Starting /dee2/code/volunteer_pipeline.sh ERR5262779 current disk space = 1551485022208 free memory = 1368070464 ERR5262779 SRAfilesize 48601d025840a4d6003b93933ab16279 ERR5262779.sra ERR5262779.sra file validated ERR5262779 is paired end ERR5262779 is conventional basespace ERR5262779 read1 length is 66-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262779_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 66-150 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6095 37.0 37.0 37.0 37.0 37.0 2 36.58875 37.0 37.0 37.0 37.0 37.0 3 36.677 37.0 37.0 37.0 37.0 37.0 4 36.6725 37.0 37.0 37.0 37.0 37.0 5 36.7145 37.0 37.0 37.0 37.0 37.0 6 36.4445 37.0 37.0 37.0 37.0 37.0 7 36.541 37.0 37.0 37.0 37.0 37.0 8 36.73 37.0 37.0 37.0 37.0 37.0 9 36.7885 37.0 37.0 37.0 37.0 37.0 10-14 36.716499999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.5773 37.0 37.0 37.0 37.0 37.0 20-24 36.6836 37.0 37.0 37.0 37.0 37.0 25-29 36.6499 37.0 37.0 37.0 37.0 37.0 30-34 36.786699999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.6726 37.0 37.0 37.0 37.0 37.0 40-44 36.611599999999996 37.0 37.0 37.0 37.0 37.0 45-49 36.791900000000005 37.0 37.0 37.0 37.0 37.0 50-54 36.678200000000004 37.0 37.0 37.0 37.0 37.0 55-59 36.7952 37.0 37.0 37.0 37.0 37.0 60-64 36.75 37.0 37.0 37.0 37.0 37.0 65-69 36.56505056264066 37.0 37.0 37.0 37.0 37.0 70-74 36.70202550637659 37.0 37.0 37.0 37.0 37.0 75-79 36.629407351837955 37.0 37.0 37.0 37.0 37.0 80-84 36.6418560369837 37.0 37.0 37.0 37.0 37.0 85-89 36.59238906837042 37.0 37.0 37.0 37.0 37.0 90-94 36.589321451426784 37.0 37.0 37.0 37.0 37.0 95-99 36.58811515128697 37.0 37.0 37.0 37.0 37.0 100-104 36.64544652671951 37.0 37.0 37.0 37.0 37.0 105-109 36.591230394725955 37.0 37.0 37.0 37.0 37.0 110-114 36.51119461476684 37.0 37.0 37.0 37.0 37.0 115-119 36.66845386759356 37.0 37.0 37.0 37.0 37.0 120-124 36.6192468320732 37.0 37.0 37.0 37.0 37.0 125-129 36.6676408189588 37.0 37.0 37.0 37.0 37.0 130-134 36.61271841778057 37.0 37.0 37.0 37.0 37.0 135-139 36.30837992181939 37.0 37.0 37.0 37.0 37.0 140-144 36.46960005264059 37.0 37.0 37.0 37.0 37.0 145-149 36.33106741816995 37.0 37.0 37.0 37.0 37.0 150 36.372603500972495 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 27 1.0 28 3.0 29 2.0 30 19.0 31 12.0 32 17.0 33 39.0 34 68.0 35 131.0 36 2932.0 37 776.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 64.925 17.724999999999998 1.4749999999999999 15.875 2 50.23767825869402 1.0007505629221916 34.701025769326996 14.060545409056793 3 31.474999999999998 29.175 24.075 15.275 4 10.7 44.6 21.099999999999998 23.599999999999998 5 10.825 42.55 46.0 0.625 6 28.825 31.15 39.375 0.65 7 30.0 8.75 25.95 35.3 8 20.0 1.275 24.8 53.925 9 31.25 38.725 30.0 0.025 10-14 29.75 26.13 28.645 15.475 15-19 16.0 47.089999999999996 14.13 22.78 20-24 20.005 44.905 15.1 19.99 25-29 29.98 49.345 2.915 17.76 30-34 21.235 40.635 5.87 32.26 35-39 38.255 33.995 10.005 17.745 40-44 23.73 40.005 12.005 24.26 45-49 30.14 39.87 2.0 27.99 50-54 14.64 57.46 2.01 25.89 55-59 8.01 35.730000000000004 12.015 44.245000000000005 60-64 34.01 34.905 19.475 11.61 65-69 10.006500975146272 38.07571135670351 15.772365854878231 36.14542181327199 70-74 11.222805701425356 35.90897724431108 28.997249312328083 23.870967741935484 75-79 4.801200300075019 47.301825456364085 17.76444111027757 30.132533133283324 80-84 0.015012760846719713 42.906470499924936 17.164589901416207 39.91392683781214 85-89 9.181676784907932 33.59590587526968 20.927198835984147 36.29521850383824 90-94 24.83561712593485 27.96767555087085 13.863373989860964 33.33333333333333 95-99 34.3396795097202 23.31843070276787 21.93198372431808 20.40990606319385 100-104 29.914788483840066 38.8544345283114 10.069076791206575 21.16170019664196 105-109 20.234351222481486 32.48960129856954 26.260525514862533 21.015521964086435 110-114 16.408175842235003 32.02547247329498 21.975143796220216 29.591207888249794 115-119 42.1555957611786 15.988627552339105 14.174205220987336 27.681571465494958 120-124 46.82947062788407 30.797946803546427 11.785140249909265 10.587442318660237 125-129 20.429544264012574 43.41016238868517 14.436877946568885 21.723415400733366 130-134 13.596607474158496 34.455340577789556 19.671349059104163 32.27670288894779 135-139 16.535007033870794 57.55870576777406 18.477437506763337 7.428849691591818 140-144 34.827172609311965 32.49918220477592 19.71431686838949 12.959328317522626 145-149 24.195148842337378 55.154355016538034 4.448732083792724 16.201764057331864 150 49.5415393164768 26.67407613225896 14.78188385662684 9.0025006946374 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 2.5 40 2.5 41 4.0 42 323.0 43 554.5 44 474.5 45 239.0 46 1.0 47 2.5 48 162.0 49 198.5 50 1020.0 51 999.5 52 17.5 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.075 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 66-67 1.