.5 68 35.5 69 29.5 70 30.5 71 24.5 72 20.5 73 18.0 74 14.5 75 12.0 76 10.5 77 9.5 78 7.0 79 4.0 80 1.5 81 1.0 82 1.0 83 1.5 84 0.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 2.8000000000000003 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.175 #Duplication Level Percentage of deduplicated Percentage of total 1 97.13685316522196 92.45 2 1.8912529550827424 3.5999999999999996 3 0.5516154452324665 1.575 4 0.07880220646178093 0.3 5 0.15760441292356187 0.75 6 0.10506960861570791 0.6 7 0.0 0.0 8 0.0 0.0 9 0.026267402153926978 0.22499999999999998 >10 0.052534804307853955 0.5 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG 10 0.25 TruSeq Adapter, Index 9 (100% over 49bp) ATGCCAGCTCTGACCGAAATCTTTGGGGATGATTCTGTATTACAATTTGG 10 0.25 No Hit GCAAAAAATTACGGTAGAGCGTGTTATGAGTGTCTACGTGGTGGACTTGA 9 0.22499999999999998 No Hit TGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTG 6 0.15 No Hit ATCCACGCCGGTACAGTAGTAGGTAAGTTAGAAGGGGAACGCGAAATCAC 6 0.15 No Hit TGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTG 6 0.15 No Hit CCACGCCGGTACAGTAGTAGGTAAGTTAGAAGGGGAACGCGAAATCACTT 6 0.15 No Hit ACTTAACTGGGGGATTCACCGCAAATACTACTTTGGCTCATTATTGCCGC 5 0.125 No Hit ATGCCAGGTGTTATACCAGTAGCTTCAGGTGGTATTCATGTTTGGCATAT 5 0.125 No Hit ATTTGAAGAGGGTTCCGTTACTAACATGTTTACTTCCATTGTAGGTAACG 5 0.125 No Hit ACGTGGTGGACTTGATTTTACCAAAGATGATGAAAACGTAAACTCACAAC 5 0.125 No Hit GTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACC 5 0.125 No Hit ACGCCGGTACAGTAGTAGGTAAGTTAGAAGGGGAACGCGAAATCACTTTA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.35 0.0 0.0 0.0 0.0 2 0.45 0.0 0.0 0.0 0.0 3 0.5 0.0 0.0 0.0 0.0 4 0.6 0.0 0.0 0.0 0.0 5 0.775 0.0 0.0 0.0 0.0 6 1.125 0.0 0.0 0.0 0.0 7 1.925 0.0 0.0 0.0 0.0 8 2.45 0.0 0.0 0.0 0.0 9 2.625 0.0 0.0 0.0 0.0 10-11 2.65 0.0 0.0 0.0 0.0 12-13 2.65 0.0 0.0 0.0 0.0 14-15 2.6875 0.0 0.0 0.0 0.0 16-17 2.7 0.0 0.0 0.0 0.0 18-19 2.725 0.0 0.0 0.0 0.0 20-21 2.75 0.0 0.0 0.0 0.0 22-23 2.775 0.0 0.0 0.0 0.0 24-25 2.775 0.0 0.0 0.0 0.0 26-27 2.8 0.0 0.0 0.0 0.0 28-29 2.8125 0.0 0.0 0.0 0.0 30-31 2.825 0.0 0.0 0.0 0.0 32-33 2.9124999999999996 0.0 0.0 0.0 0.0 34-35 2.925 0.0 0.0 0.0 0.0 36-37 3.0 0.0 0.0 0.0 0.0 38-39 3.1 0.0 0.0 0.0 0.0 40-41 3.2125 0.0 0.0 0.0 0.0 42-43 3.3125 0.0 0.0 0.0 0.0 44-45 3.425 0.0 0.0 0.0 0.0 46-47 3.5375 0.0 0.0 0.0 0.0 48-49 3.5875000000000004 0.0 0.0 0.0 0.0 50-51 3.675 0.0 0.0 0.0 0.0 52-53 3.825 0.0 0.0 0.0 0.0 54-55 3.9625 0.0 0.0 0.0 0.0 56-57 4.112500000000001 0.0 0.0 0.0 0.0 58-59 4.3375 0.0 0.0 0.0 0.0 60-61 4.5125 0.0 0.0 0.0 0.0 62-63 4.7 0.0 0.0 0.0 0.0 64-65 5.025 0.0 0.0 0.0 0.0 66-67 5.3375 0.0 0.0 0.0 0.0 68-69 5.675 0.0 0.0 0.0 0.0 70-71 5.9125 0.0 0.0 0.0 0.0 72-73 6.225 0.0 0.0 0.0 0.0 74-75 6.6625 0.0 0.0 0.0 0.0 76-77 7.0 0.0 0.0 0.0 0.0 78-79 7.449999999999999 