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.0 82-83 8.0 84-85 4.0 86-87 1.0 88-89 0.0 90-91 0.0 92-93 1.0 94-95 3.0 96-97 0.0 98-99 7.0 100-101 10.0 102-103 2.0 104-105 8.0 106-107 26.0 108-109 21.0 110-111 14.0 112-113 8.0 114-115 10.0 116-117 12.0 118-119 4.0 120-121 1.0 122-123 2.0 124-125 34.0 126-127 13.0 128-129 11.0 130-131 11.0 132-133 65.0 134-135 21.0 136-137 15.0 138-139 4.0 140-141 11.0 142-143 13.0 144-145 8.0 146-147 47.0 148-149 4.0 150-151 3599.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 1.375 #Duplication Level Percentage of deduplicated Percentage of total 1 41.81818181818181 0.575 2 12.727272727272727 0.35000000000000003 3 9.090909090909092 0.375 4 3.6363636363636362 0.2 5 1.8181818181818181 0.125 6 1.8181818181818181 0.15 7 0.0 0.0 8 0.0 0.0 9 1.8181818181818181 0.22499999999999998 >10 16.363636363636363 4.925 >50 0.0 0.0 >100 5.454545454545454 28.875 >500 3.6363636363636362 34.849999999999994 >1k 1.8181818181818181 29.349999999999998 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGATAGCGAACGGAACAGAACAGGAACACACGACAGGTAGCATCACGGA 1174 29.349999999999998 No Hit GGATAGCGAACGGAACAGAACAGGAACACACGACAGGTAGCATCACGGAC 798 19.950000000000003 No Hit CTCATTCTTTTTTCATTCATTCATAGGGATAGCGAACGGAACAGAACAGG 596 14.899999999999999 No Hit GCTCATTCTTTTTTCATTCATTCATAGGGATAGCGAACGGAACAGAACAG 461 11.525 No Hit ATTCATTCATAGGGATAGCGAACGGAACAGAACAGGAACACACGACAGGT 400 10.0 No Hit ATAGGGATAGCGAACGGAACAGAACAGGAACACACGACAGGTAGCATCAC 294 7.35 No Hit GTAGGGATAGCGAACGGAACAGAACAGGAACACACGACAGGTAGCATCAC 39 0.975 No Hit GCTCGTTCTTTTTTCATTCATTCATAGGGATAGCGAACGGAACAGAACAG 33 0.8250000000000001 No Hit TAGGGATAGCGAACGGAACAGAACAGGAACACACGACAGGTAGCATCACG 24 0.6 No Hit TCATTCTTTTTTCATTCATTCATAGGGATAGCGAACGGAACAGAACAGGA 22 0.5499999999999999 No Hit GGGGTAGCGAACGGAACAGAACAGGAACACACGACAGGTAGCATCACGGA 21 0.525 No Hit GCTCCTTCTTTTTTCATTCATTCATAGGGATAGCGAACGGAACAGAACAG 20 0.5 No Hit CAGGGATAGCGAACGGAACAGAACAGGAACACACGACAGGTAGCATCACG 13 0.325 No Hit GCTTATTCTTTTTTCATTCATTCATAGGGATAGCGAACGGAACAGAACAG 13 0.325 No Hit GTTCATTCTTTTTTCATTCATTCATAGGGATAGCGAACGGAACAGAACAG 12 0.3 No Hit ATGGGGATAGCGAACGGAACAGAACAGGAACACACGACAGGTAGCATCAC 9 0.22499999999999998 No Hit CTCGTTCTTTTTTCATTCATTCATAGGGATAGCGAACGGAACAGAACAGG 6 0.15 No Hit TTTTTTTTTTTTTCATTCATTCATAGGGATAGCGAACGGAACAGAACAGG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTCATT 45 0.0 141.99998 1 CTCATTC 105 0.0 81.14286 1 CATTCTT 110 0.0 77.454544 3 TCATTCT 110 0.0 77.454544 2 ATTCTTT 115 0.0 74.08696 4 TTTTTTC 120 0.0 70.99999 8 TTTTTCA 120 0.0 70.99999 9 TTCTTTT 120 0.0 70.99999 5 TCTTTTT 120 0.0 70.99999 6 CTTTTTT 120 0.0 70.99999 7 GGGATAG 315 0.0 51.84127 1 TAGCGAA 395 0.0 43.13924 5 AGCGAAC 395 0.0 43.13924 6 GAACGGA 400 0.0 42.600002 9 GCGAACG 400 0.0 42.600002 7 CGAACGG 400 0.0 42.600002 8 GGATAGC 390 0.0 41.871796 2 ATAGCGA 390 0.0 41.871796 4 GATAGCG 390 0.0 41.871796 3 TTCATAG 160 0.0 35.5 6 >>END_MODULE ERR5262779 read2 length is 66-150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR5262779_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 66-150 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.189 37.0 37.0 37.0 37.0 37.0 2 36.3185 37.0 37.0 37.0 37.0 37.0 3 36.3845 37.0 37.0 37.0 37.0 37.0 4 36.5255 37.0 37.0 37.0 37.0 37.0 5 36.3115 37.0 37.0 37.0 37.0 37.0 6 36.4885 37.0 37.0 37.0 37.0 37.0 7 36.537 37.0 37.0 37.0 37.0 37.0 8 36.5055 37.0 37.0 37.0 37.0 37.0 9 36.4425 37.0 37.0 37.0 37.0 37.0 10-14 36.5453 37.0 37.0 37.0 37.0 37.0 15-19 36.472 37.0 37.0 37.0 37.0 37.0 20-24 36.4773 37.0 37.0 37.0 37.0 37.0 25-29 36.4702 37.0 37.0 37.0 37.0 37.0 30-34 36.3943 37.0 37.0 37.0 37.0 37.0 35-39 36.402699999999996 37.0 37.0 37.0 37.0 37.0 40-44 36.385000000000005 37.0 37.0 37.0 37.0 37.0 45-49 36.3093 37.0 37.0 37.0 37.0 37.0 50-54 36.328199999999995 37.0 37.0 37.0 37.0 37.0 55-59 36.2241 37.0 37.0 37.0 37.0 37.0 60-64 36.2361 37.0 37.0 37.0 37.0 37.0 65-69 36.32169882470618 37.0 37.0 37.0 37.0 37.0 70-74 36.12788197049262 37.0 37.0 37.0 37.0 37.0 75-79 36.23560890222556 37.0 37.0 37.0 37.0 37.0 80-84 35.96201585554945 37.0 37.0 37.0 37.0 37.0 85-89 35.99120651653248 37.0 37.0 37.0 37.0 37.0 90-94 35.99582351992663 37.0 37.0 37.0 37.0 37.0 95-99 35.959807449513136 37.0 37.0 37.0 37.0 37.0 100-104 35.97094749197815 37.0 37.0 37.0 37.0 37.0 105-109 35.957385564497756 37.0 37.0 37.0 37.0 37.0 110-114 35.90650146425229 37.0 37.0 37.0 37.0 37.0 115-119 35.988020407557926 37.0 37.0 37.0 37.0 37.0 120-124 35.781990994929146 37.0 37.0 37.0 37.0 37.0 125-129 35.80054261399816 37.0 37.0 37.0 37.0 37.0 130-134 35.79660980144991 37.0 37.0 37.0 37.0 37.0 135-139 35.747331155491466 37.0 37.0 37.0 37.0 37.0 140-144 35.7441240028611 37.0 37.0 37.0 37.0 37.0 145-149 35.55625303864844 37.0 37.0 37.0 37.0 37.0 150 35.62086230876217 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 1.0 13 1.0 14 0.0 15 1.0 16 0.0 17 2.0 18 1.0 19 2.0 20 0.0 21 1.0 22 2.0 23 4.0 24 1.0 25 5.0 26 9.0 27 5.0 28 9.0 29 17.0 30 14.0 31 15.0 32 38.0 33 51.0 34 122.0 35 492.0 36 2987.0 37 219.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 80.025 4.75 4.775 10.45 2 31.1 7.875 38.9 22.125 3 43.325 8.0 24.775 23.9 4 46.6 32.125 9.725 11.55 5 29.5 19.525000000000002 19.725 31.25 6 46.025 9.45 17.224999999999998 27.3 7 54.1 6.550000000000001 25.35 14.000000000000002 8 32.074999999999996 8.799999999999999 20.575 38.550000000000004 9 31.35 10.975 32.375 25.3 10-14 43.285000000000004 11.459999999999999 19.175 26.08 15-19 35.115 12.740000000000002 22.61 29.535 20-24 38.35 13.77 25.169999999999998 22.71 25-29 37.97 13.04 25.424999999999997 23.565 30-34 31.330000000000002 14.64 27.834999999999997 26.195 35-39 31.819999999999997 13.465 30.285 24.43 40-44 32.06 13.035 29.909999999999997 24.995 45-49 31.135 14.899999999999999 31.264999999999997 22.7 50-54 31.814999999999998 15.65 31.435000000000002 21.099999999999998 55-59 28.595 14.48 31.895 25.03 60-64 30.04 15.2 29.73 25.03 65-69 29.76446466970046 15.647347102065309 28.614292143821572 25.97389608441266 70-74 23.815953988497125 15.893973493373343 33.833458364591145 26.456614153538382 75-79 29.397349337334333 17.694423605901473 30.782695673918482 22.12553138284571 80-84 26.582595205925035 14.317169594155033 34.904668968623334 24.195566231296603 85-89 27.529978425568206 16.65746826551603 30.906627866138177 24.905925442777583 90-94 27.872308387291074 15.017818601616224 32.08854088239723 25.021332128695477 95-99 29.587017684887464 18.23251607717042 32.74216237942122 19.4383038585209 100-104 28.7270526528142 15.886624974783134 36.54428081500908 18.842041557393586 105-109 26.727549467275498 17.447995941146626 36.23033992897007 19.594114662607815 110-114 26.258475446887196 17.325868091226628 35.19108280254777 21.224573659338404 115-119 26.094598087361078 15.766347893512537 34.784181959162574 23.354872059963817 120-124 24.74723907295069 15.564888266708147 36.65681547156115 23.031057188780007 125-129 30.42430591932949 14.520691461498167 35.40597171293871 19.64903090623363 130-134 23.62576199310893 14.852902199840976 40.51417969785317 21.007156109196927 135-139 26.45817552212964 16.432204306893194 35.07196190888432 22.037658262092847 140-144 25.54410080183276 15.343915343915343 37.13522064037528 21.976763213876616 145-149 25.24555788544311 14.601037413089063 37.2972078137071 22.85619688776073 150 25.81363004172462 15.32684283727399 31.710709318497916 27.148817802503476 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.5 32 1.0 33 0.5 34 0.5 35 0.5 36 0.0 37 0.0 38 0.5 39 1.5 40 2.0 41 5.0 42 15.5 43 36.0 44 134.0 45 197.0 46 114.0 47 82.0 48 170.5 49 229.0 50 124.5 51 54.5 52 361.0 53 481.0 54 226.5 55 78.5 56 27.5 57 82.0 58 119.5 59 161.0 60 170.0 61 92.5 62 46.5 63 19.0 64 14.0 65 60.5 66 59.0 67 35.0 68 47.5 69 60.5 70 60.0 71 25.5 72 23.0 73 18.0 74 8.5 75 53.5 76 160.0 77 211.0 78 103.5 79 10.0 80 8.0 81 3.5 82 1.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 66-67 1.