0.0 0.0 0.0 0.0 80-81 8.1 0.0 0.0 0.0 0.0 82-83 8.5 0.0 0.0 0.0 0.0 84-85 8.9375 0.0 0.0 0.0 0.0 86-87 9.412500000000001 0.0 0.0 0.0 0.0 88-89 9.9375 0.0 0.0 0.0 0.0 90-91 10.6 0.0 0.0 0.0 0.0 92-93 11.0 0.0 0.0 0.0 0.0 94-95 11.575 0.0 0.0 0.0 0.0 96-97 11.9875 0.0 0.0 0.0 0.0 98-99 12.625 0.0 0.0 0.0 0.0 100-101 13.2375 0.0 0.0 0.0 0.0 102-103 13.875 0.0 0.0 0.0 0.0 104-105 14.475000000000001 0.0 0.0 0.0 0.0 106-107 15.25 0.0 0.0 0.0 0.0 108-109 15.9375 0.0 0.0 0.0 0.0 110-111 16.65 0.0 0.0 0.0 0.0 112-113 17.35 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE ERR3317437 read2 length is 125 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR3317437_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.28725 33.0 33.0 33.0 14.0 33.0 2 30.64675 33.0 33.0 33.0 27.0 33.0 3 30.54975 33.0 33.0 33.0 27.0 33.0 4 30.206 33.0 33.0 33.0 14.0 33.0 5 30.48675 33.0 33.0 33.0 27.0 33.0 6 33.89225 37.0 37.0 37.0 27.0 37.0 7 33.417 37.0 33.0 37.0 14.0 37.0 8 33.19475 37.0 33.0 37.0 14.0 37.0 9 33.30725 37.0 33.0 37.0 14.0 37.0 10-11 33.730625 37.0 35.0 37.0 27.0 37.0 12-13 33.534625000000005 37.0 37.0 37.0 20.5 37.0 14-15 33.590875 37.0 37.0 37.0 20.5 37.0 16-17 33.657125 37.0 37.0 37.0 27.0 37.0 18-19 33.061875 37.0 33.0 37.0 14.0 37.0 20-21 33.07475 37.0 33.0 37.0 14.0 37.0 22-23 33.216 37.0 33.0 37.0 14.0 37.0 24-25 33.223875 37.0 35.0 37.0 18.0 37.0 26-27 33.140875 37.0 35.0 37.0 18.0 37.0 28-29 33.118375 37.0 35.0 37.0 18.0 37.0 30-31 33.026375 37.0 33.0 37.0 14.0 37.0 32-33 33.368875 37.0 37.0 37.0 24.5 37.0 34-35 33.28375 37.0 35.0 37.0 14.0 37.0 36-37 33.02075 37.0 33.0 37.0 14.0 37.0 38-39 33.381125 37.0 37.0 37.0 24.5 37.0 40-41 33.111875 37.0 33.0 37.0 14.0 37.0 42-43 33.31975 37.0 37.0 37.0 18.0 37.0 44-45 33.118375 37.0 35.0 37.0 14.0 37.0 46-47 33.100750000000005 37.0 35.0 37.0 18.0 37.0 48-49 33.24275 37.0 37.0 37.0 14.0 37.0 50-51 33.027625 37.0 35.0 37.0 14.0 37.0 52-53 33.16375 37.0 37.0 37.0 14.0 37.0 54-55 33.086125 37.0 35.0 37.0 14.0 37.0 56-57 33.245375 37.0 35.0 37.0 22.0 37.0 58-59 32.97825 37.0 35.0 37.0 14.0 37.0 60-61 32.84225 37.0 33.0 37.0 14.0 37.0 62-63 32.47325 37.0 33.0 37.0 14.0 37.0 64-65 32.67225 37.0 33.0 37.0 14.0 37.0 66-67 32.423625 37.0 33.0 37.0 14.0 37.0 68-69 32.544125 37.0 33.0 37.0 14.0 37.0 70-71 32.535875000000004 37.0 33.0 37.0 14.0 37.0 72-73 32.249375 37.0 33.0 37.0 14.0 37.0 74-75 31.7435 37.0 33.0 37.0 14.0 37.0 76-77 32.006125 37.0 33.0 37.0 14.0 37.0 78-79 31.874625 37.0 33.0 37.0 14.0 37.0 80-81 31.74325 37.0 33.0 37.0 14.0 37.0 82-83 31.7085 37.0 33.0 37.0 14.0 37.0 84-85 31.732625 37.0 33.0 37.0 14.0 37.0 86-87 31.842624999999998 37.0 33.0 37.0 14.0 37.0 88-89 31.357 37.0 33.0 37.0 14.0 37.0 90-91 31.728499999999997 37.0 33.0 37.0 14.0 37.0 92-93 31.678874999999998 37.0 33.0 37.0 14.0 37.0 94-95 31.814 37.0 33.0 37.0 14.0 37.0 96-97 31.711 37.0 33.0 37.0 14.0 37.0 98-99 31.721125 37.0 33.0 37.0 14.0 37.0 100-101 31.7535 37.0 33.0 37.0 14.0 37.0 102-103 31.585625 37.0 33.0 37.0 14.0 37.0 104-105 31.48575 37.0 33.0 37.0 14.0 37.0 106-107 31.381 37.0 33.0 37.0 14.0 37.0 108-109 31.280250000000002 37.0 33.0 37.0 14.0 37.0 110-111 30.96575 37.0 33.0 37.0 14.0 37.0 112-113 31.08125 37.0 33.0 37.0 8.0 37.0 114-115 30.877000000000002 37.0 33.0 37.0 2.0 37.0 116-117 30.519375 37.0 33.0 37.0 2.0 37.0 118-119 30.577625 37.0 33.0 37.0 2.0 37.0 120-121 30.22775 37.0 33.0 37.0 2.0 37.0 122-123 29.494625 37.0 30.0 37.0 2.0 37.0 124-125 27.304875 37.0 14.5 37.0 2.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 77.0 3 21.0 4 5.0 5 12.0 6 6.0 7 4.0 8 8.0 9 5.0 10 9.0 11 8.0 12 6.0 13 5.0 14 8.0 15 3.0 16 11.0 17 15.0 18 18.0 19 17.0 20 26.0 21 38.0 22 59.0 23 49.0 24 63.0 25 57.0 26 71.0 27 62.0 28 99.0 29 118.0 30 155.0 31 147.0 32 185.0 33 203.0 34 321.0 35 434.0 36 1675.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 31.624999999999996 11.95 27.224999999999998 29.2 2 26.8 12.45 22.55 38.2 3 22.925 15.125 26.05 35.9 4 25.374999999999996 13.325000000000001 25.85 35.449999999999996 5 26.1 15.575 22.3 36.025 6 29.299999999999997 18.775 27.05 24.875 7 21.125 27.175 34.875 16.825000000000003 8 18.95 29.4 32.324999999999996 19.325 9 18.775 30.675 31.424999999999997 19.125 10-11 20.7 27.8375 31.05 20.4125 12-13 22.4375 28.237499999999997 28.249999999999996 21.075 14-15 20.9875 27.575 27.987499999999997 23.45 16-17 21.9 28.787499999999998 26.437500000000004 22.875 18-19 23.2375 27.287499999999998 26.75 22.725 20-21 21.175 27.8375 26.424999999999997 24.5625 22-23 22.15 26.187500000000004 27.3 24.3625 24-25 21.1625 28.462500000000002 27.3 23.075000000000003 26-27 20.9125 27.825 25.0625 26.200000000000003 28-29 22.3125 27.5625 24.3 25.825 30-31 22.45 28.5875 26.3625 22.6 32-33 22.625 29.275000000000002 24.4125 23.6875 34-35 22.287499999999998 28.3125 24.925 24.474999999999998 36-37 21.6875 26.937499999999996 27.6625 23.7125 38-39 22.525000000000002 27.375 25.275 24.825 40-41 23.025000000000002 25.874999999999996 25.224999999999998 25.874999999999996 42-43 22.5125 26.825 25.4875 25.174999999999997 44-45 23.45 26.625 24.425 25.5 46-47 23.325000000000003 25.587500000000002 24.2625 26.825 48-49 23.2625 27.6375 25.412499999999998 23.6875 50-51 23.8125 25.5375 25.4875 25.162499999999998 52-53 23.175 25.3 26.1 25.424999999999997 54-55 21.0625 29.012500000000003 26.05 23.875 56-57 22.5125 25.7875 26.487500000000004 25.2125 58-59 22.575 26.4125 26.174999999999997 24.837500000000002 60-61 19.8625 28.9125 26.450000000000003 24.775 62-63 22.0625 28.3625 24.5625 25.0125 64-65 22.2 27.150000000000002 25.074999999999996 