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.0 82-83 8.0 84-85 4.0 86-87 1.0 88-89 0.0 90-91 0.0 92-93 1.0 94-95 3.0 96-97 1.0 98-99 7.0 100-101 10.0 102-103 2.0 104-105 8.0 106-107 26.0 108-109 20.0 110-111 15.0 112-113 8.0 114-115 9.0 116-117 12.0 118-119 4.0 120-121 1.0 122-123 2.0 124-125 34.0 126-127 13.0 128-129 11.0 130-131 11.0 132-133 65.0 134-135 21.0 136-137 15.0 138-139 5.0 140-141 12.0 142-143 13.0 144-145 10.0 146-147 47.0 148-149 4.0 150-151 3595.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 8.85 #Duplication Level Percentage of deduplicated Percentage of total 1 43.78531073446328 3.875 2 11.016949152542372 1.95 3 5.932203389830509 1.575 4 4.80225988700565 1.7000000000000002 5 3.672316384180791 1.625 6 2.824858757062147 1.5 7 1.4124293785310735 0.8750000000000001 8 1.977401129943503 1.4000000000000001 9 0.2824858757062147 0.22499999999999998 >10 18.64406779661017 38.125 >50 4.23728813559322 27.3 >100 1.4124293785310735 19.85 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTGAATGGTGGGGCCCCTCGTGGCCAGTTATCCTTGTTACCTTTTATCTG 270 6.75 No Hit GTGATGTTATCGCTCCCGAGTATCCTAGATCTCGCTCCATCGCGTAGGGT 150 3.75 No Hit GTTATCCTTGTTACCTTTTATCTGTGATGTTATCGCTCCCGAGTATCCTA 147 3.675 No Hit GTTACCTTTTATCTGTGATGTTATCGCTCCCGAGTATCCTAGATCTCGCT 117 2.9250000000000003 No Hit GCTCCGGCGGCGGCAACTGGAGGGAGTGAATGGTGGGGCCCCTCGTGGCC 110 2.75 No Hit GGTGGGGCCCCTCGTGGCCAGTTATCCTTGTTACCTTTTATCTGTGATGT 99 2.475 No Hit GTGGAGGCTCCGGCGGCGGCAACTGGAGGGAGTGAATGGTGGGGCCCCTC 94 2.35 No Hit GTGGGGCCCCTCGTGGCCAGTTATCCTTGTTACCTTTTATCTGTGATGTT 89 2.225 No Hit CTCGTGGCCAGTTATCCTTGTTACCTTTTATCTGTGATGTTATCGCTCCC 86 2.15 No Hit GAATGGTGGGGCCCCTCGTGGCCAGTTATCCTTGTTACCTTTTATCTGTG 80 2.0 No Hit GGCAACTGGAGGGAGTGAATGGTGGGGCCCCTCGTGGCCAGTTATCCTTG 79 1.975 No Hit GCGGCGGCTACGGTGGCAGCCGTGGAGGCTCCGGCGGCGGCAACTGGAGG 72 1.7999999999999998 No Hit GGCCCCTCGTGGCCAGTTATCCTTGTTACCTTTTATCTGTGATGTTATCG 71 1.775 No Hit GTGGCAGCCGTGGAGGCTCCGGCGGCGGCAACTGGAGGGAGTGAATGGTG 68 1.7000000000000002 No Hit GCCGTGGAGGCTCCGGCGGCGGCAACTGGAGGGAGTGAATGGTGGGGCCC 67 1.675 No Hit GGCTCCGGCGGCGGCAACTGGAGGGAGTGAATGGTGGGGCCCCTCGTGGC 65 1.625 No Hit GGGAGTGAATGGTGGGGCCCCTCGTGGCCAGTTATCCTTGTTACCTTTTA 57 1.425 No Hit GTGGCGGCGGCGGCTACGGTGGTGGCGGCGGCGGCTACGGTGGCAGCCGT 56 1.4000000000000001 No Hit GCGGCGGCGGCTACGGTGGCAGCCGTGGAGGCTCCGGCGGCGGCAACTGG 56 1.4000000000000001 No Hit GTCAAGGTGGCGGCGGCTATGGTGGCGGCGGCGGCTACGGTGGTGGCGGC 53 1.325 No Hit GGGGCCCCTCGTGGCCAGTTATCCTTGTTACCTTTTATCTGTGATGTTAT 47 1.175 No Hit GTGGCGGCGGCGGCTACGGTGGCAGCCGTGGAGGCTCCGGCGGCGGCAAC 46 1.15 No Hit CTGGAGGGAGTGAATGGTGGGGCCCCTCGTGGCCAGTTATCCTTGTTACC 45 1.125 No Hit GCTACGGTGGCAGCCGTGGAGGCTCCGGCGGCGGCAACTGGAGGGAGTGA 43 1.075 No Hit GCGGCAACTGGAGGGAGTGAATGGTGGGGCCCCTCGTGGCCAGTTATCCT 42 1.05 No Hit GCGGCGGCAACTGGAGGGAGTGAATGGTGGGGCCCCTCGTGGCCAGTTAT 42 1.05 No Hit AGTGAATGGTGGGGCCCCTCGTGGCCAGTTATCCTTGTTACCTTTTATCT 42 1.05 No Hit GGCAGCCGTGGAGGCTCCGGCGGCGGCAACTGGAGGGAGTGAATGGTGGG 41 1.0250000000000001 No Hit CCTAGATCTCGCTCCATCGCGTAGGGTTTGAGATGTTTAAGGGTTACCAT 41 1.0250000000000001 No Hit TATCGCTCCCGAGTATCCTAGATCTCGCTCCATCGCGTAGGGTTTGAGAT 41 1.0250000000000001 No Hit GCCAGTTATCCTTGTTACCTTTTATCTGTGATGTTATCGCTCCCGAGTAT 37 0.9249999999999999 No Hit GGCGGCGGCGGCTACGGTGGCAGCCGTGGAGGCTCCGGCGGCGGCAACTG 36 0.8999999999999999 No Hit GCGGCGGCGGCTACGGTGGTGGCGGCGGCGGCTACGGTGGCAGCCGTGGA 36 0.8999999999999999 No Hit GGCGGCGGCTACGGTGGCAGCCGTGGAGGCTCCGGCGGCGGCAACTGGAG 34 0.8500000000000001 No Hit GTGGTGGCGGCGGCGGCTACGGTGGCAGCCGTGGAGGCTCCGGCGGCGGC 33 0.8250000000000001 No Hit GTGGCGGCGGCTATGGTGGCGGCGGCGGCTACGGTGGTGGCGGCGGCGGC 33 0.8250000000000001 No Hit GGCGGCGGCTATGGTGGCGGCGGCGGCTACGGTGGTGGCGGCGGCGGCTA 31 0.775 No Hit GGAGTGAATGGTGGGGCCCCTCGTGGCCAGTTATCCTTGTTACCTTTTAT 31 0.775 No Hit GAGTGAATGGTGGGGCCCCTCGTGGCCAGTTATCCTTGTTACCTTTTATC 30 0.75 No Hit GAGGCTCCGGCGGCGGCAACTGGAGGGAGTGAATGGTGGGGCCCCTCGTG 28 0.7000000000000001 No Hit GGAGGCTCCGGCGGCGGCAACTGGAGGGAGTGAATGGTGGGGCCCCTCGT 27 0.675 No Hit TGGTGGGGCCCCTCGTGGCCAGTTATCCTTGTTACCTTTTATCTGTGATG 27 0.675 No Hit GAGATGTTTAAGGGTTACCATTAGGTGTTTGTCCGTGATGCTACCTGTCG 26 0.65 No Hit GGCGGCGGCAACTGGAGGGAGTGAATGGTGGGGCCCCTCGTGGCCAGTTA 24 0.6 No Hit GCAACTGGAGGGAGTGAATGGTGGGGCCCCTCGTGGCCAGTTATCCTTGT 24 0.6 No Hit ATCGCTCCCGAGTATCCTAGATCTCGCTCCATCGCGTAGGGTTTGAGATG 23 0.575 No Hit GCTACGGTGGTGGCGGCGGCGGCTACGGTGGCAGCCGTGGAGGCTCCGGC 23 0.575 No Hit GGCGGCGGCGGCTACGGTGGTGGCGGCGGCGGCTACGGTGGCAGCCGTGG 23 0.575 No Hit GCGGCGGCTACGGTGGTGGCGGCGGCGGCTACGGTGGCAGCCGTGGAGGC 22 0.5499999999999999 No Hit GGCCAGTTATCCTTGTTACCTTTTATCTGTGATGTTATCGCTCCCGAGTA 22 0.5499999999999999 No Hit GCCCCTCGTGGCCAGTTATCCTTGTTACCTTTTATCTGTGATGTTATCGC 22 0.5499999999999999 No Hit CCTTGTTACCTTTTATCTGTGATGTTATCGCTCCCGAGTATCCTAGATCT 21 0.525 No Hit GCTATGGCCAGCGTCAAGGTGGCGGCGGCTATGGTGGCGGCGGCGGCTAC 21 0.525 No Hit GTTATCGCTCCCGAGTATCCTAGATCTCGCTCCATCGCGTAGGGTTTGAG 20 0.5 No Hit GGAGGGAGTGAATGGTGGGGCCCCTCGTGGCCAGTTATCCTTGTTACCTT 20 0.5 No Hit GGCGGCGGCTACGGTGGTGGCGGCGGCGGCTACGGTGGCAGCCGTGGAGG 20 0.5 No Hit GGTGGCGGCGGCGGCTACGGTGGCAGCCGTGGAGGCTCCGGCGGCGGCAA 19 0.475 No Hit GGCTACGGTGGCAGCCGTGGAGGCTCCGGCGGCGGCAACTGGAGGGAGTG 19 0.475 No Hit GGTGGCAGCCGTGGAGGCTCCGGCGGCGGCAACTGGAGGGAGTGAATGGT 19 0.475 No Hit GAGGGAGTGAATGGTGGGGCCCCTCGTGGCCAGTTATCCTTGTTACCTTT 18 0.44999999999999996 No Hit GGTGGTGGCGGCGGCGGCTACGGTGGCAGCCGTGGAGGCTCCGGCGGCGG 17 0.42500000000000004 No Hit TGGGGCCCCTCGTGGCCAGTTATCCTTGTTACCTTTTATCTGTGATGTTA 17 0.42500000000000004 No Hit CTACGGTGGCAGCCGTGGAGGCTCCGGCGGCGGCAACTGGAGGGAGTGAA 15 0.375 No Hit GCTATGGTGGCGGCGGCGGCTACGGTGGTGGCGGCGGCGGCTACGGTGGC 15 0.375 No Hit GGTGGCGGCGGCGGCTACGGTGGTGGCGGCGGCGGCTACGGTGGCAGCCG 15 0.375 No Hit GCCAGCGTCAAGGTGGCGGCGGCTATGGTGGCGGCGGCGGCTACGGTGGT 14 0.35000000000000003 No Hit GCGGCGGCTATGGTGGCGGCGGCGGCTACGGTGGTGGCGGCGGCGGCTAC 14 0.35000000000000003 No Hit CAGCCGTGGAGGCTCCGGCGGCGGCAACTGGAGGGAGTGAATGGTGGGGC 14 0.35000000000000003 No Hit CTTGTTACCTTTTATCTGTGATGTTATCGCTCCCGAGTATCCTAGATCTC 14 0.35000000000000003 No Hit GTGGCCAGTTATCCTTGTTACCTTTTATCTGTGATGTTATCGCTCCCGAG 13 0.325 No Hit GGCGGCAACTGGAGGGAGTGAATGGTGGGGCCCCTCGTGGCCAGTTATCC 13 0.325 No Hit TGGCAGCCGTGGAGGCTCCGGCGGCGGCAACTGGAGGGAGTGAATGGTGG 12 0.3 No Hit CGAGTATCCTAGATCTCGCTCCATCGCGTAGGGTTTGAGATGTTTAAGGG 12 0.3 No Hit ATCCTAGATCTCGCTCCATCGCGTAGGGTTTGAGATGTTTAAGGGTTACC 12 0.3 No Hit GCGGCTACGGTGGCAGCCGTGGAGGCTCCGGCGGCGGCAACTGGAGGGAG 12 0.3 No Hit GGCGGCTATGGTGGCGGCGGCGGCTACGGTGGTGGCGGCGGCGGCTACGG 11 0.27499999999999997 No Hit TATCCTTGTTACCTTTTATCTGTGATGTTATCGCTCCCGAGTATCCTAGA 11 0.27499999999999997 No Hit AGGGAGTGAATGGTGGGGCCCCTCGTGGCCAGTTATCCTTGTTACCTTTT 11 0.27499999999999997 No Hit CGGCAACTGGAGGGAGTGAATGGTGGGGCCCCTCGTGGCCAGTTATCCTT 11 0.27499999999999997 No Hit CTTTTATCTGTGATGTTATCGCTCCCGAGTATCCTAGATCTCGCTCCATC 11 0.27499999999999997 No Hit CTGTGATGTTATCGCTCCCGAGTATCCTAGATCTCGCTCCATCGCGTAGG 11 0.27499999999999997 No Hit ACTGGAGGGAGTGAATGGTGGGGCCCCTCGTGGCCAGTTATCCTTGTTAC 11 0.27499999999999997 No Hit TGAATGGTGGGGCCCCTCGTGGCCAGTTATCCTTGTTACCTTTTATCTGT 10 0.25 No Hit GATGTTATCGCTCCCGAGTATCCTAGATCTCGCTCCATCGCGTAGGGTTT 10 0.25 No Hit GTATCCTAGATCTCGCTCCATCGCGTAGGGTTTGAGATGTTTAAGGGTTA 10 0.25 No Hit TGTTATCGCTCCCGAGTATCCTAGATCTCGCTCCATCGCGTAGGGTTTGA 10 0.25 No Hit GGCGGCTACGGTGGCAGCCGTGGAGGCTCCGGCGGCGGCAACTGGAGGGA 9 0.22499999999999998 No Hit GCGTCAAGGTGGCGGCGGCTATGGTGGCGGCGGCGGCTACGGTGGTGGCG 8 0.2 No Hit GGTGTTTGTCCGTGATGCTACCTGTCGTGTGTTCCTGTTCTGTTCCGTTC 8 0.2 No Hit ATCCTTGTTACCTTTTATCTGTGATGTTATCGCTCCCGAGTATCCTAGAT 8 0.2 No Hit GGCCAGCGTCAAGGTGGCGGCGGCTATGGTGGCGGCGGCGGCTACGGTGG 8 0.2 No Hit GCGGCGGTGGCGGCTATGGCCAGCGTCAAGGTGGCGGCGGCTATGGTGGC 8 0.2 No Hit GCGGCTATGGCCAGCGTCAAGGTGGCGGCGGCTATGGTGGCGGCGGCGGC 8 0.2 No Hit GGCGGCTACGGTGGTGGCGGCGGCGGCTACGGTGGCAGCCGTGGAGGCTC 8 0.2 No Hit CTACGGTGGTGGCGGCGGCGGCTACGGTGGCAGCCGTGGAGGCTCCGGCG 7 0.17500000000000002 No Hit TTAAGGGTTACCATTAGGTGTTTGTCCGTGATGCTACCTGTCGTGTGTTC 7 0.17500000000000002 No Hit GGCGGCTATGGCCAGCGTCAAGGTGGCGGCGGCTATGGTGGCGGCGGCGG 7 0.17500000000000002 No Hit AGTTATCCTTGTTACCTTTTATCTGTGATGTTATCGCTCCCGAGTATCCT 7 0.17500000000000002 No Hit GGCTATGGTGGCGGCGGCGGCTACGGTGGTGGCGGCGGCGGCTACGGTGG 7 0.17500000000000002 No Hit GGTGGCGGCGGCTATGGTGGCGGCGGCGGCTACGGTGGTGGCGGCGGCGG 6 0.15 No Hit CAACTGGAGGGAGTGAATGGTGGGGCCCCTCGTGGCCAGTTATCCTTGTT 6 0.15 No Hit GCGGTGGCGGCTATGGCCAGCGTCAAGGTGGCGGCGGCTATGGTGGCGGC 6 0.15 No Hit CTAGATCTCGCTCCATCGCGTAGGGTTTGAGATGTTTAAGGGTTACCATT 6 0.15 No Hit CCGGCGGCGGCAACTGGAGGGAGTGAATGGTGGGGCCCCTCGTGGCCAGT 6 0.15 No Hit GTGGCGGCGGTGGCGGCTATGGCCAGCGTCAAGGTGGCGGCGGCTATGGT 6 0.15 No Hit GTGGTGGCGGCGGTGGCGGCTATGGCCAGCGTCAAGGTGGCGGCGGCTAT 6 0.15 No Hit GTTTAAGGGTTACCATTAGGTGTTTGTCCGTGATGCTACCTGTCGTGTGT 6 0.15 No Hit CGTAGGGTTTGAGATGTTTAAGGGTTACCATTAGGTGTTTGTCCGTGATG 6 0.15 No Hit TGGAGGGAGTGAATGGTGGGGCCCCTCGTGGCCAGTTATCCTTGTTACCT 6 0.15 