25.575 66-67 21.725 30.7 24.325 23.25 68-69 21.725 29.8375 24.5125 23.925 70-71 21.275 29.7125 24.2 24.8125 72-73 22.7375 29.6375 23.825 23.799999999999997 74-75 22.3875 28.262500000000003 24.212500000000002 25.137500000000003 76-77 24.1375 27.762500000000003 23.4125 24.6875 78-79 23.2875 28.825 23.2625 24.625 80-81 23.0 27.2625 24.6 25.137500000000003 82-83 23.5125 26.674999999999997 23.9 25.912499999999998 84-85 24.875 27.487499999999997 24.1625 23.474999999999998 86-87 25.525 26.687499999999996 23.962500000000002 23.825 88-89 23.962500000000002 26.375 24.462500000000002 25.2 90-91 24.6 29.312500000000004 24.0125 22.075 92-93 24.975 26.85 24.349999999999998 23.825 94-95 24.762500000000003 26.0375 24.8625 24.337500000000002 96-97 25.074999999999996 27.3 24.7875 22.8375 98-99 24.325 26.974999999999998 23.3625 25.337500000000002 100-101 24.2 26.75 24.2875 24.762500000000003 102-103 25.6125 28.1 23.8625 22.425 104-105 24.775 27.187499999999996 24.525 23.5125 106-107 24.45 26.1625 24.25 25.137500000000003 108-109 25.124999999999996 26.937499999999996 23.8875 24.05 110-111 25.0375 28.425 22.5 24.0375 112-113 24.75 26.5875 24.025 24.637500000000003 114-115 25.4625 27.212500000000002 23.7375 23.5875 116-117 25.55 26.650000000000002 23.6875 24.1125 118-119 26.125 26.787499999999998 23.75 23.3375 120-121 24.4875 27.8625 25.05 22.6 122-123 25.337500000000002 29.9875 22.912499999999998 21.762500000000003 124-125 25.650000000000002 27.375 24.099999999999998 22.875 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5.0 1 4.0 2 2.0 3 0.5 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.5 21 2.0 22 1.5 23 2.5 24 3.5 25 5.0 26 6.5 27 6.0 28 5.5 29 11.0 30 17.5 31 21.0 32 26.5 33 31.5 34 41.0 35 49.0 36 47.0 37 65.0 38 100.5 39 121.5 40 151.0 41 182.0 42 211.5 43 219.0 44 206.5 45 210.5 46 211.0 47 201.0 48 187.5 49 174.5 50 159.5 51 145.0 52 122.0 53 102.0 54 84.0 55 73.5 56 81.5 57 78.0 58 59.0 59 52.5 60 50.0 61 49.5 62 52.5 63 43.0 64 38.0 65 34.5 66 33.5 67 34.5 68 29.0 69 24.5 70 23.0 71 19.5 72 16.5 73 15.5 74 10.0 75 7.0 76 8.0 77 6.0 78 3.5 79 3.5 80 4.0 81 3.0 82 1.5 83 1.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.72500000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 98.11320754716981 94.89999999999999 2 1.4990953734815198 2.9000000000000004 3 0.15507883173946757 0.44999999999999996 4 0.07753941586973379 0.3 5 0.025846471956577927 0.125 6 0.0 0.0 7 0.025846471956577927 0.17500000000000002 8 0.051692943913155855 0.4 9 0.0 0.0 >10 0.051692943913155855 0.75 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCACCAAATTGTAATACAGAATCATCCCCAAAGATTTCGGTCAGAGCTGG 16 0.4 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 14 0.35000000000000003 Illumina Single End PCR Primer 1 (100% over 