No Hit CGGCTATGGCCAGCGTCAAGGTGGCGGCGGCTATGGTGGCGGCGGCGGCT 5 0.125 No Hit CGGCGGTGGCGGCTATGGCCAGCGTCAAGGTGGCGGCGGCTATGGTGGCG 5 0.125 No Hit GCAGCCGTGGAGGCTCCGGCGGCGGCAACTGGAGGGAGTGAATGGTGGGG 5 0.125 No Hit AAGGTGGCGGCGGCTATGGTGGCGGCGGCGGCTACGGTGGTGGCGGCGGC 5 0.125 No Hit GCGGCTATGGTGGCGGCGGCGGCTACGGTGGTGGCGGCGGCGGCTACGGT 5 0.125 No Hit GGCTACGGTGGTGGCGGCGGCGGCTACGGTGGCAGCCGTGGAGGCTCCGG 5 0.125 No Hit ATGGTGGGGCCCCTCGTGGCCAGTTATCCTTGTTACCTTTTATCTGTGAT 5 0.125 No Hit GATGTTTAAGGGTTACCATTAGGTGTTTGTCCGTGATGCTACCTGTCGTG 5 0.125 No Hit AGTATCCTAGATCTCGCTCCATCGCGTAGGGTTTGAGATGTTTAAGGGTT 5 0.125 No Hit CGGCGGCTACGGTGGCAGCCGTGGAGGCTCCGGCGGCGGCAACTGGAGGG 5 0.125 No Hit ATGGTGGCGGCGGCGGCTACGGTGGTGGCGGCGGCGGCTACGGTGGCAGC 5 0.125 No Hit CTCCGGCGGCGGCAACTGGAGGGAGTGAATGGTGGGGCCCCTCGTGGCCA 5 0.125 No Hit TGGAGGCTCCGGCGGCGGCAACTGGAGGGAGTGAATGGTGGGGCCCCTCG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTATCC 70 9.89667E-6 54.826252 140-144 GTTCGCT 75 9.741907E-4 25.585587 135-139 GAATGGT 265 0.0018590987 16.07547 3 AATGGTG 265 0.0018590987 16.07547 4 TGAATGG 265 0.0018590987 16.07547 2 TGGTGGG 265 0.0018590987 16.07547 6 GTGAATG 270 0.0021071115 15.777778 1 TGGGGCC 275 0.0023824838 15.490909 9 GTGGGGC 280 0.0026876163 15.214286 8 GGTGGGG 280 0.0026876163 15.214286 7 GCTACCT 160 0.004159158 14.489797 140-144 ATGGTGG 320 0.0065432293 13.3125 5 CTACCTG 160 0.0044207615 11.347033 140-144 CGGCGGC 825 0.004462502 7.745455 5 >>END_MODULE Read 1578088 spots for ERR5262779.sra Written 1578088 spots for ERR5262779.sra Read 1578088 spots for ERR5262779.sra Written 1578088 spots for ERR5262779.sra Read 1578088 spots for ERR5262779.sra Written 1578088 spots for ERR5262779.sra Read 1578088 spots for ERR5262779.sra Written 1578088 spots for ERR5262779.sra Read 1578088 spots for ERR5262779.sra Written 1578088 spots for ERR5262779.sra Read 1578088 spots for ERR5262779.sra Written 1578088 spots for ERR5262779.sra Read 1578088 spots for ERR5262779.sra Written 1578088 spots for ERR5262779.sra Read 1578088 spots for ERR5262779.sra Written 1578088 spots for ERR5262779.sra Read 1578088 spots for ERR5262779.sra Written 1578088 spots for ERR5262779.sra Read 1578088 spots for ERR5262779.sra Written 1578088 spots for ERR5262779.sra Read 1578088 spots for ERR5262779.sra Written 1578088 spots for ERR5262779.sra Read 1578088 spots for ERR5262779.sra Written 1578088 spots for ERR5262779.sra Read 1578088 spots for ERR5262779.sra Written 1578088 spots for ERR5262779.sra Read 1578088 spots for ERR5262779.sra Written 1578088 spots for ERR5262779.sra Read 1578088 spots for ERR5262779.sra Written 1578088 spots for ERR5262779.sra Read 1578088 spots for ERR5262779.sra Written 1578088 spots for ERR5262779.sra Read 1578088 spots for ERR5262779.sra Written 1578088 spots for ERR5262779.sra Read 1578088 spots for ERR5262779.sra Written 1578088 spots for ERR5262779.sra Read 1578088 spots for ERR5262779.sra Written 1578088 spots for ERR5262779.sra Read 1578088 spots for ERR5262779.sra Written 1578088 spots for ERR5262779.sra SRR ids: ['ERR5262779.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_xm1xuxrz ERR5262779.sra spots: 31561760 blocks: [[1, 1578088], [1578089, 3156176], [3156177, 4734264], [4734265, 6312352], [6312353, 7890440], [7890441, 9468528], [9468529, 11046616], [11046617, 12624704], [12624705, 14202792], [14202793, 15780880], [15780881, 17358968], [17358969, 18937056], [18937057, 20515144], [20515145, 22093232], [22093233, 23671320], [23671321, 25249408], [25249409, 26827496], [26827497, 28405584], [28405585, 29983672], [29983673, 31561760]] ERR5262779 file size 10395131 ERR5262779 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR5262779 ERR5262779_1.fastq ERR5262779_2.fastq Input file: ERR5262779_1.fastq Paired file: ERR5262779_2.fastq trimmed: ERR5262779-trimmed-pair1.fastq, ERR5262779-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:11:45 2024 >> started Fri Dec 6 11:12:20 2024 >> done (35.574s) 31561760 read pairs processed; of these: 0 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 31561760 (100.00%) read pairs available; of these: 8435 ( 0.03%) trimmed read pairs available after processing 31553325 (99.97%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 23 2 0.00% 24 3 0.00% 25 1 0.00% 26 1 0.00% 27 3 0.00% 28 1 0.00% 29 2 0.00% 