50bp) TCTTCATATTTAGTTTTATCTATTAATCGATAGTATCTACCGGCGCGAAC 8 0.2 No Hit CCACCGAATTGTAATACAGAATCATCCCCAAAGATTTCGGTCAGAGCTGG 8 0.2 No Hit TCTTTATATTTAGTTTTATCTATTAATCGATAGTATCTACCGGCGCGAAC 7 0.17500000000000002 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.35 0.0 0.0 0.0 0.0 2 0.475 0.0 0.0 0.0 0.0 3 0.525 0.0 0.0 0.0 0.0 4 0.625 0.0 0.0 0.0 0.0 5 0.85 0.0 0.0 0.0 0.0 6 1.2 0.0 0.0 0.0 0.0 7 2.075 0.0 0.0 0.0 0.0 8 2.6 0.0 0.0 0.0 0.0 9 2.775 0.0 0.0 0.0 0.0 10-11 2.8 0.0 0.0 0.0 0.0 12-13 2.8 0.0 0.0 0.0 0.0 14-15 2.8375000000000004 0.0 0.0 0.0 0.0 16-17 2.85 0.0 0.0 0.0 0.0 18-19 2.85 0.0 0.0 0.0 0.0 20-21 2.875 0.0 0.0 0.0 0.0 22-23 2.9 0.0 0.0 0.0 0.0 24-25 2.9 0.0 0.0 0.0 0.0 26-27 2.925 0.0 0.0 0.0 0.0 28-29 2.9375 0.0 0.0 0.0 0.0 30-31 2.95 0.0 0.0 0.0 0.0 32-33 3.0125 0.0 0.0 0.0 0.0 34-35 3.025 0.0 0.0 0.0 0.0 36-37 3.0875000000000004 0.0 0.0 0.0 0.0 38-39 3.175 0.0 0.0 0.0 0.0 40-41 3.3125 0.0 0.0 0.0 0.0 42-43 3.425 0.0 0.0 0.0 0.0 44-45 3.5 0.0 0.0 0.0 0.0 46-47 3.6125 0.0 0.0 0.0 0.0 48-49 3.6624999999999996 0.0 0.0 0.0 0.0 50-51 3.7750000000000004 0.0 0.0 0.0 0.0 52-53 3.95 0.0 0.0 0.0 0.0 54-55 4.1375 0.0 0.0 0.0 0.0 56-57 4.3125 0.0 0.0 0.0 0.0 58-59 4.5375 0.0 0.0 0.0 0.0 60-61 4.775 0.0 0.0 0.0 0.0 62-63 4.9625 0.0 0.0 0.0 0.0 64-65 5.275 0.0 0.0 0.0 0.0 66-67 5.5875 0.0 0.0 0.0 0.0 68-69 5.9 0.0 0.0 0.0 0.0 70-71 6.175000000000001 0.0 0.0 0.0 0.0 72-73 6.512499999999999 0.0 0.0 0.0 0.0 74-75 6.9625 0.0 0.0 0.0 0.0 76-77 7.2875 0.0 0.0 0.0 0.0 78-79 7.75 0.0 0.0 0.0 0.0 80-81 8.375 0.0 0.0 0.0 0.0 82-83 8.775 0.0 0.0 0.0 0.0 84-85 9.1875 0.0 0.0 0.0 0.0 86-87 9.725 0.0 0.0 0.0 0.0 88-89 10.225000000000001 0.0 0.0 0.0 0.0 90-91 10.9 0.0 0.0 0.0 0.0 92-93 11.3625 0.0 0.0 0.0 0.0 94-95 11.925 0.0 0.0 0.0 0.0 96-97 12.3625 0.0 0.0 0.0 0.0 98-99 13.05 0.0 0.0 0.0 0.0 100-101 13.6375 0.0 0.0 0.0 0.0 102-103 14.3375 0.0 0.0 0.0 0.0 104-105 14.95 0.0 0.0 0.0 0.0 106-107 15.675 0.0 0.0 0.0 0.0 108-109 16.35 0.0 0.0 0.0 0.0 110-111 17.049999999999997 0.0 0.0 0.0 0.0 112-113 17.8 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGAAT 15 0.0040846216 59.5 50-51 CGCCTTC 15 0.0040846216 59.5 62-63 AATTTGA 15 0.0040846216 59.5 54-55 GGCGCGA 15 0.0040846216 59.5 42-43 ATCTATT 15 0.0040846216 59.5 18-19 GCGGCTA 15 0.0040846216 59.5 84-85 TGATCGC 15 0.0040846216 59.5 58-59 ACTCCAT 15 0.0040846216 59.5 98-99 ACTTCAC 15 0.0040846216 59.5 72-73 AACTCGA 15 0.0040846216 59.5 48-49 CTGCGGC 15 0.0040846216 59.5 82-83 TACCGGC 15 0.0040846216 59.5 38-39 ATTAATC 15 0.0040846216 59.5 22-23 CGAATTT 15 0.0040846216 59.5 52-53 TTCCATA 15 0.0040846216 59.5 66-67 CCTTCCA 15 0.0040846216 59.5 64-65 TAATCGA 15 0.0040846216 59.5 24-25 CTAGTTC 15 0.0040846216 59.5 88-89 CTGCTCG 15 0.0040846216 59.5 112-113 ATACTTC 15 0.0040846216 59.5 70-71 >>END_MODULE Read 5522431 spots for ERR3317437.sra Written 5522431 spots for ERR3317437.sra Read 5522431 spots for ERR3317437.sra Written 5522431 spots for ERR3317437.sra Read 5522431 spots for ERR3317437.sra Written 5522431 spots for ERR3317437.sra Read 5522431 spots for ERR3317437.sra Written 5522431 spots for ERR3317437.sra Read 5522431 spots for ERR3317437.sra Written 5522431 spots for ERR3317437.sra Read 5522431 spots for ERR3317437.sra Written 5522431 spots for ERR3317437.sra Read 5522431 spots for ERR3317437.sra Written 5522431 spots for ERR3317437.sra Read 5522431 spots for ERR3317437.sra Written 5522431 spots for ERR3317437.sra Read 5522431 spots for ERR3317437.sra Written 5522431 spots for ERR3317437.sra Read 5522431 spots for ERR3317437.sra Written 5522431 spots for ERR3317437.sra Read 5522431 spots for ERR3317437.sra Written 5522431 spots for ERR3317437.sra Read 5522431 spots for ERR3317437.sra Written 5522431 spots for ERR3317437.sra Read 5522431 spots for ERR3317437.sra Written 5522431 spots for ERR3317437.sra Read 5522431 spots for ERR3317437.sra Written 5522431 spots for ERR3317437.sra Read 5522431 spots for ERR3317437.sra Written 5522431 spots for ERR3317437.sra Read 5522431 spots for ERR3317437.sra Written 5522431 spots for ERR3317437.sra Read 5522431 spots for ERR3317437.sra Written 5522431 spots for ERR3317437.sra Read 5522445 spots for ERR3317437.sra Written 5522445 spots for ERR3317437.sra Read 5522431 spots for ERR3317437.sra Written 5522431 spots for ERR3317437.sra Read 5522431 spots for ERR3317437.sra Written 5522431 spots for ERR3317437.sra SRR ids: ['ERR3317437.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_jgbt8rh7 ERR3317437.sra spots: 110448634 blocks: [[1, 5522431], [5522432, 11044862], [11044863, 16567293], [16567294, 22089724], [22089725, 27612155], [27612156, 33134586], [33134587, 38657017], [38657018, 44179448], [44179449, 49701879], [49701880, 55224310], [55224311, 60746741], [60746742, 66269172], [66269173, 71791603], [71791604, 77314034], [77314035, 82836465], [82836466, 88358896], [88358897, 93881327], [93881328, 99403758], [99403759, 104926189], [104926190, 110448634]] ERR3317437 file size 31817405 ERR3317437 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR3317437 ERR3317437_1.fastq ERR3317437_2.fastq Input file: ERR3317437_1.fastq Paired file: ERR3317437_2.fastq trimmed: ERR3317437-trimmed-pair1.fastq, ERR3317437-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Dec 10 09:31:01 2024 >> started Tue Dec 10 09:32:52 2024 >> done (110.983s) 110448634 read pairs processed; of these: 4118786 ( 3.73%) short read pairs filtered out after trimming by size control 1773093 ( 1.61%) empty read pairs filtered out after trimming by size control 104556755 (94.67%) read pairs available; of