30 5 0.00% 31 7 0.00% 32 2 0.00% 33 2 0.00% 34 5 0.00% 35 7 0.00% 36 3 0.00% 37 8 0.00% 38 5 0.00% 39 7 0.00% 40 1 0.00% 41 1 0.00% 42 4 0.00% 43 2 0.00% 44 3 0.00% 45 6 0.00% 46 6 0.00% 47 3 0.00% 48 2 0.00% 49 327 0.00% 50 317 0.00% 51 391 0.00% 52 403 0.00% 53 443 0.00% 54 462 0.00% 55 504 0.00% 56 570 0.00% 57 536 0.00% 58 718 0.00% 59 745 0.00% 60 849 0.00% 61 854 0.00% 62 1065 0.00% 63 1161 0.00% 64 1305 0.00% 65 1382 0.00% 66 1464 0.00% 67 1762 0.01% 68 1932 0.01% 69 2252 0.01% 70 2652 0.01% 71 2910 0.01% 72 3439 0.01% 73 3776 0.01% 74 4155 0.01% 75 4663 0.01% 76 5044 0.02% 77 5471 0.02% 78 6043 0.02% 79 6696 0.02% 80 7476 0.02% 81 8505 0.03% 82 9780 0.03% 83 10882 0.03% 84 11759 0.04% 85 13028 0.04% 86 13891 0.04% 87 15119 0.05% 88 16039 0.05% 89 17516 0.06% 90 18421 0.06% 91 20363 0.06% 92 22053 0.07% 93 23862 0.08% 94 25844 0.08% 95 27714 0.09% 96 28880 0.09% 97 30007 0.10% 98 32009 0.10% 99 33390 0.11% 100 35139 0.11% 101 36499 0.12% 102 38591 0.12% 103 41369 0.13% 104 43108 0.14% 105 45421 0.14% 106 47158 0.15% 107 48717 0.15% 108 50130 0.16% 109 51783 0.16% 110 53454 0.17% 111 56032 0.18% 112 57101 0.18% 113 59179 0.19% 114 62528 0.20% 115 64100 0.20% 116 66185 0.21% 117 68130 0.22% 118 69221 0.22% 119 70562 0.22% 120 71298 0.23% 121 73747 0.23% 122 75141 0.24% 123 76731 0.24% 124 80294 0.25% 125 81314 0.26% 126 83220 0.26% 127 86029 0.27% 128 86792 0.27% 129 87961 0.28% 130 89350 0.28% 131 90722 0.29% 132 91610 0.29% 133 94329 0.30% 134 95364 0.30% 135 97798 0.31% 136 99665 0.32% 137 101128 0.32% 138 102258 0.32% 139 105526 0.33% 140 105469 0.33% 141 108110 0.34% 142 110628 0.35% 143 111995 0.35% 144 114059 0.36% 145 115064 0.36% 146 118777 0.38% 147 257770 0.82% 148 110920 0.35% 149 110550 0.35% 150 27012813 85.59% 31561760 reads passed initial QC criterion=sequence-density sequence-density=2.19 sequence-density-rank=1 fanout-score=3.35 fanout-score-rank=20 prefix-density=2.82 prefix-fanout=2.6 sequence=GAACCGGAACCG criterion=fanout-score sequence-density=0.12 sequence-density-rank=28 fanout-score=7.93 fanout-score-rank=1 prefix-density=0.46 prefix-fanout=2.0 sequence=ATCTTGGCCTTGACATTGTCAATGGTGTCTGACGACTCAACCTCAAGGGTGATGGTCTTGCC criterion=sequence-density sequence-density=4.45 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=32 prefix-density=4.45 prefix-fanout=2.0 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.05 sequence-density-rank=30 fanout-score=260.87 fanout-score-rank=1 prefix-density=1.53 prefix-fanout=7.7 sequence=GCGGCGGCGGCTACGGTGGCAGCCGTGGAGGCTCCGGCGGCGGCAACTGGAGGGAGTGAATGGTGGGGCCCCTCGTGGCCAGTTATCCTTGTTACCTTTTATCTGTGATGTTATCGCTCCCGAGTATCCTAGATCTCGCTCCATCGCGTAGGGTTTGAGATGTTTAAGGGTTACCATTAGGTGTTTGTCCGTGATGCTACCTGTCGTGTGTTCCTGTTCTGTTCCGTTCGCTATCCCTATGAATGAATGAAAAAAGAATGAGC Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x GAACCGGAACCG -y CGGTTCCGGTTC -o ERR5262779 ERR5262779_1.fastq ERR5262779_2.fastq Input file: ERR5262779_1.fastq Paired file: ERR5262779_2.fastq trimmed: ERR5262779-trimmed-pair1.fastq, ERR5262779-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): GAACCGGAACCG -- paired 3' end adapter sequence (-y): CGGTTCCGGTTC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 11:17:47 2024 >> started Fri Dec 6 11:18:06 2024 >> done (18.668s) 15780880 read pairs processed; of these: 48 ( 0.00%) short read pairs filtered out after trimming by size control 159 ( 0.00%) empty read pairs filtered out after trimming by size control 15780673 (100.00%) read pairs available; of these: 104 ( 0.00%) trimmed read pairs available after processing 15780569 (100.00%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 23 1 0.00% 24 3 0.00% 25 0 0.00% 26 0 0.00% 27 0 0.00% 28 1 0.00% 29 0 0.00% 30 3 0.00% 31 3 0.00% 32 2 0.00% 33 0 0.00% 34 3 0.00% 35 6 0.00% 36 1 0.00% 37 4 0.00% 38 4 0.00% 39 4 0.00% 40 0 0.00% 41 0 0.00% 42 2 0.00% 43 1 0.00% 44 1 0.00% 45 4 0.00% 46 2 0.00% 47 1 0.00% 48 1 0.00% 49 207 0.00% 50 191 0.00% 51 230 0.00% 52 247 0.00% 53 238 0.00% 54 295 0.00% 55 307 0.00% 56 328 0.00% 57 296 0.00% 58 432 0.00% 59 445 0.00% 60 491 0.00% 61 507 0.00% 62 645 0.00% 63 618 0.00% 64 519 0.00% 65 568 0.00% 66 555 0.00% 67 652 0.00% 68 683 0.00% 69 799 0.01% 70 1261 0.01% 71 1224 0.01% 72 1509 0.01% 73 2131 0.01% 74 2726 0.02% 75 2940 0.02% 76 2028 0.01% 77 2124 0.01% 78 