these: 50078864 (47.90%) trimmed read pairs available after processing 54477891 (52.10%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4493 0.00% 19 13142 0.01% 20 13314 0.01% 21 23209 0.02% 22 10974 0.01% 23 11878 0.01% 24 8774 0.01% 25 9826 0.01% 26 11146 0.01% 27 12668 0.01% 28 12839 0.01% 29 13328 0.01% 30 14243 0.01% 31 42813 0.04% 32 21365 0.02% 33 23262 0.02% 34 29966 0.03% 35 34860 0.03% 36 28485 0.03% 37 28238 0.03% 38 35641 0.03% 39 37822 0.04% 40 40539 0.04% 41 43672 0.04% 42 53400 0.05% 43 50538 0.05% 44 55568 0.05% 45 57299 0.05% 46 57747 0.06% 47 68360 0.07% 48 70809 0.07% 49 72087 0.07% 50 81017 0.08% 51 83431 0.08% 52 84557 0.08% 53 98846 0.09% 54 106113 0.10% 55 109197 0.10% 56 110509 0.11% 57 114344 0.11% 58 117108 0.11% 59 124687 0.12% 60 148697 0.14% 61 141429 0.14% 62 146530 0.14% 63 154591 0.15% 64 177836 0.17% 65 165568 0.16% 66 185297 0.18% 67 187679 0.18% 68 192241 0.18% 69 211317 0.20% 70 217463 0.21% 71 259953 0.25% 72 276230 0.26% 73 297274 0.28% 74 300527 0.29% 75 300449 0.29% 76 308566 0.30% 77 327744 0.31% 78 336888 0.32% 79 327510 0.31% 80 325938 0.31% 81 315377 0.30% 82 323308 0.31% 83 338014 0.32% 84 346052 0.33% 85 379472 0.36% 86 385653 0.37% 87 380143 0.36% 88 369788 0.35% 89 446453 0.43% 90 371321 0.36% 91 366795 0.35% 92 390371 0.37% 93 368423 0.35% 94 391121 0.37% 95 389392 0.37% 96 411491 0.39% 97 403956 0.39% 98 401592 0.38% 99 427687 0.41% 100 425804 0.41% 101 490359 0.47% 102 461606 0.44% 103 466434 0.45% 104 472492 0.45% 105 504667 0.48% 106 500371 0.48% 107 509836 0.49% 108 513557 0.49% 109 588063 0.56% 110 553558 0.53% 111 578035 0.55% 112 623590 0.60% 113 623354 0.60% 114 647011 0.62% 115 680018 0.65% 116 740858 0.71% 117 811999 0.78% 118 903318 0.86% 119 1086369 1.04% 120 1319098 1.26% 121 1684712 1.61% 122 2506559 2.40% 123 4345116 4.16% 124 13803830 13.20% 125 54477891 52.10% 104556755 reads passed initial QC criterion=sequence-density sequence-density=0.54 sequence-density-rank=1 fanout-score=52.61 fanout-score-rank=4 prefix-density=0.59 prefix-fanout=48.4 sequence=AGATCGGAAGAGCACACC criterion=fanout-score sequence-density=0.09 sequence-density-rank=14 fanout-score=108.50 fanout-score-rank=1 prefix-density=0.57 prefix-fanout=17.3 sequence=GCCGCCGCCGCCA criterion=sequence-density sequence-density=1.04 sequence-density-rank=1 fanout-score=31.14 fanout-score-rank=7 prefix-density=1.04 prefix-fanout=31.0 sequence=GGTGTGCTCTTCCGATCT criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=109.25 fanout-score-rank=1 prefix-density=0.13 prefix-fanout=5.3 sequence=TTTTCCTTTTTGTTTGTTTCGAGAAAGATATCGTCCGATTCTCCTTCTATTGATTCTTTTCCGATCGAGATGTATGGATCCATGTGTCTACATACCTAGATTCTGTTCATGGATTAACGAAAATGTGCAAGAGCTCTATTTGCCTCTGCCATTCTATGAGTCGCTTCCTTTTTGCGTATGGCACCCCCACTCCCTTTGGCAGCATCTACTAATTCGGAACTTAATTTGAAAGCCATATTTCGACCCGGACGCTTTTGGGATGCTTCTAATAACCAACGAATGGCAAGTGCTCTTCCTTGTTTAGATCCTATTTCAATCGGAACTTTCCGCGTCGATCCTTTTTTATTACGTCTTGTTTTTACTCCTATATTGGGAGTTACTCTACGTATTGCTTGACGTAAAACCAATAGTGGATTTGTTTCTGTCTTTTGTTGAATCTTTTTGACGGCTCGATAGAGAATTTGATAAGCCAATGATTTTTTTCCGTCTTTCATA ERR3317437 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 10 09:33:38 Started mapping on | Dec 10 09:33:38 Finished on | Dec 10 09:39:13 Mapping speed, Million of reads per hour | 1123.59 Number of input reads | 104556755 Average input read length | 233 UNIQUE READS: Uniquely mapped reads number | 85638929 Uniquely mapped reads % | 81.91% Average mapped length | 232.50 Number of splices: Total | 57967252 Number of splices: Annotated (sjdb) | 54616677 Number of splices: GT/AG | 57104451 Number of splices: GC/AG | 761395 Number of splices: AT/AC | 34795 Number of splices: Non-canonical | 66611 Mismatch rate per base, % | 0.63% Deletion rate per base | 0.01% Deletion average length | 1.26 Insertion rate per base | 0.00% Insertion average length | 1.34 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 16207875 % of reads mapped to multiple loci | 15.50% Number of reads mapped to too many loci | 240354 % of reads mapped to too many loci | 0.23% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 0.80% % of reads unmapped: other | 1.56% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2953367 2953367 2953367 N_multimapping 16207875 16207875 16207875 N_noFeature 4271743 10615030 77504132 N_ambiguous 3232700 1545835 209918 UnstrandedReadsAssigned:78134486 PositiveStrandReadsAssigned:73478064 NegativeStrandReadsAssigned:7924879 Dataset is classified positive stranded MeadianReadLen=125 20thPercentileLength=116 echo kmer=111 ERR3317437 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR3317437-trimmed-pair1.fastq ERR3317437-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 104,556,755 reads, 87,660,831 reads pseudoaligned [quant] estimated average fragment length: 194.337 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,325 rounds 52973 ERR3317437.ke.tsv 35125 ERR3317437.se.tsv 88098 total ==> ERR3317437.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 742.991 0 0 PNS24247 1044 850.663 170.536 3.28875 PNS24249 1928 1734.66 280.011 2.6481 PNS24246 1044 850.663 170.536 3.28875 PNS24248 1044 850.663 170.536 3.28875 PNS24244 1471 1277.66 686.382 8.81298 PNS24243 293 123.738 0 0 KQK14069 1603 1409.66 70760.3 823.471 KQK14071 474 285.156 144.036 8.28633 ==> ERR3317437.se.tsv <== BRADI_1g14170v3 75975 BRADI_1g53295v3 566 BRADI_1g59795v3 1680 BRADI_1g07683v3 0 BRADI_1g00485v3 173 BRADI_1g20270v3 5706 BRADI_1g74790v3 1314 BRADI_1g09890v3 8 BRADI_1g77505v3 1237 BRADI_1g48960v3 0 ERR3317437 completed mapping pipeline successfully