3728 0.02% 79 3861 0.02% 80 3138 0.02% 81 3288 0.02% 82 6226 0.04% 83 4485 0.03% 84 7242 0.05% 85 5927 0.04% 86 7240 0.05% 87 6205 0.04% 88 8761 0.06% 89 9073 0.06% 90 8383 0.05% 91 10310 0.07% 92 9708 0.06% 93 12876 0.08% 94 12650 0.08% 95 14289 0.09% 96 14139 0.09% 97 15295 0.10% 98 15761 0.10% 99 14212 0.09% 100 18952 0.12% 101 18800 0.12% 102 18806 0.12% 103 20520 0.13% 104 21904 0.14% 105 22777 0.14% 106 24822 0.16% 107 24116 0.15% 108 24351 0.15% 109 26672 0.17% 110 26956 0.17% 111 26925 0.17% 112 28992 0.18% 113 29814 0.19% 114 32565 0.21% 115 31553 0.20% 116 33436 0.21% 117 35108 0.22% 118 33857 0.21% 119 34112 0.22% 120 36060 0.23% 121 37672 0.24% 122 37794 0.24% 123 38796 0.25% 124 40607 0.26% 125 41059 0.26% 126 41695 0.26% 127 43393 0.27% 128 43766 0.28% 129 44320 0.28% 130 44853 0.28% 131 45388 0.29% 132 45941 0.29% 133 47511 0.30% 134 48094 0.30% 135 49489 0.31% 136 50127 0.32% 137 50389 0.32% 138 51417 0.33% 139 53164 0.34% 140 52905 0.34% 141 54049 0.34% 142 55024 0.35% 143 56238 0.36% 144 56841 0.36% 145 57839 0.37% 146 59118 0.37% 147 130380 0.83% 148 55409 0.35% 149 55205 0.35% 150 13499052 85.54% criterion=sequence-density sequence-density=0.16 sequence-density-rank=1 fanout-score=4.01 fanout-score-rank=34 prefix-density=0.22 prefix-fanout=3.0 sequence=GCCCAGGCGAGGCCGCCCACGAAGCAACGGTACTCAACTTCCGCCATTCCTCCCACTAAACCCTAACGAACCGGAACC criterion=fanout-score sequence-density=0.09 sequence-density-rank=17 fanout-score=230.48 fanout-score-rank=1 prefix-density=0.83 prefix-fanout=24.2 sequence=CTTCTTCTTGTC criterion=sequence-density sequence-density=0.63 sequence-density-rank=1 fanout-score=2.05 fanout-score-rank=36 prefix-density=0.63 prefix-fanout=2.0 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.10 sequence-density-rank=21 fanout-score=240.02 fanout-score-rank=1 prefix-density=1.08 prefix-fanout=22.7 sequence=CCGCCGCCGCCATC ERR5262779 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 11:19:16 Started mapping on | Dec 06 11:19:16 Finished on | Dec 06 11:21:57 Mapping speed, Million of reads per hour | 705.72 Number of input reads | 31561553 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 30289473 Uniquely mapped reads % | 95.97% Average mapped length | 291.91 Number of splices: Total | 30278184 Number of splices: Annotated (sjdb) | 28416173 Number of splices: GT/AG | 29855132 Number of splices: GC/AG | 357206 Number of splices: AT/AC | 20418 Number of splices: Non-canonical | 45428 Mismatch rate per base, % | 0.23% Deletion rate per base | 0.01% Deletion average length | 2.46 Insertion rate per base | 0.01% Insertion average length | 2.45 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 356442 % of reads mapped to multiple loci | 1.13% Number of reads mapped to too many loci | 1567 % of reads mapped to too many loci | 0.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.89% % of reads unmapped: other | 0.01% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 915638 915638 915638 N_multimapping 356442 356442 356442 N_noFeature 986769 29519559 1225949 N_ambiguous 618877 4010 89529 UnstrandedReadsAssigned:28683827 PositiveStrandReadsAssigned:765904 NegativeStrandReadsAssigned:28973995 Dataset is classified negative stranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR5262779 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR5262779-trimmed-pair1.fastq ERR5262779-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 31,561,553 reads, 29,374,227 reads pseudoaligned [quant] estimated average fragment length: 267.954 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,191 rounds 52973 ERR5262779.ke.tsv 35125 ERR5262779.se.tsv 88098 total ==> ERR5262779.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 669.625 0 0 PNS24247 1044 777.046 94.6651 5.78449 PNS24249 1928 1661.05 297.041 8.49096 PNS24246 1044 777.046 94.6651 5.78449 PNS24248 1044 777.046 94.6651 5.78449 PNS24244 1471 1204.05 188.964 7.45174 PNS24243 293 102.395 1 0.463707 KQK14069 1603 1336.05 44027.8 1564.69 KQK14071 474 236.84 595.007 119.286 ==> ERR5262779.se.tsv <== BRADI_1g14170v3 46916 BRADI_1g53295v3 215 BRADI_1g59795v3 656 BRADI_1g07683v3 0 BRADI_1g00485v3 42 BRADI_1g20270v3 1272 BRADI_1g74790v3 1701 BRADI_1g09890v3 0 BRADI_1g77505v3 378 BRADI_1g48960v3 0 ERR5262779 completed mapping pipeline successfully